Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| altered immune responsiveness associated with encephalitozoon cuniculi infection in rabbits. | the variation in immune response of two unrelated colonies of laboratory rabbits to high doses of heat-killed brucella abortus strain 19 was investigated. one was a mixed-breed, multicolored colony in which a high prevalence of encephalitozoonosis had been recorded, whereas the other rabbits were derived from a colony of dutch-marked specific-pathogen-free rabits. although considerable variation in the immune response between individual rabbits was noticed at all bleeds, rabbits infected with en ... | 1977 | 139377 |
| modulation of immune response by killed brucella abortus organisms: comparison of the effects of smooth and rough strains on t-dependent responses. | inactivated brucella abortus organisms of the smooth (s) or rough (r) strain were tested comparatively on two t-dependent immune responses: mixed-lymphocyte reaction and delayed-type hypersensitivity. the intravenous injection of s organisms depressed the two tests, whereas r organisms increased mixed-lymphocyte reaction and did not alter delayed-type hypersensitivity significantly. this observation may be helpful in understanding the differences in adjuvant properties of s and r brucellae. | 1978 | 152297 |
| [isolation of brucella abortus biotypes 1 and 4 in nicaragua]. | 1979 | 158368 | |
| erythritol catabolism by brucella abortus. | cell extracts of brucella abortus (british 19) catabolized erythritol through a series of phosphorylated intermediates to dihydroxyacetonephosphate and co-2. cell extracts required adenosine 5'-triphosphate (atp), nicotinamide adenine dinucleotide (nad), mg2+, inorganic orthophosphate, and reduced glutathione for activity. the first reaction in the pathway was the phosphorylation of mesoerythritol with an atp-dependent kinase which formed d-erythritol 1-phosphate (d-erythro-tetritol 1-phosphate) ... | 1975 | 163226 |
| inhibition of growth by erythritol catabolism in brucella abortus. | the growth of brucella abortus (us-19) in a complex tryptose-yeast extract medium containing d-glucose is inhibited by 10 mm erythritol. the enzymes of the erythritol pathway, except for d-erythrulose 1-phosphate dehydrogenase (d-glycero-2-tetrulose 1-phosphate:nicotinamide adenine dinucleotide (nad+) 4-oxidoreductase) were detected in the soluble and membrane fractions of cell extracts. glucose catabolism by cell extracts was inhibited by erythritol, whereas, phosphorylated intermediates of the ... | 1975 | 170249 |
| some effects of silical treatment on marek's disease. | treatment of newly hatched chicks with silica by the intraperitoneal route delayed the onset of mortalities due to the jm strain of marek's disease (md' virus inoculated at 6 days of age. during the 88-day observation period fewer silica-treated chicks died of md, but this difference was not usually statistically significant. silica treatment had no effect on the susceptibility of 4-week-old birds. silica treatment reduced the antibody response to md but, in general, not significantly. the antib ... | 1976 | 179947 |
| antiviral activity of an extract of brucella abortus: induction of interferon and immunopotentiation of host resistance. | 1976 | 181760 | |
| [carboyydrates localization on ultrathin sections of "brucella" and "escherichia" cells in smooth (s) and rough (r) phase (author's transl)]. | carbohydrates localization on ultrathin sections of brucella abortus, melitensis and excherichia coli cells has been studied by the periodic acid thiocarbohydrazide-silver proteinate (patag) and phosphotungstic acid (pta) methods. carbohydrates are mainly localized on the cell envelope of brucella and escherichia but there are several differences between these two bacteria. the differences are discussed and a brucella polysaccharide envelope model is proposed. the patag method gives the same sil ... | 1976 | 187096 |
| the role of bursa-dependent responses in immunity to infectious laryngotracheitis. | the effect of combined day-old surgical bursectomy and cyclophosphamide therapy on the response of young chicks to brucella abortus antigen and cloacal vaccination against infectious laryngotracheitis virus (iltv) was examined. the combined treatment effectively prevented the development of serum antibody to both b abortus and iltv, yet when these chickens were challenged with virulent iltv, they were found to be immune. it is suggested that immunity to iltv may depend on the development of a ce ... | 1977 | 195320 |
| depression of vaccinal immunity to marek's disease by infection with reticuloendotheliosis virus. | the effect of infection with low-virulence, tissue culture-propagated strains of reticuloendotheliosis virus (rev) on protective vaccinal immunity against marek's disease (md) lymphomas was investigated. vaccinated chickens inoculated at hatching with greater than 10(4) focus-forming units of rev and challenged with md virus were poorly protected against md lesion development as indicated by protective indices of 53 to 79% for strain cs (p less than 0.05) and 42 to 49% for strain t (p less than ... | 1979 | 227800 |
| measurement of immunosuppression in chickens caused by infectious bursal disease vaccines using brucella abortus strain 19. | the serological response of chicks to brucella abortus strain 19 was monitored over a period of seven weeks to assess the degree of immunosuppression caused by vaccination at one day of age with two infectious bursal disease vaccines. one of the vacy. this vaccine caused severe immunosuppression judged by the minimal serological response following b abortus inoculation. the test also detected a significant delay caused by the other vaccine in the development of the serological response but the m ... | 1979 | 230558 |
| characterization of the electron transport system in brucella abortus. | the electron transport system in brucella abortus has been characterized. spectral studies of membrane preparations have indicated the presence of cytochromes a + a3 (maxima at 612 nm), cytochrome b (maxima at 560, 530, and 428 nm), cytochrome c (maxima at 552 and 522 nm), cytochrome o (maxima of carbon monoxide complex at 418 nm), and flavoproteins (minimum at 582 and 450 nm). cytochromes a + a3 appeared only after cells had reached late log phase, possibly due to lowered oxygen tension in the ... | 1975 | 235507 |
| [influence of brucella abortus on the course of acute myeloid leukemia in mice]. | 1977 | 268723 | |
| activation of reticuloendothelial system macrophages and enhancement of host resistance to a transplantable osteogenic sarcoma in mice by an extract of brucella abortus. | an aqueous-ether extract of brucella abortus, bru-pel, enhanced resistance of mice to a transplantable osteogenic sarcoma (ogs). the results presented in this report suggest that bru-pel is an effective immunomodulator and that one mechanism through which it enhances host resistance is activation of phagocytic cells of the reticuloendothelial system. peritoneal macrophages from mice inoculated with bru-pel 14 days previously were cytotoxic for ogs cells in vitro, limited the multiplication of va ... | 1978 | 282007 |
| absence of b stem cells in spleens of chickens fed the androgen analog mibolerone. | bursa-derived stem cells in the spleens of chickens treated with the androgen analog mibolerone or with cyclophosphamide and in the spleens of untreated controls were examined by a transplantation technique. spleen cells from syngeneic donors 2-20 weeks old were transferred to syngeneic cyclophosphamide-treated recipients, and the immunocompetence of the recipients was tested 5 weeks after the transfer by antigenic stimuli with sheep erythrocytes and brucella abortus. spleens of mibolerone-treat ... | 1978 | 306248 |
| lymhocyte stimulatory effect of an ether-extracted preparation of brucella abortus. | the effect of a brucella abortus preparation (bru pel) on the t- and b-lymphocyte populations is described. bru pel treatment of tumor-bearing mice resulted in a slight reduction in tumor size, marked splenomegaly, and statistically significant reductions in the percentages of splenic t and b lymphocytes relative to untreated tumor controls. bru pel was also found to cause increases in the incorporation of 3h-thymidine of phytohemagglutinin-sensitive splenic t lymphocytes and lipopolysaccharide- ... | 1978 | 310345 |
| the role of the thymus for maturation of transferred bursa cells into immunocompetent b cells in chickens treated with cyclophosphamide. | chickens injected with cyclophosphamide and x-ray irradiated in the newly hatched period were immunized with a mixture of sheep red blood cells, brucella abortus and salmonella pullorum at 4, 5 and 6 weeks of age, and were examined for serum antibody titres, serum immunoglobulin concentration and bursal and splenic structures at 7 weeks of age. the neonatal treatments suppressed completely or almost completely antibody responses, immunoglobulin production and formation of bursal follicles and sp ... | 1978 | 310807 |
| ontogeny of susceptibility of mouse splenic b cells to tolerance induction in vitro by tnp-d-gl. | the susceptibility of mouse spleen cells to hapten-specific tolerance induction of a primary in vitro thymus-independent antibody response was examined. both the induction of tolerance by 2,4,6-trinitrophenyl-d-glutamic acid-d-lysine (tnp96d-gl) and of antibody formation (elicited by tnp-brucella abortus) in neonatal spleen cell cultures were unaffected by anti-thy-1.2 plus complement treatment. spleen cells from neonatal mice were only slightly more sensitive to tnp96d-gl tolerance induction th ... | 1979 | 312201 |
| intraembryonic origin of lymphoid stem cells in the chicken: studies with sex chromosome and igg allotype markers in histocompatible yolk sac-embryo chimaeras. | two-day-old chick embryonic bodies were transplanted onto the area vasculosa of age-matched histocompatible blastoderms, resulting in the development of yolk sac-embryo chimaeras. eighteen of these succeeded in hatching and became adults. differences in the sex chromosomes and in igg allotype between the embryo and the yolk sac were used to study the contribution of these two components to the lymphoid cell development. at 5-7 weeks of age the chimaeras proved to be completely normal in the igm ... | 1979 | 316918 |
| the ontogeny of thymic independent antibody responses in vitro in normal mice and mice with an x-linked b cell defect. | the primary in vitro antibody response of neonatal spleen cells to three thymic independent antigens has been examined. the time of onset of responsiveness to tnp-brucella abortus and tnp-lipopolysaccharide was significantly earlier than the onset of responsiveness to tnp-ficoll. this ontologic sequence was not affected by t cell depletion or antigen presentation on adult macrophages. in neonatal mice bearing the x-linked cba/n defect, the response to tnp-brucella abortus and tnp-lipopolysacchar ... | 1977 | 334975 |
| comparison of the histamine hypersensitivity and the limulus amoebocyte lysate tests for endotoxin activity. | the histamine hypersensitivity test and the limulus amoebocyte lysate test were compared for their effectiveness to quantitate endotoxin activity. the two tests compared favorably in all the trials, except with a sample of endotoxin from brucella abortus that gave a positive limulus amoebocyte lysate test at a concentration of 0.001 microgram, while failing to sensitize mice to histamine at a dose of 16 microgram per mouse. the limulus amoebocyte lysate test was more sensitive than the histamine ... | 1978 | 365749 |
| protective effects of supplemental vitamin e against infection. | vitamin e supplementation (dl-alpha-tocopheryl acetate except where noted) in excess of requirement significantly increased humoral immune response or disease resistance. mice immunized with sheep red blood cells or tetanus toxoid and fed the supplemental vitamin demonstrated increased plaque-forming cells (pfc) and hemagglutinin (ha) titers. a vitamin e deficiency resulted in decreased pfc and little igg which was partially corrected by n,n-diphenyl-p-phenylenediamine but not as effectively as ... | 1979 | 376353 |
| use of an enzyme immunoassay test for characterizing the a and m antigens of brucella. | an enzyme immunoassay test was developed for detecting the a and m antigens of brucellae. one hundred fifteen isolates, including 96 strains of brucella abortus, 5 strains of b. suis, 1 strain of b. melitensis and 1 strain of b. neotomae, were accurately serotyped with the enzyme immunoassay test. no reactions were observed with 11 isolates of b. canis and 1 isolate of b. ovis. this rapid serological test provides for considerable savings in time and materials as compared with the standard tube ... | 1979 | 379038 |
| an immunological enhancement phenomenon in experimental brucella infection of the chicks. | the passive transfer of chicken anti-brucella immune globulins promote the survival of brucella abortus in the spleen of chickens infected with this bacteria. this enhancing effect was reduced significantly when the immune globulins were absorbed with heat-killed brucella cells to remove all the anti-brucella agglutinins and immune adherence antibodies. therefore, it is most probable that the enhancing effect is related to the presence of brucella antibodies. | 1977 | 403146 |
| on the growth of brucella species in presence of erythritol in defined and undefined media and amniotic fluid of human, cow and sheep. | different concentrations of erythritol (25-1000 microng/ml) in defined medium were shown to enhance the growth of 75.00 to 91.66 per cent of brucella abortus and 69.23 to 100.00 per cent of brucella melitensis strains studied. compared to these figures only 13.04 to 60.86% (in tryptose broth), and 41.40 to 62.50% (in glycerol dextrose broth), of brucella abortus strains, 25.00 to 83.30 per cent (in tryptose broth) and 83.30 to 100.00 per cent (in glycerol dextrose broth) of brucella melitensis s ... | 1977 | 403714 |
| [antibacterial and antimycotic activity of the amniotic fluid against selected germs (author's transl)]. | 60 samples of amniotic fluid from 60 patients were obtained between 14 and 42 weeks gestation by dates and tested for their antibacterial activity against staphlococcus aureus strains and brucella abortus and their antimycotic activity against candida albicans. the antibacterial and antimycotic activity of the amniotic fluid was confirmed. this activity increases steadily from the 15th week and reaches its maximum around term between 36 and 42 weeks. the amniotic samples obtained at 20 weeks ges ... | 1977 | 404210 |
| mitogenic activity of cell wall components from smooth and rough strains of brucella abortus. | on the basis of [3h]thymidine incorporation by normal mouse spleen cell cultures, cell wall preparations from a smooth (45/0) strain and a rough (45/20) strain of brucella abortus were strongly mitogenic. on the other hand, none of several subcomponents extracted from the cell wall preparations, including aqueous and phenolic lipopolysaccharides, was active. these results contrast with the marked mitogenic activity of lipopolysaccharides isolated from other gram-negative bacteria such as salmone ... | 1977 | 404248 |
| antitumor activity of a brucella abortus preparation. | mice injected intraperitoneally with sarcoma-180 cells develop ascites and eventually die. intraperitoneal injection of a nonviable, aqueous... ether-extracted brucella abortus preparation (bru-pel) as early as 7 days before or as late as 7 days after injection of tumor cells significantly inhibited development of ascites and protected against death. bru-pel was not effective if injected after ascites was grossly apparent. bru-pel was significantly more active than a corynebacterium parvum prepa ... | 1977 | 404249 |
| the bovine immune response to brucella abortus i. a water soluble antigen precipitated by sera of some naturally infected cattle. | selected sera from cattle naturally infected with brucella abortus precipitate water soluble antigens extracted by sonication from b. abortus. one of these antigens resembles antigen e (baughn and freeman) as it is excluded from sephadex g-200 gels, migrates anodally when electrophoresed at ph 8.6, resists heating at 100 degrees c for ten minutes and appears to be susceptible to papain digestion. precipitins specific for this antigen remained in sera from which all detectable brucella agglutinat ... | 1977 | 405088 |
| responsiveness of rabbit spleen and appendix cells to bacterial mitogens. | rabbit spleen and appendix cells were used to test the mitogenic activity of a commercial lipopolysaccharide (lps) preparation from salmonella typhimurium (difco), a preparation extracted from it, and cell wall preparations from smooth (45/0) and rough (45/20) strains of brucella abortus. on the basis of [3h]thymidine incorporation ratios (e/c), that is, the incorporation rate among cells treated with the mitogen relative to that of untreated cells, the extracted lps and both brucella cell wall ... | 1977 | 405322 |
| immunostimulation by brucella abortus. role of surface antigenicity of the bacteria. | 1977 | 406141 | |
| the isolation of brucella abortus biotype i from african buffalo in the kruger national park. | the isolation of brucella abortus from free living wild africal buffalo (syncerus caffer) in the kruger national park, south africa, is described. the four isolates testes proved to be biotype i and it is speculated that the origin of brucellosis in wild animals is from domestic stock. | 1977 | 406393 |
| the tranfer of brucella abortus antibodies from dam to calf. | the higher sensitivity of the abgt (coombs test) makes it the most useful test for comparison of total specific immunoglobulins for brucella abortus in different secretions. using it in the present study it was found that in 85 cows the mean selective concentration of immunoglobulins in colostrum was six to eight fold, whether the cows were infected or not. in the non-infected group calves acquired mean levels of immunoglobulins of about one third those in colostrum whereas in the infected group ... | 1977 | 406728 |
| smooth phage-resistant brucella abortus from bovine tissue. | conventional typing, oxidative metabolic, and virulence tests were conducted on a phage-resistant brucella abortus strain isolated from the supramammary lymph node of a cow. | 1977 | 407247 |
| contribution to the standardization of brucella abortus antigens and antisera for agglutination tests by photo-electric measurement. | variations in the dose-response curve were graphically and arithmetically represented for the serum agglutination test in brucellosis, instances being given of the use in field conditions of a method of standardizing antigens and testing reference sera compared with standard preparations. | 1977 | 407669 |
| identification of anti-a + m and anti-r antibodies in cattle vaccinated with rough strain brucella abortus. | 1977 | 407704 | |
| influence on kinetics of response in igm and igg in persistent and conjunct immunization with two antigens: "brucella abortus" and sheep erythrocytes. | 1977 | 407781 | |
| antibacterial activity of amniotic fluid against staphylococcus aureus, candida albicans and brucella abortus. | sixty amniotic fluid samples from sixty patients between 14--42 weeks gestation were studied for antibacterial activity against staphylococcus aureus, brucella abortus and antifungal activity against candida albicans. both antibacterial and antifungal activity of the amniotic fluid were observed. when antimicrobial activity was correlated with gestational age, it was found to increase with the period of gestation. maximum antimicrobial activity of the amniotic fluid was found to be present in th ... | 1977 | 407884 |
| formation of antibody in the newborn mouse: study of t-cell-independent antibody response. | the ontogeny of immune responsiveness, as assayed by antibody formation in vitro, of mouse spleen lymphocytes to thymus-independent antigens is reviewed. responsiveness to trinitrophenyl (tnp)-lipopolysaccharide and tnp-brucella abortus appear soon after birth and one to two weeks before tnp-ficoll or capsular polysaccharide of streptococcus pneumoniae (sss-iii) elicits significant antibody formation. this hierarchy of responsiveness to antigens is also apparent in the cba/n mutant mouse strain, ... | 1977 | 408430 |
| a solid phase assay with radioactively-labelled antibody for the detection of brucella abortus. | an assay for the detection of brucella abortus is described. igg from rabbit antisera against live brucellae or brucella extracts was chemically linked to cellulose to form a solid phase reagent capable of binding brucella antigens present in buffer solutions or serum. after washing away any unbound material the presence of bound antigen was revealed by incubation with radioactively-labelled anti-brucella antibodies. the assay was capable of detecting less than 100 pg brucella antigen in a 20 mi ... | 1977 | 408491 |
| [chemical and serological studies of brucella abortus 99 bacterial wall fractions]. | mahadevan and tatum's method was used to isolate four fractions from the bacterial walls of brucella abortus 99. determined were the amino acid composition and the purity (by means of the complement fixation test) of these fractions. infrared spectroscopy was carried out as well. it was found that: 1. the particular fractions differ by their amino acid composition. 2. the fractions are practically pure. 3. the functional groups established confirm previous data of the author on the chemical comp ... | 1977 | 408968 |
| factors determining the effects of chronic protein-deficiency on antibody responses to sheep red blood cells and brucella abortus vaccine in mice. | chronic protein-deficiency in weanling mice caused variable suppression of the humoral plaque-forming cell (pfc) responses to sheep erythrocytes. this was most prominent at high antigen doses and did not increase when mice were maintained on the diets for longer periods. antibody responses produced by deficient mice were often short-lived and involved high levels of igm. total pfc counts were depressed slightly more than were circulating antibodies. antibody responses to brucella abortus were sl ... | 1977 | 409385 |
| salvage of a valuable bull affected with unilateral brucella abortus orchitis. | 1977 | 409386 | |
| antibacterial and anthelminthic properties of visnaginone and khellinone derivatives. | the compounds tested, derivatives of visnaginone and khellinone, showed antibacterial activity on gram negative and gram positive micro-organisms. some of the compounds possess an antibacterial activity on some pathogenic bacteria, i.e. brucella abortus, for which there is no remedy as yet. all the chalcones tested were inactive except ib, which showed antiparasitic broad spectrum. | 1977 | 409417 |
| rifampin in the treatment of experimental brucellosis in mice and guinea pigs. | rifampin, a broad-spectrum antibiotic able to penetrate intracellularly, was used for treatment of infections with brucella melitensis in mice and brucella abortus in guinea pigs. treatments were administered for seven, 14, or 21 days; mice were given 25 mg of rifampin/kg per day, and guinea pigs 100 mg/kg per day. efficacy of the drug was determined by comparison of rifampin-treated animals with saline-treated controls and with tetracycline-treated mice (200 mg/kg per day) according to the foll ... | 1977 | 409786 |
| application of the indirect enzyme-labeled antibody microtest to the detection and surveillance of animal diseases. | the rapid, indirect enzyme-labeled antibody (ela) microplate test has been developed as a diagnostic and surveillance tool to aid in the control of animal disease. the test has been applied to viral (hog cholera), parasitic (trichinosis), and bacterial (brucellosis) diseases of animals. a correlation of greater than 95% was observed between the hog cholera ela test and the serum neutralization test for hog cholera in greater than 2,000 field samples obtained during the 1976 epizootic in new jers ... | 1977 | 409789 |
| the interaction of "brucella abortus" 544 and neutrophil polymorphonuclear leucocytes. | 1977 | 410375 | |
| enhancement activity of anti-brucella sera in experimental brucella abortus infection in mice. | the passive transfer of rabbit anti-brucella sera promotes the multiplication of brucella abortus in the spleen of mice infected intravenously with a low dose of this bacteria. the enhancement activity of the anti-brucella sera is related to their modes of preparation in rabbits. the agglutinin titers of all these antisera were relatively high, while there was some discrepancies in their content in haemagglutinating, immune adherence and complement fixing antibodies. | 1977 | 412235 |
| further observations on the inhibition of tumor growth by corynebacterium parvum with cyclophosphamide. v. comparison of the effects of tilorone hydrochloride, levamisole, methanol-soluble fraction of mycobacterium butyricum, bcg, and a nonviable aqueous ether extract of brucella abortus preparation in treatment of mice with tumors. | 1978 | 413929 | |
| [antibacterial activity of the amniotic fluid against staphylococcus aureus, candida albicans, and brucella abortus (proceedings)]. | 1977 | 414693 | |
| abortion in a mare associated with brucella abortus infection and twins. | 1977 | 415408 | |
| [allergic reactions in guinea pigs infected with yersinia enterocolitica type 9 relative to homologous (yersinia) and heterologous (brucella) allergens]. | obtained were allergens from brucella abortus 99 and yersinia enterocolitica type 9 through hydrolysis with hydrochloric acid after m. m. ivanov and with acetic acid after kirzhaev. they were tested on guinea pigs that were infected with yersinia enterocolitica type 9 through different ways. to m. m. ivanov's allergens all infected animals responded pronouncedly in the case of the homologous allergen, and half of them only and to a lower degree in the case of the heterologous brucella allergen. ... | 1977 | 415410 |
| kinetics of in vitro bovine lymphocyte immunostimulation with a brucella abortus antigen. | a brucella abortus-soluble antigen was investigated, using in vitro assay of lymphocyte immunostimulation, to determine which concentration of this antigen and which period of incubation of the lymphocyte cultures would induce maximum specific lymphocyte immunostimulation as an additional method for further study of b abortus infection in cattle. soluble antigen was prepared from autoclaved cells of b abortus strain 1119-3. peripheral blood lymphocytes were obtained from cattle infected with b a ... | 1978 | 415646 |
| a comparison of three selective media for the isolation of brucella abortus from contaminated material. | 1977 | 415792 | |
| comparison of two challenge strains used to test the potency of brucella abortus vaccines. | 1977 | 415793 | |
| brucellosis in elk i. serologic and bacteriologic survey in wyoming. | incidence of brucellosis in elk (cervus canadensis) on two winter feedgrounds in wyoming was examined over a 5-year period by testing serum samples using the standard plate agglutination (spt) buffered brucella antigen (bba), rivanol (riv) and complement fixation (cft) tests. thirty-one percent of 1,165 elk were positive by defined criteria. considering each test individually, only 29% (106) of 370 positive sera would have been classified as reactors by the spt, 83% (307) by the bba test and 86% ... | 1978 | 416232 |
| chemical and biological adjuvants capable of potentiating tumor cell vaccine. | with an l1210 tumor vaccine model, three biological and two chemical agents were tested for their ability to act as adjuvants. adjuvant was administered with irradiated l1210 cells to immunize mice against this poorly immunogenic tumor. two chemicals, pyran copolymer and glucan, and one biological, brucella abortus strain 456 ether extract, were shown to be strong stimulators of antitumor immunity. vaccination with irradiated tumor cells or adjuvant alone did not produce host resistance. optimal ... | 1978 | 416906 |
| recovery of brucella abortus in culture from a 5 ml sample of bovine blood. | calvings in a herd infected by brucella abortus were monitored by cultural examination of milk and mucus and by serological and cultural examination of 5 ml samples of the blood. out of 129 samples from which culture of b abortus was attempted, only one yielded a positive result. | 1978 | 417452 |
| specific lymphocyte stimulation in cattle naturally infected with strains of brucella abortus and cattle vaccinated with brucella abortus strain 19. | cell-mediated immune responses in cattle naturally infected with strains of brucella abortus and in cattle vaccinated with b abortus strain 19 during calfhood were studied by an in vitro lymphocyte-stimulation procedure. lymphocytes were prepared from peripheral bovine blood by the ficoll-diatrizoate technique, suspended in rpmi-1640 medium (1.5 x 10(6) lymphocytes/ml), cultured with b abortus-soluble antigen or phytohemagglutinin, and incubated for 6 days. sixteen hours prior to termination of ... | 1978 | 417648 |
| the use of case history studies to differentiate potentially infected from potentially noninfected herds with reactors to brucella abortus antigens. | data on farm characteristics and the results of the first herd test for brucellosis were collected for 74 reactor and 74 negative herds in wellington county, ontario. each reactor herd was classified as either probably infected or probably not infected using the occurrence of abortions prior to the first herd test or during the testing period, the total number of cattle removed and/or the spread of reactors within the herd as criteria of infection. statistical techniques were used to select vari ... | 1978 | 417777 |
| brucella abortus in the bitch: subclinical infection associated with urinary excretion. | brucella abortus infection in the bitch, associated with an aborted bovine fetus, is described. clinical signs were absent, but the organism was isolated from many sites in the body, including the urine, after death. the response of the dog to b. abortus infection and its possible role as a vector of the disease is discussed. | 1979 | 429789 |
| brucella abortus arthritis. | 1979 | 454511 | |
| the effect of early treatment with methyl-ccnu on the lymphoid organs and the humoral immune response in chickens. | presented are data upon the effect of a single or muliple administration of methyl-ccnu (a nitrosourea derivative an alkylating agent, and anticancer drug) to newly-hatched chicks. lethal and sublethal doses of methyl-ccnu caused temporary damage to the lymphoid organs, of which the bursa was apparently the most sensitive. the decreased weight and the morphological changes observed in the bursa of treated chicks were accompanied by a parallel decrease in serum gamma-globulins level. the humoral ... | 1979 | 471882 |
| prebursal stem cells in the intraembryonic mesenchyme of the chick embryo at 7 days of incubation. | cyclophosphamide-treated 18-day-old chick embryos were transplanted with cells from 7-day intraembryonic mesenchyme; the recipients and donors were identical at the major histocompatibility locus. at the age of 35 days, the cell recipients were studied to determine the reconstitution capacity of the transplanted cells. the transplantation resulted in a complete restoration of igm and igg class antibody production against human gammaglobulin and brucella abortus, and of microscopic morphology of ... | 1979 | 489971 |
| formation of germinal centres during neonatal tolerance in the chicken. | chickens were made tolerant to bsa at the time of hatching. during the tolerance, antigenic stimulation with bsa resulted in poor germinal centre formation as compared to normal immunized control birds. the tolerance persisted for at least 6 weeks. its breakdown had hardly started at the age of 12 weeks, both igm and igg antibody responses against bsa remaining negligible. stimulation with the unrelated antigens srbc and brucella abortus resulted in good antibody responses, but the number of ger ... | 1979 | 495104 |
| isolation and properties of a phage lytic for non-smooth brucella organisms. | a phage for non-smooth cells of brucella abortus was isolated from a mixture of three brucella-phages incubated with rough brucella cells in the presence of n-methyl-n'-nitro-n-nitrosoguanidine. it did not lyse smooth cells of br. abortus strains nor those of other brucella species, but during the course of its replication in rough organisms a small proportion of phage mutants were produced which were similar in properties to smooth-specific phages. the rough-specific phage, r, itself resembled ... | 1979 | 536378 |
| bursal fabricii as a peripheral lymphoid organ. transport of various materials from the anal lips to the bursal lymphoid follicles with reference to its immunological importance. | in tracer studies, various particulate and molecular materials applied on the anal lips of chickens were transported to the bursal lumen. from here some of the tracers were further transported into the bursal lymphoid follicles and colloidal carbon even to the bursal stroma. in immunization experiments, brucella abortus organisms and srbc were applied on the anal lips of 1-day-old, 4-week-old and 10-week-old chicks on 5 consecutive days. although brucella bacteria in tracer studies were not foun ... | 1978 | 564868 |
| polyadenylic acid-polyuridylic acid (poly a : u) and experimental murine brucellosis. i. effect of single and double-stranded polynucleotides on brucella abortus in vivo and in vitro. | the double stranded polynucleotide, poly a : u, when administered intraperitoneally at the same time as intravenous infection with brucella abortus, suppressed the growth of that organism in the spleen and liver of mice. single stranded poly a or poly u were not effective. on the other hand both the single and double stranded forms enhanced the growth of br. abortus in broth culture. poly a : u did not enhance the blood clearance of intravenously administered br. abortus. indeed its suppressive ... | 1978 | 680803 |
| the persistence of brucella abortus infection in calves: a retrospective study of heavily infected herds. | a retrospective inquiry into 170 herds which were slaughtered in the first three years of the brucella eradication scheme found that in the 115 herds where all animals were slaughtered there were no subsequent breakdowns, other than in herds which were restocked with imported animals. in the 46 herds in which only the adult stock were slaughtered, 11 herds suffered breakdowns. in 10 of these 11 herds the sources of reinfection were possibly reprieved heifers. seven out of the 11 initial reactors ... | 1978 | 695262 |
| evaluation of new and currently used diagnostic procedures for bovine brucellosis. | the indirect haemolysis test (ihlt) and the rough antigen complement-fixation test (rcft) were compared with several conventional tests using serum samples from 9 cows known to be infected with brucella abortus. in 7 cows all the tests except the rcft (which was developed to detect antibodies resulting from 45/20 vaccination) became positive and remained so until the cows were autopsied 6 months after infection. in the other 2 cows the rose bengal test was occasionally negative and the titre of ... | 1978 | 708329 |
| effect of halothane anaesthesia on secondary antibody response and mitogen-induced lymphocyte transformation in the chicken. | the effect of a single halothane anaesthesia on the secondary antibody response and the lymphocyte response in vitro to phytohaemagglutinin (pha) and concanavalin a(con a) was studied. the antigens used were bovine serum albumin (bsa) and killed brucella abortus organisms (brucella). igg and igm antibodies against these antigens were quantified by solid-phase radioimmunoassay. lymphocyte transformation was studied by a whole blood micromethod. the chickens were anaesthetised for two hours with 2 ... | 1978 | 716924 |
| overview of the activity of a brucella abortus preparation, bru-pel. | the properties of a nonviable, aqueous ether-extracted brucela abortus preparation, bru-pel, are described. in addition to inducing a "virus-type" interferon response and protecting mice against challenge with otherwise lethal doses of semliki forest virus, bru-pel is demonstrated to have potent antitumor properties in mice. these antitumor effects appear to be mediated by an increase in nonspecific resistance similar to that seen with other experimental antitumor agents. | 1978 | 728911 |
| comparative effects of corynebacterium parvum, brucella abortus extract, bacillus calmette-guérin, glucan, levamisole, and tilorone with or without cyclophosphamide on tumor growth, macrophage production, and macrophage cytotoxicity in a murine mammary tumor model. | in this laboratory, it has been repeatedly demonstrated (using a murine mammary tumor model) that the combination of cyclophosphamide (cy) and corynebacterium parvum (cp) is more effective than either agent alone in the control of tumor growth. this paper presents information obtained in our model comparing findings on the effects of cp with a brucella abortus extract (bru-pel; bp) and glucan (gl) on tumor growth. in addition, the influence of those agents as well as bacillus calmette-guérin, ti ... | 1978 | 728912 |
| [growth of some pathogenic microorganisms on chemically defined media which were proposed for sensitivity testing (author's transl)]. | the growth of 16 bacterial cultures, which belong to the frequently encountered pathogens, in three different chemically defined fluid media was investigated through turbidity measurements in comparison with mueller-hinton-broth. e. coli, salm. dublin, salm. typhi-murium, enterobacter cloacae, proteus mirabilis, pseud. aeruginosa and bac. cereus grew in two of the three tested defined media with approximately the same intensity as in the mueller-hinton broth. staph. aureus grew well only in one ... | 1978 | 730218 |
| bovine immunoglobulins and brucellosis. 3. activity of igg, igg2 and igm versus different commercial batches of rose bengal antigen. | the serological diagnosis of bovine brucellosis by the rose bengal test has been disputed on account of the amount of active immunoglobulins and the results obtained in field trials. in order to try and explain these discrepancies, we have compared the activity of different commercial preparations of the rose bengal antigen for purified igg1, igg2 or igm dies. two antigens which give the same titer for the ig1 differ by a factor 8 in the titration of the igm. some antigens titer the igg2, others ... | 1978 | 749644 |
| [immuno-stimulating effect of levamisole in the immunization of guinea pigs vaccinated against brucellosis]. | the immuno-stimulating levamisole action was checked by the authors in guinea-pigs vaccinated against brucellosis and exposed to the challenge test afterwards against 2.308 brucella abortus pathogenic strain. twelve lots of eight guinea-pigs were used disposed in the following distribution: lots i, iii, iv were vaccinated with b-19 strain vaccine; lots ii, iv and vi were vaccinated with the same vaccine and, after 48 hours, received the levamisole injection. lots vii viii received only the levam ... | 1978 | 754679 |
| [structural and immunochemical studies on the immunoglobulin of carp (cyprinus carpio l.). iii. valence and affinity of antidinitrophenyl-antibodies]. | carps were immunized with dnp conjugated to human serum albumin, bovine gamma globulin and brucella abortus. the antibodies were isolated by immunoadsorption. the intrinsic average association constant (ko) of the antibodies was determined by equilibrium dialysis and ammonium sulphate precipitation employing the monovalent hapten tritiated epsilon-dnp-l-lysine. the tetrameric carp igm possesses four measurable combining sites per molecule. the affinity (ko) of the isolated antibodies varied from ... | 1975 | 788423 |
| immune mechanisms affecting bovine leucocytes during suppression of tuberculin skin sensitivity in cattle. | complete desensitization to tuberculin skin-testing (anergy) was produced in cattle by repeated intravenous injections of living bcg organisms into animals sensitized by a prior subcutaneous dose of bcg. two levels of desensitization were produced; complete desensitization following 10 i.v. doses and partial desensitization following 5 i.v. doses of 100 mg bcg. intradermal tuberculin testing at the end of the experiment stimulated the appearance of reactive blood lymphocytes in sensitized cattle ... | 1976 | 797673 |
| the effects of ascorbic acid depletion on the immune response of the guinea-pig to brucella abortus strain 19 vaccine. | 1975 | 805147 | |
| brucella abortus biotype 1 as a cause of abortion in a bitch. | 1975 | 806159 | |
| brucellosis and veterinary surgeons. | forty-six veterinary surgeons were given a full clinical examination, serological examinations with estimates of immunoglobulins, and supplementary haematological and radiological investigations. thirty-five complained of one or more symptoms, and eight had abnormal physical signs which might have been caused by infection with brucella abortus, but neither sereological tests results nor immunoglobulin estimates bore any special relation to the clinical features. the soundest way of assessing ill ... | 1975 | 806320 |
| brucellosis in childhood. | brucellosis has always been an unusual disease in children and, concomitant with the control of the disease in domestic animals, reports have become sparse. the pediatrician, therefore, may not be aware of the protean clinical manifestations of childhood brucellosis. in 1973, nine cases occurred during a three-month period in el paso, texas. all cases were marked by spiking fevers and lethargy of four days to four weeks in duration. tender hepatomegaly or splenomegaly was striking in seven patie ... | 1975 | 806883 |
| stimulation of igg antibody formation by sublethal irradiation during persistently repeated immunization with brucella abortas. | whole-body irradiation with 600 rads 8 or 10 days after the beginning of persistently repeated brucella abortus immunization in the rabbit produces little change in the response. by contrast, irradiation with the same dose 2, 4 or 6 days after the beginning of repeated immunization stimulates the igg response. this effect is explained by a repopulation of antigen-sensitive cells from precursor cells to a higher level than that previous to irradiation. in order to explain igg stimulation, three m ... | 1975 | 807518 |
| fluid and solid media for isolation of brucella abortus. | the use of a fluid-enrichment technique increased by 10-16% the recovery rate of brucella abortus from milks of herds positive to a milk ring test. three solid media for direct cultural techniques were also tested on the same milks. combinations of these techniques produced a recovery rate of ca. 88% from mrt-positive-herd milks using a mixture of the centrifuged deposit and cream layer as the inoculum as against a recovery rate of ca. 75% using the mrt cream layer as inoculum. b. abortus biotyp ... | 1975 | 807615 |
| activated macrophages in congenitally athymic "nude mice" and in lethally irradiate mice. | resistance to the facultative intracellular bacteria, brucella abortus and listeria monocytogenes, is principally the result of acquisition of enhanced antibacterial activity by host macrophages, probably in response to lymphokines released by t lymphocytes. however, the present paper describes a surprisingly high resistance on the part of both congenitally athymic "nude" mice and of lethally irradiated mice compared with normal controls. this enhanced bactericidal activity was evident 24 hr aft ... | 1975 | 807647 |
| brucellosis: a study of five calves from reactor dams. | five calves from brucella abortus reactor cows bought after they had been on infected farms for two to six weeks, were reared in isolation and examined serologically for approximately six months prior to cultural and biological examination post mertem. one calf which had been serologically negative to the rose-bengal, serum agglutination and complement fixation tests for the last eight weeks of its life yielded a culture of br abortus biotype 1. this serotype had also been isolated from its dam ... | 1975 | 808019 |
| the bursal origin of an immunocompetent cell for antibody formation in the chicken. | surgically bursectomized and irradiated chickens were given bursal, splenic, bone marrow or thymic cells taken from syngeneic donors, together with killed brucella abortus and salmonella pullorum. blood samples were taken from those chickens 7 days later, and the serum agglutinin titres were determined. the cells of any lymphoid organ taken from 28-day-old chickens were more effective in restoring antibody response than those from 18-day-old ones. the restorative capacities of the bursa and sple ... | 1975 | 808466 |
| [brucella isolated in france: identification and typing. i. -- brucella abortus (author's transl)]. | two hundred and eight strains of brucella isolated from cattle and 5 isolated from man in various areas of france, during a two-year period from 1972 to 1974, were examined by all methods recommended by the subcommittee on taxonomy of the genus brucella and were found to have the same characteristics that identify and define the species brucella abortus. of the 213 strains, 105 (49,29 p. 100) were biotype i, 103 strains (48,36 p. 100) biotype 3, three strains (1,41 p. 100) biotype 4 and 2 strain ... | 1975 | 808998 |
| [nitrofurantoin-test for the differentiation of bordetella bronchiseptica (author's transl)]. | bordetella bronchiseptica is primarily resistant against nitrofurantoin (mic greater than 200 mug/ml), and this feature can be used for the differentiation of the organism from other gram-negative coccobacteria. nitrofurantoin paper disks (300 mug) failed to affect the growth of 150 strains of b. bronchiseptica isolated from different animal hosts, but they produced marked inhibition zones in the cultures of the followingspecies: pasteurella multocida, pasteurella pneumotropica, pasteurella haem ... | 1975 | 809944 |
| differentiation of cross-reacting antibodies against brucella abortus and yersinia enterocolitica by electroimmuno assay. | 1975 | 810011 | |
| [development of spleenic infection in the mouse following intravenous inoculation of various species of brucella in smooth (s) or rough (r) phases]. | the bacterial population in the spleen of mice inoculated intravenously by brucella abortus 544 (s), brucella suis 1330 (s), brucella melitensis 115 (r) or brucella canis rm 666(r) is studied kinetically. like the s strains of brucella, the r strains are able to multiply in the mouse spleen. the consequences for the tests of vaccines against natural infections produced by s or r strains of brucella are discussed. | 1975 | 810263 |
| the inability of surgical bursectomy in the newly hatched period to affect antibody formation in the pigeon (columba livia). | surgical bursectomy was performed in newly hatched pigeons within 24 hours of hatching. the effect of bursectomy on the immune responsiveness was evaluated by studying antibody formation to sheep red blood cells (srbc) and brucella abortus organisms and by the presence of serum immunoglobulins after immunoelectrophoresis. surgically bursectomized pigeons responded as well to srbc and brucella as did the non-bursectomized controls. there was no statistically significant difference in the geometri ... | 1975 | 810416 |
| a mechanism for prozone formation in the complement fixation test for bovine brucellosis. | complement fixation (cf) by bovine igg1 or igm antibodies to brucella abortus was inhibited by specific non-complement-fixing antibodies of the igg2 subclass. this inhibition may account for the appearance of prozones in cf titrations of some antiserums, and for the occurrence of serums which are positive to the rose bengal test, but negative to the cf test. | 1975 | 811200 |
| stimulation of protective immunity to salmonella kauffmann-white group n serotypes by brucella abortus and yersinia enterocolitica antigens. | 1975 | 811321 | |
| [adjuvant activity of the peptidoglycan isolated from the bacterial wall of brucella abortus 99]. | cell walls were obtained from brucella abortus 99. a peptidoglucan was isolated from them. test animals were immunized (albino mice) with pure human gammaglobulin, and in a series of experiments the peptidoglucan was added to the antigen, while in another one it was injected 24 hours later. the titer of the separated serum was determined in terms of the amount of the complement-fixing antibodies present, using the microreaction of complement fixation. it was established that with the addition of ... | 1975 | 812253 |
| immunoglobulin class specificity of non-agglutinating antibody produced in cattle following brucella abortus 45/20 vaccination. | the immunoglobulin class specificity of non-agglutinating antibody produced following vaccination with br. abortus 45/20 vaccine was determined using the antiglobulin test. in previously unvaccinated cattle it was found that most of the non-agglutinating antibody was associated with the igg1 immunoglobulin class. in cattle sensitised to brucella antigens by prior vaccination with brucella abortus strain 19 the majority of antibody was also associated with the igg1 immunoglobulin class, but a sig ... | 1975 | 812466 |
| studies on a smooth phage resistant variant of brucella abortus ii. mechanism of phage resistance. | the properties of a smooth phage resistant variant of brucella abortus were studied in an attempt to determine the mechanism of phage resistance. this strain was fully capable of adsorbing phage but penetration, and hence replication, did not occur. no evidence of lysogeny or a phage carrier state could be obtained and gross chemical differences between the resistant strain and its phage susceptible parent were not detected. the phage resistant variant showed increased resistance to lysis from w ... | 1975 | 812543 |
| leukocyte migration inhibition by a specific antigen in human brucellosis. | leukocyte migration inhibition /lmi/ tests were carried out in 19 patients with brucella abortus bovis infection and in 18 healthy control subjects using killed brucella abortus bovis as antigen. in the brucellosis group a statistically significant lmi was observed, as compared to the members of the control group. using the same antigen, a fairly good correlation was found between the local skin reaction and the results of the lmi test in the subacute or chronic stage of the disease. however, th ... | 1975 | 812829 |
| the immunogenic activity of ribosomal fractions derived from brucella abortus. | the immunizing activity of ribosome preparations derived from brucella abortus strain 19 cells was examined in guinea-pigs and mice. after subcutaneous injections of br. abortus ribosomes in freund's incomplete adjuvant, both mice and guinea-pigs developed immunity to challenge by virulent br. abortus 544 organisms which was at least as effective as the protection conferred by live strain 19 vaccine. both mice and guinea-pigs also developed agglutinating and complement-fixing antibodies and dela ... | 1976 | 812900 |
| humoral immune responses characteristic of testosterone-propionate-treated chickens. | white leghorn chickens treated with testosterone-propionate on the 3rd day of embryonation were immunized with a mixture of sheep red blood cells, brucella abortus and salmonella pullorum at various ages, and the resulting agglutinins were titrated. the production of igm antibody against sheep red blood cells was not affected significantly by testosterone-propionate. on the contrary, immune responses against the bacterial antigens were strongly suppressed by the same treatment. production of igg ... | 1976 | 815175 |