Publications

campylobacter rectus in human periodontitis.	campylobacter rectus (formerly wolinella recta) in periodontitis lesions was studied relative to age and sex distribution, relationship to disease-active periodontitis, response to periodontal debridement and in vitro antimicrobial susceptibility. subgingival c. rectus was collected with paper points, transported in vmga iii and plated onto nonselective enriched brucella blood agar and hammond's selective medium for c. rectus, both incubated anaerobically. c. rectus was recovered from 80% of 165 ...	1993	8247610
role of suspected periodontopathogens in microbiological monitoring of periodontitis.	periodontal disease is the clinical result of a complex interaction between the host and plaque bacteria. although a specificity to some degree is found for actinobacillus actinomycetemcomitans in localized juvenile periodontitis (ljp), it has been difficult to obtain evidence for a specific etiological role of the bacteria associated with periodontal disease in adults. what we see is the net result of host-parasite interactions which in an unpredictable moment accumulate and exceed the threshol ...	1993	8260004
dna probe detection of periodontopathogens in advanced periodontitis.	species-specific dna probes were used to determine the presence of actinobacillus actinomycetemcomitans (a.a.), porphyromonas (bacteroides) gingivalis, prevotella intermedia, treponema denticola, eikenella corrodens, fusobacterium nucleatum, and wolinella recta in subgingival plaque from deep pockets/sites of patients with advanced periodontitis. the subjects were 20 patients with severe adult periodontitis, 13 men and 7 women (mean age 45.6 +/- 6.7 yr). for each subject, 9-10 subgingival sites ...	1993	8290878
isolation and partial characterization of the campylobacter rectus cytotoxin.	previously, we reported the antigranulocytic activity of campylobacter rectus media supernatants containing lipopolysaccharide (lps) and a 104 kda protein. here, we monitored the release of protein and lps through the growth cycle of c. rectus atcc 33238 and identified the 104 kda protein as the cytotoxin. lps in media supernatants was quantitated by a kdo assay; the 104 kda protein was detected on immunoblots with specific antibody (a104) and quantitated by amino acid analysis of membrane immob ...	1993	8321122
recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms.	the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ...	1993	8324113
oral and dental infections with anaerobic bacteria: clinical features, predominant pathogens, and treatment.	microbial populations colonizing the teeth are a major source of pathogens responsible for oral and dental infections, including periodontal diseases, gingivitis, pericoronitis, endodontitis, peri-implantitis, and postextraction infections. each entity has distinct clinical and microbial features. bacterial species associated with oral infections include actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, bacteroides forsythus, campylobacter rectus, eubacterium ...	1993	8324136
suppression of proliferation of a human b-cell leukaemic cell line derived from acute lymphoblastic leukaemia by soluble factor(s) from campylobacter rectus.	soluble sonic extracts of several strains were examined for their ability to alter proliferation of a cell line derived from acute lymphoblastic leukaemia (ball-1). extracts of all strains tested caused dose-dependent suppression of proliferation when assessed by dna (tritiated thymidine incorporation), rna (tritiated uridine incorporation) and protein (tritiated leucine incorporation) synthesis. there was no effect on the viability of ball-1 as measured by either trypan-blue exclusion or extrac ...	1993	8343067
helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis.	four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ...	1993	8379970
microbiological and clinical monitoring of non-localized juvenile periodontitis in young adults: a report of 11 cases.	it has been shown that patients with localized juvenile periodontitis (ljp) often harbor actinobacillus actinomycetemcomitans in the subgingival area. however, little is known of the oral microflora in non-ljp juvenile periodontitis patients with less extensive disease. the purpose of this study was to describe the microflora and clinical parameters of young adults with minor to moderate periodontitis during treatment for a period of 1 year. eleven patients 15 to 16 years of age were studied. al ...	1993	8381179
effect of therapy on periodontal infections.	periodontal disease progression requires the simultaneous presence of high numbers of pathogens, low numbers of compatible or beneficial species, a conductive local environment, and a susceptible host. effective therapy acts by altering one or more of these factors. data from an ongoing study were used to examine the biological basis of treatment success or failure. seventeen subjects showing disease progression were treated by widman flap surgery at deep sites, scaling at shallow sites, and 1 o ...	1993	8410615
microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis.	a series of 993 subgingival microbial samples sent to a diagnostic microbiology laboratory included 196 samples that could be identified as compatible with a clinical diagnosis of refractory or recurrent periodontitis. in descending order of prevalence the associated microbiota included bacteroides forsythus (84%), spirochetes (83%), motile rods (76%), fusobacterium species (68%), porphyromonas gingivalis (63%), campylobacter rectus (47%), capnocytophaga species (38%), prevotella intermedia (23% ...	1993	8463936
an analysis of the subgingival microflora in randomly selected subjects.	the aim of this study was to describe the presence of some microbial species in the subgingival microflora of a randomly selected subject sample. a further objective was to analyze the association between some microbiological characteristics and the periodontal conditions of the subjects. a total of 171 individuals aged 30-65 years were included. a pooled subgingival plaque sample was obtained from the mesial aspect of the 6 ramfjord teeth by the use of the paper-point method. the samples were p ...	1993	8510980
aspartame as a source of essential phenylalanine for the growth of oral anaerobes.	phenylalanine and aspartic acid requirements were determined for 13 species of oral bacteria using the chemically defined medium omiz-w1. none of actinobacillus actinomycetemcomitans, bacteroides forsythus, eikenella corrodens, selenomonas sputigena, treponema pectinovorum, t. socranskii, or wolinella recta required either of these amino acid constituents of aspartame (l-aspartyl-l-phenylalanine methylester). phenylalanine was essential for the growth of capnocytophaga gingivalis, eubacterium ti ...	1993	8514113
antigenic properties of campylobacter rectus (wolinella recta) major s-layer proteins.	the antigenic properties of the surface layer (s-layer) proteins of various campylobacter rectus strains including 24 clinical isolates and the type strain atcc 33238 were examined. s-layer proteins were extracted from whole cells by acid treatment according to the method of mccoy et al. (infect. immun. 11, 517-525, 1975). the acid extracts from 23 of the isolates and atcc 33238 contained two major proteins with molecular masses of 130 kda and 150 kda, both of which were identified as subunits o ...	1993	8514115
salivary levels of suspected periodontal pathogens in relation to periodontal status and treatment.	the primary ecological niche for suspected periodontal pathogens seems to be the subgingival area, even though periodontal pathogens are also frequently recovered from saliva. the interrelationship of different periodontal conditions and the salivary levels of suspected periodontal pathogens is not known. in the present study, salivary levels of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, and peptostreptococcus micros were determin ...	1995	8530742
oral microbiota in subjects with a weak or strong response in experimental gingivitis.	the purpose of the present study was to examine the composition of the oral microbiota in subjects who had previously demonstrated to develop either a weak or strong response to experimental gingivitis. for this study, subjects were selected from a pool of 25 individuals who had participated twice in an experimental gingivitis trial. out of these 25 panellists, 6 subjects were selected who had developed 2x a weak gingival inflammatory response and 7 subjects who had developed 2x a strong gingiva ...	1995	8583022
cell envelope and cell wall immunization of macaca fascicularis: effect on the progression of ligature-induced periodontitis.	the nonhuman primate, macaca fascicularis, was used to study the role of immunization with selected members of the periodontopathic microbiota in the longitudinal progression of ligature-induced periodontitis. animals were immunized with cell envelope antigens prepared from porphyromonas gingivalis and prevotella intermedia, and a mixture prepared from fusobacterium nucleatum, campylobacter rectus, and actinomyces viscosus. serum immunoglobulin g (igg), igm and iga isotype antibodies increased s ...	1995	8602339
bacteriological diagnosis of periodontal disease.	dental plaque bacteria cause virtually all the forms of inflammatory periodontal disease. periodontitis is caused by the specific periodontopathic bacteria, which induce destruction of connective tissue attachment and adjacent alveolar bone. examinations to identify the infections by porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia, campylobacter rectus, bacteroides forsythus, fusobacterium nucleatum, eikenella corrodens and treponema denticola have recently ...	1994	8620588
comparison of randomly cloned and whole genomic dna probes for the detection of porphyromonas gingivalis and bacteroides forsythus.	whole genomic and randomly-cloned dna probes for two fastidious periodontal pathogens, porphyromonas gingivalis and bacteroides forsythus were labeled with digoxigenin and detected by a colorimetric method. the specificity and sensitivity of the whole genomic and cloned probes were compared. the cloned probes were highly specific compared to the whole genomic probes. a significant degree of cross-reactivity with bacteroides species, capnocytophaga sp. and prevotella sp. was observed with the who ...	1996	8636873
periodontal status and detection frequency of bacteria at sites of periodontal health and gingivitis.	it is generally recognized that bacteria in dental plaque at sites of periodontal diseases are not commonly found at sites of periodontal health. one hypothesis to explain the etiology of periodontitis is that pathogenic bacteria from diseased sites infect healthy sites. it has been suggested that gingival inflammation may predispose sites to colonization by bacteria associated with periodontal diseases. the purpose of this cross-sectional study was to determine whether the detection frequency o ...	1996	8667130
effect of initial therapy on dynamics of immunogloblin g levels to some periodontopathic bacteria in serum and gingival crevicular fluid.	using enzyme-linked immunosorbent assay, the igg antibody levels to specific gram-negative periodontopathic bacteria in serum of 21 adult periodontitis patients and the gingival crevicular fluid (gcf) from 42 sites with destructive lesions and 21 healthy sites were evaluated before and after initial preparation. porphyromonas gingivalis, bacteroides forsythus, prevotella intermedia, prevotella nigrescens, campylobacter rectus, treponema denticola and actinobacillus actinomycetemcomitans in subgi ...	1994	8689734
comparison of the pro-inflammatory cytokine-stimulating activity of the surface-associated proteins of periodontopathic bacteria.	saline extraction of the periodontopathic bacterium, actinobacillus actinomycetemcomitans, releases surface-associated material (sam), a complex mixture of proteins and carbohydrates with potent biological actions on isolated bone and on various mammalian cell populations. in this study, the relative ability of the sam from 5 organisms, implicated in the pathology of periodontal disease, to stimulate human mesenchymal and myelomonocytic cells to synthesize the proinflammatory cytokines - interle ...	1996	8708939
production of inflammatory mediators and cytokines by human gingival fibroblasts following bacterial challenge.	bacteria can indirectly affect the course of periodontal diseases by activating host cells to produce and release inflammatory mediators and cytokines. these mediators and cytokines manifest potent proinflammatory and catabolic activity and may play key roles in local amplification of the immune response as well as in periodontal tissue breakdown. this study tested the effect of actinobacillus actinomycetemcomitans (aa) and campylobacter rectus (cr) challenge on pge2, il-1 beta, il-6 and il-8 pr ...	1996	8708945
in vitro senescence enhances il-6 production in human gingival fibroblasts induced by lipopolysaccharide from campylobacter rectus.	the production of interleukin-6 (il-6) in human gingival fibroblasts (gin cells) is increased by lipopolysaccharide (lps) from campylobacter rectus (c. rectus), which is associated with adult periodontitis; however, the age-related changes in the susceptibility of gin cells to c. rectus lps remain unclear. we examined the influence of in vitro senescence on c. rectus lps-stimulated il-6 production in gin cells. lps was prepared from c. rectus atcc 33238 using hot phenol-water. the gin cells were ...	1996	8735906
histological and microbiological aspects of ligature-induced periodontitis in beagle dogs.	this study was designed to investigate, using 6 beagle dogs, the levels of selected putative pathogens in healthy sites, in gingivitis sites, and in sites with histologically confirmed attachment loss. levels of attachment loss increased with increasing periods of ligation and reached a maximum of 0.15 mm at 57 days. both histological attachment level and histological pocket depth were found to vary significantly with health/disease status (p < 0.0001). higher numbers of total colony-forming uni ...	1996	8739161
utility of 5 major putative periodontal pathogens and selected clinical parameters to predict periodontal breakdown in patients on maintenance care.	the predictive utility of 5 major putative periodontopathic microbial species, "superinfecting" organisms, and several clinical periodontal parameters were assessed relative to periodontitis recurrence over a 12-month period in 78 treated adult patients participating in a 3-month maintenance care program. at baseline, pooled subgingival microbial samples were collected from each patient, and whole-mouth evaluations of probing depth, relative periodontal attachment level, furcation involvement, a ...	1996	8739166
treatment of periodontal disease based on microbiological diagnosis. relation between microbiological and clinical parameters during 5 years.	the purpose of this study was to assess the clinical effect of treatment aimed to suppress actinobacillus actinomycetemcomitans and porphyromonas gingivalis below detection level and prevotella intermedia below 5% of the cultivable periodontal pocket flora. sixteen patients and a total of 111 periodontal pockets with probing depth > or = 6 mm were included in the study. twelve patients and a total of 77 sites completed the 5-year study. the results demonstrated clinical improvement of probing de ...	1996	8794965
source of suspected periodontal pathogens re-emerging after periodontal treatment.	to clarify the source of re-emerging periodontal pathogens after treatment, we compared the ribotypes of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia/prevotella nigrescens group and campylobacter rectus before and after treatment in 7 periodontitis patients and in 6 of the spouses. the patients harbored a. actinomycetemcomitans, p. gingivalis, p. intermedia/p. nigrescens group or c. rectus in their subgingival or salivary samples before treatment. the res ...	1996	8811482
evaluation of the long-term efficacy and safety of locally-applied minocycline in adult periodontitis patients.	the objectives of the present study were to establish in a long-term investigation the safety as well as the clinical and microbiological efficacy of scaling and rootplaning combined with local application of 2% minocycline hydrochloride-gel versus placebo-gel in patients with moderate to severe chronic adult periodontitis. this was an 18 months, randomized, double-blind, parallel, comparative study, in which 20 healthy patients with moderate to severe chronic periodontitis participated. at base ...	1996	8877655
selling biotechnology in the dental medicine marketplace: the omnigene diagnostics dna probe tests for periodontal pathogens.	omnigene diagnostics, inc. has applied the principles of genetic engineering to develop species-specific dna probe tests for eight periodontal pathogens (porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetem-comitans, fusobacterium nucleatum, eikenella corrodens, campylobacter rectus, bacteroides forsythus, and treponema denticola). the test requires minimal effort on the part of the clinician: subgingival plaque samples are collected from the patient and sent through the ...	1996	8931245
associations between porphyromonas gingivalis and oral treponemes in subgingival plaque.	colonization and/or proliferation of treponema denticola may depend on the presence of porphyromonas gingivalis. the aims of this study were to confirm this synergistic relationship, to determine whether other oral bacteria were similarly associated with p. gingivalis and to relate coinfection to the periodontal status of plaque donors. subgingival plaque was collected from every tooth except third molars in 106 subjects who were grouped by their worst periodontal condition. in addition to p. gi ...	1996	8941768
the adherence of periodontopathogens to periodontal probes. a possible factor in intra-oral transmission?	periodontal probes have previously been shown to harbor several bacterial types or species after probing periodontally diseased pockets. this study aims to identify and quantify periodontopathogens that may adhere to a periodontal probe by culturing techniques. it also examines the probe's roughness on its capability to collect bacteria, comparing merrit-b probes (with deep indentations) with tps probes (with smooth surfaces). from the differential phase contrast microscopy findings it was seen ...	1996	8959565
degradation of lactoferrin by periodontitis-associated bacteria.	the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ...	1996	8961558
polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions.	a 16s rrna-based polymerase chain reaction (pcr) detection method was used to determine the prevalence of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, eikenella corrodens, porphyromonas gingivalis, prevotella intermedia. prevotella nigrescens and treponema denticola in subgingival specimens of 50 advanced periodontitis, 50 adult gingivitis and 50 pediatric gingivitis subjects. the optimal pcr conditions were determined for each study species. agarose gel ele ...	1996	9002880
plaque ph and microflora of dental plaque on sound and carious root surfaces.	given the hypothesis that root caries is the result of acid formation by acidogenic micro-organisms, the present study was performed to relate sucrose-induced ph response of dental plaque on root surfaces to the microbial composition of the overlying plaque. seventeen caries-active elderly chinese with poor oral hygiene and with both sound and carious root surfaces were examined. plaque ph was measured before and up to one hour after a controlled sucrose mouthrinse. plaque samples for microbiolo ...	1996	9003238
purification and characterization of campylobacter rectus surface layer proteins.	campylobacter rectus is a putative periodontopathogen which expresses a proteinaceous surface layer (s-layer) external to the outer membrane. s-layers are considered to play a protective role for the microorganism in hostile environments. the s-layer proteins from six different c. rectus strains (five human isolates and a nonhuman primate [nhp] isolate) were isolated, purified, and characterized. the s-layer proteins of these strains varied in molecular mass (ca. 150 to 166 kda) as determined by ...	1997	9009300
rapid diagnosis of periodontal infections: findings in aids patients.	a small number of bacterial pathogens in the human oral cavity cause the different forms of periodontal disease. of the approximately two hundred different oral bacterial species, about a dozen have been associated with these diseases including localized juvenile periodontitis, rapidly progressing periodontitis, and adult periodontitis. these species include actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, porphyromonas gingivalis, and prevotella intermedia. sev ...	1997	9037612
effect of in vitro aging on campylobacter rectus lipopolysaccharide-stimulated pge2 release from human gingival fibroblasts.	we examined the influence of in vitro aging on campylobacter rectus (c. rectus) lipopolysaccharide (lps)-stimulated prostaglandin (pg), e2 release from human gingival fibroblasts (hgfs).	1996	9081760
periodontal bacteria colonizing oral mucous membranes in edentulous patients wearing dental implants.	the purpose of this study was to investigate the prevalence of the microbiota on the oral mucosal surfaces and in the peri-implant pocket in edentulous subjects with a past history of periodontitis. twenty edentulous subjects wearing dental implants for at least one year and with a history of periodontitis participated in this study. clinical parameters were assessed (plaque, redness, swelling, peri-implant probing depth [pipd], and bleeding on probing) and microbiological samples were taken (or ...	1997	9100195
matrix metalloproteinases-1, -3 and -8 and myeloperoxidase in saliva of patients with human immunodeficiency virus infection.	human immunodeficiency virus (hiv)-seropositive patients have frequently severe gingival inflammation and/or attachment loss. in addition many infectious diseases affect their periodontium with varying clinical manifestations. matrix metalloproteinases seem to play a key role in physiological periodontal remodelling and pathological tissue destruction. the aim of the present study was to characterize the presence, molecular forms, cellular sources, activities, and relative amounts of fibroblast- ...	1996	9171509
periodontal findings in cohen syndrome with chronic neutropenia.	radiographic periodontal status and microbiological findings of periodontal pockets in subjects with cohen syndrome are presented in this report. this hereditary disorder causes mental retardation, and neutropenia is one feature of the syndrome. fifteen patients with cohen syndrome and 15 controls matched for age and sex and, as far as possible, according to the degree of mental retardation were examined. alveolar bone loss was evaluated from the panoramic radiographs. two subgingival samples we ...	1997	9182743
identification and antibiotic sensitivity of bacteria isolated from periapical lesions.	periradicular tissues from 28 refractory endodontic cases requiring surgical intervention were submitted for histological diagnosis and microbiological culture. bacteria isolated from these lesions were identified and then tested for their antibiotic sensitivity to a panel of common antibiotics. the periapical tissue specimens of 22 out of 28 lesions (79%) contained microorganisms. of the 22 cases showing positive growth cultures, 15 were polymicrobial and 7 were single species isolates. fifty-t ...	1997	9220741
subgingival microbiota in adult chinese: prevalence and relation to periodontal disease progression.	the "checkerboard" dna-dna hybridization technology was used to study the epidemiology of 18 microbial species associated with various states of periodontal health and disease, in a sample of 148 chinese subjects never exposed to systematic dental therapeutic intervention, aged 30 to 39 and 50 to 59 years. our aims were to: 1) describe the prevalence of these microorganisms; 2) correlate the microbiological and clinical profiles of the subjects; and 3) examine the association between the microbi ...	1997	9249637
contribution of il-1 beta to the enhancement of campylobacter rectus lipopolysaccharide-stimulated pge2 production in old gingival fibroblasts in vitro.	campylobacter rectus is associated with adult periodontitis. we previously reported that c. rectus lipopolysaccharide (lps)-stimulated prostaglandin e2 (pge2) production in old cells of human gingival fibroblasts (hgfs) is higher than that in young cells. the present study examined whether an enhancement of c. rectus lps-stimulated interleukin (il)-1 beta production in old hgfs contributed to the increased production of pge2. lps was prepared from c. rectus atcc33238. hgfs were established from ...	1997	9255759
beta-lactamase producing bacteria in adult periodontitis.	in 23 untreated adult periodontitis patients, the occurrence of beta-lactamase producing periodontal bacteria was determined. in addition to non-selective isolation media, selective isolation and growth of beta-lactamase positive subgingival bacterial species was carried out on blood agar plates supplemented with amoxicillin and plates with amoxicillin+clavulanic acid. porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, peptostreptococcus micros, fusobacterium ...	1997	9266340
evaluation of antibodies reactive with campylobacter jejuni in egyptian diarrhea patients.	serum and stool samples were collected from 128 individuals: 96 diarrhea patients and 32 apparently healthy controls. stool specimens were cultured for enteric bacterial pathogens, while sera were screened by enzyme-linked immunosorbent assay for campylobacter jejuni-reactive antibodies. of 28 diarrhea patients who demonstrated c. jejuni-reactive antibodies (titers, > 100), 14 were culture positive for this organism. the 32 healthy controls showed significantly lower antibody titers (p < 0.05) w ...	1997	9302201
recently described clinically important anaerobic bacteria: taxonomic aspects and update.	a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ...	1997	9310640
putative periodontal pathogens in subgingival plaque of young adults with and without early-onset periodontitis.	this study assessed the associations between putative periodontal pathogens and early-onset periodontitis (eop) in a population of 248 subjects, 13 to 19 years of age at baseline, derived from a representative sample of u.s. young adults. the subjects were selected based on the presence or absence of attachment loss at baseline. the attachment level was assessed clinically at baseline and at a 6-year follow-up examination, and the presence of 7 bacterial species was assessed at follow-up using d ...	1997	9358364
"checkerboard" versus culture: a comparison between two methods for identification of subgingival microbiota.	the present study compared the "checkerboard" dna-dna hybridization methodology with culture techniques for the analysis of the composition of the subgingival microbiota. 70 subjects, presenting with a variety of periodontal conditions, contributed with a total of 283 subgingival plaque samples analyzed with respect to the following species: porphyromonas gingivalis, prevotella intermedia/prevotella nigrescens, fusobacterium nucleatum, campylobacter rectus, eikenella corrodens, bacteroides forsy ...	1997	9395099
an immunohistochemical study on the localization of porphyromonas gingivalis, campylobacter rectus and actinomyces viscosus in human periodontal pockets.	the localization and distribution of porphyromonas gingivalis, campylobacter rectus and actinomyces viscosus were studied in human periodontal pockets. after obtaining voluntary consent from 9 patients, 12 teeth and their surrounding periodontal tissue with advanced adult periodontitis were extracted carefully so as not to change the structure of the periodontal pockets. the specimens were processed into serial sections. one of the sections was stained with brown & brenn-modified gram stain to o ...	1997	9401932
detection of porphyromonas gingivalis from saliva by pcr by using a simple sample-processing method.	simple sample-processing methods for pcr detection of porphyromonas gingivalis, a major pathogen causing adult periodontitis, from saliva were studied. the ability to detect p. gingivalis from 118 salivary samples by pcr after boiling and chelex 100 processing was compared with bacterial culture. p. gingivalis was detected three times more often by pcr than by culture. chelex 100 processing of saliva proved to be effective in preventing pcr inhibition and was applied to determine the occurrence ...	1998	9431940
periodontitis patient and the spouse: periodontal bacteria before and after treatment.	suspected periodontal pathogens can be transmitted between spouses. the treatment response may be unsuccessful in periodontitis patients, if the spouse harbors these bacteria. the aim of the present 6-month follow-up study was to clarify whether the microbiological treatment outcome of periodontitis patients is related to the detection of suspected periodontal pathogens in the saliva of the spouse. 10 patients with advanced periodontitis and their spouses were included in the study. the patients ...	1997	9442426
oral food consumption and subgingival microorganisms: subgingival microbiota of gastrostomy tube-fed children and healthy controls.	this study examined the effect of oral food consumption on the prevalence and levels of subgingival bacteria and yeasts in 20 gastrostomy tube-fed children and 24 healthy controls. microbial identification was carried out using anaerobic culture and 16s rrna-based pcr identification methods. streptococcal and actinomyces species were recovered from 100% and 76% of all subjects and averaged 66% and 11% of total cultivable organisms, respectively. in decreasing order of prevalence, fusobacterium, ...	1997	9444590
association of oral spirochetes from sites of periodontal health with development of periodontitis.	the purpose of this investigation was to determine whether the presence of disease-associated bacteria in health-associated plaque correlated with susceptibility to periodontitis over time. sites of periodontal health were identified in 65 adults. six months later (recall 1), plaque was collected from sites that remained in periodontal health, and specific bacteria were detected using monoclonal antibodies in a microscopic assay. the spirochete morphogroup was identified by phase contrast micros ...	1997	9444597
prevalence of six periodontal pathogens detected by dna probe method in hiv vs non-hiv periodontitis.	the aim of the study was to examine the prevalence of selected periodontal pathogens associated with hiv and non-hiv related periodontal lesions.	1997	9456680
gram-positive anaerobic cocci.	gram-positive anaerobic cocci (gpac) are a heterogeneous group of organisms defined by their morphological appearance and their inability to grow in the presence of oxygen; most clinical isolates are identified to species in the genus peptostreptococcus. gpac are part of the normal flora of all mucocutaneous surfaces and are often isolated from infections such as deep organ abscesses, obstetric and gynecological sepsis, and intraoral infections. they have been little studied for several reasons, ...	1998	9457430
design and evaluation of useful bacterium-specific pcr primers that amplify genes coding for bacterial 16s rrna.	we report the design and evaluation of pcr primers 63f and 1387r for amplification of 16s rrna genes from bacteria. their specificity and efficacy were tested systematically with a bacterial species and environmental samples. they were found to be more useful for 16s rrna gene amplification in ecological and systematic studies than pcr amplimers that are currently more generally used.	1998	9464425
role for the s-layer of campylobacter rectus atcc33238 in complement mediated killing and phagocytic killing by leukocytes from guinea pig and human peripheral blood.	the role of the s-layer of campylobacter rectus atcc 33238 in complement-mediated killing and interaction with leukocytes of the intraperitoneal cavity from guinea pigs and human peripheral blood was studied in vitro.	1997	9467352
evidence for serum immunoglobulin g (igg) antibody responses in macaca fascicularis identified by monoclonal antibodies to human igg subclasses.	this investigation determined the capacity of murine monoclonal antibodies directed to human immunoglobulin g (igg) subclasses to identify molecules with conserved epitopes in the serum of the nonhuman primate, macaca fascicularis. we subsequently utilized this cross-reactivity to document the characteristics of igg subclass antibody responses in m. fascicularis to parenteral immunization with intact oral microorganisms, antigens from oral microorganisms, and finally a defined protein toxin, tet ...	1997	9467387
characteristics of systemic antibody responses of nonhuman primates to cell envelope and cell wall antigens from periodontal pathogens.	the immune response of the primate, macaca fascicularis, to cell envelope (cea) or cell wall (cwa) antigens of several periodontal pathogens was examined to develop a strategy to interfere with ligature-induced periodontitis. animals were parenterally immunized with cea of either porphyromonas gingivalis, prevotella intermedia or a combination of cea/cwa of campylobacter rectus, fusobacterium nucleatum and actinomyces viscosus. serum samples were taken every 2-4 weeks over a 4-month period, whic ...	1997	9467388
microbiota of health, gingivitis, and initial periodontitis.	this study compared the subgingival microbiota in periodontal health, gingivitis and initial periodontitis using predominant culture and a dna probe, checkerboard hybridization method. 56 healthy adult subjects with minimal periodontal attachment loss were clinically monitored at 3-month intervals for 12 months. more sites demonstrated small increments of attachment loss than attachment gain over the monitoring period. sites, from 17 subjects, showing > or = 1.5 mm periodontal attachment loss du ...	1998	9495607
microbial complexes in subgingival plaque.	it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ...	1998	9495612
a new member of the s-layer protein family: characterization of the crs gene from campylobacter rectus.	strains of the periodontal pathogen campylobacter rectus express a 150- to 166-kda protein on their cell surface. this protein forms a paracrystalline lattice, called the surface layer (s-layer), on the outer membrane of this gram-negative bacterium. to initiate a genetic analysis of the function of the s-layer in the pathogenesis of c. rectus, we have cloned and characterized its gene. the s-layer gene (crs) from c. rectus 314 encodes a cell surface protein which does not have a cleaved signal ...	1998	9529076
intervening sequences in 16s rrna genes of campylobacter sp.: diversity of nucleotide sequences and uniformity of location.	we found and sequenced intervening sequences (ivss) in the pcr-amplicons of 16s rrna genes of 3 strains of campylobacter rectus, 2 strains of c. curvus and 2 strains of c. sputorum. the lengths of the ivss were 140 to 233 bp. the ivss of c. rectus were identical and had a sequence homology of 55 to 79% against those of c. curvus and c. helveticus. the ivss of c. sputorum were 97.9-100% homologous but poorly homologous to the other ivss. in spite of the diversities of the lengths and the nucleoti ...	1998	9570291
effect of red blood cells on the growth of porphyromonas endodontalis and microbial community development.	establishment of a microbial community in the root canal system depends on numerous factors, of which nutrient availability may be one of the most important. we hypothesized that the presence of red blood cells or hemoglobin in this environment could cause shifts in microbial composition of communities, resulting in organisms such as porphyromonas endodontalis becoming more dominant. an in vitro model system using mixed, batch cultures was performed with the bacteria p. endodontalis, fusobacteri ...	1998	9573801
identification of bacterial species on or in crevicular epithelial cells from healthy and periodontally diseased patients using dna-dna hybridization.	the purpose of this investigation was to identify bacterial species present on or in crevicular epithelial cells in healthy and diseased sites using dna probes. in order to achieve this aim, further improvements were made in the separation of unattached bacteria from those adherent to epithelial cells isolated from the human gingival crevice or periodontal pocket. then the dna probes were used to determine the prevalence of detectable dna from 15 microbial species on or in crevicular epithelial ...	1998	9573819
oral bone loss is increased in ovariectomized rats.	alveolar bone loss associated with periodontal disease occurs frequently in postmenopausal females, the same group that is predisposed to osteoporosis. to determine if the estrogen-deficient state enhances oral bone loss, we studied ovariectomized rats administered the potent bone-resorbing cytokine interleukin-1 or the periodontal pathogen campylobacter rectus lipopolysaccharide (lps). distal root canals of first mandibular molars were instrumented with endodontic files, and bone resorbing fact ...	1997	9587293
in-vitro antibacterial effects of extracts of nigerian tooth-cleaning sticks on periodontopathic bacteria.	aqueous extracts from 8 plants used for tooth-cleaning in nigeria were tested for their ability to inhibit the growth of five periodontopathic bacteria, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, eikenella corrodens and campylobacter rectus. extracts of all the plants except that of massularia acuminata exhibited varying growth inhibitory potentials on the microorganisms. extract of terminalia glaucescens showed the widest spectrum of activity, inhibiting the growt ...	1995	9590895
association of oral spirochetes from periodontally healthy sites with development of gingivitis.	the purpose of this investigation was to determine whether the presence of selected disease-associated bacteria in health-associated plaque correlated with future gingivitis. sites of periodontal health were identified in 65 adults. six months later (recall 1) plaque was collected from sites that remained in periodontal health, and 5 species of specific bacteria and pathogen-related oral spirochetes were detected using monoclonal antibodies in a microscopic assay. members of the spirochete morph ...	1998	9609381
the effect of herbal extracts in an experimental mouthrinse on established plaque and gingivitis.	the purpose of the present study was to establish in vitro the inhibiting effect of a herbal extract mixture on a selected number of micro-organisms and to test in vivo the effect of a mouthwash containing 6.3 mg/ml herbal extract mixture on plaque and gingivitis as compared to a minus active control mouthrinse. the herbal extract was a mixture of: juniperus communis (juniper), urtica dioca (nettle), achillaea millefolium (yarrow); 1:1:1. in the study, in-vitro, the effect of pure herbal extract ...	1998	9650877
severity of localized juvenile periodontitis as related to polymorphonuclear chemotaxis and specific microbial isolates.	the purpose of this study was twofold: (a) to determine if an association exists between severity of localized juvenile periodontitis (ljp) and impairment of polymorphonuclear leukocyte (pmn) chemotaxis and/or colonization by specific microbial isolates; (b) to determine if the number of specific microbial isolates, i.e., actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, eikenella corrodens, and campylobacter rectus correlates to clin ...	1998	9663117
campylobacter upsaliensis: waiting in the wings.	despite strong epidemiological evidence supporting an important role for campylobacter upsaliensis as a human enteropathogen, it remains relatively unknown in the realm of clinical microbiology. clinical studies indicate that infection with this organism usually is associated with benign self-limiting diarrhea. however, more serious illnesses, including spontaneous abortion and hemolytic-uremic syndrome, recently have been associated with human infections. understanding of the virulence properti ...	1998	9665977
bone resorption caused by three periodontal pathogens in vivo in mice is mediated in part by prostaglandin.	gingival inflammation, bacterial infection, alveolar bone destruction, and subsequent tooth loss are characteristic features of periodontal disease, but the precise mechanisms of bone loss are poorly understood. most animal models of the disease require injury to gingival tissues or teeth, and the effects of microorganisms are thus complicated by host responses to tissue destruction. to determine whether three putative periodontal pathogens, porphyromonas gingivalis, campylobacter rectus, and fu ...	1998	9712762
oral microbiota in smokers and non-smokers in natural and experimentally-induced gingivitis.	the present study primarily aimed at investigating the oral microbiota in smokers and non-smokers with established gingivitis and monitoring its composition during experimental gingivitis. secondly, it aimed at examining whether the composition of the microbiota is associated with different levels of gingival inflammation during this experimental gingivitis trial. for this purpose, 25 non-dental university students with gingivitis were recruited. 11 subjects were smokers and 14 were non-smokers. ...	1998	9722273
early-onset periodontitis associated with down's syndrome--clinical interventional study.	individuals with down's syndrome (ds) have an increased prevalence of periodontal disease compared with otherwise normal, age-matched control groups and other mentally handicapped patients of similar age distribution. the exaggerated immune-inflammatory response of the tissues cannot be explained by poor oral hygiene alone and might be the result of an impaired cell-mediated and humoral immunity and a deficient phagocytic system. as far as the progression and severity of destruction, the oral ma ...	1998	9722720
surface structure, hydrophobicity, phagocytosis, and adherence to matrix proteins of bacillus cereus cells with and without the crystalline surface protein layer.	nonopsonic phagocytosis of bacillus cereus by human polymorphonuclear leukocytes (pmns) with particular attention to bacterial surface properties and structure was studied. two reference strains (atcc 14579(t) and atcc 4342) and two clinical isolates (oh599 and oh600) from periodontal and endodontic infections were assessed for adherence to matrix proteins, such as type i collagen, fibronectin, laminin, and fibrinogen. one-day-old cultures of strains oh599 and oh600 were readily ingested by pmns ...	1998	9746594
identification and analyses of periodontal pathogens in taiwan by microbiological tests.	the purpose of this study was to use microbiological tests for diagnosis of periodontal diseases in taiwan. anaerobic culture, direct microscopy, indirect immunofluorescence (if), and biochemical tests were used to examine 336 samples for the specific microorganisms in subgingival plaque. the results indicated that gram-negative species and motile bacteria were less frequently detected, and in lower proportion, in samples from healthy sites. the bacteria found frequently in healthy group were th ...	1994	9747334
the s-layer protein from campylobacter rectus: sequence determination and function of the recombinant protein.	the gene encoding the crystalline surface layer (s-layer) protein from campylobacter rectus, designated slp, was sequenced and the recombinant gene product was expressed in escherichia coli. the gene consisted of 4086 nucleotides encoding a protein with 1361 amino acids. the n-terminal amino acid sequence revealed that slp did not contain a signal sequence, but that the initial methionine residue was processed. the deduced amino acid sequence displayed some common characteristic features of s-la ...	1998	9770285
the groel-like protein from campylobacter rectus: immunological characterization and interleukin-6 and -8 induction in human gingival fibroblast.	the native groel-like protein was purified from campylobacter rectus, a putative periodontal pathogen, by affinity chromatography on atp-agarose followed by high performance liquid chromatography on superose 6. the purified 64-kda protein (denatured form of groel-like protein) was immunoreactive by sds-page and western immunoblotting with the monoclonal antibody directed against heat shock protein 60 of human origin. the native groel-like protein stimulated both interleukin-6 (il-6) and il-8 sec ...	1998	9785445
subgingival temperature and microbiota in initial periodontitis.	the association between subgingival temperature, other clinical characteristics, and the subgingival microbiota was examined in adult subjects with initial periodontitis and differing levels of gingival inflammation. 43 subjects were measured at 6 sites per tooth for pocket depth, attachment level, presence of plaque, gingival redness, bleeding on probing and subgingival temperature at 3-month intervals for 1 year. subgingival plaque was sampled from 15 initial active periodontitis sites (10 sub ...	1998	9797050
relationship between c-telopeptide pyridinoline cross-links (ictp) and putative periodontal pathogens in periodontitis.	crevicular fluid pyridinoline cross-linked carboxyterminal telopeptide of type i collagen (ictp) is predictive for future alveolar bone loss in experimental periodontitis in dogs. the present study sought to relate ictp to a panel of subgingival species in subjects exhibiting various clinical presentations such as health (n=7), gingivitis (n=8) and periodontitis (n=21). 28 subgingival plaque and gcf samples were taken from mesiobuccal sites in each of 36 subjects. the presence and levels of 40 s ...	1998	9846794
campylobacter fetus surface layer proteins are transported by a type i secretion system.	the virulence of campylobacter fetus, a bacterial pathogen of ungulates and humans, is mediated in part by the presence of a paracrystalline surface layer (s-layer) that confers serum resistance. the subunits of the s-layer are s-layer proteins (slps) that are secreted in the absence of an n-terminal signal sequence and attach to either type a or b c. fetus lipopolysaccharide in a serospecific manner. antigenic variation of multiple slps (encoded by sapa homologs) of type a strain 23d occurs by ...	1998	9851986
rapid detection of the chlamydiaceae and other families in the order chlamydiales: three pcr tests.	few identification methods will rapidly or specifically detect all bacteria in the order chlamydiales, family chlamydiaceae. in this study, three pcr tests based on sequence data from over 48 chlamydial strains were developed for identification of these bacteria. two tests exclusively recognized the chlamydiaceae: a multiplex test targeting the ompa gene and the rrna intergenic spacer and a taqman test targeting the 23s ribosomal dna. the multiplex test was able to detect as few as 200 inclusion ...	1999	9986815
relationship of the subgingival microbiota to a chairside test for aspartate aminotransferase in gingival crevicular fluid.	the aim of the present study was to evaluate the association between the occurrence of certain specific periodontal pathogens and aspartate aminotransferase (ast) levels in gingival crevicular fluid (gcf).	1999	10052771
microbiota of successful osseointegrated dental implants.	the long-term survival of dental implants depends, in part, on control of bacterial infection in the peri-implant region. periodontal pathogens colonized implants symptomatic through infection, whereas the microbiota of successful implants was similar to that of periodontal health. this study examined the impact on the peri-implant microbiota of crown restorations; implant type; length of time of loading; history of implant or periodontal infections; and whether implants replaced single or multi ...	1999	10102550
cloning and characterization of two bistructural s-layer-rtx proteins from campylobacter rectus.	campylobacter rectus is an important periodontal pathogen in humans. a surface-layer (s-layer) protein and a cytotoxic activity have been characterized and are thought to be its major virulence factors. the cytotoxic activity was suggested to be due to a pore-forming protein toxin belonging to the rtx (repeats in the structural toxins) family. in the present work, two closely related genes, csxa and csxb (for c. rectus s-layer and rtx protein) were cloned from c. rectus and characterized. the cs ...	1999	10198015
detection of unculturable bacteria in periodontal health and disease by pcr.	recently developed molecular methods have made it possible to characterize mixed microflora in their entirety, including the substantial numbers of bacteria which do not grow on artificial culture media. in a previous study, molecular analysis of the microflora associated with acute oral infections resulted in the identification of three phylotypes, pus3.42, pus9.170, and pus9.180, representing as-yet-uncultured organisms. the aim of this study was to design and validate specific pcr primers for ...	1999	10203507
simultaneous detection of bacteroides forsythus and prevotella intermedia by 16s rrna gene-directed multiplex pcr.	in a 16s rrna gene-directed multiplex pcr, prevotella intermedia- and bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. a 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. a total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation.	1999	10203541
environmental modulation of oral treponeme virulence in a murine model.	this investigation examined the effects of environmental alteration on the virulence of the oral treponemes treponema denticola and treponema pectinovorum. the environmental effects were assessed by using a model of localized inflammatory abscesses in mice. in vitro growth of t. denticola and t. pectinovorum as a function of modification of the cysteine concentration significantly enhanced abscess formation and size. in contrast, growth of t. denticola or t. pectinovorum under iron-limiting cond ...	1999	10338481
fused and overlapping rpob and rpoc genes in helicobacters, campylobacters, and related bacteria.	the genes coding for the beta (rpob) and beta' (rpoc) subunits of rna polymerase are fused in the gastric pathogen helicobacter pylori but separate in other taxonomic groups. to better understand how the unique fused structure evolved, we determined dna sequences at and around the rpob-rpoc junction in 10 gastric and nongastric species of helicobacter and in members of the related genera wolinella, arcobacter, sulfurospirillum, and campylobacter. we found the fusion to be specific to helicobacte ...	1999	10368167
eikenella corrodens phase variation involves a posttranslational event in pilus formation.	the human pathogen eikenella corrodens synthesizes type iv pili and exhibits a phase variation involving the irreversible transition from piliated to nonpiliated variants. on solid medium, piliated variants form small (s-phase), corroding colonies whereas nonpiliated variants form large (l-phase), noncorroding colonies. we are studying the molecular basis of this phase variation in the clinical isolate e. corrodens va1. a genomic fragment encoding the major type iv pilin was cloned from the s-ph ...	1999	10400570
the treponema denticola major sheath protein is predominantly periplasmic and has only limited surface exposure.	the recent discovery that the treponema pallidum genome encodes 12 orthologs of the treponema denticola major sheath protein (msp) prompted us to reexamine the cellular location and topology of the t. denticola polypeptide. experiments initially were conducted to ascertain whether msp forms an array on or within the t. denticola outer membrane. transmission electron microscopy (em) of negatively stained and ultrathin-sectioned organisms failed to identify a typical surface layer, whereas freeze- ...	1999	10417176
porphyromonas gingivalis prevalence related to other micro-organisms in adult refractory periodontitis.	forty-six adult periodontal patients, selected on the basis of clinical examination, and 46 adult healthy subjects were examined. the subgingival plaque samples from one inflammatory and one non-inflammatory site of each periodontal patient were studied to determine porphyromonas gingivalis prevalence related to other periodontal micro-organisms and to periodontal tissue destruction. the results showed porphyromonas gingivalis as the main pathogenic micro-organism isolated in the inflammatory si ...	1999	10423739
periodontitis associated bacteria in supragingival plaque of dental hygienists: stability of carrier state and clinical development.	the purpose of this study was the clinical and microbiological re-examination of dental hygienists, who, 30 months before, had shown remarkably high supragingival levels of periodontitis-associated micro-organisms. interdental plaque was collected from the same molar sites and investigated by the same immunofluorescence assay with taxa-specific monoclonal antibodies as at the initial examination. on average, the 15 re-examined subjects showed slightly increased plaque levels but unchanged bleedi ...	1999	10424388
enhancement of lipopolysaccharide-stimulated pge2 and il-1beta production in gingival fibroblast cells from old rats.	the effect of aging on gingival fibroblasts in response to bacterial infection was studied. rat gingival fibroblast (rgf) cells were cultured from gingival tissue removed from young (6 weeks old) and old (20 months old) rats. both types of rgf cells were challenged with lipopolysaccharide (lps) from the periodontal pathogen campylobacter rectus. the levels of prostaglandin e2 (pge2) and interleukin 1beta (il-1beta) released into the cultured medium were measured by a specific radioimmunoassay. l ...	1999	10433392
the production of interleukin-1beta from human fetal membranes is not obligatory for increased prostaglandin output.	bacterial endotoxin increased the expression of mrna (maximal after 4 hr) for interleukin-1beta (il-1beta) and the release of mature protein from intact human fetal membranes. in contrast, the change in expression of mrna for type 2 cyclo-oxygenase (cox-2) was biphasic, with peaks after 0.5-1 hr and after 8 hr of culture. an antibody to il-1beta was without effect after 4 hr of culture, inhibited endotoxin-stimulated prostaglandin e2 (pge2) production after 8 hr of culture, and caused a parallel ...	1999	10447739
improved multiplex pcr using conserved and species-specific 16s rrna gene primers for simultaneous detection of actinobacillus actinomycetemcomitans, bacteroides forsythus, and porphyromonas gingivalis.	among putative periodontal pathogens, actinobacillus actinomycetemcomitans, bacteroides forsythus, and porphyromonas gingivalis are most convincingly implicated as etiological agents in periodontitis. therefore, techniques for detection of those three species would be of value. we previously published a description of a multiplex pcr that detects a. actinomycetemcomitans and p. gingivalis. the present paper presents an improvement on that technique, which now allows more sensitive detection of a ...	1999	10523542
identification by subtractive hybridization of a novel insertion sequence specific for virulent strains of porphyromonas gingivalis.	subtractive hybridization was employed to isolate specific genes from virulent porphyromonas gingivalis strains that are possibly related to abscess formation. the genomic dna from the virulent strain p. gingivalis w83 was subtracted with dna from the avirulent strain atcc 33277. three clones unique to strain w83 were isolated and sequenced. the cloned dna fragments were 885, 369, and 132 bp and had slight homology with only bacillus stearothermophilus is5377, which is a putative transposase. th ...	1999	10531208
lack of humoral immune protection against treponema denticola virulence in a murine model.	this study investigated the characteristics of humoral immune responses to treponema denticola following primary infection, reinfection, and active immunization, as well as immune protection in mice. primary infection with t. denticola induced a significant (400-fold) serum immunoglobulin g (igg) response compared to that in control uninfected mice. the igg response to reinfection was 20, 000-fold higher than that for control mice and 10-fold higher than that for primary infection. mice actively ...	1999	10531223
enhancement of lps-stimulated plasminogen activator production in aged gingival fibroblasts.	plasminogen activator (pa) converts plasminogen to plasmin, and plasmin activates the kinin cascade and latent methalloproteases. it is known that the alteration of the pa-plasmin system affects the progression of periodontal disease. we have reported previously that lps from campylobacter rectus, which is associated with adult periodontitis, increased pa production in human gingival fibroblasts (hgf). the effects of in vitro- and in vivo-cellular ageing on pa production from human and rat gingi ...	1999	10567948