Publications

toxocariasis/cysticercosis seroprevalence in a long-term rural settlement, são paulo, brazil.	seroprevalence of toxocara and taenia solium and risk factors for infection with these parasites were explored in a long-term rural settlement in são paulo state, brazil. an elisa for the detection of anti-toxocara igg and ige and anti-t. solium cysticerci was standardized using toxocara excretory-secretory antigens (tes) obtained from the cultured second-stage larvae of t. canis and by vesicular fluid antigen from taenia crassiceps cysticerci (vf). for cysticercosis, the reactive elisa samples ...	2009	19366477
evidence of leishmania spp. antibodies and dna in bush dogs (speothos venaticus) in brazil.	the municipality of ilha solteira, são paulo, brazil is an endemic area of leishmaniasis. at the companhia energética de são paulo (cesp) wild animal center of ilha solteira, two bush dogs (speothos venaticus) showed clinical signs of this disease. the amastigote form of leishmania was detected in lymph-node smears taken by fine-needle biopsy. in addition, serum samples from both animals, screened with an enzyme-linked immunosorbent assay (elisa), were positive for anti-leishmania antibodies. mo ...	2009	19368245
tesa-blot for the diagnosis of chagas disease in dogs from co-endemic regions for trypanosoma cruzi, trypanosoma evansi and leishmania chagasi.	we standardized serodiagnosis of dogs infected with trypanosoma cruzi using tesa (trypomastigote excreted-secreted antigen)-blot developed for human chagas disease. tesa-blot showed 100% sensitivity and specificity. in contrast, elisa using tesa (tesa-elisa) or epimastigotes (epi-elisa) as antigen yielded 100% sensitivity but specificity of 94.1% and 49.4%, respectively. when used in field studies in an endemic region for chagas disease, visceral leishmaniasis and trypanosoma evansi (mato grosso ...	2009	19426657
evaluation of dna damage by the alkaline comet assay of the olfactory and respiratory epithelia of dogs from the city of são paulo, brazil.	animals kept as pets may be considered sentinels for environmental factors to which humans could be exposed. olfactory and respiratory epithelia are directly subjected to airborne factors, which could cause dna lesions, and the alkaline comet assay is considered a reliable tool for the assessment of dna damage. the objective of this work is to evaluate the extent of dna damage by the comet assay of the olfactory and respiratory epithelia of dogs from different regions of the city of são paulo, b ...	2010	19447591
[upgrading course for animal control agents: a new perspective on the zoonosis control services in the state of são paulo, southeastern brazil].		2009	19448918
babesia spp. infection in dogs from rural areas of são paulo state, brazil.	the status of babesia spp. infection in dogs from rural areas of são paulo state, brazil was studied. for this, 150 animals were examined by blood smears and by pcr; the presence of tick infestation was also investigated. by the blood smear examination, 3 animals (2%) were detected positive and by pcr for babesia spp. 12 (8%) were positive, with bands visualized in 450 bp. rhipicephalus sanguineus or amblyomma spp. were found on 36 (24%) of the 150 dogs. amblyomma species found were a. cajennens ...	2009	19602312
cutaneous pythiosis in a dog from brazil.	this report describes a case of cutaneous pythiosis in a 6-year-old female mixed breed dog, from the central west region of são paulo state, brazil. the cytological and histopathological analyses showed an intense inflammatory infiltrate with presence of numerous hyphal elements, suggesting infection due to pythium insidiosum. the diagnosis was confirmed by nested-pcr, which was carried out with specific primers derived from the ribosomal dna region. the pathogen occurs in brazil and veterinaria ...	2010	19706006
[ehrlichia canis in dogs attended in a veterinary hospital from botucatu, são paulo state, brazil].	this study investigated the etiology of canine ehrlichiosis and possible clinical and epidemiological data associated with the infection in 70 dogs suspect of ehrlichiosis attended at the veterinary hospital of the são paulo state university in botucatu city during 2001 and 2002. dogs were evaluated by clinical-epidemiological and hematological data and molecular analysis by partial amplification and dna sequencing of the ehrlichial dsb gene. e. canis dna was amplified and sequenced in 28 (40.0% ...	2009	19772777
toxoplasma gondii and leishmania spp. infection in captive crab-eating foxes, cerdocyon thous (carnivora, canidae) from brazil.	the zoonoses toxoplasmosis and leishmaniasis are important worldwide and also affect wild animals. thus, the present study aimed to assess the prevalence of leishmania spp. and toxoplasma gondii antibodies in 52 serum samples from captive crab-eating foxes (cerdocyon thous) kept in 17 zoos in são paulo state, brazil. modified agglutination test (mat, for toxoplasmosis) and indirect fluorescent antibody test (ifat, for toxoplasmosis and leishmaniasis) were employed with heterologous anti-dog immu ...	2010	20060648
prevalence of von willebrand disease in dogs from sao paulo state, brazil.	the aims of the current study were to assess the prevalence of von willebrand disease (vwd) in dogs from the region of botucatu, são paulo state, brazil, and to evaluate laboratory tests to diagnose this disease. the study included 350 dogs of various ages, different breeds, and both sexes. dogs included in the study had no historical or clinical evidence of abnormal bleeding. von willebrand factor antigen (vwfag), buccal mucosal bleeding time, activated partial thromboplastin time, and factor v ...	2010	20093683
diminished cd4+/cd25+ t cell and increased ifn-gamma levels occur in dogs vaccinated with leishmune in an endemic area for visceral leishmaniasis.	the leishmune vaccine has been used in endemic areas to prevent canine visceral leishmaniasis in brazil, but cytokine production induced by vaccination has rarely been investigated in dogs. this study aimed to evaluate the immune response of dogs vaccinated with leishmune fml vaccine (fort dodge) against total antigen of leishmania (leishmania) chagasi (tag) and fml. twenty healthy dogs from araçatuba, são paulo, brazil, an endemic leishmaniasis area, received three consecutive subcutaneous inje ...	2010	20132994
relationship between dog culling and incidence of human visceral leishmaniasis in an endemic area.	domestic dogs are the main reservoirs of visceral leishmaniasis (vl) in urban areas; one of the control measures adopted in brazil is the elimination of this reservoir. in order to test the relationship between the euthanasia rate of the canine reservoir and the incidence of the disease in humans, data on dog culling from the centre for zoonosis control of araçatuba, são paulo, brazil, during the period from 1999 to 2008 and visceral leishmaniasis human cases registered in the same period were a ...	2010	20181428
[characteristics of biting animals submitted to rabies diagnosis, são paulo state, brazil, 1993-2007].	epidemiological factors related to animal bites in humans and other animals were obtained from a database with 10,616 records of animal specimens sent for rabies diagnosis in northwest são paulo state, brazil, from 1993 to 2007. of this total, 61.5% contained information on the bites, and among the biting animals (25%), the majority were dogs (67%), followed by cats (21.8%), and bats (8.1%). in 92.1% of the reports the victims were humans, and 82.3% of the animals were home pets. the majority of ...	2009	20191165
human toxocariasis: contribution by brazilian researchers.	in the present paper the main aspects of the natural history of human infection by toxocara larvae that occasionally result in the occurrence of visceral and/or ocular larva migrans syndrome were reviewed. the contribution by brazilian researchers was emphasized, especially the staff of the tropical medicine institute of são paulo (imt).	2009	20209265
search for mycobacterium leprae in wild mammals.	leprosy is still a worldwide public health problem. brazil and india show the highest prevalence rates of the disease. natural infection of armadillos dasypus novemcinctus with mycobacterium leprae has been reported in some regions of the united states. identification of bacilli is difficult, particularly due to its inability to grow in vitro. the use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. in the present study, the diagnostic metho ...	2010	20428654
study of sand fly fauna in an endemic area of american cutaneous leishmaniasis and canine visceral leishmaniasis in the municipality of espírito santo do pinhal, são paulo, brazil.	canine american visceral leishmaniasis and american cutaneous leishmaniasis (acl) cases have been recorded in espírito santo do pinhal. the aim of this study was to gather knowledge of the sand fly community and its population ecology within the municipality. captures were made weekly over a period of 15 months in the urban, periurban and rural areas of the municipality, using automatic light traps. a total of 5,562 sand flies were collected, comprising 17 species. the most abundant species were ...	2010	20428683
survey of ticks (acari: ixodidae) and their rickettsia in an atlantic rain forest reserve in the state of são paulo, brazil.	the current study investigated the occurrence of ticks and their rickettsiae in the serra do mar state park, which encompasses one of the largest atlantic rain forest reserves of brazil. from july 2008 to june 2009, a total of 2439 ticks (2,196 free living and 243 collected on hosts) was collected, encompassing the following 13 species: amblyomma aureolatum (pallas), amblyomma brasiliense aragao, amblyomma dubitatum neumann, amblyomma fuscum neumann, amblyomma incisum neumann, amblyomma longiros ...	2010	20939390
antibodies to toxoplasma gondii and neospora caninum in captive neotropical and exotic wild canids and felids.	this study was designed to detect antibodies to toxoplasma gondii and neospora caninum in wild captive carnivores maintained in brazilian zoos. blood samples were collected from 142 brazilian wild felids and 19 exotic felids in zoos, and 3 european wolves (canis lupus) and 94 brazilian wild canids maintained in captivity in brazilian zoos of são paulo, mato grosso states and federal district. one hundred and two (63.4%) and 70 (50.3%) of the 161 wild felids tested were seropositive for t. gondii ...	2010	20950109
dna extraction from hair shafts of wild brazilian felids and canids.	wild felids and canids are usually the main predators in the food chains where they dwell and are almost invisible to behavior and ecology researchers. due to their grooming behavior, they tend to swallow shed hair, which shows up in the feces. dna found in hair shafts can be used in molecular studies that can unravel, for instance, genetic variability, reproductive mode and family structure, and in some species, it is even possible to estimate migration and dispersion rates in given populations ...	2010	21174262
occurrence and molecular characterization of cryptosporidium spp. isolated from domestic animals in a rural area surrounding atlantic dry forest fragments in teodoro sampaio municipality, state of são paulo, brazil.	the aim of this study was to assess the occurrence of cryptosporidium in domestic animals in rural properties surrounding rain forest fragments within the municipality of teodoro sampaio, southeastern brazil. conventional sucrose flotation method followed by molecular characterization of the parasites by sequencing pcr products amplified from ssu rrna gene were used. stool samples were collected from domestic animals raised as pets and livestock in all rural properties surrounding three forest f ...	2010	21184703
use of crude, fml and rk39 antigens in elisa to detect anti-leishmania spp. antibodies in felis catus.	visceral leishmaniasis is a disease caused by leishmania (leishmania) chagasi and represents a serious public health problem. the dog is the main urban reservoir of the disease; however, investigations regarding the occurrence and epidemiological importance of leishmaniasis in cats have recently been initiated. this study aimed to detect cats seropositive for leishmania spp. using different antigens. additional studies were performed using sera from cats with toxoplasma gondii (n=15) to evaluate ...	2010	21195550
a survey of owner attitudes and experiences in managing diabetic dogs.	a survey of the experiences of owners of diabetic dogs was carried out by distributing questionnaires to veterinary clinics in s+úo paulo, brazil. a total of 93 dog owners were surveyed over a 4month period and descriptive statistical analysis, frequency distribution and correlations were performed. in most cases the clinical condition of the dogs had improved following treatment and owners were largely satisfied with veterinary intervention. injection of neutral protamine hagedorm twice daily w ...	2011	21396860
protective and risk factors for toxocariasis in children from two different social classes of brazil.	the aim of this study was to analyze the prevalence of toxocara spp. antibodies in children from two different socioeconomic classes in the presidente prudente municipality, são paulo state, brazil, and the protective and risk factors associated with toxocariasis. one hundred and twenty-six middle-class (mc) and 126 disadvantaged children (dc) were included in this study. anti-toxocara elisa test was performed in order to evaluate seroprevalence. a survey was applied to the children's guardians/ ...	2011	21537752
[soil contamination by eggs of soil-transmitted helminths with zoonotic potential in the town of fernandópolis, state of são paulo, brazil, between 2007 and 2008.]	introduction: the concentration of dogs and cats in urban areas, associated with an ever-increasing wandering population of these animals, has an important epidemiological role in the soil contamination of public spaces and the spread of infections of several types of parasites. this study aimed to determine the frequency of soil-transmitted helminths with zoonotic potential in public squares and municipal primary schools in fernandópolis, state of são paulo, brazil, conducted between 2007 and 2 ...	2011	21552740
molecular characterization of giardia duodenalis in dogs from brazil.	the intestinal protozoan parasite giardia duodenalis (syn. giardia intestinalis and giardia lamblia) is a widespread enteric pathogen in human and domestic animals. this organism is one of the most common parasites in domestic dogs in brazil. in this study, we determined the occurrence and genetic characterization of g. duodenalis isolated from dogs from south-central são paulo state, brazil. a total of 300 fecal samples were collected. fecal specimens were screened for the presence of g. duoden ...	2011	21695567
giardiasis as zoonosis: between proof of principle and paradigm in the northwestern region of são paulo state, brazil.	in order to evaluate the potential zoonotic transmission of giardia duodenalis, isolates from humans and dogs in the northwestern region of the são paulo state, brazil were characterized based on the β-giardin gene.	2011	21861011
Occurrence of Ancylostoma in dogs, cats and public places from Andradina city, São Paulo state, Brazil.	The aim of this study was to determine the frequency and intensity of Ancylostoma spp. in 33 dogs and 52 cats by means of coproparasitological examinations and parasitological necropsy, and assess the presence of contaminated feces with eggs of that parasite in public places of Andradina Municipality, São Paulo State, Brazil. Willis-Mollay and Sedimentation methods indicated Ancylostoma spp. eggs in 87.8% (29/33) dogs and 94.2% (49/52) cats. The species A. caninum and A. braziliense were found i ...	2011	21915459
first occurrence of an autochthonous canine case of leishmania (leishmania) infantum chagasi in the municipality of campinas, state of são paulo, brazil.	an autochthonous case of visceral leishmaniasis is reported in a dog (canis familiaris) as an apparently natural infection in a non-endemic area. dna obtained from spleen and liver samples produced the expected fragment in a leishmania-specific rdna-based nested-pcr assay. the pcr product, a 490 bp fragment, was sequenced and the nucleotide sequence was identical to that of leishmania (leishmania) infantum chagasi. these results are surprising since no autochthonous human or canine cases of visc ...	2011	21915468
molecular and serological detection of leishmania spp. in captive wild animals from ilha solteira, sp, brazil.	leishmaniasis is a zoonotic disease that affects 12 million people worldwide. several mammalian species can serve as a reservoir for this disease. dogs are the main reservoir for visceral leishmaniasis in urban areas, which has become a serious public health concern in brazil. the aim of this study was to evaluate the presence of leishmania spp. in captive wild animals from ilha solteira, são paulo, brazil. blood and various tissues samples were collected from animals of five different species: ...	2011	21961752
[Zoonosis transmission risk factors according to population habits inIlha Solteira city, Brazil].	Determining conditions regarding possible zoonosis transmission risk based on Ilha Solteira-São Paulo citizens' habits aimed at establishing concrete recommendations for the corresponding local authorities to reduce some risk factors.	2011	22031002
outbreaks of attacks by hematophagous bats in isolated riverine communities in the brazilian amazon: a challenge to rabies control.	human rabies has re-emerged in latin america due to bat associated transmission. we present data related to attacks by hematophagous bats in three riverine communities in the rio negro basin, brazilian amazon. a cross-sectional survey was carried out to obtain demographic and epidemiological data through interviews with 201 inhabitants. a total of 721 bat attacks with bites took place from 2004 to 2006, 238 (33%) reported by residents in campinas do rio preto, 329 (46%) in águas vivas and 154 (2 ...	2011	22171969
[evaluation of rabies post-exposure prophylaxis in humans injured by dogs and cats in the municipality of jaboticabal, sp, from 2000 through 2006].	the present study aimed to evaluate rabies post-exposure prophylaxis (pep) in humans in the municipality of jaboticabal, são paulo from 2000 through 2006. a descriptive and retrospective study was conducted by collecting data available in patient records. vaccination costs were also calculated; 2,493 patients injured by animals received pep, totaling r$ 179,105.14 and 7,108 doses; 2,184 (71.5%) out of the total reports were caused by dogs and cats clinically healthy at the moment of the attack. ...	2011	22218670
molecular characterization of rabies virus isolated from non-haematophagous bats in brazil.	rabies is an important zoonosis that causes thousands of deaths worldwide each year. although the terrestrial cycle, mainly transmitted by dogs, is controlled in brazil, the aerial cycle remains a serious public health issue, besides the economic problem. in the aerial cycle, the haematophagous bat desmodus rotundus is the main source of infection, where several different species of non-haematophagous bats can be infected and can transmit the virus.	2011	22231241