Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| analysis of genetic variations across regulatory and coding regions of kappa-casein gene of indian native cattle (bos indicus) and buffalo (bubalus bubalis). | the promoter region of kappa-casein (κ-cn) gene in indian native cattle and buffalo breeds was sequenced and analyzed for nucleotide variations. sequence comparison across breeds of indian cattle revealed a total of 7 variations in the promoter region, of which - 515 g/t, - 427 c/t, - 385 c/t, - 283 a/g and - 251 c/t were located within consensus binding sites for octamer-binding protein (oct1)/pregnancy specific mammary nuclear factor (pmf), activator protein-2 (ap2), hepatocyte nuclear factor ... | 2014 | 25606460 |
| serum concentrations of haptoglobin and serum amyloid a in water buffaloes (bubalus bubalis) with abomasal ulcer. | to evaluate the serum concentrations of haptoglobin (hp) and serum amyloid a (saa) in water buffaloes with abomasal ulcers, the abomasums of 100 randomly selected water buffaloes were examined after slaughter. type i abomasal ulcers were found in 56 out of 100 buffaloes. serum concentrations of hp and saa were measured. there was no significant difference between affected and non-affected buffaloes in the serum concentrations of hp and saa. the serum concentrations of hp and saa had no significa ... | 2012 | 25610571 |
| comparison digestibility and protozoa population of khuzestan water buffalo and holstein cow. | the major aim of this study was to compare the morphology and activity of rumen protozoa of khuzestan water buffalo and holstein cow using in vitro digestibility and gas production parameters of steam treated sugarcane pith. rumen fluid obtained from two buffalo and cow steers fed the same diet, 30:70 concentrate: forage. to separate rumen protozoa, antibiotic solution and fungicides were added to rumen fluid. the results of present experiment indicated that the neutral detergent fiber (ndf; 7.8 ... | 0 | 25610581 |
| natural babesia bovis infection in water buffaloes (bubalus bubalis) and crossbred cattle under field conditions in egypt: a preliminary study. | there is a little or no data available on the natural babesia bovis (b. bovis) infection in water buffaloes (bubalus bubalis) comparing to the available one for cattle. this study was conducted to investigate the natural b. bovis infection in water buffaloes in comparison to crossbred cattle under field conditions in egypt. | 2013 | 25629060 |
| correction: toll-like receptor responses to peste des petits ruminants virus in goats and water buffalo. | 2015 | 25634030 | |
| high prevalence of schistosoma japonicum and fasciola gigantica in bovines from northern samar, the philippines. | the cause of zoonotic schistosomiasis in the philippines is schistosoma japonicum, which infects up to 46 mammalian hosts, including humans and bovines. in china, water buffaloes have been identified as major reservoir hosts for schistosomiasis japonica, contributing up to 75% of human transmission. in the philippines, water buffaloes (carabao; bubalus bubalis carabanesis) have, historically, been considered unimportant reservoirs. we therefore revisited the possible role of bovines in schistoso ... | 2015 | 25643317 |
| novel sequence types of chlamydia pecorum infect free-ranging alpine ibex (capra ibex) and red deer (cervus elaphus) in switzerland. | chlamydia pecorum, a recognized pathogen of domesticated ruminants and koalas (phascolarctos cinereus), has been recently reported in a broad range of other wildlife species including water buffalo (bubalus bubalis), ibex (capra ibex), chamois (rupicapra rupicapra), red deer (cervus elaphus), and birds. this identification raises questions as to whether cross-host transmission may be a factor in the epidemiology of infections in these species. to begin to address this question, we employed a c. ... | 2015 | 25647593 |
| escherichia coli o157:h7/nm prevalence in raw beef, camel, sheep, goat, and water buffalo meat in fars and khuzestan provinces, iran. | enterohemorrhagic escherichia coli (ehec) of the o157:h7 serotype is a worldwide zoonotic pathogen responsible for the majority of severe cases of human ehec disease. the aim of the present study was to investigate the prevalence of e. coli o157: h7/nm in raw meat samples from two provinces of iran. during a period from march 2010 to march 2011. two hundred and ninety five raw meat samples were collected from beef (n= 85), camel, (n= 50), sheep (n= 62), goat (n= 60), and water buffalo (n=38). fo ... | 2012 | 25653740 |
| defense cells profile of cervical mucous during follicular and luteal phases of estrus cycle in river buffalo. | the aim of this study was to evaluate the defense cells changes of cervical mucous during follicular and luteal phases of estrus cycle in river buffalo. reproductive organs of the adult and apparently healthy female buffaloes were collected from the slaughterhouse. by visual investigation of both the ovaries for presence of corpus luteum and growing follicles, the luteal and follicular phase of each buffalo was specified. cervical discharge samples were collected by sterile swabs and then spread ... | 2012 | 25653745 |
| effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (bubalus bubalis). | the aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (bubalus bubalis) bulls. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 µg ml(-1) sodium selenite and the sperm motility and viability were evaluated at 0 (t0) (immediately after dilution), 60 (t1) and 120 (t2) min after dilu ... | 2012 | 25653769 |
| a review of recent developments in buffalo reproduction - a review. | the buffalo is an important livestock resource in several countries of south asia and the mediterranean regions. however, reproductive efficiency is compromised due to known problems of biological and management origins, such as lack of animal selection and poor nutrition. under optimal conditions puberty is attained at 15 to 18 months in river buffalo, 21 to 24 months in swamp buffalo and is influenced by genotype, nutrition, management and climate. however, under field conditions these values ... | 2015 | 25656203 |
| phenotypic characterization and multivariate analysis to explain body conformation in lesser known buffalo (bubalus bubalis) from north india. | phenotypic characterization and body biometric in 13 traits (height at withers, body length, chest girth, paunch girth, ear length, tail length, length of tail up to switch, face length, face width, horn length, circumference of horn at base, distances between pin bone and hip bone) were recorded in 233 adult gojri buffaloes from punjab and himachal pradesh states of india. traits were analysed by using varimax rotated principal component analysis (pca) with kaiser normalization to explain body ... | 2015 | 25656215 |
| dige based proteome analysis of mammary gland tissue in water buffalo (bubalus bubalis): lactating vis-a-vis heifer. | mammary gland is an exocrine and sebaceous gland made up of branching network of ducts that end in alveoli. milk is synthesized in the alveoli and secreted into alveolar lumen. mammary gland represents an ideal system for the study of organogenesis that undergoes successive cycles of pregnancy, lactation and involution. to gain insights on the molecular events that take place in pubertal and lactating mammary gland, we have identified 43 differentially expressed proteins in mammary tissue of hei ... | 2015 | 25661041 |
| potential functional gene diversity involved in methanogenesis and methanogenic community structure in indian buffalo (bubalus bubalis) rumen. | understanding the methanogen community structure and methanogenesis from bubalus bubalis in india may be beneficial to methane mitigation. our current understanding of the microbial processes leading to methane production is incomplete, and further advancement in the knowledge of methanogenesis pathways would provide means to manipulate its emission in the future. in the present study, we evaluated the methanogenic community structure in the rumen as well as their potential genes involved in met ... | 2015 | 25663664 |
| isolation and functional characterization of buffalo (bubalus bubalis) β-casein promoter for driving mammary epithelial cell-specific gene expression. | therapeutic proteins are produced in microbes, mammalian cell lines, and body fluids by applying recombinant dna technology. they are required for compensating the deficiency of essential proteins in patients. animal bioreactors producing such valuable bio-pharmaceuticals in body fluids have lately emerged as efficient and cost-effective expression systems. promoters, along with other regulatory elements of genes coding for milk proteins, have been cloned from few species for directing the expre ... | 2015 | 25678138 |
| polymorphisms in oxytocin and α1a adrenergic receptor genes and their effects on production traits in dairy buffaloes. | the use of molecular markers may auxiliary the buffalo breeding. the oxytocin (oxt) and the adrenergic receptor α1a (adra1a) may be involved in milk ejection in ruminants. the aim of this study was to verify the existence of polymorphisms in the oxt and adra1a genes and their associations with milk production traits. a total of 220 buffaloes were genotyped using pcr-rflp for both genes. the snp identified in the adra1a gene was associated with protein percentage in dairy buffaloes. this is the f ... | 2015 | 25695522 |
| improving smallholder food security through investigations of carcass composition and beef marketing of buffalo and cattle in northern lao pdr. | this study determined the carcass composition of lao indigenous buffalo (bubalus bubalis) and cattle (bos indicus), then examined trends in bovine meat marketing following review of records of beef production and prices in the two major cities of luang prabang (lpb) and xieng khoung (xk) provinces in northern laos. samples from 41 buffalo and 81 cattle (n = 122) were collected from animals slaughtered in may-june 2014, with live weights, carcass weights and other carcass-related variables collec ... | 2015 | 25700673 |
| comparative expression analysis of gametogenesis-associated genes in foetal and adult bubaline (bubalus bubalis) ovaries and testes. | this study was conducted to identify and analyse the expression of gametogenesis-associated genes and proteins in foetal and adult buffalo gonads of both the sexes. relative quantification of the genes was determined by qpcr and western blotting. immunohistochemistry was also performed for various gametogenesis-associated proteins in foetal and adult gonads of both the sexes. we observed significantly (p < 0.05) increased expression of primordial germ cell-specific, meiotic as well as genes asso ... | 2015 | 25703697 |
| screening diagnostic candidates for schistosomiasis from tegument proteins of adult schistosoma japonicum using an immunoproteomic approach. | schistosomiasis is one of the world's most prevalent zoonotic diseases and a serious worldwide public health problem. since the tegument (tg) of schistosoma japonicum is in direct contact with the host and induces a host immune response against infection, the identification of immune response target molecules in the schistosome tg is crucial for screening diagnostic antigens for this disease. | 2015 | 25706299 |
| molecular epidemiology and phylogenetic analysis of diverse bovine astroviruses associated with diarrhea in cattle and water buffalo calves in china. | astroviruses are the principal causative agents of gastroenteritis in humans and have been associated with diarrhea in other mammals as well as birds. however, astroviral infection of animals had been poorly studied. in the present study, 211 rectal swabs collected from cattle and water buffalo calves with mild to severe diarrhea were tested for bovine astrovirus (bastv) by rt-pcr. results: 92/211 (43.6%) samples were positive for bastv, at a rate of 46.10% (71/154) in cattle and 36.84% (21/57) ... | 2015 | 25716289 |
| short communication: variable number of tandem repeat polymorphisms in dgat1 gene of buffaloes (bubalus bubalis) is associated with milk constituents. | the diacylglycerol-o-transferase 1 gene is a positional and functional candidate for milk composition traits. the objective of this study was to evaluate the segregation of the variable number of tandem repeat polymorphisms in the regulatory region of diacylglycerol-o-transferase 1 gene in a water buffalo herd, and to assess the association of this mutation with milk production traits. for this purpose, 196 murrah buffalo cows were genotyped by pcr. the association of the marker with total milk, ... | 2015 | 25726116 |
| generation of bovine (bos indicus) and buffalo (bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality. | adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. in this study, we report on the isolation and characterization of mesenchymal stem cells (msc) derived from bovine and buffalo adipose tissue. cells isolated using enzymatic digestion of bovine and buffalo adipose-tissue biopsy samples were grown in vitro for at least 15 passages, verifyi ... | 2015 | 25729935 |
| characterisation of a babesia orientalis apical membrane antigen, and comparison of its orthologues among selected apicomplexans. | in the present study, we identified and characterised the complete coding sequence of babesia orientalis apical membrane antigen 1 (designated bo-ama1); it is 1803bp in length and encodes a polypeptide of 601 amino acids (aa). the bo-ama-1 gene product (bo-ama1) is predicted to be 67kda in size and contains a signal peptide. mature bo-ama1 is predicted to have one transmembrane region and a short cytoplasmic tail (c-terminal domain). the extracellular part of bo-ama1 has three functional domains ... | 2015 | 25732411 |
| investigation of anti-toxoplasma gondii antibodies in water buffaloes (bubalus bubalis) in samsun and afyon provinces. | the present study was conducted to investigate the seroprevalence of toxoplasma gondii infection in water buffaloes. | 2014 | 25732878 |
| g-protein-coupled chemokine receptor gene in lumpy skin disease virus isolates from cattle and water buffalo (bubalus bubalis) in egypt. | lumpy skin disease virus (lsdv), sheep poxvirus (spv) and goat poxvirus (gpv) are the most serious poxviruses of ruminants. in this study, we analysed the g-protein-coupled chemokine receptor (gpcr) genes of lsdv isolates from cattle and water buffalo (bubalus bubalis) in egypt during the summer of 2011. multiple alignments of the nucleotide sequences revealed that the water buffalo lsdv isolate differed from the cattle isolate at four nucleotide positions, and both isolates had nine nucleotide ... | 2016 | 25754131 |
| transcriptional dynamics of homeobox c11 gene in water buffalo bubalus bubalis. | the hox complex contains 39 genes clustered into four groups involved in cell differentiation and development. we cloned full-length sequence of hoxc11 gene from water buffalo bubalus bubalis, assessed its copy number, localized the same onto the chromosome 5, and studied its evolutionary conservation across the species. northern hybridization of hoxc11 showed a 2.2 kb band in the tissues analyzed. real-time pcr showed highest expression of hoxc11 gene in lung followed by spleen, spermatozoa, an ... | 2015 | 25760398 |
| dietary inorganic chromium in summer-exposed buffalo calves (bubalus bubalis): effects on biomarkers of heat stress, immune status, and endocrine variables. | the aim of this study was to evaluate the effect of different levels of inorganic chromium (cr) on heat stress, immune response, and hormonal variation in murrah buffalo calves during the summer season. twenty-four growing murrah buffalo calves were randomly allocated into four treatments for a period of 120 days. feeding regimen was same in all the groups, except the buffalo calves in treatment groups were additionally supplemented with 0.5, 1.0, and 1.5 mg of inorganic cr/kg dry matter. buffal ... | 2015 | 25762098 |
| seasonal variations of the ovarian activity and pregnancy rate in the egyptian buffalo cows (bubalus bubalis). | the objective of this study was to investigate the effect of season on the follicular and luteal dynamics and pregnancy rate in egyptian buffaloes. a total of 327 genital tracts and 596 animals were used. the genital tracts were examined in winter (n = 58), spring (n = 179), summer (n = 49), and autumn (n = 41) for follicular population, incidence of presence of developed or mature corpus luteum (cl), and diameters of the preovulatory follicle (of) and the mature cl. buffaloes were mated in wint ... | 2015 | 25778728 |
| genetic analysis of river, swamp and hybrid buffaloes of north-east india throw new light on phylogeography of water buffalo (bubalus bubalis). | this study analysed buffaloes from north-east india and compared their nuclear and mitochondrial dna variations with buffaloes of mainland india, china, mediterranean and south-east asia. microsatellite genotypes of 338 buffaloes including 210 from six north-east indian buffalo populations and three mainland indian breeds were analysed to evaluate their genetic structure and evolutionary relationships. phylogenetic analysis and multidimensional scaling plot of pairwise fst revealed the clusterin ... | 2015 | 25780854 |
| comparative genomic analysis of buffalo (bubalus bubalis) nod1 and nod2 receptors and their functional role in in-vitro cellular immune response. | nucleotide binding and oligomerization domain (nod)-like receptors (nlrs) are innate immune receptors that recognize bacterial cell wall components and initiate host immune response. structure and function of nlrs have been well studied in human and mice, but little information exists on genetic composition and role of these receptors in innate immune system of water buffalo--a species known for its exceptional disease resistance. here, a comparative study on the functional domains of nod1 and n ... | 2015 | 25786158 |
| effect of donor cell type on developmental competence, quality, gene expression, and epigenetic status of interspecies cloned embryos produced using cells from wild buffalo and oocytes from domestic buffalo. | this study compared the cloning efficiency of donor cells of fibroblast and epithelial origin isolated from ear skin of a wild buffalo (bubalus arnee) and used with cytoplasts from domestic buffalo (bubalus bubalis) in interspecies scnt by hand-made cloning. the cleavage (93.0 ± 2.8% vs. 85.6 ± 2.4%) and blastocyst rates (50.6 ± 4.0% vs. 20.5 ± 2.6%) were higher (p < 0.05) for fibroblasts than those for epithelial cells, whereas the total cell number (490 ± 42 and 492 ± 95, respectively) and apo ... | 2015 | 25799471 |
| evaluation of thermal comfort, physiological, hematological, and seminal features of buffalo bulls in an artificial insemination station in a tropical environment. | this study aimed to assess the variation over time in thermal comfort indices and the behavior of physiological parameters related to thermolysis, blood parameters, and semen in natura of buffalo bulls reared in tropical climate. the study was carried out in an artificial insemination station under a humid tropical climate (afi according to köppen). ten water buffalo bulls (bubalus bubalis) were used during the 5 months (april to august) of study. the environmental temperature humidity index (th ... | 2015 | 25801015 |
| sustained delivery of exogenous melatonin influences biomarkers of oxidative stress and total antioxidant capacity in summer-stressed anestrous water buffalo (bubalus bubalis). | high ambient temperature during summer in tropical and subtropical countries predisposes water buffaloes (bubalus bubalis) to develop oxidative stress having antigonadotropic and antisteroidogenic actions. melatonin is a regulator of seasonal reproduction in photoperiodic species and highly effective antioxidant and free radical scavenger. therefore, a study was designed to evaluate the effect of sustained-release melatonin on biomarkers of oxidative stress i.e., the serum malondialdehyde (mda) ... | 2015 | 25805692 |
| downregulation of dna methyltransferase 1 in zona-free cloned buffalo (bubalus bubalis) embryos by small interefering rna improves in vitro development but does not alter dna methylation level. | aberrant epigenetic reprogramming, especially genomic hypermethylation, is implicated as the primary reason behind the failure of the cloning process during somatic cell nuclear transfer (scnt). we transfected one-cell-stage zona-free buffalo embryos produced by handmade cloning with 50 nm dnmt1 small interfering rna (sirna), using lipofectamine, to knockdown the dna methyltransferase 1 (dnmt1) gene. sirna treatment decreased (p<0.001) the expression level of dnmt1 mrna and dnmt1 protein in the ... | 2015 | 25826721 |
| molecular detection of coxiella burnetii using an alternative loop-mediated isothermal amplification assay (lamp). | q fever, caused by coxiella burnetii, is a worldwide zoonosis with important consequences for human and animal health. in livestock, the diagnosis, using direct and indirect techniques, is challenging even if to tackle coxiellosis in domesticated animals a rapid diagnosis is crucial. in the recent years, new molecular methods have been developed to overcome these issues. several polymerase chain reaction (pcr) assays have been studied, but loop mediated isothermal amplification (lamp) has not be ... | 2015 | 25842216 |
| prevalence study and genetic typing of bovine viral diarrhea virus (bvdv) in four bovine species in china. | to determine the nationwide status of persistent bvdv infection in different bovine species in china and compare different test methods, a total of 1379 serum samples from clinical healthy dairy cattle, beef cattle, yaks (bos grunniens), and water buffalo (bubalus bubalis) were collected in eight provinces of china from 2010 to 2013. the samples were analyzed using commercial antibody (ab) and antigen (ag) detection kits, and rt-pcr based on the 5'-utr and npro gene sequencing. results showed th ... | 2015 | 25849315 |
| efficiency of antifreeze glycoproteins for cryopreservation of nili-ravi (bubalus bubalis) buffalo bull sperm. | experiments were conducted to evaluate the effect of antarctic fish antifreeze glycoproteins, (afgp) size 1-5 (34-10.5 kda) and 7-8 (3.2 and 2.4 kda) in extender on buffalo bull sperm at cooling (4 °c) and at post thawing. semen was collected from three nili-ravi buffalo bulls with artificial vagina for 3 weeks. qualifying ejaculates from each buffalo bull were diluted (at 37 °c having 50×10(6) sperm/ml) in tris-citric acid extender containing afgp at 0 (control), 0.1, 1 and 10 μg/ml. an aliquot ... | 2015 | 25863987 |
| identification, molecular characterization, and tissue expression of parathyroid hormone-related protein gene (pthrp) from water buffalo (bubalus bubalis). | parathyroid hormone-related protein (pthrp) is involved in the deposition of milk calcium in mammal lactation, but its role in buffalo is unclear. in this study, the full-length coding sequence of the water buffalo pthrp gene was first isolated using reverse transcription-polymerase chain reaction. the protein was then subjected to molecular characterization using bioinformatic methods, and the tissue expression pattern was further assayed by semi-quantitative reverse-transcription polymerase ch ... | 2015 | 25867375 |
| cholesterol and fatty acid composition of longissimus thoracis from water buffalo (bubalus bubalis) and brahman-influenced cattle raised under savannah conditions. | male (n=66) water buffalo (buffalo) and brahman-influenced cattle (brahman) were born, raised, weaned, fattened on grazing savannah and harvested at two different ages (19 and 24months) to compare lipid composition of the longissimus thoracis muscle. half of the animals were castrated at seven months of age (moa) to examine the castration effects. at 24 moa brahman steers showed the highest content of total lipids (p<0.05). no significant variation was detected in cholesterol content for either ... | 2015 | 25879797 |
| mitochondrial dna variability of domestic river buffalo (bubalus bubalis) populations: genetic evidence for domestication of river buffalo in indian subcontinent. | river buffalo, bubalus bubalis is a large bovine species frequently used livestock in southern asia. it is believed that the river buffalo was domesticated from bubalus arnee, the wild buffalo of mainland asia, a few thousand years ago, probably during the period of indus valley civilization. however, the domestication history of the river buffalo has been the subject of debate for many decades mainly due to the lack of clear archeological evidence and the divisive conclusions of the genetic stu ... | 2015 | 25900921 |
| development of an indirect elisa using different fragments of recombinant ncgra7 for detection of neospora caninum infection in cattle and water buffalo. | dense granules are immunodominant proteins for the standardization of immunodiagnostic procedures to detect neosporosis. in the presented study different fragment of a dense-granule protein was evaluated for serodiagnosis of neospora caninum in cattle and water buffalo. | 2017 | 25904948 |
| biocomputational characterization and evolutionary analysis of bubaline dicer1 enzyme. | dicer, an ribonuclease type iii type endonuclease, is the key enzyme involved in biogenesis of micrornas (mirnas) and small interfering rnas (sirnas), and thus plays a critical role in rna interference through post transcriptional regulation of gene expression. this enzyme has not been well studied in the indian water buffalo, an important species known for disease resistance and high milk production. in this study, the primary coding sequence (5,778 bp) of bubaline dicer (genbank: ab969677.1) w ... | 0 | 25925065 |
| molecular phylogenetic analysis of fasciola flukes from eastern india. | fasciola flukes from eastern india were characterized on the basis of spermatogenesis status and nuclear its1. both fasciola gigantica and aspermic fasciola flukes were detected in imphal, kohima, and gantoku districts. the sequences of mitochondrial nad1 were analyzed to infer their phylogenetical relationship with neighboring countries. the haplotypes of aspermic fasciola flukes were identical or showed a single nucleotide substitution compared to those from populations in the neighboring coun ... | 2015 | 25936970 |
| identification of appropriate reference genes for qrt-pcr analysis of heat-stressed mammary epithelial cells in riverine buffaloes (bubalus bubalis). | gene expression studies require appropriate normalization methods for proper evaluation of reference genes. to date, not many studies have been reported on the identification of suitable reference genes in buffaloes. the present study was undertaken to determine the panel of suitable reference genes in heat-stressed buffalo mammary epithelial cells (mecs). briefly, mec culture from buffalo mammary gland was exposed to 42 °c for one hour and subsequently allowed to recover at 37 °c for different ... | 2013 | 25937980 |
| relationship between seminal plasma tuberoinfundibular peptide of 39 residues and sperm functional attributes in buffalo (bubalus bubalis). | the buffalo seminal plasma protein profile and its relationship with sperm quality have not been studied in detail. thus, the aim of the present study was to profile buffalo seminal plasma proteins and to assess the relationship between differentially expressed proteins and sperm characteristics. semen samples (n = 44) were collected from 11 murrah buffalo bulls (four ejaculates from each animal) and seminal plasma protein profiling was performed by sodium dodecyl sulfate-polyacrylamide gel elec ... | 2015 | 25940576 |
| localization of igf proteins in various stages of ovarian follicular development and modulatory role of igf-i on granulosa cell steroid production in water buffalo (bubalus bubalis). | the present study aimed to determine the expression of insulin like growth factor (igf) genes in the bubaline ovarian follicles and modulatory role of igf-i on progesterone production from granulosa cells (gc) of pre-ovulatory follicle in vitro. according to size, follicles were classified into four groups: gi (small), gii (medium), giii (large) and giv (preovulatory). all igf genes were expressed in both gc and theca interna (ti) cells. the relative expression of igf-i and igf receptor i (igfr- ... | 2015 | 25951771 |
| comparison of the tuberculin test, histopathological examination, and bacterial culture for the diagnosis of tuberculosis (mycobacterium bovis) in buffaloes (bubalus bubalis) in brazil. | tuberculosis is a disease with a great zoonotic potential. it is considered a major obstacle to cattle production and is responsible for severe losses in several production systems. a comparative cervical test (cct) was performed in 1140 buffaloes from different mesoregions of the state of pará, brazil, with the aim of comparing the sensitivity and specificity of cct with histopathological examination and bacterial culture. of the animals tested using cct, 4.65% (53/1140) were positive, 2.98% (3 ... | 2015 | 25958174 |
| molecular analyses detect natural coinfection of water buffaloes (bubalus bubalis) with bovine viral diarrhea viruses (bvdv) in serologically negative animals. | infection of water buffaloes (bubalus bubalis) with bovine viral diarrhea viruses (bvdv) has been confirmed in several studies by serological and molecular techniques. in order to determine the presence of persistently infected animals and circulating species and subtypes of bvdv we conducted this study on a buffalo herd, whose habitat was shared with bovine cattle (bossp.). our serological results showed a high level of positivity for bvdv-1 and bvdv-2 within the buffalo herd. the molecular ana ... | 2015 | 25962538 |
| expression profiling of major heat shock protein genes during different seasons in cattle (bos indicus) and buffalo (bubalus bubalis) under tropical climatic condition. | heat shock proteins consist of highly conserved stress proteins, expressed in response to stress and play crucial roles in environmental stress tolerance and adaptation. the present study was conducted to identify major types of genes under the hsp70 family and other hsps and to evaluate their expression pattern in sahiwal and tharparkar breeds of zebu cattle (bos indicus) and murrah buffalo (bubalus bubalis) with respect to different seasons. quantitative real time polymerase chain reaction was ... | 2015 | 25965018 |
| molecular characterization of a new microvariant of the g3 genotype for echinococcus granulosus in water buffalo in iran. | in this study, molecular characterization of echinococcus granulosus sample obtained from water buffalo originating from southwest of iran was performed using comparative sequence analysis of cox1 mitochondrial gene. dna was extracted from protoscoleces removed from hydatid cyst from the liver of a 2-year-old male buffalo slaughtered in khuzestan province. molecular and phylogenetic analyses were conducted based on cox1 mitochondrial gene. we found the presence of a new microvariant of g3 genoty ... | 2015 | 25992257 |
| prevalence and distribution of neospora caninum in water buffalo (bubalus bubalis) and cattle in the northern territory of australia. | the seroprevalence of neospora caninum infection in water buffalo (bubalus bubalis) and domestic cattle in the northern territory (nt) of australia has never been determined. a total of 480 serum samples from water buffalo and 192 serum samples from cattle, collected by the nt government from 1993 through to 2001, at 18 different survey sites throughout the northern territory were tested by commercial elisa for anti-n. caninum antibodies. the water buffalo samples demonstrated a seroprevalence o ... | 2015 | 25992666 |
| somatic antigens of tropical liver flukes ameliorate collagen-induced arthritis in wistar rats. | parasitic helminths polarize immune response of their vertebrate hosts towards anti-inflammatory th2 type and therefore it is hypothesized that they may suppress the inflammatory conditions in autoimmune disorders. the present study was undertaken to investigate in vivo immunomodulatory and therapeutic potential of somatic antigens (ag) of liver infecting digenetic trematodes [fasciola gigantica (fg) and gigantocotyle explanatum (ge)] in collagen-induced arthritic (cia) wistar rats. the cia rats ... | 2015 | 25992888 |
| isolation, characterization and functional analysis of full length p53 cdna from bubalus bubalis. | p53 plays a pivotal role in maintaining the genomic integrity of the cell and has an important role in cellular transformation. we isolated and cloned a full length p53 cdna (bp53) from water buffalo in expression vectors designed to generate tagged proteins with flag or gfp. bp53 was found to be 1161 nucleotide long and codes for 386 amino acid residues with 79% homology with human p53 containing 393 amino acids. although bp53 has some inherent differences in amino acid composition in different ... | 2015 | 26003295 |
| chromium supplements in the feed for lactating murrah buffaloes (bubalus bubalis): influence on nutrient utilization, lactation performance, and metabolic responses. | this study was conducted to evaluate the effect of different levels of inorganic chromium (cr) on nutrient intake, nutrient utilization, milk mineral contents, and blood biochemical in lactating murrah buffaloes. twenty-four multifarious murrah buffaloes were blocked into four groups having six animals in each group and fed for 150 days post-partum. feeding regimen was same in all the groups except that treatment groups were supplemented with 0.0, 0.5, 1.0, and 1.5 mg inorganic cr/kg dry matter ... | 2015 | 26013395 |
| buffalo (bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro. | recent studies suggested that placentae amniotic membrane is a valuable source of stem cells in human as well as in livestock species. advantages of amnion over other sources of stem cells included abundant availability, ethically non-objectionable and non-invasive source. the aim of the present study was the isolation, culture and characterization of amniotic-membrane-derived mesenchymal stem cells from term placentae collected postpartum in buffalo. we have observed that both presumptive epith ... | 2015 | 26019121 |
| effect of pour-on alphacypermethrin on feed intake, body condition score, milk yield, pregnancy rates, and calving-to-conception interval in buffaloes. | the aims of this study were to assess the efficacy of alphacypermethrin (acyp) on pediculosis due to haematopinus tuberculatus and to evaluate the influence of the treatment on productive and reproductive performance in buffaloes (bubalus bubalis) reared in an intensive system. the trial was performed on 56 pluriparous buffaloes at 86.8 ± 8.1 d in milk. the animals underwent individual louse count and were divided into 2 homogenous groups according to louse count, age, number of lactations, days ... | 2015 | 26020206 |
| molecular characterisation of three regions of the nuclear ribosomal dna unit and the mitochondrial cox1 gene of sarcocystis fusiformis from water buffaloes (bubalus bubalis) in egypt. | a total of 33 macroscopically visible (3-11 × 1-5 mm) sarcocysts of sarcocystis fusiformis were excised from the oesophagus of 12 freshly slaughtered water buffalos in giza, egypt. genomic dna was extracted from the sarcocysts, and all isolates were characterised at the mitochondrial cytochrome c oxidase subunit i (cox1) gene through pcr amplification and direct sequencing, whereas a few selected isolates were characterised at the 18s and 28s ribosomal (r) rna genes and the internal transcribed ... | 2015 | 26051128 |
| production of a cloned buffalo (bubalus bubalis) calf from somatic cells isolated from urine. | this study was aimed at isolation of cells from urine and skin on the ventral part of the tails of healthy adult female buffaloes (bubalus bubalis), an area rarely exposed to solar radiation, establishment of the cells in culture, and their use as donor cells for production of buffalo embryos by handmade cloning (hmc). the blastocyst rate and total cell number of urine- and tail skin-derived embryos were similar to those of control embryos derived from ear skin cells; however, their apoptotic in ... | 2015 | 26053516 |
| establishment of trophectoderm cell lines from buffalo (bubalus bubalis) embryos of different sources and examination of in vitro developmental competence, quality, epigenetic status and gene expression in cloned embryos derived from them. | despite being successfully used to produce live offspring in many species, somatic cell nuclear transfer (nt) has had a limited applicability due to very low (>1%) live birth rate because of a high incidence of pregnancy failure, which is mainly due to placental dysfunction. since this may be due to abnormalities in the trophectoderm (te) cell lineage, te cells can be a model to understand the placental growth disorders seen after nt. we isolated and characterized buffalo te cells from blastocys ... | 2015 | 26053554 |
| liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (bubalus bubalis) semen. | demand for alternative of egg yolk in freezing extenders have increased in recent years due to variability in egg yolk composition, risk of microbial contamination and presence of steroid hormones. the alternative to egg yolk-based extender (ey) can be soya lecithin-based extender (sl) and liposome-based extender (lp). however, the efficacy of sl is still a matter of debate. few studies have been performed on the effect of lp but to date evaluation of buffalo semen cryopreserved in lp has not be ... | 2015 | 26065809 |
| cross talk between kgf and kitlg proteins implicated with ovarian folliculogenesis in buffalo bubalus bubalis. | molecular interactions between mesenchymal-derived keratinocyte growth factor (kgf) and kit ligand (kitlg) are essential for follicular development. these factors are expressed by theca and granulosa cells. we determined full length coding sequence of buffalo kgf and kitlg proteins having 194 and 274 amino acids, respectively. the recombinant kgf and kitlg proteins were solubilized in 10 mm tris, ph 7.5 and 50 mm tris, ph 7.4 and purified using ni-nta column and gst affinity chromatography, resp ... | 2015 | 26083339 |
| maldi-tof-ms platform for integrated proteomic and peptidomic profiling of milk samples allows rapid detection of food adulterations. | adulteration of ovine, caprine, and buffalo milks with more common bovine material occurs for economic reasons and seasonal availability. frauds are also associated with the use of powdered milk instead of declared, fresh material. in this context, various analytical methods have been adapted to dairy science applications with the aim to evaluate adulteration of milk samples, although time-consuming, suitable only for speciation or thermal treatment analysis, or useful for a specific fraud type. ... | 2015 | 26098723 |
| sarcocystis rommeli, n. sp. (apicomplexa: sarcocystidae) from cattle (bos taurus) and its differentiation from sarcocystis hominis. | cattle (bos taurus) are intermediate hosts for three named species of sarcocystis, s. cruzi, s. hirsuta, and s. hominis. recently, a fourth species was identified and named s. sinensis. however, s. sinensis originally named a species of sarcocystis in water buffalo (bubalus bubalis) in china. based on unverifiable evidence, it was suggested that the same parasite infects cattle. in addition, s. sinensis was recently declared as nomen nudum because its naming violated the rules of international c ... | 2016 | 26111603 |
| molecular identification and seasonal infections of species of fasciola in ruminants from two provinces in china. | we determined the prevalence and seasonality of infections by fasciola of goats and bovine species (cattle and water buffalo) in hubei and anhui provinces of china. faecal samples were collected at 2- to 3-month intervals from 200 goats in hubei province and from 152 bovine species in anhui province. all faecal samples were examined for the presence of parasites. we determined the nucleotide sequences of the first and second internal transcribed spacers (its-1 and its-2) of the nuclear ribosomal ... | 2016 | 26123576 |
| the differential expression of immune genes between water buffalo and yellow cattle determines species-specific susceptibility to schistosoma japonicum infection. | water buffalo are less susceptible to schistosoma japonicum infection than yellow cattle. the factors that affect such differences in susceptibility remain unknown. a bos taurus genome-wide gene chip was used to analyze gene expression profiles in the peripheral blood of water buffalo and yellow cattle pre- and post-infection with s. japonicum. this study showed that most of the identified differentially expressed genes (degs) between water buffalo and yellow cattle pre- and post-infection were ... | 2015 | 26125181 |
| molecular characterization and expression of buffalo (bubalus bubalis) dead-box family vasa gene and mrna transcript variants isolated from testis tissue. | vasa is a member of the dead-box protein family that plays an indispensable role in mammalian spermatogenesis, particularly during meiosis. in the present study, we isolated, sequenced, and characterized vasa gene in buffalo testis. here, we demonstrated that vasa mrna is expressed as multiple isoforms and uses four alternative transcriptional start sites (tsss) and four different polyadenylation sites. the tsss identified by 5'-rna ligase-mediated rapid amplification of cdna ends (rlm-5'-race) ... | 2015 | 26127001 |
| a diagnostic protocol to identify water buffaloes (bubalus bubalis) vaccinated with brucella abortus strain rb51 vaccine. | the use of live vaccine strain rb51 for vaccination of domestic water buffaloes (bubalus bubalis) at risk of infection with brucella abortus is permitted notwithstanding the plans for the eradication and only under strict veterinary control. the antibodies induced by rb51 vaccination are not detectable using conventional diagnostic techniques; therefore, it is necessary to have a specific diagnostic tool able to discriminate vaccinated from unvaccinated animals. the combination of a complement f ... | 2015 | 26129660 |
| ocular fluid as a replacement for serum in cell cryopreservation media. | cryostorage is of immense interest in biomedical research, especially for stem cell-based therapies and fertility preservation. several protocols have been developed for efficient cryopreservation of cells and tissues, and a combination of dimethyl sulfoxide (dmso) and fetal bovine serum (fbs) is commonly used. however, there is a need for an alternative to fbs because of ethical reasons, high cost, and risk of contamination with blood-borne diseases. the objective of the present study was to ex ... | 2015 | 26135924 |
| development, characterization, and pluripotency analysis of buffalo (bubalus bubalis) embryonic stem cell lines derived from in vitro-fertilized, hand-guided cloned, and parthenogenetic embryos. | we present the derivation, characterization, and pluripotency analysis of three buffalo embryonic stem cell (buesc) lines, from in vitro-fertilized, somatic cell nuclear-transferred, and parthenogenetic blastocysts. these cell lines were developed for later differentiation into germ lineage cells and elucidation of the signaling pathways involved. the cell lines were established from inner cell masses (icms) that were isolated manually from the in vitro-produced blastocysts. most of the icms (45 ... | 2015 | 26168169 |
| identification and characterization of a novel 34 kda merozoite protein in babesia orientalis. | a novel babesia orientalis 34 kda protein (designated bop34) was obtained by immunoscreening of a cdna expression library using b. orientalis infected water buffalo serum. the complete nucleotide sequence of the bop34 was 1088 bp, which contained one open reading frame (orf), two untranslated regions (utrs) and a poly (a) tail. the length of orf was 933 bp, encoding a polypeptide of 310 aa with a predicted size of 34 kda. blast analysis showed that the nucleotide sequence of bop34 had 71% simila ... | 2015 | 26169218 |
| water buffalo as sentinel animals for schistosomiasis surveillance. | 2015 | 26170512 | |
| molecular detection of bovine immunodeficiency virus in water buffaloes (bubalus bubalis) from the amazon region, brazil. | bovine immunodeficiency is a chronic progressive disease caused by a lentivirus that affects cattle and buffaloes. although the infection has been described in cattle in some countries, including in brazil, there are only two reports of infection in buffaloes: one in pakistan and one in cambodia. the aim of the present study was to survey the occurrence of bovine immunodeficiency virus (biv) in water buffaloes from the amazon region, pará state, brazil. biv proviral dna was surveyed in 607 whole ... | 2015 | 26174574 |
| anthelmintic effect of biocompatible zinc oxide nanoparticles (zno nps) on gigantocotyle explanatum, a neglected parasite of indian water buffalo. | helminth parasites of veterinary importance cause huge revenue losses to agrarian economy worldwide. with the emergence of drug resistance against the current formulations, there is a need to focus on the alternative approaches in order to control this menace. in the present study, biocompatible zinc oxide nanoparticles (zno nps) were used to see their in vitro effect on the biliary amphistomes, gigantocotyle explanatum, infecting bubalus bubalis because these nanoparticles are involved in gener ... | 2015 | 26177503 |
| characterization of arcobacter suis isolated from water buffalo (bubalus bubalis) milk. | during a survey in a dairy plant in italy, the second strain (strain fg 206) of arcobacter suis described in the literature was isolated from raw water buffalo milk. the objective of this study was to confirm the species identification, better define the species by comparing its characteristics with those of the reference strain (f41(t) = cect 7833(t) = lmg 26152(t)) and to investigate its potential clinical relevance by detecting the virulence gene pattern of the new strain. phenotypical charac ... | 2015 | 26187844 |
| genome-wide survey and analysis of microsatellite sequences in bovid species. | microsatellites or simple sequence repeats (ssrs) have become the most popular source of genetic markers, which are ubiquitously distributed in many eukaryotic and prokaryotic genomes. this is the first study examining and comparing ssrs in completely sequenced genomes of the bovidae. we analyzed and compared the number of ssrs, relative abundance, relative density, guanine-cytosine (gc) content and proportion of ssrs in six taxonomically different bovid species: bos taurus, bubalus bubalis, bos ... | 2015 | 26196922 |
| first report of natural bohv-1 infection in water buffalo. | 2015 | 26198212 | |
| potential risk factors associated with ill-thrift in buffalo calves (bubalus bubalis) raised at smallholder farms in egypt. | failure to grow (ill-thrift) in calves has a negative effect on animal production and health. the present study was carried out from november, 2009 to may, 2013 to investigate the risk factors of ill-thrift in buffalo calves. a total of 344 calves at 78 smallholder farms were selected randomly. a questionnaire was designed to include managemental, nutritional and disease risk factors. serum selenium, copper, zinc, iron, calcium, phosphorus and magnesium were measured. data were subjected to logi ... | 2015 | 26199751 |
| optimization of buffalo (bubalus bubalis) embryonic stem cell culture system. | in order to retain an undifferentiated pluripotent state, embryonic stem (es) cells have to be cultured on feeder cell layers. however, use of feeder layers limits stem cell research, since experimental data may result from a combined es cell and feeder cell response to various stimuli. | 2015 | 26199905 |
| metaphylactic treatment strategies with toltrazuril and diclazuril and growth performance of buffalo calves exposed to a natural eimeria infection. | five controlled field trials were conducted in southern italy to evaluate the effect of metaphylactic treatment strategies of toltrazuril and diclazuril for the control of coccidiosis in water buffaloes naturally infected by eimeria spp. the 5 farms were divided into two types (a and b) according to their management system (individual or collective breeding of buffalo calves). in the farms of type a (no. 3), the buffalo calves were bred in individual boxes from the birth to the 7th/8th week of a ... | 2015 | 26215929 |
| genetic evaluation of dual-purpose buffaloes (bubalus bubalis) in colombia using principal component analysis. | genealogy and productive information of 48621 dual-purpose buffaloes born in colombia between years 1996 and 2014 was used. the following traits were assessed using one-trait models: milk yield at 270 days (my270), age at first calving (afc), weaning weight (ww), and weights at the following ages: first year (w12), 18 months (w18), and 2 years (w24). direct additive genetic and residual random effects were included in all the traits. maternal permanent environmental and maternal additive genetic ... | 2015 | 26230093 |
| short communication: arcobacter butzleri and arcobacter cryaerophilus survival and growth in artisanal and industrial ricotta cheese. | ricotta cheese is a ready-to-eat product with properties (ph >6.0, aw >0.98-0.99) and moisture content (75-80%) that may pose a risk to public health due to postprocess contamination by several bacterial pathogens, including arcobacters. the objective of the study was to evaluate the behavior of arcobacter butzleri and arcobacter cryaerophilus in ricotta cheese during its shelf life assuming postprocessing contamination. two types of ricotta cheese, artisanal water buffalo (wb) and industrial co ... | 2015 | 26233450 |
| [analysis and identification of water soluble components of water buffalo horn]. | this study is to analyze and identify the water soluble components of water buffalo horn (bubali cornu, wbh), and also establish a method for investigating these components. shotgun proteomic analysis identified proteins in wbh aqueous extraction: keratin, collagen, desmoglein, etc. ultrafiltration and lc-ms/ms were used to separate and identify the peptides in wbh aqueous extract, as a result, identified peptides were mainly derived from nonspecific degradation products of keratin and collagen, ... | 2015 | 26234143 |
| glycome characterization of immunoglobulin g from buffalo (bubalus bubalis) colostrum. | immunoglobulin g (igg) is a major glycoprotein in ruminant colostrum. first day buffalo colostrum protein was purified on sephadex g-100 and its mass was determined by maldi-tof as 147.848 kda. the pmf data of protein subunits revealed its homology to igg, which was supported by the identification of peptide sequences lliygatsr and vyneylpapivr corresponding to light and heavy chains of igg by cid ms/ms analysis. the n-glycan microheterogeneity was established based on chemoselective glycoblotti ... | 2015 | 26239923 |
| controlled breeding and reproductive management in water buffaloes (bubalus bubalis) using eazi breed controlled internal drug release. | buffalo reproduction is considerably affected by late maturity, poor oestrus symptoms and long postpartum periods. this study was undertaken to evaluate the efficiency of eazi breed controlled internal drug release (cidr), an intravaginal progesterone-releasing device, in relation to oestrus and fertility. five hundred true anoestrus buffalo cows, in the age group 4-6 years in 10 villages of dharwad district in karnataka state in india, were randomly selected and treated with cidr for 9 days. tw ... | 2015 | 26244580 |
| evaluation of lipid peroxidation and antioxidant status on fenvalerate, nitrate and their co-exposure in bubalus bubalis. | the toxic effects of pesticides and minerals have been explored in different species, but still there is paucity of information regarding their combined toxicological effects. the present investigation reports oxidative stress induced by oral subacute exposure to fenvalerate (1 mg/kg) and sodium nitrate (20 mg/kg) alone, as well as in combination daily for 21 days in buffalo calves. fenvalerate exposure produced significant elevation in lipid peroxidation (lpo), glutathione peroxidase (gpx), whi ... | 2015 | 26267048 |
| association analysis of polymorphism in thyroglobulin gene promoter with milk production traits in riverine buffalo (bubalus bubalis). | polymorphism within the promoter region of bovine thyroglobulin has been reported to be associated with milk and meat quality. in this study, we investigated the genetic variation within thyroglobulin promoter region of swamp and riverine buffaloes using pcr-sscp technique and sequencing, and also analyzing association of polymorphism with the milk production traits. the study revealed four conformational patterns, a, b, c, and d among 323 buffaloes of two riverine breeds and different swamp pop ... | 2015 | 26273563 |
| comparative analysis of transcriptional profiles of adult schistosoma japonicum from different laboratory animals and the natural host, water buffalo. | schistosomiasis is one of the most widely distributed parasitic diseases in the world. schistosoma japonicum, a zoonotic parasite with a wide range of mammalian hosts, is one of the major pathogens of this disease. although numerous studies on schistosomiasis japonica have been performed using laboratory animal models, systematic comparative analysis of whole-genome expression profiles in parasites from different laboratory animals and nature mammalian hosts is lacking to date. | 2015 | 26285138 |
| a review of neospora caninum in water buffalo (bubalus bubalis). | a number of countries in the world have reported infections with neospora caninum in water buffalo (bubalus bubalis), from africa to asia, europe and south america and recently australia. in general, clinical manifestations (such as abortion) seem rare, which has raised the prospect that buffalo may be inherently resistant to clinical effects of n. caninum infection. worldwide, the seroprevalence of n. caninum infection (as a measure of exposure determined by the detection of antibody) in buffal ... | 2015 | 26298507 |
| modification of the kjeldahl noncasein nitrogen method to include bovine milk concentrates and milks from other species. | the objective of our research was to modify the current indirect casein method for bovine milk to enable it to be applied to bovine milk, bovine milk concentrates, and milks of other species that contain a protein concentration up to 9% (wt/wt). our work used a series of bovine milk concentrates from about 3 to 9% protein with the same casein as a percentage of true protein to determine the amount of buffer required and ph of the noncasein nitrogen (ncn) filtrate to achieve consistent estimates ... | 2015 | 26298747 |
| correction: genetic evaluation of dual-purpose buffaloes (bubalus bubalis) in colombia using principal component analysis. | 2015 | 26312483 | |
| the prion protein gene polymorphisms associated with bovine spongiform encephalopathy susceptibility differ significantly between cattle and buffalo. | prion protein, encoded by the prion protein gene (prnp), plays a crucial role in the pathogenesis of transmissible spongiform encephalopathies (tses). several polymorphisms within the prnp are known to be associated with influencing bovine spongiform encephalopathy (bse) susceptibility in cattle, namely two insertion/deletion (indel) polymorphisms (a 23-bp indel in the putative promoter and a 12-bp indel in intron 1), the number of octapeptide repeats (octarepeats) present in coding sequence (cd ... | 2015 | 26319996 |
| handmade cloned buffalo (bubalus bubalis) embryos produced from somatic cells isolated from milk and ear skin differ in their developmental competence, epigenetic status, and gene expression. | we compared the cloning efficiency of buffalo embryos produced by handmade cloning (hmc) using ear skin- and milk-derived donor cells. the blastocyst rate was lower (p < 0.05) for milk-derived than that for skin-derived embryos, whereas the total cell number and apoptotic index were similar. the global level of h3k9ac was higher (p < 0.05) in skin- than in milk-derived cells, whereas the level of h3k27me3 was similar in the two groups. the global level of h3k9ac was similar between milk-derived ... | 2015 | 26332294 |
| volume calculation of the cattle (bos taurus l.) and the water buffalo (bos bubalis l.) metapodia with stereologic method. | in this study, stereological volume estimations using 26 cattle metapodia (26 metacarpal and 26 metatarsal bones) and 8 water buffalo metapodia (8 metacarpal and 8 metatarsal bones) were made. for this purpose metapodia were parallel sectioned at 1 cm intervals according to cavalieri principle. grids with 0.4 cm probe intervals were superimposed on top of these sections and the matching points were counted. all of the bone structures and medullar cavity volumes were calculated with the data obta ... | 2015 | 26339814 |
| discordance between in silico & in vitro analyses of ace inhibitory & antioxidative peptides from mixed milk tryptic whey protein hydrolysate. | ace inhibitory and antioxidative peptides identified by lcms/ms, from mixed milk (bubalus bubalis and bos taurus) tryptic whey protein hydrolysate, were compared with the in silico predictions. α la and ß lg sequences, both from bubalus bubalis and bos taurus, were used for in silico study. swiss-prot and biopep protein libraries were accessed for prediction of peptide generation. study observed gaps in the prediction versus actual results, which remain unaddressed in the literature. many peptid ... | 2014 | 26344975 |
| comparison of non-linear models to describe the lactation curves for milk yield and composition in buffaloes (bubalus bubalis). | in order to describe the lactation curves of milk yield (my) and composition in buffaloes, seven non-linear mathematical equations (wood, dhanoa, sikka, nelder, brody, dijkstra and rook) were used. data were 116,117 test-day records for my, fat (fp) and protein (pp) percentages of milk from the first three lactations of buffaloes which were collected from 893 herds in the period from 1992 to 2012 by the animal breeding center of iran. each model was fitted to monthly production records of dairy ... | 2016 | 26354679 |
| study of proteolysis in river buffalo mozzarella cheese using a proteomics approach. | the guarantee of the origin and quality of raw material is essential for the protection and valorization of campana buffalo mozzarella cheese. the risk of utilization of semifinished products and stored milk in substitution for fresh milk is increasing, due to the continuous desire to reduce production costs. a proteomics approach and electrophoresis survey of retail mozzarella cheeses indicated different rates of proteolysis in the production of dairy industries. the use of fresh milk and corre ... | 2015 | 26364106 |
| correction: correction: genetic evaluation of dual-purpose buffaloes (bubalus bubalis) in colombia using principal component analysis. | 2015 | 26367259 | |
| effect of relaxin on fertility parameters of frozen-thawed buffalo (bubalus bubalis) sperm. | the aim of this work was to evaluate the effect of relaxin on fertility parameters of buffalo frozen/thawed sperm. sperm were incubated in the absence of capacitating agents (negative control), with a known capacitating agent such as heparin (positive control) and with 50 and 100 ng/ml relaxin for 2 and 4 h. sperm viability, motility, capacitation and the effect of relaxin on the fertilizing ability after heterologous ivf were evaluated. although viability was not affected, relaxin increased (p ... | 2015 | 26372314 |
| genetic diversity analysis of buffalo fatty acid synthase (fasn) gene and its differential expression among bovines. | fatty acid synthase (fasn) gene seems to be structurally and functionally different in bovines in view of their distinctive fatty acid synthesis process. structural variation and differential expression of fasn gene is reported in buffalo (bubalus bubalis), a bovine species close to cattle, in this study. amino acid sequence and phylogenetic analysis of functionally important thioesterase (te) domain of fasn revealed its conserved nature across mammals. amino acid residues at te domain, responsi ... | 2016 | 26376068 |
| identification and genetic characterization of rabies virus from egyptian water buffaloes (bubalus bubalis) bitten by a fox. | rabies is caused by negative strand rna-virus classified in the genus lyssavirus, family rhabdoviridae of the order mononegavirales. the aim of the present study was to identify and analyze nucleotides sequence of nucleoprotein (n) gene of rabies virus (rabv) from two cases of water buffaloes (bubalus bubalis) bitten by a fox in egypt, 2013. the diseased buffaloes showed nervous manifestations with fever. specimens from brains of the buffaloes with suspected rabies were collected. rabv in collec ... | 2015 | 26396980 |