Publications

termination complex in escherichia coli inhibits sv40 dna replication in vitro by impeding the action of t antigen helicase.	dna replication terminus (ter)-binding protein (tbp) in escherichia coli binds specifically to the terminus (ter) site, and the resulting complex severely blocks dna replication in an unique orientation by inhibiting the action of helicases. to generalize the intrinsic nature of the orientated ter-tbp complex against various helicases, we tested the potential of the complex to inhibit the action of three helicases, dna helicase i, simian virus 40 (sv40) large tumor (t) antigen, and helicase b, d ...	1992	1312088
a mouse cdc25 homolog is differentially and developmentally expressed.	the timing and activation of the p34cdc2 kinase in mammals is associated with dephosphorylation of phosphotyrosine and phosphothreonine residues on the p34cdc2 kinase. for fission yeast, the timing of mitosis is regulated by cyclic accumulation of cdc25, which promotes dephosphorylation of p34cdc2 and concomitant protein kinase activation. we report the identification and characterization of a structural and functional mouse homolog, cdc25m2, of the cdc25 phosphatase. cdc25m2 shows high sequence ...	1992	1313771
in vitro isoprenylation and membrane association of mouse rod photoreceptor cgmp phosphodiesterase alpha and beta subunits expressed in bacteria.	we investigated the specificity of caax box-related isoprenylation of rod photoreceptor cgmp phosphodiesterase (pde) subunits expressed in bacteria and the consequences of this modification on rod disk membrane association. full-length cdna sequences of the alpha and beta subunits of mouse pde, inserted into bacterial pet expression vectors, were overexpressed as fusion proteins containing 28 (bmp-alpha) and 26 (bmp-beta) additional amino acid residues at their n termini. both fusion proteins we ...	1992	1314827
expression and purification of mouse timp-1 from e. coli.	tissue inhibitors of metalloproteinases (timps) constitute a family of secreted glycoproteins involved in regulating extracellular matrix degradation in both normal and malignant tissues. we have expressed a cdna clone of mouse timp-1 as a 22-kda protein with 12 cysteine residues in e. coli and purified protein that shows inhibitory activity against collagenase following renaturation by chemical means. the low specific activity and circular dichroism measurements suggest, however, that the renat ...	1992	1322839
molecular cloning of human macrophage capping protein cdna. a unique member of the gelsolin/villin family expressed primarily in macrophages.	macrophage capping protein (mcp) is a ca(2+)-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. the human cdna for mcp has been cloned and sequenced. the derived amino acid sequence predicts a polypeptide of 38.4 kda. human mcp expressed in escherichia coli using a pet12a vector was functionally identical to the native protein purified from rabbit alveolar macrophages with respect to ca2+ sensitivity and ability to block mon ...	1992	1322908
establishment of a mouse model of cystitis and roles of type 1 fimbriated escherichia coli in its pathogenesis.	the role of type 1 fimbriae in promoting bladder colonization and the course of escherichia coli cystitis were examined with type 1 fimbriated strains of clinically isolated e. coli. in the experiments of mice in vivo, intact bladder epithelium showed natural resistance to the adherence of type 1 fimbriated and non-fimbriated e. coli. however, the exfoliation of bladder superficial cells by trypsinization before the bacterial inoculation promoted the adhesion and colonization of type 1 fimbriate ...	1992	1351243
production of a protein a--lymphotoxin hybrid protein for cancer-targeted therapy.	a hybrid protein between staphylococcal protein a and human lymphotoxin, alt, was produced in escherichia coli by expression of a recombinant plasmid containing the respective genes. igg-binding activity of alt was confirmed by western blotting analysis and by affinity purification with igg-sepharose column chromatography. the purified alt had cytotoxicity on mouse l-929 cells and its specific activity was approximately 3.5-5.0 x 10(6) u mg-1. alt was partially degraded by a protease including i ...	1990	1366561
synthesis of a functional anti-phytochrome single-chain fv protein in transgenic tobacco.	we have expressed a synthetic gene that encodes an antigen-binding single-chain fv protein (scfv) in transgenic tobacco plants. the scfv gene was created by polymerase chain reaction (pcr) amplification of the variable domain coding regions from a mouse monoclonal hybridoma cell line. the monoclonal cell line secretes an igg1 antibody that binds to the plant regulatory photoreceptor protein, phytochrome. the cloned scfv gene was expressed initially in escherichia coli and shown to produce a 28 k ...	1992	1368269
overproduction of biologically-active human nerve growth factor in escherichia coli.	a gene coding for human nerve growth factor (hngf) was constructed for expression under control of the trp promoter in e. coli. the plasmid ptrsngf contained a synthetic hngf gene fused, in frame, to the region encoding the beta-lactamase signal peptide. the plasmid ptrlngf contained the same coding sequence as hngf attached downstream from the n-terminal fragment of the trp l gene. e. coli cells harboring ptrsngf produced an amount of hngf constituting 4% of the total cellular protein, and remo ...	1992	1369095
expression of escherichia coli homoserine kinase in mouse 3t3 cells.	the escherichia coli gene for homoserine kinase (thrb) has been cloned into a simian-virus-40-based eukaryotic expression vector which also includes a neomycin-resistance gene. mouse 3t3 cells transfected with this plasmid were selected for resistance and screened for homoserine kinase activity. it has thus been possible to isolate clones which are capable of accumulating homoserine o-phosphate when supplied with homoserine. in broken-cell preparations the kinetic constants for the production of ...	1992	1371387
purification and characterization of soluble forms of human and rat stem cell factor recombinantly expressed by escherichia coli and by chinese hamster ovary cells.	stem cell factor (scf) is a novel, early-acting hematopoietic factor. it was isolated from the medium of a rat cell line in a soluble, processed form (zsebo et al., 1990, cell 63, 195). the cloned human and rat genes encode the soluble form plus additional c-terminal amino acids including a hydrophobic transmembrane domain (martin et al., 1990, cell 63, 203). we have recombinantly expressed forms of human and rat scf corresponding to the soluble, processed form in escherichia coli and in chinese ...	1992	1374224
molecular and immunological characterization of adp-ribosylarginine hydrolases.	mono-adp-ribosylation is a reversible modification of proteins with nad:arginine adp-ribosyltransferases and adp-ribosylarginine hydrolases catalyzing the forward and reverse reactions, respectively. hydrolase activities were present in a variety of animal species, with the highest specific activities found in rat and mouse brain, spleen, and testis. rat and mouse hydrolases were dithiothreitol- and mg(2+)-dependent, whereas the bovine and guinea pig enzymes were dithiothreitol-independent. a ra ...	1992	1375222
a molecular defect in human protoporphyria.	protoporphyria is generally an autosomal dominant disease that is characterized clinically by photosensitivity and hepatobiliary disease and that is characterized biochemically by elevated protoporphyrin levels. the enzymatic activity of ferrochelatase, which catalyzes the last step in the heme biosynthetic pathway, is deficient in all tissues of patients with protoporphyria. in this study, sequencing of ferrochelatase cdnas from a patient with protoporphyria revealed a single point mutation in ...	1992	1376018
monoclonal antibodies against bacterial glucosamine 6-phosphate synthase: production and use for structural studies.	fifteen mouse x rat hybridoma cell lines producing rat monoclonal antibodies (mabs) directed to escherichia coli glucosamine 6-p synthase (glms) were established and characterized. most of them (13/15) are igg2a while 2 were typed as igg1. their kaff ranged from 1.5 x 10(6) to 9.6 x 10(8) m-1 as determined by beatty et al. (1). the epitopes recognized by these mabs were assigned to one of the two catalytical domains of the enzyme (ct1 and ct2) as demonstrated both by elisa and western-blotting u ...	1992	1376719
directly acting geno- and cytotoxic agents from a wild mushroom dermocybe sanguinea.	the wild mushroom, dermocybe sanguinea, contains several anthraquinone pigments, of which emodin (1,3,8-trihydroxy-6-methylanthraquinone) is quantitatively the most important. in our preliminary tests, dermocybe sanguinea extracts were genotoxic without metabolic activation. the ethanol extract of dermocybe sanguinea was fractionated by flash chromatography, and the emodin contents of the fractions were determined by hplc. their genotoxicities were assayed using a bacterial repair assay and sist ...	1992	1381468
flow cytometric detection of drugs altering the dna methylation pattern.	we have developed a model system for assessing the demethylating potential of external agents. disruption in the dna methylation pattern was evaluated at the translational level of the escherichia coli beta-galactosidase coding gene (lacz). we have constructed a clonal cell line (a4/4 cells) derived from the adenovirus-transformed human embryonic kidney 293 strain. the a4/4 cells contain the e. coli lacz gene under the control of the mouse metallothionein 1 promoter which is down-regulated by a ...	1992	1382840
reactivity of monoclonal antibody e5 with endotoxin. i. binding to lipid a and rough lipopolysaccharides.	the murine igm monoclonal antibody (mab) e5 was produced by a hybridoma derived from spleen cells of a mouse immunized with the j5 rough mutant of escherichia coli o111:b4. in a multicenter randomized placebo-controlled clinical trial, e5 has been shown to reduce significantly the mortality and morbidity of patients with gram-negative sepsis. the characteristics of e5 binding to endotoxin were studied in vitro. we report here the results of binding to an extensive panel of rough lipopolysacchari ...	1992	1394865
analysis of human/mouse interleukin-6 hybrid proteins: both amino and carboxy termini of human interleukin-6 are required for in vitro receptor binding.	the multifunctional cytokine interleukin-6 (il-6) is a single polypeptide chain consisting of 184 amino acids in man and 187 amino acids in mouse. despite the relatively high degree of sequence similarity of these two molecules (about 57%), the biological activity in mouse and human il-6 shows species specificity. starting with this observation, we constructed interspecies hybrids with the goal of defining which segments of the human il-6 molecule are involved in human receptor binding. in this ...	1992	1396966
some properties of purified escherichia coli heat-stable enterotoxin ii.	we examined the biological properties of purified escherichia coli heat-stable enterotoxin ii (stii) using mouse intestinal loop assays and compared these properties with those of heat-stable enterotoxin i (sti) and cholera toxin (ct). the action of stii over time differed from those of sti and ct. stii did not alter cyclic gmp or cyclic amp levels in intestinal mucosal cells. our results supported the idea that the mechanism of action of stii in inducing fluid secretion is different from the me ...	1992	1398961
localization of infection using streptavidin and biotin: an alternative to nonspecific polyclonal immunoglobulin.	since favorable images of infection are obtained with radio-labeled nonspecific igg, streptavidin has been considered as an alternative protein in this investigation. the advantage of streptavidin is that once localized it may be targeted with radiolabeled biotin. studies were conducted in a mouse model with an escherichia coli infection in one thigh. indium-111-labeled streptavidin showed equivalent localization to the infection as that obtained with 111in-labeled polyclonal nonspecific igg, ho ...	1992	1403148
antibacterial effect of bovine milk antibody against escherichia coli in a mouse indigenous infection model.	a skim-milk fraction and a whey-protein concentrate (wpc) fraction were prepared from the cows that had been immunized with e. coli isolated from the mouse intestine. the antibacterial effect of these fractions against e. coli was examined. they contained antibody with a high affinity for e. coli strain 48, a representative strain in the mouse intestine, which is composed of a large amount of igg and smaller amounts of iga and igm. although these fractions showed no bactericidal or bacteriostati ...	1992	1406460
precursor structure, expression, and tissue distribution of human guanylin.	heat-stable enterotoxins (sta) are small, cysteine-rich peptides secreted by escherichia coli that are able to induce diarrhea through the stimulation of an intestine-specific receptor-guanylyl cyclase known as star. a 15-amino acid peptide, guanylin, was recently purified from rat jejunum and proposed to be a potential endogenous activator of this receptor. we describe here the cloning and characterization of human and mouse cdnas encoding precursor proteins of 115 and 116 amino acids, respecti ...	1992	1409606
bacterial single-chain antibody fragments, specific for carcinoembryonic antigen.	we have produced single-chain antibody (scfv) fragments in bacteria specific for carcinoembryonic antigen (cea). polymerase chain reaction (pcr) was used for the cloning and modification of the heavy and light variable regions (vh and vl) of the mouse monoclonal antibody (mab) cb-cea.1. a 14-amino acid linker was used in the synthesis of the scfv gene. the vh and vl regions were amplified from cdna by pcr using 5' end fr1 and 3' end constant region primers, and then sequenced. vh was then amplif ...	1992	1418981
unexpected position-dependent expression of h-2 and beta 2-microglobulin/lacz transgenes.	in order to study sequences involved in the developmentally regulated and tissue-specific expression of the class i major histocompatibility complex (mhc) genes, we have constructed several h-2/lacz transgenic lines in which the 5' regulatory sequences of the h-2kb gene are linked to the escherichia coli beta-galactosidase (lacz) gene. in five h-2/lacz lines, the pattern of lacz expression, detected histochemically varied greatly from line to line. none of the h-2/lacz transgenes were transcribe ...	1992	1418983
biochemical and immunological characterization of the dna binding protein (rbp-j kappa) to mouse j kappa recombination signal sequence.	we have investigated whether j kappa recombination signal sequence (rs) binding protein (rbp-j kappa) has any partial catalytic activities involved in the vdj recombination reaction, such as cleavage, ligation, and bending of dna. murine rbp-j kappa protein purified by j kappa-rs affinity chromatography did not show dna cleavage activities but contained a strong dna ligase activity. to obtain a large amount of purified rbp-j kappa protein, recombinant rbp-j kappa was synthesized in escherichia c ...	1992	1429518
alanine scanning mutagenesis identifies surface amino acids on domain ii of pseudomonas exotoxin required for cytotoxicity, proper folding, and secretion into periplasm.	pseudomonas exotoxin a (pe) is a single polypeptide chain that contains 613 amino acids and is arranged into three major structural domains. domain ia is responsible for cell recognition, domain ii for translocation of pe across the membrane, and domain iii for adp-ribosylation of elongation factor 2. recombinant pe can be produced in escherichia coli and is efficiently secreted into the periplasm when an ompa signal sequence is present. to investigate the role of the amino acids located on the ...	1992	1429683
[significance of phenotypical properties in extra-intestinal e. coli infections].	virulence factors (serogroup, haemolysis, haemagglutination, antigens k1, k5, colicinogenicity) and their association with diseases of 3334 escherichia coli strains isolated from different clinical specimens between 1979-1990 were analysed. strains, that were isolated from cerebrospinal fluid of newborns under one month were characterized by certain serogroups (o7, o18, o45, o78, o83), possession of antigen k1 and production of colicin. on the basis of their ld50 they belonged to the pathogenic ...	1992	1437114
rfp is a dna binding protein associated with the nuclear matrix.	we reported that the rfp gene encodes a protein with putative zinc finger domains and was involved in the activation of the ret proto-oncogene. to further characterize the rfp protein, we developed a polyclonal antibody against the product synthesized from a fragment of the rfp cdna expressed in escherichia coli. western blot analysis showed that rfp was identified as a 58 kda protein in cell lysates from four human and rodent cell lines and from mouse testis. in addition, a unique 68 kda protei ...	1992	1437549
genetic control of resistance to enterotoxigenic escherichia coli in infant mice.	dba/2 and cba infant mice orally challenged with bovine enterotoxigenic escherichia coli (etec) strain b80 presented resistance and susceptibility respectively, as measured by mortality rates 6 days after inoculation. serum antibodies agglutinating etec strain b80 had very low titers in both mouse strains. mendelian analysis of resistance on f1 and on segregating back-crosses showed that resistance is genetic and dominant. dominance may be explained either by a mixed control with an overdominant ...	1992	1453928
site-directed mutation makes rabbit calcyclin dimer.	unlike human, rat and mouse calcyclin, purified rabbit calcyclin did not form a dimer on tricine sds-page under non-reduced conditions. based on the internal peptide sequence of rabbit calcylin, we isolated and sequenced a cdna clone encoding calcyclin. the sequence of this clone (pcalc) is 629 bp long and codes 90 amino acid residues of a protein with a molecular mass of 10,153 da. by northern blot analysis, a major band of 0.9 kbp and a minor band of 2.6 kbp were detected in the lung. the reco ...	1992	1459239
high affinity ligand binding is not essential for granulocyte-macrophage colony-stimulating factor receptor activation.	the high affinity receptor of the cytokine granulocyte-macrophage colony-stimulating factor (gm-csf) is a heterodimer composed of two members of the cytokine receptor superfamily. gm-csf binds to the alpha-subunit (gm-r alpha) with low affinity and to the receptor alpha beta complex (gm-r alpha beta) with high affinity. the gm-csf.gm-r alpha beta complex is responsible for biological activity. interactions of the n-terminal helix of mouse gm-csf with mgm-r alpha beta were examined by introducing ...	1992	1460041
[virulence factors in strains of escherichia coli isolated in urinary tract infections].	in 21 strains of e. coli isolated from patients with cystitis and asymptomatic bacteriuria the authors assessed the group, mannososensitive and mannoresistant agglutination of human, bovine, chick, guinea pig, sheep and pig erythrocytes, the production of haemolysis, colicins, aerobactin, the capacity of strains to induce oedema on mouse paws, the lethal effect in mice, the resistance to tetracycline, streptomycin, chloramphenicol, kanamycin, ampicillin and sulfamethoxidine, the transmission of ...	1992	1464082
targeting sequences of the two major peroxisomal proteins in the methylotrophic yeast hansenula polymorpha.	dihydroxyacetone synthase (das) and methanol oxidase (mox) are the major enzyme constituents of the peroxisomal matrix in the methylotrophic yeast hansenula polymorpha when grown on methanol as a sole carbon source. in order to characterize their topogenic signals the localization of truncated polypeptides and hybrid proteins was analysed in transformed yeast cells by subcellular fractionation and electron microscopy. the c-terminal part of das, when fused to the bacterial beta-lactamase or mous ...	1992	1465101
[adherence of e. coli to bladder epithelia in compromised mice and plasma endotoxin level].	the role of bacterial adherence in association with complicated urinary tract infections (uti) and the correlation between bacterial adherence and plasma endotoxin (et) levels were experimentally investigated by using mouse uti models. mice with foreign bodies induced in the bladder or voiding dysfunction were more susceptible to uti than untreated mice. but diabetic or granulocytopenic mice were little susceptible to uti in comparison with other two models. adherence activities of 6 strains of ...	1992	1474705
molecular analysis of the aroa gene of pasteurella multocida and vaccine potential of a constructed aroa mutant.	the aroa gene from pasteurella multocida was cloned by complementation of the escherichia coli aroa mutant ab2829 with a dna library constructed in puc18. the nucleotide sequence of the p. multocida aroa gene indicated an open reading frame encoding a protein of 441 amino acids, which showed a high degree of homology with the amino acid sequences of various other bacterial aroa proteins. the cloned p. multocida aroa gene was inactivated by insertion of a kanamycin-resistance gene and reintroduce ...	1992	1474900
high affinity dna-microtubule interactions: evidence for a conserved dna-map interaction involving unusual high cscl density repetitious dna families.	we have examined high affinity interactions of chick brain microtubule proteins with 35s labelled tracer dnas from chick, mouse and d. melanogaster under equilibrium conditions by the nitrocellulose filter binding technique. ternary reaction mixtures of the above two components and a third component, an excess of unlabelled competitor dna from either e. coli., mouse, d. melanogaster or chick, were used to measure small fractions of dna in each case (1-4%) bound to microtubule protein under high ...	1992	1488054
effect of serum amyloid p component level on transthyretin-derived amyloid deposition in a transgenic mouse model of familial amyloidotic polyneuropathy.	to elucidate the pathogenesis of amyloid deposition associated with familial amyloidotic polyneuropathy (fap), we developed several transgenic mouse lines carrying the human mutant transthyretin (ttr) gene. we found that human ttr and mouse serum amyloid p component (sap) are deposited as amyloid in tissues of these mouse lines. because sap is a major acute-phase reactant in mice, we asked whether repeated injections of escherichia coli lipopolysaccharide (lps) would enhance the amyloid depositi ...	1992	1497094
effect of ambient temperature and e. coli endotoxin upon the plasma iron level in wild house mice in winter season.	the effects of ambient temperatures of 10 degrees c and 30 degrees c and of e. coli endotoxin on brain temperature and plasma iron level were investigated in unrestrained wild house mice, mus musculus. in control animals (i.p. saline-injected) exposed to cold environment the brain temperature decreased and plasma iron levels were lower than those observed under thermoneutral conditions (30 degrees c). animals injected i.p. with endotoxin (0.5 micrograms.kg-1) and placed at 30 degrees c showed a ...	1992	1506489
crystallization studies of the catalytic subunit of camp-dependent protein kinase: crystals of murine recombinant catalytic subunit and a mutant, cys 343----ser, diffract to 2.7 a resolution.	the recombinant mouse catalytic subunit of camp-dependent protein kinase, expressed and purified from e. coli, has been successfully cocrystallized as a binary complex with an inhibitor peptide and as a ternary complex with an inhibitor peptide and mgatp. in contrast to the catalytic subunit obtained from porcine heart, the recombinant catalytic subunit lacks a myristoyl group at the amino terminus and differs in sequence at nine positions out of 350 amino acids. the catalytic activities of the ...	1992	1515113
response elements of the androgen-regulated c3 gene.	intron and 5'-flanking regions of the androgen-regulated c3 subunit gene contain potential cis-acting transcription control sequences including several 15-base pair (bp) partial palindromes resembling response elements for glucocorticoid (gre) and progesterone (pre) receptors. specific dna binding of the androgen receptor (ar) and androgen-dependent activation of transcription indicate that some of these gre/pre-like sequences are capable of functioning as androgen response elements (are). a 0.3 ...	1992	1537831
a streptavidin-metallothionein chimera that allows specific labeling of biological materials with many different heavy metal ions.	we have designed a streptavidin-metallothionein chimeric protein in which the streptavidin moiety provides a means of binding the metallothionein moiety tightly to specific biological targets. a gene fusion of streptavidin with mouse metallothionein i was efficiently expressed in escherichia coli, and the expressed chimeric protein was purified to homogeneity by a simple procedure. the purified chimera, consisting of four identical subunits, bound one biotin and approximately seven cd2+ ions per ...	1992	1542645
high-fat diets and the immune response of c57bl mice.	as a basis for studies of the influence of lipids on the immune response and health, adult c57bl mice were fed for 10 weeks or longer on one of the following diets: high (200 g/kg) polyunsaturated fatty acid, high (200 g/kg) saturated fatty acid and low (50 g/kg) polyunsaturated fatty acid purified diets and a standard commercial diet. the three test-fat diets were compounded to have approximately the same energy content and the mice of each group maintained similar body-weights. high-fat diets ...	1992	1547199
detailed histopathological examination contributes to the assessment of escherichia coli urovirulence.	to determine the usefulness of detailed histopathological evaluation in the assessment of urovirulence of different escherichia coli strains in a mouse model of ascending, unobstructed urinary tract infection, and to evaluate the relationship between susceptibility to urinary tract infection and renal levels of p fimbrial receptor glycolipids in different mouse strains, female swiss webster and balb/c mice were inoculated transurethrally with one of four different well-characterized wild type e. ...	1992	1552614
high-level temperature-induced synthesis of an antibody vh-domain in escherichia coli using the pelb secretion signal.	we have constructed a temperature-inducible escherichia coli expression vector (ppow) for enhanced secretion of antibody (ab) domains and other foreign proteins. the vector contains the lambda prpl promoters in tandem, and the ci857 gene encoding the temperature-sensitive repressor which provide tight control over protein production. the pelb secretion signal directs the synthesized foreign protein through the cytoplasmic membrane. a mouse ab fragment (the variable heavy (vh) domain of nc41) was ...	1992	1563636
immune response of cattle to haemophilus somnus lipid a-protein conjugate vaccine and efficacy in a mouse abortion model.	immunogenicity of the lipid a component of haemophilus somnus lipooligosaccharide in cattle and mice was examined after purification, detoxification, and covalent conjugation to a protein carrier. after 2 inoculations, a substantial antibody response was induced in most cattle to lipid a and the protein carrier. to determine whether antibodies to lipid a would be protective, 5 x 10(7) colony-forming units of h somnus strain 649 were administered iv to endotoxin-responsive (c3h/hen) mice. in one ...	1992	1575380
engineered disulfide bond greatly increases specific activity of recombinant murine interferon-beta.	unlike other species of interferon-beta (ifn-beta) mouse (mu) ifn-beta has no naturally occurring intramolecular disulfide bond. when expressed in escherichia coli, muifn-beta appears to exhibit instability and low activity. to increase its activity, we engineered a pair of cysteines into recombinant muifn-beta to test whether this change would improve its antiviral activity. in the absence of detailed structural data, the optimal placement of cysteines was determined by sequence comparison with ...	1992	1578187
lps genotype in the c57 black mouse background and its influence on the interleukin-6 response to e. coli urinary tract infection.	the present study examined the influence of the mouse lps genotype on the interleukin-6 (il-6) and polymorphonuclear leucocyte (pmn) responses to mucosal escherichia coli infection. lipopolysaccharide (lps) responder c57bl/6j (lpsn, lpsn) and lps non-responder c57bl/10sccr (lpsd, lpsd) mice were inoculated intravesically with escherichia coli hu734. the secretion of il-6, the recruitment of pmns into urine, and the bacterial clearance from the kidneys and bladders were compared between the two m ...	1992	1579857
plasmodium falciparum: the repetitive msa-1 surface protein of the ro-71 isolate is recognized by mouse antibody against the nonrepetitive repeat block of ro-33.	we have expressed in escherichia coli the nonrepetitive repeat zone of the msa-1 surface protein of the ro-33 isolate of plasmodium falciparum. the recombinant protein was used to immunize mice and the resulting ro-33 monospecific serum was used to screen our p. falciparum strain collection in order to recover additional alleles lacking tripeptide repeats in block 2 of msa-1. only 1 (ro-71) out of 30 isolates tested reacted strongly with the serum by indirect immunofluorescence assay. surprising ...	1992	1592091
gas2, a growth arrest-specific protein, is a component of the microfilament network system.	in this report we analyze the protein product of a growth arrest-specific gene, gas2, by means of an affinity-purified antibody raised against the protein produced in bacteria. the regulation of gas2 biosynthesis reflects the pattern of mrna expression (schneider, c., r. king, and l. philipson. 1988. cell. 54:787-793): its relative level is tightly associated with growth arrest. gas2 seems to be regulated also at the posttranslational level via a phosphorylation mechanism. gas2 is well conserved ...	1992	1607387
enhanced growth restriction of legionella pneumophila in endotoxin-treated macrophages.	macrophages from a/j mice are permissive for growth of legionella pneumophila, an intracellular opportunistic pathogen that grows preferentially in macrophages. macrophages from other mouse strains are highly resistant to growth of legionella. in the present study, it was found that macrophages from a/j mice are readily activated by pretreatment with lipopolysaccharide (lps), so that the cells do not permit legionella to replicate in vitro, as occurs when untreated macrophages from a/j mice are ...	1992	1615009
rat tumor necrosis factor-alpha. transcription in rat kupffer cells and in vitro posttranslational processing based on a pcr-derived cdna.	a dna fragment with a reading frame of 708 basepairs coding for tnf-alpha from rat liver was cloned by the polymerase chain reaction. using this species-specific cdna a biotin-labelled antisense (-)rna was transcribed. this probe was used for northern blot analysis of tnf-alpha gene activation. exposure of rat kupffer cells to lps led to a time-dependent change of tnf-alpha-mrna expression with a maximum between one and two hours after stimulation. in vitro translation was carried out with sense ...	1992	1627266
shigella-type pathomechanism in the "mouse model".	a strain of escherichia coli k-12 carrying the 140-megadalton virulence plasmid of the enteroinvasive e. coli--j53(pspl)--showed high virulence in the "mouse model", in chick embryos, but not in the serény test. it expresses the outer membrane proteins thermoregulatedly, encoded also by the virulence plasmid. in orally infected streptomycin-pretreated mice this strain infects only the large bowel, shows adherence to the epithelial surface, but in its first step preferentially to the mucus excret ...	1992	1632198
mapping of a region of dengue virus type-2 glycoprotein required for binding by a neutralizing monoclonal antibody.	envelope glycoprotein e of flaviviruses is exposed at the surface of the virion, and is responsible for eliciting a neutralizing antibody (ab) response, as well as protective immunity in the host. in this report, we describe a method for the fine mapping of a linear sequence of the e protein of dengue virus type-2 (den-2), recognized by a type-specific and neutralizing monoclonal ab (mab), 3h5. first, an escherichia coli expression vector containing a heat-inducible lambda pl promoter was used t ...	1992	1634111
lipopolysaccharide priming of alveolar macrophages for enhanced synthesis of prostanoids involves induction of a novel prostaglandin h synthase.	we report here that lipopolysaccharide (lps) priming of rabbit alveolar macrophages leads to amplified synthesis of prostanoids, at least in part, by induction of a novel prostaglandin h synthase (pgh synthase). rabbit alveolar macrophages were cultured with or without added lps derived from escherichia coli 0111:b4 for 4 h and then stimulated with opsonized zymosan (opz). lps priming of alveolar macrophages resulted in enhanced release of thromboxane (tx) upon stimulation with opz, when compare ...	1992	1634505
expression, purification, and crystallization of the adipocyte lipid binding protein.	the murine adipocyte lipid binding protein (albp/ap2) has been cloned and expressed in escherichia coli, purified to homogeneity, biochemically characterized, and crystallized for x-ray diffraction study. in the cloning, the albp coding region was placed under control of the reca promoter and downstream of the phage t7 g-10 translation enhancer sequence. nalidixic acid (50 micrograms/ml) induced the expression of albp 20-fold over that attained using the pt7 system previously reported (chinander ...	1991	1650358
altering the conserved nucleotide binding motif in the salmonella typhimurium muts mismatch repair protein affects both its atpase and mismatch binding activities.	the salmonella typhimurium and escherichia coli muts protein is one of several methyl-directed mismatch repair proteins that act together to correct replication errors. muts is homologous to the streptococcus pneumoniae hexa mismatch repair protein and to the duc1 and rep1 proteins of human and mouse. homology between the deduced amino acid sequence of both muts and hexa, and the type a nucleotide binding site consensus sequence, suggested that atp binding and hydrolysis play a role in their mis ...	1991	1651234
nucleoside diphosphate kinase from escherichia coli; its overproduction and sequence comparison with eukaryotic enzymes.	the gene encoding nucleoside diphosphate (ndp) kinase of escherichia coli was identified by polymerase chain reaction using oligodeoxyribonucleotide primers synthesized on the basis of consensus sequences from myxococcus xanthus and various eukaryotic ndp kinases. the gene (ndk), mapped at 54.2 min on the e. coli chromosome, was cloned and sequenced. the e. coli ndp kinase was found to consist of 143 amino acid residues that are 57, 45, 45, 42, 43, and 43% identical to the m. xanthus, dictyostel ...	1991	1657712
interferon-inducible mouse mx1 protein that confers resistance to influenza virus is gtpase.	the murine mx1 protein is an interferon-inducible nuclear protein and confers resistance to influenza virus infection even though the resistance mechanism is yet unclear. the mx1 protein contains a tripartite gtp-binding domain consisting of gxxxxgks, dxxg, and t/nkxd motifs. in the gtpase gene superfamily such as p21ras protein, signal-transducing g protein, and translation elongation factor, the gtpase activity plays a key role in each protein function. here we show that gtpase activity is ind ...	1991	1657964
unusual cell specific expression of a major human cytomegalovirus immediate early gene promoter-lacz hybrid gene in transgenic mouse embryos.	transgenic mice carrying the human cytomegalovirus immediate early gene promoter driving the e. coli lacz gene displayed an unusual cell specific expression of beta-galactosidase during development. lacz expression was first detected in cells lining the apex of the neural fold of day 8.5 embryos. by day 10 of gestation, expression was prominent in the spinal ganglia, the ganglia of cranial nerves v, vii, viii, ix, and x, in a line of cells marking the ventrolateral pathway adjacent to the dermam ...	1991	1659441
expression of escherichia coli f-18 type 1 fimbriae in the streptomycin-treated mouse large intestine.	escherichia coli f-18, isolated from the feces of a healthy human, makes type 1 fimbriae and is an excellent colonizer of the streptomycin-treated mouse large intestine. recently, it was shown that the inability to produce type 1 fimbriae had no effect on the ability of e. coli f-18 to colonize the streptomycin-treated mouse large intestine, suggesting the possibility that e. coli f-18 does not express type 1 fimbriae in vivo. however, we show here that e. coli f-18 does express type 1 fimbriae ...	1991	1672304
expression of mouse tyrosine hydroxylase in escherichia coli.	enzymatically active mouse tyrosine hydroxylase (th) was successfully expressed at a high level in escherichia coli using a t7 rna polymerase directed expression system. the specific activity of mouse th in e. coli cell lysate was 7.5 nmol/mg protein/min. kinetic characteristics of recombinant th were examined. km for tyrosine and (6r)-tetrahydrobiopterin (6r-bh4) cofactor were determined to be 7.2 microm (420 microm 6r-bh4), 19 microm [( 6r-bh4] less than 55 microm, 20 microm tyrosine) and 54 m ...	1991	1677565
direct gene transfer into mouse muscle in vivo.	rna and dna expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and beta-galactosidase were separately injected into mouse skeletal muscle in vivo. protein expression was readily detected in all cases, and no special delivery system was required for these effects. the extent of expression from both the rna and dna constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. in situ cytochemical staining for beta-gal ...	1990	1690918
the fine specificity of anti-la antibodies induced in mice by immunization with recombinant human la autoantigen.	because of increasing evidence suggesting that anti-la autoantibodies are induced in humans by an ag-specific mechanism, we investigated the antibody response of animals immunized with the human la ag and studied its relationship to the anti-la response of autoimmune patients. anti-la antibodies were raised in 6- to 8-wk-old male mrl(-)+/+, c57bl/6j, balb/c, and a/j mice by immunizing with authentic human la protein obtained by recombinant expression in escherichia coli. as we have shown previou ...	1990	1692063
identification and mapping of an immunogenic region of mycoplasma hyopneumoniae p65 surface lipoprotein expressed in escherichia coli from a cloned genomic fragment.	a previously characterized lipid-modified amphiphilic surface protein of mycoplasma hyopneumoniae, p65, has been defined by its reaction with a surface-binding monoclonal antibody (mab) and by its exclusive partitioning into the detergent phase during triton x-114 phase fractionation (k. s. wise and m. f. kim, j. bacteriol. 169:5546-5555, 1987). in the current study, polyclonal mouse antibody (pab) to gel-purified p65 was used to identify recombinant phage plaques expressing p65-related epitopes ...	1990	1695206
synthesis and expression in escherichia coli of dna encoding the murine lambda 1 chain of a monoclonal antibody specific for salmonella serotype b o-antigen.	a 658 bp dna sequence corresponding to the murine lambda 1 chain of a monoclonal antibody, se155-4, specific for the salmonella serotype b o-antigen, was designed using escherichia coli preferred codons and chemically synthesized by ligation of synthetic fragments into a linearized plasmid followed by transformation into e. coli. a synthetic signal peptide (ompa) was fused to express the l chain as a free polypeptide into the periplasm of e. coli cells. after isolation and purification, heterolo ...	1990	1695376
patterns of expression of position-dependent integrated transgenes in mouse embryo.	the abilities to introduce foreign dna into the genome of mice and to visualize gene expression at the single-cell level underlie a method for defining individual elements of a genetic program. we describe the use of an escherichia coli lacz reporter gene fused to the promoter of the gene for hypoxanthine phosphoribosyl transferase that is expressed in all tissues. most transgenic mice (six of seven) obtained with this construct express the lacz gene from the hypoxanthine phosphoribosyltransfera ...	1990	1696727
antigenic conservation of the 15,000-dalton outer membrane lipoprotein pcp of haemophilus influenzae and biologic activity of anti-pcp antisera.	a gene from haemophilus influenzae encoding an outer membrane lipoprotein of about 15,000 daltons and which comigrates with the peptidoglycan-associated lipoprotein (pal) of h. influenzae on sodium dodecyl sulfate-polyacrylamide gel electrophoresis has been previously reported and designated pcp gene, and its product has been designated pcp. in order to obtain specific immunologic probes for the analysis of pcp expression, cellular location, and antigenic conservation in h. influenzae, pcp was f ...	1990	1698180
cloning, expression and characterization of the human transcription elongation factor, tfiis.	the cdna for the human elongation factor, tfiis, has been cloned and expressed in e. coli with the t7 expression system. this 280-amino acid tfiis protein is shorter by 21 residues than that of the mouse. the missing 21 residues are located in the amino-terminal region, which is not thought to be required for transcriptional stimulation. apart from this gap, human and mouse proteins reveal 96% overall identity and 98.5% sequence similarity if conservative substitutions are taken into account. th ...	1991	1708494
production of a recombinant human t-cell leukemia virus type-i trans-activator (tax1) antigen and its utilization for generation of monoclonal antibodies against various epitopes on the tax1 antigen.	a 42-kda recombinant protein, px141, consisting of the trans-activator protein encoded by human t-cell leukemia virus (htlv-1) (tax1 antigen) and the amino-terminal fusion peptide of 12 amino acid residues of the alpha-peptide encoded by the plasmid puc19 was produced. in order to investigate the immunogenicity of the tax1 antigen, mice were immunized with the purified px141 and 4 anti-tax1 monoclonal antibodies (mabs) designated taxy-1, taxy-6, taxy-7 and taxy-8 were generated, and their reacti ...	1991	1710610
synthesis and expression in escherichia coli of cistronic dna encoding an antibody fragment specific for a salmonella serotype b o-antigen.	a 1460-bp dna encoding the two chains of the antigen-binding fragment (fab) portion of a monoclonal antibody have been chemically synthesized and expressed in escherichia coli. the antibody, se155-4, is specific for a salmonella serogroup b o-antigen and its crystal structure is under investigation. the genes were synthesized according to a strategy that allows for easy manipulation in genetic engineering studies of the fab-binding site. each gene is preceded by the ompa secretory signal and a r ...	1991	1711496
aromatic-dependent salmonella as live vaccine presenters of foreign epitopes as inserts in flagellin.	synthetic oligonucleotides specifying amino acid sequences identified as epitopes of various foreign antigens (cholera toxin subunit b, hepatitis b surface protein and others) have been inserted at an ecorv-ecorv deletion site in a cloned salmonella flagellin gene; the resulting plasmids, when placed in flagellin-negative escherichia coli or salmonella sp. strains, caused production of flagellin expressing the epitope. if the chimeric flagellin allowed formation of flagella, the epitope was expo ...	1990	1714093
purification and characterization of the cytokine-induced macrophage nitric oxide synthase: an fad- and fmn-containing flavoprotein.	a soluble nitric oxide (no) synthase activity was purified 426-fold from a mouse macrophage cell line activated with interferon gamma and bacterial lipopolysaccharide by sequential anion-exchange, affinity, and gel filtration chromatography. sds/page of the purified no synthase gave three closely spaced silver-staining protein bands between 125 and 135 kda. when assayed in the presence of l-arginine, nadph, tetrahydrobiopterin, fad, and reduced thiol, purified no synthase had a specific activity ...	1991	1715579
immunoadjuvant activity of oral lactobacillus casei: influence of dose on the secretory immune response and protective capacity in intestinal infections.	lactobacilli, often used as effectors of host functions, could play an important role in maintaining human health by controlling other intestinal microorganisms capable of producing harmful effects. using an experimental model, we studied the effect of different oral doses of lactobacillus casei on the secretory iga response and the protective capacity of the microorganism in preventing intestinal infections. the optimization of the protective dose of lb. casei by previous feeding and the use of ...	1991	1722492
constitutive production of granulocyte colony-stimulating factor by hybrids of a sv40-transformed mouse macrophage and a renal adenocarcinoma cell line.	mouse macrophage bam3 cells produced colony-stimulating factors (csfs) after stimulation with bacterial lipopolysaccharide (lps). by assaying the csf using various interleukin 3-dependent cell lines, it was shown that most of the csfs produced by bam3 cells were granulocyte csf (g-csf). the granulocyte-macrophage csf (gm-csf) gene was also expressed in bam3 cells after stimulation with lps. when bam3 cells were fused with the mouse renal adenocarcinoma cell line rag which does not produce g-csf, ...	1991	1723285
histopathologic-microbiologic correlates of invasiveness in a mouse model of ascending unobstructed urinary tract infection.	to clarify the usefulness of histopathology in evaluating invasiveness during acute cystitis and pyelonephritis in a mouse model of urinary tract infection, findings from bladder and kidney sections of mice inoculated transurethrally with escherichia coli were compared with results of bladder, kidney, spleen, and blood cultures and with changes in peripheral blood leukocyte counts. all of the 14 bladder histopathologic abnormalities evaluated were significantly associated with a positive bladder ...	1992	1730896
a comparative study of specific gene probes and standard bioassays to identify diarrhoeagenic escherichia coli in paediatric patients with diarrhoea in bangladesh.	we compared the usefulness of gene probes with standard bioassays to identify diarrhoeagenic escherichia coli amongst isolates from bangladeshi children under 1 year of age with diarrhoea. e. coli isolates were analysed with specific gene probes for localised adhesiveness (la), diffuse adhesiveness (da), heat-labile toxin (lt), heat-stable toxin (st), shiga-like toxins (slt i and slt ii), and enteroinvasiveness, and in bioassays for production of enterotoxins and cytotoxins, and for cell adheren ...	1992	1731056
cloning and expression of the variable regions of mouse myeloma protein mopc315 in e. coli: recovery of active fv fragments.	recombinant dna techniques were used to clone and express the fv portion of mopc315, a mouse myeloma protein with a high affinity for 2,4-dinitrophenyl (dnp). the fv fragment consists of a heterodimer of heavy and light chain variable domains (vh and vl). two separate bacterial plasmid constructs, containing either a variable region cdna for the light chain or a variable region synthetic gene for the heavy chain demonstrated high levels of expression (150-200 mg/l) under control of the bacteriop ...	1992	1731188
a fully active variant of dihydrofolate reductase with a circularly permuted sequence.	the amino acid sequence of mouse dihydrofolate reductase was permuted circularly at the level of the gene. by transposing the 3'-terminal half of the coding sequence to its 5' terminus, the naturally adjacent amino and carboxyl termini of the native protein were fused, and one of the flexible peptide loops at the protein surface was cleaved. the steady-state kinetic constants, the dissociation constants of folate analogues, and the degree of activation by both mercurials and salt as well as the ...	1992	1737018
development and characterization of a panel of monoclonal antibodies against the novel subtilisin-like proprotein processing enzyme furin.	monoclonal antibodies were raised against the recently discovered subtilisin-like proprotein processing enzyme furin. as immunogen, a bacterially expressed hybrid protein was used which consisted of glutathione s-transferase fused to almost the entire human furin protein. ten monoclonal antibodies were obtained and these could be divided into four categories on the basis of their reactivity towards a number of bacterially expressed hybrid proteins, each of which contained a different portion of ...	1992	1737642
protective effect of tissue ferritins in experimental escherichia coli infection of mice in vivo.	the effect of ferritins from horse (fh) and bovine (fb) spleen and murine liver (fm) on the survival rate of cfw mice lethally infected with escherichia coli (strain 8440-78 k 80/b) was evaluated. ferritins given intravenously 24 h before intravenous inoculation of bacteria, protected mice most effectively from death due to infection. the effect was dose dependent. at 500 micrograms of ferritin per mouse, the maximum survival rates were 86% (fh), 81% (fm) and 79% (fb), while only 5% of the contr ...	1991	1768608
[cloning of the gene and immunochemical specificity of recombinant immunoglobulin binding protein v7 from streptococcus valente (group g)].	the fragment of the structural gene coding for the fc-receptor of streptococcus valente (g group) has been cloned. the resulting recombinant plasmid ppgsv1 contains the o, kb hindiii fragment of streptococcal chromosomal dna inserted into the vector plasmid puc19 and determines the expression of the 31 kd protein in escherichia coli cells. the protein binds the immunoglobulins of human, rabbit, guinea pig origin, but in contrast to the g protein of another g group streptococcus it is nonreactive ...	1991	1784298
[cloning and detailed mapping of the fra-ymt region of the yersinia pestis pfra plasmid].	the genetical libraries of the pfra plasmid of yersinia pestis genes were obtained by insertion into the psti, salgi, ecori, xhoi restriction sites of the cosmid vector phc79. the immunochemical analysis of the recombinant clones has revealed the clones synthesizing the antigen fl (fraction i) and mouse toxin (ymt--yersinia pestis murine toxin). the restriction analysis of the plasmids from antigen synthesizing clones has permitted to construct the detailed physical map of the fra-ymt region of ...	1991	1787840
positive regulator for the expression of mba protein of the virulence plasmid, pkdsc50, of salmonella choleraesuis.	a positive regulator was identified within a 2.3 kb fragment of the 6.4 kb mouse bacteremia region (mba region) of the virulence pkdsc50 plasmid of salmonella choleraesuis. sodium dodecyl sulphate polyacrylamide gel electrophoresis showed that escherichia coli k-12 carrying the recombinant plasmids of the 2.3 kb fragment produced mba1 protein with a molecular mass of 32 kda. the recombinant plasmids carrying a 4.1 kb fragment, the other part of 6.4 kb region, produced mba2 (32 kda), mba3 (70 kda ...	1991	1795622
rapid detection of antigen binding by antibody fragments expressed in the periplasm of escherichia coli.	bacterial expression systems can greatly facilitate protein engineering of antibodies. we have developed a system for high-level expression of antibodies, antibody fragments, or hybrid antibodies with novel effector functions in the periplasm of escherichia coli. from 5 ml of cells, a simple extraction yields sufficient material for sds-gel electrophoresis, detection and characterization of hapten binding. to demonstrate our system, heavy-chain variable regions and lambda 1 light chains of a mou ...	1991	1817258
expression of honeybee prepromelittin as a fusion protein in escherichia coli.	strategies for the expression of precursors of eukaryotic secreted proteins as part of fused proteins in escherichia coli have been explored. a fusion protein with beta-galactosidase at the n-terminal end and honeybee prepromelittin at the c-terminal end (beta-gal-pm) was expressed in low amounts as a cleaved polypeptide, from which the promelittin portion had been removed. inclusion in the induction culture of 10 mm mgcl2 or 8.3% (v/v) ethanol, inhibitors of signal peptidase, gave rise to the f ...	1991	1821810
some requirements for the secretion of tumour necrosis factor and its cytotoxic activity in vitro.	the bacterial lipopolysaccharide (lps) in vitro-induced secretion of tumour necrosis factor (tnf) by mouse macrophages is described. the highest amount of tnf was produced by dba/2 mouse peritoneal macrophages activated by a dose of 1 microgram lps for 3 h. the yield of tnf was higher in the presence of indomethacin during lps induction. a markedly positive effect on tnf yield of the priming of macrophages in vivo was manifested by proliferation of peritoneal macrophages and increased secretion ...	1991	1822445
effect of il-3 and e. coli lps on the proliferation of mouse bone marrow cells in vitro.	bone marrow cells from adult balb/c mice were cultured at 37 degrees c, with 5% co2 in air, in rpmi 1640 medium complemented with fetal calf serum. the addition of il-3 (5% of wehi-3-conditioned medium) or e. coli lipopolysaccharides (lps, 50 micrograms/ml) to the cultures stimulated cell proliferation (1.29- and 1.22-fold, respectively, relative to control culture), whereas the simultaneous addition of the two factors reduced the number of cells recovered by 38% relative to those from control c ...	1991	1823004
an interleukin-1 receptor antagonist blocks lipopolysaccharide-induced colony-stimulating factor production and early endotoxin tolerance.	in this report, administration of a recombinant interleukin-1 receptor antagonist protein to mice was found to inhibit induction of colony-stimulating factor as well as induction of early endotoxin tolerance by lipopolysaccharide. these findings provide direct evidence that interleukin-1 is an intermediate in these two lipopolysaccharide-induced phenomena.	1991	1825485
production and characterization of a monoclonal antibody to the v-mos oncogene protein.	valuable information about proto-oncogenes and their physiological functions has been obtained by studying their expression in normal cells. however, the protein product of the c-mos gene, the cellular homologue of the transforming gene (v-mos) of moloney murine sarcoma virus, has not been detected in normal mouse cells or tissues. here, we have constructed a v-mos expression vector, pri-delta mos, which directs the synthesis of a truncated v-mos gene product, a protein a fusion protein. using t ...	1991	1833309
accumulation of the c-mos protein is correlated with post-natal development of skeletal muscle.	previously we reported that c-mos proto-oncogene rna was developmentally up-regulated during post-natal maturation of the rat skeletal muscle. using two different site-directed affinity-purified antipeptide antibodies we can observe that c-mos product (p43 c-mos) accumulates increasingly during post-natal development of the skeletal muscle and exhibits protein kinase activity. we find that in adult rat p43 c-mos is 10-fold higher in skeletal muscle than in ovaries, and 20- to 40-fold higher than ...	1991	1833718
analysis of n-methyl-n-nitrosourea-induced mutations in a shuttle vector plasmid propagated in mouse o6-methylguanine-dna methyltransferase-deficient cells in comparison with proficient cells.	a shuttle vector plasmid, pyz289, was constructed from the pz189 plasmid and polyoma virus dna. the plasmid contains a supf gene as a marker of mutation and can replicate in both escherichia coli and mouse cells. the pyz289 plasmids treated with n-methyl-n-nitrosourea were passed through mouse cells originating from skin tumors, which are either proficient (hl18) or deficient (hl8) in o6-methylguanine-dna methyltransferase activity, and mutations in the supf gene were analyzed. in the repair-def ...	1991	1834328
differential effects of retinoids on pokeweed mitogen induced b-cell proliferation vs immunoglobulin synthesis.	the effects of three retinoids, all-trans-retinoic acid (ra), 13-cis-retinoic acid (cra), and n-(4-hydroxyphenyl) retinamide (4-hpr) on mouse splenocyte responses to pokeweed mitogen (pwm) and escherichia coli lipopolysaccharide (lps) in vitro were evaluated. all three retinoids caused a dose dependent increase in the proliferative response to pwm. the retinoids hierarchy of efficacy based on potentiation of pwm-induced splenocyte proliferation was ra greater than cra greater than 4-hpr. 13-cis- ...	1991	1837010
bacterial translocation from the gastrointestinal tract in various mouse models for human diabetes.	bacterial translocation from the gastrointestinal (gi) tract to other internal organs was examined in multiple low-dose streptozotocin-injected (m-stz), single large-dose streptozotocin-injected (s-stz), alloxan-injected (alloxan), and non-obese diabetic (nod) mice. the incidence of bacterial translocation from the gi tract to the tested organs among diabetic mice was in the order of m-stz mice greater than s-stz mice greater than nod, alloxan, and control mice. the injections of insulin to m-st ...	1991	1839693
mutations that alter the charge of type i regulatory subunit and modify activation properties of cyclic amp-dependent protein kinase from s49 mouse lymphoma cells.	mutations in regulatory (r) subunit of camp-dependent protein kinase were analyzed from camp-resistant mutants of s49 mouse lymphoma cells by direct sequencing of amplified regions of mutant r subunit cdnas. eight distinct single base-change lesions were identified in 24 independent mutants that were hemizygous for expression of mutant r subunits with altered protein charge. cg----ta transitions predominated, but at----gc transitions and gc----ta transversions were also observed. four of five sp ...	1991	1847378
endogenous incorporation of nitric oxide from l-arginine into n-nitrosomorpholine stimulated by escherichia coli lipopolysaccharide in the rat.	the endogenous formation of nitrate in the rat, mouse and human occurs through cellular processes involving the oxidation of the guanido group of arginine. these processes proceed from arginine to nitric oxide with subsequent conversion to electrophilic nitrosating agents capable of forming carcinogenic nitrosamines. we have now demonstrated that endogenous nitrosamine formation can occur via cells stimulated in vivo by bacterial lipopolysaccharide (lps). the nitrosation of morpholine given to r ...	1991	1849054
investigation of herpes simplex virus type 1 genes encoding multiply inserted membrane proteins.	the herpes simplex virus type 1 genome contains four open reading frames (orfs) which are predicted to encode hydrophobic proteins with the potential to cross a membrane several times. the products of these genes (genes ul10, ul20, ul43 and ul53) have not previously been identified. to investigate the role of these proteins in the virus life cycle, we attempted to inactivate the genes individually by inserting the lacz gene from escherichia coli within the orfs. using this approach we have isola ...	1991	1849972
amino acid misincorporation during high-level expression of mouse epidermal growth factor in escherichia coli.	to determine whether the high-level expression of foreign proteins in escherichia coli can lead to frequent translational errors, we analyzed amino acid misincorporation in mouse epidermal growth factor (megf) produced as a trpe fusion protein. the megf dna does not encode phenylalanine and determining the phenylalanine content of the purified protein will measure missense errors. using this approach, we found an error frequency of about 1 in 40 for codons differing by a single base from those f ...	1991	1852602
novel immunomodulators with pronounced in vivo effects caused by stimulation of cytokine release.	beta-1,3-d-polyglucose derivatives protect mice against otherwise lethal bacterial infections. this protective effect has been considered to be mediated through mononuclear phagocytes. by using radioactive labelling, we localized the beta-1,3-d-polyglucose derivatized microbeads (gdm) during the period following injection. the gdm was recovered mainly in the milky spots of the omentum. in animals treated with gdm, the total white cell number was significantly increased in peritoneal fluid of mic ...	1991	1874801
leukotriene a4 hydrolase: determination of the three zinc-binding ligands by site-directed mutagenesis and zinc analysis.	three mutants of recombinant mouse leukotriene a4 (lta4) hydrolase (3.3.2.6) were produced by site-directed mutagenesis on cdna. the codons corresponding to his-295, his-299, or glu-318 were replaced by codons encoding tyrosine, tyrosine, and glutamine, respectively. the mutated cdnas were expressed in escherichia coli, and the three mutated proteins were purified to apparent homogeneity. none of these mutants contained significant amounts of zinc, as determined by atomic absorption spectrometry ...	1991	1881903
substrate analogues and divalent cations as inhibitors of glutamate decarboxylase from escherichia coli.	to examine the idea that glutamate decarboxylase from e. coli can be a convenient source for the study of the effects of compounds on gaba synthesis in the nervous system, a series of substrate analogues and divalent cations were tested as potential inhibitors of the bacterial enzyme. those analogues exhibiting inhibitor activity did so in a competitive manner. the most effective inhibitors were 3-mercaptopropionic acid, 4-bromoisophthalic acid and isophthalic acid which exhibited ki values of 0 ...	1991	1883399