Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| development of a direct elisa based on carboxy-terminal of penicillin-binding protein blar for the detection of β-lactam antibiotics in foods. | β-lactam antibiotics, including penicillins and cephalosporins, are commonly used in veterinary medicine. illegal use and abuse of β-lactams could cause allergy and selected bacterial resistance. blar-ctd, the carboxy-terminal of penicillin-recognizing protein blar from bacillus licheniformis atcc 14580, was utilized in this study to develop a receptor-based elisa for detection and determination of β-lactam antibiotics in milk, beef, and chicken. this assay was based on directly competitive inhi ... | 2013 | 24013636 |
| a highly sensitive prostate-specific antigen immunosensor based on gold nanoparticles/pamam dendrimer loaded on mwcnts/chitosan/ionic liquid nanocomposite. | we have developed a sensitive electrochemical immunosensor for the detection of prostate-specific antigen (psa), based on covalently immobilizing of anti-psa and redox mediator (thionine) onto gold nanoparticles-incorporated polyamidoamine dendrimer (aunps-pamam) and multiwalled carbon nanotubes/ionic liquid/chitosan nanocomposite (mwcnts/il/chit) as the support platform. the mwcnts/il/chit nanocomposite and synthesized aunps were characterized using sem and tem microscopy techniques. greatly am ... | 2014 | 24016535 |
| [the organization of the projections of the rostromedial tegmental nucleus to the striatum in canine brain]. | method of retrograde axonal transport of horseradish peroxidase and fluorochromes was used to study the organization of the projections of the rostromedial tegmental nucleus (rmtn) to the functionally diverse regions of striatal structures of dog brain (n=26). it was found that the projection fibers of the neurons of rmtn limbic medial part were directed only to the ventral limbic segments of the nucleus caudatus, the putamen and the nucleus accumbens. however, the convergence of the projection ... | 2013 | 24020179 |
| structural and functional characterization of the single-chain fv fragment from a unique hcv e1e2-specific monoclonal antibody. | the nucleotide sequence of the unique neutralizing monoclonal antibody d32.10 raised against a conserved conformational epitope shared between e1 and e2 on the serum-derived hepatitis c virus (hcv) envelope was determined. subsequently, the recombinant single-chain fv fragment (scfv) was cloned and expressed in escherichia coli, and its molecular characterization was assessed using multi-angle laser light scattering. the scfv mimicked the antibody in binding to the native serum-derived hcv parti ... | 2013 | 24021643 |
| a quantitative assay to study protein:dna interactions, discover transcriptional regulators of gene expression, and identify novel anti-tumor agents. | many dna-binding assays such as electrophoretic mobility shift assays (emsa), chemiluminescent assays, chromatin immunoprecipitation (chip)-based assays, and multiwell-based assays are used to measure transcription factor activity. however, these assays are nonquantitative, lack specificity, may involve the use of radiolabeled oligonucleotides, and may not be adaptable for the screening of inhibitors of dna binding. on the other hand, using a quantitative dna-binding enzyme-linked immunosorbent ... | 2013 | 24022461 |
| oxidized lipids activate autophagy in a jnk-dependent manner by stimulating the endoplasmic reticulum stress response. | excessive production of unsaturated aldehydes from oxidized lipoproteins and membrane lipids is a characteristic feature of cardiovascular disease. our previous studies show that unsaturated lipid peroxidation-derived aldehydes such as 4-hydroxy-trans-2-nonenal (hne) promote autophagy in rat aortic smooth muscle cells (rasmc). in this study, we examined the mechanism by which hne induces autophagy. exposure of rasmc to hne led to the modification of several proteins, most of which were identifie ... | 2013 | 24024137 |
| measurement of hne-protein adducts in human plasma and serum by elisa-comparison of two primary antibodies. | there is increasing evidence that non-enzymatic post-translational protein modifications might play key roles in various diseases. these protein modifications can be caused by free radicals generated during oxidative stress or by their products generated during lipid peroxidation. 4-hydroxynonenal (hne), a major biomarker of oxidative stress and lipid peroxidation, has been recognized as important molecule in pathology as well as in physiology of living organisms. therefore, its detection and qu ... | 2013 | 24024156 |
| microfluidic tool coupled with electrochemical assay for detection of lactoferrin isolated by antibody-modified paramagnetic beads. | lactoferrin (lf) is approximately 80 kda iron-binding protein, which is important part of saliva and other body fluids. due to its ability to bind metal ions, it has many biologically important functions. in this study, a method for the isolation of lf from a biological sample using robotically prepared antibody-modified paramagnetic particles was developed using robotic pipetting station. the method consisted of the following optimised steps. protein g was bound on the paramagnetic particles, o ... | 2013 | 24024242 |
| plasmon-enhanced enzyme-linked immunosorbent assay on large arrays of individual particles made by electron beam lithography. | ultrasensitive biosensing is one of the main driving forces behind the dynamic research field of plasmonics. we have previously demonstrated that the sensitivity of single nanoparticle plasmon spectroscopy can be greatly enhanced by enzymatic amplification of the refractive index footprint of individual protein molecules, so-called plasmon-enhanced enzyme-linked immunosorbent assay (elisa). the technique, which is based on generation of an optically dense precipitate catalyzed by horseradish per ... | 2013 | 24025047 |
| surface-initiated dehydrogenative polymerization of monolignols: a quartz crystal microbalance with dissipation monitoring and atomic force microscopy study. | this work highlights a real-time and label-free method to monitor the dehydrogenative polymerization of monolignols initiated by horseradish peroxidase (hrp) physically immobilized on surfaces using a quartz crystal microbalance with dissipation monitoring (qcm-d). the dehydrogenative polymer (dhp) films are expected to provide good model substrates for studying ligninolytic enzymes. the hrp was adsorbed onto gold or silica surfaces or onto and within porous desulfated nanocrystalline cellulose ... | 2013 | 24032374 |
| hollow graphitic nanocapsules as efficient electrode materials for sensitive hydrogen peroxide detection. | carbon nanomaterials are typically used in electrochemical biosensing applications for their unique properties. we report a hollow graphitic nanocapsule (hgn) utilized as an efficient electrode material for sensitive hydrogen peroxide detection. methylene blue (mb) molecules could be efficiently adsorbed on the hgn surfaces, and this adsorption capability remained very stable under different ph regimes. hgns were used as three-dimensional matrices for coimmobilization of mb electron mediators an ... | 2014 | 24035356 |
| mechanism of protein decarbonylation. | ligand/receptor stimulation of cells promotes protein carbonylation that is followed by the decarbonylation process, which might involve thiol-dependent reduction (c.m. wong et al., circ. res. 102:301-318; 2008). this study further investigated the properties of this protein decarbonylation mechanism. we found that the thiol-mediated reduction of protein carbonyls is dependent on heat-labile biologic components. cysteine and glutathione were efficient substrates for decarbonylation. thiols decre ... | 2013 | 24044890 |
| a new assay for fast, reliable crim status determination in infantile-onset pompe disease. | pompe disease is caused by a deficiency of acid α-glucosidase (gaa; ec, 3.2.1.20), and the infantile-onset form is rapidly fatal if left untreated. however, recombinant human gaa (rhgaa) enzyme replacement therapy (ert) extends survival for infantile pompe patients. although cross-reactive immunologic material (crim)-negative patients, who lack detectable endogenous gaa, mount an immune response to rhgaa that renders the therapy ineffective, timely induction of immune tolerance in these patients ... | 2014 | 24044919 |
| finding the best antibody dilution by repeated immunostaining of the same tissue section. | one can find the optimal antibody dilution for immunostaining by repeated staining on the same tissue section by using a less dilute antibody for each attempt. using secondary antibody and horseradish peroxidase conjugated to a dextran polymer, a section stained repeatedly with several dilutions of antibody appears as good as a section stained with only the last dilution. | 2014 | 24047151 |
| dye coupling and immunostaining of astrocyte-like glia following intracellular injection of fluorochromes in brain slices of the grasshopper, schistocerca gregaria. | injection of fluorochromes such as alexa fluor(®) 568 into single cells in brain slices reveals a network of dye-coupled cells to be associated with the central complex. subsequent immunolabeling shows these cells to be repo positive/glutamine synthetase positive/horseradish peroxidase negative, thus identifying them as astrocyte-like glia. dye coupling fails in the presence of n-heptanol indicating that dye spreads from cell to cell via gap junctions. a cellular network of dye-coupled, astrocyt ... | 2014 | 24048929 |
| sensitive whole-mount fluorescent in situ hybridization in zebrafish using enhanced tyramide signal amplification. | whole-mount in situ hybridization is the preferred method for detecting transcript distributions in whole embryos, tissues, and organs. we present here a sensitive fluorescent in situ hybridization method for colocalization analysis of different transcripts in whole embryonic zebrafish brains. the method is based on simultaneous hybridization of differently hapten-labeled rna probes followed by sequential rounds of horseradish peroxidase (pod)-based transcript detection. sequential detection inv ... | 2014 | 24048934 |
| fast in situ enzymatic gelation of ppo-peo block copolymer for injectable intraocular lens in vivo. | foldable intraocular lenses (iols) have been utilized to substitute natural lens of cataract patients. in this study, we developed a fast, in situ gelable hydrogel requiring no toxic agent as an injectable iol material. a 4-armed ppo/peo-phenol conjugate by a non-degradable linker was synthesized to form a hydrogel in situ by horseradish peroxidase. the gelation time and modulus could be controlled, ranging from 20 s to 2 min and from 1 to 43 kpa. the adhesion of human lens epithelial cells on t ... | 2014 | 24051202 |
| overview of bacterial cellulose composites: a multipurpose advanced material. | bacterial cellulose (bc) has received substantial interest owing to its unique structural features and impressive physico-mechanical properties. bc has a variety of applications in biomedical fields, including use as biomaterial for artificial skin, artificial blood vessels, vascular grafts, scaffolds for tissue engineering, and wound dressing. however, pristine bc lacks certain properties, which limits its applications in various fields; therefore, synthesis of bc composites has been conducted ... | 2013 | 24053844 |
| an assay for pro-oxidant reactivity based on phenoxyl radicals generated by laccase. | a transient species may be detected with uv-vis and epr spectroscopy during turnover of a laccase with quercetin; this species is assigned as a quercetin-derived radical, based on epr spectra as well the observed uv-vis similarities (a 540nm centred band) with previously reported data. the rates of formation and decay of this species correlate well (r=0.9946) with the pro-oxidant reactivity manifested by flavonoids in the presence of laccase. an assay for the pro-oxidant reactivity of natural pr ... | 2014 | 24054233 |
| a synthetic compound, 4-acetyl-3-methyl-6-(3,4,5-trimethoxyphenyl)pyrano[3,4-c]pyran-1,8-dione, ameliorates ovalbumin-induced asthma. | eosinophilia is one of the characteristic signs of allergic inflammation. massive migration of eosinophils to the airways can cause epithelial tissue injury, contraction of airway smooth muscle and increased bronchial responsiveness. previously, we discovered a new compound, 1h,8h-pyrano[3,4-c]pyran-1,8-dione (ppy), derived from the fruit of vitex rotundifolia l. and evaluated its anti-inflammatory and anti-asthmatic properties. in this study, we synthesized a new modified compound, 4-acetyl-3-m ... | 2013 | 24054491 |
| colorimetric and electrochemical genosensors for the detection of escherichia coli dna without amplification in seawater. | monitoring seawater, particularly recreational water, for indicator bacteria presence is required to protect the public from exposure to fecal pollution and to guarantee the safety of the swimming areas. two methods for the detection and quantification of escherichia coli dna were developed: a colorimetric assay in a microplate and an electrochemical biosensor. these assays were based on the double hybridization recognition of a single-strand dna capture probe immobilized onto the microplate or ... | 2013 | 24054570 |
| 3-(10'-phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive elisa of methylglyoxal-modified low density lipoprotein. | using a full factorial design the optimization of experimental conditions of enhanced chemiluminescence reaction (ecr) catalyzed by horseradish peroxidase (hrp) in the presence of 3-(10'-phenothiazinyl)propionic acid (ppa) as a primary enhancer was performed. the effect of concentrations of ppa, hydrogen peroxide, morph, luminol, and tris on a ratio of peroxidase-catalyzed cl to background was studied. the detection limit value of hrp in ecr with ppa was 0.09 pm. using ppa the ultra-sensitive ch ... | 2013 | 24054611 |
| anionic polythiophene derivative as peroxidase mimetics and their application for detection of hydrogen peroxide and glucose. | in this paper, we discovered that the anionic polythiophenes derivative, poly[2-(3-thienyl)ethyloxy-4-butylsulfonate] (ptebs), possesses intrinsic peroxidase-like activity that can catalyze the reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (tmb) in the presence of h2o2 to produce a blue color reaction, which provides colorimetric detection of h2o2. ptebs exhibits several advantages such as high catalytic efficiency, good stability, and rapid response over horseradish peroxidase ... | 2013 | 24054671 |
| differential expression of alpha ii spectrin in monocytes of tuberculosis patients. | monocytes play a crucial role in immune response to tuberculosis. the present study focuses on identifying differences in the monocyte proteome profile of tuberculosis patients, household contacts and healthy controls. differential protein expression was studied by two-dimensional (2d) gel electrophoresis. one of the spots consistently showed either lower intensity or was absent in patients and was identified as alpha ii-spectrin. the decreased expression of αii-spectrin was further validated by ... | 2013 | 24055022 |
| validated detection of anti-gd2 antibody ch14.18/cho in serum of neuroblastoma patients using anti-idiotype antibody ganglidiomab. | human/mouse chimeric monoclonal antibody (mab) ch14.18 is directed against disialoganglioside gd2 and has demonstrated activity and efficacy in high-risk neuroblastoma (nb). for the purpose of industrial production, ch14.18 was manufactured in chinese hamster ovarian cells (ch14.18/cho) in order to facilitate clinical trials in europe. to determine immunopharmacological effects of ch14.18 in preclinical models and clinical trials, a validated method of quantitative detection of ch14.18/cho in se ... | 2013 | 24055592 |
| a paper-based microfluidic electrochemical immunodevice integrated with amplification-by-polymerization for the ultrasensitive multiplexed detection of cancer biomarkers. | a novel signal amplification strategy for ultrasensitive multiplexed detection of cancer biomarkers using a paper-based microfluidic electrochemical immunodevice is described. specifically, a controlled radical polymerization reaction is triggered after the capture of target molecules on the immunodevice surface. growth of long chain polymeric materials provides numerous sites for subsequent horseradish peroxidase (hrp) coupling, which in turn significantly enhances electrochemical signal output ... | 2014 | 24055931 |
| multiplex acute leukemia cytosensing using multifunctional hybrid electrochemical nanoprobes at a hierarchically nanoarchitectured electrode interface. | we have developed a robust, nanobiotechnology-based electrochemical cytosensing approach with high sensitivity, selectivity, and reproducibility toward the simultaneous multiplex detection and classification of both acute myeloid leukemia and acute lymphocytic leukemia cells. the construction of the electrochemical cytosensor involves the hierarchical assembly of dual aptamer-functionalized, multilayered graphene-au nanoparticle electrode interface and the utilization of hybrid electrochemical n ... | 2013 | 24056731 |
| characterization of a layered methylene blue/vanadium oxide nanocomposite and its application in a reagentless h2o2 biosensor. | layered nanocomposite of methylene blue (mb)-intercalated vanadium oxide was obtained through a simple hydrothermal synthesis method using mb, v2o5, and nai as starting materials. the intercalation reaction was proven to be successful using x-ray diffraction pattern. the mb-v2o5 nanocomposite was characterized using a scanning electron micrograph, infrared spectra, thermogravimetric analysis, uv spectra, and electrochemical measurements. the intercalated mb cations showed a fine diffusion-contro ... | 2014 | 24057304 |
| metal-binding ability of vip1: a bzip protein in arabidopsis thaliana. | vire2-interacting protein 1 (vip1) is an arabidopsis thaliana bzip transcription factor which regulates pathogen responses and rehydration responses. vip1 has transcriptional activation potential, dna-binding ability, and a nuclear-cytoplasmic shuttling property. these functions are possibly regulated by cofactors and/or post-translational modifications. during an investigation of the functions of vip1, we discovered that vip1 can react with an ni²⁺-activated derivative of horseradish peroxidase ... | 2013 | 24057918 |
| [the construction and the expression of v5 epitope fused human androgen receptor vector in the yeast cell]. | when we try to establish the gene recombinant yeast cell to screen the androgenic endocrine disruptors, the key procedure is the androgen receptor (ar) expression in the yeast cell. for this purpose, we obtained the gpd (glyceraldehyde-3-phosphote dehydrogenase) promoter from the yeast genosome of w303-1a using pcr system and inserting it into swa i and bamh i sites of pyestrp2. the new constructed vector was named pgpd. the v5 epitope tag dna with a 5'-bamh i and a 3'-ecor i sticky end was clon ... | 2013 | 24059072 |
| amplified colorimetric detection of mercuric ions through autonomous assembly of g-quadruplex dnazyme nanowires. | an amplified colorimetric detection of hg(2+) is proposed by combining t-hg(2+)-t base pairs and hybridization chain reaction (hcr). two hairpins consisting of three-fourths and one-fourth of the horseradish peroxidase (hrp)-mimicking dnazyme in inactive configuration are used as functional elements. in the presence of hg(2+), one of the hairpins is opened by an assistant probe with the help of t-hg(2+)-t base pairs and this triggers an autonomous cross-opening of the two hairpins using the stra ... | 2014 | 24060975 |
| comparison of lignin peroxidase and horseradish peroxidase for catalyzing the removal of nonylphenol from water. | concentrations of aqueous-phase nonylphenol (np), a well-known endocrine-disrupting chemical, are shown to be reduced effectively via reaction with lignin peroxidase (lip) or horseradish peroxidase (hrp) and hydrogen peroxide. we systematically assessed their reaction efficiencies at varying conditions, and the results have confirmed that the catalytic performance of lip toward np was more efficient than that of hrp under experimental conditions. mass spectrum analysis demonstrated that polymeri ... | 2014 | 24062065 |
| [preparation of organic-inorganic hybrid boronate affinity monolith via thiol-ene click reaction for specific capture of glycoproteins]. | a novel strategy for the preparation of the organic-inorganic hybrid boronate affinity monolith was developed via the "thiol-ene" click reaction. a thiol group-modified silica monolith was first synthesized via the sol-gel process by the in situ co-condensation with tetramethoxysilane (tmos) and 3-mercaptopropyltrimethoxysilane (mptms) as precursors. then 3-acrylamidophenylboronic acid (aapba) was covalently immobilized on the hybrid monolith via the "thiol-ene" click reaction to form aapba-sili ... | 2013 | 24063191 |
| [characterization of stability of polypeptides and glycoproteins by capillary electrophoresis]. | a capillary electrophoresis (ce) method was developed for the stability characterization of polypeptides and glycoproteins. angiotensin ii (ang ii), phytagglutinin (pha), bovine thrombin (b-thr), human thrombin (h-thr) and horseradish peroxidase (hrp) were used as polypeptide and glycoprotein mode molecules. the parameters affecting the analysis efficiency for ang ii, such as sample concentration, running buffer, ph and ionic strength of sample solution were optimized, as for the glycoprotein, c ... | 2013 | 24063193 |
| eicosapentaenoic acid enhances heat stress-impaired intestinal epithelial barrier function in caco-2 cells. | dysfunction of the intestinal epithelial tight junction (tj) barrier is known to have an important etiologic role in the pathophysiology of heat stroke. n-3 polyunsaturated fatty acids (pufas), including eicosapentaenoic acid (epa) and docosahexaenoic acid (dha), play a role in maintaining and protecting the tj structure and function. this study is aimed at investigating whether n-3 pufas could alleviate heat stress-induced dysfunction of intestinal tight junction. | 2013 | 24066055 |
| developing new materials for paper-based diagnostics using electrospun nanofibers. | the use of electrospun nanofibers as functional material in paper-based lateral flow assays (lfas) was studied. specific chemical features of the nanofibers were achieved by doping the base polymer, poly(lactic acid) (pla), with poly(ethylene glycol) (peg) and polystyrene8k-block-poly(ethylene-ran-butylene)25k-block-polyisoprene10k-brij76 (k3-brij76) (kb). the lfas were assembled such that the sample flowed through the nanofiber mat via capillary action. initial investigations focused on the sus ... | 2014 | 24068171 |
| quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification for ultrasensitive detection of clenbuterol. | an ultrasensitive electrochemiluminescence (ecl) immunosensor based on cdse quantum dots (qds) has been designed for the detection of clenbuterol. the immunosensor was fabricated by layer by layer and characterized with atomic force microscopic images (afm) and electrochemical impedance spectra (eis). in oxygen-saturated ph=9.0 tris-hcl buffer, a strong ecl emission of qds could be observed during the cathodic process due to the h2o2 product from electrochemical reduction of dissolved oxygen. up ... | 2013 | 24070487 |
| progranulin directly binds to the crd2 and crd3 of tnfr extracellular domains. | we previously reported that pgrn directly bound to tnf receptors (tnfr) in vitro and in chondrocytes (tang, et al., science, 2011). here we report that pgrn also associated with tnfr in splenocytes, and inhibited the binding of tnfα to immune cells. proper folding of pgrn is essential for its binding to tnfr, as dtt treatment abolished its binding to tnfr. in contrast, the binding of pgrn to sortilin was enhanced by dtt. protein interaction assays with mutants of the tnfr extracellular domain de ... | 2013 | 24070898 |
| chitosan-g-hematin: enzyme-mimicking polymeric catalyst for adhesive hydrogels. | phenol derivative-containing adhesive hydrogels has been widely recognized as having potential for biomedical applications, but their conventional production methods, utilizing a moderate/strong base, alkaline buffers, the addition of oxidizing agents or the use of enzymes, require alternative approaches to improve their biocompatibility. in this study, we report a polymeric, enzyme-mimetic biocatalyst, hematin-grafted chitosan (chitosan-g-hem), which results in effective gelation without the us ... | 2014 | 24071001 |
| application of bispecific antibody against antigen and hapten for immunodetection and immunopurification. | we present a bispecific antibody that recognizes an antigen and a hapten and can be applied to various biological assays, including immunoblotting and immunoprecipitation. in immunoblot analysis of serum, an anti-c5 × anti-cotinine bispecific tandem single-chain variable fragment (scfv)-fc fusion protein and cotinine-conjugated horseradish peroxidase (hrp) generated a clean signal without the high background that was observed in a parallel experiment using hrp-conjugated goat anti-rabbit immunog ... | 2013 | 24071736 |
| a fluorescent peroxidase probe increases the sensitivity of commercial elisas by two orders of magnitude. | the low detection sensitivity of enzyme linked immunosorbent assays (elisas) is a central problem in science and limits progress in multiple areas of biology and medicine. in this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ros) probes, can act as turn on fluorescent horseradish peroxidase (hrp) probes and thereby increase the sensitivity of conventional elisas by two orders of magnitude. | 2013 | 24071916 |
| reflux induces dna strand breaks and expression changes of mmp1+9+14 in a human miniorgan culture model. | gastroesophageal reflux disease has been implicated in the pathogenesis of adenocarcinoma of the oesophagus. the same applies to laryngopharyngeal reflux (lpr) and squamous cell cancer of the head and neck, but so far, this link has not been proven. the impact of low ph and bile acids has not been studied extensively in cells other than oesophageal cancer cell lines and tissue. the aims of this study were to investigate the pathogenic potential of reflux and its single components on the mucosa o ... | 2013 | 24075964 |
| changes in synaptic transmission and protein expression in the brains of adult offspring after prenatal inhibition of the kynurenine pathway. | during early brain development, n-methyl-d-aspartate (nmda) receptors are involved in cell migration, neuritogenesis, axon guidance and synapse formation, but the mechanisms which regulate nmda receptor density and function remain unclear. the kynurenine pathway of tryptophan metabolism includes an agonist (quinolinic acid) and an antagonist (kynurenic acid) at nmda receptors and we have previously shown that inhibition of the pathway using the kynurenine-3-monoxygenase inhibitor ro61-8048 in la ... | 2013 | 24076085 |
| epithelial cell adhesion molecule (epcam) is associated with prostate cancer metastasis and chemo/radioresistance via the pi3k/akt/mtor signaling pathway. | prostate cancer (cap) is the second leading malignancy in men. the role of epithelial cell adhesion molecule (epcam), also known as cd326, in cap progression and therapeutic resistance is still uncertain. here, we aimed to investigate the roles of epcam in cap metastasis and chemo/radioresistance. expression of epcam in cap cell lines and human cap tissues was assessed using immunofluorescence and immunohistochemistry, respectively. epcam was knocked down (kd) in pc-3, du145 and lncap-c4-2b cell ... | 2013 | 24076216 |
| involvement of the spinal nalp1 inflammasome in neuropathic pain and aspirin-triggered-15-epi-lipoxin a4 induced analgesia. | neuroinflammation plays an important role in nerve-injury-induced neuropathic pain, but the explicit molecular mechanisms of neuroinflammation in neuropathic pain remain unclear. as one of the most critical inflammatory cytokines, interleukin-1β (il-1β) has been regarded as broadly involved in the pathology of neuropathic pain. the inflammasome caspase-1 platform is one primary mechanism responsible for the maturation of il-1β. lipoxins, a type of endogenous anti-inflammatory lipid, have proved ... | 2013 | 24076348 |
| antimicrobial activity of isothiocyanates (itcs) extracted from horseradish (armoracia rusticana) root against oral microorganisms. | the antimicrobial activity of isothiocyanates (itcs) extracted from horseradish root was investigated against oral microorganisms: 6 strains of facultative anaerobic bacteria, streptococcus mutans, streptococcus sobrinus, lactobacillus casei, staphylococcus aureus, enterococcus faecalis and aggregatibacter actinomycetemcomitans; one strain of yeast, candida albicans, and 3 strains of anaerobic bacteria, fusobacterium nucleatum, prevotella nigrescens, and clostridium perfringens. the itcs extract ... | 2013 | 24077540 |
| on the use of l-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of nadph oxidase: a reevaluation. | l-012, a luminol-based chemiluminescent (cl) probe, is widely used in vitro and in vivo to detect nadph oxidase (nox)-derived superoxide (o2(*-)) and identify nox inhibitors. yet understanding of the free radical chemistry of the l-012 probe is still lacking. we report that peroxidase and h2o2 induce superoxide dismutase (sod)-sensitive, l-012-derived cl in the presence of oxygen. o2(*-) alone does not react with l-012 to emit luminescence. self-generated o2(*-) during oxidation of l-012 and lum ... | 2013 | 24080119 |
| postnatal development of glycine receptor subunits α1, α2, α3, and β immunoreactivity in multiple brain stem respiratory-related nuclear groups of the rat. | the respiratory system is immature at birth and significant development occurs postnatally. a critical period of respiratory development occurs in rats around postnatal days 12-13, when enhanced inhibition dominates over suppressed excitation. the mechanisms underlying the heightened inhibition are not fully understood. the present study tested our hypothesis that the inhibition is marked by a switch in glycine receptor subunits from neonatal to adult form around the critical period. an in-depth ... | 2013 | 24080401 |
| immobilization of horseradish peroxidase on electrospun microfibrous membranes for biodegradation and adsorption of bisphenol a. | horseradish peroxidase (hrp) from roots of horseradish (amoracia rusticana) was successfully immobilized on novel enzyme carriers, poly(methyl methacrylate-co-ethyl acrylate) (pmma cea) microfibrous membranes, and used for removal of bisphenol a from water. pmma cea fibrous membranes (pfm) with fiber diameters of 300-500 nm, were fabricated by electrospinning. hrp was covalently immobilized on the surface of microfibers previously activated by polyethylenimine and glutaraldehyde. hrp loading rea ... | 2013 | 24096278 |
| multidisciplinary approach to study the effect of water status and mobility on the activity of peroxidase in solutions. | the effect of water mobility on horseradish peroxidase (hrp) activity in solutions was investigated by measuring water activity (aw), freezable water content, (1)h proton transverse relaxation time and water self-diffusivity determined by nuclear magnetic resonance. the effect of system mobility as described by viscosity and glass transition temperature (t'g) was also studied. the aw and viscosity of aqueous solutions were modulated using ligands (glucose, sorbitol and trehalose) and a thickener ... | 2014 | 24099539 |
| covalent bond formation between amino acids and lignin: cross-coupling between proteins and lignin. | the present study characterized the products formed from the reaction of amino acids and in turn, proteins, with lignin resulting in cross-coupling. when added to reaction mixtures containing coniferyl alcohol, horseradish peroxidase and h2o2, three amino acids (cys, tyr, and thr) are able to form adducts. the low molecular weight products were analyzed by hplc and from each reaction mixture, one product was isolated and analyzed by lc/ms. lc/ms results are consistent with bond formation between ... | 2013 | 24099658 |
| prevention of hemolysis-induced organ damage by nutritional activation of the vagal anti-inflammatory reflex*. | acute hemolysis is associated with organ damage, inflammation, and impaired vascular function. stimulation of the cholecystokinin-1 receptor-dependent vagal anti-inflammatory reflex with lipid-rich enteral nutrition was demonstrated to prevent tissue damage and attenuate inflammation. this study investigates the effects of nutritional activation of the vagal anti-inflammatory reflex on organ integrity, systemic inflammation, and microcirculation during hemolysis. | 2013 | 24105450 |
| luminescence amplification by enzymatic eu2+ oxidation to eu3+ for time-resolved peroxidase activity measurement. | a novel peroxidase activity assay was developed for horseradish peroxidase (hrp) and myeloperoxidase (mpo), in which substrate eu(2+) was catalytically oxidized to eu(3+), and the eu(3+) luminescence was enhanced by the addition of sensitizer 4,4'-bis(1″,1″,1″,2″,2″,3″,3″-hepatafluoro-4″,6″-hexanedione-6″-yl)chlorosulfo-o-terphenyl (bhhct) for time-resolved measurement of the bhhct-eu(3+) complex. since bhhct-eu(3+) has a long lifetime (more than 500 μs), typical of eu(3+) oxidation state, and t ... | 2013 | 24107562 |
| myosin ii regulates activity dependent compensatory endocytosis at central synapses. | recent evidence suggests that endocytosis, not exocytosis, can be rate limiting for neurotransmitter release at excitatory cns synapses during sustained activity and therefore may be a principal determinant of synaptic fatigue. at low stimulation frequencies, the probability of synaptic release is linked to the probability of synaptic retrieval such that evoked release results in proportional retrieval even for release of single synaptic vesicles. the exact mechanism by which the retrieval rates ... | 2013 | 24107946 |
| selective permeabilization of the blood-brain barrier at sites of metastasis. | effective chemotherapeutics for primary systemic tumors have limited access to brain metastases because of the blood-brain barrier (bbb). the aim of this study was to develop a strategy for specifically permeabilizing the bbb at sites of cerebral metastases. | 2013 | 24108809 |
| phenol removal from refinery wastewater by mutant recombinant horseradish peroxidase. | application of mutated recombinant horseradish peroxidase (hrp) for phenol removal from refinery effluents is reported. recombinant hrp produced in escherichia coli suffers from the disadvantage of lacking glycosylation, which affects its catalytic efficiency and stability toward inactivating parameters such as increased temperature and enhanced amounts of hydrogen peroxide. in the present study, the previously reported variant (in which asn268 was substituted with asp, n268d) with improved stab ... | 2014 | 24112382 |
| an amplex red-based fluorometric and spectrophotometric assay for l-asparaginase using its natural substrate. | we report on the development of a sensitive real-time assay for monitoring the activity of l-asparaginase that hydrolyzes l-asparagine to l-aspartate and ammonia. in this method, l-aspartate is oxidized by l-aspartate oxidase to iminoaspartate and hydrogen peroxide (h2o2), and in the detection step horseradish peroxidase uses h2o2 to convert the colorless, nonfluorescent reagent amplex red to the red-colored and highly fluorescent product resorufin. the assay was validated in both the absorbance ... | 2014 | 24113285 |
| grp78 secreted by tumor cells stimulates differentiation of bone marrow mesenchymal stem cells to cancer-associated fibroblasts. | cancer-associated fibroblasts (cafs), one type of tumor-associated stromal cells, have been shown to provide a favorable environment for the malignant tumor progression. extensive reports have demonstrated that mesenchymal stem cells (mscs) can function as precursors for cafs. however, the mechanisms by which tumor cells induce the transition of mscs to cafs have not been well established. grp78, traditionally known as an endoplasmic reticulum (er) chaperone, has been identified to overexpress i ... | 2013 | 24113381 |
| triple signal amplification using gold nanoparticles, bienzyme and platinum nanoparticles functionalized graphene as enhancers for simultaneous multiple electrochemical immunoassay. | here we demonstrated an ultrasensitive electrochemical immunoassay employing graphene, platinum nanoparticles (ptnps), glucose oxidase (god) and horseradish peroxidase (hrp) as enhancers to simultaneously detect carcinoembryonic antigen (cea) and alpha-fetoprotein (afp). this immunosensor is based on the observation that multiple-labeled antibodies (thionine-labeled anti-cea and ferrocene-labeled anti-afp) recognition event yielded a distinct voltammetric peak through "sandwich" immunoreaction, ... | 2014 | 24113435 |
| laminated paper-based analytical devices (lpad) with origami-enabled chemiluminescence immunoassay for cotinine detection in mouse serum. | laminated paper-based analytical devices (lpad) with origami-enabled chemiluminescence immunoassay have been developed for the detection of cotinine, a secondhand smoke (shs) biomarker. the devices were fabricated by a craft-cutter to define flow channels, followed by lamination. this approach of cutting/lamination to fabricate lpad is very similar to making an identification card, offering advantages in simplicity and rugged backing when compared to the common method of patterning paper using s ... | 2013 | 24117197 |
| the role of survivin in thyroid tumors: differences of expression in well-differentiated, non-well-differentiated, and anaplastic thyroid cancers. | survivin is involved in human cancer and is responsible for aggressive biological behavior and poor clinical outcomes in several human malignancies. thus, we hypothesized that the upregulation of survivin protein expression may be enhanced in parallel with transition toward a poorly differentiated phenotype in human thyroid carcinomas. | 2014 | 24117205 |
| estrogen reduces endoplasmic reticulum stress to protect against glucotoxicity induced-pancreatic β-cell death. | estrogen can improve glucose homeostasis not only in diabetic rodents but also in humans. however, the molecular mechanism by which estrogen prevents pancreatic β-cell death remains unclear. to investigate this issue, ins-1 cells, a rat insulinoma cell line, were cultured in medium with either 11.1mm or 40mm glucose in the presence or the absence of estrogen. estrogen significantly reduced apoptotic β-cell death by decreasing nitrogen-induced oxidative stress and the expression of the er stress ... | 2014 | 24120916 |
| construction of highly ordered polyaniline nanowires and their applications in dna sensing. | a novel electrochemical active polyaniline (pani) nanowire was fabricated and utilized for the construction of a highly sensitive and selective electrochemical sensor for hepatitis b virus gene. the uniform pani nanowire was prepared by the enzymatic polymerization of aniline monomers on the amyloid-like nanofiber (ap nanowire), which was self-assembled from an aniline-attached nonapeptide, aniline-ggaaklvff (ap). the prepared pani nanowires were characterized by electron microscopy, uv-vis abso ... | 2014 | 24121157 |
| vegf111b, a new member of vegfxxxb isoforms and induced by mitomycin c, inhibits angiogenesis. | vascular endothelial growth factor (vegf-a) stimulating angiogenesis is required for tumor growth and progression. the conventional vegf-a isoforms have been considered as pro-angiogenic factors. another family of vegf-a isoforms generated by alternative splicing, termed vegfxxxb isoforms, has anti-angiogenic property, exemplified by vegf165b. here, we identify a new number of vegfxxx family-vegf111b induced by mitomycin c, although not detected in mitomycin c-unexposed ovarian cancer cells. sko ... | 2013 | 24125722 |
| seasonal lectin binding variations of thumb pad in the frog (pelophylax ridibundus). | the thumb pad is one of the most common secondary sexual characteristics in frogs. although it is known that amphibian skin has affinity for several lectins, there is no report regarding lectin-binding affinity of the thumb pad or its structural components. this study investigated localization and seasonal variation of specific carbohydrate moieties of glycoconjugates in both the epidermal and dermal components of the frog thumb pad at the light microscopic level using lectin histochemistry. the ... | 2014 | 24127244 |
| in vivo modulation of polo-like kinases supports a key role for plk2 in ser129 α-synuclein phosphorylation in mouse brain. | α-synuclein is the major component of lewy bodies. α-synuclein phosphorylated at ser 129 (phospho-α-syn) is the most common synuclein modification observed in parkinson's disease pathology and transgenic animal models. polo-like kinase 2 (plk2) was previously proposed as an important kinase in α-synuclein phosphorylation at ser129. to better understand the role of plk2 in α-synuclein phosphorylation in vivo, we further evaluated the effect of plk2 genetic knockdown and pharmacological inhibition ... | 2014 | 24128992 |
| regeneration of optic nerve fibers with unoprostone, a prostaglandin-related antiglaucoma drug, in adult cats. | we investigated the effects of unoprostone on neurite extension of cultured retinal pieces and axonal regeneration of retinal ganglion cells in the crushed optic nerve of adult cats. | 2014 | 24129676 |
| high glucose enhances lps-stimulated human pmvec hyperpermeability via the no pathway. | chronic hyperglycemia is an established risk factor for endothelial damage. it remains unclear, however, whether brief hyperglycemic exposure exacerbates the damage to vascular endothelial cells induced by endotoxin. we hypothesize that brief hyperglycemic exposure enhances the permeability of the endothelium following stimulation with lipopolysaccharide (lps). correlations between modulation of nitric oxide synthase (nos) pathways and altered endothelial homeostasis have been studied and demons ... | 2013 | 24137189 |
| correlations between blood-brain barrier disruption and neuroinflammation in an experimental model of penetrating ballistic-like brain injury. | abstract blood-brain barrier (bbb) disruption is a pathological hallmark of severe traumatic brain injury (tbi) and is associated with neuroinflammatory events contributing to brain edema and cell death. the goal of this study was to elucidate the profile of bbb disruption after penetrating ballistic-like brain injury (pbbi) in conjunction with changes in neuroinflammatory markers. brain uptake of biotin-dextran amine (bda; 3 kda) and horseradish peroxidase (hrp; 44 kda) was evaluated in rats at ... | 2014 | 24138024 |
| integrating bipolar electrochemistry and electrochemiluminescence imaging with microdroplets for chemical analysis. | here we develop a microdroplet sensor based on bipolar electrochemistry and electrochemiluminescence (ecl) imaging. the sensor was constructed with a closed bipolar cell on a hybrid poly(dimethylsioxane) (pdms)-indium tin oxide (ito) glass microchip. the ito microband functions as the bipolar electrode and its two poles are placed in two spatially separate micro-reservoirs predrilled on the pdms cover. after loading microliter-sized liquid droplets of tris(2,2'-bipyridyl) ruthenium (ii)/2-(dibut ... | 2014 | 24140829 |
| target-induced self-assembly of dna nanomachine on magnetic particle for multi-amplified biosensing of nucleic acid, protein, and cancer cell. | a biosensing system is established for the multi-amplified detection of dna or specific substrates of aptamers under isothermal conditions, which combines nicked rolling circle amplification (n-rca) and beacon assisted amplification (baa) with sensitive colorimetric technique by using dnazymes as reporter units. according to the configuration, the analysis of dna is accomplished by recognizing the target to capture nucleic acid-functionalized magnetic particles, followed by the self-assembly of ... | 2014 | 24140870 |
| selective conjugation of proteins by mining active proteomes through click-functionalized magnetic nanoparticles. | superparamagnetic iron oxide nanoparticles (spions) coated with azide groups were functionalized at the surface with biotin (biotin@spions) and cysteine protease inhibitor e-64 (e-64@spions) with the purpose of developing nanoparticle-based assays for identifying cysteine proteases in proteomes. magnetite particles (ca. 6 nm) were synthesized by microwave-assisted thermal decomposition of iron acetylacetonate and subsequently functionalized following a click chemistry protocol to obtain biotin a ... | 2013 | 24143894 |
| determination of okadaic acid in shellfish by using a novel chemiluminescent enzyme-linked immunosorbent assay method. | a direct competitive chemiluminescent enzyme-linked immunosorbent assay (cl-elisa) was developed to determine okadaic acid (oa). concentrations of the capture monoclonal anti-oa antibodies, conjugate of oa-hrp and a composition of blocking buffers were varied to optimize the assay condition. the values of ic10, ic50 and working range (ic20-ic80) for cl-elisa were 0.01, 0.07, and 0.03-0.2 ng/ml, respectively. additionally, the analytical recovery values of cl-elisa from 3 shellfish spiked samples ... | 2013 | 24148413 |
| role of triton x-100 in chemiluminescent enzyme immunoassays capable of diagnosing genetic disorders. | the use of triton x surfactants in developing 1,1'-oxalylimidazole chemiluminescent enzyme immunoassays (odi ceis) with extended linear response range for the quantification of unconjugated estriol (ue3), alpha-fetoprotein (afp), and human chorionic gonadotropin (hcg) is reported for the first time. the wider linear dynamic range in odi cleia results from triton x series (e.g., triton x-100, -114, -405, -705) acting as an inhibitor in the interaction between amplex red (hydrophobic substrate) an ... | 2013 | 24148422 |
| rapid high-throughput analysis of ochratoxin a by the self-assembly of dnazyme-aptamer conjugates in wine. | we report a new label-free colorimetric aptasensor based on dnazyme-aptamer conjugate for rapid and high-throughput detection of ochratoxin a (ota, a possible human carcinogen, group 2b) in wine. two oligonucleotides were designed for this detection. one is n1 for biorecognition, which includes two adjacent sequences: the ota-specific aptamer sequence and the horseradish peroxidase (hrp)-mimicking dnazyme sequence. the other is a blocking dna (b2), which is partially complementary to a part of t ... | 2013 | 24148439 |
| electrochemical immunoassay for carcinoembryonic antigen using gold nanoparticle-graphene composite modified glassy carbon electrode. | carcinoembryonic antigen (cea), which is typically associated with certain tumors and developing fetus, is widely used as a clinical tumor marker for some familiar cancers. in this work, a simple and sensitive electrochemical cea sensor was developed by employing immunoreaction. gold nanoparticle-decorated graphene composites (au-gn) were successfully synthesized based on the reduction of haucl4 in the presence of graphene. horseradish peroxidase-labeled anti-cea antibody (hrp-anti-cea) and hrp ... | 2013 | 24148478 |
| an ultrasensitive chemiluminescent immunosensor for the detection of human leptin using hemin/g-quadruplex dnazymes-assembled signal amplifier. | in this work, we reported a sensitive chemiluminescent immunosensor for the detection of human leptin by using hemin/g-quadruplex dnazymes to amplify detection signal. in this sensing system, the primary antibody (anti-human leptin) was firstly bound to the 96-well plates, and human leptin and biotinylated secondary antibody were successively combined to form sandwich-type immune complex through specific interactions. then streptavidin labeled with hemin/g-quadruplex dnazymes was assembled to th ... | 2013 | 24148479 |
| injectable 3d hydrogel scaffold with tailorable porosity post-implantation. | since rates of tissue growth vary significantly between tissue types, and also between individuals due to differences in age, dietary intake, and lifestyle-related factors, engineering a scaffold system that is appropriate for personalized tissue engineering remains a significant challenge. in this study, a gelatin-hydroxyphenylpropionic acid/carboxylmethylcellulose-tyramine (gtn-hpa/cmc-tyr) porous hydrogel system that allows the pore structure of scaffolds to be altered in vivo after implantat ... | 2014 | 24151286 |
| intracellular nogo-a facilitates initiation of neurite formation in mouse midbrain neurons in vitro. | nogo-a is a transmembrane protein originally discovered in myelin, produced by postnatal cns oligodendrocytes. nogo-a induces growth cone collapse and inhibition of axonal growth in the injured adult cns. in the intact cns, nogo-a functions as a negative regulator of growth and plasticity. nogo-a is also expressed by certain neurons. neuronal nogo-a depresses long-term potentiation in the hippocampus and modulates neurite adhesion and fasciculation during development in mice. here we show that n ... | 2014 | 24157929 |
| plasmodium falciparum ruvb2 translocates in 5'-3' direction, relocalizes during schizont stage and its enzymatic activities are up regulated by ruvb3 of the same complex. | two similar proteins ruvb like1 (rvb1/pontin) and ruvb like2 (rvb2/reptin) of aaa+ family of enzymes are present in yeast to human and are well known to be involved in diverse cellular activities. the human malaria parasite plasmodium falciparum contains three different ruvb like proteins. thus it has been of interest to explore why p. falciparum requires three ruvb like proteins and how these enzymes are biochemically regulated. in this study, we present the detailed biochemical characterizatio ... | 2013 | 24161468 |
| effects of intestinal bacteria-derived p-cresyl sulfate on th1-type immune response in vivo and in vitro. | protein fermentation by intestinal bacteria generates various compounds that are not synthesized by their hosts. an example is p-cresol, which is produced from tyrosine. patients with chronic kidney disease (ckd) accumulate high concentrations of intestinal bacteria-derived p-cresyl sulfate (pcs), which is the major metabolite of p-cresol, in their blood, and this accumulation contributes to certain ckd-associated disorders. immune dysfunction is a ckd-associated disorder that frequently contrib ... | 2014 | 24161588 |
| temperature-controlled encapsulation and release of an active enzyme in the cavity of a self-assembled dna nanocage. | we demonstrate temperature-controlled encapsulation and release of the enzyme horseradish peroxidase using a preassembled and covalently closed three-dimensional dna cage structure as a controllable encapsulation device. the utilized cage structure was covalently closed and composed of 12 double-stranded b-dna helices that constituted the edges of the structure. the double stranded helices were interrupted by short single-stranded thymidine linkers constituting the cage corners except for one, w ... | 2013 | 24168393 |
| celiac disease serum markers and recurrent pregnancy loss. | celiac disease has been associated with numerous unfavorable health outcomes, including pregnancy complications such as infertility, preterm birth, and preeclampsia. however, the association between celiac disease and recurrent pregnancy loss (rpl) remains uncertain. our purpose was to compare serum markers of celiac disease in women with and without rpl. therefore, we performed a case-control study of 116 women with unexplained recurrent pregnancy loss and 116 age-matched controls. maternal ser ... | 2013 | 24176553 |
| efficient sirna delivery and tumor accumulation mediated by ionically cross-linked folic acid-poly(ethylene glycol)-chitosan oligosaccharide lactate nanoparticles: for the potential targeted ovarian cancer gene therapy. | for effective ovarian cancer gene therapy, systemic administrated tumor-targeting sirna/folic acid-poly(ethylene glycol)-chitosan oligosaccharide lactate (fa-peg-col) nanoparticles is vital for delivery to cancer site(s). sirna/fa-peg-col nanoparticles were prepared by ionic gelation for effective fa receptor-expressing ovarian cancer cells transfection and in vivo accumulation. the chemical structure of fa-peg-col conjugate was characterized by maldi-tof-ms, ft-ir and (1)h nmr. the average size ... | 2014 | 24178005 |
| analysis of binding of biotinylated protoplast-release-inducing protein that induces release of gametic protoplasts in the closterium peracerosum-strigosum-littorale complex. | a protoplast-release-inducing protein (pr-ip) which is released from mating-type plus (mt(+)) cells and induces the release of gametic protoplasts from matingtype minus (mt(-)) cells of closterium was biotinylated and then used to examine the interaction of this protein with mt(-) cells. the protoplast-release-inducing activity of pr-ip was not altered after the biotinylation. when mt(-) cells that had been pre-cultured for 24 h were incubated with biotinylated pr-ip for 6 h in nitrogen-deficien ... | 1993 | 24178507 |
| retinal ganglion cells in the pacific redfin, tribolodon brandtii dybowski, 1872: morphology and diversity. | we studied the morphology and diversity of retinal ganglion cells in the pacific redfin, tribolodon brandtii. these cells were retrogradely labeled with horseradish peroxidase and examined in retinal whole mounts. a sample of 203 cells was drawn with a camera lucida. a total of 19 structural parameters were estimated for each cell, and a variety of clustering algorithms were used to classify the cells. the optimal solution was determined by using silhouette analysis. it was based on three variab ... | 2014 | 24178784 |
| quantification of cytochrome 2e1 in human liver microsomes using a validated indirect elisa. | cyp2e1 is an important cytochrome p450 isoform in many endogenous processes and in the metabolism of organic solvents, a number of drugs and pre-carcinogens. information on the abundance of the enzyme may be valuable in various types of research in the field of toxicology and pharmacology. an indirect elisa for the quantification of cyp2e1 in human liver microsomes was developed and successfully validated. all samples, including validation samples and calibrators, were diluted to a final concent ... | 2014 | 24184659 |
| mechanisms of enhanced neutralization of botulinum neurotoxin by monoclonal antibodies conjugated to antibodies specific for the erythrocyte complement receptor. | immune complexes formed between monoclonal antibodies (mabs) and toxins can neutralize toxicity in vivo by multiple mechanisms. toxin sequestration and clearance by mabs may be improved by enhancing their ability to bind to red blood cells (rbcs) through immune adherence. this can be achieved by converting the mabs to heteropolymers (hps), which are antigen-specific mabs cross-linked to mabs targeting the complement receptor (cr1), a protein that is expressed on the surface of rbcs in primates a ... | 2014 | 24184879 |
| horseradish peroxidase inactivation: heme destruction and influence of polyethylene glycol. | horseradish peroxidase (hrp) mediates efficient conversion of many phenolic contaminants and thus has potential applications for pollution control. such potentially important applications suffer however from the fact that the enzyme becomes quickly inactivated during phenol oxidation and polymerization. the work here provides the first experimental data of heme consumption and iron releases to support the hypothesis that hrp is inactivated by heme destruction. product of heme destruction is iden ... | 2013 | 24185130 |
| a novel life cycle arising from leaf segments in plants regenerated from horseradish hairy roots. | horseradish (armoracia rusticana) hairy root clones were established from hairy roots which were transformed with the ri plasmid in agrobacterium rhizogenes 15834. the transformed plants, which were regenerated from hairy root clones, had thicker roots with extensive lateral branches and thicker stems, and grew faster compared with non-transformed horseradish plants. small sections of leaves of the transformed plants generated adventitious roots in phytohormone-free g (modified gamborg's) medium ... | 1995 | 24185335 |
| multi-enzyme co-embedded organic-inorganic hybrid nanoflowers: synthesis and application as a colorimetric sensor. | this study reports a facile method for the synthesis of multi-enzyme co-embedded organic-inorganic hybrid nanoflowers, using glucose oxidase (gox) and horseradish peroxidase (hrp) as the organic components, and cu3(po4)2 · 3h2o as the inorganic component. the synthesized nanoflowers enable the combination of a two-enzyme cascade reaction in one step, in which the gox component of the nanoflowers oxidizes glucose to generate h2o2, which then reacts with the adjacent hrp component on the nanoflowe ... | 2014 | 24186239 |
| the oxidation of oxytocin and vasopressin by peroxidase/h2o 2 system. | oxytocin and vasopressin are oxidized by horseradish peroxidase and by lactoperoxidase, in the presence of hydrogen peroxide. spectrophotometric measurements are indicative of the formation of dityrosine. kinetic parameters indicate that the affinity of horseradish peroxidase is slightly higher for oxytocin with respect to vasopressin and that the two hormones are better substrates for both peroxidases than free tyrosine. | 1995 | 24186516 |
| novel electrochemical aptamer biosensor based on an enzyme-gold nanoparticle dual label for the ultrasensitive detection of epithelial tumour marker muc1. | a novel platform based on a hairpin oligonucleotide (ho) switch, gold nanoparticles (aunps), and enzyme signal amplification for the ultrasensitive detection of mucin 1 protein (muc1) was developed in this assay. this ho aptamers and horseradish peroxidase (hrp) were immobilised on the aunps to yield ho-aunp-hrp conjugates. aunps were used as labels and bridges between the ho and hrp. hrp was also used as label for catalysing the oxidation of o-phenylenediamine by h2o2. the reaction product was ... | 2014 | 24189297 |
| scavenging of free-radical metabolites of aniline xenobiotics and drugs by amino acid derivatives: toxicological implications of radical-transfer reactions. | we investigated a novel scavenging mechanism of arylamine free radicals by poly- and monoaminocarboxylates. free radicals of arylamine xenobiotics and drugs did not react with oxygen in peroxidase-catalyzed reactions; however, they showed marked oxygen uptake in the presence of an aminocarboxylate. these free-radical intermediates were identified using the spin trap 5,5-dimethyl-1-pyrroline-n-oxide (dmpo) and electron paramagnetic resonance (epr) spectrometry. diethylenetriaminepentaacetic acid ... | 2013 | 24191655 |
| preparation of boronate-functionalized molecularly imprinted monolithic column with polydopamine coating for glycoprotein recognition and enrichment. | a novel imprinting strategy using reversible covalent complexation of glycoprotein was described for creating glycoprotein-specific recognition cavities on boronate-functionalized monolithic column. based on it, a molecularly imprinted monolithic column was prepared by self-polymerization of dopamine (da) on the surface of 4-vinylphenylboronic acid (vpba)-based polymeric skeletons after reversible immobilization of horseradish peroxidase (hrp). due to the combination of boronate affinity and sur ... | 2013 | 24192150 |
| uptake and accumulation of ajmalicine into isolated vacuoles of cultured cells of catharanthus roseus (l.) g. don. and its conversion into serpentine. | isolated vacuoles from ajmalicine-producing cell suspensions of catharanthus roseus accumulated the alkaloid ajmalicine. dissipation of the transtonoplast ph gradient with nigericin abolished ajmalicine accumulation, whereas dissipation of the transtonoplast potential with valinomycin had no effect. addition of mg-atp resulted in a higher ajmalicine accumulation. serpentine produced by the cells was largely recovered in isolated vacuoles; in contrast, ajmalicine was lost. ajmalicine was converte ... | 1991 | 24193617 |
| wound-induced expression of horseradish peroxidase. | peroxidases have been implicated in the responses of plants to physiological stress and to pathogens. wound-induced peroxidase of horseradish (armoracia rusticana) was studied. total peroxidase activity was increased by wounding in cell wall fractions extracted from roots, stems and leaves of horseradish. on the other hand, wounding decreased the peroxidase activity in the soluble fraction from roots. the enzyme activities of the basic isozymes were induced by wounding in horseradish leaves base ... | 1994 | 24193641 |
| tunicamycin-inhibited carrot somatic embryogenesis can be restored by secreted cationic peroxidase isoenzymes. | somatic embryogenesis of carrot (daucus carota l.) is inhibited by the glycosylation inhibitor tunicamycin. this inhibition is reversible by the addition of correctly glycosylated glycoproteins which have been secreted into the culture medium. to identify the proteins responsible for complementation, glycoproteins present in the medium of embryo cultures were purified and tested for their activity in the tunicamycin inhibition/ complementation assay. a 38-kda glycoprotein was purified that could ... | 1991 | 24194238 |
| effects of light and growth regulators on adventitious bud formation in horseradish (armoracia rusticana). | to clarify that the presence of ri t-dna genes are not prerequisite for the light-induced bud formation in horseradish (armoracia rusticana) hairy roots, leaf and root segments of nontransformed horseradish plants were used as explants. bud formation from nontransformed tissues was observed in hormone-free medium under 16 h daylight conditions, but not under continuous darkness. to investigate the effects of growth regulators on bud formation, leaf and root explants were treated with auxin (1-na ... | 1995 | 24194306 |
| a novel and efficient polymerization of lignosulfonates by horseradish peroxidase/h(2)o(2) incubation. | lignosulfonates(lss), by-products from chemical pulping processes, are low-value products with limited dispersion properties. the ability of commercially available horseradish peroxidase (hrp) to polymerize ls macromolecules and improve the dispersion properties of lss was investigated. the polymerization of lss proceeded efficiently under mild reaction conditions in an aqueous solution with hrp/h2o2. gel permeation chromatography showed a significant increase in weight-average molecular weight ... | 2013 | 24196582 |