Publications

reactivity of the imino acids formed in the amino acid oxidase reaction.	the reactivity of the imino acids formed in the d- or l-amino acid oxidase reaction was studied. it was found that: (1) when imino acids reacted with the alpha-amino group of glycine or other amino acids, transimination yielded derivatives less stable to hydrolysis than the parent imino acids. in contrast, when imino acids reacted with the epsilon-amino group of lysine or other primary amines, transimination yielded derivatives more stable to hydrolysis than the parent imino acids. (2) imino aci ...	1979	33698
laboratory studies with bl-s 578 (cefadroxil) a new broad-spectrum orally active cephalosporin.	bl-s 578 (cefadroxil) is a new orally active semisynthetic cephalosporin antibiotic with broad-spectrum antibacterial activity. the new compound was evaluated in vitro in comparison with cephalexin. some properties studies such as, antibacterial activity, binding with serum proteins and stability in acid and neutral solution at 37 degrees c for both cephalosporins were similar. in experimental infections of mice, the protective action of bl-s 578 was more effective than cephalexin against staphy ...	1979	33784
studies on some metalloenzymes in aqueous/organic solvents at subzero temperatures [proceedings].		1978	33858
glutamine synthetase regulation, adenylylation state, and strain specificity analyzed by polyacrylamide gel electrophoresis.	we used polyacrylamide gel electrophoresis to examine the regulation and adenylylation states of glutamine synthetases (gss) from escherichia coli (gs(e)) and klebsiella aerogenes (gs(k)). in gels containing sodium dodecyl sulfate (sds), we found that gs(k) had a mobility which differed significantly from that of gs(e). in addition, for both gs(k) and gs(e), adenylylated subunits (gs(k)-adenosine 5'-monophosphate [amp] and gs(e)-amp) had lesser mobilities in sds gels than did the corresponding n ...	1979	33958
peptidoglycan synthesis in cocci and rods of a ph-dependent, morphologically conditional mutant of klebsiella pneumoniae.	mir m7 is a spontaneous morphologically conditional mutant of klebsiella pneumoniae which grows as round cells (cocci) at ph 7 and as normal rods at ph 5.8. we studied the rates of peptidoglycan synthesis of cocci and rods growing at ph values of 7 and 5.8, respectively. it was found that exponentially growing cocci produced a reduced amount of peptidoglycan per cell, compared with rods. moreover, a shift of cocci to the permissive ph (5.8) caused an increase in the rate of peptidoglycan synthes ...	1979	33960
[modern data on the antibiotic sensitivity of the microflora that is an etiological factor in ent diseases. ii. the antibiotic sensitivity of pneumococci, meningococci, influenza bacilli, klebsiella, enterobacteria, pseudomonas aeruginosa and proteus].		1979	34945
enzymes of agmatine degradation and the control of their synthesis in klebsiella aerogenes.	the degradation of agmatine to succinate by klebsiella aerogenes occurs in five steps. the enzyme catalyzing the first step, agmatinase, is induced by agmatine. the enzymes catalyzing the second and third steps, putrescine aminotransferase and 4-aminobutyraldehyde dehydrogenase, are induced by putrescine and also by their product, 4-aminobutyrate. the enzymes catalyzing the fourth and fifth steps, 4-aminobutyrate aminotransferase and succinate semialdehyde dehydrogenase, are induced by 4-aminobu ...	1979	35512
relation between the adenylylation state of glutamine synthetase and the expression of other genes involved in nitrogen metabolism.	we have partially characterized the biochemical parameters of glutamine synthetase from klebsiella pneumoniae and have shown that the differential affinity of adenylylated and unadenylylated glutamine synthetase for adenosine diphosphate provides a convenient means of determining the adenylylation state. using this assay procedure, we examined the relationship between the adenylylation state and the expression of other genes involved in nitrogen assimilation. we observed no correlation between t ...	1979	35515
cefuroxime: a review of its antibacterial activity, pharmacological properties and therapeutic use.	cefuroxime is a new semisynthetic cephalosporin for parenteral administration. it is resistant to destruction by beta-lactamases produced by staphylococci and most gram-negative aerobic bacteria and is active against many bacteria resistant to cephalothin. cefuroxime is the most active of the cephalosporins against gonococci and haemophilus influenzae particularly against beta-lactamase producing strains. given by intramuscular or intravenous injection cefuroxime is effective against a wide vari ...	1979	37064
intradermal responses to common bacterial antigens: influences of sex and clinical condition.	intradermal responses to antigens of 12 common bacteria were evaluated in 218 dermatologic and 155 respiratory patients. women responded more frequently than men and respiratory patients responded significantly more frequently than dermatologic patients to certain antigens. interpretation of interspecies associations of responses suggested that certain organisms may be key species in the development of bacterial hypersensitivity.	1979	37788
ps-5, a new beta-lactam antibiotic. ii. antimicrobial activity.	ps-5, a new beta-lactam antibiotic, has relatively potent antimicrobial activity against gram-positive and gram-negative bacteria, especially the enterobacter groups, serratia marcescens, the proteus groups and klebsiella pneumoniae. the activity of ps-5 against many beta-lactamase-producing organisms is greater than that of cefoxitin or cefazolin. ps-5 has good therapeutic activity in mice infected with staphylococcus aureus smith or enterobacter cloacae 45.	1979	38238
detection of experimental bacteremia and fungemia by examination of buffy coat prepared by a micromethod.	rabbits received intravenous injections of bacteria or fungi, and a comparison was made of the abilities of broth cultures, plating after dilution either in saline solution or in distilled water containing triton x-100, and buffy coat examinations to detect the organisms in heart blood. the most sensitive method was broth culture. by microscopy or subculture of buffy coat cells prepared by centrifugation of blood in microhematocrit tubes, organisms were rapidly and regularly detected when their ...	1979	38662
distinctive properties of glutamine synthetase from the cyanobacterium anacystis nidulans.	the intracellular levels of glutamine synthetase (gs) in anacystis nidulans grown under different conditions were determined using a whole-cell assay. nitrate-grown cells have 64% more gs than cells grown in ammonium sulfate. nitrogen starvation does not affect gs levels appreciably. incubation of nitrate-grown cells with ammonium sulfate does not change the ratio of gamma-glutamyl transferase activities stimulated by mg2+ and mn2+ ions. an in vitro test of adenylylation indicates that algae do ...	1979	38892
the role of bacterial interference in the increased prevalence of oropharyngeal gram-negative bacilli among alcoholics and diabetics.	the oral flora of alcoholics, diabetics, and normal control subjects were compared using an agar overlay technique to determine whether the increased prevalence of oropharyngeal gram-negative bacilli among alcoholics and diabetics exists because patients with these diseases have decreased numbers of normal inhibitory bacteria in the oropharynx. alcoholics generally had slightly lower concentrations of inhibitory bacteria than control subjects, and diabetics had somewhat higher concentrations tha ...	1979	39482
microbiological activity of cefazedone as compared to cefazolin and cephalothin.	in a comparison of the antibacterial activity of (6r,7r)-7-(2-[3,5-dichloro-4-oxo-1(4h)-pyridyl]-acetamido)-3-([5-methyl-1,3,4-thiadiazol-2-yl)-thio]methyl)-8-oxo-5-thia-1-azabicyclo[4,2,0]oct-2-ene-2-carboxylic acid (cefazedone, refosporen) as compared to other cephalosporins it can be seen that cefazedone possesses good activity against gram-positive bacteria; as regards activity against enterobacteriaceae, in particular strains of e. coli, klebsiella species and proteus mirabilis, they fall w ...	1979	39575
evidence for pili-mediated adherence of klebsiella pneumoniae to rat bladder epithelial cells in vitro.	the possible role of pili in the pathogenesis of urinary tract infections caused by klebsiella pneumoniae was studied in an in vitro mixture of a phosphate-buffered saline suspension of rat bladder epithelial cells and phosphate-buffered saline-washed k. pneumoniae. nonpiliated and piliated populations derived from a single k. pneumoniae strain were obtained by controlling the total time of growth in broth medium. the piliated phase demonstrated a significant increase in adherence when compared ...	1979	39895
polymicrobial bacteremia.	of 26,961 blood cultures taken during an 18-month period at the cincinnati general hospital, 1,715 (6%) were positive. ninety-four patients had blood cultures containing more than one organism. although aerobic and anaerobic streptococci were the most frequently isolated bacteria, a variety of microorganisms, including staphylococcus aureus and the klebsiella-enterobacter-serratia group, was isolated in different combinations depending on the underlying disease. neurological illness, malignant n ...	1979	40048
the interaction between microorganisms and their growth environment [proceedings].		1979	40531
glutamine synthetase mutations which affect expression of nitrogen fixation genes in klebsiella pneumoniae.	previous studies have implicated glutamine synthetase (l-glutamate:ammonia ligase [adenosine diphosphate for-ing], ec 6.6.1.2) as a major controlling element of the nitrogen fixation (nif) genes in klebsiella pneumoniae. we report here the isolation of a new class of k. pneumoniae mutants which exhibit altered patterns of nif and hut (histidine utlization) regulation. the expression of nif in these mutants, which were isolated as gln+ (glutamine nonrequiring) revertants of a particular glna muta ...	1979	40960
interferon and cytotoxic factor (cytotoxin) released in the blood of mice infected with mycobacterium bovis bcg. i. enhanced production of interferon and appearance of cytotoxin stimulated by capsular polysaccharide of klebsiella pneumoniae or bacterial lipopolysaccharide.	interferon production stimulated by the active substance (neutral fraction) of the capsular polysaccharide of klebsiella pneumoniae (neutral cps-k) in bcg-infected mice was compared with that by bacterial lipopolysaccharide (lps). prior infection with bcg increased the responsiveness of mice to the lethal effect of neutral cps-k as well as to that of lps. associated with this, bcg-infected mice showed a markedly enhanced ability to produce interferon after stimulation not only by lps but also by ...	1979	41163
resistance of gram-negative bacteria to antibiotics in large calf agglomerations.	the antibiotic resistance of e. coli, citrobacter, enterobacter-klebsiella and pseudomonas aeruginosa strains isolated from calves was tested. a high proportion of multiresistance was found even in e. coli strains isolated from newborn calves. gram-negative bacteria isolated from animals in three large calfhouses were almost 100% resistant to ampicillin, tetracyclines and sulphonamides. multiresistance was general and varied from 5 to 12 antibiotics among different strains. initial high sensitiv ...	1979	41425
[a clinical observation of cefuroxime in pediatric field (author's transl)].	cefuroxime, a new synthetic cephalosporin, was administered to 10 pediatric patients (6 with respiratory tract infection, 2 with urinary tract infection, 1 with sepsis of e. coli and 1 with enterocolitis). the clinical result was good and excellent in all the 10 cases. no side effect was observed in any of them.	1979	41955
evolution of enzyme structure.	three-dimensional structures of enzymes offer evidence about their evolution. there are clear examples of divergent families (e.g. mammalian serine proteases) and convergence (e.g. chymotrypsin and subtilisin). topological similarities in dehydrogenases may reflect an ancient divergence or merely chemical constraints on protein architectures. further experimental evidence is desirable to back up arguments based on molecular morphology. by growing microorganisms on novel foodstuffs in a chemostat ...	1979	42054
[acute hemorrhagic syndrome due to acquired deficiency of factors ii, vii and x. (considerations on a case)].		1979	44762
[comparison of four recently introduced cephalosporins with respect to probability of resistance in multiresistant strains of escherichia coli, proteus mirabilis and klebsiella spec. (author's transl)].	strains with the same number of resistances were arranged in so-called resistance classes. nine classes of resistance (0 to greater to or equal to 8) were formed by means of ten standard chemotherapeutics; the four new cephalosporins were excluded. for every resistance class frequency of cephalosporinresistance was described as coefficient ranging from 0 to 1 (fig. 1). in cephalothin the coefficients were markedly rising only in 6 (7)-fold resistant strains of the species examined. similar but s ...	1979	44785
[the distribution of minimal inhibitory concentrations of recently introduced cephalosporins in multiresistant strains of escherichia coli, proteus mirabilis and klebsiella spec. as revealed by zone sizes of a standardized agar diffusion test (author's transl)].	strains of a species were divided into two groups according to the number of resistances (less than or equal to 4, greater than or equal to 5) using 10 standard chemotherapeutics regularly examined, the new cephalosporins not being among them. these groups of less than or equal to 4- and greater than or equal to 5-fold resistant strains were compared for each cephalosporin tested (fig. 1). the most different distributions of zone diameters (of both groups) were seen in cephalothin, whereas in ce ...	1979	44786
antigenic variation in klebsiella.	the serological reactions of klebsiella strains repeatedly isolated from four patients were examined. variations in capsular antigens of strains from the same patient were mainly restricted to slight changes in the titre of quellung reactions and occasionally differences in cross-reactions were noted. in one case a strain reacted more strongly with a heterologous antiserum than with homologous antiserum after it had been resident in the bowel of a patient for five weeks. no significant antigenic ...	1979	44943
incompatibility of r plasmids from clinical material.	thirteen r plasmids derived from strains of e enterobacteriaceae isolated from clinical material have been characterized. they belonged to many incompatibility groups and differ widely respecting other phenotypic characteristics, even if they come from bacteria isolated from a small geographic area.	1979	44944
[in vitro activity of cefaclor (author's transl)].	a comparative study was conducted on the in vitro activity of cefaclor and other oral cephalosporins against a large number of freshly isolated clinical strains of gram-negative and gram-positive bacteria. the activity of cefaclor against gram-positive pathogens is very similar to that of cephalexin. the action of cefaclor against streptococcus pneumoniae is superior. cefaclor is the most active antibiotic against strains of haemophilus influenzae, and is also more active than cephalexin and cep ...	1979	45087
emergence of antibiotic resistance in hospitals, 1935-1975.	a limited review of the changes in susceptibility of common bacterial pathogens to available antibacterial agents is presented. significant developments in recent years include the following: (1) the emergence of streptococcus pneumoniae with decreased resistance to penicillin and of some strains resistant to several antibiotics; (2) a decline in prevalence of multi-drug-resistant staphylococcus aureus after 1960 following their increasing prevalence in the preceding years (these changes were me ...	1979	45521
[microbiological characteristics of pneumonia in acute leukosis].		1975	45963
controlled trial of therapy in covert bacteriuria of childhood.	sixty-three girls with covert bacteriuria were included in a controlled trial of therapy. recurrent infection in the treated group was common and was not significantly different from the rate of persistent infection in the untreated control group. two children in each group developed clinical pyelonephritis; the others have remained healthy and all of them have a normal rate of growth. 2 years after diagnosis three of the thirty-four children in the control group and one of twenty-six children i ...	1975	46511
fine structure and distribution of extracellular polymer surrounding selected aerobic bacteria.	the structure and distribution of extracellular polymer surrounding bacillus circulans, diplococcus (streptococcus) pneumoniae, streptococcus salivarius, staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, herella vaginacola (acinetobacter calcoaceticus), and agrobacterium tumefaciens were studied by electron microscopy. a modified ruthenium red staining procedure was used to examine the fine structure of capsule and slime. freeze-etching and critical-point drying were used to ...	1975	46774
[characteristics of the interaction of klebsiella rhinoscleromatis dna with the sera from patient with systemic lupus erythematos].	in blood sera of patients with systemic red lupus there were observed antibodies, which interacted in reaction of indirect hemagglutination and in complement fixation test with dna of scleromic bacteria and did not react with the other structures of bacterial cell: with buaven antigen, detergent and capsular polysaccharides. reaction of indirect hemagglutination of scleromic dna with the sera was inhibited by dna preparations of animal origin. the serological activity of dna from scleromic bacte ...	1975	47210
immunological study of anthranilate synthetase.	an immunological study of anthranilate synthetase (asase) has been initiated using quantitative precipitation, enzyme neutralization, and immunodiffusion methods. cross-reactivity of anthranilate synthetase-anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase (asase-prtase) from escherichia coli, klebsiella aerogenes, and salmonella typhimurium and asase from serratia marcescens and pseudomonas putida was detected with antibodies to ?e. coli trypsin-treated asase. cross-reactivit ...	1975	50316
[bacteriological studies of butirosin sulfate, a new aminoglycoside antibiotic (author's transl)].		1975	51941
practice laboratory bacteriology.		1975	53936
effects of igm and igg antibody in patients with bacteremia due to gram-negative bacilli.	earlier studies, which indicated that high titers of o-specific antibody to the patient's infecting organism in acute-phase serum specimens were not associated with a decrease in the frequency of subsequent shock and death in bacteremia due to gram-negative bacilli, were reexamined for evaluation of the protective activity of specific igg and igm antibody. titers of hemagglutination antibody and levels of igm, determined by indirect immunofluorescent staining of the patient's infecting organism, ...	1976	54397
diminished effect of gentamicin under anaerobic or hypercapnic conditions.	the in-vitro activity of gentamicin, judged by m.i.c. determinations, was much reduced when a normal aerobic atmosphere was replaced either by air +4% co2 or by anaerobic conditions. the phenomenon was greatest for staphylococcus aureus, where a decrease in activity of up to 20-fold was found. for escherichia coli, klebsiella aerogenes, enterobacter spp., and proteus spp. the factor of decrease was between 15-fold and 2-5-fold. changes in medium ph, as a result of bacterial growth, can explain t ...	1976	55718
cellular aspects of non-specific stimulation of antibody production by capsular polysaccharide of klebsiella pneumoniae.		1976	57096
[medium with inositol for isolation of klebsiella].		1976	61323
rational choice of penicillins and cephalosporins based on parallel in-vitro and in-vivo tests.	because of the unavailability of strictly comparable data, seven representative penicillins and the five cephalosporins currently used in britain were evaluated in parallel, both in vitro and in vivo. penicillin sensitive and resistant strains of staphylococcus aureus and proteus mirabilis were the main test organisms. minimum bacteriocidal concentrations of cloxacillin, flucloxacillin, cephalothin, and cephazolin in serum were much higher than conventional minimum inhibitory concentrations in t ...	1976	61353
pathogenesis of respiratory klebsiella pneumoniae infection in rats: bacteriological and histological findings and metabolic alterations.	gram-negative bacterial pneumonias have been increasingly important as nosocomial infections. the following model was developed to study the pathogenesis and evaluate therapy of such infections. intranasal instillation of rats with a suspension of 5 x 10(6) klebsiella pneumoniae caused bronchopneumonia with 24 h. bacteria were isolated from the lungs in large numbers (greater than 10(5) colony-forming units [cfu] for at least 13 days after inoculation. thereafter, the viable concentration decrea ...	1977	66201
causative organisms in rhinoscleroma.		1977	66450
necrotising enterocolitis.		1977	67508
klebsiella meningitis treated with intrathecal amikacin.		1977	69095
[effectiveness of intranasal immunization with myxovirus influenzae neuraminidase in mice].	an intranasal immunization with a a/pr8/34-isolated na, protected mice as well as the whole virus and a/hong kong/1/68 virus against a subsequent infection with mice-adaptated a/pr8/34 strain.	1977	69501
a polyvalent human gamma-globulin immune to pseudomonas aeruginosa: passive protection of mice against lethal infection.	as a means to development of guidelines for therapeutic application to human disease, preparations of human polyvalent gamma-globulin immune to pseudomonas aeruginosa (pg) were studied in acute infections in mice. pg was highly effective in controlling lethal infections induced in mice by the major immunotypes of p. aeruginosa; greater than or equal to 10 microgram of of gamma-globulin per mouse protected against challenge with less than or equal to 10(6) 50% lethal doses of p. aeruginosa. pg wa ...	1977	70492
gentamicin-resistant klebsiella aerogenes in a urological ward.	a gentamicin-resistant strain of klebsiella aerogenes was isolated from the urine of 17 patients out of 237 admitted to a male urological ward between jan. 21 and may 9, 1977. the factors most frequently associated with k. aerogenes in the urine were catheterisation and antibiotic therapy. often the epidemic strain (type k16) was found not only in the patients' faeces but also on more remote skin sites such as hands, knees, groins, and the umbilicus. resistance to gentamicin and many other antib ...	1977	70654
[experimental sensitizing activity of neisseria perflava].	in experiments conducted on guinea pigs the sensitizing activity of neisseria perflava isolated from the mucous membranes of the bronchi of patients with infectious asthma was studied. a possibility of reproducing active skin anaphylaxis after ovary and of the contraction-test of the tracheal-chain by the neisseria antigens was shown. neisseria perflava was found to possess a greater sensitizing activity than staphylococcus aureus and klebsiella pneumoniae inhabiting the bronchi of patients with ...	1977	71172
trimethoprim-resistant coliforms.		1977	71593
immunological study of the regulation of cellular arylsulfatase synthesis in klebsiella aerogenes.	regulation of cellular arylsulfatase synthesis in klebsiella aerogenes was analyzed by immunological techniques. antibody directed against the purified arylsulfatase from k. aerogenes w70 was obtained from rabbits and characterized by immunoelectrophoresis, double-diffusion, quantitative precipitation, and enzyme neutralization tests. arylsulfatase was located in the periplasmic space when the wild-type strain was cultured with methionine or with inorganic sulfate plus tyramine, but not with ino ...	1977	72063
[detection among "e. aerogenes" strains of capsular antigens related to those of "klebsiella". interest of growth in metahydroxybenzoate to differenciate "e. aerogenes" and "k. pneumoniae" (author's transl)].	forty-three strains of e. aerogenes isolated chiefly in morocco and france have been studied. thirty-five strains (81%) are surrounded with a thin capsule, antigenically related to klebsiella capsular antigens: k4 (2 strains), k4, 59 (1 strain), k11 (2 strains), k26 (7 strains), k42 (5 strains), k59 (3 strains), k68 (14 strains). one strain is capsulated but not typable with klebsiella capsular antisera. e. aerogenes and klebsiella capsular antigens are not identical but share common fractions y ...	1977	72514
[determination of the capsular antigen of klebsiella rhinoscleromatis by the methods of precipitation in agar and counterimmunoelectrophoresis].		1977	72860
enterobacter, b27, and ankylosing spondylitis.		1978	78003
transfusion of polymorphonuclear neutrophils in a premature infant with klebsiella sepsis.		1978	78284
trimethoprim-resistant klebsiella aerogenes.		1978	78356
synthetic disaccharide-protein antigen for production of specific o2 antiserum for immunofluorescence diagnosis of salmonella.	antisera from rabbits immunized with the synthetic disaccharide paratose 1 leads to alpha 3 mannose, representive of salmonella o-antigen 2, covalently linked to bovine serum albumin (bsa), were used in indirect immunofluorescence studies for the identification of salmonella serogroup a (o-antigen 1,2,12) bacteria. among 1311 enteric bacteria tested, 497 were salmonella. the anti-paratose 1 leads to alpha 3 mannose-bsa serum identified correctly all the 63 serogroup a strains tested. no positive ...	1978	78659
degradation of the polysaccharide component of gonococcal lipopolysaccharide by gonococcal and meningococcal sonic extracts.	an extract made from the supernatant of neisseria gonorrhoeae gc2 strain 1291 degraded the gc2 polysaccharide antigen. chemical analysis of this polysaccharide indicated it contains glucose, galactose, glucosamine, galactosamine, glucosamine-6-phosphate, heptose, 2-keto-3-deoxyotonate, and ethanolamine and is the polysaccharide component of gonococcal lipopolysaccharide. degradation of the polysaccharide by sonic extracts resulted either in complete loss of antigenicity and immunogenicity or in ...	1978	78894
enterotoxigenicity of colonising coliform bacteria in tropical sprue and blind-loop syndrome.	the enterotoxigenicity of strains of klebsiella pneumoniae, enterobacter cloacae, and escherichia coli, which represented the predominant coliform species isolated from the jejunum of 12 patients with tropical sprue and 5 with the blind-loop syndrome, was quantitatively assessed in terms of the ability of toxin preparations to induce water secretion as assayed by in-vivo perfusion in the rat jejunum. all 12 patients with sprue harboured 1 or more highly toxigenic strains--14 of the 16 strains is ...	1978	79710
epidemiology of endemic goitre in western colombia.	this paper reports on recent epidemiological observations in western colombia, which further demonstrate the presence of naturally-occurring goitrogens contaminating water supplies in areas where goitre persists despite prolonged and continuous iodine supplementation. 'prospective' and 'cross-sectional' studies in 41 localities where the populations have been on a uniform and adequate iodine supplementation for the last 10-20 years indicate that, in the endemia of western colombia, environmental ...	1978	80287
further studies on amplification of cell-associated immunological memory by secondary antigenic stimulus.		1978	80739
stomach as source of bacteria colonising respiratory tract during artificial ventilation.	10 adults had intermittent positive-pressure ventilation for 3--34 days. all 10 had paralytic ileus. microbial overgrowth in the stomach was found in 9 patients (bacterial in 7 and fungal in 2); gram-negative bacteria predominated. the trachea invariably became colonised by bacteria, mainly gram-negative organisms. in 3 instances the gram-negative bacteria were found in the stomach before they appeared in the tracheal aspirate, and in 1 case the pathogen originated in the faeces.	1978	81991
recognition sequence of restriction endonuclease kpni from klebsiella pneumoniae.	we have determined the recognition sequence of the restriction endonuclease kpni, previously isolated from klebsiella pneumoniae. the enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. the specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of kpni generated restriction fragments of simian-virus 40 (sv40) dna (1 site), adenovir ...	1978	82935
evidence that l-rhamnose is the antigenic determinant of hyporesponsiveness of balb/c mice to klebsiella pneumoniae type 47.	the genetic polymorphism in early, immunoglobulin m, responsiveness to the klebsiella pneumoniae type 47 polysaccharide (k47-ps) is determinant specific, and l-rhamnose is probably the determinant against which differential responsiveness is expressed. this is inferred from tests of the response to the cross-reacting streptococcus pneumoniae type 23 and non-cross-reacting pneumococcal polysaccharides of known chemical construction. b10.2/sn new-line mice are high responders and balb/c mice are l ...	1978	83296
hla-b27, klebsiella, and ankylosing spondylitis.		1979	85952
septic arthritis due to klebsiella in a b27-positive patient.		1979	85953
klebsiella cross-infection with capsular serotypes 68 and 21.		1979	86083
immunochemical properties of oligosaccharide-protein conjugates with klebsiella-k2 specificity. i. specificity and crossreactivity of anti-conjugate versus anti-bacterial antibodies.		1979	86152
counterimmunoelectrophoresis of blood cultures. temporal relationship of positive gram stain to positive counterimmunoelectrophoresis.	counterimmunoelectrophoresis (cie), gram staining, and quantitative measurements were performed on simulated blood cultures at hourly intervals after inoculation with streptococcus pneumoniae, klebsiella pneumoniae, or haemophilus influenzae. the cie became positive either at the same time as the gram stain or within the ensuing five hours. in no case was cie positive when the gram stain was negative. the numbers of colony-forming units milliliter necessary for a positive cie result varied with ...	1979	86293
counterimmunoelectrophoresis for rapid identification of blood-culture isolates.	a total of 303 blood cultures that were positive by examination of gram-stained smears were tested immediately by counterimmunoelectrophoresis for detection of bacterial antigens. antigen was detected in all 82 blood cultures containing streptococcus pneumoniae and 11 of 22 with klebsiella pneumoniae, two of two with haemophilus influenzae, and one of one with neisseria meningiditis. false-positive cross-reactions in 265 tests occurred only with pneumococcal omniserum in two cases of nongroupabl ...	1979	86294
[simplified and accelerated methods of determining the generic affiliation of bacteria of the intestinal bacilli group].		1979	86647
klebsiella and ankylosing spondylitis--molecular mimicry?		1979	86725
the effect of alpha-fetoprotein on the immune response. iv. effect of treatment with alpha-fetoprotein on mice infected with bacteria in various experimental conditions.		1978	87184
use of intraoperative gram stain during cholecystectomy.		1979	88186
meningitis in the elderly.		1979	88602
control of hospital epidemic of gentamicin-resistant klebsiella aerogenes.		1979	89322
the strategy of infection as a criterion for phylogenetic relationships of non-coli phages morphologically similar to phage t7.	five phages which are morphologically similar to coliphage t7 but attack other host bacteria have been compared to t7 and to its relative, t3, by the following criteria: (a) cross-reactivity with antisera against t7 and t3, (b) dna base sequence homologies, as determined by the c0t technique, (c) synthesis of two phage-coded enzymes: rna polymerase and samase, (d) patterns of phage-directed protein synthesis, as determined by sds-polyacrylamide gel electrophoresis of phage coat subunits. as judg ...	1979	90110
[method of differentiating k. ozaenae and k. rhinoscleromatis by means of a biotest].		1979	92603
antigenic similarity of morphologic type ciii1 bacteriophages.	the aim of the study was to demonstrate antigenic differentiation among bacteriophages belonging to the same morphologic type--ciii1 according to krzywy and slopek or a2 according to ackermann. twenty-six bacteriophages which multiplied on various strains of bacteria of the genera escherichia, shigella and klebsiella, were studied. serologic tests were done by the quantitative complement fixation test. immune sera against 5 phi e. coli. d8 sh. flexneri, g35 sh. sonnei and kl7 klebsiella bacterio ...	1979	92976
quantitation of skin bacteria: lethality of the wash solution used to remove bacteria.	a widely used technique for the quantitative removal of bacteria from the skin uses a detergent, triton x-100 (p, t-octylphenoxynonaethoxyethanol), to remove and suspend the bacteria. we determined the half-life for the survival of five common skin bacteria suspended in the solution. the shortest-lived was streptococcus pyogenes with a half-life of 0.9 hours. all of the others (propionibacterium acnes, staphylococcus epidermidis, staphylococcus aureus, and klebsiella pneumoniae) had half-lives o ...	1979	93373
does alpha-fetoprotein influence the immune response?		1978	94254
identification of bacteroides fragilis by indirect immunofluorescence.	rabbit antiserum against live or heat-killed bacteroides fragilis nctc 9343 bacteria was titrated against hot phenol water-extracted polysaccharide antigens from five different species of the 'b. fragilis group' of bacteria using an enzyme immunoassay and shown to be specific for the b. fragilis nctc 9343 polysaccharide. when the antiserum was used in indirect immunofluorescence, 97.1% of 244 b. fragilis strains were correctly identified. only 8 of the other 312 bacteroides strains were stained ...	1979	94651
role of interferon in mice in protection against influenza a virus by bacterial ribosomes together with membranal glycoproteins of klebsiella pneumoniae as adjuvant.	nonspecific protection against infectious aerosols of influenza a virus was obtained in swiss mice after vaccination by aerosols of bacterial ribosomes together with membranal glycoproteins extracted from klebsiella pneumoniae as the adjuvant. it was shown that repeated stimulant aerosols were necessary to obtain this protection. routine estimation of serum interferon levels after administration of the association of ribosomes plus membranal glycoproteins to the animals by aerosol or intravenous ...	1979	94908
[interferon in the anti-influenza protection provided by a mixtures of bacterial ribosomes and a membrane fraction of k. pneumoniae applied as an aerosol].		1979	94959
mezlocillin: a new acyl ureidopenicillin. antimicrobial activity and combination effects with four aminoglycoside antibiotics.	mezlocillin (baypen), carbenicillin and mezlocillin combined with gentamicin, sisomicin, tobramycin and amikacin, respectively, were tested in vitro against 325 fresh clinical isolates of pathogenic gram-negative bacteria using the tube dilution procedure. the new acyl ureidopenicillin has a greater antimicrobial activity than carbenicillin against nearly all species tested. the comparison of the effectiveness of the mezlocillin-aminoglycoside combinations demonstrates the superiority of mezloci ...	1978	95925
bacterial sensitivity to minocycline (minocin) of clinical isolations.		1977	95945
molybdenum independence of nitrogenase component synthesis in the non-heterocystous cyanobacterium plectonema.	the cyanobacterium plectonema boryanum (iu 594-utex 594) fixes n2 only in the absence of combined n and of o2. we induced nitrogenase by transfer to anaerobic n-free medium and studied the effect of mo starvation on nitrogenase activity and synthesis. activity was first detected within 3 h after transfer by the acetylene reduction assay in controls, increasing for at least 25 h. cells grown on nitrate and mo and then transferred to n-free, mo-free medium produced 8% of the control nitrogenase ac ...	1978	96092
a survey of bacterial infections in the surgical intensive care unit.		1978	96533
activity of azlocillin and mezlocillin against gram-negative organisms: comparison with other penicillins.	the activities of azlocillin and mezlocillin were compared with those of carbenicillin, ticarcillin, and pirbenicillin against a wide range of gram-negative organisms. the two new drugs were considerably more active than carbenicillin against klebsiella species and escherichia coli. carbenicillin was twice as active against proteus mirabilis as mezlocillin and four times as active as azlocillin. against pseudomonas aeruginosa, azlocillin was eight times as active as carbenicillin. azlocillin and ...	1978	96726
biological nitrogen fixation in the terrestrial environment of a high arctic ecosystem (truelove lowland, devon island, n.w.t.).	arranged in descending order of nitrogen-fixing (acetylene-reducing) potential the sites examined were mesic meadow and peat polygon troughs (equal rank), transition zone between mesic meadow and gravel ridge, gravel ridge, polar dessert, and peat polygon tops. the dominant nitrogen-fixing microorganisms, as in other arctic areas, were blue-green bacteria, especially those epiphytic on arctic mosses. the epiphytic association exhibited an optimum temperature for fixation of 20 degrees c. other b ...	1978	96927
relationship of method of administration to respiratory virulence of klebsiella pneumoniae for mice and squirrel monkeys.	klebsiella pneumoniae given by aerosol was significantly less virulent in mice and monkeys than when given by intranasal (mice) or intratracheal (monkeys) instillation.	1978	97232
f'-plasmid transfer from escherichia coli to pseudomonas fluorescens.	various f' plasmids of escherichia coli k-12 could be transferred into mutants of the soil strain 6.2, classified herein as a pseudomonas fluorescens biotype iv. this strain was previously found to receive flac plasmid (n. datta and r.w. hedges, j. gen microbiol. 70:453-460, 1972). ilv, leu, met, arg, and his auxotrophs were complemented by plasmids carrying isofunctional genes; trp mutants were not complemented or were very poorly complemented. the frequency of transfer was 10(-5). subsequent t ...	1978	97267
gentamicin-resistant gram-negative bacilli in hospital patients. part i. preliminary epidemiological assessment.	gentamicin-resistant gram-negative bacilli (grnb) have been isolated from specimens received from 23 hospitals in and around johannesburg. most isolates are fermentative in nature. a pilot survey among inpatients at the johannesburg hospital revealed an intestinal carriage prevalence of 14,3%. a prospective study of intestinal acquisition showed that grnb are acquired in hospital, and that colonization is associated with prior antibacterial therapy. analysis of clinical specimens received from t ...	1978	97791
immunological study of glutamate synthase and its subunits.		1978	98351
in vitro susceptibility of gentamicin and/or tobramycin resistant gram-negative bacilli to seven aminoglycosides.	the in vitro activity of gentamicin, tobramycin, sisomicin, netilmicin, amikacin, kanamycin and streptomycin was tested simultaneously by the agar dilution method against 584 clinical isolates of gram-negative bacilli that were resistant to gentamicin and/or tobramycin. about half of the gentamicin-resistant pseudomonas were susceptible to tobramycin but cross-resistance was virtually complete between gentamicin and tobramycin for enterobacteriaceae. sisomicin was much more active than gentamici ...	1978	98451
catheter infection factors affecting total parenteral nutrition.	during the five-year interval from january 1, 1971 to january 1, 1976 118 seriously ill adults received 2916 patient days of tpn therapy with an average infection rate of 7.6%. all patients received the benefit of a well-defined tpn catheter care protocol which emphasized regular (every 48 hours) catheter dressing changes. the lowest risk of infection, 2.7%, was seen in 73 patients who received an amino acid-glucose solution through a silicone elastomer catheter protected by an iodophor dressing ...	1978	99066
antagonistic interaction of rifampicin and trimethoprim.		1978	99420
susceptibility of noxythiolin resistant organisms to povidone iodine.		1978	99421
[the antibacterial activity of diphenhydramine (author's transl)].	diphenhydramine (n,n-dimethyl-2-diphenyl-methoxy-ethylamine) [n,n-dimethyl-2-(diphenylmethoxy)-ethylamin] (dph) is a well-known local anaesthetic and an antagonist of both acetylcholine and histamine. we tested its antibacterial efficacy by means of three different investigation methods, i.e. microcalorimetry, continuous density measurement and the microdilution test to find out the mic of this compound against various bacteria. dph inhibits the growth of e. coli and klebsiella strains at a conc ...	1978	99881