Publications

first european case of gastroenteritis and bacteremia due to vibrio hollisae.	vibrio hollisae is a pathogenic vibrio species known to cause gastroenteritis in humans after the consumption of shellfish. all cases of infection reported previously were restricted to the atlantic and pacific coasts of the united states. a case of gastroenteritis and bacteremia in a previously healthy 76-year-old man who ate cockles from the quiberon bay in brittany, france, is described. this is the first report of vibrio hollisae infection in europe.	1996	8997558
identification of major antigenic proteins of pasteurella piscicida.	two different antigenic protein-coding clones (ppa1 and ppa2) were isolated using anti-pasteurella piscicida rabbit serum from a genomic dna library of p. piscicida strain kp9038. the ppa1 and ppa2 expressed 7 kda and 45 kda proteins in escherichia coli, respectively, and the molecular sizes of these expressed proteins are the same as these of the major antigenic proteins of p. piscicida. ppa1 encodes a protein of 83 amino acids residues, which is similar to the bacterial lipoprotein. comparison ...	1997	9441863
response of pathogenic vibrio species to high hydrostatic pressure.	vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance.	1999	10347079
analysis of gyrb and toxr gene sequences of vibrio hollisae and development of gyrb- and toxr-targeted pcr methods for isolation of v. hollisae from the environment and its identification.	isolation of vibrio hollisae strains, particularly from the environment, is rare. this may be due, in part, to the difficulty encountered when using conventional biochemical tests to identify the microorganism. in this study, we evaluated whether two particular genes may be useful for the identification of v. hollisae. the two genes are presumed to be conserved among the bacterial species (gyrb) or among the species of the genus vibrio (toxr). a portion of the gyrb sequence of v. hollisae was cl ...	2000	10919814
vibrio calviensis sp. nov., a halophilic, facultatively oligotrophic 0.2 microm-fiiterabie marine bacterium.	a gram-negative, facultatively anaerobic, straight to slightly curved rod-shaped bacterium (re35f/12t) sensitive to vibriostatic agent o/129 was previously isolated from sea water (western mediterranean sea, bay of calvi, corsica, france) by 0.2 microm-membrane filtration. strain re35/f12t (= cip 107077t = dsm 14347t) was facultatively oligotrophic, halophilic, required na+ for growth and produced acid but no gas from d-glucose under anaerobic conditions. comparative 165 rrna gene-sequence analy ...	2002	11931167
enterovibrio norvegicus gen. nov., sp. nov., isolated from the gut of turbot (scophthalmus maximus) larvae: a new member of the family vibrionaceae.	twenty-two isolates originating from the gut of healthy cultured turbot larvae in norway were investigated using a polyphasic approach. amplified fragment length polymorphism fingerprinting analysis showed that the isolates have typical patterns and form two main groups. phylogenetic analysis revealed that the isolates belong to the gamma-proteobacteria, with vibrio hollisae as their closest neighbour. dna-dna hybridization, chemotaxonomic and phenotypic analyses further proved that these isolat ...	2002	12508862
reclassification of vibrio hollisae as grimontia hollisae gen. nov., comb. nov.	the taxonomic positions of three representative strains of vibrio hollisae (lmg 17719(t), lmg 21416 and lmg 21538) were investigated by means of 16s rdna sequences and phenotypic data. v. hollisae strains (genbank/embl accession nos aj514909-aj514911) shared 99.5 % 16s rdna sequence similarity, but had only 94.6 % similarity to their closest phylogenetic neighbour, enterovibrio norvegicus. 16s rdna sequence similarity of v. hollisae and vibrio cholerae was only 91 %. these results suggest that v ...	2003	13130058
use of reca as an alternative phylogenetic marker in the family vibrionaceae.	this study analysed the usefulness of reca gene sequences as an alternative phylogenetic and/or identification marker for vibrios. the reca sequences suggest that the genus vibrio is polyphyletic. the high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. photobacterium species clustered together and apparently nested within vibrios, while grimontia hollisae was apart from other vibrios. within the vibrios, vibrio cholerae and vibrio mimi ...	2004	15143042
biodiversity of vibrios.	vibrios are ubiquitous and abundant in the aquatic environment. a high abundance of vibrios is also detected in tissues and/or organs of various marine algae and animals, e.g., abalones, bivalves, corals, fish, shrimp, sponges, squid, and zooplankton. vibrios harbour a wealth of diverse genomes as revealed by different genomic techniques including amplified fragment length polymorphism, multilocus sequence typing, repetetive extragenic palindrome pcr, ribotyping, and whole-genome sequencing. the ...	2004	15353563
rapid phenotypic characterization of vibrio isolates by pyrolysis metastable atom bombardment mass spectrometry.	pyrolysis mass spectrometry was investigated for rapid characterization of food-borne bacterial pathogens. nine isolates of vibrio parahaemolyticus and one isolate each of vibrio fluvialis, vibrio hollisae, and vibrio vulnificus were analyzed. pyrolysis mass spectra, generated via an alternative ionization method, metastable atom bombardment, were subject to principal component-discriminant analysis. the spectral patterns were used to distinguish vibrio isolates differing in species, serotype an ...	2005	16096691
identification and transcriptional organization of aerobactin transport and biosynthesis cluster genes of vibrio hollisae.	we had previously reported that vibrio hollisae produces aerobactin in response to iron starvation. in the present study, we identified in v. hollisae atcc33564 the aerobactin system cluster which consists of eight genes, hatcdb, iucabcd and iuta. the hatcdb genes encode proteins homologous to components of bacterial atp binding cassette transport systems for ferric aerobactin. the iucabcd and iuta orthologs code for aerobactin biosynthesis enzymes and the ferric aerobactin receptor, respectivel ...	2006	16809025
rugosity in grimontia hollisae.	grimontia hollisae, formerly vibrio hollisae, produces both smooth and rugose colonial variants. the rugose colony phenotype is characterized by wrinkled colonies producing copious amounts of exopolysaccharide. cells from a rugose colony grown at 30 degrees c form rugose colonies, while the same cells grown at 37 degrees c form smooth colonies, which are characterized by a nonwrinkled, uncrannied appearance. stress response studies revealed that after exposure to bleach for 30 min, rugose surviv ...	2007	17189437
severe gastroenteritis and hypovolemic shock caused by grimontia (vibrio) hollisae infection.	vibrio hollisae is a halophilic species that was recently reclassified as grimontia hollisae. this organism is known to cause moderate to severe cases of gastroenteritis. we report a case of an individual who suffered a more severe form of this disease, presenting with profound hypotension and acute renal failure, secondary to hypovolemic shock.	2007	17704283
genes encoding the vibrio harveyi haemolysin (vhh)/thermolabile haemolysin (tlh) are widespread in vibrios.	v. harveyi vhh haemolysin, which shows high homology to the tlh haemolysin (the identities of their deduced amino acid sequences are up to 85.6%), is a putative virulence factor to marine cultured fish. a vhh probe, which is specific to v. harveyi vhha haemolysin gene, was used to screen ecor i digests of total dna from 57 vibrio strains, including 26 vibrio type strains, 20 v. harveyi isolates and 11 v. parahaemolyticus isolates. as a result, 1 strong hybridisation band was detected in 13 type ...	2007	18062266
grimontia hollisae, a potential agent of gastroenteritis and bacteraemia in the mediterranean area.	vibrio hollisae was first described in 1982 as an agent of diarrhoea and was reclassified in 2003 into a novel genus as grimontia hollisae. we report the first case of g. hollisae bacteraemia in the mediterranean area, in an 81-year-old man with a severe gastroenteritis and hepatitis following the consumption of raw oysters. the incidence of this micro-organism as an agent of gastroenteritis may be underestimated because it may not be detected using routine culture conditions.	2009	19089475
vibrios as causal agents of zoonoses.	vibrios are gram-negative rod-shaped bacteria that are widespread in the coastal and estuarine environments. some species, e.g. vibrio anguillarum and v. tapetis, comprise serious pathogens of aquatic vertebrates or invertebrates. other groups, including grimontia (=vibrio) hollisae, photobacterium (=vibrio) damselae subsp. damselae, v. alginolyticus, v. harveyi (=v. carchariae), v. cholerae, v. fluvialis, v. furnissii, v. metschnikovii, v. mimicus, v. parahaemolyticus and v. vulnificus, may cau ...	2010	19342185
rapid and specific detection of the thermostable direct hemolysin gene in vibrio parahaemolyticus by loop-mediated isothermal amplification.	several investigators have reported that thermostable direct hemolysin (tdh) and tdh-related hemolysin are important virulence factors of vibrio parahaemolyticus, but it has been difficult to detect these factors rapidly in seafood and other environmental samples. a novel nucleic acid amplification method, termed the loop-mediated isothermal amplification (lamp), which amplifies dna with high specificity and rapidity under isothermal conditions, was applied. in this study, we designed tdh gene-s ...	2009	19435222
rna colony blot hybridization method for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria.	a species-specific rna colony blot hybridization protocol was developed for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria in environmental water samples. bacterial colonies on selective or nonselective plates were lysed by sodium dodecyl sulfate, and the lysates were immobilized on nylon membranes. a fluorescently labeled oligonucleotide probe targeting a phylogenetic signature sequence of 16s rrna of v. cholerae and v. mimicus was hybridized to rrna molecules immobilized ...	2009	19561182
development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes of vibrio parahaemolyticus and related vibrio species.	thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) are the major virulence determinants of vibrio parahaemolyticus. trh is further differentiated into trh1 and trh2 on the basis of genetic and phenotypic differences. we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for sensitive and rapid detection of the tdh, trh1, and trh2 genes of v. parahaemolyticus. the lamp assay was designed for both combined and individual detection of the tdh, ...	2010	19966027
rapid identification and characterization of vibrio species using whole-cell maldi-tof mass spectrometry.	vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. this work aimed at evaluating the use of maldi-tof mass spectrometry for the rapid identification of vibrio (v.) spp. as an advantageous application to rapidly discriminate the most important vibrio spp. and distinguish vibrio spp. from closely related bacterial species like photobacterium damselae and grim ...	2010	20059616
purification, crystallization and preliminary x-ray analysis of a thermostable direct haemolysin from grimontia hollisae.	vibrio hollisae, a halophilic species recently reclassified as grimontia hollisae, is a causative agent of gastroenteritis and septicaemia. one important pathogenic vibrio factor, thermostable direct haemolysin (tdh), has been purified and crystallized in two crystal forms using the vapour-diffusion method. the crystals belonged to an orthorhombic space group, with unit-cell parameters a = 104.8, b = 112.4, c = 61.3 å and a = 122.9, b = 123.3, c = 89.8 å. the crystals contained either four or ei ...	2011	21301091
site-directed mutations of thermostable direct hemolysin from grimontia hollisae alter its arrhenius effect and biophysical properties.	recombinant thermostable direct hemolysin from grimontia hollisae (gh-rtdh) exhibits paradoxical arrhenius effect, where the hemolytic activity is inactivated by heating at 60 °c but is reactivated by additional heating above 80 °c. this study investigated individual or collective mutational effect of tyr53, thr59, and ser63 positions of gh-rtdh on hemolytic activity, arrhenius effect, and biophysical properties. in contrast to the gh-rtdh wild-type (gh-rtdh(wt)) protein, a 2-fold decrease of he ...	2011	21494434
cloning of a novel collagenase gene from the gram-negative bacterium grimontia (vibrio) hollisae 1706b and its efficient expression in brevibacillus choshinensis.	the collagenase gene was cloned from grimontia (vibrio) hollisae 1706b, and its complete nucleotide sequence was determined. nucleotide sequencing showed that the open reading frame was 2,301 bp in length and encoded an 84-kda protein of 767 amino acid residues. the deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, the hexxh motif. g. hollisae collagenase showed 60 and 59% amino acid sequence identities to vibrio parahaemolyticus and v ...	2011	21515782
Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach.	Vibrios are a group of major foodborne pathogens widely distributed in marine environment. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the pathogenic species of Vibrio that pose the greatest threat to human health. However, other vibrios, e.g. Vibrio alginolyticus, Vibrio mimicus and Grimontia hollisae, apparently less relevant in the group of foodborne pathogens, have been sporadically found in outbreaks. For seafood safety and economic purposes, a rapid and powerful met ...	2011	22177227
grimontia marina sp. nov., a marine bacterium isolated from the yellow sea.	a novel species belonging to the genus grimontia is described in this study. a gram-negative, chemoheterotrophic, obligately aerobic, catalase- and oxidase-positive, motile by a single polar flagellum, and rod-shaped bacterium, designated imcc5001(t), was isolated from surface seawater of the yellow sea. strain imcc5001(t) grew optimally at 30°c in the presence of 3.5% nacl. phylogenetic analysis based on 16s rrna gene sequences showed that the isolate was related most closely to grimontia holli ...	2012	22367954
novel metagenome-derived, cold-adapted alkaline phospholipase with superior lipase activity as an intermediate between phospholipase and lipase.	a novel lipolytic enzyme was isolated from a metagenomic library obtained from tidal flat sediments on the korean west coast. its putative functional domain, designated mplag, showed the highest similarity to phospholipase a from grimontia hollisae cip 101886, though it was screened from an emulsified tricaprylin plate. phylogenetic analysis showed that mplag is far from family i.6 lipases, including staphylococcus hyicus lipase, a unique lipase which can hydrolyze phospholipids, and is more evo ...	2012	22544255
the thermostable direct hemolysin from grimontia hollisae causes acute hepatotoxicity in vitro and in vivo.	g. hollisae thermostable direct hemolysin (gh-tdh) is produced by most strains of g. hollisae. this toxin has been reported to be absorbed in the intestines in humans. secondary liver injury might be caused by venous return of the toxin through the portal system. we aimed to firstly analyze the in vitro and in vivo hepatotoxicity of gh-tdh.	2013	23437095
chlorophyll a might structure a community of potentially pathogenic culturable vibrionaceae. insights from a one-year study of water and mussels surveyed on the french atlantic coast.	the present study focused on the isolation of culturable bacteria from mussels and sea water to identify vibrionaceae potentially pathogenic for humans. three sites located on the french atlantic coast were monitored monthly (twice each month during summer) for 1 year. environmental parameters were surveyed (water temperature, salinity, turbidity, chlorophyll a) and bacteria were detected by culture and identified by api 20e(®) systems (biomérieux) and pcr. a total of seven species were detected ...	2010	23766015
grimontia indica ak16(t), sp. nov., isolated from a seawater sample reports the presence of pathogenic genes similar to vibrio genus.	grimontia indica strain ak16(t) sp. nov. is the type strain of g. indica sp. nov. a new species within the genus grimontia. this strain, whose genome is described here, was isolated from seawater sample collected from southeast coast of palk bay, india. g. indica ak16(t) is a gram-negative, facultative aerobic rod shaped bacterium. there are only two other strains in the genus grimontia one of which, grimontia hollisae cip 101886(t), is a reported human pathogen isolated from human stool sample ...	2014	24465608
potential antitumor therapeutic application of grimontia hollisae thermostable direct hemolysin mutants.	we report on the preparation of a new type of immunotoxin by conjugation of an epidermal growth factor receptor (egfr)-binding peptide and an r46e mutation of thermostable direct hemolysin from grimontia hollisae, (gh-tdh(r) (46e) /eb). the hybrid immunotoxin was purified to homogeneity and showed a single band with slight slower mobility than that of gh-tdh(r) (46e) . cytotoxicity assay of gh-tdh(r) (46e) /eb on egfr highly, moderately, low, and non-expressed cells, a431, mda-mb-231, hela, and ...	2015	25640743
hydrogen peroxide causes vibrio vulnificus bacteriolysis accelerated by sulfonyl fluoride compounds.	induction of bacteriolysis of vibrio vulnificus cells by 10 mm hydrogen peroxide (h(2)o(2)) was analyzed. all vibrio species examined, except for vibrio hollisae, were lysed by 10 mm h(2)o(2). bacteriophage induction was not the cause of h(2)o(2)-induced bacteriolysis. autolysis is also known to cause bacteriolysis. vvps protein is a serine protease of v. vulnificus essential for autolysis. vvps mutant underwent h(2)o(2)-induced bacteriolysis in the same manner as the wild type. protease inhibit ...	2015	26316164
grimontia celer sp. nov., from sea water.	strain 96-237t, a gram-reaction-negative, curved- to spiral-shaped motile bacterium, isolated from coastal marine water, was found to be related to species of the genus grimontia by 16s rrna gene sequence comparison, sharing 98.3 % similarity to grimontia marina cect 8713tand 98.8 % to 'grimontiaindica' ak16. phenotypic analysis revealed that strain 96-237t is slightly halophilic, mesophilic and facultatively anaerobic, fermenting d-glucose, d-ribose, d-mannose, d-mannitol, maltose and sucrose. ...	2016	27125858