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perceptions of pain. 20133697255
counselling on smoking. 20133719492
a microplate enzyme-linked immunosorbent assay for measuring antibody to anaplasma marginale in cattle serum.an enzyme-linked immunosorbent assay (elisa) method is described for measuring antibody against anaplasma marginale in cattle serum. this method was more sensitive and objective than a previously described elisa method for a. marginale and possible reasons for this are discussed. all 83 cattle experimentally infected with a. marginale (81) or a. centrale (2) developed demonstrable specific antibody but the serums of 98.8% of 839 cattle from cattle tick-free areas did not react by elisa; 378 seru ...19863729834
hypercalcemia associated with malignancy in a horse.hypercalcemia associated with malignancy was diagnosed in a 2-year-old thoroughbred filly admitted because of weight loss and reduced exercise tolerance of approximately 2 months' duration. laboratory findings included hypercalcemia, hypophosphatemia, anemia, marked neutrophilia with lymphopenia and eosinopenia, and normal immunoreactive parathyroid hormone concentration. at necropsy, a 53.6-kg tumor was located in the cranioventral aspect of the abdominal cavity. gross renal lesions were not no ...19863733508
[elisa on cells. review of current technics].immunoenzymological techniques are used to detect monoclonal antibodies directed against cell membrane antigens. the different materials used, the method of utilizing cells, the choice of the appropriate enzyme and the different technical variations are described. the advantages and disadvantages of each method are discussed.20133889783
igg bearing covalently bound c3b has enhanced bactericidal activity for escherichia coli 0111.the mechanism was sought by which bactericidal igg for e. coli 0111 (strain 12015) increases the bactericidal efficiency of c5b-9. igg did not affect the distribution of c3 deposition on the o-ag capsule and the outer membrane of 12015, suggesting that bactericidal igg was not directing complement activation to different sites on the bacterial surface. however, one-fifth of the c3 that was deposited in the presence of igg attached covalently to the antibody molecule. covalent complexes between p ...19853897448
[antonio carini, a student of ghislieri college]. 20143911354
platelets and coronary disease, round three. 20143918608
[abstracts of papers: 6th venezuelan congress of gastroenterology. maracaibo, july-august 1985]. 20143938747
statement on nutrition and your health: dietary guidelines for americans. 20143941224
soft drink consumption patterns of the u.s. population. 20143973329
[model concepts of disease formation and development from the viewpoint of psychotherapy]. 20143993099
parathyroid hormone and the altered vascular response to norepinephrine in uremia.patients with chronic renal failure manifest reduced pressor response to norepinephrine (ne); this abnormality is at least partly responsible for the autonomic nervous system dysfunction observed in these patients. since uremia is associated with increased levels of parathyroid hormone (pth) and since pth blunts the pressor effect of ne most likely via activation of prostaglandins, we have studied the relationship between blood levels of pth and the reduced pressor response to ne in 17 uremic pa ...20143993699
surface changes in intrauterine contraceptive devices after variable use.four used intrauterine contraceptive devices and two new control devices were studied by scanning electron microscopy. all intrauterine contraceptive devices were found to have surface deposits that differed not only from site to site on a given device but between devices. a finding of this study not previously described in the literature was the observation of a euhedral crystal rosette of an apatite mineral group [ca5(po4)3(f,cl,oh)] on the lippes loop that had been in situ for 15 years.20143993713
[bacteremia caused by intravascular percutaneous cannulas]. 20134021594
purification and characterization of two vascular endothelium effectors from fetal bovine retina, vitreous and serum.the purification and characterization of uridine and thymidine, two previously isolated vascular endothelium effectors, are described. these two effectors were purified from all dialyzable fractions of fetal bovine serum (fbs), vitreous, and retina. the present study demonstrates the stimulatory characteristic of uridine and thymidine on the proliferation of aortic endothelial cells. the stimulation appears to link to their utilization by the cells in a dialyzed fbs-dependent manner. in the pres ...19854029288
[immune-serological studies on hyperthyroidism treated with radioiodine]. 20144098959
ipsilateral afferents to the commissural zone of the fascia dentata, demonstrated in decommissurated rats by silver impregnation. 19714106860
effects of carbon dioxide on the alpha frequency and reaction time in humans. 20144169827
quantitative determination of secretory immunoglobulin a (siga) in middle ear effusions. 20144206200
histochemical observations on rumen mucosa structure and composition. 19694236739
nonspecific air pollution syndrome (naps). preliminary report. 20144249015
cytological analysis of the effect of methionine sulphoximine on protein metabolism of cells of the nervous system in vivo. 19684283665
cytological analysis of the effect of methionine sulphoximine on protein metabolism of cells of the nervous system in vivo. 19684283665
[enzyme histochemical studies of the nervous system. i. the content of some oxidoreductases in the cerebral ganglion of the earthworm (eisenia foetida) with special consideration of the neurosecretory system]. 20154298104
mechanism of adrenaline-induced lipolysis in adipose tissue. 19744363679
preparation of glutamate dehydrogenase from micrococcus aerogenes. 19704397848
clinical grand reounds: the many facets of hemophilia. 20144406153
clinical grand reounds: the many facets of hemophilia. 20144406153
[cervical spinal lesions]. 20154425335
[characteristics of bioenergetic processes in erythrocytes of patients with progressive muscular dystrophy]. 20154446546
studies on murine leprosy bacillus. x. studies on an atypical mycobacteria isolated from experimental murine leprosy mice. 19744449161
[comparative study of the therapeutic results and of clinical and psychosocial data from a medico-social guidance center for alcoholics]. 20154468707
purification of large crosslinked peptides from insoluble calf bone and skin collagens by hydroxyapatite chromatography. 19744473211
conversion of human hexosaminidase a to hexosaminidase "b" by crude vibrio cholerae neuraminidase preparations: merthiolate is the active factor. 19744473220
a nursing research workshop. 20154493005
a perspective on prophylaxis of dental caries by topical fluoride. 20144506529
facial neuralgia caused by unrecognized foreign bodies: report of case. 20154509705
legislating education in dentistry. 20154520738
[medical ethics with respect to the medical care structure]. 20154540933
suppressor cells in the rabbit appendix. 19744547314
transposed great arteries combined with totally anomalous pulmonary veins. a report of a successful correction. 20154551764
effects of oxidized spermine and acrolein on the transforming activity of t4 dna. 19724552901
effects of oxidized spermine and acrolein on the transforming activity of t4 dna. 19724552901
[studies on murine leprosy bacillus. x. rapid growing mycobacteria isolated from experimental murine leprosy mice (author's transl)]. 19744617060
isolation of a new rna polymerase-binding protein from sporulating bacillus subtilis.rna polymerase was precipitated from extracts of radioactively labeled vegetative and sporulating bacillus subtilis with antiserum prepared against vegetative core polymerase. the precipitates were solubilized and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. antiserum added to an extract of vegetative b. subtilis precipitated only the known subunits of core rna polymerase, but antiserum added to an extract of sporulating cells precipitated a new polypeptide of 70,000 da ...19734631355
[perirenal steatonecrosis or retroperitoneal necrosing panniculitis (apropos of a case)]. 20154652104
frequency of hepatitis b antigen in blood donors.routine testing of all blood donations for hepatitis b antigen by counter-immunoelectrophoresis was started in april 1971. the frequency of hbag carriers among apparently healthy volunteer blood donors in the toronto centre of the canadian red cross blood transfusion service was 15 in 10,000 with a male:female ratio of 6:1. excluding penitentiary inmates, the highest incidence was among young, male, non-anglo-saxon city dwellers.a system of notification of hbag-positive donors was developed, thr ...20154689555
change in transcription of repetitive dna sequences in n-methyl-n'-nitro-n-nitrosoguanidine-transformed hamster cells. 20154735236
change in transcription of repetitive dna sequences in n-methyl-n'-nitro-n-nitrosoguanidine-transformed hamster cells. 20154735236
[clinical use of anti-aggregative agents]. 20164771351
vertebrate collagen heterogeneity: a brief summary. 20164831113
long-term treatment of alcoholism with amitriptyline and emylcamate. a double-blind evaluation. 20164860508
renal allograft rejection simulated by arterial stenosis. 20164919685
blood flow through different regions of long bone measured by a bone-seeking radioisotopic method. 19714923134
health maintenance organization. 20164936249
unusual features in familial asphyxiating thoracic dysplasia (jeune's disease). 20165054317
[sarcomatoid stroma epitheliomas of the oropharynx and larynx]. 20165078883
hhai methylase and restriction endonuclease as probes for b to z dna conformational changes in d(gcgc) sequences.the capacity of the modification methylase (mhhai) and restriction endonuclease (hhai) form haemophilus haemolyticus to methylate and cleave, respectively, recognition sites which are in right-handed b or left-handed z structures was determined in vitro. plasmids containing tracts of (dc-dg) as well as numerous individual d(gcgc) sites distributed around the vector were studied. negative supercoiling was used to convert the (dc-dg) tracts (approximately 30 bp in length) from a right-handed to a ...19846093048
fimbriation of haemophilus species isolated from the respiratory tract of adults.twenty-two clinical isolates of haemophilus species were studied within two passages of their original isolation for the presence of fimbriae by negative-staining electron microscopy. six isolates were identified as fimbriated, including three strains of nontypable haemophilus influenzae, one strain of haemophilus parainfluenzae, and two strains of haemophilus haemolyticus. in fresh isolates of fimbriated strains of nontypable h. influenzae, approximately 40%-50% of cells had fimbriae; after fiv ...19846146651
the nucleotide sequence of is5 from escherichia coli.a 3-kb fragment of haemophilus haemolyticus dna which carries the hhaii restriction (r) and modification (m) genes has been cloned into the psti site of pbr322 (mann et al., 1978). when propagated in escherichia coli, it was observed that spontaneous insertions of is5 inactivated the restriction gene, producing r- mutants at a frequency of 10(-6). electron microscopy, restriction-site mapping and sequence analysis of two r- plasmids have demonstrated the presence of is5 at a single target site i ...19816269959
the nucleotide sequence of the hhaii restriction and modification genes from haemophilus haemolyticus.we have determined the nucleotide sequence of a cloned 1710-bp segment of haemophilus haemolyticus dna which contains the hhaii restriction (r) and modification (m) genes. the m gene is 513 bp in length and the rgene is 681 bp in length. both are in the same reading frame, being separated by a 21-bp region. a ribosome-binding site is identified in front of each gene, but no haemophilus promoter is apparent on the cloned fragment. transcription originates from a plasmid promotor and proceeds in t ...19836315538
relative proportions of haemophilus species in the throat of healthy children and adults.to determine normal proportions of pharyngeal haemophilus species, qualitative and quantitative mapping of the species in the pharynx of ten healthy children and ten healthy adults was carried out using a selective and a non-selective medium. haemophilus organisms were present in all samples, comprising approximately 10% of the total cultivable flora (range 0.6-36.9%). haemophilus parainfluenzae was a member of the normal flora throughout life, constituting 74% of pharyngeal haemophilus organism ...19846332018
binding of human hemoglobin by haemophilus influenzae.binding of biotinylated human hemoglobin to haemophilus influenzae was detected when organisms were grown in heme-deplete, but not heme-replete, conditions. hemoglobin binding was completely inhibited by a 100-fold excess of unlabelled human hemoglobin or human hemoglobin complexed with human haptoglobin. binding was only partially inhibited by rat hemoglobin, bovine hemoglobin, human globin, and bovine globin, and not at all by heme, human serum albumin, bovine serum albumin, human transferrin, ...19948020748
genetic characterization of a cryptic genospecies of haemophilus causing urogenital and neonatal infections.in recent years, reports originating from several areas of the world have identified biotype iv strains of haemophilus influenzae as a cause of serious urogenital, neonatal, and mother-infant infections. preliminary analysis of a sample of biotype iv isolates found evidence for a cryptic genospecies of haemophilus (r. quentin, a. goudeau, r. j. wallace, jr., a. l. smith, r. k. selander, and j. m. musser. j. gen. microbiol. 136:1203-1209, 1990). eighteen biotype iv strains assigned to the cryptic ...19938099082
functional roles of the conserved aromatic amino acid residues at position 108 (motif iv) and position 196 (motif viii) in base flipping and catalysis by the n6-adenine dna methyltransferase from thermus aquaticus.the dna methyltransferase (mtase) from thermus aquaticus (m.taqi) catalyzes the transfer of the activated methyl group of s-adenosyl-l-methionine to the n6 position of adenine within the double-stranded dna sequence 5'-tcga-3'. to achieve catalysis m.taqi flips the target adenine out of the dna helix. on the basis of the three-dimensional structure of m.taqi in complex with the cofactor and its structural homology to the c5-cytosine dna mtase from haemophilus haemolyticus, tyr 108 and phe 196 we ...19999931007
zebularine: a novel dna methylation inhibitor that forms a covalent complex with dna methyltransferases.mechanism-based inhibitors of enzymes, which mimic reactive intermediates in the reaction pathway, have been deployed extensively in the analysis of metabolic pathways and as candidate drugs. the inhibition of cytosine-[c5]-specific dna methyltransferases (c5 mtases) by oligodeoxynucleotides containing 5-azadeoxycytidine (azadc) and 5-fluorodeoxycytidine (fdc) provides a well-documented example of mechanism-based inhibition of enzymes central to nucleic acid metabolism. here, we describe the int ...200212206775
structural basis of icf-causing mutations in the methyltransferase domain of dnmt3b.mutations in the gene encoding for a de novo methyltransferase, dnmt3b, lead to an autosomal recessive immunodeficiency, centromeric instability and facial anomalies (icf) syndrome. to analyse the protein structure and consequences of icf-causing mutations, we modelled the structure of the dnmt3b methyltransferase domain based on haemophilus haemolyticus protein in complex with the cofactor adomet and the target dna sequence. the structural model has a two-subdomain fold where the dna-binding re ...200212601140
a classification of the hemolytic bacteria of the genus haemophilus: haemophilus haemolyticus bergey et al. and haemophilus parahaemolyticus nov spec. 195313069455
presence of copper- and zinc-containing superoxide dismutase in commensal haemophilus haemolyticus isolates can be used as a marker to discriminate them from nontypeable h. influenzae isolates.respiratory isolates of haemophilus haemolyticus are regularly misclassified as nontypeable (nt) haemophilus influenzae due to an aberrant hemolytic reaction on blood agar, with implications for treatment. the presence of sodc or its cognate protein, copper-zinc superoxide dismutase, can distinguish respiratory isolates of h. haemolyticus from nt h. influenzae with 100% accuracy.200616988021
haemophilus haemolyticus: a human respiratory tract commensal to be distinguished from haemophilus influenzae.haemophilus influenzae is a common pathogen in adults with chronic obstructive pulmonary disease (copd). in a prospective study, selected isolates of apparent h. influenzae had an altered phenotype. we tested the hypothesis that these variant strains were genetically different from typical h. influenzae.200717152011
horizontal gene transfer of ftsi, encoding penicillin-binding protein 3, in haemophilus influenzae.horizontal gene transfer has been identified in only a small number of genes in haemophilus influenzae, an organism which is naturally competent for transformation. this report provides evidence for the genetic transfer of the ftsi gene, which encodes penicillin-binding protein 3, in h. influenzae. mosaic structures of the ftsi gene were found in several clinical isolates of h. influenzae. to identify the origin of the mosaic sequence, complete sequences of the corresponding gene from seven type ...200717325223
airway bacterial concentrations and exacerbations of chronic obstructive pulmonary disease.increased bacterial concentration (load) in the lower airways and new bacterial strain acquisition have been posited as mechanisms for chronic obstructive pulmonary disease (copd) exacerbations. bacterial concentrations are higher during exacerbation than during stable disease; however, these studies are cross sectional and devoid of strain typing.200717478618
pharyngeal colonization dynamics of haemophilus influenzae and haemophilus haemolyticus in healthy adult carriers.haemophilus influenzae is an important cause of respiratory infections, including acute otitis media, sinusitis, and chronic bronchitis, which are preceded by asymptomatic h. influenzae colonization of the human pharynx. the aim of this study was to describe the dynamics of pharyngeal colonization by h. influenzae and an intimately related species, haemophilus haemolyticus, in healthy adults. throat specimens from four healthy adult carriers were screened for haemophilus species; 860 isolates we ...200717687018
relationships of nontypeable haemophilus influenzae strains to hemolytic and nonhemolytic haemophilus haemolyticus strains.haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while h. haemolyticus, the closest phylogenetic relative of h. influenzae, is arguably a strict pharyngeal commensal. a hemolytic phenotype has historically differentiated h. haemolyticus from h. influenzae, but the recent recognition of significant nonhemolytic h. haemolyticus colonization has decreased this trait's resolvability. given this and the potential of recombination between the species, we examined t ...200818039799
delineation of the species haemophilus influenzae by phenotype, multilocus sequence phylogeny, and detection of marker genes.to obtain more information on the much-debated definition of prokaryotic species, we investigated the borders of haemophilus influenzae by comparative analysis of h. influenzae reference strains with closely related bacteria including strains assigned to haemophilus haemolyticus, cryptic genospecies biotype iv, and the never formally validated species "haemophilus intermedius". multilocus sequence phylogeny based on six housekeeping genes separated a cluster encompassing the type and the referen ...200919060144
detection of cryptic genospecies misidentified as haemophilus influenzae in routine clinical samples by assessment of marker genes fuck, hap, and sodc.clinical isolates of haemophilus influenzae were assessed for the presence of fuck, hap, and sodc by hybridization with gene-specific probes, and isolates diverging from the expected h. influenzae genotype were characterized by phenotype and 16s rrna gene sequencing. two of 480 isolates were finally classified as variant strains ("nonhemolytic haemophilus haemolyticus").200919535530
a comparative study of the biological characters and pathogenesis of bacillus x (sternberg), bacillus icteroides (sanarelli), and the hog-cholera bacillus (salmon and smith).1. bacillus x (sternberg) belongs to the colon group. 2. bacillus icteroides (sanarelli) is a member of the hog-cholera group. 3. the various channels of infection, the duration of the disease and the gross and microscopical lesions in mice, guinea-pigs and rabbits are the same for bacillus icteroides and the hog-cholera bacillus. 4. the clinical symptoms and the lesions observed in dogs inoculated intravenously with bacillus icteroides, are reproduced in these animals by infection with the hog- ...190019866945
studies in the common cold : i. observations of the normal bacterial flora of nose and throat with variations occurring during colds.1. the normal bacterial flora of the nose and throat of thirteen individuals has been studied over periods ranging from 5 to 9 months. 2. observations have been made of qualitative and quantitative changes in the flora occurring in the course of colds and of throat infections appearing in the group. 3. the normal basic nasal flora includes staphylococcus albus, diphtheroids, and for certain individuals staphylococcus aureus and citreus. occasional transient bacteria are gram-negative cocci and n ...192619869133
a growth inhibitory substance for the influenza group of organisms in the blood of various animal species : the use of the blood of various animals as a selective medium for the detection of hemolytic streptococci in throat cultures.1. 5 per cent sheep blood agar is a selective medium for beta hemolytic streptococci in throat cultures since sheep blood inhibits the growth of bacillus x (h. hemolyticus) and b. parainfluenzae hemolyticus. the growth of h. influenzae is also inhibited by sheep blood. 2. the inhibitory action of sheep blood resides in the erythrocytes and is thermolabile. disruption of the cell by laking has no effect upon the inhibitor. 3. the bloods of animals closely related to the sheep, such as the goat an ...193819870730
prevalence of the sodc gene in nontypeable haemophilus influenzae and haemophilus haemolyticus by microarray-based hybridization.the sodc gene has been reported to be a useful marker for differentiating nontypeable (nt) haemophilus influenzae from haemophilus haemolyticus in respiratory-tract samples, but discrepancies exist as to the prevalence of sodc in nt h. influenzae. therefore, we used a microarray-based, "library-on-a-slide" method to differentiate the species and found that 21 of 169 (12.4%) nt h. influenzae strains and all 110 (100%) h. haemolyticus strains possessed the sodc gene. multilocus sequence analysis c ...201020042621
antimicrobial characterisation of cem-101 activity against respiratory tract pathogens, including multidrug-resistant pneumococcal serogroup 19a isolates.cem-101 is a novel fluorinated macrolide-ketolide with potent activity against bacterial pathogens that are susceptible or resistant to other macrolide-lincosamide-streptogramin b (mls(b))-ketolide agents. cem-101 is being developed for oral and parenteral use in moderate to moderately severe community-acquired bacterial pneumonia. the objective of this study was to assess the activity of cem-101 and comparators against contemporary respiratory tract infection (rti) isolates. a worldwide sample ...201020211548
haemophilus influenzae and haemophilus haemolyticus in tonsillar cultures of adults with acute pharyngotonsillitis.the aim of this study was to evaluate the clinical implication of haemophilus haemolyticus, one of the closest relative of haemophilus influenzae, on acute pharyngotonsillitis.201020392581
prevalence of haemophilus influenzae type b genetic islands among clinical and commensal h. influenzae and h. haemolyticus isolates.five genetic islands (higi) found in haemophilus influenzae type b strain eagan were used as hybridization probes on type b, haemophilus haemolyticus, and nontypeable h. influenzae (nthi) isolates. higi2 and higi7 were significantly more prevalent in nthi isolates from children with otitis media than in those from the throats of healthy children.201020463164
nasopharyngeal carriage of haemophilus haemolyticus in otitis-prone and healthy children.haemophilus haemolyticus is often incorrectly categorized as nontypeable haemophilus influenzae (nthi) upon culture. pcr analyses of 266 nthi-like nasopharyngeal isolates from children with and without recurrent acute otitis media (raom) revealed that 11.7% were h. haemolyticus and 9.4% gave equivocal results. children with raom were more likely to carry h. haemolyticus.201020463165
haemophilus influenzae outer membrane protein p6 molecular characterization may not differentiate all strains of h. influenzae from h. haemolyticus.distinguishing nontypeable haemophilus influenzae and haemophilus haemolyticus isolates by outer membrane protein (omp) p6 gene sequencing is complicated by sequence variants in isolates. further testing using rapid nh and multilocus sequence analysis may not help identify some isolates. translated omp p6 gene sequences are not conserved among all isolates presumed to be h. influenzae.201020686092
prevalence of genetic differences in phosphorylcholine expression between nontypeable haemophilus influenzae and haemophilus haemolyticus.although non-typeable (nt) haemophilus influenzae and haemophilus haemolyticus are closely related human commensals, h. haemolyticus is non-pathogenic while nt h. influenzae is an important cause of respiratory tract infections. phase-variable phosphorylcholine (chop) modification of lipooligosaccharide (los) is a nt h. influenzae virulence factor that, paradoxically, may also promote complement activation by binding c-reactive protein (crp). crp is known to bind more to chop positioned distally ...201021073698
medical microbiologythe genus haemophilus includes a number of species that cause a wide variety of infections but share a common morphology and a requirement for blood-derived factors during growth that has given the genus its name. haemophilus influenzae, the major pathogen, can be separated into encapsulated or typable strains, of which there are seven types (a through f including e') based on the antigenic structure of the capsular polysaccharide, and unencapsulated or nontypable strains. type b h influenzae is ...199621413340
genome sequences for five strains of the emerging pathogen haemophilus haemolyticus.we report the first whole-genome sequences for five strains, two carried and three pathogenic, of the emerging pathogen haemophilus haemolyticus. preliminary analyses indicate that these genome sequences encode markers that distinguish h. haemolyticus from its closest haemophilus relatives and provide clues to the identity of its virulence factors.201121952546
evaluation of new biomarker genes for differentiating haemophilus influenzae from haemophilus haemolyticus.pcr detecting the protein d (hpd) and fuculose kinase (fuck) genes showed high sensitivity and specificity for identifying haemophilus influenzae and differentiating it from h. haemolyticus. phylogenetic analysis using the 16s rrna gene demonstrated two distinct groups for h. influenzae and h. haemolyticus.201222301020
an invasive haemophilus haemolyticus isolate. 201222301029
molecular surveillance of true nontypeable haemophilus influenzae: an evaluation of pcr screening assays.unambiguous identification of nontypeable haemophilus influenzae (nthi) is not possible by conventional microbiology. molecular characterisation of phenotypically defined nthi isolates suggests that up to 40% are haemophilus haemolyticus (hh); however, the genetic similarity of nthi and hh limits the power of simple molecular techniques such as pcr for species discrimination.201222470516
culture and pcr detection of haemophilus influenzae and haemophilus haemolyticus in australian indigenous children with bronchiectasis.a pcr for protein d (hpd#3) was used to differentiate nontypeable haemophilus influenzae (nthi) from haemophilus haemolyticus. while 90% of nasopharyngeal specimens and 100% of lower-airway specimens from 84 indigenous australian children with bronchiectasis had phenotypic nthi isolates confirmed as h. influenzae, only 39% of oropharyngeal specimens with phenotypic nthi had h. influenzae. the nasopharynx is therefore the preferred site for nthi colonization studies, and nthi is confirmed as an i ...201222553240
haemophilus haemolyticus isolates causing clinical disease.we report seven cases of haemophilus haemolyticus invasive disease detected in the united states, which were previously misidentified as nontypeable haemophilus influenzae. all cases had different symptoms and presentations. our study suggests that a testing scheme that includes reliable pcr assays and standard microbiological methods should be used in order to improve h. haemolyticus identification.201222573587
prevalence and mechanisms of β-lactam resistance in haemophilus haemolyticus.to compare the phenotypic and genotypic β-lactam resistance profiles of non-typeable haemophilus influenzae (nthi) and the closely phylogenetically related haemophilus haemolyticus.201323315478
maldi-tof ms distinctly differentiates nontypable haemophilus influenzae from haemophilus haemolyticus.nontypable haemophilus influenzae (nthi) and haemophilus haemolyticus exhibit different pathogenicities, but to date, there remains no definitive and reliable strategy for differentiating these strains. in this study, we evaluated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) as a potential method for differentiating nthi and h. haemolyticus. the phylogenetic analysis of concatenated 16s rrna and recombinase a (reca) gene sequences, outer membrane pr ...201323457514
microbiological diagnosis and antibiotic therapy in patients with community-acquired pneumonia and acute copd exacerbation in daily clinical practice: comparison to current guidelines.the aim of this secondary analysis was to evaluate current microbiological approaches, microbiology, and antibiotic therapy in patients with community-acquired pneumonia (cap) and acute exacerbation of chronic obstructive pulmonary disease (aecopd) in clinical practice and to compare them with current international guidelines.201323564195
pcr screening for the n526k substitution in isolates of haemophilus influenzae and haemophilus haemolyticus.firstly, to evaluate the current pbp3-s primers of hasegawa et al. (microb drug resist 2003; 9: 39-46) and develop new primers for the amplification of n526 in isolates of haemophilus haemolyticus. secondly, to develop a new pcr assay for the detection (by amplification) of the n526k substitution, encoded by either the aaa or aag single nucleotide polymorphism (snp) at position 1576-1578 of the ftsi gene, in isolates of both haemophilus influenzae and h. haemolyticus.201323645583
identification of haemophilus influenzae and haemophilus haemolyticus by matrix-assisted laser desorption ionization-time of flight mass spectrometry.generally accepted laboratory methods that have been used for decades do not reliably distinguish between h. influenzae and h. haemolyticus isolates. h. haemolyticus strains are often incorrectly identified as nontypeable haemophilus influenzae (nthi). to distinguish h. influenzae from h. haemolyticus we have created a new database on the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) bio-typer 2 and compared the results with routine determination of ...201424019162
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
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