Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| enzyme-based inverse opals: a facile and promising platform for fabrication of biocatalysts. | a facile and promising approach was developed to fabricate enzyme-based 3d-ordered macroporous biocatalysts (enzyme-based inverse opals) by using the colloidal crystal templating method. horseradish peroxidase- and amylase-based inverse opals were prepared, which verified that this method is suitable for various enzymes. | 2014 | 24722982 |
| highly sensitive microfluidic competitive enzyme immunoassay based on chemiluminescence resonance energy transfer for the detection of neuron-specific enolase. | a microfluidic competitive enzyme immunoassay based on chemiluminescence resonance energy transfer (cret) was developed for highly sensitive detection of neuron-specific enolase (nse). the cret system consisted of horseradish peroxidase (hrp)/luminol as a light donor and fluorescein isothiocyanate as an acceptor. when fluorescein isothiocyanate-labeled antibody binds with hrp-labeled antigen to form immunocomplex, the donor and acceptor are brought close each other and cret occurs in the immunoc ... | 2014 | 24723253 |
| development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin m1 in milk. | a direct competitive chemiluminescent enzyme-linked immunosorbent assay (cl-elisa) for detecting aflatoxin m1 (afm1) was developed. to improve the sensitivity of the assay, a mixture of 3-(10'-phenothiazinyl)-propane-1-sulfonate (sptz) and 4-morpholinopyridine (morph) was used to enhance peroxidase-induced cl. the concentrations of the coating anti-afm1 antibody and the conjugate of afb1 with horseradish peroxidase the conditions of the chemiluminescent assay were varied to optimise the conditio ... | 2014 | 24731347 |
| a rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk. | an immunomagnetic beads-based enzyme-linked immunosorbent assay (imbs-elisa) was developed for the detection of β-casein in bovine milk. immunomagnetic beads (imbs) were employed as the solid phase. the anti-β-casein monoclonal antibody (mcab) bound to imbs was used as capture probe and an anti-β-casein polyclonal antibody (pcab), labelled with horseradish peroxidase (hrp), was employed as detector probe. three reaction and two washing steps were needed. each reaction needed 10 min or less, whic ... | 2014 | 24731368 |
| evaluation of inferior alveolar nerve regeneration by bifocal distraction osteogenesis with retrograde transportation of horseradish peroxidase in dogs. | bifocal distraction osteogenesis has been shown to be a reliable method for reconstructing segmental mandibular defects. however, there are few reports regarding the occurrence of inferior alveolar nerve regeneration during the process of distraction. previously, we reported inferior alveolar nerve regeneration after distraction, and evaluated the regenerated nerve using histological and electrophysiological methods. in the present study, we investigated axons regenerated by bifocal distraction ... | 2014 | 24732938 |
| synergy-based small-molecule screen using a human lung epithelial cell line yields δf508-cftr correctors that augment vx-809 maximal efficacy. | the most prevalent cystic fibrosis transmembrane conductance regulator (cftr) mutation causing cystic fibrosis, δf508, impairs folding of nucleotide binding domain (nbd) 1 and stability of the interface between nbd1 and the membrane-spanning domains. the interfacial stability defect can be partially corrected by the investigational drug vx-809 (3-[6-[[[1-(2,2-difluoro-1,3-benzodioxol-5-yl)cyclopropyl]carbonyl]amino]-3-methyl-2-pyridinyl]-benzoic acid) or the r1070w mutation. second-generation δf ... | 2014 | 24737137 |
| simultaneous electropolymerization and electro-click functionalization for highly versatile surface platforms. | simple preparation methods of chemically versatile and highly functionalizable surfaces remain rare and present a challenging research objective. here, we demonstrate a simultaneous electropolymerization and electro-click functionalization process (seec) for one-pot self-construction of aniline- and naphthalene-based functional polymer films where both polymerization and click functionalization are triggered by applying electrochemical stimuli. cyclic voltammetry (cv) can be applied for the simu ... | 2014 | 24738664 |
| whisker-related circuitry in the trigeminal nucleus principalis: ultrastructure. | trigeminal (v) nucleus principalis (prv) is the requisite brainstem nucleus in the whisker-to-barrel cortex model system that is widely used to reveal mechanisms of map formation and information processing. yet, little is known of the actual prv circuitry. in the ventral "barrelette" portion of the adult mouse prv, relationships between v primary afferent terminals, thalamic-projecting prv neurons, and gamma-aminobutyric acid (gaba)-ergic terminals were analyzed in the electron microscope. prima ... | 2014 | 24738912 |
| macro/nano-gel composite as an injectable and bioactive bulking material for the treatment of urinary incontinence. | many women around the world are suffering from urinary incontinence, defined as the unintentional leakage of urine by external abnormal pressure. although various kinds of materials have been utilized to treat this disease, therapies that are more effective are still needed for the treatment of urinary incontinence. here, we present a macro/nanogel composed of in situ forming gelatin-based macrogels and self-assembled heparin-based nanogels, which can serve as an injectable and bioactive bulking ... | 2014 | 24739122 |
| purification and characterization of novel cationic peroxidases from asparagus acutifolius l. with biotechnological applications. | four novel basic peroxidases, named aap-1, aap-2, aap-3, and aap-4, were purified from asparagus acutifolius l. seeds by cation-exchange and gel filtration chromatographies. the four proteins showed a similar electrophoretic mobility of 46 kda while, by maldi-tof ms, different mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for aap-1, aap-2, aap-3, and aap-4, respectively. n-terminal sequences of aaps 1-4 up to residue 20 showed a high percentage of identity with the peroxida ... | 2014 | 24740695 |
| rapid screening of multiple antibiotic residues in milk using disposable amperometric magnetosensors. | disposable amperometric magnetosensors, involving a mixture of modified-magnetic beads (mbs), for the multiplex screening of cephalosporins (cphs), sulfonamides (sas) and tetracyclines (tcs) antibiotic residues in milk are reported for the first time in this work. the multiplexed detection relies on the use of a mixture of target specific modified magnetic beads (mbs) and application of direct competitive assays using horseradish peroxidase (hrp)-labeled tracers. the amperometric responses measu ... | 2014 | 24745735 |
| [irreversible chemical afm-fishing for the detection of low-copied proteins]. | the atomic-force microscopy-based method of irreversible chemical afm-fishing (afm-if(ch)) has been developed for the detection of proteins at ultra-low concentrations in solution. using this method, a very low concentration of horseradish peroxidase (hrp) protein (10(-17) m) was detected in solution. a theoretical model that allows the description of obtained experimental data, is proposed. this model takes into consideration both the transport of the protein from the bulk solution onto the afm ... | 2014 | 24749246 |
| the brainstem localization of gastric preganglionic parasympathetic neurons in the agouti (dasyprocta leporina). | this study was designed to determine qualitatively, the source of gastric vagal nerve fibres in the agouti. a total of 18 male and female adult agoutis were used for the present investigation. following anaesthesia, laparotomy was performed and the stomach exteriorized. multiple intramuscular injections of wheat germ agglutinin-horseradish peroxidase (wga-hrp) were then made into different areas of the stomach in the experimental animals. the control animals were divided into four groups of two ... | 2013 | 24756734 |
| two-dimensional ordered silicon dioxide cavities array as matrix for the immobilization of horseradish peroxidase and its direct electrochemistry. | a silicon dioxide (sio2) cavities array was fabricated on indium-tin oxide (ito) electrode with polystyrene (ps) particles array as template by using sol-gel technique. the electrochemical properties of sio2 cavities array indicated that it acted as microelectrode array. horseradish peroxidase (hrp) was immobilized on the surface of sio2 cavities by adsorption method. direct electrochemistry of hrp modified sio2 cavities array was studied. the results showed that the microenvironment of sio2 cav ... | 2014 | 24758049 |
| enzymatic preparation of a redox-responsive hydrogel for encapsulating and releasing living cells. | horseradish peroxidase-mediated oxidative cross-linking of a thiolated poly(ethylene glycol) is promoted in the absence of exogenous hydrogen peroxide, by adding a small amount of a phenolic compound under physiological conditions. the prepared hydrogel can encapsulate and release living mammalian cells. | 2014 | 24761434 |
| colorimetric determination of sarcosine in urine samples of prostatic carcinoma by mimic enzyme palladium nanoparticles. | the proposed palladium nanoparticles (pd nps), which with the catalytic activity similar to the horseradish peroxidase (hrp) mimic enzyme, can effectively catalyze the h2o2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine sulfate (tmb) accompanied with a color change from colorless to blue in solution. and as a result, the sensitive detection of sarcosine can be realized by the naked eye observation and ultraviolet spectrophotometry, using pd nps as catalyst and tmb as the substrate of the s ... | 2014 | 24767152 |
| facile fabrication of core cross-linked micelles by raft polymerization and enzyme-mediated reaction. | polymeric micelles formed in aqueous solution by assembly of amphiphilic block copolymers have been extensively investigated due to their great potential as drug carriers. however, the stability of polymeric assembly is still one of the major challenges in delivering drugs to tissues and cells. here, we report a facile route to fabricate core cross-linked (ccl) micelles using an enzymatic polymerization as the cross-linking method. we present synthesis of poly(ethylene glycol)-block-poly(n-isopr ... | 2014 | 24768266 |
| a magnetic particles-based chemiluminescence enzyme immunoassay for rapid detection of ovalbumin. | egg allergy is an important public health and safety concern, so quantification and administration of food or vaccines containing ovalbumin (ova) are urgently needed. this study aimed to establish a rapid and sensitive magnetic particles-chemiluminescence enzyme immunoassay (mps-cleia) for the determination of ova. the proposed method was developed on the basis of a double antibodies sandwich immunoreaction and luminol-h2o2 chemiluminescence system. the mps served as both the solid phase and sep ... | 2014 | 24769049 |
| electrochemical immunosensor for detecting the spore wall protein of nosema bombycis based on the amplification of hemin/g-quadruplex dnazyme concatamers functionalized pt@pd nanowires. | in this work, an ultrasensitive electrochemical immunosensor for detecting the pebrine disease related spore wall protein of nosema bombycis (swp n.b) was fabricated based on the amplification of hemin/g-quadruplex functionalized pt@pd nanowires (pt@pdnws). the synthesized pt@pdnws possessed large surface area, which could effectively improve the immobilization amount of hemin/g-quadruplex dnazyme concatamers produced via hybridization chain reaction (hcr). in the presence of swp n.b, the hemin/ ... | 2014 | 24787126 |
| rapid enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals. | we assessed the utility of an enzyme-linked immunosorbent assay (elisa) for the detection of hantavirus-specific antibodies from sera of oligoryzomys longicaudatus, the principal reservoir of andes virus (andv), using an antigen previously developed for detection of antibodies to sin nombre virus (snv) in sera from peromyscus maniculatus. the assay uses a protein a/g horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. serum samples from oligoryzomys longicaudatus col ... | 2014 | 24806874 |
| procalcitonin sensitive detection based on graphene-gold nanocomposite film sensor platform and single-walled carbon nanohorns/hollow pt chains complex as signal tags. | septicemia is a serious disease that requires early diagnosis, and procalcitonin (pct) serves as a diagnostic biomarker for this disease. traditional clinical detection (via immune-gold chips) remains difficult and expensive. an electrochemical immunosensor based on new nanomaterials may provide a solving approach. herein, an ultrasensitive sandwich electrochemical strategy for pct detection was developed. firstly, reduced graphene oxide (rgo)-gold (au) nano-composite film was used as the immuno ... | 2014 | 24813909 |
| development of an immunosensor for the detection of francisella tularensis antibodies. | tularemia, also known as rabbit fever, is a highly infectious zoonotic disease caused by a non-motile and non-spore-forming gram-negative coccoid rod bacterium, francisella tularensis. it occurs naturally in lagomorphs (rabbits and hares), but many animals have been reported to be susceptible. transmission to humans is mostly caused by inhalation of aerosolised bacteria, handling of infected animals, arthropod stings, and ingestion of contaminated foods and water. at present, pathogenic isolatio ... | 2014 | 24817365 |
| nanocomposite incorporating v2o5 nanowires and gold nanoparticles for mimicking an enzyme cascade reaction and its application in the detection of biomolecules. | artificial enzyme mimics are a current research interest, and many nanomaterials have been found to display enzyme-mimicking activity. however, to the best of our knowledge, there have not hitherto been any reports on the use of pure nanomaterials to construct a system capable of mimicking an enzyme cascade reaction. herein, we describe the construction of a novel nanocomposite consisting of v2o5 nanowires and gold nanoparticles (aunps) through a simple and facile chemical method, in which v2o5 ... | 2014 | 24825488 |
| controlling enzymatic activity and kinetics in swollen mesophases by physical nano-confinement. | bicontinuous lipid cubic mesophases are widely investigated as hosting matrices for functional enzymes to build biosensors and bio-devices due to their unique structural characteristics. however, the enzymatic activity within standard mesophases (in-meso) is severely hindered by the relatively small diameter of the mesophase aqueous channels, which provide only limited space for enzymes, and restrict them into a highly confined environment. we show that the enzymatic activity of a model enzyme, ... | 2014 | 24831024 |
| draft genome sequence of pectobacterium wasabiae strain cfia1002. | pectobacterium wasabiae, originally causing soft rot disease in horseradish in japan, was recently found to cause blackleg-like symptoms on potato in the united states, canada, and europe. a draft genome sequence of a canadian potato isolate of p. wasabiae cfia1002 will enhance the characterization of its pathogenicity and host specificity features. | 2014 | 24831134 |
| a new restriction endonuclease-based method for highly-specific detection of dna targets from methicillin-resistant staphylococcus aureus. | pcr multiplexing has proven to be challenging, and thus has provided limited means for pathogen genotyping. we developed a new approach for analysis of pcr amplicons based on restriction endonuclease digestion. the first stage of the restriction enzyme assay is hybridization of a target dna to immobilized complementary oligonucleotide probes that carry a molecular marker, horseradish peroxidase (hrp). at the second stage, a target-specific restriction enzyme is added, cleaving the target-probe d ... | 2014 | 24831802 |
| nucleic acid-mediated intracellular protein delivery by lipid-like nanoparticles. | intracellular protein delivery has potential biotechnological and therapeutic application, but remains technically challenging. in contrast, a plethora of nucleic acid carriers have been developed, with lipid-based nanoparticles (lnps) among the most clinically advanced reagents for oligonucleotide delivery. here, we validate the hypothesis that oligonucleotides can serve as packaging materials to facilitate protein entrapment within and intracellular delivery by lnps. using two distinct model p ... | 2014 | 24831975 |
| detection of glucose using immobilized bienzyme on cyclic bisureas-gold nanoparticle conjugate. | a highly sensitive electrochemical glucose sensor has been developed by the co-immobilization of glucose oxidase (gox) and horseradish peroxidase (hrp) onto a gold electrode modified with biocompatible cyclic bisureas-gold nanoparticle conjugate (cbu-aunp). a self-assembled monolayer of mercaptopropionic acid (mpa) and cbu-aunp was formed on the gold electrode through a layer-by-layer assembly. this modified electrode was used for immobilization of the enzymes gox and hrp. both the hrp and gox r ... | 2014 | 24835425 |
| graphene quantum dots and the resonance light scattering technique for trace analysis of phenol in different water samples. | a novel, highly selective resonance light scattering (rls) method was researched and developed for the analysis of phenol in different types of industrial water. an important aspect of the method involved the use of graphene quantum dots (gqds), which were initially obtained from the pyrolysis of citric acid dissolved in aqueous solutions. the gqds in the presence of horseradish peroxidase (hrp) and h2o2 were found to react quantitatively with phenol such that the rls spectral band (310 nm) was ... | 2014 | 24840454 |
| fe(iii)-taml activator: a potent peroxidase mimic for chemiluminescent determination of hydrogen peroxide. | efforts to replace native peroxidase with its low molecular weight alternatives have stimulated a search for peroxidase mimetics. herein we describe the oxidation of luminol with hydrogen peroxide catalyzed by commercially available fe(iii)-taml activator 1a, which was shown to be a more active catalyst than hemin. at fe(iii)-taml activator 1a use in chemiluminescent assay for h2o2 determination the detection limit value (3σ) of 5×10(-8)m was similar to the detection limit obtained with horserad ... | 2014 | 24840457 |
| the effect of leonurus cardiaca herb extract and some of its flavonoids on mitochondrial oxidative phosphorylation in the heart. | motherwort (leonurus cardiaca) possesses antibacterial, antioxidant, anti-inflammatory, and analgesic activities, and is used as a complementary remedy to improve heart function and blood circulation. since cardiovascular diseases are often associated with an alteration of mitochondria, the main producers of atp in cardiac muscle cells, the aim of our work was to determine bioactive constituents present in motherwort aerial parts extract in ethanol and investigate their effects on the functions ... | 2014 | 24841965 |
| development of an enzyme-linked-receptor assay based on syrian hamster β2-adrenergic receptor for detection of β-agonists. | β-adrenergic agonists (β-agonists) are illegally used in animal husbandry, threatening the health of consumers. to realize multianalyte detection of β-agonists, a β2-adrenergic receptor (β2-ar) was cloned from syrian hamster lung and heterogeneously expressed by spodoptera frugiperda (sf9) cells. the recombinant β2-ar was purified from intracellular soluble proteins of infected sf9 cells, and was utilized to establish an enzyme-linked-receptor assay (elra) to detect a group of β-agonists simulta ... | 2014 | 24853343 |
| electrochemical monitoring of the early events of hydrogen peroxide production by mitochondria. | mitochondria consume oxygen in the respiratory chain and convert redox energy into atp. as a side process, they produce reactive oxygen species (ros), whose physiological activities are still not understood. however, current analytical methods cannot be used to monitor mitochondrial ros quantitatively and unambiguously. we have developed electrochemical biosensors based on peroxidase-redox polymer-modified electrodes, providing selective detection of h2o2 with nanomolar sensitivity, linear respo ... | 2014 | 24854602 |
| aminophenylboronic-acid-conjugated polyacrylic acid-mn-doped zns quantum dot for highly sensitive discrimination of glycoproteins. | discrimination of glycoproteins with different glycans is a significant but difficult issue. we presented here a new strategy for strengthening the discrimination of glycoproteins by introducing a new signaling channel, fluorescence polarization (fp), into a "single probe with three signaling channels" sensor array. the single probe was aminophenylboronic-acid-conjugated poly(acrylic acid)-mn-doped zns quantum dots, and the three signaling channels were fp, room temperature phosphorescence and l ... | 2014 | 24854708 |
| pegylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays. | in this study, magnetic poly(glycidyl methacrylate) microparticles containing carboxyl groups (pgma-cooh) were coated using highly hydrophilic polymer poly(ethylene glycol) (peg). peg was used to reduce nonspecific interactions with proteins and cells while decreasing adhesion of particles to the walls of a microfluidic devices from poly(dimethylsiloxane) (pdms) and cyclic olefin copolymer (coc). zeta potential measurement, infrared spectroscopy, scanning electron microscopy, anti-peg elisa assa ... | 2014 | 24857498 |
| reporter assay for endo/lysosomal escape of toxin-based therapeutics. | protein-based therapeutics with cytosolic targets are capable of exhibiting their therapeutic effect once they have escaped from the endosomes or lysosomes. in this study, the reporters-horseradish peroxidase (hrp), alexa fluor 488 (alexa) and ricin a-chain (rta)-were investigated for their capacity to monitor the endo/lysosomal escape of the ribosome-inactivating protein, saporin. the conjugates-saporin-hrp, (alexa)saporin and saporin-kq-rta-were constructed, and the endo/lysosomal escape of th ... | 2014 | 24859158 |
| glyco-variant library of the versatile enzyme horseradish peroxidase. | when the glycosylated plant enzyme horseradish peroxidase (hrp) is conjugated to specific antibodies, it presents a powerful tool for medical applications. the isolation and purification of this enzyme from plant is difficult and only gives low yields. however, hrp recombinantly produced in the yeast pichia pastoris experiences hyperglycosylation, which impedes the use of this enzyme in medicine. enzymatic and chemical deglycosylation are cost intensive and cumbersome and hitherto existing p. pa ... | 2014 | 24859724 |
| organophosphate vapor detection on gold electrodes using peptide nanotubes. | peptide nanotubes (pnts) encapsulating horseradish peroxidase and surface coated with acetylcholinesterase (ache) were attached to gold screen printed electrodes to construct a novel gas phase organophosphate (op) biosensor. when the sensor with the ache enzyme is put in contact with acetylthiocholine (atch), the atch is hydrolyzed to produce thiocholine, which is then oxidized by horseradish peroxidase (hrp). direct electron transfer between hrp and electrode is achieved through pnts. the signa ... | 2014 | 24861572 |
| localization of proteins in paint cross-sections by scanning electrochemical microscopy as an alternative immunochemical detection technique. | the qualitative identification of proteinaceous substances, as well as their location within a complex paint stratigraphy, is one of the most challenging issues in the characterization of painting materials. nevertheless, information on paint components represent a crucial task for studies concerning both the ancient painting techniques adopted and the state of conservation, being fundamental investigations for the selection of appropriate conservation actions. the present research was aimed at ... | 2014 | 24861969 |
| a novel enzyme-linked immunosorbent assay for detection of escherichia coli o157:h7 using immunomagnetic and beacon gold nanoparticles. | this paper presents a functional nanoparticle-enhanced enzyme-linked immunosorbent assay (fnp-elisa) for detection of enterohemorrhagic escherichia coli (ehec) o157:h7. immunomagnetic nanoparticles (immps) conjugated with monoclonal anti-o157:h7 antibody were used to capture e. coli o157:h7. beacon gold nanoparticles (b-gnps) coated with polyclonal anti-o157:h7 and biotin single-stranded dna (b-dna) were then subjective to immunoreaction with e. coli o157:h7, which was followed by streptavidin-h ... | 2014 | 24864164 |
| sensitive and specific enzyme-linked immunosorbent assay for detecting serum antibodies against mycobacterium avium subsp. paratuberculosis in fallow deer. | the enzyme-linked immunosorbent assay (elisa) is the diagnostic test most commonly used in efforts to control paratuberculosis in domestic ruminants. however, commercial elisas have not been validated for detecting antibodies against mycobacterium avium subsp. paratuberculosis in wild animals. in this study, we compared the sensitivities and specificities of five elisas using individual serum samples collected from 41 fallow deer with or without histopathological lesions consistent with paratube ... | 2014 | 24872517 |
| enzymatic etching of gold nanorods by horseradish peroxidase and application to blood glucose detection. | gold nanorods (aunrs) have become some of the most used nanostructures for biosensing and imaging applications due to their plasmon-related optical response, which is highly sensitive toward minute changes in the aunr aspect ratio. in this context, h2o2 has been used to trigger the chemical etching of aunrs, thereby inducing a decrease of their aspect ratio. however, special conditions and relatively high concentrations of h2o2 are usually required, preventing the applicability of the system for ... | 2014 | 24874748 |
| receptor-mediated uptake of labeled transferrin by embryonic chicken dorsal root ganglion neurons in culture. | transferrin is a growth-promoting plasma protein which is known to occur within developing neurons. since little information exists on the process by which transferrin is internalized by neurons, we studied this process using dissociated embryonic chicken dorsal root ganglion neurons in culture. cultured dorsal root ganglion neurons were incubated in the presence of 3.75 nm (125)i-transferrin at 37°c, the cultures were extensively washed, the neurons were solubilized in a triton-containing buffe ... | 1985 | 24874753 |
| stat-1 expression is regulated by igfbp-3 in malignant glioma cells and is a strong predictor of poor survival in patients with glioblastoma. | insulin-like growth factor binding proteins (igfbps) have been implicated in the pathogenesis of glioma. in a previous study the authors demonstrated that igfbp-3 is a novel glioblastoma biomarker associated with poor survival. since signal transducer and activator of transcription 1 (stat-1) has been shown to be regulated by igfbp-3 during chondrogenesis and is a prosurvival and radioresistant molecule in different tumors, the aim in the present study was to explore the functional significance ... | 2014 | 24878287 |
| thermo- and sulfate-controllable bioelectrocatalysis of glucose based on horseradish peroxidase and glucose oxidase embedded in poly(n,n-diethylacrylamide) hydrogel films. | dual-responsive poly(n,n-diethylacrylamide) (pdea) hydrogel films with entrapped horseradish peroxidase (hrp) and glucose oxidase (god) were successfully prepared on electrode surface with a simple one-step polymerization procedure under mild conditions, designated as pdea-hrp-god. cyclic voltammetric (cv) response of electroactive probe k3fe(cn)6 at the film electrodes displayed reversible thermo- and sulfate-responsive switching behavior. for example, at 25 °c, the k3fe(cn)6 demonstrated a wel ... | 2014 | 24888410 |
| intramolecular c(sp(3))h amination of arylsulfonyl azides with engineered and artificial myoglobin-based catalysts. | the direct conversion of aliphatic ch bonds into cn bonds provides an attractive approach to the introduction of nitrogen-containing functionalities in organic molecules. following the recent discovery that cytochrome p450 enzymes can catalyze the cyclization of arylsulfonyl azide compounds via an intramolecular c(sp(3))h amination reaction, we have explored here the ch amination reactivity of other hemoproteins. various heme-containing proteins, and in particular myoglobin and horseradish perox ... | 2014 | 24890656 |
| a novel electrochemical aptasensor for highly sensitive detection of thrombin based on the autonomous assembly of hemin/g-quadruplex horseradish peroxidase-mimicking dnazyme nanowires. | in this work, a new signal amplified strategy was constructed based on isothermal exponential amplification reaction (expar) and hybridization chain reaction (hcr) generating the hemin/g-quadruplex horseradish peroxidase-mimicking dnazyme (hrp-mimicking dnazyme) nanowires as signal output component for the sensitive detection of thrombin (tb). we employed expar's ultra-high amplification efficiency to produce a large amount of two hairpin helper dnas within a minutes. and then the resultant two ... | 2014 | 24890694 |
| target-induced aptamer release strategy based on electrochemical detection of staphylococcal enterotoxin b using gnps-zro2-chits film. | a novel electrochemical aptasensor was developed for ultrasensitive detection of staphylococcal enterotoxin b (seb) by combining signal amplification and target-induced aptamer release strategy. a gold electrode was modified with a nanocomposite made of gold nanoparticles reduced in situ, zirconia nanoparticles, and chitosan. the seb aptamer was hybridized by anchoring the capture probe on the modified gold electrode surface through aus binding. in the presence of seb, the capture probe-aptamer ... | 2014 | 24892561 |
| enzyme-polysaccharide interaction: a method for improved stability of horseradish peroxidase. | with the advent of green technology, use of enzymes as biocatalyst has become increasingly popular. however, in doing so, enzymes can lose their structure and catalytic activity under conditions that might be necessary for other components of processes. compared to other strategies, chemical modification is a simple and effective technique for generating stable enzyme. horseradish peroxidase (hrp; ec 1.11.1.7) was chemically modified by conjugating with 10 different polysaccharides. all polysacc ... | 2014 | 24892735 |
| enzyme-antibody dual labeled gold nanoparticles probe for ultrasensitive detection of κ-casein in bovine milk samples. | a dual labeled probe was synthesized by coating gold nanoparticles (aunps) with anti-κ-cn monoclonal antibody (mcab) and horseradish peroxidase (hrp) enzyme on their surface. the mcab was used as detector and hrp was used as label for signal amplification catalytically oxidize the substrate. aunps were used as bridges between the mcab and hrp. based on the probe, an immunoassay was developed for ultrasensitive detection of κ-cn in bovine milk samples. the assay has a linear response range within ... | 2014 | 24892786 |
| oxidation of polycyclic aromatic hydrocarbons by horseradish peroxidase in water containing an organic cosolvent. | polycyclic aromatic hydrocarbons (pahs) are environmental contaminants that are toxic, mutagenic, and carcinogenic. we investigated the horseradish peroxidase (hrp)-catalyzed oxidation of pahs in water containing n,n-dimethylformamide. four pahs (anthracene, phenanthrene, pyrene, and fluoranthene) were investigated using single-pah and mixed-pah systems. the results provide useful information regarding the preferential oxidation of anthracene over other pahs regardless of the reaction time, enzy ... | 2014 | 24894750 |
| immobilization of horseradish peroxidase enzyme on nanoporous titanium dioxide electrodes and its structural and electrochemical characterizations. | hierarchically ordered, honeycomb-like nanoporous tio2 electrodes are prepared by a simple electrochemical anodization process using ammonium fluoride dissolved in ethylene glycol as an electrolytic medium. formation of hexagonally arranged nanopores along with the tubular structure and anatase crystalline phase of tio2 is confirmed by field emission scanning electron microscope (fesem) and x-ray diffraction (xrd) studies. further, these nanoporous tio2 electrodes are employed as a substrate for ... | 2014 | 24903963 |
| signal enhancement strategy for a micro-arrayed polydiacetylene (pda) immunosensor using enzyme-catalyzed precipitation. | this paper describes a signal enhancement strategy to improve the sensitivity of an antibody-based immunosensor that uses polydiacetylene (pda) liposomes to detect a target protein (human immunoglobulin e [hige]). to achieve ultrasensitive detection, multiple stimuli applied to pda immunosensor chips offer a signal enhancement method that combines the primary immune reaction between antigen and antibody with the sandwich method of polyclonal antibody (pab)-conjugated horseradish peroxidase (hrp) ... | 2014 | 24907539 |
| zinc oxide nanoparticles based microfluidic immunosensor applied in congenital hypothyroidism screening. | in this article, we present an innovative approach for congenital hypothyroidism (cht) screening. this pathology is the most common preventable cause of mental retardation, affecting newborns around the world. its consequences could be avoided with an early diagnosis through the thyrotropin (tsh) level measurement. to accomplish the determination of tsh, synthesized zinc oxide (zno) nanobeads (nbs) covered by chitosan (ch), zno-ch nbs, were covalently attached to the central channel of the desig ... | 2014 | 24908405 |
| gold nanoparticles conjugates-amplified aptamer immunosensing screen-printed carbon electrode strips for thrombin detection. | thrombin plays the role in cardiovascular diseases and regulates many processes in inflammation and could be a feature of many pathological conditions, including the thromboembolic disease, cancer and neurodegenerative diseases. an ultrasensitive and amplified electrochemical sandwich assay using screen-printed carbon electrode (spce) strips for thrombin detection was established in this study. the conductivity and sensing performance of the carbon electrodes were enhanced by using gold nanopart ... | 2014 | 24912033 |
| isolation of the lateral border recycling compartment using a diaminobenzidine-induced density shift. | the migration of leukocytes across the endothelium and into tissue is critical to mounting an inflammatory response. the lateral border recycling compartment (lbrc), a complex vesicular-tubule invagination of the plasma membrane found at endothelial cell borders, plays an important role in this process. although a few proteins have been shown to be present in the lbrc, no unique marker is known. here, we detail methods that can be used to characterize a subcellular compartment that lacks an iden ... | 2014 | 24915828 |
| horseradish peroxidase-catalysed in situ-forming hydrogels for tissue-engineering applications. | in situ-forming hydrogels are an attractive class of implantable biomaterials that are used for biomedical applications. these injectable hydrogels are versatile and provide a convenient platform for delivering cells and drugs via minimally invasive surgery. although several crosslinking methods for preparing in situ forming hydrogels have been developed over the past two decades, most hydrogels are not sufficiently versatile for use in a wide variety of tissue-engineering applications. in recen ... | 2015 | 24916126 |
| stability properties of an ancient plant peroxidase. | plant (class iii) peroxidases have numerous applications throughout biotechnology but their thermal and oxidative stabilities may limit their usefulness. horseradish peroxidase isoenzyme c (hrpc) has good catalytic turnover and is moderately resistant to heat and to excess (oxidizing) concentrations of hydrogen peroxide. in contrast, hrp isoenzyme a2 (hrp a2) has better oxidative but poorer thermal stability, while soybean peroxidase (sbp) displays enhanced thermal stability. intrigued by these ... | 2014 | 24919139 |
| boronate affinity monolith with a gold nanoparticle-modified hydrophilic polymer as a matrix for the highly specific capture of glycoproteins. | as low abundance is the great obstacle for glycoprotein analysis, the development of materials with high efficiency and selectivity for glycoprotein enrichment is a prerequisite in glycoproteome research. herein, we report a new kind of hydrophilic boronate affinity monolith by attaching 4-mercaptophenylboronic acid (mpba) with 2-mercaptoethylamine (mpa) on the gold nanoparticle-modified poly(glycidyl methacrylate-co-poly(ethylene glycol) diacrylate)) monolith for glycoprotein enrichment. with p ... | 2014 | 24920310 |
| amplified voltammetric detection of glycoproteins using 4-mercaptophenylboronic acid/biotin-modified multifunctional gold nanoparticles as labels. | ultrasensitive detection of protein biomarkers is essential for early diagnosis and therapy of many diseases. glycoproteins, differing from other types of proteins, contain carbohydrate moieties in the oligosaccharide chains. boronic acid can form boronate ester covalent bonds with diol-containing species. herein, we present a sensitive and cost-effective electrochemical method for glycoprotein detection using 4-mercaptophenylboronic acid (mba)/biotin-modified gold nanoparticles (aunps) (mba-bio ... | 2014 | 24920899 |
| a high-throughput, precipitating colorimetric sandwich elisa microarray for shiga toxins. | shiga toxins 1 and 2 (stx1 and stx2) from shiga toxin-producing e. coli (stec) bacteria were simultaneously detected with a newly developed, high-throughput antibody microarray platform. the proteinaceous toxins were immobilized and sandwiched between biorecognition elements (monoclonal antibodies) and pooled horseradish peroxidase (hrp)-conjugated monoclonal antibodies. following the reaction of hrp with the precipitating chromogenic substrate (metal enhanced 3,3-diaminobenzidine tetrahydrochlo ... | 2014 | 24921195 |
| development of an electrochemical sensor based on the catalysis of ferrocene actuated hemin/g-quadruplex enzyme for the detection of potassium ions. | sensitive and selective sensors need to be explored to detect the physiological potassium level due to its important role in the living organisms. in the present system, a novel electron transfer mediator actuated electrocatalytical biosensor was demonstrated to assay k(+) based on the conformational change of dna. with the hybridization between the complementary bases and the self-folding of guanine-rich nucleic acid sequence, the horseradish peroxidase-mimicking enzyme (hrp-dnazyme) was formed ... | 2014 | 24921965 |
| covalent immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase & horseradish peroxidase onto plasticized polyvinyl chloride (pvc) strip & its application in serum triglyceride determination. | reusable biostrip consisting enzymes immobilized onto alkylamine glass beads affixed on plasticized pvc strip for determination of triglyceride (tg) suffers from high cost of beads and their detachments during washings for reuse, leading to loss of activity. the purpose of this study was to develop a cheaper and stable biostrip for investigation of tg levels in serum. | 2014 | 24927348 |
| off-line hyphenation of boronate affinity monolith-based extraction with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for efficient analysis of glycoproteins/glycopeptides. | boronate affinity materials have attracted increasing attentions as sample enrichment platforms for glycoproteomic analysis in recent years. however, most of the boronate affinity materials that have already employed for proteomic analysis are suffering from apparent disadvantages, such as alkaline ph for binding, weak affinity, and relatively poor selectivity. benzoboroxoles are a unique class of boronic acids which have showed excellent binding properties for the recognition of cis-diol-contai ... | 2014 | 24928239 |
| heterologous production of horseradish peroxidase c1a by the basidiomycete yeast cryptococcus sp. s-2 using codon and signal optimizations. | in the present study, we attempted to improve the production of recombinant horseradish peroxidase c1a (hrp-c1a; a heme-binding protein) by cryptococcus sp. s-2. both native and codon-optimized hrp-c1a genes were expressed under the control of a high-level expression promoter. when the hrp-c1a gene with native codons was expressed, poly(a) tails tended to be added within the coding region, producing truncated messenger rnas (mrnas) that lacked the 3' ends. codon optimization prevented polyadenyl ... | 2014 | 24928655 |
| cytoarchitecture of the olfactory bulb in the laggard mutant mouse. | the laggard (lag) mutant mouse, characterized by hypomyelination and cerebellar ataxia, is a spontaneously occurring mutant mouse caused by mutation in the kif14 gene. in this mutant mouse, the laminated structures such as the cerebral and cerebellar cortices and the dentate gyrus are cytoarchitecturally abnormal. macroscopically, the olfactory bulb of the lag mutant mouse is smaller in size and more transparent than the normal counterpart. hematoxylin-eosin staining reveals that the mutant olfa ... | 2014 | 24931760 |
| facile synthesis of enzyme-inorganic hybrid nanoflowers and its application as a colorimetric platform for visual detection of hydrogen peroxide and phenol. | this study reports a facile approach for the synthesis of horseradish peroxidise (hrp)-inorganic hybrid nanoflowers by self-assembly of hrp and copper phosphate (cu3(po4)2·3h2o) in aqueous solution. several reaction parameters that affect the formation of the hybrid nanoflowers were investigated and a hierarchical flowerlike spherical structure with hundreds of nanopetals was obtained under the optimum synthetic conditions. the enzymatic activity of hrp embedded in hybrid naonflowers was evaluat ... | 2014 | 24937087 |
| a colorimetric sandwich-type assay for sensitive thrombin detection based on enzyme-linked aptamer assay. | a colorimetric sandwich-type assay based on enzyme-linked aptamer assay has been developed for the fast and sensitive detection of as low as 25 fm of thrombin with high linearity. aptamer-immobilized glass was used to capture the target analyte, whereas a second aptamer, functionalized with horseradish peroxidase (hrp), was employed for the conventional 3,5,3',5'-tetramethylbenzidine (tmb)-based colorimetric detection. without the troublesome antibody requirement of the conventional enzyme-linke ... | 2014 | 24937288 |
| ultra-sensitive biosensor for k-ras gene detection using enzyme capped gold nanoparticles conjugates for signal amplification. | in this study, an ultra-sensitive hairpin dna-based electrochemical dna biosensor for k-ras gene detection is described. gold nanoparticles (au-nps) and horseradish peroxidase (hrp)-streptavidin capped au-nps (has) conjugates are used for signal amplification. initially, hairpin dna dually labeled with thiol at its 5' end and with biotin at its 3' end is immobilized on the surface of au-nps modified electrode, and the hairpin dna is in a "closed" state; hence, the has conjugates are shielded fro ... | 2014 | 24939462 |
| evidence for peroxidase activity in caralluma umbellata. | vast applications of peroxidases create an increasing demand to characterize peroxidases from new sources with more applicability potential. the aim of the present study was to check the presence of peroxidase activity from caralluma umbellata. this is the first report on the c. umbellata peroxidase (cup). the presence of peroxidase was revealed by the histochemical analysis of the stem sections, zymographic studies, and in vitro peroxidase activity assay using various reducing substrates viz., ... | 2014 | 24943097 |
| continuous colorimetric screening assay for detection of d-amino acid aminotransferase mutants displaying altered substrate specificity. | d-amino acid aminotransferase (daat) catalyzes the synthesis of numerous d-amino acids, making it an attractive biocatalyst for the production of enantiopure d-amino acids. to bolster its biocatalytic applicability, improved variants displaying increased activity toward non-native substrates are desired. here, we report the development of a high-throughput, colorimetric, continuous coupled enzyme assay for the screening of daat mutant libraries that is based on the use of d-amino acid oxidase (d ... | 2014 | 24949900 |
| colorimetric detection of copper(ii) ion using click chemistry and hemin/g-quadruplex horseradish peroxidase-mimicking dnazyme. | g-quadruplex-forming sequence can be formed through a copper(i) ion (cu(+))-catalyzed click chemistry between azide- and alkyne-modified short g-rich sequences in aqueous solution, eliminating immobilization and washing steps of conventional assays. the source for cu(+) was generated from the reduction of cu(2+) with the reductant of sodium ascorbate. in the presence of hemin and k(+), the self-assembly of hemin/g-quadruplex structure has the activity of horseradish peroxidase (hrp), which can c ... | 2014 | 24950121 |
| enhancement of enzymatic colorimetric response by silver island films on high throughput screening microplates. | in this study, we report the use of an enzyme-based hybrid platform, which is comprised of silver island films, enzymes (hrp and ap) and high-throughput screening (hts) microplates, to enhance the colorimetric response of enzymatic reactions. the hybrid platform was designed in a two-step process: (i) deposition of sifs onto hts microplates with low, medium, and high loading (refers to the extent of the surface plasmon resonance peak of sifs at 460 nm) using tollen's reaction scheme; and (ii) at ... | 2014 | 24950456 |
| lipoprotein(a) determination in human serum using a nitrilotriacetic acid derivative immunosensing scaffold on disposable electrodes. | a novel strategy for the construction of a disposable integrated amperometric immunosensor for the sensitive and rapid determination of lipoprotein(a) (lp(a)), an important predictor of cardiovascular disease risk, in human serum is reported. the approach uses a sandwich format involving the covalent immobilization of selective capture antibodies (antilp(a)) on the surface of n-[nα,nα-bis(carboxymethyl)-lysine]-12-mercaptododecanamide (hs-nta)-modified screen-printed carbon electrodes (spces). a ... | 2014 | 24952905 |
| membrane inlet mass spectrometry reveals that ceriporiopsis subvermispora bicupin oxalate oxidase is inhibited by nitric oxide. | membrane inlet mass spectrometry (mims) uses a semipermeable membrane as an inlet to a mass spectrometer for the measurement of the concentration of small uncharged molecules in solution. we report the use of mims to characterize the catalytic properties of oxalate oxidase (e.c. 1.2.3.4) from ceriporiopsis subvermispora (csoxox). oxalate oxidase is a manganese dependent enzyme that catalyzes the oxygen-dependent oxidation of oxalate to carbon dioxide in a reaction that is coupled with the format ... | 2014 | 24953692 |
| covalent functionalization of gold nanoparticles as electronic bridges and signal amplifiers towards an electrochemical immunosensor for botulinum neurotoxin type a. | this work introduced an efficient approach for modification of aunps with multicomponents by diazonium salt couplings. the multifunctionalized aunps with protruding functional groups that allow simple bioconjugation to large amounts of biomolecules have been successfully used as electronic bridges and signal amplifiers for an electrochemical immunosensor towards the detection of bont/a. the one-step anchoring aunps strategy has greatly increased the efficiency for attachment of biomolecules and ... | 2014 | 24953841 |
| electrochemical detection of leukemia oncogenes using enzyme-loaded carbon nanotube labels. | we describe an ultrasensitive electrochemical nucleic acid assay amplified by carbon nanotubes (cnts)-based labels for the detection of human acute lymphocytic leukemia (all)-related p185 bcr-abl fusion transcript. the carboxylated cnts were functionalized with horseradish peroxidase (hrp) molecules and target-specific detection probes (dp) via diimide-activated amidation and used to label and amplify the target hybridization signal. the activity of captured hrp was monitored by square-wave volt ... | 2014 | 24961450 |
| [neuropathic pain enhances expression of hcn2 channel in rat cerebrospinal fluid-contacting nucleus]. | the purpose of this research is to explore the distribution and expression of hyperpolarization-activated cyclic nucleotide-gated channels subtype 2 (hcn2) in cerebrospinal fluid (csf)-contacting nucleus in neuropathic pain, and provide experimental evidence to reveal the biological function and regulation mechanisms of csf-contacting nucleus in neuropathic pain. neuropathic pain model was produced by chronic constriction injury (cci) in sprague-dawley (sd) rats. intracerebroventricular injectio ... | 2014 | 24964850 |
| multiwell cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection: development, characterization and comparison with cooled-ccd luminograph. | we propose a disposable multiwell microcartridge with integrated amorphous silicon photosensors array for bio- and chemiluminescence-based bioassays, where the enzymatic reactions and the detection unit are coupled on the same glass substrate. each well, made in a polydimethylsiloxane (pdms) unit, hosts an enzymatic reaction that is monitored by one photosensor of the array. photosensors were characterized in terms of their dark current background noise and response to different wavelengths of v ... | 2014 | 24965161 |
| confined multiple enzymatic (cascade) reactions within poly(dopamine)-based capsosomes. | the design of compartmentalized carriers as artificial cells is envisioned to be an efficient tool with potential applications in the biomedical field. the advent of this area has witnessed the assembly of functional, bioinspired systems attempting to tackle challenges in cell mimicry by encapsulating multiple compartments and performing controlled encapsulated enzymatic catalysis. although capsosomes, which consist of liposomes embedded within a polymeric carrier capsule, are among the most adv ... | 2014 | 24968314 |
| an electrochemical elisa-like immunosensor for mirnas detection based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes. | we design an electrochemical immunosensor for mirna detection, based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes. an original immunological approach is followed, using antibodies directed to dna.rna hybrids. an electrochemical elisa-like amplification strategy was set up using a secondary antibody conjugated to horseradish peroxidase (hrp). hydroquinone is oxidized into benzoquinone by the hrp/h2o2 catalytic system. in turn, benzoquinone is electro ... | 2014 | 24973539 |
| orthogonal enzyme arrays on a dna origami scaffold bearing size-tunable wells. | a new waffle-like dna origami assembly (dna waffle) with nine nanometer-scale wells in a 3 × 3 matrix pattern has been successfully constructed and used as a scaffold for selective nano-patterning of individual protein molecules. the folding pattern of the scaffold was specially designed so that the dimensions of each well could be independently tuned according to the dimensions of the guest nanoparticles. we demonstrated that two distinct proteins, streptavidin (sa) tetramer (d = 5 nm) and anti ... | 2014 | 24974892 |
| a newly developed uroplakin ii antibody with increased sensitivity in urothelial carcinoma of the bladder. | uroplakin ii is a 15-kda protein component of the urothelial plaques that enhance the permeability barrier and strength of the urothelium. studies have shown uroplakin ii messenger rna to be expressed in bladder cancer tissues and peripheral blood of patients with urothelial carcinoma. little is known about the protein expression of uroplakin ii in urothelial carcinoma, possibly because of the absence of a commercially available uroplakin ii antibody. pathologists have used the uroplakin iii ant ... | 2014 | 24978921 |
| highly selective fluorogenic multianalyte biosensors constructed via enzyme-catalyzed coupling and aggregation-induced emission. | the development of a highly selective and fast responsive fluorogenic biosensor for diverse analytes ranging from bioactive small molecules to specific antigens is highly desirable but remains a considerable challenge. we herein propose a new approach by integrating substrate-selective enzymatic reactions with fluorogens exhibiting aggregation-induced emission feature. tyrosine-functionalized tetraphenylethene, tpe-tyr, molecularly dissolves in aqueous media with negligible fluorescence emission ... | 2014 | 24983204 |
| silica nanoparticle-based microfluidic immunosensor with laser-induced fluorescence detection for the quantification of immunoreactive trypsin. | the purpose of this study was to develop a silica nanoparticle-based immunosensor with laser-induced fluorescence (lif) as a detection system. the proposed device was applied to quantify the immunoreactive trypsin (irt) in cystic fibrosis (cf) newborn screening. a new ultrasonic procedure was used to extract the irt from blood spot samples collected on filter papers. after extraction, the irt reacted immunologically with anti-irt monoclonal antibodies immobilized on a microfluidic glass chip mod ... | 2014 | 24983904 |
| horseradish peroxidase enzyme immobilized graphene quantum dots as electrochemical biosensors. | green colour emitting graphene quantum dots (gqds) are prepared by a simple acid reflux reaction of graphene oxide (go) produced using a modified hummer's method. structural and morphological characterizations of such gqds are performed using spectroscopic (ftir, uv-vis and photoluminescence) and microscopic (transmission electron microscopy) techniques. these studies reveal the formation of stable, uniform spherical particles of gqds which emit a green colour and possess surface functional moie ... | 2014 | 24984603 |
| apoptotic potential role of agave palmeri and tulbaghia violacea extracts in cervical cancer cells. | cervical cancer, a gynaecological malignant disorder, is a common cause of death in females in sub-saharan africa, striking nearly half a million of lives each year worldwide. currently, more than 50 % of all modern drugs in clinical use are of natural products, many of which have an ability to control cancer cells (madhuri and pandey, curr sci 96:779-783, 2009; richter, traditional medicines and traditional healers in south africa, 2003). in south africa, plants used to treat cancer are rare ev ... | 2014 | 24993114 |
| impedance-derived electrochemical capacitance spectroscopy for the evaluation of lectin-glycoprotein binding affinity. | characterization of lectin-carbohydrate binding using label-free methods such as impedance-derived electrochemical capacitance spectroscopy (ecs) is desirable to evaluate specific interactions, for example, artinm lectin and horseradish peroxidase (hrp) glycoprotein, used here as a model for protein-carbohydrate binding affinity. an electroactive molecular film comprising alkyl ferrocene as a redox probe and artinm as a carbohydrate receptive center to target hrp was successfully used to determi ... | 2014 | 24994505 |
| oxygen-mediated enzymatic polymerization of thiol-ene hydrogels. | materials that solidify in response to an initiation stimulus are currently utilized in several biomedical and surgical applications; however, their clinical adoption would be more widespread with improved physical properties and biocompatibility. one chemistry that is particularly promising is based on the thiol-ene addition reaction, a radical-mediated step-growth polymerization that is resistant to oxygen inhibition and thus is an excellent candidate for materials that polymerize upon exposur ... | 2014 | 24995128 |
| carbon nanohorns as a scaffold for the construction of disposable electrochemical immunosensing platforms. application to the determination of fibrinogen in human plasma and urine. | we describe in this work a novel electrochemical immunosensor design making use of carbon nanohorns (cnhs) as a scaffold for the preparation of disposable immunosensing platforms for the determination of fibrinogen (fib). the approach involved the immobilization of fib onto activated cnhs deposited on screen-printed carbon electrodes (spces) and the implementation of an indirect competitive assay using anti-fib labeled with horseradish peroxidase (hrp) and hydroquinone (hq) as the redox mediator ... | 2014 | 25001594 |
| electrocatalytic activity of core/shell magnetic nanocomposite. | electrically active magnetic nanocomposites (eamncs), au nanoparticles/self-doped polyaniline@fe3o4 (aunps/span@fe3o4) with well-defined core/shell structure, were first synthesized by a simple method. the morphology and composition of the as-synthesized aunps/span@fe3o4 nanocomposite have been characterized by transmission electron microscopy (tem), scanning electron microscopy (sem), fourier transform infrared (ft-ir), ultraviolet-visible (uv-vis), x-ray powder diffraction (xrd), and thermogra ... | 2014 | 25009106 |
| integrating biochemiluminescence detection on smartphones: mobile chemistry platform for point-of-need analysis. | in this paper, we report, for the first time, the use of a smartphone to image and quantify biochemiluminescence coupled biospecific enzymatic reactions to detect analytes in biological fluids. using low-cost three-dimensional (3d) printing technology, we fabricated a smartphone accessory and a minicartridge for hosting biospecific reactions. as a proof-of-principle, we report two assays: a bioluminescence assay for total bile acids using 3α-hydroxyl steroid dehydrogenase coimmobilized with bact ... | 2014 | 25017302 |
| whisker-related circuitry in the trigeminal nucleus principalis: topographic precision. | single whiskers are topographically represented in the trigeminal (v) nucleus principalis (prv) by a set of cylindrical aggregates of primary afferent terminals and somata (barrelettes). this isomorphic pattern is transmitted to the thalamus and barrel cortex. however, it is not known if terminals in prv from neighboring whiskers interdigitate so as to violate rules of spatial parcellation predicted by barrelette borders; nor is it known the extent to which higher order inputs are topographic. t ... | 2015 | 25019347 |
| single-molecule enzymatic conformational dynamics: spilling out the product molecules. | product releasing is an essential step of an enzymatic reaction, and a mechanistic understanding primarily depends on the active-site conformational changes and molecular interactions that are involved in this step of the enzymatic reaction. here we report our work on the enzymatic product releasing dynamics and mechanism of an enzyme, horseradish peroxidase (hrp), using combined single-molecule time-resolved fluorescence intensity, anisotropy, and lifetime measurements. our results have shown a ... | 2014 | 25025461 |
| femtogram detection of horseradish peroxidase by a common desktop scanner. | we report an image-based detection of horseradish peroxidase (hrp) by different color spaces. the results show excellent correlation between color saturation and absorbance (pearson correlation coefficient; 0.9868) with respect to hrp. the present method can detect 185 and 46.45 fg/ml of hrp using o-phenylenediamine dihydrochloride and 3,3',5,5'-tetramethylbenzidine as chromogenic substrates respectively. | 2015 | 25027707 |
| synthesis of hierarchical iron hydrogen phosphate crystal as a robust peroxidase mimic for stable h₂o₂ detection. | to develop a green, cost-efficient and robust peroxidase mimic, micro/nano hierarchical morphology (for ease of separation and reuse), relative chemically stable composition (for ease of storage) and stable crystal structure (for long-term stability) are highly desired. herein, using phosphoric acid as a chelating ligand to control the release of iron ions, hierarchical iron(iii) hydrogen phosphate hydrate crystals are successfully prepared by nanosheets formation and following self-assembling i ... | 2014 | 25029358 |
| biogenic magnetic nanoparticles from burkholderia sp. yn01 exhibiting intrinsic peroxidase-like activity and their applications. | a novel bacterial strain containing biogenic magnetic nanoparticles (bmnps) was isolated from the sediments of songhua river in harbin, china, and was identified as burkholderia sp. yn01. extracted bmnps from yn01 were characterized as pure face-centered cubic fe3o4 with an average size of 80 nm through transmission electron microscope (tem), x-ray diffraction (xrd), and x-ray photoelectron spectroscopy (xps). the hysteresis parameters of the bmnp samples such as bc and bcr and ratios mrs/ms wer ... | 2015 | 25030455 |
| naturally-occurring tetrahydro-β-carboline alkaloids derived from tryptophan are oxidized to bioactive β-carboline alkaloids by heme peroxidases. | β-carbolines are indole alkaloids that occur in plants, foods, and endogenously in mammals and humans, and which exhibit potent biological, psychopharmacological and toxicological activities. they form from naturally-occurring tetrahydro-β-carboline alkaloids arising from tryptophan by still unknown way and mechanism. results in this research show that heme peroxidases catalyzed the oxidation of tetrahydro-β-carbolines (i.e. 1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid and 1-methyl-1,2,3,4-t ... | 2014 | 25035927 |
| effect of local application of transforming growth factor-β at the nerve repair site following chronic axotomy and denervation on the expression of regeneration-associated genes. laboratory investigation. | although peripheral nerves can regenerate after traumatic injury, functional recovery is often suboptimal, especially after injuries to large nerve trunks such as the sciatic nerve or brachial plexus. current research with animal models suggests that the lack of functional recovery resides in the lack of sufficient mature axons reaching their targets due to the loss of neurotrophic support by schwann cells in the distal stump of injured nerves. this study was designed to investigate the effect o ... | 2014 | 25036208 |
| horseradish peroxidase-catalyzed formation of hydrogels from chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups. | horseradish peroxidase-catalyzed cross-linking was applied to prepare hydrogels from aqueous solutions containing chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups (denoted as ph-chitosan and ph-pva, respectively). comparing the hydrogels prepared from the solution of 1.0% (w/v) ph-chitosan and 3.0% (w/v) ph-pva and that of 3.0% (w/v) ph-chitosan and 1.0% (w/v) ph-pva, the gelation time of the former hydrogel was 47 s, while was 10s longer than that of the lat ... | 2014 | 25037368 |