Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| multiple interactions of a dna-binding protein in vivo. iii. phage t4 gene-32 mutations differentially affect insertion-type recombination and membrane properties. | we have investigated in in vivo roles of t4 gene-32 protein in recombination. we have studied the effects of gene-32 mutations under conditions that allow normal dna replication and are permissive for progeny production. under these conditions, certain gene-32 mutations specifically reduce insertion-type (short-interval) recombination but none affect crossover-type (long-interval) recombination (see figure 5). heterozygote frequencies in all gene-32 mutants are similar to or higher than in a gen ... | 1977 | 885343 |
| [the treatment of carcinoma of the tongue (author's transl)]. | in the past 18 years 34 patients (21 males and 13 females) with carcinoma of the tongue were treated in the author's clinic. patients with t1n0 lesions were treated satisfactorily only with irradiation. patients with advanced disease, such as t2-t4 with metastases, were classified into 2 groups. one group was treated surgically (hemiglosectomy and radical neck dissection) and postoperative irradiation (about 6000 rads). the other group was treated with preoperative irradiation (about 4000 rads) ... | 1977 | 885744 |
| depressed thyroid function in two tetraplegic dogs. | rapidly progressing tetraplegia developed in two 6-year-old dogs, both of which had eaten decayed meat sometime during the 24 hours prior to the onset of paralysis. both dogs were found to have depressed thyroid function, as indicated by extremely low triiodothyronine (t3) and thyroxine (t4) values. electromyographic studies revealed some abnormal insertional activity but no abnormal potentials when the fore- and hindlimb muscles were at total rest. nerve conduction velocity was slightly depress ... | 1977 | 885770 |
| [the results of cobalt therapy for carcinoma of the larynx (author's transl)]. | the effectiveness of cobalt therapy for carcinoma of the larynx in demonstrated by the results obtained: 53% overall survival at 5 years, with 66% for carcinomas of the glottis, and 48% for supra-glottal lésions. the dose-time ratio used was adapted to the tumor category and to the individual reactivity of the patients, local control being obtained in 100, 87, 65 and 25% for t1, t2, t3 and t4 tumours respectively. | 1977 | 886530 |
| lambda and t4 bacteriophage hybrids. | 1977 | 886622 | |
| [results of surgical treatment of cancer of the large intestine]. | the results of surgical treatment of 765 patients with colonic cancer are analysed, the resectability was 68.7%, postoperative mortality--in 16.3%. the most frequent causes of lethal issues were peritonitis and acute cardiovascular disturbances. the survival of 5 years and longer after radical operations was noted in 53.4 +/- 3.4%. the survival rate in patients with cancer of the right colon is reliably higher than that with the left colon involved. hemicolectomy yielded better results compared ... | 1977 | 888418 |
| thyroid function tests in the elderly in the community. | a study of the well elderly living at home has demonstrated that the ranges for serum thyroxine (t4), triiodothyronine (t3) uptake and free-thyroxine index (fti) are much narrower than those for the sick elderly in-patient. the prevalence of thyroid disease appears similar in both types of elderly population. | 1977 | 888746 |
| the families of patients with acute rheumatic fever or glomerulonephritis in trinidad. | the families of 21 patients with acute rheumatic fever (arf) and 44 patients with acute glomerulonephritis (agn) in trinidad were examined in their homes. the arf and agn families were equally large and crowded and they lived in the same largely rural areas. however, only 22% of the arf family members had skin infections in contrast to 61% of the agn family members. sixty-eight per cent of skin infections in arf families and 69% of skin infections in agn families yielded group a streptococci. th ... | 1977 | 888815 |
| multiple interactions of a dna-binding protein in vivo. i. gene 32 mutations of phage t4 inactivate different steps in dna replication and recombination. | 1977 | 889598 | |
| [isolation and properties of micrococcus luteus endonucleas acting in dna depurinization but inactive with pyrimidine dimers]. | an endonuclease (ap-endonuclease ii) that specifically attacks double stranded or single stranded depurinated dna, resulting in single-strand nicks, has been purified 320-fold from micrococcus luteus. the enzyme is not stimulated by 0.002 m mgcl2, it induces 3'oh-5'po4 breaks on the 5' side of apurinic sites, it has no activity towards uv-irradiated dna and has a molecular weight of about 30 000. in cooperation with dna-polymerase from m. luteus and t4 dna ligase, ap-endonuclease ii has been sho ... | 1977 | 889977 |
| the toxic effect of small iodine supplements in patients with autonomous thyroid nodules. | in sixteen cases of toxic adenoma of the thyroid (autonomous hot nodule with complete suppression of the surrounding normal parenchyma) potassium iodide was given in doses of 100 microgram/day for one week, 200 microgram/day for another and 400 microgram/day for a third week. there was a progressive increase in the serum t4 level. serum t3 also increased, although this was significant only after the first week. serum tsh was undetectable throughout the entire period of the study. this metabolic ... | 1977 | 890996 |
| bacteriophage t4 mutants which propagate on e. coli k12 but not on e. coli b. | 1977 | 891859 | |
| mutator mutations in bacteriophage t4 gene 42 (dhmc hydroxymethylase). | temperature-sensitive mutations of bacteriophage t4 gene 42 produce diverse effects upon spontaneous mutation rate. g:c vector a:t transition rates are increased, often strongly; frameshift mutation rates are weakly increased; a:t vector g:c transition rates (and perhaps also a:t vector py:pu transversion rates) are decreased; and one g:c vector py:pu transversion rate is also decreased. these results, together with certain interactions between gene-42 mutator effects and both base analogue muta ... | 1977 | 892421 |
| the evolution of epistasis and the advantage of recombination in populations of bacteriophage t4. | experiments reported here test two hypotheses about the evolution of recombination: first, the fisher-muller concept that sexual organisms respond to selection more rapidly than do asexual ones, and second, that epistasis is more likely to evolve in the absence of recombination. populations of bacterio-phage t4 were selected by the drug proflavine in discrete generations and the change in mean population fitness was monitored. three separate selection series yielded results supporting the fisher ... | 1977 | 892424 |
| [recombination in amber mutants of bacteriophage t4b. iii. influence of x-mutation on intragenic recombination in t4 phages]. | study was made of the influence of x-mutation on interallelic and intergenic recombination at the region of genes 34-38 of phage t4b. in the case of intergenic crosses in all studied recombinations of genes mutation x caused a 2-fold decrease in recombination frequency. x-mutation showed the same effect on integenic recombination in genes 36 and 38. in the case of crossing flank markers of gene 35 the frequency of recombination decreased 3.5-fold, that of gene 34--6 fold. crosses of double am-x ... | 1977 | 892435 |
| affinity labeling of human serum prealbumin with n-bromoacetyl-l-thyroxine. | affinity labeling of human serum prealbumin with n-bromoacetyl-l-thyroxine (bract4) was used to investigate the binding domain for l-thyroxine (t4) on prealbumin. fluorescence titration with 8-anilinonaphthalene-1-sulfonate revealed a strong and a weak binding site for bract4 (k1 = 1 x 10(8) m-1; k2 = 1 x 10(6) m-1). the reaction of bract4 with prealbumin to form a covalent bond was inhibited in the presence of t4 and binding of t4 to prealbumin was nearly abolished after affinity labeling with ... | 1977 | 893396 |
| thyrotoxicosis detected in a mass-screening program for neonatal hypothyroidism: demonstration of placental transfer of an immunoglobulin with marked lipolytic activity. | a female infant born to a mother with active graves disease and a strongly positive lats developed neonatal thyrotoxicosis. the diagnosis was confirmed rapidly by the markedly elevated filter paper spot t4 (5.45 ng/40 microliter) taken on the fifth day of life as part of the quebec screening program for neonatal hypothyroidism. although the infant's serum was negative for lats, it reacted strongly (as did the maternal serum) in the lipolytic assay for lats. this case illustrates the ability of s ... | 1977 | 894408 |
| bacteriophage t4 virion baseplate thymidylate synthetase and dihydrofolate reductase. | additional evidence is presented that both the phage t4d-induced thymidylate synthetase (gp td) and the t4d-induced dihydrofolate reductase (gp frd) are baseplate structural components. with regard to phage td it has been found that: (i) low levels of thymidylate synthetase activity were present in highly purified preparations of t4d ghost particles produced after infection with td(+), whereas particles produced after infection with td(-) had no measurable enzymatic activity; (ii) a mutation of ... | 1977 | 894793 |
| rabbit liver trna1val:i. primary structure and unusual codon recognition. | the major valine acceptor trna1val from rabbit liver was purified and its nucleotide sequence determined by in vitro [32p] - labeling with t4 phage induced polynucleotide kinase and finger-printing techniques. its primary structure was found to be identical with the major valine trna from mouse myeloma cells. according to the wobble hypothesis this trna, which exclusively has an iac anticodon, should decode the valine codons guu, guc and gua only. however, this trna recognizes all four valine co ... | 1977 | 896481 |
| comparative oligonucleotide fingerprints of three plant viroids. | 5' phosphorylation in vitro with gamma-32p-atp and t4 phage induced polynucleotide kinase was used to obtain rnaase a and rnaase t1 fingerprints of three plant viroids: potato spindle tuber viroid from tomato (pstv-tom), chrysanthemum stunt viroid from cineraria (chsv-cin) and citrus exocortis viroid from gynura aurantiaca (cev-gyn). these three viroids differ significantly from each other as judged from their oligonucleotide patterns. this supports the concept of individual viroid species. | 1977 | 896482 |
| [production of antibodies for t3 and t4 (author's transl)]. | 1977 | 897426 | |
| [results of radical radiotherapy in breast cancer]. | in 17790 examinations over a 5-year period, 1174 breast carcinomas have been detected and treated in the department of senology, university hospital, strasbourg, france. 467 carcinomas were treated by radiotherapy alone. five-year survival has been studied. over half of the carcinomas treated were t3 and t4-sized. overall survival is 46%. | 1977 | 897631 |
| [contribution of radiotherapy in the local-regional treatment of breast cancer]. | the role of radiotherapy in the treatment of mammary carcinoma is reviewed. the indication for postoperative radiotherapy in cases of primary tumors t2 and t3, particularly in the presence of metastatic lymph nodes (n1), is still given. for stage 1 (t1/t2 n0), postoperative radiotherapy may be omitted or can be limited to the lymphatic area which has not been surgically explored. pre-operative radiotherapy for the more advanced stage (t3/t4 and n2) has proven more effective than postoperative ir ... | 1977 | 897644 |
| [biological aspects of endemic goiter in the pyrennes atlantiques (author's transl)]. | in an endemical goiterous area (oloron sainte-marie 64, france) a thyroid investigation has been realised on a certain number of people having a goiter (g) and those without goiter (n.g.). various explorations such as t4 d, r t3 u, tsh ria, t3 ria. achilles' reflex, cholesterol have shown no significant difference nor any abnormality inspite of a global deficit in iodine (35 ng/24 h). it can only be retained an absence in negative corelation t3 tsh (p less than 0.05). | 1977 | 900873 |
| the two triiodothyronines (t3 and rt3). thyroid biosynthesis of t3 and rt3 and peripheral metabolism of thyroxine (author's transl)]. | thirty per cent of the iodine in thyroglobulin is present as iodothyronines. l-thyroxine (t4) represents 90-95% of hormonal iodine, 3,5,3'-triodo-l-thyronine (t3) contains at the most two per cent of it, 3,3'5'-triodo-l-thyronine (rt3) even less, as well as traces of 3,3'-diodo-l-thyronine. the plasma concentration of t4 is about 8 microgram per 100 ml, in the case of t3 it is 120 ng and 25 ng for rt3. the cell nucleus preferentially binds t3 and rt3 and there are also some specific mitochondria ... | 1977 | 900879 |
| synthesis and biological activity of a lambda pseudo operator. | the chemical and enzymatic syntheses of bacteriophage lambda pseudo operator dna are described. the 17 base-paired duplex contains the dna which has been proposed as the binding site for ci repressor protein. the synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six proposed operator sites in the leftward and rightward operators. however, it does not correspond exactly to any single proposed operator sequence. the chemical synthesis includes the d ... | 1977 | 901770 |
| effects of thyroidectomy and thyroxine on plasma growth hormone and insulin levels in rats. | plasma growth hormone and insulin levels were measured in normal, thyroidectomized (tx), and tx rats treated with 1-thyroxine (1-t4) or rat-growth hormone (r-gh). although normal growth was observed 5 days after surgery, a decrease of circulating levels of gh was evidenced as early as 5 days after the operation. however, the plasma insulin levels were not affected by the 5th, 10th and 15th day of thyroidectomy. with a more prolonged thyroid hormone deprivation, the plasma insulin levels of tx ra ... | 1977 | 903076 |
| localization of minor protein components of the head of bacteriophage t4. | the bacteriophage t4 capsid contains a number of minor proteins that are required for head assembly but whose detailed function and position in the head are unknown. we have found that by systematically varying the conditions of extraction, some of these minor proteins can be removed while the main capsid structure is left substantially intact. electron microscopic examination of the residual capsids showed that the extraction of the product of gene 20 is correlated with the loss of a plug that ... | 1977 | 904022 |
| properties of the nonlethal recombinational repair x and y mutants of bacteriophage t4. ii. dna synthesis. | the bacteriophage t4 recombination-deficient mutants x and y exhibited decreased rates of dna synthesis as compared to wild-type t4. mutant-induced dna synthesis was more sensitive to mitomycin c than was wild-type synthesis. however, dna synthesis in mutant- and wild-type-infected cells exhibited the same sensitivity to uv light and x-irradiation. when high-specific-activity label was administered at various times postinfection, mutant dna synthesis resembled that of wild type for 12 min. after ... | 1977 | 904025 |
| long-term observation of thyroid function after surgical treatment of thyrotoxicosis. | during the 10-year period from 1960 to 1970 a total of 213 patients with diffuse or nodular toxic goitre underwent subtotal thyroidectomy. a short-term preoperative treatment with antithyroid drugs and iodine was given systematically. the observation period ranged from 5 to 15 years, average 9 years. a total of 203 patients (95%) were traced and followed up. the concentration of se-t4, se-t3, and se-tsh was measured and in selected cases a trh test and bmr was carried out in addition. all thyroi ... | 1977 | 906756 |
| induction of the thyroxine (t4) to triiodothyronine (t3) converting enzyme in rat liver by thyroid hormones and analogs. | in thyroidectomized, unsubstituted rats the t4 to t3 converting activity of liver homogenate is reduced to about 30% of that in unoperated control animals. the enzyme activity can be reinduced dose-dependently with t4. to achieve a normal activity, high, non-physiological plasma t4 concentrations are needed. plasma t3 levels are much better correlated to the t4 to t3 converting activity. pure t3 proved to be a more potent enzyme inducer than t4. no difference could be detected between l- and d-t ... | 1977 | 908148 |
| the behaviour of thyroid hormones in an infant with untreated neonatal thyrotoxicosis. | the clinical course from birth and serial measurements of serum t3, t4 and tsh in an infant with untreated neonatal thyrotoxicosis are reported. the thyroid hormone levels fell exponentially with time at rates very much slower than those previously reported for the maternally-transmitted thyroid stimulating antibody generally thought to cause the disorder. steady physiological levels of thyroid hormones were achieved after 110 days (serum t3 = 3.4 nmol/l, t4 = 118 nmol/l). tsh first rose to a me ... | 1977 | 908154 |
| a simple synthesis of [3,5-125i]diiodo-l-thyroxine of high specific activity. | the customary methods for labeling t4 and its analogs, iodination or exchange-labeling, are not applicable to those iodothyronines whose iodine atoms are exclusively in the nonphenolic ring. therefore, [3,5-125i]diiodo-l-thyronine had to be synthesized by a different metnod. this synthesis involves the coupling of [125i]diiodo-l-tyrosine with 4-hydroxy-3,5-diiodophenylpyruvic acid to form l-thyroxine labeled in the nonphenolic ring, followed by removal of the two unlabeled iodine atoms in the ph ... | 1977 | 908276 |
| extra-long bacteriophage t4 tails produced under in vitro conditions. | 1977 | 909089 | |
| triiodothyronine and thyroxine content of desiccated thyroid tablets. | triiodothyronine (t3) and thyroxine (t4) were measured by radioimmunoassay in pronase hydrolysates of four lots each of 1- and 2-grain tablets of desiccated thyroid (thyroid, armour) and thyroglobulin (proloid, warner-chilcott). the methodology used was verified by studies of tablets containing known quantities of t4 and t3. one grain of desiccated thyroid contained 12 +/- 1 and 64 +/- 3 microgram (mean +/- sd) of t3 and t4 per tablet, respectively (t4/t3 molar ratio, 4.3). a 1-grain tablet of t ... | 1977 | 909397 |
| the nucleotide sequence of two restriction fragments located in the gene ab region of bacteriophage s13. | the nucleotide sequence of a double stranded dna fragment from the gene ab region of bacteriophage s13 dna has been determined. the fragment was isolated as two adjacent shorter fragments by cleavage of s13 replicative form (rf) dna with restriction endonuclease iii from hemophilus aegyptius. the strands of the fragments were separated electrophoretically and hydrolyzed with t4 endonuclease iv to yield short oligonucleotides which were then sequenced by partial exonuclease digestion. the complet ... | 1977 | 909772 |
| nucleotide sequence of 3' untranslated portion of human alpha globin mrna. | we have determined the nucleotide sequence of 75 nucleotides of the 3'-untranslated portion of normal human alpha globin mrna which corresponds to the elongated amino acid sequence of the chain termination mutant hb constant spring. this was accomplished by sequence analysis of cdna fragments obtained by restriction endonuclease or t4 endonuclease iv cleavage of human globin cdna synthesized from globin mrna by use of viral reverse transcriptase. analysis of crna synthesized from cdna by use of ... | 1977 | 909779 |
| stability of phage t4 lysozymes. i. native properties and thermal stability of wild type and two mutant lysozymes. | two mutants of phage t4 lysozyme were prepared and characterized. one mutation substituted a tyrosine residue for tryptophan at position 138. the other substituted tyrosines at all three tryptophan positions of the wild type molecule (126, 138, 158). comparative studies of the physical properties (absorption, fluorescence, circular dichroism) of the three enzymes were performed as a function of ph. also, the proteins were reversibly melted as a function of ph. since the unfolding reaction appear ... | 1977 | 911878 |
| molecular arrangement of dna in bacteriophage t4. | 1977 | 912002 | |
| [double antibody radioimmunoassay of t4 and t3 using "eiken" kit]. | 1977 | 912936 | |
| ["hypothyroid" goitre in puberty treated with a combined t3/t4 preparation (author's transl)]. | after administration of combined t3/t4 preparations (ratio of 4 : 1) to juveniles with goitre, size ii-iii (who), and hypothyroid t4 or pbi levels, the t3 value reached hyperthyroid levels 7-8 hours afterwards. the increased t3 level with normal t4 blood levels can lead to iatrogenic t3 hyperthyroidism. serial tests demonstrated that at increasing doses a raised t3/t4 ratio, which is observed with low t4 values, can return to normal but with increasing doses this ratio, caused by t3 rise, can ag ... | 1977 | 913268 |
| [quantitative aspects of the formation of a half chromatid chiasma as an intermediate product of genetic recombination in bacteriophages]. | formal genetic consequences of generation and break-up of a half chromatid chiasma have been studied on the basis of a model with the symmetric exchange of two strands between parental dna molecules. in recombination analysis, generation and break-up of this structure gives rise to a critical frequency r (zeta), which corresponds to recombination between two markers separated by a distance equal to the mean genetic length zeta of the hybrid region at the time of scission of the cross connection. ... | 1977 | 914034 |
| u-g-a suppressor of bacteriophage t4 associated with arginine transfer rna. | a u-g-a suppressor of bacteriophage t4, designated psu4+op, has been isolated and characterized. the transfer rna species previously shown to have an anticodon sequence complementary to arginine codons is affected by the psu4+op mutation. wild type and psu4+op arginine trnas have the same sequence except for their anticodons, where u-c-u in the wild type species is mutated to u-c-a in the psu4+op species. this mutation is believed to confer u-g-a suppressor activity on the psu4+op arginine trna. | 1977 | 914869 |
| ultracentrifugal characterization of cytosol-binding proteins for thyroxine and triiodothyronine in human liver. | sucrose density gradient ultracentrifugation was performed on human liver cytosol containing double tracers of 131i-thyroxine(t4) and 125i-triiodothyronine(t3) together with or without large amount of stable hormones. tracer t4 bound to the cytosol indicated 2 peaks of 8.3s and 4.7s, both of which were displaced with stable t4, but not with t3. maximal binding capacity of the 8.3s component averaged 43.2 microgram/100ml of 33% cytosol. tracer t3 in the cytosol showed a single peak of 4.2s which ... | 1977 | 914987 |
| particle counting immunoassay (pacia). i. a general method for the determination of antibodies, antigens, and haptens. | by using a device designed for counting blood cells, it is possible to measure the agglutination of polystyrene beads (0.8 mu) with accuracy and great sensitivity, the agglutination resulting in a reduction in the number of particles. the latter coated with antigen can be used for determining igm or igg antibodies e.g. human rheumatoid factor or rabbit anti-bovine serum albumin antibodies. macromolecules with multiple antigenic determinants agglutinate particles carrying specific antibodies. thi ... | 1977 | 915310 |
| distinctive protein requirements of replication-dependent and -uncoupled bacteriophage t4 late gene expression. | this paper further explores the relationship of viral dna replication to bacteriophage t4 late gene expression. it is shown that replication coupled and -independent late transcription make different qualitative or quantitative demands on phage protein synthesis. in further analysis of these different protein synthesis requirements, experiments were performed with a temperature-sensitive mutant in t4 gene 55 (ts553). it is known that the gene 55 product regulates t4 late gene expression and bind ... | 1977 | 916023 |
| isolation and mapping of t gene mutants of bacteriophage t4d. | a procedure for selective isolation of t4 t mutants is described. at 120 min after infection of escherichia coli cells with a low multiplicity of t4 bacteriophage, the mixture was sedimented through a linear sucrose gradient, and infected cells that remained intact were collected as the fastest sedimenting fraction. ten to 50% of the phage released by chloroform treatment of this fraction were t mutants. collection of a high proportion of t mutants depended on efficient elimination of cells that ... | 1977 | 916026 |
| thyroid function in children with chronic renal failure. | thyroid function was evaluated in 24 children (aged 4-18 years) with chronic renal failure either before institution of hemodialysis or after more than 3 months of hemodialysis. 22 patients were clinically euthyroid and 2 were hypothyroid; in one case hypothyroidism was secondary to cystinosis and in the other it followed radiation therapy. the 2 hypothyroid patients had subnormal levels of t4, t3, fti and ft4 as well as elevated serum tsh levels. mean values for t4, t3, fti and ft4 for the rema ... | 1977 | 917171 |
| [screening for hypothyroidism in the newborn with a total t 4-ria method (author's transl)]. | a screening method for detection of congenital hypothyroidism is presented in detail in cooperation with the "austrian program for inborn errors of metabolism". screening is performed by measuring the total t4-content of 1/8 inch filter paper dots filled with dried blood of newborns. the strategy for recall of newborns with borderline or pathological t4-values used, results in a definite diagnosis on day 55 of life. the advantages of additional tsh determination in the filter paper dots are disc ... | 1977 | 917574 |
| studies on replacement and suppressive dosages of 1-thyroxine. | serum thyrotropin (tsh) levels were studied in 55 hypothyroid patients in order to determine adequate replacement and suppression dosages of 1-thyroxine (t4). in accordance with previous reports it was found that most patients had normal tsh levels and were clinically euthyroid at daily doses of 0.10-0.15 mg t4. none of the patients required a dose exceeding 0.20 mg. when the tsh levels normalized, serum thyroxine and serum triiodothyronine also fell to levels within their normal ranges. the eff ... | 1977 | 920242 |
| [serum thyroxine determination: comparison and clinical significance of radioimmunoassay and competitive binding assay (t4-ria pack and thyopac-4)]. | 1977 | 923114 | |
| effects of diuretics on thyroid function of guinea pigs. | uptake of radioiodide by the isolated thyroid and serum concentrations of thyroxine (t4) and tri-iodothyronine (t3) were determined in guinea pigs pretreated with ethacrynic acid (20 mg/kg), furosemide (40 mg/kg) or hydrochlorothiazide (40 mg/kg). it was found that both ethacrynic acid and furosemide suppressed the 131i uptake by the isolated thyroid tissues. in addition, thyroid weight and serum t3 concentration were lower in ethacrynic acid-treated animals. it seems that some diuretics, partic ... | 1977 | 923646 |
| triiodothyronine-producing metastatic follicular thyroid carcinoma. | thyroid hormone serum concentration was determined in a thyroidectomized patient with functional follicular thyroid carcinoma with metastases. marked diminution of circulating serum t4 and ft4 with normal t3 serum concentration was found. the clinical implications of this shift in secretory activity are discussed. | 1977 | 924359 |
| priming of deoxyribonucleic acid synthesis on phage fd and phix174 templates by oligoribonucleotides contaminating nucleoside 5'-triphosphates. | commercial preparations of guanosine 5'-triphosphate and deoxyguanosine 5'-triphosphate are contaminated with oligoribonucleotides 4 to 6 residues in length. the oligoribonucleotides can be separated from the nucleoside 5'-triphosphates by chromatography on deae-sephadex a-25 or by gel filtration through sephadex g-25. the oligoribonucleotides are effective primers for the dna polymerase of bacteriophage t7 on the single-stranded circular dnas of phage fd and phix174; they are covalently attache ... | 1977 | 925008 |
| role of deoxycytidylate deaminase in deoxyribonucleotide synthesis in bacteriophage t4 dna replication. | 1977 | 925015 | |
| serum thyroxine concentrations in larval and metamorphosing anadromous sea lamprey, petromyzon marinus l. | thyroxine (t4) concentrations in samples of fresh sera taken from fed and unfed larval (ammocoetes) and metamorphosing anadromous sea lamprey (petromyzon marinus) were measured by means of radioimmunoassay. t4 levels in unfed ammocoetes after a 1-month period were not significantly different from those of the fed ammocoetes. nutritional state of ammocoetes therefore does not seem to affect their serum t4 levels. serum t4 concentrations decreased abruptly at the onset of metamorphosis and decline ... | 1977 | 925662 |
| upper alveolar carcinoma--a 30 year survey. | primary malignancy of the upper alveolus is an infrequent type of intra-oral carcinoma. this study reviews in detail 50 patients with a primary tumor of the upper alveolus who were treated at the cancer control agency of british columbia and vancouver area hospitals, between 1940-1970 inclusive. sixteen per cent had exposure to possible industrial carcinogens; 34 per cent had previous leukoplakia. the common presenting symptoms were pain (78 per cent) and swelling (64 per cent). only 22 per cent ... | 1977 | 926221 |
| genetic identification of cloned fragments of bacteriophage t4 dna and complementation by some clones containing early t4 genes. | bacteriophage t4 dna containing cytosine has been obtained from cells infected with phage mutant in genes 42, 56, dena and denb. this dna can be cut by a number of restriction endonucleases. fragments obtained by digestion of this dna with ecori have been cloned using the vector plasmid pcr1. clones containing t4 dna were identified by hybridization with radioactive early and late t4 rna. a simple marker rescue technique is described for the genetic identification of the cloned t4 fragments. som ... | 1977 | 927440 |
| [thyrotropic activity of the pituitary gland in rats at various age periods]. | the thyrotropic activity of the hypophysis (tth synthesis and secretion) was studied in rats at various age periods. the work was conducted on mongrel male rats of four age groups: 1 1/2-2, 8-10, 18-24, and 28-32-months old. being relatively low in the sexually immature animals, the thyrotropic activity of the hypophysis considerably intensified in adult rats and diminished with the ageing of the organism. a reduction of the tth synthesis and secretion in old animals, testified to by the hormone ... | 1977 | 928330 |
| [standardization of a new thyroid gland suppression test by means of 3000 microgram l-thyroxine]. | the suppressibility of the tsh-thyroid axis one week after a single dose of 3000 microgram l-thyroxine was studied in 21 euthyroid subjects. in all cases the normal thyroid function was proven by euthyroid values for thyroxine (t4), free thyroxine index (ft4-i), triiodothyronine (t3), radioiodine uptake, pbi-131, a normal scintiscan and by a physiologic tsh response to thyrotropin-releasing hormone (trh). compared to the baseline values the mean suppression of radioiodine uptake at 2,24 and 48 h ... | 1977 | 929149 |
| [primary coblat therapy and secondary surgery for supra-glottal carcinoma (author's transl)]. | this statistical study concerned 218 carcinomas of the laryngeal vestibule and margin treated at the curie foundation between 1958 and 1967, by primary cobalt therapy followed when necessary by secondary total laryngectomy. it shows that cobalt therapy alone gives excellent results in early forms (t1 and t2) even in the presence of n1 or n2 nodes. by contrast, the proportion of cures is much lower in t3 and t4 forms. secondary total laryngectomy was used in only 25 cases out of 127 failures. the ... | 1977 | 931295 |
| an assessment of daily production and significance of thyroidal secretion of 3, 3', 5'-triiodothyronine (reverse t3) in man. | while 3, 3', 5'-triiodothyronine (reverse t3, rt3) has been detected both in human serum and in thyroglobulin, no quantitative assessment of its metabolic clearance rate (mcr), production rate (pr), or secretion by the thyroid is yet available. this study examines this information in euthyroid subjects and evaluates it in light of similar information about two other iodothyronines in the thyroid: 3, 5, 3'-triiodothyronine (t3) and thyroxine (t4). thus, it was noted that rt3 is cleared from human ... | 1976 | 932209 |
| [citrate values in the whole blood of patients with malignant tumors of various locations]. | based on reports on elevated concentrations of 2-oxoglutarate in the blood of human subjects with tumors of various locations, data are presented on the concentration of citrate and pyruvate in the whole blood of 41 patients with malignant tumors (mainly of the female breast and of the digestive tract), 30 patients with benign surgical diseases and 12 healthy subjects. adopting a level of significance of 2p=0.10 differences of concentration were found between the following collectives: (1) the m ... | 1976 | 933983 |
| [value of carcino-embryonic antigen assay in the evaluation and surveillance of breast cancer]. | with the method used, the concentrations of carcinoembryonic in antigen serum can be said to be abnormal above 10 ng/ml. the concentrations are normal in benign mastopathies. in 131 cases of breast cancer were studied, they are high in 8 p. cent of t1 and t2 breast cancers, and in 53.9 p. cent of t3 and t4 cancers. they are high in 22 p. cent of cases in the first perceptible phase of their disease, and 92 p. cent relapsed cases. they are also high in 23 p. cent of cases without metastasis, in 3 ... | 1976 | 934839 |
| thyroxine and triiodothyronine in blood after ingestion of iodinated casein by chicks. | white leghorn cockerels were fed a broiler ration containing 0 (controls), 0.02 or 0.04% protamone (iodinated casein) either continuously or for 10-14 days after which the 0.02% protamone ration was switched to control or vice versa. body growth rate was temporarily suppressed during the first 14 days of feeding 0.02% protamone. thyroid weight was promptly depressed during feeding of protamone and increased more slowly when protamone was removed from the feed. only a fleeting increase in oxygen ... | 1976 | 935013 |
| [irradiation of the thyroid gland: changes in its function]. | the authors have studied some usual parameters of the evaluation of the thyroid gland function, after secondary irradiation during tele-cobaltherapy treatment in 20 patients with larynx carcinoma. the parameters studied have been iodine uptake, hamolsky index, t4, ft4 pb127i and cholesterol. the values, statistically treated, enable the conclusion that normal adult thyroid gland has a very low clinical and analytical radiosensibility. | 1976 | 935726 |
| statistical manipulation for normalization of data: in vitro thyroid function tests. | interest in chemical and statistical methods chosen to define "normal" populations for the clinical significance of tests has grown recently. because few clinical values are distributed in gaussian fashion, recommendations for smoothing of data by transforms have been prepared. in this paper we examine mathematical transformations as they are applied to in vitro tests of thyroid function and evaluate the use of multivariate regression analysis. mathematical transforms used included square root, ... | 1976 | 935913 |
| genetic analysis of t4 tail fiber assembly. i. a gene 37 mutation that allows bypass of gene 38 function. | 1976 | 936481 | |
| effect of ts mutation in gene 43 of bacteriophage t4 on recombination of the bacteriophage. | certain ts mutations in gene 43 of bacteriophage t4 are known to exhibit mutator or antimutator activities with respect to different rii mutations of this phage. the effect of these mutations on recombination frequencies of double mutant strains of phage t4 with genotype rii ts--homoallelic with respect to the ts trait--was examined. the results implicate the essential role of the genetic background in the investigated process. | 1976 | 937086 |
| purification and properties of bacteriophage t4-induced rna ligase. | 1976 | 938049 | |
| thyroid function and insulin secretion from the perfused pancreas in the rat. | the influence of thyroid function on the kinetics of glucose-induced insulin secretion from the isolated perfused rat pancreas has been studied. l-thyroxine (l-t4) administration did not modify the immediate insulin secretory response of the perfused pancreas to glucose. l-triiodothyronine (l-t3) treatment as well as propylthiouracil (ptu) treatment decreased the immediate insulin secretory response of the pancreas slightly. only thyroidectomy (tx) reduced the immediate secretory response of the ... | 1976 | 939192 |
| variations in the response of the thyroid gland of the rat to different low-iodine diets: correlation with iodine content of diet. | large variations are frequently encountered in the thyroidal responses of rats to commercially available low-iodine diets. the major aim of this investigation was to attempt to correlate these variations with differences in the iodine content of the diets. a method was developed for measuring the iodine content of low-iodine diets which was sufficiently sensitive to discriminate between a diet containing 15-20 ng of iodine per g and one containing 30-40 ng of iodine per g. large differences were ... | 1976 | 939196 |
| formation of 3,3'-diiodothyronine and 3',5',3-triiodothyronine (reverse t3) in thyroid glands of rats and in enzymatically iodinated thyroglobulin. | it was observed in the present investigation that labeled thyroxine (t4) comprised less than 2% of the total 131i in the thyroids of severely iodine-deficient rats labeled with 131i for 18-24 h, a much lower value than had previously been reported for iodine-deficient rats. this low value was attributable to two factors: 1) the use of a diet low enough in iodine content to produce extreme iodine deficiency, and 2) the use of a paper chromatography system that successfully separates t4 from the m ... | 1976 | 939197 |
| decreased pituitary growth hormone content in rats treated neonatally with high doses of l-thyroxine. | growth hormone and thyrotrophic hormone content have been measured by specific radioimmunoassays in anterior pituitaries of 22 day old rats. these animals were injected with saline or very high doses of l-thyroxine during the neonatal period in order to induce the "neo-t4" syndrome. growth of such animals is known to be affected. it was found that not only tsh but also gh content of the t4-treated animals was significantly lower than that of the saline-injected controls. | 1976 | 939463 |
| serum thyrotropin, prolactin, and growth hormone levels during the early neonatal period in the human infant. | normal term newborn infants have been studied during the first four hours after birth. cord blood and brachial venous blood samples were collected for measurement of serum prolactin (n = 15; 11 vaginal delivery; four cesarean section delivery), thyrotropin (n=8), growth hormone (n=7), tri-iodothyronine (n=7) and thyroxine (n=7). the mean cord serum tsh concentration was 12 +/- 2 muu/ml and peaked in all infants 30 minutes after birth (67 +/- 7 muu/ml). blood prl also increased in all of the 15 i ... | 1976 | 940027 |
| a mutation that confers temperature sensitivity on the translation of riib in bacteriophage t4. | 1976 | 940158 | |
| separation of labelled iodide, iodoprotein, iodotyrosines, and iodothronines in serum from patients treated with iodine-131, using an improved chromatographic method. | a method is described for complete separation of iodoprotein, 3-iodotyrosine (mit), 3,5-diiodotyrosine (dit), 3,5,3-triiodo-l-thyronine (t3), and l-throxine (t4) on a single column run on sephadex g-25 superfine in alkaline solution. sera from patients treated with 131i- have been analysed by this method after removal of i- by resin dialysis. | 1976 | 940981 |
| site of cleavage of superhelical phix174 replicative form dna by the single strand-specific neurospora crassa endonuclease. | experiments with the neurospora crassa single strand-specific endonuclease have provided evidence for the existence of regions of partially single-stranded character in covalently closed superhelical replicative form dna of phix174. the nuclease converts the superhelical molecules to either singly hit relaxed circular or doubly hit linear molecules. we show that the initial cleavage of phix174 superhelical dna is a "nick" bounded by a 5'-phosphate and a 3'-hydroxyl; no nucleotides are excised as ... | 1976 | 942717 |
| triiodothyronine radioimmunoassay and its application to thyroid disorders. | an accurate, highly sensitive triiodothyrone (t3) radioimmunoassay system is described. direct measurement in serum is made possible by the use of t3-free serum for standards, and blocking of t3 binding to thyroxine (t4)-binding globulin with salicylate. t4 cross reactivity was less than 0.3%. mean t3 levels in 55 euthyroid, 32 hyperthyroid and 19 primary hypothyroid patients were 1.46 +/- 0.17, 5.34 +/- 1.86 and 0.49 +/- 0.34 (sd) ng/ml, respectively. t3 was found to be a more sensitive index t ... | 1976 | 943381 |
| biochemistry of dna-defective mutants of bacteriophage t4. vi. biological functions of gene 42. | bacteriophage t4 gene 1 and 42 amber mutants (defective in deoxynucleoside monophosphate kinase and deoxycytidylate hydroxymethylase, respectively) are able to synthesize dna in cell-free lysates prepared as described by barry and alberts (1972), in contrast to their inabliity to do so in plasmolyzed and toluenized cell systems. addition of extracts containing an active gene 1 or 42 product has no effect on synthesis in lysates defective in the respective gene. thus, if these enzymes do play add ... | 1976 | 943575 |
| transcriptional control of t4 coliphage-specific genes 30, 42, 43, riia, riib, and e. | escherichia coli b/r (suo) was infected, at 30 degrees c, with t4dam+, t4damb24-amn82 (i-, 44-, dna-negative phenotype), and t4damn134ambl292 (33-, 55-, maturation-defective phenotype). a genetic ('transformation') assay was used to monitor transcription of genes 30 (polynucleotide ligase), 42 (deoxycytidylate hydroxymethylase), 43 (dna polymerase), riia, riib, and e (endolysin). the principal results are: (i) all of the genes studied were transcribed exlusively from the so-called l-strand of ph ... | 1976 | 945327 |
| ehanced biliary thyroxine excretion in rats treated with pregnenolone-16alpha-carbonitrile. | normal rats were treated with pregnenolone- 16alpha - carbonitrile (pcn) 10 mg/100 g by stomach tube twice daily for 3 days. in these animals the biliary excretion of intravenously injected 125i-thyroxine (t4) was enhanced and the bile: plasma 125i ratio (b/p ratio) and the biliary clearance rate of plasma 125i-t4 was increased. normal rats were treated with pcn for 3 days and homozygous gunn rats for 13 days. in both groups pcn enhanced the bile flow and elevated the b/p ratios and the biliary ... | 1976 | 946136 |
| iodothyronine content of human thyroid albumin. | stable thyroid hormones (t4 and t3)1) have been demonstrated in pure albumin isolated from normal human thyroid tissue iodinated in vivo. five samples of albumin were separated from other thyroid proteins by acrylamide gel electrophoresis. after pronase hydrolysis, the content of thyroid hormones was measured chemically (t4 + t3) as well as by competitive radioactive measurement (t4) and radioimmunoassay (t3). the purity of the albumin and validity of these measurements were confirmed by differe ... | 1976 | 946147 |
| studies on the mechanism of action of potassium iodide on thyroid protein biosynthesis. | potassium iodide (ki) has been shown to have an antigoitrogenic action and to inhibit in vivo thyroid protein biosynthesis. beef thyroid slices were used to clarify further the mechanism of action of ki. incubations were performed in krebs-ringer-bicarbonate (krb) buffer under 95%o2 and 5% co2. ki caused a slight decrease in the uptake of [3h]eucine by the tissue. when labelled leucine incorporation into protein was measured it was found that 10(-6) m ki caused a marked inhibition. increasing co ... | 1976 | 946922 |
| hyperthyroidism in graves disease. current trends in management and diagnosis. | the radioimmunoassay for t3 is now widely available and is a useful diagnostic tool for hyperthyroidism, especially in t3-thyrotoxicosis. it is an essential tool in the management of hyperthyroidism that persists after treatment with normal t4 serum levels or, in euthyroid cases, with low t4 serum levels. in these conditions, it reflects the metabolic state more accurately than serum levels of t4. a promising new test is the response of radioimmunoassayable tsh to protirelin (trh) administration ... | 1976 | 946941 |
| the effects of ergocornine on tail height, spontaneous and t4-induced metamorphosis and thyroidal uptake of radioiodide in neotenic ambystoma tigrinum. | 1976 | 946962 | |
| reciprocal effects of thyroxine and triiodothyronine on their deiodination by rat tissues in vitro. | the reciprocal effects of loading doses of thyroxine (t4) or triiodothyronine (t3) on the deiodination of their 125iodine-labelled isotopes by rat muscle and liver homogenates were studied. in 21 experiments muscle homogenates deiodinated a mean 45.0% of a tracer dose of [125i]t4 and 18.0% of [125i]t3. on addition of graded amounts of nonradioactive t4 or t3 the percentual deiodination of both labelled hormones progressively declined. this effect was significantly greater in homogenates incubate ... | 1976 | 947124 |
| the deiodination of thyroxine in hyperthyroid rats as determined by renal clearance of iodide. | other investigators have reported that whole body clearance of thyroxine (t4) is increased in hyperthyroid rats isotopically equilibrated with radioactive t4, using the 24 h post-injection serum t4 concentration in the clearance calculation. data from this laboratory indicate that serum t4 concentration is lowest at this point yielding falsely high clearance values, particularly when high doses of t4 are injected. to investigate this problem further, two types of experiments were performed. firs ... | 1976 | 947283 |
| paraplegia following transurethral surgery. | we have presented a case of paraplegia following spinal anaesthesia administered for a transurethral prostatic resection. further investigation revealed a metastatic peridural compressive lesion at the level of t4. we have discussed some of the contributory factors involved, chiefly the lumbar puncture (perimedullary vascular engorgement, alterations in c.s.f. dynamics) and the possible role of intra-operative water intoxication (swelling of spinal cord cells). | 1976 | 947506 |
| radioimmunoassay for 3,3',5'-triiodothyronine (reverse t3, r-t3) in unextracted human serum. | highly specific antibodies against 3,3',5'-triiodothyronine (reverse t3, r-t3) have been produced in rabbits. the crossreaction with t4 is about 0.05%. a radioimmunoassay for r-t3 in unextracted serum was developed. ans is used for blocking the binding of tracer and endogenous r-t3 to tbg. the sensitivity to the assay is 0.06 ng/ml plasma. the mean normal r-t3 concentration is 0.20 ng/ml. thyrotoxic patients show elevated levels; in most hypothyroid patients r-t3 concentrations are below the det ... | 1976 | 947601 |
| a simple and rapid thyroxine radioimmunoassy (t4-ria) in unextracted human serum; a comparison of t4-ria and t4 displacement assay, t4(d), in normal and pathologic sera. | a simple, rapid and accurate thyroxine radioimmunoassay (t4-ria) in unextracted serum or plasma has been described, and for comparison t4 determinations have also been made by a t4(d) procedure using abbott tetrasorb kits. t4-ria procedure basically involved denaturation of serum to dissociate t4-protein bond, and t4 released was allowed to react with [125i]t4-labeled t4 antiserum elicited by immunizing rabbits against bovine thyroglobulin. the displaced unbound [125i]t4 was rapidly taken up by ... | 1976 | 947623 |
| the specificity requirements of bacteriophage t4 lysozyme. | a series of bacterial cell wall glycopeptides of low molecular weight and cell wall nucleotide precursors have been tested for their inhibitory action on the digestion by t4 lysozyme of a radioactively labeled linear uncrosslinked peptidoglycan. the disaccharide-peptides glcnac-murnac-l-ala-d-glu(a2pm) (c5) and glcnac-murnac-l-ala-d-glu(a2pm-d-ala) (c6) as well as the monosaccharide-peptide murnac-l-ala-d-glu(a2pm) were found to be good competitive inhibitors (with similar ki values) whereas th ... | 1976 | 947753 |
| changes in serum triiodothyronine, thyroxine, and thyrotropin during treatment with thyroxine in severe primary hypothyroidism. | studies were performed on 40 patients with severe primary hypothyroidism, during treatment with varying doses of t4. therapy was initiated with 50 mug/day and was continued for at least 2 months. subsequent repeated increases of 25 mug/day were continued for at least 2 months until the serum tsh level was less than 10 muu/ml. measurements of serum t4 and t3, rt3u, and serum tsh were carried out at monthly intervals. trh tests were performed after tsh levels of less than 10 muu/ml had been attain ... | 1976 | 947930 |
| plasma thyroxine concentrations in ewes and their fetuses during the last six weeks of pregnancy. | changes in plasma thyroxine (t4) concentrations were followed in 27 fetal sheep after surgical implantation of catheters. fourteen days were required before stable concentrations of t4 were achieved, whether surgery was performed between 90 and 96 days or 109 and 120 days gestation. twenty-three fetuses were followed to birth, and during the last four days the t4 concentrations showed no change in 11 fetuses and a significant decrease in the other 12. birth occurred between 142 and 157 days gest ... | 1976 | 951516 |
| increased peroxidase activity in pendred's syndrome with hypothyroidism. | an 8-year-old boy with goiter and bilateral nerve deafness had a 46% discharge of radioiodine after thiocyanate administration. he was clinically euthyroid. although the serum total t4 was low (2.4 mug/100 ml) and tsh was significantly high (181 muu/ml), the serum total t3 was normal (152 ng/100ml). it was considered that the increased release of tsh by the feedback mechanism in response to the low t4 resulted in a quite normal level of serum t3. the thyroid gland demonstrated a low stable iodin ... | 1976 | 951708 |
| a screening test for thyroid function. | the achilles tendon reflex half relaxation time measurement (art) has been used by many physicians both as a diagnostic test and for the assessment of progress in thyroid gland malfunction. reference is made to some results obtained in melbourne and in other countries using different methods of measurement of the art for these purposes. in a series of 2064 patients referred to the shepherd foundation centre, the achilles tendon reflex half relaxation time was measured by means of the smi reflexo ... | 1976 | 952638 |
| triiodothyronine and thyroxine interrelationships in health and disease. | with the recent development of radioimmunoassay techniques for the measurement of serum triiodothyronine (t3) concentration, new concepts have arisen regarding the biologic role of t3 in health and disease and its interrelationships with thyroxine (t4). an awareness of the influence of clinical conditions that affect binding of thyroid hormone to plasma proteins is required in the interpretation of moderately increased or decreased serum t3 values. hormone preparations containing t3 may produce ... | 1976 | 953904 |
| correlation between structural transformation and cleavage of the major head protein of t4 bacteriophage. | we have studied the maturation of t4 polyheads, the aberrant tubular structures related to the capsid of t4 bacteriophage. conditions have been found under which more than 95% of the major head protein (p23) undergoes the same cleavage that occurs during development of the normal capsid. the concomitant structural changes in the polyheads have been followed using electron microscope image filtering techniques. as a result of the cleavage, a radical transformation of the hexagonal lattice occurs, ... | 1976 | 954078 |
| the assessment of 125i treatment of thyrotoxicosis. | 257 patients have been reviewed 1-5 years (mean 3 years 2 months) after receiving one of five dose regimes of 125i for thyrotoxicosis. the cumulative incidence of hypothyroidism was 34% and of persistent thyrotoxicosis 17%. the group receiving doses between 351 and 500 muci/g had the highest proportion of euthyroid patients (65%) with the lowest requirement for repeat therapy (46%). in the euthyroid patients, increasing dose of 125i was associated with progressive decline in mean thyroxine (t4) ... | 1976 | 954217 |
| characterization of t4 mutants that partially suppress the inability of t4rii to grow in lambda lysogens. | in the course of isolating viable t4 deletions that affect plaque morphology (homyk and weil 1974), two closely linked point mutant, sip1 and sip2, were obtained. they map between genes t and 52, cause a reduction in plaque size and burst size, and partially suppress the lethality of rii mutants for growth in lambda lysogens. the characteristics demonstrate that sip1 and sip2 are similar to mutants previously reported by freedman and brenner(1972). in addition, d. hall (personal communication) h ... | 1976 | 955402 |