Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| baygo: bayesian analysis of ontology term enrichment in microarray data. | the search for enriched (aka over-represented or enhanced) ontology terms in a list of genes obtained from microarray experiments is becoming a standard procedure for a system-level analysis. this procedure tries to summarize the information focussing on classification designs such as gene ontology, kegg pathways, and so on, instead of focussing on individual genes. although it is well known in statistics that association and significance are distinct concepts, only the former approach has been ... | 2006 | 16504085 |
| expression, purification, crystallization and preliminary x-ray analysis of yaeq (xac2396) from xanthomonas axonopodis pv. citri. | xanthomonas axonopodis pv. citri yaeq (xac2396) is a member of a family of bacterial proteins conserved in several gram-negative pathogens. here, the cloning, expression, purification and crystallization of the 182-residue (20.6 kda) yaeq protein are described. recombinant yaeq containing selenomethionine was crystallized in space group p2(1) and crystals diffracted to 1.9 a resolution at a synchrotron source. the unit-cell parameters are a = 39.75, b = 91.88, c = 48.03 a, beta = 108.37 degrees. ... | 2005 | 16511077 |
| expression, crystallization and preliminary crystallographic analysis of sufe (xac2355) from xanthomonas axonopodis pv. citri. | xanthomonas axonopodis pv. citri (xac) sufe (xac2355) is a member of a family of bacterial proteins that are conserved in several pathogens and phytopathogens. the escherichia coli suf operon is involved in iron-sulfur cluster biosynthesis under iron-limitation and stress conditions. it has recently been demonstrated that sufe and sufs form a novel two-component cysteine desulfarase in which sufs catalyses the conversion of l-cysteine to l-alanine, forming a protein-bound persulfide intermediate ... | 2006 | 16511319 |
| crystallization, data collection and phasing of the molybdate-binding protein of the phytopathogen xanthomonas axonopodis pv. citri. | xanthomonas axonopodis pv. citri moda protein is the abc periplasmic binding component responsible for the capture of molybdate. the protein was crystallized with sodium molybdate using the hanging-drop vapour-diffusion method in the presence of peg or sulfate. x-ray diffraction data were collected to a maximum resolution of 1.7 a using synchrotron radiation. the crystal belongs to the orthorhombic space group c222(1), with unit-cell parameters a = 68.15, b = 172.14, c = 112.04 a. the crystal st ... | 2006 | 16511325 |
| identification of genomic islands in six plant pathogens. | genomic islands (gis) play important roles in microbial evolution, which are acquired by horizontal gene transfer. in this paper, the gis of six completely sequenced plant pathogens are identified using a windowless method based on z curve representation of dna sequences. consequently, four, eight, four, one, two and four gis are recognized with the length greater than 20-kb in plant pathogens agrobacterium tumefaciens str. c58, rolstonia solanacearum gmi1000, xanthomonas axonopodis pv. citri st ... | 2006 | 16581205 |
| nmr assignment of the outer membrane lipoprotein (omla) from xanthomonas axonopodis pv citri. | 2006 | 16596342 | |
| a study on the molecular mechanism of resistance to amicarthiazol in xanthomonas campestris pv. citri. | three amicarthiazol-resistant mutants (xuv10, xuv20 and xuv40) were obtained by uv induction and used in this study. minimal inhibition concentrations (mics) of amicarthiazol against the growth of mutants and wild-type isolate were 400 and 100 microg ml(-1) respectively. inhibition by amicarthiazol of succinate dehydrogenase (sdh) activities of xanthomonas campestris pv. citri (hasse) dye wild-type isolate (xcc) and three resistant mutants derived from this isolate were assayed using triphenylte ... | 2006 | 16602085 |
| crystallization and preliminary x-ray diffraction analysis of xac1151, a small heat-shock protein from xanthomonas axonopodis pv. citri belonging to the alpha-crystallin family. | the hspa gene (xac1151) from xanthomonas axonopodis pv. citri encodes a protein of 158 amino acids that belongs to the small heat-shock protein (shsp) family of proteins. these proteins function as molecular chaperones by preventing protein aggregation. the protein was crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium phosphate. x-ray diffraction data were collected to 1.65 angstroms resolution using a synchrotron-radiation source. the crystal belongs to the ... | 2006 | 16682772 |
| [study on a bacterial strain bt8 for biocontrol against citrus bacterial canker]. | citrus bacterial canker is an important disease of citrus species in china. the disease severely occurs especially in the coastal area. integrated control system has been used for the control of the disease, in which chemotherapy plays an important role at present. the chemotherapy-dominant control system brought many problems to the environment, such as chemical residua in the products and induction of resistance to fungicide(s) by the pathogen. to solve these problems, an intensive study on bi ... | 2006 | 16736594 |
| the molybdate-binding protein (moda) of the plant pathogen xanthomonas axonopodis pv. citri. | the modabc operon of phytopathogen xanthomonas axonopodis pv. citri (x. citri) encodes a putative abc transporter involved in the uptake of the molybdate and tungstate anions. sequence analyses showed high similarity values of moda orthologs found in x. campestris pv. campestris (x. campestris) and escherichia coli. the x. citri moda gene was cloned in pet28a and the recombinant protein, expressed in the e. coli bl21 (de3) strain, purified by immobilized metal affinity chromatography. the purifi ... | 2006 | 16879982 |
| the hd-gyp domain of rpfg mediates a direct linkage between the rpf quorum-sensing pathway and a subset of diguanylate cyclase proteins in the phytopathogen xanthomonas axonopodis pv citri. | bacteria use extracellular levels of small diffusible autoinducers to estimate local cell-density (quorum-sensing) and to regulate complex physiological processes. the quorum-sensing signal transduction pathway of xanthomonas spp. phytopathogens has special features that distinguish it from that of other pathogens. this pathway consists of rpff, necessary for the production of the unique autoinducer 'diffusible signalling factor' (dsf), and rpfc and rpfg, a two-component system necessary for the ... | 2006 | 17020586 |
| dwarf--a data warehouse system for analyzing protein families. | the emerging field of integrative bioinformatics provides the tools to organize and systematically analyze vast amounts of highly diverse biological data and thus allows to gain a novel understanding of complex biological systems. the data warehouse dwarf applies integrative bioinformatics approaches to the analysis of large protein families. | 2006 | 17094801 |
| expression, purification, and characterization of an aminopeptidase (xac2987) with broad specificity from xanthomonas axonopodis pv. citri. | we report here, the cloning, expression, and purification of a broad specificity aminopeptidase from xanthomonas campestris pv. citri in fusion with a hexa-histidine tag at the n-terminal portion of the protein to facilitate purification. the protein was expressed in the soluble fraction and could be purified in one step by imac, yielding approximately 50mg pure protein per liter of cells. we show that the protein is folded and presents aminopeptidase activity against synthetic substrates. also, ... | 2007 | 17110128 |
| insight in to the phylogeny of polyhydroxyalkanoate biosynthesis: horizontal gene transfer. | polyhydroxyalkanoates (phas) are gaining more and more importance the world over due to their structural diversity and close analogy to plastics. their biodegradability makes them extremely desirable substitutes for synthetic plastics. phas are produced in organisms under certain stress conditions. here, we investigated 253 sequenced (completely and unfinished) genomes for the diversity and phylogenetics of the pha biosynthesis. discrepancies in the phylogenetic trees for phaa, phab and phac gen ... | 2007 | 17113245 |
| identification and characterization of two uvra genes of xanthomonas axonopodis pathovar citri. | two uvra-like genes, designated uvra1 and uvra2, that may be involved in nucleotide excision repair in xanthomonas axonopodis pv. citri (x. a. pv. citri) strain xw47 were characterized. the uvra1 gene was found to be 2,964 bp in length capable of encoding a protein of 987 amino acids. the uvra2 gene was determined to be 2,529 bp with a coding potential of 842 amino acids. these two proteins share 71 and 39% identity, respectively, in amino acid sequence with the uvra protein of escherichia coli. ... | 2007 | 17146667 |
| absence of classical heat shock response in the citrus pathogen xylella fastidiosa. | the fastidious bacterium xylella fastidiosa is associated with important crop diseases worldwide. we have recently shown that x. fastidiosa is a peculiar organism having unusually low values of gene codon bias throughout its genome and, unexpectedly, in the group of the most abundant proteins. here, we hypothesized that the lack of codon usage optimization in x. fastidiosa would incapacitate this organism to undergo quick and massive changes in protein expression as occurs in a classical stress ... | 2007 | 17211542 |
| in planta horizontal transfer of a major pathogenicity effector gene. | xanthomonas citri pv. citri is a clonal group of strains that causes citrus canker disease and appears to have originated in asia. a phylogenetically distinct clonal group that causes identical disease symptoms on susceptible citrus, x. citri pv. aurantifolii, arose more recently in south america. genomes of x. citri pv. aurantifolii strains carry two dna fragments that hybridize to ptha, an x. citri pv. citri gene which encodes a major type iii pathogenicity effector protein that is absolutely ... | 2007 | 17220258 |
| solution structure of apag from xanthomonas axonopodis pv. citri reveals a fibronectin-3 fold. | apag proteins are found in a wide variety of bacterial genomes but their function is as yet unknown. some eukaryotic proteins involved in protein-protein interactions, such as the human polymerase delta-interacting protein (pdip38) and the f box a (fba) proteins, contain apag homology domains. we have used nmr to determine the solution structure of apag protein from the plant pathogen xanthomonas axonopodis pv. citri (apag(xac)) with the aim to shed some light on its molecular function. apag(xac ... | 2007 | 17256769 |
| a ptha homolog from xanthomonas axonopodis pv. citri responsible for host-specific suppression of virulence. | strains of the plant-pathogenic bacterium xanthomonas axonopodis pv. citri are differentiated into two groups with respect to aggressiveness (normal and weak) on citrus grandis cultivars but not on other citrus species such as citrus sinensis. random mutagenesis using the transposon tn5 in x. axonopodis pv. citri strain kc21, which showed weak aggressiveness on a c. grandis cultivar, was used to isolate mutant kc21t46, which regained a normal level of aggressiveness on the cultivar. the gene ina ... | 2007 | 17293422 |
| cloning and characterization of three hypothetical secretion chaperone proteins from xanthomonas axonopodis pv. citri. | xanthomonas axonopodis pv. citri (xac) causes citrus canker in plantations around the world and is of particular significance in brazil where its incidence has risen exponentially over the past decade. approximately one third of the predicted xac open reading frames show no homology, or homology with very low score with that of known sequences. it is believed that xac utilizes secretion systems to transfer virulence proteins into susceptible eukaryotic cells. this process is assisted by secretio ... | 2007 | 17350859 |
| xanthan is not essential for pathogenicity in citrus canker but contributes to xanthomonas epiphytic survival. | xanthan-deficient mutants of xanthomonas axonopodis pv. citri, the bacterium responsible for citrus canker, were generated by deletion and marker exchange of the region encoding the carboxy-terminal end of the first glycosyltransferase, gumd. mutants of gumd did not produce xanthan and remained pathogenic in citrus plants to the same extent as wild-type bacteria. the kinetics of appearance of initial symptoms, areas of plant material affected, and growth of bacteria inside plant tissue throughou ... | 2007 | 17356870 |
| repeat domain diversity of avrbs3-like genes in ralstonia solanacearum strains and association with host preferences in the field. | genes homologous to avrbs3 of xanthomonas were detected in 309 strains of ralstonia solanacearum biovars 3, 4, and 5 but not biovar 1 or 2. a statistically significant association between the originating plant species and internal repeats of the gene was found. sequences of repeats and variation between nearly clonal strains revealed evidence of frequent recombination. | 2007 | 17468277 |
| identification of the flagellar chaperone flgn in the phytopathogen xanthomonas axonopodis pathovar citri by its interaction with hook-associated flgk. | genome annotation of the plant pathogen xanthomonas axonopodis pv. citri (xac), identified flagellar genes in a 15.7 kb gene cluster. however, flgn, a secretion chaperone for hook-associated proteins flgk and flgl, was not identified. we performed extensive screening of the x. axonopodis pv. citri genome with the yeast two-hybrid system to identify a protein with the characteristics of the flagellar chaperone flgn. we found a candidate (xac1990) encoded by an operon for components of the flagell ... | 2007 | 17492271 |
| characterization of the first angiotensin-converting like enzyme in bacteria: ancestor ace is already active. | angiotensin-converting enzyme (ace) is a metallopeptidase that converts angiotensin i into angiotensin ii. ace is crucial in the control of cardiovascular and renal homeostasis and fertility in mammals. in vertebrates, both transmembrane and soluble ace, containing one or two active sites, have been characterized. so far, only soluble, single domain aces from invertebrates have been cloned, and these have been implicated in reproduction in insects. furthermore, an ace-related carboxypeptidase wa ... | 2007 | 17597310 |
| structure of xanthomonas axonopodis pv. citri yaeq reveals a new compact protein fold built around a variation of the pd-(d/e)xk nuclease motif. | the yaeq family of proteins are found in many gram-negative and a few gram-positive bacteria. we have determined the first structure of a member of the yaeq family by x-ray crystallography. comparisons with other structures indicate that yaeq represents a new compact protein fold built around a variation of the pd-(d/e)xk nuclease motif found in type ii endonucleases and enzymes involved in dna replication, repair, and recombination. we show that catalytically important residues in the pd-(d/e)x ... | 2007 | 17623842 |
| all five host-range variants of xanthomonas citri carry one ptha homolog with 17.5 repeats that determines pathogenicity on citrus, but none determine host-range variation. | citrus canker disease is caused by five groups of xanthomonas citri strains that are distinguished primarily by host range: three from asia (a, a*, and a(w)) and two that form a phylogenetically distinct clade and originated in south america (b and c). every x. citri strain carries multiple dna fragments that hybridize with ptha, which is essential for the pathogenicity of wide-host-range x. citri group a strain 3213. dna fragments that hybridized with ptha were cloned from a representative stra ... | 2007 | 17722697 |
| biofilm formation, epiphytic fitness, and canker development in xanthomonas axonopodis pv. citri. | the phytopathogenic bacterium xanthomonas axonopodis pv. citri is responsible for the canker disease affecting citrus plants throughout the world. here, we have evaluated the role of bacterial attachment and biofilm formation in leaf colonization during canker development on lemon leaves. crystal violet staining and confocal laser scanning microscopy analysis of x. axonopodis pv. citri strains expressing the green fluorescent protein were used to evaluate attachment and biofilm formation on abio ... | 2007 | 17918624 |
| computational biology in brazil. | 2007 | 17967046 | |
| genetic diversity of citrus bacterial canker pathogens preserved in herbarium specimens. | citrus bacterial canker (cbc) caused by xanthomonas axonopodis pv. citri (xac) was first documented in india and java in the mid 19th century. since that time, the known distribution of the disease has steadily increased. concurrent with the dispersion of the pathogen, the diversity of described strains continues to increase, with novel strains appearing in saudi arabia, iran, and florida in the last decade. herbarium specimens of infected plants provide an historical record documenting both the ... | 2007 | 17998540 |
| diagnosis of xanthomonas axonopodis pv. citri, causal agent of citrus canker, in commercial fruits by isolation and pcr-based methods. | to show the results of the detection of an eu quarantine organism, xanthomonas axonopodis pv. citri (xac), in citrus fruits imported from countries where this bacterium is present, using an integrated approach that includes isolation, pathogenicity assays and molecular techniques. | 2007 | 18045415 |
| crystallographic structure and substrate-binding interactions of the molybdate-binding protein of the phytopathogen xanthomonas axonopodis pv. citri. | in xanthomonas axonopodis pv. citri (xac or x. citri), the moda gene codes for a periplasmic protein (moda) that is capable of binding molybdate and tungstate as part of the abc-type transporter required for the uptake of micronutrients. in this study, we report the crystallographic structure of the xac moda protein with bound molybdate. the xac moda structure is similar to orthologs with known three-dimensional structures and consists of two nearly symmetrical domains separated by a hinge regio ... | 2008 | 18088604 |
| the solution structure of the outer membrane lipoprotein omla from xanthomonas axonopodis pv. citri reveals a protein fold implicated in protein-protein interaction. | the outer membrane lipoprotein a (omla) belongs to a family of bacterial small lipoproteins widely distributed across the beta and gamma proteobacteria. although the role of numerous bacterial lipoproteins is known, the biological function of omla remains elusive. we found that in the citrus canker pathogen, xanthomonas axonopodis pv. citri (x. citri), omla is coregulated with the ferric uptake regulator (fur) and their expression is enhanced when x. citri is grown on citrus leaves, suggesting t ... | 2008 | 18186471 |
| the copper resistance operon copab from xanthomonas axonopodis pathovar citri: gene inactivation results in copper sensitivity. | xanthomonas axonopodis pv. citri (xac) causes citrus canker and the completion of the xac genome sequence has opened up the possibility of investigating basic cellular mechanisms at the genomic level. copper compounds have been extensively used in agriculture to control plant diseases. the copa and copb genes, identified by annotation of the xac genome, encode homologues of proteins involved in copper resistance. a gene expression assay by northern blotting revealed that copa and copb are expres ... | 2008 | 18227244 |
| [high efficient expression and bio-activity assay of recombinant antibody for citrus bacterial canker disease]. | citrus bacterial canker disease caused by the gram negative bacterium xanthomonas axonopodis pv. citri (xac) is a severe bacterial disease of most commercial citrus species and cultivars around the world. single chain variable fragment (scfv) is artificial construction small molecular antibody produced by genetic engineering which may be used to identify target pathogens and prevent plant diseases including xac. to express scfv against xac and obtain functional scfv, single-chain antibody fragme ... | 2007 | 18271265 |
| structural model and ligand interactions of the xanthomonas axonopodis pv. citri oligopeptide-binding protein. | the oligopeptide-binding protein, oppa, ushers oligopeptide substrates to the membrane-associated oligopeptide permease (opp), a multi-component abc-type transporter involved in the uptake of oligopeptides by several bacterial species. in the present study, we report a structural model and an oligopeptide docking analysis of the oppa protein expressed by xanthomonas axonopodis pv. citri (x. citri), the etiological agent of citrus canker. the x. citri oppa structural model showed a conserved thre ... | 2007 | 18273810 |
| production of the refolded oligopeptide-binding protein (oppa) encoded by the citrus pathogen xanthomonas axonopodis pv. citri. | the oligopeptide-binding protein, oppa, binds and ushers oligopeptide substrates to the membrane-associated oligopeptide permease (opp), a multi-component abc-type transporter involved in the uptake of oligopeptides expressed by several bacterial species. in the present study, we report the cloning, purification, refolding and conformational analysis of a recombinant oppa protein derived from xanthomonas axonopodis pv. citri (x. citri), the etiological agent of citrus canker. the oppa gene was e ... | 2008 | 18273827 |
| in vivo proteome analysis of xanthomonas campestris pv. campestris in the interaction with the host plant brassica oleracea. | the genus xanthomonas is composed of several species that cause severe crop losses around the world. in latin america, one of the most relevant species is xanthomonas campestris pv. campestris, which is responsible for black rot in cruciferous plants. this pathogen causes yield losses in several cultures, including cabbage, cauliflower and broccoli. although the complete structural genome of x. campestris pv. campestris has been elucidated, little is known about the protein expression of this pa ... | 2008 | 18318710 |
| laterally transferred genomic islands in xanthomonadales related to pathogenicity and primary metabolism. | lateral gene transfer (lgt) is considered as one of the drivers in bacterial genome evolution, usually associated with increased fitness and/or changes in behavior, especially if one considers pathogenic vs. non-pathogenic bacterial groups. the genomes of two phytopathogens, xanthomonas campestris pv. campestris and xanthomonas axonopodis pv. citri, were previously inspected for genome islands originating from lgt events, and, in this work, potentially early and late lgt events were identified a ... | 2008 | 18318843 |
| the type iii secretion system of xanthomonas fuscans subsp. fuscans is involved in the phyllosphere colonization process and in transmission to seeds of susceptible beans. | understanding the survival, multiplication, and transmission to seeds of plant pathogenic bacteria is central to study their pathogenesis. we hypothesized that the type iii secretion system (t3ss), encoded by hrp genes, could have a role in host colonization by plant pathogenic bacteria. the seed-borne pathogen xanthomonas fuscans subsp. fuscans causes common bacterial blight of bean (phaseolus vulgaris). directed mutagenesis in strain cfbp4834-r of x. fuscans subsp. fuscans and bacterial popula ... | 2008 | 18326683 |
| detection of mechanical and disease stresses in citrus plants by fluorescence spectroscopy. | we have investigated the detection of mechanical and disease stresses in citrus plants (citrus limonia [l.] osbeck) using laser-induced fluorescence spectroscopy. due to its economic importance we have chosen to investigate the citrus canker disease, which is caused by the xanthomonas axonopodis pv. citri bacteria. mechanical stress was also studied because it plays an important role in the plant's infection by such bacteria. a laser-induced fluorescence spectroscopy system, composed of a spectr ... | 2008 | 18404192 |
| analyses of binding sequences of the two lexa proteins of xanthomonas axonopodis pathovar citri. | xanthomonas axonopodis pv. citri (x. axonopodis pv. citri) possesses two lexa genes, designated lexa1 and lexa2. electrophoretic mobility shift data show that lexa1 binds to both lexa1 and lexa2 promoters, but lexa2 does not bind to the lexa1 promoter, suggesting that lexa1 and lexa2 play different roles in regulating the expression of sos genes. in this study, we have determined that lexa2 binds to a 14-bp dyad-spacer-dyad palindromic sequence, 5'-tgtacaaatgtaca-3', located at nucleotides -41 t ... | 2008 | 18437426 |
| genome sequence and rapid evolution of the rice pathogen xanthomonas oryzae pv. oryzae pxo99a. | xanthomonas oryzae pv. oryzae causes bacterial blight of rice (oryza sativa l.), a major disease that constrains production of this staple crop in many parts of the world. we report here on the complete genome sequence of strain pxo99a and its comparison to two previously sequenced strains, kacc10331 and maff311018, which are highly similar to one another. | 2008 | 18452608 |
| [detection system for xanthomonas axonopodis pv. citri using rolling circle amplification]. | padlock probe was designed based on the sequence of the unique hypothetic protein gene in complete genome of xanthomonas axonopodis pv. citri (xac), and amplification primers ware designed according to the universal linking sequence of padlock probe. detection system of rolling circle amplification (rca) was established and optimized. results show that the system could detect xac and its dna specifically, while other plant pathogens and bacteria attached on the surface of citrus leaves could not ... | 2008 | 18479066 |
| morphological evidence for phages in xylella fastidiosa. | presumptive phage particles associated with xylella fastidiosa strain temecula-1 grown in pw broth were observed by transmission electron microscopy (tem) in ultrathin sections of bacterial cell-containing low speed centrifugation pellets and in partially purified preparations from cscl equilibrium centrifugation density gradients. ultrathin-sectioned cell pellets contained icosahedral particles of about 45 nm in diameter. samples collected from cscl density gradients revealed mostly non-tailed ... | 2008 | 18538030 |
| reaction kinetics of cephalexin synthesizing enzyme from xanthomonas citri. | in enzymatic synthesis of cephalexin from d-alpha-phenylglycine methyl ester (pgm) and 7-amino-3-deacetoxy-cephalosporanic acid (7-adca) using alpha-acylamino-beta-lactam acylhydrolase from xanthomonas citri, it was found that this enzyme catalyzes all three reactions including pgm hydrolysis, cephalexin synthesis, and cephalexin hydrolysis. based on our experimental results, a mechanistic kinetic model for cephalexin synthesizing enzyme system having acyl-enzyme intermediate was proposed. from ... | 1985 | 18553764 |
| screening of high-affinity scfvs from a ribosome displayed library using biacore biosensor. | an experimental protocol was developed to screen high-affinity single-chain fv antibody fragments (scfvs) from a xanthomonas axonopodis pv. citri (xac) immunized ribosome display library using biacore biosensor. the screening methods involved immobilizing antigen [lipopolysaccharides (lps) of xac] on sensor chip hpa and then unpurified expression products of scfvs flowing over the immobilized sensor chip. the affinity-improved scfvs were selected based on dissociation rate constants (k (d)). thi ... | 2009 | 18574567 |
| complete sequence of p07-406, a 24,179-base-pair plasmid harboring the blavim-7 metallo-beta-lactamase gene in a pseudomonas aeruginosa isolate from the united states. | an outbreak involving a pseudomonas aeruginosa strain that was resistant to all tested antimicrobials except polymyxin b occurred in a hospital in houston, tx. previous studies on this strain showed that it possesses a novel mobile metallo-beta-lactamase (mbl) gene, designated bla(vim-7), located on a plasmid (p07-406). here, we report the complete sequence, annotation, and functional characterization of this plasmid. p07-406 is 24,179 bp in length, and 29 open reading frames were identified rel ... | 2008 | 18591274 |
| enhancement effect of polyethylene glycol on enzymatic synthesis of cephalexin. | in an enzymatic synthesis of cephalexin (cex) using an acylase from xanthomonas citri, the effect of polyethylene glycol (peg) on the synthetic reaction of 7-amino-3-deacetoxycephalosporanic acid (7-adca) and d-alpha-phenyl-glycine methyl ester (pgm) to cex was investigated. the addition of peg (mw 300-20,000) increased the yield significantly. this yield enhancement effect tended to increase with the increasing molecular weight of peg. addition of peg to the reaction system did not affect both ... | 1993 | 18609601 |
| enhancement effect of water activity on enzymatic synthesis of cephalexin. | the effect of water activity (a(w)) of the reaction medium on the enzymatic synthesis of cephalexin (cex) from 7-amino-3-deacetoxycephalosporanic acid (7-adca) and d-alpha-phenylglycine methyl ester (pgm) was investigated using the alpha-amino acid ester hydrolase enzyme from xanthomonas citri. it was found that the synthetic activity of the enzyme and the conversion yield were markedly improved when the a(w) of the reaction medium was lowered to about 0.97. the water activity depressing agents ... | 1993 | 18613126 |
| the lysogenic cycle of the filamentous phage cflt from xanthomonas campestris pv. citri. | a phage, cflt, forming turbid plaques, was isolated from xanthomonas campestris pv. citri. after infection, infected sensitive cells become immune to cflt and produce very few phages. these properties were genetically rather stable. the phage was purified and shown to be filamentous with a size of 1157 +/- 73 nm. the genome size is about 7.62 kb. the phage does not affect the growth of host bacteria. under natural cultivation conditions cflt-lysogenized cells could be induced spontaneously to gi ... | 1987 | 18644553 |
| neolysogenization of xanthomonas campestris pv. citri infected with filamentous phage cf16. | all previously described filamentous bacteriophages are capable of persistent infection while their dna replicates as an episome in the host cell. filamentous phage cf16 undergoes an infectious cycle different from other filamentous phages reported heretofore. upon initial infection with cf16, infective centers are formed, each of which produces a large number of phage particles. as the infectious cycle progresses, the phage particles released and infective centers formed per carrier cell decrea ... | 1987 | 18644554 |
| ligation-mediated pcr, a fast and reliable technique for insertion sequence-based typing of xanthomonas citri pv. citri. | asiatic citrus canker, caused by xanthomonas citri pv. citri, is a major disease threatening citrus crops throughout the world. the most common methods for strain differentiation of this pathogen are repetitive element sequence-based pcr (rep-pcr) and pulsed field gel electrophoresis (pfge), using rare-cutting restriction enzyme analysis. we developed a ligation-mediated pcr targeting three insertion sequences (is-lm-pcr) present as several copies in the genome of the fully sequenced strain 306 ... | 2008 | 18771421 |
| survival of xanthomonas axonopodis pv. citri in leaf lesions under tropical environmental conditions and simulated splash dispersal of inoculum. | abstract asiatic citrus canker (acc) is a severe disease of several citrus species and hybrids in many tropical and subtropical areas. populations of xanthomonas axonopodis pv. citri in leaf and twig lesions are the most important inoculum source for secondary infections. in areas with a marked winter season (e.g., argentina and japan), low temperatures induce a decrease of 10(2) to 10(4) in population sizes in lesions, thus creating a discontinuity in the x. axonopodis pv. citri life cycle. the ... | 2002 | 18942946 |
| geo-referenced spatiotemporal analysis of the urban citrus canker epidemic in florida. | abstract five areas in urban miami were identified to study the spread of xanthomonas axonopodis pv. citri to determine if the practice of removing exposed citrus trees within 38.1 m of trees affected by citrus canker was adequate to curtail further bacterial spread. to accomplish this, 18,769 trees in dooryards were surveyed, geo-referenced by differential global positioning systems (gps), and assayed for disease severity, age of infection, citrus cultivar, location of infection in tree, and ca ... | 2002 | 18942949 |
| disease development and symptom expression of xanthomonas axonopodis pv. citri in various citrus plant tissues. | abstract experimental inoculations of xanthomonas axonopodis pv. citri in different tissues of tahiti lime and pineapple sweet orange were conducted monthly under natural conditions on réunion island. the interactions between a set of environmental and epidemic variables associated with disease expression and 184 different factor combinations were investigated to determine the parameters needed to explain asiatic citrus canker (acc) disease expression. area under the disease progress curve (audp ... | 2003 | 18943164 |
| pathogenic interactions between xanthomonas axonopodis pv. citri and cultivars of pummelo (citrus grandis). | abstract the aggressiveness of strains of xanthomonas axonopodis pv. citri on seven citrus species, including citrus sinensis (navel orange), c. paradisi (grapefruit), c. unshiu (satsuma mandarin), c. junos (yuzu), c. aurantifolia ('mexican' lime), c. tachibana (tachibana), and c. grandis (pummelo: 'otachibana', 'banpeiyu', and 'anseikan'), were assessed by comparing lesion expansion and growth in planta, using a prick inoculation method. the existence of two groups distinct in aggressiveness wa ... | 2000 | 18943380 |
| improved sampling methods for real-time polymerase chain reaction diagnosis of citrus canker from field samples. | abstract citrus bacterial canker disease has been introduced at least three times into florida in the last 15 years and, despite federal and state quarantine and eradication efforts, continues to spread in florida. accurate, fast, and reliable detection of the causal agent is of great importance. however, citrus bacterial canker is caused by at least two groups of phylogenetically distinct xanthomonas citri strains, and there is host range variation within both groups. we developed a fast, sensi ... | 2004 | 18943820 |
| small non-coding rnas in streptomyces coelicolor. | in bacteria, small rnas (srnas) make important regulatory contributions to an ever increasing number of cellular processes. to expand the repertoire of known srnas, we sought to identify novel srnas in the differentiating, multicellular bacterium streptomyces coelicolor. we describe a combined bioinformatic and experimental approach that enabled the identification and characterization of nine novel srnas in s. coelicolor, including a cis-encoded antisense srna. we examined srna expression throug ... | 2008 | 19008244 |
| xanthomonas axonopodis pv. citri uses a plant natriuretic peptide-like protein to modify host homeostasis. | plant natriuretic peptides (pnps) are a class of extracellular, systemically mobile molecules that elicit a number of plant responses important in homeostasis and growth. the bacterial citrus pathogen, xanthomonas axonopodis pv. citri, also contains a gene encoding a pnp-like protein, xacpnp, that shares significant sequence similarity and identical domain organization with plant pnps but has no homologues in other bacteria. we have expressed and purified xacpnp and demonstrated that the bacteri ... | 2008 | 19015524 |
| transcriptional analysis of the sweet orange interaction with the citrus canker pathogens xanthomonas axonopodis pv. citri and xanthomonas axonopodis pv. aurantifolii. | xanthomonas axonopodis pv. citri (xac) and xanthomonas axonopodis pv. aurantifolii pathotype c (xaa) are responsible for citrus canker disease; however, while xac causes canker on all citrus varieties, xaa is restricted to mexican lime, and in sweet oranges it triggers a defence response. to gain insights into the differential pathogenicity exhibited by xac and xaa and to survey the early molecular events leading to canker development, a detailed transcriptional analysis of sweet orange plants i ... | 2008 | 19018992 |
| acquisition and evolution of plant pathogenesis-associated gene clusters and candidate determinants of tissue-specificity in xanthomonas. | xanthomonas is a large genus of plant-associated and plant-pathogenic bacteria. collectively, members cause diseases on over 392 plant species. individually, they exhibit marked host- and tissue-specificity. the determinants of this specificity are unknown. | 2008 | 19043590 |
| from local surveys to global surveillance: three high-throughput genotyping methods for epidemiological monitoring of xanthomonas citri pv. citri pathotypes. | asiatic citrus canker is a major disease worldwide, and its causal agent, xanthomonas citri pv. citri, is listed as a quarantine organism in many countries. analysis of the molecular epidemiology of this bacterium is hindered by a lack of molecular typing techniques suitable for surveillance and outbreak investigation. we report a comparative evaluation of three typing techniques, amplified fragment length polymorphism (aflp) analysis, insertion sequence ligation-mediated pcr (is-lm-pcr) typing, ... | 2009 | 19088309 |
| populations of xanthomonas citri pv. mangiferaeindicae from asymptomatic mango leaves are primarily endophytic. | epiphytic survival of several xanthomonas pathovars has been reported, but most studies failed to determine whether such populations were resident epiphytes, resulting from latent infections, or casual epiphytes. this study aimed at understanding the nature of xanthomonas citri pv. mangiferaeindicae populations associated with asymptomatic leaves. when spray-inoculated on mango leaves cv. maison rouge, the pathogen multiplied markedly in association with juvenile leaves, but was most often detec ... | 2009 | 19139953 |
| new genes of xanthomonas citri subsp. citri involved in pathogenesis and adaptation revealed by a transposon-based mutant library. | citrus canker is a disease caused by the phytopathogens xanthomonas citri subsp. citri, xanthomonas fuscans subsp. aurantifolli and xanthomonas alfalfae subsp. citrumelonis. the first of the three species, which causes citrus bacterial canker type a, is the most widely spread and severe, attacking all citrus species. in brazil, this species is the most important, being found in practically all areas where citrus canker has been detected. like most phytobacterioses, there is no efficient way to c ... | 2009 | 19149882 |
| crystallization, data collection and data processing of maltose-binding protein (male) from the phytopathogen xanthomonas axonopodis pv. citri. | maltose-binding protein is the periplasmic component of the abc transporter responsible for the uptake of maltose/maltodextrins. the xanthomonas axonopodis pv. citri maltose-binding protein male has been crystallized at 293 k using the hanging-drop vapour-diffusion method. the crystal belonged to the primitive hexagonal space group p6(1)22, with unit-cell parameters a = 123.59, b = 123.59, c = 304.20 a, and contained two molecules in the asymetric unit. it diffracted to 2.24 a resolution. | 2009 | 19193996 |
| a filamentous hemagglutinin-like protein of xanthomonas axonopodis pv. citri, the phytopathogen responsible for citrus canker, is involved in bacterial virulence. | xanthomonas axonopodis pv. citri, the phytopathogen responsible for citrus canker has a number of protein secretion systems and among them, at least one type v protein secretion system belonging to the two-partner secretion pathway. this system is mainly associated to the translocation of large proteins such as adhesins to the outer membrane of several pathogens. xanthomonas axonopodis pv. citri possess a filamentous hemagglutinin-like protein in close vicinity to its putative transporter protei ... | 2009 | 19194503 |
| phylogenetic structure of xanthomonas determined by comparison of gyrb sequences. | previously, we have produced a phylogeny of species type strains from the plant-pathogenic genus xanthomonas based on gyrb sequences. to evaluate this locus further for species and infraspecies identification, we sequenced an additional 203 strains comprising all the pathovar reference strains (which have defined plant hosts), 67 poorly characterized pathovars, currently classified as xanthomonas campestris, and 59 unidentified xanthomonads. the well-characterized pathovars grouped either in cla ... | 2009 | 19196764 |
| polyphasic characterization of xanthomonads pathogenic to members of the anacardiaceae and their relatedness to species of xanthomonas. | we have used amplified fragment length polymorphism (aflp), multilocus sequence analysis (mlsa) and dna-dna hybridization for genotypic classification of xanthomonas pathovars associated with the plant family anacardiaceae. aflp and mlsa results showed congruent phylogenetic relationships of the pathovar mangiferaeindicae (responsible for mango bacterial canker) with strains of xanthomonas axonopodis subgroup 9.5. this subgroup includes x. axonopodis pv. citri (synonym xanthomonas citri). simila ... | 2009 | 19196770 |
| identification of xanthomonas citri ssp. citri host specificity genes in a heterologous expression host. | we provide the first conclusive evidence that xanthomonas axonopodis pv. citri asiatic strain (xac-a) and, in particular, xac-a(w), a unique citrus canker a strain isolated from key lime in wellington, florida, induces a hypersensitive reaction (hr) in grapefruit leaves. using the heterologous tomato pathogen x. perforans, as a recipient of the xac-a(w) genomic library, we identified a 1599-bp open reading frame responsible for hr in grapefruit, but not key lime, and designated it avrgf1. xac-a( ... | 2009 | 19236573 |
| expression, crystallization and preliminary crystallographic analysis of pilz(xac1133) from xanthomonas axonopodis pv. citri. | proteins containing pilz domains are widespread in gram-negative bacteria and have recently been shown to be involved in the control of biofilm formation, adherence, aggregation, virulence-factor production and motility. furthermore, some pilz domains have recently been shown to bind the second messenger bis(3'-->5')cyclic digmp. here, the cloning, expression, purification and crystallization of pilz(xac1133), a protein consisting of a single pilz domain from xanthomonas axonopodis pv. citri, is ... | 2009 | 19255490 |
| characterization of citrus sinensis type 1 mitochondrial alternative oxidase and expression analysis in biotic stress. | the higher plant mitochondrial electron transport chain contains an alternative pathway that ends with the aox (alternative oxidase). the aox proteins are encoded by a small gene family composed of two discrete gene subfamilies. aox1 is present in both monocot and eudicot plants, whereas aox2 is only present in eudicot plants. we isolated a genomic clone from citrus sinensis containing the aox1a gene. the orange aox1a consists of four exons interrupted by three introns and its promoter harbours ... | 2009 | 19257856 |
| comparative ultrastructure of nonwounded mexican lime and yuzu leaves infected with the citrus canker bacterium xanthomonas citri pv. citri. | ultrastructural aspects of citrus canker development were investigated in nonwounded leaves of citrus species by transmission electron microscopy (tem). a susceptible species mexican lime and a resistant species yuzu were spray-inoculated with a virulent strain of xanthomonas citri pv. citri. initial symptoms occurred on mexican lime approximately 9 days after inoculation, whereas they appeared on yuzu mostly 11 days after inoculation. in mexican lime leaves, the bacterial invasion was usually a ... | 2009 | 19291686 |
| xanthomonas axonopodis pv. citri enters the vbnc state after copper treatment and retains its virulence. | the most severe form of citrus canker disease is caused by xanthomonas axonopodis pv. citri (xac) and affects all types of important citrus crops, reducing fruit yield and quality. copper-based products are routinely used as a standard control measure for citrus canker. in this work we demonstrate that copper treatment induces the viable but nonculturable (vbnc) state in xac but does not prevent the development of symptoms in susceptible plants. short-term exposures to different concentrations o ... | 2009 | 19624747 |
| cyclic di-gmp allosterically inhibits the crp-like protein (clp) of xanthomonas axonopodis pv. citri. | the protein clp from xanthomonas axonopodis pv. citri regulates pathogenesis and is a member of the crp (cyclic amp receptor protein) superfamily. we show that unlike the dna-binding activity of other members of this family, the dna-binding activity of clp is allosterically inhibited by its effector and that cyclic di-gmp serves as that effector at physiological concentrations. | 2009 | 19633082 |
| pilz protein structure and interactions with pilb and the fimx eal domain: implications for control of type iv pilus biogenesis. | the pilz protein was originally identified as necessary for type iv pilus (t4p) biogenesis. since then, a large and diverse family of bacterial pilz homology domains have been identified, some of which have been implicated in signaling pathways that control important processes, including motility, virulence and biofilm formation. furthermore, many pilz homology domains, though not pilz itself, have been shown to bind the important bacterial second messenger bis(3'-->5')cyclic digmp (c-digmp). th ... | 2009 | 19646999 |
| amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of xanthomonas citri pv. citri and xanthomonas campestris pv. bilvae. | three pathogenic variants (i.e. pathotypes) have been described within xanthomonas citri pv. citri, the causal agent of asiatic citrus canker. pathotype a strains naturally infect a wide range of citrus species and members of some related genera. in contrast, pathotypes a* and a(w) have narrow host ranges within the genus citrus and have been isolated from mexican lime (citrus aurantifolia l.) and from mexican lime and alemow (citrus macrophylla l.), respectively. we used amplified fragment leng ... | 2010 | 19654364 |
| modulating host homeostasis as a strategy in the plant-pathogen arms race. | in plant-pathogen interactions, pathogens aim to overcome host defense responses while plants employ a battery of responses to limit pathogen growth and thus disease. in this "arms race" between hosts and pathogens, horizontal gene transfer is a potent source of 'pathogenic innovation' for viruses and bacteria. however, bacteria rarely acquire 'eukaryotic-like' genes from their hosts, and where they appear to, evidence for a role of the acquired genes remains outstanding. we have recently report ... | 2009 | 19704897 |
| single-residue changes in the c-terminal disulfide-bonded loop of the pseudomonas aeruginosa type iv pilin influence pilus assembly and twitching motility. | pila, the major pilin subunit of pseudomonas aeruginosa type iv pili (t4p), is a principal structural component. pila has a conserved c-terminal disulfide-bonded loop (dsl) that has been implicated as the pilus adhesinotope. structural studies have suggested that dsl is involved in intersubunit interactions within the pilus fiber. pila mutants with single-residue substitutions, insertions, or deletions in the dsl were tested for pilin stability, pilus assembly, and t4p function. mutation of eith ... | 2009 | 19717595 |
| volatile antimicrobials from muscodor crispans, a novel endophytic fungus. | muscodor crispans is a recently described novel endophytic fungus of ananas ananassoides (wild pineapple) growing in the bolivian amazon basin. the fungus produces a mixture of volatile organic compounds (vocs); some of the major components of this mixture, as determined by gc/ms, are propanoic acid, 2-methyl-, methyl ester; propanoic acid, 2-methyl-; 1-butanol, 3-methyl-;1-butanol, 3-methyl-, acetate; propanoic acid, 2-methyl-, 2-methylbutyl ester; and ethanol. the fungus does not, however, pro ... | 2010 | 19797357 |
| optimal strategies for the eradication of asiatic citrus canker in heterogeneous host landscapes. | abstract the eradication of nonnative plant pathogens is a key challenge in plant disease epidemiology. asiatic citrus canker is an economically significant disease of citrus caused by the bacterial plant pathogen xanthomonas citri subsp. citri. the pathogen is a major exotic disease problem in many citrus producing areas of the world including the united states, brazil, and australia. various eradication attempts have been made on the disease but have been associated with significant social and ... | 2009 | 19900003 |
| a new member of the ribbon-helix-helix transcription factor superfamily from the plant pathogen xanthomonas axonopodis pv.citri. | xacb0070 is an uncharacterized protein coded by the two large plasmids isolated from xanthomonas axonopodis pv. citri, the agent of citrus canker and responsible for important economical losses in citrus world production. xacb0070 presents sequence homology only with other hypothetical proteins belonging to plant pathogens, none of which have their structure determined. the nmr-derived solution structure reveals this protein is a homodimer in which each monomer presents two domains with differen ... | 2010 | 20060909 |
| requirement of the galu gene for polysaccharide production by and pathogenicity and growth in planta of xanthomonas citri subsp. citri. | xanthomonas citri subsp. citri is the causal agent of citrus canker, which has a significant impact on citrus production. in this study, we characterized the galu gene of x. citri subsp. citri. two galu mutants (f6 and d12) were identified in an x. citri subsp. citri ez-tn5 <r6kgammaori/kan-2> tnp transposon library. rescue cloning, sequence analysis, and southern blot analysis indicated that both of these mutants had a single copy of the ez-tn5 transposon inserted in galu in the chromosome. fur ... | 2010 | 20118360 |
| optical fiber laser induced fluorescence spectroscopy as a citrus canker diagnostic. | citrus canker is a serious disease caused by xanthomonas citri subsp. citri bacteria, which infects citrus plants (citrus spp.) leading to large economic losses in citrus production worldwide. in this work, laser induced fluorescence spectroscopy (lif) was investigated as a diagnostic technique for citrus canker disease in citrus trees at an orchard using a portable optical fiber based spectrometer. for comparison we have applied lif to leaves contaminated with citrus canker, citrus scab, citrus ... | 2010 | 20119016 |
| a eukaryotic-acquired gene by a biotrophic phytopathogen allows prolonged survival on the host by counteracting the shut-down of plant photosynthesis. | xanthomonas citri pv. citri, the bacteria responsible for citrus canker posses a biological active plant natriuretic peptide (pnp)-like protein, not present in any other bacteria. pnps are a class of extracellular, systemically mobile peptides that elicit a number of plant responses important in homeostasis and growth. previously, we showed that a xanthomonas citri pv. citri mutant lacking the pnp-like protein xacpnp produced more necrotic lesions in citrus leaves than wild type infections and s ... | 2010 | 20126632 |
| hpaxm from xanthomonas citri subsp. malvacearum is a novel harpin with two heptads for hypersensitive response. | a novel harpin-like protein, hpaxm, was described from cotton leaf blight bacteria, xanthomonas. citri subsp. malvacearum. the hpaxm was found to be localized between hrp2 and hrcc. a phylogenetic analysis of the complete amino acid sequence or solely the 13 highly conserved residues h2n-sekqldqlltqli-cooh in the n-terminal alpha-helix indicates that hpaxm is evolutionarily closer to hpagxag and hpaxac than to hpa1xoo and hpa1xoc. a synthesized peptide containing two heptads, 39-ldqlltq-limallq- ... | 2010 | 20134233 |
| broad spectrum microarray for fingerprint-based bacterial species identification. | microarrays are powerful tools for dna-based molecular diagnostics and identification of pathogens. most target a limited range of organisms and are based on only one or a very few genes for specific identification. such microarrays are limited to organisms for which specific probes are available, and often have difficulty discriminating closely related taxa. we have developed an alternative broad-spectrum microarray that employs hybridisation fingerprints generated by high-density anonymous mar ... | 2010 | 20163710 |
| phylogenomics reveals a diverse rickettsiales type iv secretion system. | with an obligate intracellular lifestyle, alphaproteobacteria of the order rickettsiales have inextricably coevolved with their various eukaryotic hosts, resulting in small, reductive genomes and strict dependency on host resources. unsurprisingly, large portions of rickettsiales genomes encode proteins involved in transport and secretion. one particular transporter that has garnered recent attention from researchers is the type iv secretion system (t4ss). homologous to the well-studied archetyp ... | 2010 | 20176788 |
| a plant natriuretic peptide-like molecule of the pathogen xanthomonas axonopodis pv. citri causes rapid changes in the proteome of its citrus host. | plant natriuretic peptides (pnps) belong to a novel class of peptidic signaling molecules that share some structural similarity to the n-terminal domain of expansins and affect physiological processes such as water and ion homeostasis at nano-molar concentrations. the citrus pathogen xanthomonas axonopodis pv. citri possesses a pnp-like peptide (xacpnp) uniquely present in this bacteria. previously we observed that the expression of xacpnp is induced upon infection and that lesions produced in l ... | 2010 | 20302677 |
| novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of xanthomonas fuscans subsp. aurantifolii. | citrus canker is a disease that has severe economic impact on the citrus industry worldwide. there are three types of canker, called a, b, and c. the three types have different phenotypes and affect different citrus species. the causative agent for type a is xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. xanthomonas fuscans subsp. aurantifolii strain b causes canker b and xanthomonas fuscans subsp. aurantifolii strain c causes canker c. | 2010 | 20388224 |
| bacteria causing important diseases of citrus utilise distinct modes of pathogenesis to attack a common host. | in this review, we summarise the current knowledge on three pathogens that exhibit distinct tissue specificity and modes of pathogenesis in citrus plants. xanthomonas axonopodis pv. citri causes canker disease and invades the host leaf mesophyll tissue through natural openings and can also survive as an epiphyte. xylella fastidiosa and candidatus liberibacter are vectored by insects and proliferate in the vascular system of the host, either in the phloem (candidatus liberibacter) or xylem (x. fa ... | 2010 | 20449739 |
| development and validation of a xanthomonas axonopodis pv. citri dna microarray platform (xacarray) generated from the shotgun libraries previously used in the sequencing of this bacterial genome. | abstract: | 2010 | 20507617 |
| the monofunctional catalase kate of xanthomonas axonopodis pv. citri is required for full virulence in citrus plants. | xanthomonas axonopodis pv. citri (xac) is an obligate aerobic phytopathogen constantly exposed to hydrogen peroxide produced by normal aerobic respiration and by the plant defense response during plant-pathogen interactions. four putative catalase genes have been identified in silico in the xac genome, designated as kate, catb, srpa (monofunctional catalases) and katg (bifunctional catalase). | 2010 | 20520822 |
| genomes of three tomato pathogens within the ralstonia solanacearum species complex reveal significant evolutionary divergence. | the ralstonia solanacearum species complex includes thousands of strains pathogenic to an unusually wide range of plant species. these globally dispersed and heterogeneous strains cause bacterial wilt diseases, which have major socio-economic impacts. pathogenicity is an ancestral trait in r. solanacearum and strains with high genetic variation can be subdivided into four phylotypes, correlating to isolates from asia (phylotype i), the americas (phylotype iia and iib), africa (phylotype iii) and ... | 2010 | 20550686 |
| xanthomonas axonopodis pv. citri: factors affecting successful eradication of citrus canker. | summary taxonomic status: bacteria, proteobacteria, gamma subdivision, xanthomodales, xanthomonas group, axonopodis dna homology group, x. axonopodis pv. citri (hasse) vauterin et al. microbiological properties: gram negative, slender, rod-shaped, aerobic, motile by a single polar flagellum, produces slow growing, non-mucoid colonies in culture, ecologically obligate plant parasite. host range: causal agent of asiatic citrus canker on most citrus spp. and close relatives of citrus in the family ... | 2004 | 20565577 |
| rapid and sensitive detection of citrus bacterial canker by loop-mediated isothermal amplification combined with simple visual evaluation methods. | citrus bacterial canker (cbc) is a major, highly contagious disease of citrus plants present in many countries in asia, africa and america, but not in the mediterranean area. there are three types of citrus bacterial canker, named a, b, and c that have different genotypes and posses variation in host range within citrus species. the causative agent for type a cbc is xanthomonas citri subsp. citri, while xanthomonas fuscans subsp. aurantifolii, strain b causes type b cbc and xanthomonas fuscans s ... | 2010 | 20565886 |
| xanthomonas citri: breaking the surface. | summary taxonomy: bacteria; proteobacteria, gammaproteobacteria; xanthomonadales; xanthomonadaceae, xanthomonas. microbiological properties: gram-negative, obligately aerobic, straight rods, motile by a single polar flagellum, yellow pigment. related species: x. campestris, x. axonopodis, x. oryzae, x. albilineans. host range: affects rutaceous plants, primarily citrus spp., fortunella spp., and poncirus spp., world-wide. quarantined pathogen in many countries. economically important hosts are c ... | 2003 | 20569374 |
| subcellular localization of proteins labeled with gfp in xanthomonas citri ssp. citri: targeting the division septum. | xanthomonas citri ssp. citri (xac) is the causal agent of citrus canker, an economically important disease that affects citrus worldwide. to initiate the characterization of essential biological processes of xac, we constructed integrative plasmids for the ectopic expression of green fluorescent protein (gfp)-labeled proteins within this bacterium. here, we show that the disruption of the alpha-amylase gene (amy), the site of plasmid integration into the bacterial chromosome, does not alter its ... | 2010 | 20629754 |
| the xanthomonas citri effector protein ptha interacts with citrus proteins involved in nuclear transport, protein folding and ubiquitination associated with dna repair. | xanthomonas axonopodis pv. citri utilizes the type iii effector protein ptha to modulate host transcription to promote citrus canker. ptha proteins belong to the avrbs3/ptha family and carry a domain comprising tandem repeats of 34 amino acids that mediates protein-protein and protein-dna interactions. we show here that variants of pthas from a single bacterial strain localize to the nucleus of plant cells and form homo- and heterodimers through the association of their repeat regions. we hypoth ... | 2010 | 20696004 |
| transmission of plant-pathogenic bacteria by nonhost seeds without induction of an associated defense reaction at emergence. | an understanding of the mechanisms involved in the different steps of bacterial disease epidemiology is essential to develop new control strategies. seeds are the passive carriers of a diversified microbial cohort likely to affect seedling physiology. among seed-borne plant-pathogenic bacteria, seed carriage in compatible situations is well evidenced. the aims of our work are to determine the efficiency of pathogen transmission to seeds of a nonhost plant and to evaluate bacterial and plant beha ... | 2010 | 20729326 |
| shedding light on the role of photosynthesis in pathogen colonization and host defense. | the role of photosynthesis in plant defense is a fundamental question awaiting further molecular and physiological elucidation. to this end we investigated host responses to infection with the bacterial pathogen xanthomonas axonopodis pv. citri, the pathogen responsible for citrus canker. this pathogen encodes a plant-like natriuretic peptide (xacpnp) that is expressed specifically during the infection process and prevents deterioration of the physiological condition of the infected tissue. prot ... | 2010 | 20798833 |