Publications

microrna screening identifies mir-134 as a regulator of poliovirus and enterovirus 71 infection.	micrornas (mirnas) regulate virus replication through multiple mechanisms. poliovirus causes a highly debilitating disease and though global efforts to eradicate polio have sharply decreased polio incidence, unfortunately three countries (afghanistan, nigeria and pakistan) remain polio-endemic. we hypothesize that understanding the host factors involved in polio replication will identify novel prophylactic and therapeutic targets against polio and related viruses. in this data set, employing gen ...	2017	28248924
a neonatal murine model of coxsackievirus a6 infection for evaluation of antiviral and vaccine efficacy.	hand, foot and mouth disease (hfmd) is a global health concern. family picornaviridae members, particularly enterovirus 71 (eva71) and coxsackievirus a16 (cva16), are the primary etiological agents of hfmd, however, a third enterovirus a species, coxsackievirusa6 (cva6), has been recently associated with epidemic outbreaks. study of the pathogenesis of cva6 infection and development of antivirals and vaccines are hindered due to the lack of appropriate animal models. we have developed and charac ...	2017	28250116
correction: enterovirus 71 protease 2apro targets mavs to inhibit anti-viral type i interferon responses.	[this corrects the article doi: 10.1371/journal.ppat.1003231.].	2017	28253362
report from the world health organization's third product development for vaccines advisory committee (pdvac) meeting, geneva, 8-10th june 2016.	the third meeting of who's product development for vaccines advisory committee (pdvac) was held in june 2016, with a remit to revisit the pathogen areas for which significant progress has occurred since recommendations from the 2015 meeting, as well as to consider new advances in the development of vaccines against other pathogens. since the previous meeting, significant progress has been made with regulatory approvals of the first malaria and dengue vaccines, and the first phase iii trials of a ...	2017	28262332
enhancing enterovirus a71 vaccine production yield by microcarrier profusion bioreactor culture.	hand, foot and mouth diseases (hfmd) are mainly caused by enterovirus a71 (ev-a71) infections. clinical trials in asia conducted with formalin-inactivated ev-a71 vaccine candidates produced from serum-free vero cell culture using either roller bottle or cell factory technology, are found to be safe and highly efficacious. to increase vaccine yields and reduce the production costs, the bioprocess improvement for ev-a71 vaccine manufacturing is currently being investigated. the parameters that cou ...	2017	28274636
the uncoupling of catalysis and translocation in the viral rna-dependent rna polymerase.	the nucleotide addition cycle of nucleic acid polymerases includes two major events: the pre-chemistry active site closure leading to the addition of one nucleotide to the product chain; the post-chemistry translocation step moving the polymerase active site one position downstream on its template. in viral rna-dependent rna polymerases (rdrps), structural and biochemical evidences suggest that these two events are not tightly coupled, unlike the situation observed in a-family polymerases such a ...	2017	28277928
paediatric active enhanced disease surveillance inaugural annual report, 2014.	the paediatric active enhanced disease surveillance (paeds) network is a hospital-based active surveillance system employing prospective case ascertainment of selected uncommon vaccine preventable diseases and potential adverse events following immunisation (aefi). paeds enhances other australian surveillance systems by providing prospective detailed clinical and laboratory data for the same child.	2016	28278415
a novel benzo-heterocyclic amine derivative n30 inhibits influenza virus replication by depression of inosine-5'-monophospate dehydrogenase activity.	influenza virus is still a huge threat to the world-wide public health. host inosine-5'- monophosphate dehydrogenase (impdh) involved in the synthesis of guanine nucleotides, is known to be a potential target to inhibit the replication of viruses. herein, we evaluated antiviral activity of a benzo-heterocyclic amine derivative n30, which was designed to inhibit impdh.	2017	28298229
best practices to prevent transmission and control outbreaks of hand, foot, and mouth disease in childcare facilities: a systematic review.	hand, foot, and mouth disease continues to cause seasonal epidemics in the asia-pacific region. since the current enterovirus 71 vaccines do not provide cross-protection for all enterovirus species that cause hand, foot, and mouth disease, there is an urgent need to identify appropriate detection tools and best practice to prevent its transmission and to effectively control its outbreaks. this systematic review aimed to identify characteristics of outbreak and assess the impact and effectiveness ...	2017	28302925
the golgi protein acbd3 facilitates enterovirus 71 replication by interacting with 3a.	enterovirus 71 (ev71) is a human pathogen that causes hand, foot, mouth disease and neurological complications. although ev71, as well as other enteroviruses, initiates a remodeling of intracellular membrane for genomic replication, the regulatory mechanism remains elusive. by screening human cdna library, we uncover that the golgi resident protein acyl-coenzyme a binding domain-containing 3 (acbd3) serves as a target of the 3a protein of ev71. this interaction occurs in cells expressing 3a or i ...	2017	28303920
squamous epitheliotropism of enterovirus a71 in human epidermis and oral mucosa.	hand-foot-and-mouth disease is a self-limiting paediatric infectious disease commonly caused by enterovirus a71 (genus: enterovirus, family: picornaviridae). typical lesions in and around the hands, feet, oral cavity and other places may rarely be complicated by acute flaccid paralysis and acute encephalomyelitis. although virus is readily cultured from skin vesicles and oral secretions, the cellular target/s of enterovirus a71 in human skin and oral mucosa are unknown. in enterovirus a71-infect ...	2017	28322333
efficacy and safety evaluation of a chlorine dioxide solution.	in this study, a chlorine dioxide solution (uc-1) composed of chlorine dioxide was produced using an electrolytic method and subsequently purified using a membrane. uc-1 was determined to contain 2000 ppm of gaseous chlorine dioxide in water. the efficacy and safety of uc-1 were evaluated. the antimicrobial activity was more than 98.2% reduction when uc-1 concentrations were 5 and 20 ppm for bacteria and fungi, respectively. the half maximal inhibitory concentrations (ic50) of h1n1, influenza vi ...	2017	28327506
enterovirus 71 neutralizing antibodies seroepidemiological research among children in guangzhou, china between 2014 and 2015: a cross-sectional study.	a hand-foot-mouth disease outbreak occurred in 2014 around guangdong. the purpose of this study was investigating the status and susceptibility of infectious neutralizing antibodies to enterovirus 71 among children so as to provide scientific evidence for the population immunity level of hand-foot-mouth disease and prepare for enterovirus 71 vaccination implementation. serum specimens were collected from children in communities from january 2014 to march 2015 in guangzhou. a total of 197 serum s ...	2017	28335535
outbreak of brainstem encephalitis associated with enterovirus-a71 in catalonia, spain (2016): a clinical observational study in a children's reference centre in catalonia.	to describe the characteristics of an outbreak of brainstem encephalitis and encephalomyelitis related to enterovirus (ev) infection in catalonia (spain), a setting where these manifestations were uncommon.	2017	28344164
regulation of enterovirus 2a protease-associated viral ires activities by the cell's erk signaling cascade: implicating erk as an efficiently antiviral target.	in a previous study the erk1/2 pathway was found to be crucially involved in positive regulation of the enterovirus a 71(ev-a71) ires (vires), thereby contributing to the efficient replication of an important human enterovirus causing death in young children (<5yrs) worldwide. this study focuses on unraveling more about the detailed mechanism of erk's involvement in this regulation of vires. through the use of sirnas and specifically pharmacological inhibitor u0126, the erk cascade was shown to ...	2017	28351508
epidemics and aetiology of hand, foot and mouth disease in xiamen, china, from 2008 to 2015.	over the past 8 years, human enteroviruses (hevs) have caused 27 227 cases of hand, foot and mouth disease (hfmd) in xiamen, including 99 severe cases and six deaths. we aimed to explore the molecular epidemiology of hfmd in xiamen to inform the development of diagnostic assays, vaccines and other interventions. from january 2009 to september 2015, 5866 samples from sentinel hospitals were tested using nested reverse transcription pcr that targeted the hev 5' untranslated region and viral protei ...	2017	28367766
impact of genetic changes, pathogenicity and antigenicity on enterovirus- a71 vaccine development.	enterovirus-a71 (ev-a71) is an etiological agent of the hand, foot and mouth disease (hfmd). ev-a71 infection produces high fever and ulcers in children. some ev-a71 strains produce severe infections leading to pulmonary edema and death. although the protective efficacy of the inactivated vaccine (iv) was ≥90% against mild hfmd, there was approximately 80% protection against severe hfmd. the monovalent ev-a71 iv elicits humoral immunity but lacks long-term immunogenicity. spontaneous mutations o ...	2017	28384566
lithium chloride inhibits early stages of foot-and-mouth disease virus (fmdv) replication in vitro.	foot-and-mouth disease virus (fmdv) causes an economically important and highly contagious disease of cloven-hoofed animals such as cattle, swine, and sheep. fmd vaccine is the traditional way to protect against the disease, which can greatly reduce its occurrence. however, the use of fmd vaccines to protect early infection is limited. therefore, the alternative strategy of applying antiviral agents is required to control the spread of fmdv in outbreak situations. as previously reported, licl ha ...	2017	28390158
a novel combined vaccine based on monochimeric vlp co-displaying multiple conserved epitopes against enterovirus 71 and varicella-zoster virus.	chicken pox and hand, foot and mouth disease (hfmd) are two major infectious diseases that mainly affect infants and children, causing significant morbidity annually. varicella-zoster virus (vzv) and enterovirus 71 (ev71), respectively, are the principal epidemic pathogens causing these two diseases. to investigate the possibility of developing a novel combined vaccine to prevent chicken pox and hfmd, we constructed three chimeric virus-like particles (vlps) (termed hbc-v/1/2, hbc-2/v/1 and hbc- ...	2017	28408118
dynamic interaction of enterovirus 71 and dendritic cells in infected neonatal rhesus macaques.	enterovirus 71 (ev71) is one of the main pathogens responsible for hand, foot, and mouth disease (hfmd). infection with ev71 can lead to severe clinical disease via extensive infections of either the respiratory or alimentary tracts in children. based on the previous pathological study of ev71 infections in neonatal rhesus macaques, our work using this animal model and an ev71 chimera that expresses enhanced green fluorescent protein (egfp-ev71) primarily explored where ev71 localizes and prolif ...	2017	28540257
pulmonary edema following central nervous system lesions induced by a non- mouse-adapted ev71 strain in neonatal balb/c mice.	enterovirus (ev) infection has been a serious health issue in asia-pacific region. it has been indicated that the occurrence of fatal hand foot and mouth disease (hfmd) cases following ev71 infection is mainly attributed to pulmonary edema. however, the development of pulmonary disorders after ev71 infection remains largely unknown. to establish an ev71-infected animal model and further explore the underlying association of central nervous system (cns) invasion with pulmonary edema, we isolated ...	2017	29282065
vp1 amino acid residue 145 of enterovirus 71 is a key residue for its receptor attachment and resistance to neutralizing antibody during cynomolgus monkey infection.	enterovirus 71 (ev71) is a causative agent of hand, foot, and mouth disease and sometimes causes severe or fatal neurological complications. the amino acid at vp1-145 determines the virological characteristics of ev71. viruses with glutamic acid (e) at vp1-145 (vp1-145e) are virulent in neonatal mice and transgenic mice expressing human scavenger receptor b2, whereas those with glutamine (q) or glycine (g) are not. however, the contribution of this variation to pathogenesis in humans is not full ...	2018	29848582
establishment and characterization of an oral gerbil model for a non-mouse-adapted enterovirus 71 strain.	enterovirus 71 (ev71) is one of the major pathogens causing hand, foot, and mouth disease (hfmd) with neurological and systemic complications worldwide, and it is mostly discovered in infants and young children. it is of great significance to establish suitable animal models of ev71 infection on research of distribution and pathogenesis of the virus. in this study, we established a successful infection of a non-mouse-adapted isolate of ev71 via oral route in 7-day-old mongolian gerbil (meriones ...	2018	30030018
enhanced neutralizing antibody response induced by inactivated enterovirus 71 in cynomolgus monkeys.	enterovirus 71 (ev71) is a major etiological agent of various public health issues, particularly in the asia-pacific region. ev71 causes hand-foot-and-mouth disease (hfmd) and is associated with serious neurological disorders in young children. a formalin-inactivated ev71 candidate vaccine (kcdc-hfmdv1-ev71) based on the c4 subgenotype was previously developed and confirmed to be a potential candidate vaccine for prevention of ev71 infection in mice. in this study, an inactivated ev71 vaccine wa ...	2018	30278051
high-throughput sequencing of putative novel micrornas in rhesus monkey peripheral blood mononuclear cells following ev71 and ca16 infection.	enterovirus 71 (ev71) and coxsackievirus a16 (ca16) remain the major pathogens in hand, foot, and mouth disease (hfmd) cases, but the mechanisms of the different pathogeneses that follow ev71 and ca16 infection remain largely unknown.	2018	30404089
preparation and identification of chicken egg yolk immunoglobulins against human enterovirus 71 for diagnosis of hand-foot-and-mouth disease.	human enterovirus 71 (ev71) is one of the major pathogens that causes hand-foot-and mouth disease, and there is an urgent need for rapid diagnosis of ev71 virus infection for early antiviral treatment. the aim of this study was to prepare chicken egg yolk immunoglobulin y (igy) for the diagnosis of enterovirus type 71 infection. the antibodies were raised by intramuscular immunization of laying hens with inactivated human ev71 and isolated from the egg yolk by multiple steps of polyethylene glyc ...	2019	30831098
enterovirus type 71-immunized chicken egg yolk immunoglobulin has cross antiviral activity against coxsackievirus a16 in vitro.	to exploit a cross passive immunotherapy for enterovirus-induced hand-foot-and-mouth disease (hfmd), the cross antiviral activity of a neutralizing antibody against enterovirus 71 (ev71) and coxsackievirus a16 (cva16) was investigated in vitro. white leghorn specific-pathogen-free chickens were immunized with ev71 antigens and a specific isolated immunoglobulin (igy) was prepared from the chicken egg yolk. igy was further purified and characterized by sds-page, elisa, western blotting and bidire ...	2019	31258670
study of integrated protective immunity induced in rhesus macaques by the intradermal administration of a bivalent ev71-ca16 inactivated vaccine.	enterovirus type 71 (ev71) and coxsackievirus a 16 (ca16) are recognized as the major pathogens responsible for human hand-foot-mouth disease. to develop a bivalent ev71-ca16 vaccine, rhesus macaques immunized with two doses of this vaccine via the intradermal route were challenged with ev71 or ca16, and their clinical symptoms, viral shedding, neutralizing antibodies, ifn-γ-specific elispots, and tissue viral load were examined longitudinally. specific immunity against ev71 and ca16 was observe ...	2020	31982260
safety and immunogenicity of an experimental live combination vaccine against enterovirus 71 and coxsackievirus a16 in rhesus monkeys.	enterovirus 71 (ev-a71) and coxsackievirus a16 (cv-a16) are the two most common pathogens causing hand, foot, and mouth disease (hfmd). previously, we obtained one candidate live attenuated strain each for ev-a71 and cv-a16; here, we evaluated the safety and immunogenicity of a combinedlive vaccine against ev-a71 and cv-a16 generated from these two candidate strains. rhesus monkeys were intramuscularly treated with a live combinationvaccine against both ev-a71 and cv-a16 or with either vaccine a ...	2020	32159429
establishment and transcriptomic features of an immortalized hepatic cell line of the chinese tree shrew.	the chinese tree shrew (tupaia belangeri chinesis) is a rising experimental animal and has been used for studying a variety of human diseases, such as metabolic and viral infectious diseases.	2020	32880691
preclinical safety assessment of a combined vaccine against hepatitis a virus and enterovirus 71.	since 2007, hepatitis a (hav) vaccination has been a part of the national immunization program of china. recognizing enterovirus 71 (ev71) as the most important pathogen in severe hand, foot and mouth disease, an inactivated ev71 vaccine was successfully marketed in 2015. based on the concept of one vaccine preventing two diseases and owing to similarities in vaccine preparation and the overlap of the eligible population, a combination of the inactivated hav vaccine and inactivated ev71 vaccine ...	2021	34088510