Publications

direct interaction between terbium ion and peroxidase in horseradish at different ph values.	rare earth elements (rees) entering plant cells can directly interact with peroxidase in plants, which is the structural basis for the decrease in the activity of peroxidase. different cellular compartments have different ph values. however, little information is available regarding the direct interaction between rees and peroxidase in plants at different ph values. here, we investigated the charge distribution on the surface of horseradish peroxidase (hrp) molecule as well as the interaction of ...	2014	24420373
involvement of malate, monophenols, and the superoxide radical in hydrogen peroxide formation by isolated cell walls from horseradish (armoracia lapathifolia gilib.).	peroxidase associated with isolated horseradish cell walls catalyzes the formation of h2o2 in the presence of nadh. the reaction is stimulated by various monophenols, especially of coniferyl alcohol. nadh can be provided by a bound malate dehydrogenase. this system is capable of polymerizing coniferyl alcohol yielding an insoluble dehydrogenation polymer. nadh was found to be oxidized by two different mechanisms, one involving mn(2+), monophenol, and the superoxide radical o2 (·-) in a reaction ...	1977	24420401
protein-accumulating cells and dilated cisternae of the endoplasmic reticulum in three glucosinolate-containing genera: armoracia, capparis, drypetes.	three glucosinolate-containing species, armoracia rusticana gaertner, meyer et scherbius (brassicaceae), capparis cynophallophora l. (capparaceae) and drypetes roxburghii (wall.) hurusawa (euphorbiaceae), are shown by both light and electron microscopy to contain protein-accumulating cells (pac). the pac of armoracia and copparis (former "myrosin cells") occur as idioblasts. the pac of drypetes are usual members among axial phloem parenchyma cells rather than idioblasts. in drypetes the vacuoles ...	1977	24420656
formation of hydrogen peroxide by isolated cell walls from horseradish (armoracia lapathifolia gilib.).	isolated cell-wall suspensions from horseradish in the presence of 5×10(-4) m mncl2 catalyze the production of hydrogen peroxide at the expense of either nadph or nadh. this reaction is inhibited by scavengers of the superoxide free radical ion such as ascorbate or dihydroxyphenols or by superoxide dismutase, and stimulated by monophenols such as p-coumaric acid. on comparison with isolated (commercial) horseradish peroxidase it becomes evident that (a) cell-wall-bound peroxidase(s) is (are) res ...	1976	24424595
ethylene production by cress roots and excised cress root segments and its inhibition by 3,5-diiodo-4-hydroxybenzoic acid.	3,5-diiodo-4-hydroxybenzoic acid (dihb) has been shown to exert an inhibitory effect on the formation of ethylene by the roots of intact cress lepidium sativum seedlings in light, and by excised cress root segments. adding iaa to the culture solution greatly promoted ethylene production, which was suppressed by dihb. the findings together with results obtained with dinitrophenol (dnp), l-methionine and l-ethionine and also the horseradish peroxidase/methional system of yang are discussed.the res ...	1975	24430221
highly sensitive and quantitative human thrombospondin-1 detection by an m55 aptasensor and clinical validation in patients with atherosclerotic disease.	aptamer-based biosensors (aptasensor) are powerful tools for rapid and sensitive biomarker detection. in this study, we report a dna aptamer probe evolved from cell-selex that can recognize thrombospondin-1 protein in human plasma samples. the kd value of the aptamer m55 binding to thrombospondin-1 was determined as 0.5 ± 0.2 μm with an r(2) of 0.9144. a horseradish peroxidase-linked short oligo was complementarily bound onto the 3' end of the aptamer sequence to facilitate the 'smart' design of ...	2014	24434496
highly enhanced electrochemiluminescence based on pseudo triple-enzyme cascade catalysis and in situ generation of co-reactant for thrombin detection.	in this work, a novel pseudo triple-enzyme cascade catalysis amplification strategy was employed to fabricate a highly sensitive electrochemiluminescence (ecl) aptasensor for thrombin (tb) detection. the signal amplification of the proposed aptasensor was based on the synergistic catalysis of glucose dehydrogenase (gdh) and hemin/g-quadruplex to generate a co-reactant in situ for the ecl of peroxydisulfate. gold nanorods (aunrs) conjugated with gdh and hemin/g-quadruplex were used as the seconda ...	2014	24443720
production of monoclonal antibody for okadaic acid and its utilization in an ultrasensitive enzyme-linked immunosorbent assay and one-step immunochromatographic strip.	okadaic acid (oa) is a common marine biotoxin that accumulates in bivalves and causes diarrhetic shellfish poisoning (dsp). this study generated a monoclonal antibody (mab) specific to oa from a hybridoma cell line, 6b1a3, which was obtained by fusion of myeloma cells (p3/ns1/1-ag4-1) with spleen cells isolated from a balb/c mouse immunized with oa-γ-globulin. the 6b1a3 mab belongs to the immunoglobulin g1 (κ chain) isotype. both competitive direct and indirect enzyme-linked immunosorbent assays ...	2014	24446876
identification of plant metabolites of environmental contaminants by uplc-qtof-ms: the in vitro metabolism of triclosan in horseradish.	plants can extensively transform contaminants after uptake through phase i and phase ii metabolism to a large diversity of products. uplc-qtof-ms was used to detect and identify metabolites of the bacteriostatic agent triclosan in a horseradish hairy root culture. thirty-three metabolites of triclosan were recognized by a stepwise approach of mass defect filtering, multivariate data analysis, and isotope pattern filtering from a data set of several thousands of signals in the exposed culture. st ...	2014	24456336
quantum dot-functionalized porous zno nanosheets as a visible light induced photoelectrochemical platform for dna detection.	this work reports the synthesis of novel cdte quantum dot (qd)-functionalized porous zno nanosheets via a covalent binding method with (3-aminopropyl)triethoxysilane as a linker. the functional nanosheets showed an excellent visible-light absorbency and much higher photoelectrochemical activity than both cdte qds and zno nanosheets due to the porous structure and appropriate band alignment between the cdte qds and zno nanosheets. using hydrogen peroxide as an electron acceptor the nanosheet-modi ...	2014	24457595
multi-nanomaterial electrochemical biosensor based on label-free graphene for detecting cancer biomarkers.	developing a rapid, accurate and sensitive electrochemical biosensor for detecting cancer biomarkers is important for early detection and diagnosis. this work reports an electrochemical biosensor based on a graphene (gr) platform which is made by cvd, combined with magnetic beads (mbs) and enzyme-labeled antibody-gold nanoparticle bioconjugate. mbs coated with capture antibodies (ab1) were attached to gr sheets by an external magnetic field, to avoid reducing the conductivity of graphene. sensit ...	2014	24462797
revisiting the classification of curtoviruses based on genome-wide pairwise identity.	members of the genus curtovirus (family geminiviridae) are important pathogens of many wild and cultivated plant species. until recently, relatively few full curtovirus genomes have been characterised. however, with the 19 full genome sequences now available in public databases, we revisit the proposed curtovirus species and strain classification criteria. using pairwise identities coupled with phylogenetic evidence, revised species and strain demarcation guidelines have been instituted. specifi ...	2014	24463952
disulfide bond generation in mammalian blood serum: detection and purification of quiescin-sulfhydryl oxidase.	a sensitive new plate-reader assay has been developed showing that adult mammalian blood serum contains circulating soluble sulfhydryl oxidase activity that can introduce disulfide bonds into reduced proteins with the reduction of oxygen to hydrogen peroxide. the activity was purified 5000-fold to >90% homogeneity from bovine serum and found by mass spectrometry to be consistent with the short isoform of quiescin-sulfhydryl oxidase 1 (qsox1). this fad-dependent enzyme is present at comparable ac ...	2014	24468475
receptor expression is essential for forward motility in the course of sperm cell maturation.	forward motility stimulating factor (fmsf) is a glycoprotein previously purified from buffalo blood serum that promotes progressive motility of caprine caudal spermatozoa. we have prepared a functionally active covalent conjugate of this factor with horseradish-peroxidase (hrp) to obtain an idea of its binding efficacy on maturing spermatozoa. receptor-assay was performed using fmsf-hrp conjugate in saturating conditions to bind with spermatozoa isolated from different epididymal segments. activ ...	2014	24471917
flos lonicera ameliorates obesity and associated endotoxemia in rats through modulation of gut permeability and intestinal microbiota.	increasing evidence has indicated a close association of host-gut flora metabolic interaction with obesity. flos lonicera, a traditional herbal medicine, is used widely in eastern asia for the treatment of various disorders. the aim of this study was to evaluate whether unfermented or fermented formulations of flos lonicera could exert a beneficial impact to combat obesity and related metabolic endotoxemia.	2014	24475077
investigations of an electrochemical platform based on the layered mos2-graphene and horseradish peroxidase nanocomposite for direct electrochemistry and electrocatalysis.	the self-assembly of layered molybdenum disulfide-graphene (mos2-gr) and horseradish peroxidase (hrp) by electrostatic attraction into a novel hybrid nanomaterial (hrp-mos2-gr) is reported. the properties of the mos2-gr were characterized by x-ray diffraction (xrd), high-resolution transmission electron microscopy (tem), electrochemical impedance spectroscopy (eis) and cyclic voltammetry (cv). uv-vis and fourier transform infrared spectroscopy (ft-ir) indicate that the native structure of the hr ...	2014	24480133
chemiluminescent detection of cell apoptosis enzyme by gold nanoparticle-based resonance energy transfer assay.	we report a new chemiluminescence resonance energy transfer (cret) technique, using gold nanoparticles (aunps) as efficient energy acceptor, for homogeneous measurement of cell apoptosis enzyme with high sensitivity. in the design of the cret system, we chose the highly sensitive chemiluminescence (cl) reaction between luminol and hydrogen peroxide catalysed by horseradish peroxidase (hrp) because the cl spectrum of luminol (λ max 425 nm) partially overlaps the visible absorption bands of aunps. ...	2014	24481623
epr study of polyaniline synthesized enzymatically in the presence of submicrometer-sized aot vesicles.	epr spectroscopy was used to examine the magnetic properties of two enzymatically synthesized polyaniline (pani) samples obtained in the presence of submicrometer-sized vesicles formed from sodium bis(2-ethylhexyl)sulfosuccinate (aot) as templates. pani-hrpc-aot was synthesized with horseradish peroxidase isoenzyme c (hrpc) and hydrogen peroxide (h2o2) as oxidant while pani-tvl-aot was prepared with trametes versicolor laccase (tvl) and dioxygen (o2) as oxidant. a commercial conductive sample of ...	2014	24484357
the vtlisfg motif in the bh1 domain plays a significant role in regulating the degradation of mcl-1.	mcl-1 is a member of the bcl-2 family protein; its degradation is required for the initiation of apoptosis. the mechanism, however, is not yet clearly known. previously, it was reported that mcl-1 is degraded through the ubiquitination-mediated pathway and the pest domain is the motif responsible for promoting this degradation. we found evidence that this may not be true. we generated several mcl-1 deletion mutants and examined their effects on protein stability. deletion of the pest domain did ...	2014	24490139
absorbance enhancement in microplate wells for improved-sensitivity biosensors.	a generic optical biosensing strategy was developed that relies on the absorbance enhancement phenomenon occurring in a multiple scattering matrix. experimentally, inserts made of glass fiber membrane were placed into microplate wells in order to significantly lengthen the trajectory of the incident light through the sample and therefore increase the corresponding absorbance. enhancement factor was calculated by comparing the absorbance values measured for a given amount of dye with and without ...	2014	24491962
long-term dry storage of an enzyme-based reagent system for elisa in point-of-care devices.	lateral flow devices are commonly used for many point-of-care (poc) applications in low-resource settings. however, they lack the sensitivity needed for many analytes relevant in the diagnosis of diseases. one approach to achieve higher sensitivity is signal amplification, which is commonly used in laboratory assays, but uses reagents that require refrigeration and inherently requires multiple assay steps not normally compatible with poc settings. enzyme-based signal amplification, such as the o ...	2014	24496140
facile synthesis of boronic acid-functionalized magnetic carbon nanotubes for highly specific enrichment of glycopeptides.	a stepwise strategy was developed to synthesize boronic acid functionalized magnetic carbon nanotubes (mcnts) for highly specific enrichment of glycopeptides. the mcnts were synthesized by a solvothermal reaction of fe(3+) loaded on the acid-treated cnts and modified with 1-pyrenebutanoic acid n-hydroxysuccinimidyl ester (pase) to bind aminophenylboronic acid (apba) via an amide reaction. the introduction of pase could bridge the mcnt and apba, suppress the nonspecific adsorption and reduce the ...	2014	24496404
detoxification of pesticides aqueous solution using horseradish peroxidase.	there are pesticide residues in agriculture wastewater and that compounds must be removed before discharge of wastewater in native waters. thus the aim of this study was to remove toxic pesticide in waste water by the addition of horseradish peroxidase enzyme. the process of pesticide (methyl-parathion (o,o-diethyl- o-4-nitro-phenylthiophosphate), atrazine (1-chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) and triazophos (o,o-diethyl o-1-phenyl-1h-1,2,4- triazol-3-yl phosphorothioate) remov ...	2013	24498792
[influence of nogo extracellular peptide residues 1-40 gene modification on survival and differentiation of neural stem cells after transplantation].	to investigate the influence of nogo extracellular peptide residues 1-40 (nep1-40) gene modification on the survival and differentiation of the neural stem cells (nscs) after transplantation.	2013	24501899
analysis of telomerase by the telomeric hemin/g-quadruplex-controlled aggregation of au nanoparticles in the presence of cysteine.	telomeres are guanosine-rich nucleic-acid chains that fold, in the presence of k(+) ions and hemin, into the telomeric hemin/g-quadruplex structure, exhibiting horseradish peroxidase mimicking functions. the telomeric hemin/g-quadruplex structures catalyze the oxidation of thiols (e.g., l-cysteine) into disulfides (e.g., cystine). as l-cysteine stimulates the aggregation of au nanoparticles (nps), accompanied by absorbance changes from red (individual au nps) to blue (aggregated au nps), the pro ...	2014	24502233
a highly sensitive immunosensor for calmodulin assay based on enhanced biocatalyzed precipitation adopting a dual-layered enzyme strategy.	calmodulin (cam) is a ubiquitous protein in eukaryotic cells, and it plays an important role in cancer progression. in this paper, a highly sensitive immunosensor adopting a dual-layered enzyme strategy was proposed for electrochemical detection of cam. this immunosensor was constructed by introducing honeycomb-like mesoporous carbon (hmpc) as a sensor platform to sequentially immobilize antibody (ab1), cam and a multi-functionalized label. the label (hrp-paupc-ab1) was synthesized by covalently ...	2014	24508817
biosynthesis of n,n-dimethyltryptamine (dmt) in a melanoma cell line and its metabolization by peroxidases.	tryptophan (trp) is essential for many physiological processes, and its metabolism changes in some diseases such as infection and cancer. the most studied aspects of trp metabolism are the kynurenine and serotonin pathways. a minor metabolic route, tryptamine and n,n-dimethyltryptamine (dmt) biosynthesis, has received far less attention, probably because of the very low amounts of these compounds detected only in some tissues, which has led them to be collectively considered as trace amines. in ...	2014	24508833
asic3 in the cerebrospinal fluid-contacting nucleus of brain parenchyma contributes to inflammatory pain in rats.	the study explores the role of acid-sensitive ion channel 3 (asic3) in the cerebrospinal fluid (csf)-contacting nucleus of the parenchyma of rat brain, in inflammatory pain.	2014	24512021
autophagy of iron-binding proteins may contribute to the oxidative stress resistance of arpe-19 cells.	the objective of this study was to elucidate possible reasons for the remarkable resistance of human retinal pigment epithelial (rpe) cells to oxidative stress. much oxidative damage is due to hydrogen peroxide meeting redox-active iron in the acidic and reducing lysosomal environment, resulting in the production of toxic hydroxyl radicals that may oxidize intralysosomal content, leading to lipofuscin (lf) formation or, if more extensive, to permeabilization of lysosomal membranes. formation of ...	2013	24512774
a glance at the reactivity of osma(ii)cycles [os(c-n)x(bpy)3-x](m+) (x=0-3) covering a 1.8 v potential range toward peroxidase through monte carlo simulations ((-)c-n=o-2-phenylpyridinato, bpy=2,2'-bipyridine).	three cyclometalated and one coordination compounds [os(c-n)x(bpy)3-x](m) (x/m=0/2+ (4); 1/1+ (3); 2/1+ (2); 3/0 (1); (-)c-n=2-phenylpyridinato, bpy=2,2'-bipyridine) with drastically different reduction potentials have been used for analyzing the second-order rate constants for one-electron, metal-based osmium(ii) to osmium(iii) oxidation of the complexes by compound i (k2) and compound ii (k3) of horseradish peroxidase. previously unknown k2 and k3 have been determined by digital simulation of ...	2014	24513611
synthesis of an intein-mediated artificial protein hydrogel.	we present the synthesis of a highly stable protein hydrogel mediated by a split-intein-catalyzed protein trans-splicing reaction. the building blocks of this hydrogel are two protein block-copolymers each containing a subunit of a trimeric protein that serves as a crosslinker and one half of a split intein. a highly hydrophilic random coil is inserted into one of the block-copolymers for water retention. mixing of the two protein block copolymers triggers an intein trans-splicing reaction, yiel ...	2014	24514743
development of atom transfer radical polymer-modified gold nanoparticle-based enzyme-linked immunosorbent assay (elisa).	in this work, a novel enzyme-linked immunosorbent assay (elisa) with a low limit of detection and high sensitivity was developed using atom transfer radical polymer (atrp)-modified gold nanoparticles (aunps). clear signal amplification was achieved by introducing an abundance of horseradish peroxidase (hrp) to the aunps, because of the atrp modification. this result suggested that the new elisa was able to detect antigens in complex mixtures, and the limit of detection (lod) was lower than that ...	2014	24517078
facile co-assembly process to generate core-shell nanoparticles with functional protein corona.	a simple and robust protocol to maintain the structural feature of polymer-protein core-shell nanoparticles (ppcs-nps) is developed based on the synergistic interactions between proteins and functional polymers. using the self-assembly method, a broad range of proteins can be assembled to the selective water-insoluble polymers containing pyridine groups. the detailed analysis of the ppcs-nps structure was conducted using fesem and thin-sectioned tem. the results illustrated that the protein mole ...	2014	24517712
coupled enzyme reactions performed in heterogeneous reaction media: experiments and modeling for glucose oxidase and horseradish peroxidase in a peg/citrate aqueous two-phase system.	the intracellular environment in which biological reactions occur is crowded with macromolecules and subdivided into microenvironments that differ in both physical properties and chemical composition. the work described here combines experimental and computational model systems to help understand the consequences of this heterogeneous reaction media on the outcome of coupled enzyme reactions. our experimental model system for solution heterogeneity is a biphasic polyethylene glycol (peg)/sodium ...	2014	24517887
development of spr biosensor for the detection of human hepatitis b virus using plasma-treated parylene-n film.	a plasma-treated parylene-n film was presented for the immobilization of proteins through physical adsorption. the changes in surface properties of the parylene-n film after plasma-treatment were analyzed using contact angle microscopy and afm. to demonstrate the high protein-immobilization efficiency of the plasma-treated parylene-n film, the immobilization efficiencies of differently modified surfaces were compared using model proteins with different surface charges, such as streptavidin (pi=5 ...	2014	24518301
influence of immobilization protocol on the structure and function of surface bound proteins.	a new coupling strategy for biomacromolecules with (3-mercaptopropyl)trimethoxysilane (3mptms) and 11-(triethoxysilyl)undecanal (tesu) on gold surfaces is. this immobilization protocol was utilized for the enzyme horseradish peroxidase (hrp). to study the reactions and resulting structures, pm-irras measurements were performed. pm-irras shows there is structure preservation of the hrp when the new coupling strategy is used in contrast to non-specific adsorption on gold. the biological activity o ...	2014	24524936
synthesis of starch-g-p(dmdaac) using hrp initiation and the correlation of its structure and sludge dewaterability.	a new cationic starch used as a sludge dewatering agent was prepared by grafting copolymerization of degradation starch and dimethyldiallylammonium chloride (dmdaac) using horseradish peroxidase/h2o2 initiation. its chemical structure was characterized by ftir, (1)h nmr, (13)c nmr, gel permeation chromatography, graft percent, and graft efficiency. the results indicated that its structure was built by grafting the dmdaac oligomer onto the starch backbone as branched chains, with stronger hydroph ...	2014	24528731
effects of the green tea catechin (-)-epigallocatechin gallate on trypanosoma brucei.	the current pharmacopeia to treat the lethal human and animal diseases caused by the protozoan parasite trypanosoma brucei remains limited. the parasite's ability to undergo antigenic variation represents a considerable barrier to vaccine development, making the identification of new drug targets extremely important. recent studies have demonstrated that fatty acid synthesis is important for growth and virulence of trypanosoma brucei brucei, suggesting this pathway may have therapeutic potential ...	2012	24533284
specific probe selection from landscape phage display library and its application in enzyme-linked immunosorbent assay of free prostate-specific antigen.	probes against targets can be selected from the landscape phage library f8/8, displaying random octapeptides on the pviii coat protein of the phage fd-tet and demonstrating many excellent features including multivalency, stability, and high structural homogeneity. prostate-specific antigen (psa) is usually determined by immunoassay, by which antibodies are frequently used as the specific probes. herein we found that more advanced probes against free prostate-specific antigen (f-psa) can be scree ...	2014	24533565
polymerization reactivity of sulfomethylated alkali lignin modified with horseradish peroxidase.	alkali lignin (al) was employed as raw materials in the present study. sulfomethylation was conducted to improve the solubility of al, while sulfomethylated alkali lignin (sal) was further polymerized by horseradish peroxidase (hrp). hrp modification caused a significant increase in molecular weight of sal which was over 20 times. it was also found to increase the amount of sulfonic and carboxyl groups while decrease the amount of phenolic and methoxyl groups in sal. the adsorption quantity of s ...	2014	24534439
technical note: optimization of lactose quantification based on coupled enzymatic reactions.	a colorimetric microplate-adapted lactose assay was developed to quantify lactose in dairy products. the assay was based on the coupled enzymatic reaction of β-galactosidase-glucose oxidase-horseradish peroxidase using amplex red as detection probe. the assay showed good linearity in the range of 0.1 to 0.5mmol of lactose/l, with a limit of detection of 0.0433mmol/l and a limit of quantification of 0.1313mmol/l. the lactose assay at optimized conditions (5 u of β-galactosidase/ml, 5 u of glucose ...	2014	24534513
aptamer-based microfluidic beads array sensor for simultaneous detection of multiple analytes employing multienzyme-linked nanoparticle amplification and quantum dots labels.	this study reports the development of an aptamer-mediated microfluidic beads-based sensor for multiple analytes detection and quantification using multienzyme-linked nanoparticle amplification and quantum dots labels. adenosine and cocaine were selected as the model analytes to validate the assay design based on strand displacement induced by target-aptamer complex. microbeads functionalized with the aptamers and modified electron rich proteins were arrayed within a microfluidic channel and were ...	2014	24534576
ultra-sensitive, high-throughput detection of infectious diarrheal diseases by portable chemiluminescence imaging.	this paper describes a rapid, ultra-sensitive, and high-throughput pathogenic dna identification strategy for infectious diarrheal diseases diagnosis. this strategy is based on specific dna hybridization and horseradish-peroxidase-catalyzed chemiluminescence (cl) detection. probe dna strands are covalently immobilized on the aldehyde-group-modified slide and hybridized with biotin-modified target dna strands. horseradish-peroxidase (hrp) is then combined with the target dna via a biotin-streptav ...	2014	24534578
bioelectrochemical systems with oleylamine-stabilized gold nanostructures and horseradish peroxidase for hydrogen peroxide sensor.	this paper describes ultrathin gold nanowires (nws) and nanoparticles (nps) prepared by oleylamine (oa) synthesis and their assembly with horseradish peroxidase enzyme (hrp) for bioelectrochemical sensing of hydrogen peroxide for the first time. the immobilization of oxidoreductase enzyme hrp on the electrodes modified with oa gold nanostructures (oanss) is discussed. the hrp-sensor characteristics, namely sensitivity, working concentration range, sensor-to-sensor and measurement-to-measurement ...	2014	24534581
mechanistic insight into sono-enzymatic degradation of organic pollutants with kinetic and thermodynamic analysis.	in this paper, we have attempted to get a physical insight into process of sono-enzymatic treatment for degradation of recalcitrant organic pollutants. decolourization of an azo dye has been used as model reaction with different experimental protocols that alter characteristics of ultrasound and cavitation phenomena in the system. experimental data is analyzed to determine kinetic and thermodynamic parameters of decolorization process. the trends observed in kinetic and thermodynamic parameters ...	2014	24548544
monodispersed silica nanoparticles as carrier for co-immobilization of bi-enzyme and its application for glucose biosensing.	a novel glucose sensing strategy by using bi-enzyme coated monodispered silica nanoparticles (sio2) was proposed. the monodispered sio2 was synthesized according to our previously reported seed-growth methods. glucose oxidase (god) and horseradish peroxidase (hrp) were simultaneously covalent immobilized on the surface of sio2 nanoparticles through the cross-linker of glutaraldehyde. the immobilized bi-enzyme remained their bioactivities well for the substrate reaction. thus, the resultant sio2- ...	2014	24548811
an in vitro spectrophotometric analysis of the penetration of bleaching agent into the pulp chamber of intact and restored teeth.	to investigate the pulp chamber penetration of bleaching agent in intact teeth and teeth following restorative procedure.	2013	24551727
small molecule-initiated light-activated semiconducting polymer dots: an integrated nanoplatform for targeted photodynamic therapy and imaging of cancer cells.	photodynamic therapy (pdt) is a noninvasive and light-activated method for cancer treatment. two of the vital parameters that govern the efficiency of pdt are the light irradiation to the photosensitizer and visual detection of the selective accumulation of the photosensitizer in malignant cells. herein, we prepared an integrated nanoplatform for targeted pdt and imaging of cancer cells using folic acid and horseradish peroxidase (hrp)-bifunctionalized semiconducting polymer dots (fh-pdots). in ...	2014	24552272
enhanced chemiluminescence of cdte quantum dots-h₂o₂ by horseradish peroxidase-mimicking dnazyme.	in this study, it was found that horseradish peroxidase (hrp)-mimicking dnazyme could effectively enhance the cl emission of cdte quantum dots (qds)-h2o2 system, whereas hrp could not enhance the cl intensity. the cl enhancement mechanism was investigated, and the cl enhancement was supposed to originate from the catalysis of hrp-mimicking dnazyme on the cl reaction between cdte qds and h2o2. meantime, compared with cdte qds-h2o2 cl system, h2o2 concentration was markedly decreased in qds-h2o2-h ...	2014	24556131
modification of lysine residues of horseradish peroxidase and its effect on stability and structure of the enzyme.	biotechnology is consistently seeking improved enzyme stability. enzymes have great properties, although their marginal stability limits their applications. among the strategies for improving stability of the enzymes, chemical modification is a simple and effective technique. in the present study, chemical modification of horseradish peroxidase (hrp) was carried out with 2,3-dichloromaleic anhydride and 2,3-dimethylmaleic anhydride. hrp is an important heme-containing enzyme. it is widely applie ...	2014	24557950
amperometric carbohydrate antigen 19-9 immunosensor based on three dimensional ordered macroporous magnetic au film coupling direct electrochemistry of horseradish peroxidase.	a sandwich-type electrochemical immunosensor for the detection of carbohydrate antigen 19-9 (ca 19-9) antigen based on the immobilization of primary antibody (ab1) on three dimensional ordered macroporous magnetic (3domm) electrode, and the direct electrochemistry of horseradish peroxidase (hrp) that was used as both the label of secondary antibody (ab2) and the blocking reagent. the 3domm electrode was fabricated by introducing core-shell au-sio2@fe3o4 nanospheres onto the surface of three dime ...	2014	24560371
enzymatic conjugation of a bioactive peptide into an injectable hyaluronic acid-tyramine hydrogel system to promote the formation of functional vasculature.	in this study, one-step enzyme-mediated preparation of a multi-functional injectable hyaluronic-acid-based hydrogel system is reported. hydrogel was formed through the in situ coupling of phenol moieties by horseradish peroxidase (hrp) and hydrogen peroxide (h2o2), and bioactive peptides were simultaneously conjugated into the hydrogel during the gel formation process. the preparation of this multi-functional hydrogel was made possible by synthesizing peptides containing phenols which could coup ...	2014	24561710
reactions of amino acids, peptides, and proteins with oxidized metabolites of tris(p-carboxyltetrathiaaryl)methyl radical epr probes.	oxidation of the tris(p-carboxyltetrathiaaryl)methyl (tam) epr radical probe, tama(•), by rat liver microsomes (rlm) + nadph, or horseradish peroxidase (hrp) + h2o2, or k2ircl6, led to an intermediate cation, tama(+), which was treated with glutathione (gsh), with formation of an adduct, tama-sg(•), resulting from the substitution of a tama(•) carboxylate group with the sg group. l-α-amino acids containing a strong nucleophilic residue (nuh), such as l-cysteine or l-histidine, also reacted with ...	2014	24564180
novel method for detection of reactive oxygen species in vivo in human skeletal muscle.	excessive production of reactive oxygen species (ros) are implicated in the pathogenesis of numerous disease states. however, direct measurement of in vivo ros in humans has remained elusive due to limited access to appropriate tissue beds and the inherently short half-lives and high reactivity of ros. herein, we describe a novel technique by which to measure in vivo ros in human skeletal muscle. microdialysis probes were inserted into the vastus lateralis of eight healthy volunteers. amplex ult ...	2014	24564604
an amperometric biosensor based on horseradish peroxidase immobilized onto maize tassel-multi-walled carbon nanotubes modified glassy carbon electrode for determination of heavy metal ions in aqueous solution.	a biosensor for trace metal ions based on horseradish peroxidase (hrp) immobilized on maize tassel-multiwalled carbon nanotube (mt-mwcnt) through electrostatic interactions is described herein. the biosensor was characterized using fourier transform infrared (ftir), uv-vis spectrometry, voltammetric and amperometric methods. the ftir and uv-vis results inferred that hrp was not denatured during its immobilization on mt-mwcnt composite. the biosensing principle was based on the determination of t ...	2014	24564899
[dendritic projections from meridian-related motoneurons to sympathic preganglion neurons in the spinal cord of rats].	previous studies indicated a close involvement of reflex activities of motoneurons in the spinal cord in the mechanism of meridian phenomena. the present study was designed to investigate the dendrite projections of meridian-related motoneurons among the motoneurons and sympathetic preganglionic neurons in the spinal cord.	2013	24579358
[establishment of a detection method for hepatitis b virus large surface protein (hbv-lp)].	to establish enzyme-linked immunosorbent assay (elisa) for detection of hepatitis b virus large surface protein(hbv-lp) in serum.	2013	24579479
[microplate chemiluminescence enzyme immunoassay for the quantitative analysis of tissue inhibitor of metalloproteinases i].	to establish microplate chemiluminescence enzyme immunoassay (cleia) for quantitative analysis of tissue inhibitor of metalloproteinases i (timp i) in human serum.	2013	24579481
effect of allyl isothiocyanate on ultra-structure and the activities of four enzymes in adult sitophilus zeamais.	rarefaction and vacuolization of the mitochondrial matrix of aitc-treated (allyl isothiocyanate-treated) adult sitophilus zeamais were evident according to the ultra-structural by tem. four important enzymes in adult s. zeamais were further studied after fumigation treatment with allyl isothiocyanate (aitc) extracted from armoracia rusticana roots and shoots. the enzymes were glutathione s-transferase (gst), catalase (cat), cytochrome c oxidase, and acetylcholinesterase (ache). the results indic ...	2014	24581380
changes in intestinal glucocorticoid sensitivity in early life shape the risk of epithelial barrier defect in maternal-deprived rats.	glucocorticoids (gc) contribute to human intestine ontogeny and accelerate gut barrier development in preparation to birth. rat gut is immature at birth, and high intestinal gc sensitivity during the first two weeks of life resembles that of premature infants. this makes suckling rats a model to investigate postpartum impact of maternal separation (ms)-associated gc release in preterm babies, and whether gc sensitivity may shape ms effects in immature gut. a 4 hours-ms applied once at postnatal ...	2014	24586321
a fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection.	most of the current colorimetric methods for detection of chitinase or cellulase activities on the insoluble natural polymers chitin and cellulose depend on a chemical redox reaction. the reaction involves the reducing ends of the hydrolytic products. the schales' procedure and the 3,5-dinitrosalicylic acid (dns) method are two examples that are commonly used. however, these methods lack sensitivity and present practical difficulties of usage in high-throughput screening assays as they require b ...	2014	24612932
engineering of pyranose dehydrogenase for increased oxygen reactivity.	pyranose dehydrogenase (pdh), a member of the gmc family of flavoproteins, shows a very broad sugar substrate specificity but is limited to a narrow range of electron acceptors and reacts extremely slowly with dioxygen as acceptor. the use of substituted quinones or (organo)metals as electron acceptors is undesirable for many production processes, especially of food ingredients. to improve the oxygen reactivity, site-saturation mutagenesis libraries of twelve amino acids around the active site o ...	2014	24614932
ultrasensitive opto-microfluidic immunosensor integrating gold nanoparticle-enhanced chemiluminescence and highly stable organic photodetector.	the expensive fabrication of current opto-microfluidic sensors is a barrier to the successful adoption of these devices in point-of-care testing. this work reports a simple inexpensive opto-microfluidic device incorporating a poly(dimethylsiloxane)-glass hybrid microfluidic chip modified with gold nanoparticles and a high-detectivity, high-stability organic photodetector. the enhancing effect of the gold nanoparticles on horseradish peroxidase-luminol-h2o2 chemiluminescence was exploited in rapi ...	2014	24615640
preparation of immobilized l-prolinamide via enzymatic polymerization of phenolic l-prolinamide and evaluation of its catalytic performance for direct asymmetric aldol reaction.	phenolic l-prolinamide was allowed to participate in enzymatic polymerization with horseradish peroxidase as the catalyst, generating immobilized l-prolinamide. the catalytic performance of the resultant polymer-supported l-prolinamide for direct asymmetric aldol reaction between aromatic aldehyde and cyclohexanone was studied. the results show that as prepared l-prolinamide can catalyze the aldol reaction at room temperature in the presence of h2o. relevant aldol addition products are obtained ...	2014	24619918
densified electrochemical sensors based on local redox cycling between vertically separated electrodes in substrate generation/chip collection and extended feedback modes.	a new local redox cycling-based electrochemical (lrc-ec) device integrated with many electrochemical sensors has been developed into a small chip device. the lrc-ec chip device was successfully applied for detection of alkaline phosphatase and horseradish peroxidase activity in substrate generation/chip collection (sg/cc) and extended feedback modes, respectively. the new imaging approach with extended feedback mode was particularly effective for sharpening of the image, because this mode uses f ...	2014	24621106
a novel horseradish peroxidase biosensor towards the detection of dopamine: a voltammetric study.	a polymerized film of glycine (gly) was prepared on the surface of carbon paste electrode (cpe) through the cyclic voltammetry (cv) technique. a novel biosensor for the determination of dopamine (da) has been constructed based on horseradish peroxidase (hrp) and multiwalled carbon nanotubes (mwcnts) immobilizing on poly (gly)/cpe through silica sol-gel (sisg) entrapment. cv measurements were employed in order to understand the feasibility of poly (gly) as an electron carrier between the immobili ...	2014	24629262
optimization of condition for conjugation of enrofloxacin to enzymes in chemiluminescence enzyme immunoassay.	in this study, in order to find out a proper method for conjugation of enrofloxacin to label enzymes, two methods were compared and carbodiimide condensation was proved to be better. the results showed that the binding ratio of enrofloxacin and alkaline phosphatase (alp) was 8:1 and that of enrofloxacin and horseradish peroxidase (hrp) was 5:1. this indicated that conjugate synthesized by carbodiimide condensation was fit for chemiluminescence enzyme immunoassay (cleia). furthermore, data reveal ...	2014	24632155
mechanism of reaction of chlorite with mammalian heme peroxidases.	this study demonstrates that heme peroxidases from different superfamilies react differently with chlorite. in contrast to plant peroxidases, like horseradish peroxidase (hrp), the mammalian counterparts myeloperoxidase (mpo) and lactoperoxidase (lpo) are rapidly and irreversibly inactivated by chlorite in the micromolar concentration range. chlorite acts as efficient one-electron donor for compound i and compound ii of mpo and lpo and reacts with the corresponding ferric resting states in a bip ...	2014	24632343
amorphous carbon nanoparticle used as novel resonance energy transfer acceptor for chemiluminescent immunoassay of transferrin.	amorphous carbon nanoparticles (acnps) showing highly efficient quenching of chemiluminescence (cl) were prepared from candle soot with a very simple protocol. the prepared acnp was employed as the novel energy acceptor for a chemiluminescence resonance energy transfer (cret)-based immunoassay. in this work, acnp was linked with transferrin (trf), and horseradish peroxidase (hrp) was conjugated to trf antibody (hrp-anti-trf). the immunoreaction rendered the distance between the acnp acceptor and ...	2014	24636417
extract from armoracia rusticana and its flavonoid components protect human lymphocytes against oxidative damage induced by hydrogen peroxide.	dna damage prevention is an important mechanism involved in cancer prevention by dietary compounds. armoracia rusticana is cultivated mainly for its roots that are used in the human diet as a pungent spice. the roots represent rich sources of biologically active phytocompounds, which are beneficial for humans. in this study we investigated the modulation of h₂o₂ genotoxicity using the a. rusticana root aqueous extract (ae) and two flavonoids (kaempferol or quercetin). human lymphocytes pre-treat ...	2014	24637991
[establishment of a quantitative detection method for golgi protein73].	to establish enzyme-linked immunosorbent assay (elisa) for quantitative detection of golgi protein73 (gp73) in serum.	2013	24645330
[quantitative detection of serum procollagen iii n-terminal peptide chemiluminescence enzyme immunoassay].	to establish chemiluminescence enzyme immunoassay (cleia) for quantitative detection of procollagen iii n-terminal peptide (p iii np) in serum.	2013	24645331
a dna origami nanorobot controlled by nucleic acid hybridization.	a prototype for a dna origami nanorobot is designed, produced, and tested. the cylindrical nanorobot (diameter of 14 nm and length of 48 nm) with a switchable flap, is able to respond to an external stimulus and reacts by a physical switch from a disarmed to an armed configuration able to deliver a cellular compatible message. in the tested design the robot weapon is a nucleic acid fully contained in the inner of the tube and linked to a single point of the internal face of the flap. upon actuat ...	2014	24648163
supramolecular assemblies of nucleoside functionalized carbon nanotubes: synthesis, film preparation, and properties.	nucleoside-functionalized multi-walled carbon nanotubes (n-mwcnts) were synthesized and characterized. a self-organization process using hydrogen bonding interactions was then used for the fabrication of self-assembled n-mwcnts films free of stabilizing agents, polymers, or surfactants. membranes were produced by using a simple water-dispersion-based vacuum-filtration method. hydrogen-bond recognition was confirmed by analysis with ir spectroscopy and tem images. restoration of the electronic co ...	2014	24648250
ursolic acid, a natural pentacyclic triterpenoid, inhibits intracellular trafficking of proteins and induces accumulation of intercellular adhesion molecule-1 linked to high-mannose-type glycans in the endoplasmic reticulum.	ursolic acid (3β-hydroxy-urs-12-en-28-oic acid) is a natural pentacyclic triterpenoid that is present in many plants, including medicinal herbs, and foods. ursolic acid was initially identified as an inhibitor of the expression of intercellular adhesion molecule-1 (icam-1) in response to interleukin-1α (il-1α). we report here a novel biological activity: ursolic acid inhibits intracellular trafficking of proteins. ursolic acid markedly inhibited the il-1α-induced cell-surface icam-1 expression i ...	2014	24649404
expression of human apolipoprotein e4 reduces insulin-receptor substrate 1 expression and akt phosphorylation in the ageing liver.	the diabetic drug rosiglitazone was reported to improve glucose tolerance in insulin-resistant apoe3 but not apoe4 knock-in mice. we therefore examined whether apolipoprotein e (apoe) has genotype-specific effects on liver insulin function. at 12 weeks, no difference in liver insulin signaling was detected between fasting apoe3 and apoe4 mice. at 72 weeks however, apoe4 mice had lower irs-1 and pi3k expression, and reduced akt phosphorylation. this decline was associated with lower insulin and h ...	2014	24649407
thin-layer polymer wrapped enzymes encapsulated in hierarchically mesoporous silica with high activity and enhanced stability.	a novel soft-hard cooperative approach was developed to synthesize bioactive mesoporous composite by pre-wrapping penicillin g amidase with poly(acrylaimde) nanogel skin and subsequently incorporating such penicillin g amidase nanocapsules into hierarchically mesoporous silica. the as-received bioactive mesoporous composite exhibited comparable activity and extraordinarily high stability in comparison with native penicillin g amidase and could be used repetitively in the water-medium hydrolysis ...	2014	24651701
development of enzyme-linked immunosorbent assay for estimation of urinary albumin.	yearly estimation of urinary albumin is a prerequisite for predicting renal status in diabetes type ii patients with negative dipstick results for overt proteinuria. a simple, sensitive, and cost-effective enzyme linked immunosorbent assay (elisa) for urinary albumin has been developed using human serum albumin antiserum (hsa-antiserum), hsa-biotin, and streptavidin-horseradish peroxidase (sa-hrp) conjugates. to the antibody-coated wells, 100 μl of hsa standards followed by 1:100 diluted urine s ...	2014	24654825
homogeneous assay of target molecules based on chemiluminescence resonance energy transfer (cret) using dnazyme-linked aptamers.	we have designed a single-stranded dnazyme-aptamer sensor for homogeneous target molecular detection based on chemiluminescence resonance energy transfer (cret). the structure of the engineered single-stranded dna (ssdna) includes the horseradish peroxidase (hrp)-like dnazyme, optimum-length linker (10-mer-length dna), and target-specific aptamer sequences. a quencher dye was modified at the 3' end of the aptamer sequence. the incorporation of hemin into the g-quadruplex structure of dnazyme yie ...	2014	24658027
hemoprotein-based supramolecular assembling systems.	hemoproteins are metalloproteins which include iron porphyrin as a cofactor. these proteins have received much attention as promising building blocks for development of new types of biomaterials. this review summarizes recent efforts in the rational design of supramolecular hemoprotein assemblies using myoglobin, horseradish peroxidase, cytochrome b562 and cytochrome c as a monomer unit. the processes of coordination bond-mediated assembly or domain swapping-mediated assembly provide defined oli ...	2014	24658057
all-water-based electron-beam lithography using silk as a resist.	traditional nanofabrication techniques often require complex lithographic steps and the use of toxic chemicals. to move from the laboratory scale to large scales, nanofabrication should be carried out using alternative procedures that are simple, inexpensive and use non-toxic solvents. recent efforts have focused on nanoimprinting and the use of organic resists (such as quantum dot-polymer hybrids, dna and poly(ethylene glycol)), which still require, for the most part, noxious chemicals for proc ...	2014	24658173
in vivo effects of 17β-estradiol on cardiac na(+)/k(+)-atpase expression and activity in rat heart.	in this study the in vivo effects of estradiol in regulating na(+)/k(+)-atpase function in rat heart was studied. adult male wistar rats were treated with estradiol (40μg/kg, i.p.) and after 24h the animals were sacrificed and the heart excised. following estradiol administration, cardiac na(+)/k(+)-atpase activity, expression of the α1 subunit, and phosphorylation of the α1 subunit were significantly increased. these animals also had significantly decreased levels of digoxin-like immunoreactive ...	2014	24662727
bisretinoid degradation and the ubiquitin-proteasome system.	bisretinoid fluorophores of retinal pigment epithelial (rpe) lipofuscin have been shown to undergo degradation in two ways, the first involving photofragmentation following photooxidation of their polyene structure and the second being enzyme-mediated and limited, thus far, to in vitro models employing horseradish peroxidase (hrp). here we show that both of these processes impact the ubiquitin-proteasome system (ups) of the rpe cell. by measuring the consumption of a2e and all-trans-retinal dime ...	2014	24664748
peroxidase gene discovery from the horseradish transcriptome.	horseradish peroxidases (hrps) from armoracia rusticana have long been utilized as reporters in various diagnostic assays and histochemical stainings. regardless of their increasing importance in the field of life sciences and suggested uses in medical applications, chemical synthesis and other industrial applications, the hrp isoenzymes, their substrate specificities and enzymatic properties are poorly characterized. due to lacking sequence information of natural isoenzymes and the low levels o ...	2014	24666710
secretion-related structures of hypothalamo-hypophysial terminals in the rat posterior pituitary.	hypothalamic terminals were investigated in the rat posterior pituitary (pp). injection of wheat germ agglutinin conjugated horseradish peroxidase (wga-hrp) and co-injection of wga-hrp with rab3a-sirna were made into the hypothalamus, respectively. additional injection of wga-hrp was made into the hypothalamus in the animals exposed to ethanol. these injections resulted in heavy labeling of fibers exclusively confined to the pp. ultrastructural observations showed terminals, fibers, pituicytes, ...	2013	24670492
expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains.	lipid rafts that are enriched in glycosylphosphatidylinositol (gpi)-anchored proteins serve as a platform for important biological events. to elucidate the molecular mechanisms of these events, identification of co-clustering molecules in individual raft domains is required. here we describe an approach to this issue using the recently developed method termed enzyme-mediated activation of radical source (emars), by which molecules in the vicinity within 300 nm from horseradish peroxidase (hrp) s ...	2014	24671047
western blotting using chemiluminescent substrates.	western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture (towbin et al., 1979). the technique enables indirect detection of protein samples immobilized on a nitrocellulose or polyvinylidene fluoride (pvdf) membrane.	2014	24674076
slow biotransformation of carbon nanotubes by horseradish peroxidase.	due to steady increase in use and mass production carbon nanotubes (cnts) will inevitably end up in the environment. because of their chemical nature cnts are expected to be recalcitrant and biotransform only at very slow rates. degradation of cnts within days has recently been reported, but excluding one study, conclusions relied solely on qualitative results. we incubated 13 different types of cnts and subjected them to enzymatic oxidation with horseradish peroxidase and concluded that the ana ...	2014	24678632
the effects of superoxide dismutase mimetic mntmpyp on the altered blood-brain barrier integrity in experimental preeclampsia with or without seizures in rats.	we investigated the effects of a cell-permeable superoxide dismutase mimetic, manganese(iii) tetrakis(1-methyl-4-pyridyl)porphyrin (mntmpyp) on blood-brain barrier (bbb) integrity following pentylenetetrazole (ptz)-induced seizures in experimental preeclampsia symptoms induced by n(omega)-nitro-l-arginine methyl ester (l-name) in pregnant rats. to show the functional and morphological alterations in bbb integrity, quantitative analysis of sodium fluorescein (naflu) extravasation, immunohistochem ...	2014	24680906
ion-responsive hemin-g-quadruplexes for switchable dnazyme and enzyme functions.	programmed nucleic acid sequences undergo k(+) ion-induced self-assembly into g-quadruplexes and separation of the supramolecular structures by the elimination of k(+) ions by crown ether or cryptand ion-receptors. this process allows the switchable formation and dissociation of the respective g-quadruplexes. the different g-quadruplex structures bind hemin, and the resulting hemin-g-quadruplex structures reveal horseradish peroxidase dnazyme catalytic activities. the following k(+) ion/receptor ...	2014	24683003
production and purification of the multifunctional enzyme horseradish peroxidase.	the oxidoreductase horseradish peroxidase (hrp) is used in numerous industrial and medical applications. in this review, we briefly describe this well-studied enzyme and focus on its promising use in targeted cancer treatment. in combination with a plant hormone, hrp can be used in specific enzyme-prodrug therapies. despite this outstanding application, hrp has not found its way as a biopharmaceutical into targeted cancer therapy yet. the reasons therefore lie in the present low-yield production ...	2013	24683473
sensitive electrochemical aptamer biosensor for dynamic cell surface n-glycan evaluation featuring multivalent recognition and signal amplification on a dendrimer-graphene electrode interface.	we demonstrate a multivalent recognition and highly selective aptamer signal amplification strategy for electrochemical cytosensing and dynamic cell surface n-glycan expression evaluation by the combination of concanavalin a (con a), a mannose binding protein, as a model, conjugated poly(amidoamine) dendrimer on a chemically reduced graphene oxide (rgo-den) interface, and aptamer- and horseradish peroxidase-modified gold nanoparticles (hrp-aptamer-aunps) as nanoprobes. in this strategy, the rgo- ...	2014	24684138
hierarchical hybrid peroxidase catalysts for remediation of phenol wastewater.	we report a new family of hierarchical hybrid catalysts comprised of horseradish peroxidase (hrp)-magnetic nanoparticles for advanced oxidation processes and demonstrate their utility in the removal of phenol from water. the immobilized hrp catalyzes the oxidation of phenols in the presence of h2 o2 , producing free radicals. the phenoxy radicals react with each other in a non-enzymatic process to form polymers, which can be removed by precipitation with salts or condensation. the hybrid peroxid ...	2014	24692298
enzyme encapsulated hollow silica nanospheres for intracellular biocatalysis.	hollow silica nanospheres (hsn) with low densities, large interior spaces and permeable silica shells are suitable for loading enzymes in the cavity to carry out intracellular biocatalysis. the porous shell can protect the encapsulated enzymes against proteolysis and attenuate immunological response. we developed a microemulsion-templating method for confining horseradish peroxidase (hrp) in the cavity of hsn. this simple one-pot enzyme encapsulation method allows entrapping of the enzyme, which ...	2014	24694065
dna sensors and aptasensors based on the hemin/g-quadruplex-controlled aggregation of au nps in the presence of l-cysteine.	l-cysteine induces the aggregation of au nanoparticles (nps), resulting in a color transition from red to blue due to interparticle plasmonic coupling in the aggregated structure. the hemin/g-quadruplex horseradish peroxidase-mimicking dnazyme catalyzes the aerobic oxidation of l-cysteine to cystine, a process that inhibits the aggregation of the nps. the degree of inhibition of the aggregation process is controlled by the concentration of the dnazyme in the system. these functions are implement ...	2014	24700798
gold-ish: a nano-size gold particle-based phylogenetic identification compatible with nanosims.	the linkage of microbial phylogenetic and metabolic analyses by combining ion imaging analysis with nano-scale secondary ion mass spectrometry (nanosims) has become a powerful means of exploring the metabolic functions of environmental microorganisms. phylogenetic identification using nanosims typically involves probing by horseradish peroxidase-mediated deposition of halogenated fluorescent tyramides, which permits highly sensitive detection of specific microbial cells. however, the methods req ...	2014	24702906
mechanism and regulation of peroxidase-catalyzed nitric oxide consumption in physiological fluids: critical protective actions of ascorbate and thiocyanate.	catalytic consumption of nitric oxide (no) by myeloperoxidase and related peroxidases is implicated as playing a key role in impairing no bioavailability during inflammatory conditions. however, there are major gaps in our understanding of how peroxidases consume no in physiological fluids, in which multiple reactive enzyme substrates and antioxidants are present. notably, ascorbate has been proposed to enhance myeloperoxidase-catalyzed no consumption by forming no-consuming substrate radicals. ...	2014	24704973
central pupillary light reflex circuits in the cat: i. the olivary pretectal nucleus.	the central pathways subserving the feline pupillary light reflex were examined by defining retinal input to the olivary pretectal nucleus (opt), the midbrain projections of this nucleus, and the premotor neurons within it. unilateral intravitreal wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp) injections revealed differences in the pattern of retinal opt termination on the two sides. injections of wga-hrp into opt labeled terminals bilaterally in the anteromedian nucleus, and ...	2014	24706328
electrochemical immunosensor based on hydrophilic polydopamine-coated prussian blue-mesoporous carbon for the rapid screening of 3-bromobiphenyl.	a sensitive electrochemical immunosensor for 3-bromobiphenyl (3-bbp) detection was constructed by employing a new polydopamine coated prussian blue-mesoporous carbon (pdop/pb/cmk-3) nanocomposite as the substrate platform and multi-horseradish peroxidase-double helix carbon nanotubes-secondary antibody (multi-hrp-dhcnts-ab2) as the signal label. pb/cmk-3 was firstly successfully in-situ synthesized with the aid of the cmk-3 reduction, which was characterized by transmission electron microscope ( ...	2014	24709325
an optical sensor for pesticide determination based on the autoindicating optical properties of peroxidase.	during the enzymatic reaction of the heme-protein horseradish peroxidase (hrp) with hydrogen peroxide there are changes in the molecular absorption spectra of hrp and its different oxidation states which can be used for quantitative determination of the substrate. one of these intermediate oxidation states is the hrpii, with iron as an oxyferryl. this compound is assumed to be responsible for the organophosphate pesticide degradation in the fenton reaction. in this work, the enzymatic hrp-h₂o₂ r ...	2014	24720992