Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| high-level expression of recombinant protein a in escherichia coli. | a high-level expression plasmid ppa-3 was constructed, which yielded up to 20% of soluble cell proteins as recombinant protein a in e. coli dh5 alpha strain by heat shock induction. the recombinant protein a contained only the five domains of native protein a that bind with the fc part of igg. the molecular weights of the recombinant protein a expressed in e. coli were determined to be 33kda, 32.2kda, 29.5kda and 28.6kda by sds-page and western-blotting. the diversity of the molecular weights ma ... | 1992 | 1297444 |
| a 4-kilobase congo red binding plasmid dna fragment of shigella dysenteriae 1 suppresses the growth and cell differentiation in escherichia coli. | a 4-kilobase congo red binding plasmid dna fragment of pcat 120 of shigella dysenteriae 1 was transferred to an escherichia coli k12 strain by transformation. transformants were unable to grow in any liquid broth medium. electron microscopic studies revealed that the transformants grown on tryptic soy agar were associated in clusters after cell division. normal cell separation among the transformants in comparison with recipient e. coli k12 was only observed when the growth medium was supplement ... | 1992 | 1307443 |
| adaptation of transmissible gastroenteritis virus to growth in non-permissive vero cells. | the cpk cells derived from swine kidney were infected with the attenuated to-163 strain of transmissible gastroenteritis (tge) virus, and fused with uninfected vero cells in the presence of polyethylene glycol. repeated cocultivation of the fused cells with uninfected vero cells rendered the virus to grow in vero cells. the vero cell-adapted virus acquired the ability to infect and produce cytopathic effects in several other non-permissive cell lines of non-porcine origin. no major differences i ... | 1992 | 1309640 |
| [laboratory breeding of the red-backed vole (clethrionomys rufocanus bedfordiae)]. | laboratory matings were attempted to establish breeding colonies of red-backed voles (clethrionomys rufocanus bedformidae) as experimental animals. for these mating, 10 pairs of red-backed voles which were captured in the tohbetsu region of hokkaido, japan and their litters were used. in the results for two years, 1987 to 1988, the rates of pregnancy, birth and weaning were 35.4%, 94.5% and 79.5%, respectively. the mean litter size was 5:1 +/- 1.6 with a range of 1 to 9. the mean gestation perio ... | 1992 | 1315690 |
| preservation of an inbred mouse strain by the storage of frozen embryos: an estimate of the time of occurrence of a serum igg1 deficiency mutation found in mrl-lpr. | 1992 | 1318459 | |
| effect of intestinal bacteria on the virulence of entamoeba histolytica. | the effect on amebal virulence of escherichia coli, clostridium symbiosum and a mixture of lactobacilli was investigated. amebas from hm1 and hk axenic strains were incubated with a single bacterial strain or lactobacilli for short, intermediate, or long periods and analyzed for their erythrophagocytosis, hemolytic activity and cytotoxicity in vitro and hepatic abscesses formation in vivo. it was found that in vitro virulence was enhanced by all studied bacteria in hk9 but not in hm1 strains, wh ... | 1992 | 1340289 |
| identification of the escherichia coli muri gene, which is required for the biosynthesis of d-glutamic acid, a specific component of bacterial peptidoglycan. | the muri gene of escherichia coli, whose inactivation results in the inability to form colonies in the absence of d-glutamic acid, was identified in the 90-min region of the chromosome. the complementation of an auxotrophic e. coli b/r strain by various dna sources allowed us to clone a 2.5-kbp ecori chromosomal fragment carrying the muri gene into multicopy plasmids. the muri gene corresponds to a previously sequenced open reading frame, orf1 (j. brosius, t. j. dull, d. d. sleeter, and h. f. no ... | 1992 | 1355768 |
| sequential infection of tsetse flies with trypanosoma congolense and trypanosoma brucei. | the question whether tsetse flies can be experimentally infected with more than one trypanosome species or strain by sequential feeding was investigated using dna probe technology to identify directly the small numbers of trypanosomes in the fly gut. bloodstream form trypanosomes of trypanosoma congolense or t. brucei ssp. were used for initial infection, followed by sequential feeds using either t. congolense or t. brucei ssp. midgut trypanosome populations were subsequently analysed by hybridi ... | 1992 | 1356306 |
| molecular characterization of cloned variants of coxiella burnetii isolated in china. | to study the molecular properties of coxiella burnetii phase variants we cloned the phase variants of c. burnetii qiyi (cbqy) strain by the red plaque technique. three cloned strains, cbqyic3 (phase i), cbqyiic7 (phase ii) and cbqyiic5 (semirough-phase) were analysed by sds-page, immunoblot assay, plasmid isolation and agarose gel electrophoresis of dna restriction fragments. the results suggest that the unique phase-dependent substance is a lipopolysaccharide and that most protein components of ... | 1992 | 1359769 |
| use of the escherichia coli ssb gene to prevent bioreactor takeover by plasmidless cells. | reactor takeover by plasmidless cells is a major problem encountered when producing proteins from plasmid-borne genes in genetically engineered bacteria. we have approached this problem by deleting the essential ssb gene from the escherichia coli chromosome and placing it on a plasmid. plasmidless cells do not accumulate even after growing such strains under non-selective continuous culture conditions for extended periods of time. other ssb-containing plasmids can be readily introduced into this ... | 1990 | 1366512 |
| purification and some properties of a thermostable metal proteinase produced by thermomicrobium sp. kn-22 strain. | an extreme thermophile that produces a heat-stable proteinase was isolated from hot-spring water and classified as thermomicrobium sp. kn-22 (growth temperature, 50-83 degrees c; and optimum growth temperature, 70 degrees c). the proteinase was purified from the culture broth of this strain by fractionation with ammonium sulfate, chromatography on columns of deae-cellulose and cm-sepharose cl-6b, and hplc on tskgel cm-5pw. the purified enzyme gave a single band on sds-polyacrylamide gel electrop ... | 1991 | 1368714 |
| involvement of recb-mediated (but not recf-mediated) repair of dna double-strand breaks in the gamma-radiation production of long deletions in escherichia coli. | experiments were designed to determine the association between the repair of gamma-radiation-induced dna double-strand breaks (dsb) and the induction of 700-1000 bp long deletions (lac(-)----lac+), base substitutions (leub19----leu+), and frameshifts (trpe9777----trp+) in escherichia coli k-12. over the range of 2.5-20 krad, deletions were induced with linear kinetics, as has been shown for the induction of dsb, while the induction kinetics of base substitutions and frameshifts were curvilinear. ... | 1992 | 1370245 |
| carbohydrate masking of an antigenic epitope of influenza virus haemagglutinin independent of oligosaccharide size. | comparison of the haemagglutinins (ha) of the pathogenic avian influenza viruses a/fpv/dutch/27 (h7n7) and a/fpv/rostock/34 (h7n1) revealed 94.7% nucleotide and 93.8% amino acid sequence homologies. six of the seven n-glycosidic oligosaccharides of the rostock ha are at the same positions as the six carbohydrates of the dutch strain. the additional oligosaccharide side chain of the rostock strain, which is of the complex type, is attached to asparagine149 in antigenic epitope b. the accessibilit ... | 1992 | 1379858 |
| cloning and sequencing of influenza c/yamagata/1/88 virus ns gene. | it was previously shown that the shortest rna of influenza c/california/78 virus contains 934 nucleotides and codes for two nonstructural proteins of 286 amino acids (ns 1) and 121 amino acids (ns 2). in this report, we determined the nucleotide sequence of the ns gene of the recently isolated influenza c/yamagata/1/88 strain by using cloned cdna derived from the viral rna. compared with the ns gene of c/california/78, one nucleotide insertion has occurred in the ns gene of c/yamagata/1/88. this ... | 1992 | 1388016 |
| glisoprenins, new inhibitors of acyl-coa: cholesterol acyltransferase produced by gliocladium sp. fo-1513. i. production, isolation and physico-chemical and biological properties. | gliocladium sp. fo-1513 was found to produce novel inhibitors of acyl-coa: cholesterol acyltransferase (acat). two active compounds, designated glisoprenins a and b, were isolated from the culture broth of the producing strain by a conventional method. the ic50 values of glisoprenins a and b for acat activity were 46 and 61 microm in an enzyme assay using rat liver microsomes, and 1.2 and 0.57 microm in a j774 macrophage assay, respectively. | 1992 | 1399839 |
| [acanthamoeba keratitis. morphologic and isoenzymatic study of a strain isolated from a medically treated case]. | we report here the third case of acanthamoeba keratitis discovered in france in a 30 year-old man wearing soft contact lenses. amoebas of acanthamoeba genus could be isolated twice from his corneal ulcer with simple ocular swabs. owing to medical treatment alone, a successful healing of his eye was obtained. the isolated strain was studied morphologically and biochemically: the cysts observed microscopically after silver stain possessed less than six arms surrounded by a non reticulated ectocyst ... | 1992 | 1422274 |
| a comparison of the pathogenicity of two strains of hog cholera virus. 1. clinical and pathological studies. | the virulence of a strain of hog cholera virus isolated during an outbreak of mild disease in pigs in new south wales in 1960/61 (the nsw strain) was compared over 11 days with that of a virulent strain by inoculating 8 pigs with each virus and comparing the ensuing clinical signs and pathology. both viruses caused persistent pyrexia and leukopenia, the nsw strain 4 to 5 days and the virulent strain 3 days, after inoculation. few other clinical signs were observed in the pigs inoculated with the ... | 1992 | 1445070 |
| involvement of the escherichia coli rna polymerase alpha subunit in transcriptional activation by the bacteriophage lambda ci and cii proteins. | escherichia coli cells harbouring the rpoa341 mutation produce an rna polymerase which transcribes inefficiently certain operons subject to positive control. here, we demonstrate that the rpoa341 allele also prevents lysogenization of the host strain by bacteriophage lambda, a process dependent upon the action of two phage-encoded activators. this phenomenon was shown to arise from an inability to establish an integrated prophage rather than a failure to maintain the lysogenic state. the inabili ... | 1992 | 1452017 |
| purification and characterization of the hydantoin racemase of pseudomonas sp. strain ns671 expressed in escherichia coli. | the hydantoin racemase gene of pseudomonas sp. strain ns671 had been cloned and expressed in escherichia coli. hydantoin racemase was purified from the cell extract of the e. coli strain by phenyl-sepharose, deae-sephacel, and sephadex g-200 chromatographies. the purified enzyme had an apparent molecular mass of 32 kda as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. by gel filtration, a molecular mass of about 190 kda was found, suggesting that the native enzyme is a ... | 1992 | 1459947 |
| interaction between flocculent and nonflocculent cells of saccharomyces cerevisiae. | interaction between nonflocculent and flocculent cells of saccharomyces cerevisiae was studied. adhesion experiments were done using three types of nonflocculent cells and a flocculent one. two types of nonflocculent cells were obtained from the flocculent strain by changing environmental growth conditions. the integration of nonflocculent cells in the flocs was observed by two different methods: measurement of the sedimentation capacity of mixtures and microscopic observation of stained nonfloc ... | 1992 | 1464069 |
| translocation of a folded protein across the outer membrane in escherichia coli. | a mutation in the escherichia coli dsba gene (coding for a periplasmic disulfide oxidoreductase) reduces the rate of disulfide bond formation in the enzyme pullulanase and also reduces the rate at which the enzyme is secreted to the cell surface, as measured by protease accessibility. the enzyme did not become protease accessible when disulfide bond formation was completely prevented in the mutant strain by carboxymethylation. these results indicate that a disulfide bond may be required for, and ... | 1992 | 1465440 |
| attempted transmission of borrelia burgdorferi (spirochaetales: spirochaetaceae) (jdi strain) by ixodes scapularis (acari: ixodidae), dermacentor variabilis, and amblyomma americanum. | laboratory-reared ixodes scapularis say, amblyomma americanum (l.), and dermacentor variabilis (say) were fed on new zealand white rabbits experimentally infected with borrelia burgdorferi (jdi strain). at repletion, spirochetes could be detected by dark-field microscopy only in i. scapularis. acquisition rates were 18 and 21%. when previously exposed nymphs of each species were fed on susceptible rabbits, i. scapularis was the only tick of the three species that transmitted b. burgdorferi. when ... | 1992 | 1495078 |
| safety and immunogenicity of a booster dose of vibrio cholerae cvd 103-hgr live oral cholera vaccine in swiss adults. | adult volunteers received a booster dose (4 x 10(8) cfu) of attenuated vibrio cholerae cvd 103-hgr oral vaccine 15 or 24 months after primary immunization. the immune response was modest, presumably due to rapid clearance of the vaccine strain by a primed immune system. | 1992 | 1500200 |
| effect of synergists on the oral and topical toxicity of azamethiphos to organophosphate-resistant houseflies (diptera: muscidae). | dermal and oral toxicities of azamethiphos were determined in two organophosphate-resistant and one susceptible strain of houseflies, musca domestica l. the 594vb strain was 1,967-fold more resistant to azamethiphos when compared with the susceptible chemical specialties manufacturers association (csma) strain by dermal application. when the compound was administered orally to the 594vb strain, we observed only a 15-fold resistance. in contrast, the yachiyo strain, which show 1,500-fold resistan ... | 1992 | 1517504 |
| effect of number of hens per nipple waterer on the performance of several strains of layers in cages. | two experiments were designed to study the effects of housing in cages with several hens per nipple waterer (hpn) ratios on performance of several strains of white leghorn pullets. in experiment 1, only body weight gain and water consumption were significantly affected by the hpn; hens at the 2:1 hpn gained more weight and consumed more water per day than those at the 4:1 hpn. the lack of a significant strain by hpn interaction indicated that the four strains responded similarly to the different ... | 1992 | 1523178 |
| lipopolysaccharide heterogeneity in escherichia coli j5 variants: analysis by flow cytometry. | a lipopolysaccharide o-side chain-producing phenotypic variant was isolated from a uridine 5'-diphosphogalactose 4-epimeraseless escherichia coli j5 rough mutant strain by fluorescence-activated cell sorting with a monoclonal antibody (mab) specific for the o-side chain of e. coli o111:b4 smooth parent lipopolysaccharide. the variant (j5-2) was recognized by both core- and o-side chain-specific mabs, while the "original" rough mutant (j5-1) and smooth parent strains stained only with core- and o ... | 1992 | 1527415 |
| significance of ventricular arrhythmias in systemic hypertension with left ventricular hypertrophy. | hypertensive patients with the electrocardiographic (ecg) pattern of left ventricular (lv) hypertrophy and strain are at increased risk of sudden death. it has been suggested that ventricular arrhythmias may be responsible. the prevalence and significance of ventricular arrhythmias was therefore studied in 90 hypertensive patients with lv hypertrophy and strain by undertaking 48-hour ambulatory ecg monitoring, ecg signal-averaging and programmed ventricular stimulation. complex ventricular ectop ... | 1992 | 1532285 |
| [osteoporotic fractures of the spine in elderly women. risk factors, inducing factors and course]. | forty-seven women with osteoporotic fractures of the spine were examined consecutively. the age range was 64-91 years with a median of 77 years. risk factors for osteoporosis such as induced menopause or gastric resection were not present. four patients had had rheumatoid arthritis. 19% had been treated with glucocorticoid. only one patient had received long-term oestrogen treatment. the patients had been physically very active at a younger age and 91% had been occupationally employed. the sympt ... | 1992 | 1539391 |
| growth inhibition of francisella tularensis live vaccine strain by ifn-gamma-activated macrophages is mediated by reactive nitrogen intermediates derived from l-arginine metabolism. | we have examined the abilities of the recombinant murine lymphokines ifn-gamma, granulocyte-macrophage (gm)-csf, and il-4 to stimulate the in vitro antimicrobial activity of macrophages against the live vaccine strain (lvs) of francisella tularensis. resident peritoneal macrophages from c57bl/6 strain mice were cultured overnight with ifn-gamma, gm-csf, or il-4, and then infected with lvs. in macrophages treated with ifn-gamma, the growth of lvs was suppressed by a factor of 100- to 1000-fold in ... | 1992 | 1541823 |
| replacement of the folc gene, encoding folylpolyglutamate synthetase-dihydrofolate synthetase in escherichia coli, with genes mutagenized in vitro. | the folylpolyglutamate synthetase-dihydrofolate synthetase gene (folc) in escherichia coli was deleted from the bacterial chromosome and replaced by a selectable kmr marker. the deletion strain required a complementing gene expressing folylpolyglutamate synthetase encoded on a plasmid for viability, indicating that folc is an essential gene in e. coli. the complementing folc gene was cloned into the vector ppm103 (psc101, temperature sensitive for replication), which segregated spontaneously at ... | 1992 | 1548226 |
| detailed histopathological examination contributes to the assessment of escherichia coli urovirulence. | to determine the usefulness of detailed histopathological evaluation in the assessment of urovirulence of different escherichia coli strains in a mouse model of ascending, unobstructed urinary tract infection, and to evaluate the relationship between susceptibility to urinary tract infection and renal levels of p fimbrial receptor glycolipids in different mouse strains, female swiss webster and balb/c mice were inoculated transurethrally with one of four different well-characterized wild type e. ... | 1992 | 1552614 |
| nyvac: a highly attenuated strain of vaccinia virus. | a highly attenuated vaccinia virus strain, nyvac (vp866), was derived from a plaque-cloned isolate of the copenhagen vaccine strain by the precise deletion of 18 open reading frames (orfs) from the viral genome. among the orfs deleted from nyvac (vp866) are two genes involved in nucleotide metabolism, the thymidine kinase (orf j2r) and the large subunit of the ribonucleotide reductase (orf i4l); the gene encoding the viral hemagglutinin (orf a56r); the remnant (orf a26l) of a highly expressed ge ... | 1992 | 1566575 |
| cloning and overproduction of biodegradative threonine deaminase from escherichia coli w strain. | we have cloned the structural gene (tdcb) of biodegradative threonine deaminase from escherichia coli w strain by utilizing the polymerase chain reaction. the jm109/puctda strain, which was obtained by transforming e. coli jm109 with a vector plasmid (puctda) containing the cloned tdcb gene, produced a large amount of the enzyme corresponding to more than 5% of the total soluble protein. amino acid sequence analysis of this recombinant enzyme showed that the amino acid sequence is identical to t ... | 1992 | 1586456 |
| overexpression of the wild-type gene coding for escherichia coli dna adenine methylase (dam). | the gene coding for escherichia coli dam methylase was isolated from a dam+ k12 strain by the pcr method. the gene was subcloned into an overexpression vector under the control of the strong lambda pl promoter. the resultant construct produced the dam methylase at about 20% of total cellular protein. purification of the protein was achieved with two chromatography columns and yielded 6 mg of pure methylase per gram cell paste. the methylase readily methylates the synthetic dodecamer gactgatcagtc ... | 1992 | 1590763 |
| inactivation of wild-type and rad mutant caenorhabditis elegans by 8-methoxypsoralen and near ultraviolet radiation. | survival of wild-type and four radiation-sensitive (rad) mutants of the nematode caenorhabditis elegans was determined after near-uv irradiation in the presence of 8-methoxypsoralen (8-mop). three sets of inactivation profiles were generated for each strain by irradiating synchronous populations of either early embryos, late embryos or first-stage larvae (l1s). late embryos were consistently the most sensitive. curiously, none of the four rad mutants were even moderately hypersensitive. split-do ... | 1992 | 1603841 |
| sequential nuclear magnetic resonance assignment of beta-1,2-linked mannooligosaccharides isolated from the phosphomannan of the pathogenic yeast candida albicans nih b-792 strain. | the h-1 and h-2 signals of beta-1,2-linked mannooligosaccharides isolated from the phosphomannan of candida albicans nih b-792 strain by mild acid hydrolysis were assigned by a sequential nmr assignment method that combines two-dimensional 1h-1h correlated spectroscopy (cosy) and two-dimensional nuclear overhauser enhancement and exchange spectroscopy (noesy). the results indicated that the h-1 and h-2 of each beta-1,2-linked mannopyranose unit show largely different signals compared with those ... | 1992 | 1610816 |
| sequence analysis of five new field isolates demonstrates that the chain length of potato spindle tuber viroid (pstvd) is not strictly conserved but as variable as in other viroids. | the sequence analysis of five new field isolates of potato spindle tuber viroid (pstvd) of different virulence revealed that the length of their rna chain is not strictly conserved to 359 nucleotides (nts), as one could have inferred from the previously sequenced pstvd strains. it was now found that the chain length is strain-specific like in the case of practically all other viroids, and that it may vary, so far, between 356 and 360 nts. taking our previously sequenced and least virulent mild s ... | 1992 | 1623184 |
| construction of a multivalent meningococcal vaccine strain based on the class 1 outer membrane protein. | outer membrane complexes (omcs) are promising vaccine candidates for protection against meningococcal disease. however, a major obstacle to this approach is the fact that the protective antibodies induced are generally type specific. in an attempt to overcome this problem, we have investigated the possibility of constructing a multivalent vaccine strain by insertion of an additional class 1 outer membrane protein-encoding gene. starting with a derivative of strain h44/76 deficient in class 3 out ... | 1992 | 1639486 |
| the petite phenotype resulting from a truncated copy of subunit 6 results from loss of assembly of the cytochrome bc1 complex and can be suppressed by overexpression of subunit 9. | disruption of the gene for subunit 6 of the yeast cytochrome bc1 complex (qcr6) causes a temperature-sensitive petite phenotype in contrast to deletion of the coding region of qcr6, which shows no growth defect. mitochondria from the petite strain carrying the disruption allele were devoid of ubiquinol-cytochrome c oxidoreductase activity but retained cytochrome c oxidase and oligomycin-sensitive atpase activities. optical spectra of cytochromes in mitochondrial membranes from the petite strain ... | 1991 | 1651316 |
| detection and identification of vaccine-related polioviruses by the polymerase chain reaction. | we have used the polymerase chain reaction (pcr) to obtain sensitive detection and identification of poliovirus rna genomes. primer pairs were designed to permit identification of each sabin poliovaccine strain by the electrophoretic mobilities of the amplified dna products (sabin 1: 97 bp; sabin 2: 71 bp; sabin 3: 44 bp). the compositions of samples containing mixtures of vaccine strains could be readily determined by pcr. when the amplified products were visualized by ethidium bromide fluoresc ... | 1991 | 1659060 |
| genetic analysis of the antifungal activity of a soilborne pseudomonas aureofaciens strain. | pseudomonas aureofaciens q2-87 produces the antibiotic 2,4-diacetophloroglucinol (phl), which inhibits gaeumannomyces graminis var. tritici and other fungi in vitro. strain q2-87 also provides biological control of take-all, a root disease of wheat caused by this fungus. to assess the role of phl in the antifungal activity of strain q2-87, a genetic analysis of antibiotic production was conducted. two mutants of q2-87 with altered antifungal activity were isolated by site-directed mutagenesis wi ... | 1991 | 1660695 |
| sensitivity of the standardized pseudorabies virus neutralization test varies with the test strain used. | the effect of altering the strain of the test virus used in the standardized pseudorabies virus neutralization (vn) test on the sensitivity of the assay was evaluated. comparative vn tests were performed using 4 different strains: the avirulent bartha parental, the avirulent recombinant bartha giiika, the moderately virulent shope (currently used for the vn test at the national veterinary services laboratory, ames, ia), and the highly virulent p2208 (funkhauser). a radioimmunoassay and a western ... | 1991 | 1662079 |
| characterization of an avian rotavirus strain by neutralization and molecular hybridization assays. | pigeon rotavirus strain po-13, which was recently shown to be neutralized by a hyperimmune serum to the prototype serotype 7 virus ch-2, showed a one-way neutralization cross with turkey rotavirus ty-1. when its genome was compared by rna-rna hybridization under stringent conditions with those of avian and mammalian rotaviruses, po-13 displayed a low to medium level of homology only with turkey rotavirus strains ty-1 and ty-3 but not with chicken rotavirus strain ch-1. furthermore, no homology w ... | 1991 | 1665575 |
| outbreak of paralytic poliomyelitis in oman: evidence for widespread transmission among fully vaccinated children. | from january, 1988, to march, 1989, a widespread outbreak (118 cases) of poliomyelitis type 1 occurred in oman. incidence of paralytic disease was highest in children younger than 2 years (87/100,000) despite an immunisation programme that recently had raised coverage with 3 doses of oral poliovirus vaccine (opv) among 12-month-old children from 67% to 87%. we did a case-control study (70 case-patients, 692 age-matched controls) to estimate the clinical efficacy of opv, assessed the immunogenici ... | 1991 | 1679866 |
| thy 1 expression in the brain of nude mice. | the expression and cell distribution of thy 1 antigen was studied in the brain of both normal and athymic (nude) young adult mice of the balb/c strain by immunochemistry. in nude animals thy 1 fluorescence was less intense and less regularly distributed in the molecular layer of the cerebellum and hippocampus. thy 1 content determined by elisa was lower by 10-16% in the cerebellum and 20-25% in the olfactory bulbs of nude mice. the total wet weight of the brain was lower by 16% than in control a ... | 1991 | 1681895 |
| experimental studies to determine the susceptibility to infection with st. louis encephalitis virus of five species of panamanian mosquitoes. | the susceptibility to infection with a panamanian isolate of st. louis encephalitis (sle) virus was evaluated in culex quinquefasciatus, haemagogus equinus, mansonia dyari, sabethes cyaneus and deinocerites pseudes. when fed on blood-virus suspensions using the hanging drop or pledget technique, the median infective dose (id50) of sle virus for colonized strains of cx. quinquefasciatus and hg. equinus was 10(2.8) plaque forming units (pfu)/2 microliters and 10(3.5) pfu/2 microliters, respectivel ... | 1991 | 1686273 |
| epitopes of escherichia coli alpha-hemolysin: identification of monoclonal antibodies that prevent hemolysis. | the antigenic regions of escherichia coli alpha-hemolysin were determined by antibody binding to cyanogen bromide (cnbr) fragments of this protein under denatured conditions. alpha-hemolysin was isolated from filtered culture supernatants of a recombinant strain by a combination of trichloroacetic acid precipitation and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. alpha-hemolysin was used to (i) produce polyclonal rabbit antisera and murine monoclonal immunoglobulin g ( ... | 1990 | 1696938 |
| modification of the t cell responsiveness to synthetic peptides by substituting amino acids on agretopes. | t cell receptors, major histocompatibility complex molecules, and antigens constitute tri-molecular complexes which induce t cell activation. t cells in i-ab mice generate proliferative responses to a synthetic peptide composed of residues 43-58 of pigeon cytochrome c (p43-58) and its analogs with substitution at position 50 (50a, 50v, 50l, 50n, 50q, 50k, and 50m). however, none of these peptides stimulate t cells in i-ak mice. we substituted two residues at positions 46 and 54 of p43-58(50d), 5 ... | 1990 | 1708277 |
| comparative analysis of bacillus anthracis, bacillus cereus, and related species on the basis of reverse transcriptase sequencing of 16s rrna. | the primary structures of the 16s rrnas of bacillus anthracis, bacillus cereus, bacillus mycoides, and bacillus thuringiensis were determined by using the reverse transcription-dideoxy sequencing method. all of the strains exhibited very high levels of sequence similarity (greater than 99%) that were consistent with the close relationships shown by previous dna hybridization studies. the sequences of b. anthracis sterne and b. cereus emetic strain nctc 11143 were found to be identical for a cont ... | 1991 | 1715736 |
| antigenic variation in giardia lamblia: infection of congenitally athymic nude and scid mice. | athymic nude mice of the outbred zur:icr-nu and inbred balb/c strain and scid mice were infected with a cloned human isolate of giardia lamblia (gs/m-83-h7). changes in the expression of the major surface epitope of the intestinal trophozoites (characterized by the binding capacity of monoclonal antibody moabg10/4) as well as cellular and humoral immune parameters of the hosts were followed during the course of infection. self-cure was observed in heterozygous (nu/+) balb/c mice by day 22 post-i ... | 1991 | 1725820 |
| human brucellosis: immunoblotting analysis of three brucella abortus antigenic fractions allows the detection of components of diagnostic importance. | results indicating that analysis of the immune humoral response of brucellosis patients by immunoblotting provides useful information for the characterization of antigenic fractions of possible diagnostic importance in human brucellosis are presented. sera of 90 patients were obtained: 23 suffering from chronic brucellosis, 20 from acute brucellosis and 47 belonging to the group of serologically positive individuals (spi) without clinical evidence of active infection at the time of examination, ... | 1991 | 1726568 |
| role of trat protein, an anticomplementary protein produced in escherichia coli by r100 factor, in serum resistance. | escherichia coli k12 strain w3110/sm bearing a plasmid containing the trat gene (trat+ strain) was more resistant to the bactericidal activity of guinea pig serum than the same strain bearing this plasmid without the trat gene (trat- strain). a murine mab was generated against synthetic trat peptide (86-99). this antibody reacted only with denatured trat protein, but it was used for monitoring trat protein by immunoblotting during purification of the protein. six mab were then generated against ... | 1992 | 1730875 |
| purification and biochemical characterisation of the ecor124 type i modification methylase. | large scale purification of the type i modification methylase ecor124 has been achieved from an over-expressing strain by a two step procedure using ion-exchange and heparin chromatography. pure methylase is obtained at a yield of 30 mg per gm of cell paste. measurements of the molecular weight and subunit stoichiometry show that the enzyme is a trimeric complex of 162 kda consisting of two subunits of hsdm (58 kda) and one subunit of hsds (46 kda). the purified enzyme can methylate a dna fragme ... | 1992 | 1741244 |
| [cloning and inactivation of a chromosomal copy of the imidazole glycerophosphate dehydratase (his) gene from hansenula polymorpha]. | a gene for imidazole glycerophosphate dehydratase (his) has been selected from the library of hansenula polymorpha genes by complementation of escherichia coli hisb mutations or his3 mutation of saccharomyces cerevisiae. inactivation of the gene in hansenula polymorpha strain 48v, as well as leu(-)-leu+ conversion of phenotype and arising of antibiotic g418 resistance resulted from transformation of the strain by the linear dna molecules of the cloned his-gene with the in vitro inserted leu2 gen ... | 1991 | 1745264 |
| generation of aromatic-dependent salmonella havana and evaluation of its immunogenic potential in mice and sheep. | the generation of aromatic-dependent (aro-) salmonella havana (group g2, 01, 13, 23) from a smooth wild-type parent strain by transduction with phage p1 is reported. mice immunized with this live aro- s. havana strain (cs234) by the intraperitoneal (i.p.) route were protected against challenge with wild-type s. havana, whereas those immunized by the oral route were not. mice immunized with two doses of formalin-killed aro- s. havana by the i.p. route were also unprotected, in spite of high antib ... | 1991 | 1746157 |
| purpactins, new inhibitors of acyl-coa:cholesterol acyltransferase produced by penicillium purpurogenum. i. production, isolation and physico-chemical and biological properties. | penicillium purpurogenum fo-608, a soil isolate, was found to produce a series of new inhibitors of acyl-coa:cholesterol acyltransferase (acat). three active compounds, designated purpactins, a, b and c, were isolated from the fermentation broth of the producing strain by solvent extraction, silica gel column chromatography and hplc. purpactins inhibit acat activity in an enzyme assay system using rat liver microsomes with ic50 values of 121 approximately 126 microm. purpactin a also inhibited c ... | 1991 | 1750931 |
| age- and sex-related differences in the nerve growth factor distribution in the rat brain. | levels of the nerve growth factor (ngf) have been measured in various brain regions of young and aged male and female rats of wistar strain by means of a highly sensitive two-site enzyme immunoassay system for beta-ngf. among the ten regions examined, the amount of ngf per wet weight of tissue was found to be highest in the hippocampus, irrespective of the sex and age. the ngf concentration in the hippocampus of female rats at 3 months of age was comparable to that of same aged males. further, t ... | 1991 | 1756386 |
| skin tearing in broilers in relation to skin collagen: effect of sex, strain, and diet. | the relationship between skin tearing and collagen in broilers was investigated in two trials in which strain and sex, and strain and diet served as factorial-arranged variables, respectively. in the first trial, males and females of three strains were examined. both skin tearing and skin collagen were significantly influenced by strain and sex without any significant strain by sex interaction. skin collagen, expressed as a fraction of fresh skin protein (n x 6.25) was lower and skin tearing was ... | 1991 | 1780263 |
| a quantitative measurement of the effect of avian influenza virus on the ability of turkeys to eliminate pasteurella multocida from the respiratory tract. | the effect of avian influenza virus (aiv) infection on the ability of turkeys to eliminate pasteurella multocida from the respiratory tract was evaluated. four-week-old turkeys were experimentally infected with an apathogenic aiv subtype (h5n2) by the oculonasal route and subsequently superinfected with p multocida (urbach strain) by the intranasal route three days after infection with aiv. quantitative clearance of p multocida from the trachea and lung was determined using a pour plate techniqu ... | 1991 | 1780577 |
| relevance of inoculation route to virulence of three salmonella spp. strains in mice. | the virulence of three salmonella species strains was compared by the i.p. and i.v. routes in balb/c mice. salmonella choleraesuis, sl2824 (serogroup c1, o-6,7), was more virulent by the i.v. than i.p. route. a strain of s. typhimurium, sl1260 (serogroup b; o-1,4,12) was more virulent i.p. than i.v. while another strain, sl3201 (o-4,5,12) was equally virulent i.p. or i.v. the ld50 of each strain by either route correlated with the number of bacteria in the liver and spleen on day one after inocu ... | 1991 | 1795625 |
| elevated paraquat resistance can be used as a bioassay for longevity in a genetically based long-lived strain of drosophila. | a long-lived (l) strain of drosophila melanogaster, derived from a normal-lived (r) strain by artificial selection, has a significantly different adult longevity. previous work has shown that 1) the two strains age in the same manner, 2) the major genes responsible for much of the l strain's extended longevity are located on the 3rd chromosome, and 3) the extended longevity phenotype is significantly modulated by the larval environment. in this report, we investigate the resistance of the l and ... | 1991 | 1806332 |
| characterization of neurotoxigenic clostridium butyricum strain by dna hybridization test and by in vivo and in vitro germination tests of spores. | the germination of spores of a neurotoxigenic clostridium butyricum strain (bl 6340), which was isolated from infant botulism in italy, and that of a nontoxigenic c. butyricum type strain (ncib 7423) were studied. the spores of bl 6340 strain were killed at 80 c for 10 min, and required the mixture of l-alanine, l-lactate, glucose and bicarbonate for their optimal germination. these characteristics are the same as those of clostridium botulinum type e strain, but different from those of ncib 742 ... | 1991 | 1808463 |
| opsonic effect of c-reactive protein on phagocytosis and intracellular killing of virulent and attenuated strains of candida albicans by human neutrophils. | in the presence of autologous complement, maximal phagocytosis of candida albicans blastospores of both a virulent and attenuated strain by human neutrophils in a monolayer assay was achieved after 30 min. the proportion of phagocytes containing intracellular blastospores was 33-36% with an average of 1.5 blastospores per phagocyte. in contrast to the attenuated strain of c. albicans, the virulent strain resisted opsonization by c-reactive protein (crp) and of those blastospores ingested, only 8 ... | 1991 | 1812935 |
| siderophore production by aeromonas salmonicida. | growth under conditions of iron-restriction and the production of siderophores was examined in 21 typical and 14 atypical strains of aeromonas salmonicida. with the exception of one atypical strain, all strains grew and multiplied in the presence of the high-affinity iron chelators ethylenediamine di(o-hydroxyphenylacetic acid), alpha, alpha'-dipyridyl or transferrin. chrome azurol s agar was used to screen bacterial strains growing under these conditions for the production of siderophores. side ... | 1991 | 1830896 |
| inbred rat strain comparisons indicate different sites of action for cocaine and amphetamine locomotor stimulant effects. | cocaine and amphetamine produce several behavioral effects, most notably locomotor stimulation. biochemically, evidence suggests specific involvement of dopaminergic systems, although not necessarily identical sites, in mediating cocaine- and amphetamine-induced locomotor stimulation. this study examined the effects of cocaine or amphetamine on locomotor activity in rats from the aci, f344, lew and nbr inbred strains. dose-dependent increases in locomotor activity were found for both drugs in al ... | 1991 | 1838200 |
| cloning, sequencing, and expression of the gene encoding the high-molecular-weight cytochrome c from desulfovibrio vulgaris hildenborough. | by using a synthetic deoxyoligonucleotide probe designed to recognize the structural gene for cytochrome cc3 from desulfovibrio vulgaris hildenborough, a 3.7-kb xhoi genomic dna fragment containing the cc3 gene was isolated. the gene encodes a precursor polypeptide of 58.9 kda, with an nh2-terminal signal sequence of 31 residues. the mature polypeptide (55.7 kda) has 16 heme binding sites of the form c-x-x-c-h. covalent binding of heme to these 16 sites gives a holoprotein of 65.5 kda with prope ... | 1991 | 1846136 |
| the complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and rna polymerase. | the 5'-most gene, gene 1, of the genome of murine coronavirus, mouse hepatitis virus (mhv), is presumed to encode the viral rna-dependent rna polymerase. we have determined the complete sequence of this gene of the jhm strain by cdna cloning and sequencing. the total length of this gene is 21,798 nucleotides long, which includes two overlapping, large open reading frames. the first open reading frame, orf 1a, is 4488 amino acids long. the second open reading frame, orf 1b, overlaps orf 1a for 75 ... | 1991 | 1846489 |
| characterization of three agrobacterium tumefaciens avirulent mutants with chromosomal mutations that affect induction of vir genes. | three agrobacterium tumefaciens mutants with chromosomal mutations that affect bacterial virulence were isolated by transposon mutagenesis. two of the mutants were avirulent on all hosts tested. the third mutant, ivr-211, was a host range mutant which was avirulent on bryophyllum diagremontiana, nicotiana tabacum, n. debneyi, n. glauca, and daucus carota but was virulent on zinnia elegans and lycopersicon esculentum (tomato). that the mutant phenotype was due to the transposon insertion was dete ... | 1991 | 1846853 |
| pif1: a dna helicase in yeast mitochondria. | the pif1 gene is involved in repair and recombination of mitochondrial dna (mtdna). in this study, the pif1 gene product, which cannot be identified in normal yeast cells, has been overproduced from the gali promoter to detectable protein levels. location of pif1 in mitochondria has been shown by immunoelectron microscopy and in vivo import experiments using ts mas1 mutants deficient in the mitochondrial matrix-localized processing protease. overproduction of pif1 protein in pif1 mutants restore ... | 1991 | 1849081 |
| isolation, characterization, and complementation of a paralyzed flagellar mutant of rhodobacter sphaeroides ws8. | a paralyzed rhodobacter sphaeroides mutant strain (para1) was isolated by a motility screening procedure following mutagenesis of wild-type r. sphaeroides ws8-n with the transposable element tnphoa (tn5 is50l::phoa). para1 synthesized a wild-type level of flagellin, as detected by western immunoblotting with antiflagellar antiserum. flagellar staining showed that flagellin was assembled into apparently normal external flagellar filaments. electron micrographs of basal body structures from para1 ... | 1991 | 1850401 |
| virulence phenotype and genetic characteristics of the t32-istrati shigella flexneri 2a vaccine strain. | the t32-istrati strain, which has been used as an oral attenuated shigella flexneri 2a vaccine, has lost the invasive phenotype due to a spontaneous deletion in the shigella virulence plasmid. this deletion has eliminated three plasmid loci (ipabcda, inva and virg) that are necessary for production of a positive sereny test by shigella species. virulence in the sereny test was reconstituted in the t32-istrati strain by the conjugal transfer of an intact 140 m da virulence plasmid from s. flexner ... | 1991 | 1872021 |
| diastolic anisotropic properties of the left ventricle in the conscious dog. | the role of myocardial anisotropy in determining change in left ventricular shape during diastolic filling has not yet been demonstrated. therefore, 11 conscious dogs were instrumented with global ultrasonic dimension transducers to measure left ventricular major and minor axis diameters and equatorial wall thickness. myocardial geometry was represented as a three-dimensional ellipsoidal shell. left ventricular transmural pressure was measured with micromanometers, and ventricular volume was var ... | 1991 | 1873871 |
| methods for detecting arctic rabies virus in dilution series of field samples. | a modified first passage cell culture method, mouse inoculation test, and elisa were compared for the detection of rabies in serially diluted field samples. each original specimen contained rabies antigen, individually identified as an arctic strain by monoclonal antibodies. in 47% of the samples tested in cell cultures, the end-point titer was increased as a result of the first passage. the cell culture method gave higher titers than the mouse inoculation test or elisa (p less than 0.05 and p l ... | 1991 | 1874913 |
| venezuelan equine encephalomyelitis virus infection in and transmission by the tick amblyomma cajennense (arachnida: ixodidae). | to assess a possible role of ticks as the maintenance host for epizootic strains of venezuelan equine encephalomyelitis (vee) virus, laboratory experiments were conducted to determine if ticks could become infected, maintain, and transmit the virus. larval and nymphal amblyomma cajennense (f.) and larval dermacentor nitens neumann ticks were exposed to epizootic vee virus (trinidad donkey strain) by allowing them to feed on viremic guinea pigs (strain 13). in a. cajennense, transstadial transmis ... | 1991 | 1875367 |
| synthesis of the virus-specified tubules of epizootic haemorrhagic disease virus using a baculovirus expression system. | the formation of virus-specific tubules is one of the most characteristic features in the orbivirus infection cycle, yet little is known about their role in virus replication. the tubuli are composed of a major nonstructural protein, ns1. we have investigated the expression of the ns1-encoding gene of epizootic haemorrhagic disease virus serotype 2 (alberta-strain) by producing a recombinant autographa californica nuclear polyhedrosis virus (acnpv). prior to cloning in the baculovirus transfer v ... | 1991 | 1891959 |
| comparison of in vitro replication and cytopathology caused by strains of canine distemper virus of vaccine and field origin. | three biological properties of canine distemper virus were examined to determine if any would consistently differentiate field from vaccine strains of the virus. the properties were the ability to (1) infect macrophages and epithelial cells, (2) produce distinct cytopathologic effect in alveolar and peritoneal macrophages and vero cells, and (3) produce pocks on the chorioallantoic membrane of embryonated chicken eggs. four vaccine strains and 5 field isolates were used in the study. the 5 field ... | 1991 | 1892930 |
| purification and some properties of pseudomonas fluorescens lipase. | lipase (triacylglycerol lipase, ec 3.1.1.3) has been purified from pseudomonas fluorescens wild strain by chromatography on deae-cellulose and octyl-sepharose cl-4b. the yield was 21% and the specific activity of the purified enzyme 4780 u/mg protein. it showed a mr of about 45 x 10(4) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the enzyme is active over a wide ph range and at 50-55 degrees c. | 1991 | 1905137 |
| comparative characterization of inducible and virulent vibrio parahaemolyticus bacteriophages having unique head projections. | phage tp1, induced from vibrio parahaemolyticus k-20 pilot strain by mitomycin c, exhibited a unique hexagonal head with knob-like projections which covered the whole capsid and a noncontractile tail. the appearance of this phage was very similar to those of phages vp3 and vp6, isolated from seawater. the host range of phage tp1 was similar to those of phages vp3 and vp6. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of the three phage particles revealed almost identical p ... | 1991 | 1908040 |
| adp/atp translocator is essential only for anaerobic growth of yeast saccharomyces cerevisiae. | all three genes (aac1, aac2 and aac3) encoding the mitochondrial adp/atp translocator, were inactivated in a haploid yeast strain by a gene disruption technique. the triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the aac2 gene was disrupted. it was further shown that a functional aac genes product is essential only for a ... | 1991 | 1915842 |
| structural and functional dissection of a membrane glycoprotein required for vesicle budding from the endoplasmic reticulum. | sec12p is a membrane glycoprotein required for the formation of a vesicular intermediate in protein transport from the endoplasmic reticulum to the golgi apparatus in saccharomyces cerevisiae. comparison of the n-linked glycosylation of sec12p, a sec12p-invertase hybrid protein, and a derivative of sec12p lacking 71 carboxy-terminal amino acids showed that sec12p is a type ii membrane protein. analysis of two truncated forms of sec12p and of a temperature-sensitive mutant indicated that the c-te ... | 1991 | 1922074 |
| structural study on a phosphorylated mannotetraose obtained from the phosphomannan of candida albicans nih b-792 strain by acetolysis. | a mixture of phosphorylated manno-oligosaccharides was isolated from the acid-stable domain of phosphomannan of candida albicans nih b-792 strain (serotype b) by acetolysis and was fractionated on a column of bio-gel p-2 equilibrated with 50 mm pyridine-ch3cooh buffer, ph 5.0. a monophosphorylated mannotetraose was isolated as the major constituent. structural analyses of this phosphate-containing tetraose and its reduction product with nabh4 by 1h, 13c, and two-dimensional homonuclear hartmann- ... | 1991 | 1929420 |
| [levels of type 1 polioviruses in food in an experimental model]. | the author concentrated from contamined foodstuffs poliovirus type 1--vaccine strain--by means of negative pressure or overpressure filtration across membranes with a different porosity and by means of an ultracentrifuge. foods - milk, meat, meat products and vegetables were contaminated by the virus so that various doses corresponded approximately to 1.2 or 4 pfu per gram of specimen. the virus in concentrates was detected on cell cultures on the vero line by the plaque method. the virus extrac ... | 1990 | 1964630 |
| recurrent lesions in human leishmania braziliensis infection--reactivation or reinfection? | strains of leishmania braziliensis subspecies isolated from initial and recurrent lesions in 24 patients from the pacific coast of colombia were examined for distinguishing polymorphisms by enzyme electrophoresis, restriction endonuclease analysis of kdna, and molecular karyotyping of nuclear dna. recurrent strains from 12 patients (50%) were identical to the initially infecting strain by all methods of characterisation. phenotypic and genotypic identity, together with clinical data, support end ... | 1990 | 1974943 |
| genetic manipulation of salmonella serotype bovismorbificans to aromatic-dependence and evaluation of its vaccine potential in mice. | the generation of smooth aromatic-dependent salmonella serotype bovismorbificans (group c2, o6, 8) from a smooth wild-type parent strain by transduction with phage p1, and conjugation with salmonella serotype typhimurium carrying f'-8gal is described. the smooth aromatic-dependent s. serotype bovismorbificans was non-lethal for mice at an oral challenge dose of 2 x 10(9) cfu (equivalent to 200 ld50 of the parent, wild-type strain). the safety of the auxotrophic mutant was further substantiated b ... | 1991 | 1990138 |
| virulence of campylobacter jejuni for chicken embryos is associated with decreased bloodstream clearance and resistance to phagocytosis. | the 11-day-old chicken embryo has been shown to be a useful animal model for comparing the virulence of human isolates of campylobacter jejuni. virulence in this system is associated with the ability to invade the chorioallantoic membrane and to survive and proliferate in vivo. in this study, the survival and multiplication of c. jejuni in the embryonic host was investigated. it was possible to enhance the virulence of a relatively avirulent c. jejuni strain by passaging it intravenously through ... | 1991 | 2004823 |
| direct effect of increased hemodynamic load on cardiac mass. | the hearts of rats were transplanted into the abdomens of recipients of the same inbred strain by attaching the stumps of aorta and pulmonary artery end to side to the abdominal aorta and inferior vena cava, respectively, of the recipient. the transplant functions as a denervated "nonworking" langendorff heart; the recipient in situ heart serves as a normal control. one week after surgery, an onset of atrophy is observed in the transplanted heart, which stabilizes after 2 weeks; the in situ hear ... | 1991 | 2009614 |
| [perceived exertion during simulated static work]. | the purpose of this study was to determine the effect of static work on the rating of perceived exertion (rpe). the subject were healthy, relatively fit young adults (16 men). exercise was performed at 25% mvc both in a commonly-used work posture and in a non-typical working posture (overhead-work). estimates of effort were recorded using the cr-20-scale by borg. a nonparametric analysis was carried out to examine the relationship between the level of static load and rpe. the results show that t ... | 1991 | 2028676 |
| fema, which encodes a factor essential for expression of methicillin resistance, affects glycine content of peptidoglycan in methicillin-resistant and methicillin-susceptible staphylococcus aureus strains. | fema is a chromosomally encoded factor, occurring naturally in staphylococcus aureus, which is essential for the expression of high-level methicillin resistance in this organism. the production of a low-affinity penicillin-binding protein, pbp2a or pbp2', which is intimately involved with methicillin resistance in s. aureus, is not influenced by fema. to elucidate a possible physiological function of the 48-kda protein encoded by fema, several related methicillin-resistant, methicillin-susceptib ... | 1991 | 2045371 |
| legionella adelaidensis, a new species isolated from cooling tower water. | a legionella-like organism (strain 1762-aus-e) was isolated from a cooling tower of an air-conditioning system in adelaide, south australia, australia. the isolate was presumptively identified as a legionella strain by its growth requirement for l-cysteine and its cellular branched-chain fatty acids. strain 1762-aus-e was serologically distinct in the slide agglutination test with absorbed antisera. dna hybridization confirmed that it is a new legionella species for which the name legionella ade ... | 1991 | 2056032 |
| surface properties of staphylococcus aureus affecting chemiluminescence response of human phagocytes. | to assess the surface properties of staphylococcus aureus affecting the response of human phagocytes, the effects of the organisms with different surface properties on the chemiluminescence (cl) response of human phagocytes were examined. the magnitude of the phagocytic cl response to hydrophobic strains was significantly greater than that to hydrophilic strains, while no significant difference in the cl response was seen between protein a-deficient strains and their parent strains. the cl respo ... | 1990 | 2077366 |
| use of a heterotopic cardiac isotransplant for pharmacological and toxicological studies. | the heterotopically transplanted rat heart provides a unique model for examination of the direct humoral effects on the myocardium since the transplanted heart is exposed to the same hormonal milieu as the in situ heart but does not support the hemodynamic load. in this model, the heart of an inbred rat is transplanted into the abdomen of a recipient of the same inbred strain by attaching the stumps of aorta and pulmonary artery end to side to the abdominal aorta and inferior vena cava of the re ... | 1990 | 2091232 |
| [wolff's law of transformation after 100 years]. | in the introduction to the article the author briefly describes the history of the wolff's invention that under the influence of the function the bone structure is restructuring. this functional adaptation however, has not been fully clarified until these days. at present the general scheme of the fundamental adaptation mechanism has been generally adopted according to which the bone responds to the inner strain by aposition of the new bone while inactivity results in resorption. the author's ow ... | 1990 | 2091427 |
| [study on the exoerythrocytic forms of plasmodium yoelii yoelii in rats and mice]. | sd rats and three strains of mice were infected with plasmodium yoelii yoelii by265 strain by intravenous inoculation of sporozoites via tail vein. liver tissues were taken 42 hours after infection and serial sections were made and stained by colophonium-giemsa method for microscopic examination. the ratio of the average value of major diameter/minor diameter of exoerythrocytic(ee)schizonts of plasmodium yoelii yoelii in rats and mice of icr/jcl, c57bl, km strains was 35.81 +/- 4.56 microns/29.7 ... | 1990 | 2099260 |
| identification of the cbp1 polypeptide in mitochondrial extracts from saccharomyces cerevisiae. | cbp1 is a nuclearly encoded yeast protein required for stability of mitochondrial cytochrome b pre-mrna. previous studies have shown that cbp1 stabilizes the cytochrome b transcripts via interaction with the 5'-end. for the present study, both rabbit polyclonal and mouse monoclonal antibodies against cbp1 were prepared using a trpe-cbp1 fusion polypeptide as a source of antigen. cbp1 was undetectable in a crude mitochondrial fraction from a wild-type strain by western blot assay, but a 66-kda im ... | 1990 | 2104848 |
| immunologic characterization of a 35-kilodalton recombinant antigen of mycobacterium tuberculosis. | a 35-kilodalton (kda) recombinant antigen (35-kda antigen) produced by escherichia coli jm107 carrying dna from mycobacterium tuberculosis was purified and immunologically examined by in vivo and in vitro methods. a monoclonal antibody (2b2) was produced against the 35-kda antigen. the protein was purified from the insoluble fraction of the recombinant e. coli strain by either affinity chromatography with the 2b2 monoclonal antibody or preparative isoelectric focusing. in enzyme-linked immunosor ... | 1990 | 2108996 |
| use of mixed islets from two strains as the donor for single transplantation. induction of donor-specific unresponsiveness to each strain by cyclosporine and intraportal grafting. | 1990 | 2113729 | |
| [spore-spheroplast transformation of bacillus megaterium]. | a new method for transformation of bacillus megaterium was developed by modification of chang and cohen's method. in our method, spore spheroplasts were used as recipient cells instead of the protoplasts of vegetative cells. longer incubation (60 min) of spore spheroplasts and plasmid dna before treatment with polyethylene glycol remarkably increased the efficiency of transformation. the frequency of transformation was about 10(4) per microgram of plasmid dna. a shot-gun-type cloning of chromoso ... | 1990 | 2125086 |
| tolytoxin and new scytophycins from three species of scytonema. | tolytoxin [1], the major cytotoxin associated with scytonema mirabile strain by-8-1, scytonema burmanicum strain do-4-1 and scytonema ocellatum strains dd-8-1, ff-65-1, and ff-66-3, has been shown to be 6-hydroxy-7-o-methylscytophycin b. minor amounts of three other new cytotoxic scytophycins, 6-hydroxyscytophycin b [2], 19-o-demethylscytophycin c [3], and 6-hydroxy-7-o-methylscytophycin e [4], have also been isolated from these cyanophytes. the gross structures and stereochemistry are based on ... | 1990 | 2128517 |
| point mutations in cauliflower mosaic virus gene vi confer host-specific symptom changes. | mutants of cauliflower mosaic virus (camv) strain d4 have been characterized with regard to host-specific phenotypes that resulted from specific changes in the viral dna sequence. both the mutant and the wild-type viruses infect a brassicaceous host, brassica campestris, systemically, giving indistinguishable symptoms. however, in the solanaceous host datura stramonium, which was systemically infectible by the wild-type virus, mutants induced necrotic local lesions at 21 degrees c and above, and ... | 2007 | 2134858 |
| diversity and stability of restriction enzyme profiles of plasmid dna from methicillin-resistant staphylococcus aureus. | nosocomial infections caused by methicillin-resistant staphylococcus aureus (mrsa) are a significant epidemiological problem. detecting the sources of epidemic strains and preventing their access to patients, however, depend upon the availability of techniques to reliably distinguish among mrsa strains. we evaluated restriction enzyme analysis of plasmid dna for use as an epidemiological marker of mrsa strains. the diversity of plasmid types was assessed by examining 120 clinical and environment ... | 1990 | 2153702 |