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the impact of high temperatures on vitis vinifera cv. semillon grapevine performance and berry ripening.the heat event that occurred in many parts of australia in 2009 was the worst on record for the past decade, with air temperatures exceeding 40(°)c for 14 days. our aim was to assess the impacts of this heat event on vine performance, including ripening, yield, and gas exchange of vitis vinifera cv. semillon grown in a riverina vineyard. to assess the affect of high temperatures on semillon grapevines, the vines were covered with a protective layer to reduce radiant heating and were compared wit ...201324348494
new stable qtls for berry weight do not colocalize with qtls for seed traits in cultivated grapevine (vitis vinifera l.).in grapevine, as in other fruit crops, fruit size and seed content are key components of yield and quality; however, very few quantitative trait loci (qtls) for berry weight and seed content (number, weight, and dry matter percentage) have been discovered so far. to identify new stable qtls for marker-assisted selection and candidate gene identification, we performed simultaneous qtl detection in four mapping populations (seeded or seedless) with various genetic backgrounds.201324350702
genome-wide analysis of respiratory burst oxidase homologs in grape (vitis vinifera l.).plant respiratory burst oxidase homolog (rboh) genes appear to play crucial roles in plant development, defense reactions and hormone signaling. in this study, a total of seven rboh genes from grape were identified and characterized. genomic structure and predicted protein sequence analysis indicated that the sequences of plant rboh genes are highly conserved. synteny analysis demonstrated that several vvrboh genes were found in corresponding syntenic blocks of arabidopsis, suggesting that these ...201324351809
functional diversification of grapevine myb5a and myb5b in the control of flavonoid biosynthesis in a petunia anthocyanin regulatory mutant.flavonoids play a key role in grapevine physiology and also contribute substantially to the quality of berries and wines. vvmyb5a and vvmyb5b are r2r3-myb transcription factors previously proposed to control the spatiotemporal expression of flavonoid structural genes during berry development. we investigated the functions of these two proteins in detail by heterologous expression in a petunia an2 mutant, which has negligible anthocyanin levels in the petals because it lacks the myb protein phan2 ...201424363289
interactions between ethylene and auxin are crucial to the control of grape (vitis vinifera l.) berry ripening.fruit development is controlled by plant hormones, but the role of hormone interactions during fruit ripening is poorly understood. interactions between ethylene and the auxin indole-3-acetic acid (iaa) are likely to be crucial during the ripening process, since both hormones have been shown to be implicated in the control of ripening in a range of different fruit species.201324364881
reproductive mode and fine-scale population genetic structure of grape phylloxera (daktulosphaira vitifoliae) in a viticultural area in california.grape phylloxera (daktulosphaira vitifoliae) is one of the world's most important viticultural pests. however, the reproductive mode, genetic structure and host adaptation of phylloxera in various viticultural environments remains unclear. we examined reproductive mode and genetic structure of phylloxera by analyzing microsatellite makers across the samples from four vineyard-sites in california.201324367928
the grape aquaporin vvsip1 transports water across the er membrane.water diffusion through biological membranes is facilitated by aquaporins, members of the widespread major intrinsic proteins (mips). in the present study, the localization, expression, and functional characterization of a small basic intrinsic protein (sip) from the grapevine were assessed. vvsip1 was expressed in leaves and berries from field-grown vines, and in leaves and stems from in vitro plantlets, but not in roots. when expressed in tobacco mesophyll cells and in saccharomyces cerevisiae ...201424376256
grapevine rootstock effects on scion sap phenolic levels, resistance to xylella fastidiosa infection, and progression of pierce's disease.the xylem-limited bacterium xylella fastidiosa (xf) causes pierce's disease (pd), an important disease of grapevine, vitis vinifera l. grapevine rootstocks were developed to provide increased resistance to root disease, but rootstock effects on cane and vine diseases remain unclear. grapevines that consisted of cabernet sauvignon or chardonnay grafted to 13 different rootstocks were inoculated with xf and evaluated for pd severity and xf titer after 6 months. a subset of six rootstock/scion comb ...201324376452
the dual diverse dynamic reversible effects of ankaferd blood stopper on epcr and pai-1 inside vascular endothelial cells with and without lps challenge.ankaferd blood stopper (abs) is comprised of a mixture of the plants thymus vulgaris, glycyrrhiza glabra, vitis vinifera, alpinia officinarum, and urtica dioica. abs is used as a topical hemostatic agent due to its antihemorrhagic effect, yet its hemostatic mechanism of action remains to be investigated. abs does not affect the levels of coagulation factors ii, v, vii, viii, ix, x, xi and xii. the aim of this study was to investigate the effects of abs on endothelium and immune response. as such ...201224385723
mode of action of a fatty acid-based natural product to control botrytis cinerea in grapes.to investigate the efficacy and mode of action of the fatty acid-based product foodcoat(®) (fc) against botrytis cinerea.201424387715
biodiversity study of wine yeasts belonging to the "terroir" of montepulciano d'abruzzo "colline teramane" revealed saccharomyces cerevisiae strains exhibiting atypical and unique 5.8s-its restriction patterns.the montepulciano d'abruzzo "colline teramane" premium wine docg is produced in the teramo province (abruzzo, italy). this region has a great tradition in winemaking and the wine is produced by a spontaneous fermentation so it could represent a reservoir of wine natural yeasts with important oenological features. the aim of this study was to characterize the yeast community of this wine grape growing region in order to create a saccharomyces cerevisiae bank, providing data on oenological propert ...201424387846
inheritance of downy mildew (plasmopara viticola) and anthracnose (sphaceloma ampelinum) resistance in grapevines.downy mildew (plasmopara viticola) and anthracnose (sphaceloma ampelinum) are two of the major diseases of most grapevine (vitis vinifera l.) cultivars grown in thailand. therefore, breeding grapevines for improved downy mildew and anthracnose resistance is crucial. factorial crosses were made between three downy mildew and/or anthracnose resistant lines ('ny88.0517.01', 'ny65.0550.04', and 'ny65.0551.05'; male parents) and two or three susceptible cultivars of v. vinifera ('black queen', 'carol ...201324391016
transcriptome analysis of houttuynia cordata thunb. by illumina paired-end rna sequencing and ssr marker discovery.houttuynia cordata thunb. is an important traditional medical herb in china and other asian countries, with high medicinal and economic value. however, a lack of available genomic information has become a limitation for research on this species. thus, we carried out high-throughput transcriptomic sequencing of h. cordata to generate an enormous transcriptome sequence dataset for gene discovery and molecular marker development.201424392108
geochemical behaviour of rare earths in vitis vinifera grafted onto different rootstocks and growing on several soils.the geochemical behaviour of lanthanides and yttrium (rare earth elements, rees) has been investigated mainly in geological systems where these elements represent the best proxies of processes involving the occurrence of an interface between different media. this behaviour is assessed according to features recorded in sequences of ree concentrations along the ree series normalised with respect to a reference material. in this study, the geochemical behaviour of ree was investigated in different ...201424394369
morphological and phylogenetic identification of botrytis sinoviticola, a novel cryptic species causing gray mold disease of table grapes (vitis vinifera) in china.seventy-five isolates of botrytis collected from table grapes (vitis vinifera) with gray mold symptoms in china were identified based on morpho-cultural characteristics on potato dextrose agar (20 c) and/or phylogenetic analysis using the sequences of three nuclear genes (g3pdh, hsp60, rpb2). isolates of different species of botrytis were compared with fenhexamid sensitivity, bc-hch gene-rflp haplotyping and pathogenicity to v. vinifera. the 75 isolates comprise two species, b. cinerea (63 isola ...201424396103
whole genome comparison between table and wine grapes reveals a comprehensive catalog of structural variants.grapevine (vitis vinifera l.) is the most important mediterranean fruit crop, used to produce both wine and spirits as well as table grape and raisins. wine and table grape cultivars represent two divergent germplasm pools with different origins and domestication history, as well as differential characteristics for berry size, cluster architecture and berry chemical profile, among others. 'sultanina' plays a pivotal role in modern table grape breeding providing the main source of seedlessness. t ...201424397443
extraction process optimization of polyphenols from indian citrus sinensis - as novel antiglycative agents in the management of diabetes mellitus.diabetes mellitus is a chronic metabolic disorder characterized by increased blood glucose level. it has become an epidemic disease in the 21st century where, india leads the world with largest number of diabetic subjects. non-enzymatic glycosylation (glycation) is severe form of diabetes, occurs between reducing sugar and proteins which results in the formation of advanced glycation end products (ages) that leads to the other complicated secondary disorders. in this context, mangifera indica (m ...201424397983
gene expression in vessel-associated cells upon xylem embolism repair in vitis vinifera l. petioles.in this work, the involvement of vessel-associated cells in embolism recovery was investigated by studying leaf petiole hydraulics and expression profiles of aquaporins and genes related to sugar metabolism. two different stress treatments were imposed onto grapevines to induce xylem embolism: one involved a pressure collar applied to the stems, while the other consisted of water deprivation (drought). embolism formation and repair were monitored during stress application and release (recovery). ...201424402563
the gene family of dehydration responsive element-binding transcription factors in grape (vitis vinifera): genome-wide identification and analysis, expression profiles, and involvement in abiotic stress resistance.the dehydration responsive element-binding (dreb) proteins play a critical role in plant development and abiotic stress-mediated gene expression. therefore, they represent one of the most attractive regulons for breeding programs. however, no comprehensive summary of grapevine dreb family genes is available. during this study, 38 vvdreb members were identified from the entire grapevine genome and its expression sequence tag assembly. these were organized into the same subgroups, a1 through a6, a ...201424402876
α-glucosidase from grape berries: partial purification and characterization.α-glucosidase (α-d-glucoside glucohydrolase ec 3.2.1.20) was purified approximately 30-fold from grape berries (vitis vinifera var. riesling). besides maltose the enzyme preparation hydrolyzes to a lesser extent maltotriose, isomaltose, and starch. it has a ph optimum of 5.1 and a molecular weight of about 100,000. tris, glycerol, several mono-and disaccharides were tested as inhibitors. the kinetic behavior of ribose, fructose, cellobiose, sucrose, turanose, methylglucopyranoside, tris, and gly ...197824408102
generation of expressed sequence tags under cadmium stress for gene discovery and development of molecular markers in chickpea.chickpea is the world's third most important legume crop and belongs to fabaceae family but suffered from severe yield loss due to various biotic and abiotic stresses. development of modern genomic tools such as molecular markers and identification of resistant genes associated with these stresses facilitate improvement in chickpea breeding towards abiotic stress tolerance. in this study, 1597 high-quality expressed sequence tags (ests) were generated from a cdna library of variety pusa 1105 roo ...201424414095
morphological variability in leaves and molecular characterization of novel table grape candidate cultivars (vitis vinifera l.).the present work report the characterization of twenty-one table grapes candidate cultivars plus five registered ones included as reference, by means of 47 ampelographic traits, 23 ampelometric measurements and six microsatellite loci. the final goal of the research was to analyse the possibility of reducing the number of morphological and molecular tools required for a precise and effective description of a grape genotype or cultivar. this would be of great help for future biodiversity descript ...201424415447
the effect of fruit-removal on cytokinins and gibberellin-like substances in grape leaves.removal of fruit from potted cuttings of vitis vinifera l. increased the concentration of a cytokininglucoside in leaf tissue extracts and decreased the level of extractable gibberellin-like substances. the glucoside (of zeatin riboside) is not present in xylem exudate of v. vinifera l., and appears to be synthesized in the leaves. berry extracts contain zeatin-riboside and smaller amounts of cytokinin-glucoside. the changes in the level of these hormones are discussed in relation to previous re ...197724420222
ultrastructural studies of the binding of concanavalin a to the plasmalemma of higher plant protoplasts.the binding of concanavalin a to the plasmalemma of higher plants has been studied using protoplasts of two species. the lectin aggregates both tobacco (nicotiana tabacum l.) leaf protoplasts and protoplasts prepared from a suspension cell culture of grapevine (vitis vinifera l.). differences in lectin binding have been investigated using concanavalin a conjugated to ferritin or bound to colloidal gold. tobacco protoplasts exhibit continuous and saturated labelling of the plasmalemma surface wit ...197624424545
scanning electron microscopy of cell wall formation around isolated plant protoplasts.the process of cell wall regeneration around two species of higher plant protoplasts has been studied using reflection scanning electron microscopy. the first stage in the process is the formation of short fibres from randomly spaced centres. with protoplasts of tobacco leaf (nicotiana tabacum l., cv white burley) these fibres then elongate and interlace apparently at random to give rise to a matted continuous layer of wall. protoplasts of a suspension culture of grapevine cells (vitis vinifera ...197624424768
[after-effects in untreated progenies from chickens treated with the products from resistant interspecific grape-vine hybrids].feeding hens and cocks for 6-8 weeks with the products - wines and juices - from ± resistant interspecific grapevine varieties (f1, f2r), i.e. from crosses between sensitive types of vitis vinifera and ± resistant american wild types, led to physiological changes of the treated animals. these changes became evident by more than 40% of malformations, especially of legs and plumage, among the individuals of their untreated f1 progeny. these effects proved to be reproducable.among the f2-individual ...197124430524
factors affecting skin tannin extractability in ripening grapes.the acetone-extractable (70% v/v) skin tannin content of vitis vinifera l. cv. cabernet sauvignon grapes was found to increase during late-stage ripening. conversely, skin tannin content determined following ethanol extraction (10, 20, and 50% v/v) did not consistently reflect this trend. the results indicated that a fraction of tannin became less extractable in aqueous ethanol during ripening. skin cell walls were observed to become more porous during ripening, which may facilitate the sequeste ...201424432763
efficacy of food supplement to improve metabolic syndrome parameters in patients affected by moderate to severe psoriasis during anti-tnfα treatment.psoriasis is a systemic inflammatory immune-mediated skin disease. recently a relationship with metabolic syndrome in terms of psoriasis severity and response to therapy was observed.201324442048
effect of viticulture practices on concentration of polyphenolic compounds and total antioxidant capacity of southern italy red wines.this study aims to assess the effect of three wine grape varieties, three training systems and two bud loads on the total antioxidant capacity (tac) and polyphenolic composition of southern italy red wines produced, during two vintages. overall, primitivo, malvasia nera of brindisi-lecce and montepulciano as grape varieties, single guyot (sg), single spur pruned low cordon (slc) and single spur pruned high wire cordon (hslc) as training systems, 8 and 12 buds/plant as bud loads were compared. si ...201424444963
the phenolic chemistry and spectrochemistry of red sweet wine-making and oak-aging.a natural sweet wine (nsw) was made with dried grapes from vitis vinifera l. cv garnacha tintorera. a fortified sweet wine (fsw) was also obtained: the maceration-alcoholic fermentation of garnacha tintorera must was stopped by addition of ethanol 96% (v/v). uv/vis spectrophotometry and hplc/dad-esi/ms were applied to determine, respectively, the evolution of colour and phenolic compounds in garnacha tintorera based-sweet wines during aging. in sweet wines, aging decreased a(∗) (red/green), colo ...201424444970
interactions between grape skin cell wall material and commercial enological tannins. practical implications.commercial enological tannins were used to investigate the role that cell wall material plays in proanthocyanidin adsorption. insoluble cell wall material, prepared from the skin of vitis vinifera l. cv. monastrell berries, was combined with solutions containing six different commercial enological tannins (proanthocyanidin-type tannins). analysis of the proanthocyanidins in the solution, after fining with cell wall material, using phloroglucinolysis and size exclusion chromatography, provided qu ...201424444975
unravelling the diversity of grapevine microbiome.vitis vinifera is one of the most widely cultivated fruit crops with a great economic impact on the global industry. as a plant, it is naturally colonised by a wide variety of both prokaryotic and eukaryotic microorganisms that interact with grapevine, having either beneficial or phytopathogenic effects, who play a major role in fruit yield, grape quality and, ultimately, in the evolution of grape fermentation and wine production. therefore, the objective of this study was to extensively charact ...201424454903
identification and bioinformatic analysis of signal responsive/calmodulin-binding transcription activators gene models in vitis vinifera.in this study, 10 grapevine (vitis vinifera) sr/camta (signal responsive/calmodulin-binding transcription activators) gene models were identified from three grapevine genome protein datasets. they belong to four gene groups: vvcamta1, vvcamta3, vvcamta4 and vvcamta5, which were located on chromosome 5, 7_random, 1 and 5, respectively. alternative splicing could explain the multiple gene models in one gene group. subcellular localization using the wolf tool showed that most of the vvcamtas were l ...201424458826
low-dose irradiation can be used as a phytosanitary treatment for fresh table grapes.grapes (vitis vinifera var. sugraone and vitis labrusca var. crimson seedless) were treated with 400, 600, and 800 gy and the effects on physicochemical factors were measured alongside sensory testing during 3 wk of storage. significant changes in texture and color with irradiation and age were measured but little visual difference was seen between control and irradiated grapes. however, age had a greater effect on firmness than irradiation for sugraone grapes. irradiation did not significantly ...201424460773
a novel high resolution ms approach for the screening of 4-hydroxy-trans-2-nonenal sequestering agents.an in vitro high resolution mass spectrometry (ms) method was set-up to test the ability of compounds, mixtures and extracts to inhibit protein carbonylation induced by reactive carbonyl species (rcs). the method consists of incubating the protein target (ubiquitin) with 4-hydroxy-trans-2-nonenal (hne) in the presence and absence of the tested compound. after 24h of incubation, the reaction is stopped and the protein is analyzed by high-resolution ms. the extent of protein carbonylation is deter ...201424463041
is stored malate the quantitatively most important substrate utilised by respiration and ethanolic fermentation in grape berry pericarp during ripening?a widely held view is that in grape pericarp glycolysis is inhibited during ripening, and that stored malate rather than sugars become the major substrate for respiration. in this study we determined what contribution stored malate could make to the substrate requirements of respiration and ethanolic fermentation in the pericarp of cabernet sauvignon berries during ripening. at a number of time points through development the amount of malate in the pericarp was measured. the change in malate con ...201424463535
pseudococcus maritimus (hemiptera: pseudococcidae) and parthenolecanium corni (hemiptera: coccidae) are capable of transmitting grapevine leafroll-associated virus 3 between vitis x labruscana and vitis vinifera.the grape mealybug, pseudococcus maritimus (ehrhorn), and european fruit lecanium scale, parthenolecanium corni (bouché), are the predominant species of coccoidea in washington state vineyards. the grape mealybug has been established as a vector of grapevine leafroll-associated virus 3 (glrav-3) between wine grape (vitis vinifera l.) cultivars, elevating its pest status. the objective of this study was to determine if glrav-3 could be transmitted between vitis x labruscana l. and v. vinifera by ...201324468559
neuroprotective role of hydroalcoholic extract of vitis vinifera against aluminium-induced oxidative stress in rat brain.the present study was designed to examine the protective potential of hydroalcoholic extract of vitis vinifera in ameliorating the alterations induced by aluminium (al) on behavioural and neurochemical indices. al was given orally (100mg/kg b.wt./day) whereas v. vinifera extract was administered through diet (400mg/kg, p.o.) to rats for a total duration of 45 days. passive avoidance and open field tests revealed significant alterations in the short-term memory and cognitive behaviour in rats tre ...201424486960
the grapevine flagellin receptor vvfls2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium burkholderia phytofirmans and plant pathogenic bacteria.• the role of flagellin perception in the context of plant beneficial bacteria still remains unclear. here, we characterized the flagellin sensing system flg22-flagellin sensing 2 (fls2) in grapevine, and analyzed the flagellin perception in the interaction with the endophytic plant growth-promoting rhizobacterium (pgpr) burkholderia phytofirmans. • the functionality of the grapevine fls2 receptor, vvfls2, was demonstrated by complementation assays in the arabidopsis thaliana fls2 mutant, which ...201424491115
sample preparation issues in nmr-based plant metabolomics: optimisation for vitis wood samples.nuclear magnetic resonance (nmr) is one of the most commonly used analytical techniques in plant metabolomics. although this technique is very reproducible and simple to implement, sample preparation procedures have a great impact on the quality of the metabolomics data.201424497327
impacts of standard wine-making process on the survival of lobesia botrana larvae (lepidoptera: tortricidae) in infested grape clusters.to determine the risk winery waste poses for the spread of lobesia botrana (denis & schiffermüller) (lepidoptera: tortricidae) in california, we evaluated the survival of larvae in artificially infested grape clusters (vitis vinifera l.) processed for wine making. the trial consisted of five treatments: whole cluster pressing to 1 bar (100,000 pa); whole cluster pressing to 2 bars (200,000 pa); destemming and berry pressing to 1 bar; destemming and berry pressing to 2 bars; and control. each tre ...201324498733
anticarcinogenic activity of polyphenolic extracts from grape stems against breast, colon, renal and thyroid cancer cells.a major part of the wineries' wastes is composed of grape stems which are discarded mainly in open fields and cause environmental problems due mainly to their high polyphenolic content. the grape stem extracts' use as a source of high added value polyphenols presents great interest because this combines a profitable venture with environmental protection close to wine-producing zones. in the present study, at first, the total polyphenolic content (tpc) and the polyphenolic composition of grape st ...201424508987
evolution and expression analysis of the grape (vitis vinifera l.) wrky gene family.wrky proteins comprise a large family of transcription factors that play important roles in plant defence regulatory networks, including responses to various biotic and abiotic stresses. to date, no large-scale study of wrky genes has been undertaken in grape (vitis vinifera l.). in this study, a total of 59 putative grape wrky genes (vvwrky) were identified and renamed on the basis of their respective chromosome distribution. a multiple sequence alignment analysis using all predicted grape wrky ...201424510937
the sulfated laminarin triggers a stress transcriptome before priming the sa- and ros-dependent defenses during grapevine's induced resistance against plasmopara viticola.grapevine (vitis vinifera) is susceptible to many pathogens which cause significant losses to viticulture worldwide. chemical control is available, but agro-ecological concerns have raised interest in alternative methods, especially in triggering plant immunity by elicitor treatments. the β-glucan laminarin (lam) and its sulfated derivative (ps3) have been previously demonstrated to induce resistance in grapevine against downy mildew (plasmopara viticola). however, if lam elicits classical grape ...201424516597
identification, classification and differential expression of oleosin genes in tung tree (vernicia fordii).triacylglycerols (tag) are the major molecules of energy storage in eukaryotes. tag are packed in subcellular structures called oil bodies or lipid droplets. oleosins (ole) are the major proteins in plant oil bodies. multiple isoforms of ole are present in plants such as tung tree (vernicia fordii), whose seeds are rich in novel tag with a wide range of industrial applications. the objectives of this study were to identify ole genes, classify ole proteins and analyze ole gene expression in tung ...201424516650
relationships between harvest time and wine composition in vitis vinifera l. cv. cabernet sauvignon 2. wine sensory properties and consumer preference.a series of five vitis vinifera l. cv cabernet sauvignon wines were produced from sequentially-harvested grape parcels, with alcohol concentrations between 12% v/v and 15.5% v/v. a multidisciplinary approach, combining sensory analysis, consumer testing and detailed chemical analysis was used to better define the relationship between grape maturity, wine composition and sensory quality. the sensory attribute ratings for dark fruit, hotness and viscosity increased in wines produced from riper gra ...201424518320
abiotic stresses differentially affect the expression of o-methyltransferase genes related to methoxypyrazine biosynthesis in seeded and parthenocarpic fruits of vitis vinifera (l.).mps (3-alkyl-2-methoxypyrazines) are grape-derived aroma compounds that are associated with detrimental herbaceous flavours in some wines. it is well known that several viticultural and environmental parameters can modulate mp concentrations in grapes, although comprehensive molecular studies have not been conducted in this field. although the biosynthesis pathway of mps has not been fully elucidated, four vitis vinifera o-methyltransferase genes (vvomt1-4) have been related to be involved in mp ...201424518323
viniphenol a, a complex resveratrol hexamer from vitis vinifera stalks: structural elucidation and protective effects against amyloid-β-induced toxicity in pc12 cells.stilbenes have received much attention during the last two decades following the discovery of resveratrol in wine. since then, there have been a growing number of papers reporting various biological activities of naturally occurring stilbenes. the aim of this study was to determine new minor stilbenes from vitis vinifera stalks. purification of these compounds was achieved by means of centrifugal partition chromatography, a versatile separation technique that does not require a solid stationary ...201424521157
effect of ccc on the growth of roots of vitis vinifera l.when plants of vitis vinifera l. were grown in solution cultures containing (2-chloroethyl)trimethylammonium chloride (ccc), roots became shorter and thicker than those of untreated control plants. the effect of ccc on vine roots could be counteracted by gibberellic acid simultaneously applied either to the solution cultures or to aerial organs of the plant.196724522506
assessment of cymbopogon citratus (dc.) stapf essential oil as herbal preservatives based on antifungal, antiaflatoxin, and antiochratoxin activities and in vivo efficacy during storage.thirty-five randomly collected samples of stored table grapes (vitis vinifera l.) from different markets of gorakhpur city, uttar pradesh, india, revealed occurrence of 11 types of fungi. of which, aspergillus flavus, aspergillus niger, and aspergillus ochraceus were dominant causing severe decay of grapes with 58%, 52%, and 67% incidence, respectively. on screening of 15 essential oils at 0.33 μl/ml, cymbopogon citratus oil caused 100% mycelial inhibition against aforesaid dominant fungi. oil w ...201424547889
effect of acoustic frequency and power density on the aqueous ultrasonic-assisted extraction of grape pomace (vitis vinifera l.) - a response surface approach.aqueous ultrasound-assisted extraction (uae) of grape pomace was investigated by response surface methodology (rsm) to evaluate the effect of acoustic frequency (40, 80, 120khz), ultrasonic power density (50, 100, 150w/l) and extraction time (5, 15, 25min) on total phenolics, total flavonols and antioxidant capacity. all the process variables showed a significant effect on the aqueous uae of grape pomace (p<0.05). the box-behnken design (bbd) generated satisfactory mathematical models which accu ...201424548543
gibberellin-like substances in root exudate of vitis vinifera.the levels of gibberellin (ga)-like activity in the root exudate of two seedless varieties of vitis vinifera were examined by the barley endosperm assay, and compared with levels determined for other parts of the plant. that activity was due to ga-like substances was confirmed with dwarf-5 corn.when acidic, ethyl acetate soluble ga-like substances from sap and leaf extracts were chromatogrammed on thin layers of silica gel in chloroform/ethyl acetate/formic acid (50:50:1), activity moved to the ...196724549951
interkingdom transfer of the acne-causing agent, propionibacterium acnes, from human to grapevine.here, we report the surprising and, to our knowledge, unique example of horizontal interkingdom transfer of a human opportunistic pathogen (propionibacterium acnes) to a crop plant (the domesticated grapevine vitis vinifera l.). humans, like most organisms, have established a long-lasting cohabitation with a variety of microbes, including pathogens and gut-associated bacteria. studies which have investigated the dynamics of such associations revealed numerous cases of bacterial host switches fro ...201424554779
biochemical and biological assessment of the inhibitory potency of extracts from vinification byproducts of vitis vinifera extracts against glycogen phosphorylase.the inhibitory potency of thirteen polyphenolic extracts obtained from vinification byproducts of greek varieties of vitis vinifera against glycogen phosphorylase (gp) has been studied by kinetic experiments. gp is an enzyme involved in glucose homeostasis and a molecular target for the discovery of new hypoglycemic agents. studies have shown that all extracts display significant inhibitory potency for gp in vitro with ic50 values in the range of low μg/ml. x-ray crystallographic analysis of gp ...201424556570
heritability and identification of qtls and underlying candidate genes associated with the architecture of the grapevine cluster (vitis vinifera l.).we have identified 19 qtls for rachis architecture, a key and complex trait for grapevine production. fifty out of 1,173 genes underlying these qtls are candidates to be further explored. in the table grape industry, the rachis architecture has economic and management implications. therefore, understanding the genetics of this trait is key for its breeding. the aim of this work was to identify genetic determinants of traits associated with the cluster architecture. characterisations of eight tra ...201424556794
[effects of different drip irrigation modes on root distribution of wine grape 'cabernet sauvignon' in desert area of northwest china].to study the effects of different drip irrigation modes on the wine grape root distribution is the basis of formulating fertilization, irrigation, and over-wintering management practices for wine grape. taking the wine grape "cabernet sauvignon" as test material, this paper studied the effects of different water-saving irrigation modes (drip irrigation under straw mulching, drip irrigation under plastic mulching, double-tube drip irrigation, and single-tube drip irrigation) on the root distribut ...201324564135
genomic analysis of nac transcription factors in banana (musa acuminata) and definition of nac orthologous groups for monocots and dicots.identifying the molecular mechanisms underlying tolerance to abiotic stresses is important in crop breeding. a comprehensive understanding of the gene families associated with drought tolerance is therefore highly relevant. nac transcription factors form a large plant-specific gene family involved in the regulation of tissue development and responses to biotic and abiotic stresses. the main goal of this study was to set up a framework of orthologous groups determined by an expert sequence compar ...201424570169
dentin biomodification potential depends on polyphenol source.although proanthocyanidins (pacs) modify dentin, the effectiveness of different pac sources and the correlation with their specific chemical composition are still unknown. this study describes the chemical profiling of natural pac-rich extracts from 7 plants using ultra high pressure/performance liquid chromatography (uhplc) to determine the overall composition of these extracts and, in parallel, comprehensively evaluate their effect on dentin properties. the total polyphenol content of the extr ...201424574140
biomolecule-mediated synthesis of selenium nanoparticles using dried vitis vinifera (raisin) extract.biomolecule-mediated nanoparticle synthesis has recently the gained attention of researchers due to its ecofriendly and non-toxic nature. metabolites from plant extracts represent a better alternative to chemical methods to fulfill the growing demand for non-hazardous nanoparticle synthesis routes. selenium and its nanoparticles have an extensive range of applications. thus, biofabrication of selenium nanoparticles can be potentially useful in various fields. this study reports a green approach ...201424583881
the tvv1 retrotransposon family is conserved between plant genomes separated by over 100 million years.combining several different approaches, we have examined the structure, variability, and distribution of tvv1 retrotransposons. tvv1 is an unusual example of a low-copy retrotransposon metapopulation dispersed unevenly among very distant species and is promising for the development of molecular markers. retrotransposons are ubiquitous throughout the genomes of the vascular plants, but individual retrotransposon families tend to be confined to the level of plant genus or at most family. this rest ...201424590356
effects of chitosan on the protein profile of grape cell culture subcellular fractions.grapevine is a large source of healthy polyphenols for human diet, and red table-grapes and wines are the main source of stilbenes. these compounds are important both in the plant defence system and for human health. in the present study, vitis vinifera cv. barbera cell cultures were treated with 50 μg/ml chitosan and proteomic analyses on soluble and membrane subcellular fractions were performed against suitable controls. three soluble stilbene synthase protein spots, four stilbene synthase spo ...201424590893
the proteins of the grape (vitis vinifera l.) seed endosperm: fractionation and identification of the major components.in the present study, grape (vitis vinifera l.) seed endosperm proteins were characterized after sequential fractionation, according to a modified osborne procedure. the salt-soluble fraction (albumins and globulins) comprised the majority (58.4%) of the total extracted protein. the protein fractions analysed by sds-page showed similar bands, indicating different solubility of the same protein components. sds-page in non-reducing and reducing conditions revealed the polypeptide composition of th ...201424594165
influence of post-pruning storage on stilbenoid levels in vitis vinifera l. canes.increasing evidence for the health benefits of e-resveratrol has triggered interest in stilbenoids in grapes, wine and by-products. less attention has been paid to stilbenoid levels in viticulture residues. however, grape canes are a promising source of stilbenoids and have economic potential because they are a source of high-value phytochemicals. the objective of this research was to determine the effect of post-pruning storage on stilbenoid levels in grape canes. in most samples, the predomina ...201424594183
it's time to pop a cork on champagne's proteome!champagne is a world-renowned french sparkling wine, which undergoes many steps (fermentation, aging …) for its elaboration. various compounds might evolve during this winemaking process and thus modify its final quality. here, we report the first proteome analysis of two standard commercial champagne wines, using the powerful combinatorial peptide ligand library (cpll) technique. indeed, wine proteins are present in small amounts but they are key compounds, likely to impact on both foam quality ...201424594285
relationships between stomatal behavior, xylem vulnerability to cavitation and leaf water relations in two cultivars of vitis vinifera.current understanding of physiological mechanisms governing stomatal behavior under water stress conditions is still incomplete and controversial. it has been proposed that coordination of stomatal kinetics with xylem vulnerability to cavitation [vulnerability curve (vc)] leads to different levels of isohydry/anisohydry in different plant species/cultivars. in this study, this hypothesis is tested in vitis vinifera cultivars displaying contrasting stomatal behavior under drought stress. the cv m ...201424597791
[incidence and distribution of the genus armillaria in vineyards of the five protected designation of origin wines in galicia (northwestern spain)].the genus armillaria, specifically armillaria mellea, is an important phytopathological problem in the wine sector in galicia (nw spain), having caused yield reductions in vineyards for the last 15 years. the fungus attacks the root system, resulting in a decrease in vigour, and eventually in the death of the plant. up to now, there is no chemical or biological method really effective against the pathogen once it has infected the plant.201524603128
effects of vine water status on dimethyl sulfur potential, ammonium, and amino acid contents in grenache noir grapes (vitis vinifera).we studied the effect of vine water status on the dimethyl sulfur potential (dmsp), ammonium, and amino acid contents of the berry during the maturation of grenache noir grapes. water deficit increased the accumulation of amino acids in berries and favored yeast assimilable amino nitrogen. similarly, ammonium content was higher in berries from vines subjected to moderate water deficit. dmsp content followed the same trend as yeast assimilable amino acid content, with higher concentrations observ ...201424611597
tracing phenolic metabolism in vitis vinifera berries with 13c6-phenylalanine: implication of an unidentified intermediate reservoir.understanding the regulation of phenolic compounds in agricultural products has been a topic of great interest. in v. vinifera berries, phenolics are responsible for important sensory and functional characteristics. to elucidate the ripening profile of phenolic compounds in cabernet sauvignon berries, the stable-isotope tracer l-phenyl-(13)c6-alanine (phe(13)) was incorporated in situ, and the development of labeled and unlabeled phenolics was tracked in the vineyard at different stages of matur ...201424611798
hannaella pagnoccae sp. nov., a tremellaceous yeast species isolated from plants and soil.several independent surveys of yeasts associated with different plant materials and soil led to the proposal of a novel yeast species belonging to the tremellales clade (agaricomycotina, basidiomycota). analysis of the sequences of the d1/d2 domains and internal transcribed spacer region of the large subunit of the rrna gene suggested affinity to a phylogenetic lineage that includes hannaella coprosmaensis, hannaella oryzae and hannaella sinensis. thirty-two isolates were obtained from different ...201424614844
study on folate binding domain of dihydrofolate reductase in different plant species and human beings.data base (ncbi and tigr) searches are made to retrieve protein sequences of different plant species namely medicago truncatula, pisum sativum, ricinus communis, arabidopsis thaliana, vitis vinifera, glycine max, daucus carota, oryza sativa japonica group, arabidopsis lyrata subsp. lyrata, brachypodium distachyon, oryza sativa indica group, zea mays and careful alignment of derived sequences shows 95% or higher identity. similarly, dhfr sequence of human being is also retrieved from ncbi. a phyl ...201424616563
the first insight into the metabolite profiling of grapes from three vitis vinifera l. cultivars of two controlled appellation (doc) regions.the characterization of the metabolites accumulated in the grapes of specific cultivars grown in different climates is of particular importance for viticulturists and enologists. in the present study, the metabolite profiling of grapes from the cultivars, alvarinho, arinto and padeiro de basto, of two portuguese controlled denomination of origin (doc) regions (vinho verde and lisboa) was investigated by gas chromatography-coupled time-of-flight mass spectrometry (gc-tof-ms) and an amino acid ana ...201424619195
influence of magnesium on copper phytotoxicity to and accumulation and translocation in grapevines.the phytotoxic effects of excess copper (cu) on grapevines (vitis vinifera l. var. kyoho) were examined, both from macroscopic and microscopic perspectives, by using a fifteen-day hydroponic experiments. the influence of magnesium (mg) on cu phytotoxicity to, and accumulation and translocation in grapevines was also observed. for phytotoxicity effect, results showed that a relative low median growth inhibition level of cu was found for grapevine roots (0.809-3.671μm). moreover, cu toxicity was s ...201424632121
evaluation of five protocols for dna extraction from leaves of malus sieversii, vitis vinifera, and armeniaca vulgaris.leaves of malus sieversii, vitis vinifera, and armeniaca vulgaris contain substantial amounts of secondary metabolites, which limit the high-quality dna extraction performance. in this study, five extraction protocols were compared for their ability to produce good quality dna from fresh and dried (with silica gel) leaves of these species. the modified protocol of dellaporta et al., using polyvinylpyrrolidone to bind the phenolic compounds and a high molar concentration of potassium acetate to i ...201424634185
transcriptional analysis of tendril and inflorescence development in grapevine (vitis vinifera l.).in grapevine (vitis vinifera l.), the lateral meristem can give rise to either tendrils or inflorescences which are determined organs. to get insights into the processes of tendril and inflorescence development, we characterized the transcriptional variation taking place in both organs. the results of the global transcriptional analyses along tendril and inflorescence development suggested that these two homologous organs initially share a common transcriptional program related to cell prolifera ...201424637773
pyrosequencing reveals regional differences in fruit-associated fungal communities.we know relatively little of the distribution of microbial communities generally. significant work has examined a range of bacterial communities, but the distribution of microbial eukaryotes is less well characterized. humans have an ancient association with grape vines (vitis vinifera) and have been making wine since the dawn of civilization, and fungi drive this natural process. while the molecular biology of certain fungi naturally associated with vines and wines is well characterized, comple ...201424650123
characterization of grape (vitis vinifera l.) berry sunburn symptoms by reflectance.sunburn can affect grape quality both for chemical modifications and by visual impact of the browning. optical properties of 17 white grape accessions were investigated in the visible region using a noninvasive instrument. reflectance spectra were obtained using a jaz system spectrometer. browning was induced by exposing grape bunches to direct sunlight at 12:30 p.m. for 5 h. during the experiment, the global solar radiation ranged from 2.6 to 2.7 mj m-2 h-1 and the air temperature from 24.3 to ...201424650184
circadian oscillatory transcriptional programs in grapevine ripening fruits.temperature and solar radiation influence vitis vinifera l. berry ripening. both environmental conditions fluctuate cyclically on a daily period basis and the strength of this fluctuation affects grape ripening too. additionally, a molecular circadian clock regulates daily cyclic expression in a large proportion of the plant transcriptome modulating multiple developmental processes in diverse plant organs and developmental phases. circadian cycling of fruit transcriptomes has not been characteri ...201424666982
metabolic and transcript analysis of the flavonoid pathway in diseased and recovered nebbiolo and barbera grapevines (vitis vinifera l.) following infection by flavescence dorée phytoplasma.flavescence dorée phytoplasma (fdp) infections seriously affect production and survival of grapevine. we analysed the changes in the flavonoid pathway occurring in two red cultivars, the highly susceptible barbera and the less susceptible nebbiolo, following fdp infection. a combination of metabolic and transcript analyses was used to quantify flavonoid compounds and expression of a set of genes involved in their biosynthesis. quantification of anthocyanins, flavonols, proanthocyanidins and rela ...201424689527
is stomatal conductance optimized over both time and space in plant crowns? a field test in grapevine (vitis vinifera).crown carbon gain is maximized for a given total water loss if stomatal conductance (gs ) varies such that the marginal carbon product of water (∂a/∂e) remains invariant both over time and among leaves in a plant crown, provided the curvature of assimilation rate (a) versus transpiration rate (e) is negative. we tested this prediction across distinct crown positions in situ for the first time by parameterizing a biophysical model across 14 positions in four grapevine crowns (vitis vinifera), com ...201424689930
copper homeostasis in grapevine: functional characterization of the vitis vinifera copper transporter 1.the vitis vinifera copper transporter 1 is capable of self-interaction and mediates intracellular copper transport. an understanding of copper homeostasis in grapevine (vitis vinifera l.) is particularly relevant to viticulture in which copper-based fungicides are intensively used. in the present study, the vitis vinifera copper transporter 1 (vvctr1), belonging to the ctr family of copper transporters, was cloned and functionally characterized. amino acid sequence analysis showed that vvctr1 mo ...201424691572
the onset of grapevine berry ripening is characterized by ros accumulation and lipoxygenase-mediated membrane peroxidation in the skin.the ripening of fleshy fruits is a complex developmental program characterized by extensive transcriptomic and metabolic remodeling in the pericarp tissues (pulp and skin) making unripe green fruits soft, tasteful and colored. the onset of ripening is regulated by a plethora of endogenous signals tuned to external stimuli. in grapevine and tomato, which are classified as non-climacteric and climacteric species respectively, the accumulation of hydrogen peroxide (h2o2) and extensive modulation of ...201424693871
vvmate1 and vvmate2 encode putative proanthocyanidin transporters expressed during berry development in vitis vinifera l.vvmate1 and vvmate2 encode putative pa transporters expressed during seed development in grapevine. the subcellular localization of these mate proteins suggests different routes for the intracellular transport of pas. proanthocyanidins (pas), also called condensed tannins, protect plants against herbivores and are important quality components of many fruits. pas biosynthesis is part of the flavonoid pathway that also produces anthocyanins and flavonols. in grape fruits, pas are present in seeds ...201424700246
detection of new genetic profiles and allelic variants in improperly classified grapevine accessions.thirty-seven grapevine accessions, collected in central italy, were characterized by morphological and genetic analysis, according to guidelines developed by european union programs of grapevine research and standardization. traditional denominations of some sampled varieties were revealed to be incorrect; moreover, 10 synonymies and 12 homonymies were recognized. ampelographic and ampelometric measurements of leaf characters were performed. these data generated a phenotypic similarity matrix an ...201424702068
genome-wide identification, evolution and expression analysis of the grape (vitis vinifera l.) zinc finger-homeodomain gene family.plant zinc finger-homeodomain (zhd) genes encode a family of transcription factors that have been demonstrated to play an important role in the regulation of plant growth and development. in this study, we identified a total of 13 zhd genes (vvzhd) in the grape genome that were further classified into at least seven groups. genome synteny analysis revealed that a number of vvzhd genes were present in the corresponding syntenic blocks of arabidopsis, indicating that they arose before the divergen ...201424705465
characterization of hg-phytochelatins complexes in vines (vitis vinifera cv malbec) as defense mechanism against metal stress.an approach to understand vines (vitis vinifera) defense mechanism against heavy metal stress by isolation and determination of hg-phytochelatins (pcs) complexes was performed. pcs are important molecules involved in the control of metal concentration in plants. pcs complex toxic metals through -sh groups and stores them inside cells vacuole avoiding any toxic effect of free metals in the cytosol. the hg-pcs identification was achieved by determination of hg and s as hetero-tagged atoms. a metho ...201424715273
a novel method for evaluating flavanols in grape seeds by near infrared hyperspectral imaging.chemical composition of seeds changes during grape ripening and this affects the sensory properties of wine. in order to control the features of wines, the condition of seeds is becoming an important factor for deciding the moment of harvesting by winemakers. sensory analysis is not easy to carry out and chemical analysis needs lengthy procedures, reagents, and it is destructive and time-consuming. in the present work, near infrared hyperspectral imaging has been used to determine flavanols in s ...201424720976
genome-wide analysis and expression profile of the bzip transcription factor gene family in grapevine (vitis vinifera).basic leucine zipper (bzip) transcription factor gene family is one of the largest and most diverse families in plants. current studies have shown that the bzip proteins regulate numerous growth and developmental processes and biotic and abiotic stress responses. nonetheless, knowledge concerning the specific expression patterns and evolutionary history of plant bzip family members remains very limited.201424725365
microarray analysis of differentially expressed genes engaged in fruit development between table and wine grape.microarray analysis of genes can provide individual gene-expression profiles and new insights for elucidating biological mechanisms responsible for fruit development. to obtain an overall view on expression profiles of metabolism-related genes involved in fruit development of table and wine grapes, a microarray system comprising 15,403 ests was used to compare the expressed genes. the expression patterns from the microarray analysis were validated with quantitative real-time polymerase chain rea ...201424728608
characterization of est-derived and non-est simple sequence repeats in an f₁ hybrid population of vitis vinifera l.among different classes of molecular markers, expressed sequence tags (ests) are a new resource for developing simple sequence repeat (ssr) functional markers for genotyping and genetic mapping in f1 hybrid populations of vitis vinifera l. recently, because of the availability of an enormous amount of data for ests in the public domain, the emphasis has shifted from genomic ssrs to est-ssrs, which belong to transcribed regions of the genome and may have a role in gene expression or function. the ...201424737470
from uvr8 to flavonol synthase: uv-b-induced gene expression in sauvignon blanc grape berry.the aim of this research was to determine the effect of development and uv-b on flavonols and the regulation of gene activity in vitis vinifera l. var. sauvignon blanc grapes. particular emphasis was placed on gene activity associated with the low and high fluence uv-b responses. flavonols, particularly quercetin and kaempferol glycosides, increased substantially upon fruit exposure due to uv-b, with spatial analysis locating the changes to the berry skin. of five vvfls genes in grapes, two (vvf ...201524738597
determination of fungicides in white grape bagasse by pressurized liquid extraction and gas chromatography tandem mass spectrometry.ultrasound-assisted extraction (uae) and pressurized liquid extraction (ple) followed by gas chromatography-triple quadrupole-mass spectrometry (gc tq-ms) were used for the rapid determination of 11 fungicides (metalaxyl, cyprodinil, procymidone, iprovalicarb, myclobutanyl, kresoxim-methyl, benalaxyl, fenhexamide, tebuconazole, iprodione and dimethomorph) in white grape bagasse. the extractions were optimized on real non-spiked samples by means of experimental design and the optimal conditions w ...201424746069
micrornas in fruit trees: discovery, diversity and future research directions.since the first description of micrornas (mirnas) 20 years ago, the number of mirnas identified in different eukaryotic organisms has exploded, largely due to the recent advances in dna sequencing technologies. functional studies, mostly from model species, have revealed that mirnas are major post-transcriptional regulators of gene expression in eukaryotes. in plants, they are implicated in fundamental biological processes, from plant development and morphogenesis, to regulation of plant pathoge ...201424750383
extracellular compounds produced by fungi associated with botryosphaeria dieback induce differential defence gene expression patterns and necrosis in vitis vinifera cv. chardonnay cells.three major grapevine trunk diseases, esca, botryosphaeria dieback and eutypa dieback, pose important economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. the different fungi associated with grapevine trunk diseases can be isolated in the necrotic wood, but not in the symptomatic leaves. other factors seem to be responsible for the foliar symptoms and may represent the link between wood and foliar symptoms. one hypothesis is tha ...201424752796
genome-wide identification of wrky family genes and their response to cold stress in vitis vinifera.wrky transcription factors are one of the largest families of transcriptional regulators in plants. wrky genes are not only found to play significant roles in biotic and abiotic stress response, but also regulate growth and development. grapevine (vitis vinifera) production is largely limited by stressful climate conditions such as cold stress and the role of wrky genes in the survival of grapevine under these conditions remains unknown.201424755338
deconvoluting effects of vine and soil properties on grape berry composition.grape berry composition is influenced by several factors including grapevine and soil properties and their interactions. understanding how these factors interact to determine berry composition is integral to producing berries with desired composition. here we used extensive spatio-temporal data to identify significant vine and soil features that influence shiraz berry composition.201524756770
postharvest treatments with ethylene on vitis vinifera (cv sangiovese) grapes affect berry metabolism and wine composition.grapes (vitis vinifera, cv sangiovese), harvested at standard commercial maturity, were treated for 36 h with ethylene (et, 1000 ppm) or air (control, ct) before vinification. the composition of the grapes, must and wine was different in the ct and et samples. in the et wine, higher concentrations of specific phenol compounds, belonging to the classes of flavonols, anthocyanins, flavan-3-ols, and stilbenes, were detected. et induced a significant change in the wine aroma profile by increasing fr ...201424767053
evaluation of tannins interactions in grape (vitis vinifera l.) skins.tannins have a central role in grapevine berries both for their physiological and enological implications. in the skin tissue they can be in vacuolar solution, or associated to the cell walls through weak or strong physicochemical interactions. the present work aims to separate vacuolar, non-covalently and covalently bonded tannins fractions. a specific extraction procedure was developed. a first extraction in ethanol at low temperature allowed the quantification of vacuolar tannins. an urea tre ...201424767062
comparison of extraction protocols to determine differences in wine-extractable tannin and anthocyanin in vitis vinifera l. cv. shiraz and cabernet sauvignon grapes.cabernet sauvignon and shiraz grapes were sourced from different regions within australia, and microvinified with a skin contact period of 6 days. grape samples were extracted using two protocols: a 15% v/v ethanol, 10 g/l tartaric acid extract of gently crushed berries (wine-like, wl) and a 50% v/v ethanol, ph 2 extract of grape berry homogenate. it was found that in wl extracts, grape tannin and anthocyanin concentrations were strongly related to wine tannin, anthocyanin and color density achi ...201424773241
day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit.global climate change will noticeably affect plant vegetative and reproductive development. the recent increase in temperatures has already impacted yields and composition of berries in many grapevine-growing regions. physiological processes underlying temperature response and tolerance of the grapevine fruit have not been extensively investigated. to date, all studies investigating the molecular regulation of fleshly fruit response to abiotic stress were only conducted during the day, overlooki ...201424774299
differential proteomic analysis of grapevine leaves by itraq reveals responses to heat stress and subsequent recovery.high temperature is a major environmental factor limiting grape yield and affecting berry quality. thermotolerance includes the direct response to heat stress and the ability to recover from heat stress. to better understand the mechanism of the thermotolerance of vitis, we combined a physiological analysis with itraq-based proteomics of vitis vinifera cv cabernet sauvignon, subjected to 43°c for 6 h, and then followed by recovery at 25/18°c.201424774513
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