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l-theanine attenuates abstinence signs in morphine-dependent rhesus monkeys and elicits anxiolytic-like activity in mice.l-theanine, 2-amino-4-(ethylcarbamoyl) butyric acid, an amino acid found in green tea (camellia sinensis), is sold in the united states as a dietary supplement to reduce stress and improve cognition and mood. the observations that l-theanine has been shown to inhibit caffeine's stimulatory effects and that caffeine produces precipitated withdrawal signs in opioid-addicted monkeys and some opioid withdrawal signs in some normal monkeys, suggest that l-theanine may suppress opioid withdrawal signs ...201222935630
growth inhibition of walker carcinosarcoma 256 with alcoholic extract of green tea leaves (camellia sinensis).to evaluate the antitumor activity of alcoholic extracts of green tea (camella sinensis).201222936089
antioxidant capacity of teas and herbal infusions: polarographic assessment.hydrogen peroxide scavenging (hps) activity of unfermented (green, yellow, and white), partially fermented (oolong), and completely fermented (black) tea ( camellia sinensis ), maté ( ilex paraguariensis ), and various herbal infusions, as well as individual compounds (flavan-3-ols, flavonols, cinnamic and benzoic acids, and methylxanthines), was assessed by recently developed direct current (dc) polarographic assay. correlations of tea and herbal infusion hps activity with total phenolic conten ...201222950743
effects of addition of egg ovotransferrin-derived peptides on the oxygen radical absorbance capacity of different teas.ovotransferrin-derived peptides showed synergistic effects with vitamin c, epigallocatechin gallate (egcg), and caffeic acid, but not quercetin in our previous report. in this study, we further investigated the interactions between ovotransferrin-derived peptides and teas, based on the oxygen radical absorbance capacity (orac) assay. our results showed that there was no significant difference in orac values among green, oolong, and black teas. for all the samples, 80% methanol extracts possessed ...201222953899
coffee and tea consumption and risk of lung cancer: a dose-response analysis of observational studies.results from the recent meta-analysis suggested a favorable effect of green tea consumption and risk of lung cancer, while no significant association was found between black tea consumption and risk of lung cancer. besides, a significantly positive association was found between coffee consumption and risk of lung cancer. however, the relationship of green tea and coffee consumption is unclear. thus the dose-response relationship was assessed by restricted cubic spline model and multivariate rand ...201222964413
new phenolic compounds from camellia sinensis l. fermented leaves.two new phenolic compounds (1 and 2, named as teasperol and teasperin, respectively) were isolated from fermented tea (camellia sinensis l.) products, together with known phenolic compounds. teasperol (1) was isolated from chinese traditional post-fermented tea leaves (liu-pao tea) and teasperin (2) was from a japanese tea product which was selectively fermented with aspergillus sp. the chemical structures of 1 and 2 were elucidated based on the analyses of their spectroscopic data.201322972630
incompatibility between propericiazine oral solution and tea-based drink.here, we studied the incompatibility between an oral solution of propericiazine (pcz), an antipsychotic drug, and various commercially available bottled tea-based drinks. when 0.5 ml of the pcz oral solution (10 mg/ml) was mixed with 16.5 ml of a tea-based drink (such as green tea, oolong tea, and black tea), the residual pcz content declined to approximately 50% in some mixed solutions. after mixing with other tea-based drinks, the residual pcz content declined to approximately 30%, while in ot ...201222976331
characterisation of volatile and non-volatile metabolites in etiolated leaves of tea (camellia sinensis) plants in the dark.aroma is an essential factor affecting the quality of tea (camellia sinensis) products. while changes of volatile compounds during tea manufacturing have been intensively studied, the effect of environmental factors on volatile contents of fresh tea leaves has received less attention. we found that c. sinensis var. yabukita kept in darkness by shading treatment for 3 weeks developed etiolated leaves with significantly increased levels of volatiles, especially volatile phenylpropanoids/benzenoids ...201222980801
planar solid phase extraction clean-up for pesticide residue analysis in tea by liquid chromatography-mass spectrometry.efficient clean-up is indispensable for preventing matrix effects in multi-residue analysis of pesticides in food by liquid and gas chromatography (lc and gc) coupled to mass spectrometry (ms). high-throughput planar solid phase extraction (htpspe) was recently introduced as a new clean-up concept in residue analysis of pesticides in fruit and vegetables (c. oellig, w. schwack, 2011 [45]). thin-layer chromatography (tlc) was used to completely separate pesticides from matrix compounds and to foc ...201222981507
improvement of tea leaves fermentation through pectinases.the pectinase enzymes isolated from aspergillus niger, byssochlamys fulva and mucor circinelloides were used for fermentation of tea leaves from camellia sinensis plant. the use of partially purified enzymes from aspergillus niger and mucor circinelloides resulted in significant (p < 0.001) increase in the phenolic compounds, hence, improvement in tea quality. maximum increase in phenolic compounds was found in tea leaves treated with partially purified polygalacturonase (pgase) from mucor circi ...201222982636
color stability of siloranes versus methacrylate-based composites after immersion in staining solutions.the purpose of this study was to determine, by using a spectrophotometer device, the color stability of silorane in comparison with four methacrylate-based composites after being immersed in different staining solutions such as coffee, black tea, red wine, orange juice, and coke, and distilled water as control group.201322993112
effects of the medicinal plants curcuma zedoaria and camellia sinensis on halitosis control.volatile sulphur compounds (vsc) are the gases mainly responsible for halitosis (bad breath). the aim of this research was to evaluate the effects of medicinal plants on halitosis control. two commonly used plants were tested: curcuma zedoaria and camellia sinensis (green tea). these plants were prepared as an aqueous solution and used as mouthwashes, compared with a standard mouthwash of 0.12% chlorhexidine gluconate and a placebo (water). the experiment was conducted with 30 volunteers from th ...201323019084
antimicrobial activities of tocklai vegetative tea clones.thirty-one tocklai vegetative (tv) tea clones contained caffeine and total catechin 44.39 and 227.55 mg/g dry weight of leaves, respectively. the (-)-epigallocatechin gallate (egcg) was the most abundant (109.60 mg/g) followed by -(-)-epigallocatechin (egc, 44.54 mg/g), (-)-epicatechin gallate (ecg, 41.74 mg/g), (-)-epicatechin (ec, 27.42 mg/g) and +catechin (4.25 mg/g). total catechins were highest in tv 20 (509.7 mg/g) and lowest in tv 6 (71.7 mg/g). the tea clones that contain high level of t ...201123024406
influence of exogenous pigmentation on the optical properties of orthodontic elastic ligatures.the aim of this study was to assess the optical properties of orthodontic elastic ligatures under the influence of exogenous pigments contained in the daily diet.201523032209
impedance study of tea with added taste compounds using conducting polymer and metal electrodes.in this study the sensing capabilities of a combination of metals and conducting polymer sensing/working electrodes for tea liquor prepared by addition of different compounds using an impedance mode in frequency range 1 hz-100 khz at 0.1 v potential has been carried out. classification of six different tea liquor samples made by dissolving various compounds (black tea liquor + raw milk from milkman), (black tea liquor + sweetened clove syrup), (black tea liquor + sweetened ginger syrup), (black ...201223035436
black tea: chemical analysis and stability.tea is the most popular flavored and functional drink worldwide. the nutritional value of tea is mostly from the tea polyphenols that are reported to possess a broad spectrum of biological activities, including anti-oxidant properties, reduction of various cancers, inhibition of inflammation, and protective effects against diabetes, hyperlipidemia and obesity. tea polyphenols include catechins and gallic acid in green and white teas, and theaflavins and thearubigins as well as other catechin pol ...201323037977
short-term effects of polyphenol-rich black tea on blood pressure in men and women.there is increasing evidence that black tea polyphenols contribute to vascular health. we have recently shown that regular ingestion of polyphenol-rich black tea over 6 months results in lower systolic and diastolic blood pressure. however, the time course of these effects remains unclear. therefore, our objective was to determine if short-term effects of tea on blood pressure could contribute to longer-term benefits of regular tea consumption on blood pressure. men and women (n = 111) were recr ...201323038021
structural and docking studies of a nucleoside diphosphate kinase 1 (csndpk1) from tea [camellia sinensis (l.) o. kuntze].nucleoside diphosphate kinase (ndpk, ec 2.7.4.6) is a housekeeping gene, which functions in the general homeostasis of cellular nucleoside triphosphate (ntp) pools. among the various ndpk isoforms, cytosolic ndpk1 has been shown to be the main ndpk isoform in plants, accounting for more than 70 % of total ndpk activity in plants. for the first time, a full-length cdna (697 bp), designated as csndpk1 was cloned from tea leaves and consisted of a 448-bp open reading frame (orf) encoding a 147-amin ...201223054822
mechanism of hydrolysis of native and cooked starches from different botanical sources in the presence of tea extracts.a series of experiments were conducted to highlight the mechanism of inhibition of hydrolysis and differences in hydrolysis among starches from different sources in the presence of green or black tea extract. the first experiment showed that black tea extract was more effective at reducing final viscosity for all starches. the second experiment showed that black tea was more effective at inhibiting starch hydrolysis compared to green tea when starch, tea extract, and pancreatin were added at the ...201223057852
aflatoxin-induced biochemical changes in liver of mice and its mitigation by black tea extract.aflatoxin belongs to the class of naturally occurring mycotoxins, food contaminants having potent carcinogenicity. we have evaluated the ameliorative role of black tea extract on aflatoxin-induced biochemical changes in the liver of albino male mice. adult male mice were orally administered with 750 and 1500 pg of aflatoxin in 0.2 ml olive oil/kg b.w./day for 30 days. oral administration of aflatoxin caused, as compared with controls, significant, dose-dependent reduction in dna, rna, protein an ...201223061280
suppressive subtractive hybridization approach revealed differential expression of hypersensitive response and reactive oxygen species production genes in tea (camellia sinensis (l.) o. kuntze) leaves during pestalotiopsis thea infection.tea (camellia sinensis (l.) o. kuntze) is an economically important plant cultivated for its leaves. infection of pestalotiopsis theae in leaves causes gray blight disease and enormous loss to the tea industry. we used suppressive subtractive hybridization (ssh) technique to unravel the differential gene expression pattern during gray blight disease development in tea. complementary dna from p. theae-infected and uninfected leaves of disease tolerant cultivar upasi-10 was used as tester and driv ...201223065401
structural identification of theaflavin trigallate and tetragallate from black tea using liquid chromatography/electrospray ionization tandem mass spectrometry.black tea contains two major pigments, theaflavins and thearubigins. these polyphenols have been associated with certain health benefits including prevention of heart disease and cancer. elucidating and characterizing the structural aspects of thearubigins, the most abundant pigment in black tea, has been a challenge for many years. therefore further studies of black tea polyphenols must be conducted in effort to solve this thearubigin dispute. in the present study, black tea extract was found t ...201223066878
anti-infective properties of epigallocatechin-3-gallate (egcg), a component of green tea.the consumption of green tea (camellia sinensis) has been shown to have many physiological and pharmacological health benefits. in the past two decades several studies have reported that epigallocatechin-3-gallate (egcg), the main constituent of green tea, has anti-infective properties. antiviral activities of egcg with different modes of action have been demonstrated on diverse families of viruses, such as retroviridae, orthomyxoviridae and flaviviridae and include important human pathogens lik ...201323072320
application of enzymes in the production of rtd black tea beverages: a review.ready-to-drink (rtd) tea is a popular beverage in many countries. instability due to development of haze and formation of tea cream is the common problem faced in the production of rtd black tea beverages. thus decreaming is an important step in the process to meet the cold stability requirements of the product. enzymatic decreaming approaches overcome some of the disadvantages associated with other conventional decreaming methods such as cold water extraction, chill decreaming, chemical stabili ...201323072532
an improved plant leaf protein extraction method for high resolution two-dimensional polyacrylamide gel electrophoresis and comparative proteomics.we report here a simple and universally applicable protocol for extracting high quality proteins from plant leaf tissues. the protocol provides improved resolution and reproducibility of two-dimensional polyacrylamide gel electrophoresis (2-de) and reduces the time required to analyze samples. partitioning rubisco by polyethylene glycol (peg) fractionation provides clearer detection of low-abundance proteins. co-extraction of interfering substances increases the sample conductivity, which result ...201323072551
gut microbial metabolism of polyphenols from black tea and red wine/grape juice is source-specific and colon-region dependent.the colonic microbial degradation of a polyphenol-rich black tea extract (bte) and red wine/grape juice extract (rwge) was compared in a five-stage in vitro gastrointestinal model (twinshime). microbial metabolism of bte and rwge polyphenols in the twinshime was studied subsequently in single- and continuous-dose experiments. a combination of liquid or gas chromatography with mass spectrometry (lc-ms or gc-ms) and nmr-based metabolic profiling was used to measure selected parent polyphenols, the ...201223072624
in vitro screening of medicinal plants used in mexico as antidiabetics with glucosidase and lipase inhibitory activities.this work shows the inhibitory effect on glucosidase and lipase enzymes of 23 medicinal plants described as traditional treatments for diabetes in several mexican sources. hydroalcoholic extracts of selected plants were evaluated at 1 mg/ml for glucosidase and 0.25 mg/ml for lipase inhibitory activities, respectively. camellia sinensis, acarbose, and orlistat were used as positive controls. dose-response curves were done with the most active species. sixty percent of all tested extracts inhibite ...201223082084
overexpression of camellia sinensis thaumatin-like protein, cstlp in potato confers enhanced resistance to macrophomina phaseolina and phytophthora infestans infection.thaumatin-like proteins (tlps), a class of pathogenesis related proteins are induced in response to pathogens and exhibit antifungal property when overexpressed in transgenic plants. in the present study, we have raised transgenic potato plants overexpressing tlp gene of camellia sinensis (cstlp). fungal resistance assays of transgenic potato elucidated the potential role of cstlp in imparting tolerance to fungal pathogens, macrophomina phaseolina (necrotrophic) and phytophthora infestans (hemi- ...201323086453
l-theanine administration results in neuroprotection and prevents glutamate receptor agonist-mediated injury in the rat model of cerebral ischemia-reperfusion.while the neuroprotective effect of green tea (camellia sinensis) might be explained by the presence of amino acid l-theanine in the tea leaves, it is not known whether postischemic administration of l-theanine could also provide neuroprotection. in the present study, we investigated the neuroprotective effect of l-theanine (1 and 4 mg/kg) administered at 3, 12, and 24 h after reperfusion in the rat model of stroke. we also studied the effect of l-theanine on brain injury caused by exogenous adm ...201323097345
compensation for matrix effects in the gas chromatography-mass spectrometry analysis of 186 pesticides in tea matrices using analyte protectants.a gas chromatography-mass spectrometry (gc-ms) analytical method was developed for simultaneously determining 186 pesticides in tea matrices using analyte protectants to counteract the matrix-induced effect. the matrix effects were evaluated for green, oolong and black tea, representing unfermented, partially fermented and completely fermented teas respectively and depending on the type of tea, 72%, 94% and 94% of the pesticides presented strong response enhancement effect. several analyte prote ...201223102633
antioxidant and lipid lowering activities of indian black tea.indian black tea; ctc leaf and dust, produced by tata tea limited, kolkata, (india) was studiedin vitro as potential scavenger of oxygen free radicals. super oxide anions were generated in a system containing xanthine-xanthine oxidase (enzymic system) and by nadh- phenozine methosulphate (non enzymic system). anions were assayed in terms of uric acid formation and reduction of nitroblue tetrazolium salt, which were shown to be suppressed by tea extracts. extracts from both leaf and dust also inh ...200523105515
polyphenols extracted from black tea (camellia sinensis) residue by hot-compressed water and their inhibitory effect on pancreatic lipase in vitro.polyphenols, retained in black tea wastes following the commercial production of tea beverages, represent an underutilized resource. the purpose of this study was to investigate the potential use of hot-compressed water (hcw) for the extraction of pancreatic lipase-inhibiting polyphenols from black tea residues. black tea residues were treated with hcw at 10 °c intervals, from 100 to 200 °c. the resulting extracts were analyzed using high-performance liquid chromatography-mass spectrometry and a ...201223106349
tea consumption reduces ovarian cancer risk.to ascertain the relationship between tea drinking and the risk of ovarian cancer among southern chinese women, a case-control study was conducted in southern china during 2006-2008.201323107758
comparison of catechin profiles in human plasma and urine after single dosing and regular intake of green tea (camellia sinensis).green tea (camellia sinensis) catechin profiles in plasma and urine following single dosing and regular ingestion of green tea are not clear. we performed a placebo-controlled intervention study with sixteen healthy volunteers to determine changes in total and free catechins after a single dose and following 1 week of twice-daily green tea. blood and urine samples were collected before (fasting) and after (60 and 120 min for blood; 90 and 180 min for urine) drinking 200 ml of 1.5% (w/v) green te ...201323110850
determination and estimation of pharmacokinetic profile of caffeine in form of extract of green tea leaves and its analogy with synthetic form.the aim of the study was to formulate and investigate the pharmacokinetic parameters for the tablets of herbal extract of caffeine with comparison to synthetic formulation. the tablets of the aqueous herbal extract of leaves of camellia sinensis and synthetic caffeine were formulated by wet granulation technique. the hplc and hptlc were applied as analytical tools for estimation of caffeine. the batches of formulation (b1 to b7) were subjected for various pre and post-formulation studies. the ph ...201123112399
identification of flavonoids with trypsin inhibitory activity extracted from orange peel and green tea leaves.orange peel (citrus sinensis) and green tea (camellia sinensis) leaves, rich sources of food flavonoids, were analyzed for their trypsin inhibitory potential. hesperetin, rutin and hesperidin from orange peel, and catechin from green tea leaves, were isolated and their chemical structures were analyzed. all four compounds were evaluated for their trypsin inhibitory potential.201323124490
pesticide analysis in teas and chamomile by liquid chromatography and gas chromatography tandem mass spectrometry using a modified quechers method: validation and pilot survey in real samples.this paper presents the validation of a modified quechers method in four matrices - green tea, red tea, black tea and chamomile. the experiments were carried out using blank samples spiked with a solution of 86 pesticides (insecticides, fungicides and herbicides) at four levels - 10, 25, 50 and 100 μg/kg. the samples were extracted according to the citrate quechers protocol; however, to reduce the amount of coextracted matrix compounds, calcium chloride was employed instead of magnesium sulphate ...201223127810
effect of green tea (camellia sinensis) consumption on the components of metabolic syndrome in elderly.to evaluate the effect of the consumption of green tea on components of ms in the elderly.201223131813
purification and characterization of a novel galloyltransferase involved in catechin galloylation in the tea plant (camellia sinensis).catechins (flavan-3-ols), the most important secondary metabolites in the tea plant, have positive effects on human health and are crucial in defense against pathogens of the tea plant. the aim of this study was to elucidate the biosynthetic pathway of galloylated catechins in the tea plant. the results suggested that galloylated catechins were biosynthesized via 1-o-glucose ester-dependent two-step reactions by acyltransferases, which involved two enzymes, udp-glucose:galloyl-1-o-β-d-glucosyltr ...201223132863
expressed sequence tags from organ-specific cdna libraries of tea (camellia sinensis) and polymorphisms and transferability of est-ssrs across camellia species.tea is one of the most popular beverages in the world and the tea plant, camellia sinensis (l.) o. kuntze, is an important crop in many countries. to increase the amount of genomic information available for c. sinensis, we constructed seven cdna libraries from various organs and used these to generate expressed sequence tags (ests). a total of 17,458 ests were generated and assembled into 5,262 unigenes. about 50% of the unigenes were assigned annotations by gene ontology. some were homologous t ...201223136530
relationships between black tea consumption and key health indicators in the world: an ecological study.the aim of this study was to investigate potential statistical relationships between black tea consumption and key health indicators in the world. the research question is: does tea consumption is correlated with one or more epidemiological indicators?201223138107
evaluation of camellia sinensis catechins as a swine antimicrobial feed additive that does not cause antibiotic resistance.antimicrobial growth promoters (agps) have been banned and phased out because their use has been linked to the emergence and spread of antibiotic-resistant pathogens; however, the ban has had a marked impact on livestock production, and feed additive alternatives to agps are required. we focused on green tea leaves as potential alternatives to agps because they contain significant amounts of polyphenol catechins, which have antivirus and antimicrobial effects. we examined cross-resistance betwee ...201323138151
integrating satellite images and spectroscopy to measuring green and black tea quality.this study quantifies the effects of green leaf and black tea parameters that influence tea quality in northeast india. it was motivated by a decline in tea quality that is of concern to tea growers. the rationale of the study is to identify the different parameters that have a significant influence on liquor brightness and other variables measuring tea quality. here, we investigate several methods for estimating tea quality based on tea quality data, near infrared spectroscopy and remotely sens ...201123140747
immunohistochemical localization of caffeine in young camellia sinensis (l.) o. kuntze (tea) leaves.the anatomical localization of caffeine within young camellia sinensis leaves was investigated using immunohistochemical methods and confocal scanning laser microscopy. preliminary fixation experiments were conducted with young c. sinensis leaves to determine which fixation procedure retained caffeine the best as determined by high-performance liquid chromatography analysis. high pressure freezing, freeze substitution, and embedding in resin was deemed the best protocol as it retained most of th ...201323143222
tea creaming in nonfermented teas from camellia sinensis and ilex vomitoria.tea creaming is the development of a cloudy or hazy appearance in tea and ready-to-drink tea products on cooling and is highly undesirable in the tea beverage industry. commonly associated with fermented black or oolong teas, the objective of this study was to investigate the physicochemical mechanism of the formation of tea cream in nonfermented green tea (camellia sinensis) and a caffeine-containing botanical tea from yaupon holly (ilex vomitoria) that is free of catechin-based polyphenolics. ...201223148671
influence of exogenously applied abscisic acid on carotenoid content and water uptake in flowers of the tea plant (camellia sinensis).carotenoids are a major class of plant pigments and fulfill many functions in different organisms that either produce or consume them. although the color of the stamina of tea (camellia sinensis) flowers is clearly due to the presence of carotenoids, the carotenoid profile and content remain to be discovered.201323152164
[mechanisms of cerebral protection from ischemia by tea constituents].epidemiological and clinical studies in recent years have shown that regular consumption of green or black tea significantly reduces the risk of cardiovascular diseases, including ischemic stroke. this review presents the clinical and experimental studies of the antiatherogenic, antiplatelet, antioxidant, antiinflammatory and other mechanisms of action of tea and substances in its composition. effects of tea and its components, are described after long-term, and a short-term consumption. the rol ...201223155618
rapid and simultaneous determination of antioxidant markers and caffeine in commercial teas and dietary supplements by hplc-dad.a simple and fast reverse-phase high-performance liquid chromatography procedure coupled with photodiode array detector (rp-hplc-dad) was developed and validated for the analysis of major catechins, proanthocyanidin (procyanidin b2) and caffeine in 25 different natural complex matrices containing camellia sinensis l. and/or grape seed extracts, two popular plant extracts that have been widely used as natural antioxidants in various food and beverage applications. using an isocratic elution syste ...201223158288
phenolic promiscuity in the cell nucleus--epigallocatechingallate (egcg) and theaflavin-3,3'-digallate from green and black tea bind to model cell nuclear structures including histone proteins, double stranded dna and telomeric quadruplex dna.flavanols from tea have been reported to accumulate in the cell nucleus in considerable concentrations. the nature of this phenomenon, which could provide novel approaches in understanding the well-known beneficial health effects of tea phenols, is investigated in this contribution. the interaction between epigallocatechin gallate (egcg) from green tea and a selection of theaflavins from black tea with selected cell nuclear structures such as model histone proteins, double stranded dna and quadr ...201323172122
the r2r3-myb, bhlh, wd40, and related transcription factors in flavonoid biosynthesis.r2r3-myb, bhlh, and wd40 proteins have been shown to control multiple enzymatic steps in the biosynthetic pathway responsible for the production of flavonoids, important secondary metabolites in camellia sinensis. few related transcription factor genes have been documented. the presence of r2r3-myb, bhlh, and wd40 were statistically and bioinformatically analyzed on 127,094 c. sinensis transcriptome unigenes, resulting in identification of 73, 49, and 134 genes, respectively. c. sinensis phyloge ...201323184474
the in vivo antioxidant and antifibrotic properties of green tea (camellia sinensis, theaceae).the in vivo antioxidant and antifibrotic properties of green tea (camellia sinensis, theaceae) were investigated with a study of carbon tetrachloride (ccl(4))-induced oxidative stress and hepatic fibrosis in male icr mice. oral administration of green tea extract at doses of 125, 625 and 1250 mg/kg for 8 weeks significantly reduced (p<0.05) the levels of thiobarbituric acid-reactive substances (tbars) and protein carbonyls in the liver by at least 28% compared with that was induced by ccl(4) (1 ...201323194532
identification of genes involved in indole-3-butyric acid-induced adventitious root formation in nodal cuttings of camellia sinensis (l.) by suppression subtractive hybridization.the plant hormone auxin plays a key role in adventitious rooting. to increase our understanding of genes involved in adventitious root formation, we identified transcripts differentially expressed in single nodal cuttings of camellia sinensis treated with or without indole-3-butyric acid (iba) by suppressive subtractive hybridization (ssh). a total of 77 differentially expressed transcripts, including 70 up-regulated and 7 down-regulated sequences, were identified in tea cuttings under iba treat ...201323201417
upregulation of phase ii enzymes through phytochemical activation of nrf2 protects cardiomyocytes against oxidant stress.increased production of reactive oxygen species has been implicated in the pathogenesis of cardiovascular disease (cvd), and enhanced endogenous antioxidants have been proposed as a mechanism for regulating redox balance. nuclear factor (erythroid-derived 2)-like 2 (nrf2) is a transcriptional regulator of phase ii antioxidant enzymes, and activation of nrf2 has been suggested to be an important step in attenuating oxidative stress associated with cvd. a well-defined combination of five widely st ...201323201694
effect of beverages on bovine dental enamel subjected to erosive challenge with hydrochloric acid.this study evaluated by an in vitro model the effect of beverages on dental enamel previously subjected to erosive challenge with hydrochloric acid. the factor under study was the type of beverage, in five levels: sprite® zero low-calorie soda lime (positive control), parmalat® ultra high temperature (uht) milk, ades® original soymilk, leão® ice tea zero ready-to-drink low-calorie peach-flavored black teaand prata® natural mineral water (negative control). seventy-five bovine enamel specimens we ...201223207851
tannic acid inhibits staphylococcus aureus surface colonization in an isaa-dependent manner.staphylococcus aureus is a human commensal and pathogen that is capable of forming biofilms on a variety of host tissues and implanted medical devices. biofilm-associated infections resist antimicrobial chemotherapy and attack from the host immune system, making these infections particularly difficult to treat. in order to gain insight into environmental conditions that influence s. aureus biofilm development, we screened a library of small molecules for the ability to inhibit s. aureus biofilm ...201323208606
the pentacyclic triterpenoids in herbal medicines and their pharmacological activities in diabetes and diabetic complications.pentacyclic triterpenoids including the oleanane, ursane and lupane groups are widely distributed in many medicinal plants, such as glycyrrhiza species, gymnema species, centella asiatica, camellia sinensis, crataegus species and olea europaea, which are commonly used in traditional medicine for the treatment of diabetes and diabetic complications. a large number of bioactive pentacyclic triterpenoids, such as oleanolic acid, glycyrrhizin, glycyrrhetinic acid, ursolic acid, betulin, betulinic ac ...201323210780
black tea extract: a supplementary antioxidant in radiation-induced damage to dna and normal lymphocytes.myriad research has contributed significantly toward the understanding and identification of health benefits stemming from tea polyphenols and many other naturally occurring flavonoids present in fruits and vegetables. these flavonoids are known to mitigate reactive oxygen species-induced damage by scavenging them. in this study, hot-water black tea extract rich in flavonoids is evaluated as a supplementary antioxidant. the antioxidant efficacy of black tea extract was investigated by evaluating ...201223216640
black tea high-molecular-weight polyphenol increases the motility of sea urchin sperm by activating mitochondrial respiration.mitochondria activation factor (maf) is a high-molecular-weight polyphenol purified from black tea that activates mitochondrial respiration. it increased the mitochondrial membrane potential and motility of sea urchin sperm, by up to 8%, to the same extent as sperm-activating peptides (saps) secreted by the egg. unlike saps, maf had no effect on sperm swimming behavior, suggesting that the mechanism of sperm activation by maf is different from that of saps.201223221694
green tea extract (camellia sinensis) fermented by lactobacillus fermentum attenuates alcohol-induced liver damage.here, the impact of an extract derived from green tea (camellia sinensis) and fermentation with lactobacilli fermentum strain ocs19 was explored with acute alcohol-induced liver damage. the study employed the hepg2 hepatic cell line and an in vivo murine model of liver damage. l. fermentum-fermented green tea extract (fgte) was found to possess pronounced alcohol metabolizing enzyme activity. it significantly enhanced the cell viability of hepg2 cells following of them exposure, to ethanol (p<0. ...201223221715
determination of trace and heavy metals in some commonly used medicinal herbs in ayurveda.traditionally, the herbal drugs are well established for their therapeutic benefits. depending upon their geographical sources sometimes the trace and heavy metals' content may differ, which may lead to severe toxicity. so, the toxicological and safety assessment of these herbal drugs are one of the major issues in recent days. eight different plant species including aloe vera, centella asiatica, calendula officinalis, cucumis sativus, camellia sinensis, clitoria ternatea, piper betel and tagete ...201423222691
priority pahs in orthodox black tea during manufacturing process.orthodox black tea is obtained from fresh leaves followed by withering, rolling, fermentation and drying. the presence of 16 priority polycyclic aromatic hydrocarbons (pahs) was studied in fresh leaves and at various stages of manufacturing. benzo(a)pyrene (2a: probable human carcinogen) was found in dried tea leaves only whereas, naphthalene (2b: probable human carcinogen) was present during all the stages of manufacturing. dry tea leaves showed higher content of total 16 pahs (∑pahs) about 3 a ...201323224705
volatile profile analysis and quality prediction of longjing tea (camellia sinensis) by hs-spme/gc-ms.this study aimed to analyze the volatile chemical profile of longjing tea, and further develop a prediction model for aroma quality of longjing tea based on potent odorants. a total of 21 longjing samples were analyzed by headspace solid phase microextraction (hs-spme) coupled with gas chromatography-mass spectrometry (gc-ms). pearson's linear correlation analysis and partial least square (pls) regression were applied to investigate the relationship between sensory aroma scores and the volatile ...201223225852
construction of a high-density reference linkage map of tea (camellia sinensis).a few linkage maps of tea have been constructed using pseudo-testcross theory based on dominant marker systems. however, dominant markers are not suitable as landmark markers across a wide range of materials. therefore, we developed co-dominant ssr markers from genomic dna and ests and constructed a reference map using these co-dominant markers as landmarks. a population of 54 f(1) clones derived from reciprocal crosses between 'sayamakaori' and 'kana-ck17' was used for the linkage analysis. map ...201223226087
the microbiota is essential for the generation of black tea theaflavins-derived metabolites.theaflavins including theaflavin (tf), theaflavin-3-gallate (tf3g), theaflavin-3'-gallate (tf3'g), and theaflavin-3,3'-digallate (tfdg), are the most important bioactive polyphenols in black tea. because of their poor systemic bioavailability, it is still unclear how these compounds can exert their biological functions. the objective of this study is to identify the microbial metabolites of theaflavins in mice and in humans.201223227227
molecular cloning, functional analysis of three cinnamyl alcohol dehydrogenase (cad) genes in the leaves of tea plant, camellia sinensis.cinnamyl alcohol dehydrogenase (cad; ec 1.1.1.195) is considered to be a key enzyme in lignin biosynthesis, but little was known about cads in tea plants (camellia sinensis). a full-length cdna sequence (cscad2) was isolated by suppressive subtractive hybridization (ssh) in ectropis oblique feeding-induced tea plants, and another two full-length cdna sequences (cscad1 and cscad3) were obtained from a transcriptome obtained by deep sequencing. however, they showed only 20-54% identities. phylogen ...201323228629
ni speciation in tea infusions by monolithic chromatography--icp-ms and q-tof-ms.for humans, ni is not considered to be an essential trace element. its compounds, at levels present in foodstuffs and drinks, are generally considered to be safe for consumption, but for individuals who already suffer from contact allergy to ni and may be subject to develop systemic reactions from its dietary ingestion, dietary exposure to ni must be kept under control. being the second most popular beverage, tea is a potential source of dietary ni. present knowledge on its speciation in tea inf ...201323232960
[anti-inflammatory effects of tea-flavonoids].tea flavonoids belong to the large group of polyphenols and display antioxidative, anti-inflammatory and anti-neoplastic activities. these phytochemicals are xenobiotics and are synthesized by tea plants such as camellia sinensis and camomilla recucita. these botanicals exhibit in vivo activities similar to that of biologicals which are widely used for chronic inflammatory diseases (rheumatoid arthritis, chronic inflammatory bowel disease). epigallocathechin gallate and apigenin from these plant ...201223233307
[nitrogen and phosphorus loss in different land use types and its response to environmental factors in the three gorges reservoir area].the control of agricultural non-point source pollution (agnps) is an urgent problem to be solved for the ecological environment construction in the three gorges reservoir area. we analyzed the nitrogen (n) and phosphorus (p) loss and its response to environmental factors through monitoring the nutrient loss in different land use types after returning farmland to forest. the results showed that: 1) the variability of nutrient concentration loss was strong in different land use types under differe ...201223233964
the potential of different bio adsorbents for removing phenol from its aqueous solution.the use of natural resources for the removal of phenol and phenolic compounds is being looked upon by researchers in preference to other prevailing methods. in the present study, different biosorbents, brown algae (padina pavonia), fresh water macrophyta (ceratophyllum demersum), and black tea residue, were tested as adsorbent for the removal of phenol from aqueous solutions. the optimum conditions for maximum adsorption in terms of concentration of the adsorbate and ph were identified. the resu ...201323242505
phosphorylation of hepatic amp-activated protein kinase and liver kinase b1 is increased after a single oral dose of green tea extract to mice.we have previously shown that green and black tea extracts increase the phosphorylation of amp-activated protein kinase (ampk) and hmg-coa reductase in rat hepatoma cells in culture, concomitant with a decrease in cholesterol synthesis. in the present study, we evaluated the ability of a single oral dose of green or black tea extract to promote the phosphorylation of ampk, liver kinase b1 (lkb1, an ampk-kinase), and hmg-coa reductase in mouse liver. green tea extract administered by gavage at 50 ...201223244544
synergetic downregulation of 67 kda laminin receptor by the green tea (camellia sinensis) secondary plant compound epigallocatechin gallate: a new gateway in metastasis prevention?in traditional chinese medicine, green tea is considered to have a life-prolonging effect, possibly as a result of its rich content of antioxidant tea polyphenols, and hence has the potential to prevent cancer. this study investigated the role of the major tea secondary plant compound epigallocatechin gallate (egcg) for its inhibitory effects on the metastasis-associated 67 kda laminin receptor (67lr).201223249430
[analysis of codon use features of cbf gene in camellia sinensis].cbf (c-repeat-binding factor) transcription factor exists widely in all kinds of plants. it is an important regulative factor in the process of plant resistance adversity. in this paper, camellia sinensis cbf1 gene sequence was analyzed by codon w, chips, and cusp programs online, and then compared with c. sinensis genes, genomes in other species, and cbf genes from 39 plant species. it is important to identify the codon usage of cscbf1 gene and select appropriate expression systems. the results ...201223262110
compositional analysis of leaf cuticular membranes isolated from tea plants (camellia sinensis l.).chemical constituents of cuticular membranes (cms) isolated from three tea cultivars (camellia sinensis (l.) o. kuntze cvs. yabukita, samidori and gokou) were compared. all cms from the adaxial side of the leaves showed higher accumulation of wax, cutin and polysaccharide, while those from the abaxial side were abundant in cutan, showing the adaptation of the adaxial side to abiotic stresses, such as wind and rain, in contrast to the abaxial side, which provides defence against pathogens. yabuki ...201323265489
travelling with tea: a tuckerella's tale.tuckerella japonica ehara appears strongly associated with tea (camellia sinensis (l.) kuntze, theaceae) and, due to certain cultural practices in tea production, has in fact become a world traveller, accompanying the greatly coveted tea plant as it spread across the planet. the history of tea production and culture, and its arrival in the usa, provides the backdrop for this traveller's tale. tuckerella japonica is morphologically similar to t. flabellifera miller, described from tasmania in aus ...201323266716
phytochemical analysis of the triterpenoids with cytotoxicity and qr inducing properties from the total tea seed saponin of camellia sinensis.the tea seed triterpene saponin (ts) from camellia sinensis was found to exhibit better antitumor activity in vivo in s180 implanted icr mice and qr inducing activity for hepa lclc7 cells respectively compared with the total tea seed saponin (tts), hydrolysate of the tts and tea seed flavonoid glycosides (tf). by bioassay-guided isolation, the ts fraction was separated and seven major components were purified and identified as theasaponin e1 (1), theasaponin e2 (2), theasaponin c1 (3), assamsapo ...201223266730
influence of different fermentation raw materials on pyrolyzates of pu-erh tea theabrownin by curie-point pyrolysis-gas chromatography-mass spectroscopy.in this study, pu-erh tea was prepared by solid-state fermentation of the sun-dried green tea, short-fermentation black tea, and black tea. ultraviolet-visible spectroscopy, infrared spectroscopy and curie-point pyrolysis-gas chromatography-mass spectroscopy (cp-py-gc/ms) were used to study the characteristics and chemical compositions of the tb formed in these pu-erh teas.201323270831
nitric oxide participates in cold-inhibited camellia sinensis pollen germination and tube growth partly via cgmp in vitro.nitric oxide (no) plays essential roles in many biotic and abiotic stresses in plant development procedures, including pollen tube growth. here, effects of no on cold stress inhibited pollen germination and tube growth in camellia sinensis were investigated in vitro. the no production, no synthase (nos)-like activity, cgmp content and proline (pro) accumulation upon treatment with no scavenger cptio, nos inhibitor l-nna, no donor dea nonoate, guanylate cyclase (gc) inhibitor odq or phosphodieste ...201223272244
dehydrin2 is a stress-inducible, whereas dehydrin1 is constitutively expressed but up-regulated gene under varied cues in tea [camellia sinensis (l.) o. kuntze].two expressed sequence tags with similarity to dehydrin were cloned to full-length (csdhn1 and csdhn2) through rapid amplification of cdna ends. csdhn1 and csdhn2 were 1,027 and 992 base pair long and encoded for predicted polypeptides of 260 and 201 amino acids, respectively. deduced csdhn1 protein had a s-segment and three lysine-rich consensus motifs (k-segments). the y-segment was, however, absent in the deduced csdhn1. csdhn2 had three y motifs, one s-segment and two k-segments. expression ...201323275208
isolation and characterization of histone1 gene and its promoter from tea plant (camellia sinensis).one 1.2 kbp long sequence was cloned by using pcr with primers that were designed from cdna sequence of csh1 gene (genbank: eu716314) from tea plant (camellia sinensis). according to the 1.2 kbp sequence, a 0.6 kbp sequence was isolated from tea plant genomic dna using dna walking method. sequence analysis revealed that the 1.2 kbp sequence is a csh1 gene consisting of 1 exon and 2 introns, the border of exton and intron sequences conforming to the gt-ag rule, and the 0.6 kbp sequence was found ...201323277399
nutritional status assessment of minodar residence in qazvin city, iran: vitamin d deficiency in sunshine country, a public health issue.nutrition has main effect on health or disease and results of nutrition assessment can be used in health planning of communities. therefore we aimed to conduct the nutrition assessment especially vitamin d statue of an urban region in qazvin city.201223283050
functional characterization of proanthocyanidin pathway enzymes from tea and their application for metabolic engineering.tea (camellia sinensis) is rich in specialized metabolites, especially polyphenolic proanthocyanidins (pas) and their precursors. to better understand the pa pathway in tea, we generated a complementary dna library from leaf tissue of the blister blight-resistant tea cultivar tri2043 and functionally characterized key enzymes responsible for the biosynthesis of pa precursors. structural genes encoding enzymes involved in the general phenylpropanoid/flavonoid pathway and the pa-specific branch pa ...201323288883
black tea consumption and risk of stroke in women and men.our aim was examine the association between black tea consumption and risk of total stroke and stroke types in a prospective study.201323295000
analysis of dormant bud (banjhi) specific transcriptome of tea (camellia sinensis (l.) o. kuntze) from cdna library revealed dormancy-related genes.bud dormancy is of ecological and economical interest due to its impact on tea (camellia sinensis (l.) o. kuntze) plant growth and yield. growth regulation associated with dormancy is an essential element in plant's life cycle that leads to changes in expression of large number of genes. in order to identify and provide a picture of the transcriptome profile, cdna library was constructed from dormant bud (banjhi) of tea. sequence and gene ontology analysis of 3,500 clones, in many cases, enabled ...201323315209
phenolic acid concentrations in plasma and urine from men consuming green or black tea and potential chemopreventive properties for colon cancer.tea polyphenols are metabolized by the colonic microflora yielding phenolic metabolites, which may contribute to the health benefits of tea. we determined the serum and urine concentrations of phenolic acids, hippuric acid, and polyhydroxyphenyl-γ-valerolactones during green tea (gt) and black tea (bt) administration. the effects of (-)-epigallocatechin gallate (egcg) and 3,4-dihydroxyphenylacetic acid (3,4-dhpaa) alone and in combination on bioavailability, intracellular metabolism, and antipro ...201323319439
plant food supplements with anti-inflammatory properties: a systematic review (i).plant food supplements (pfs) receive great acceptance by european consumers. however, quality and efficacy of these products remain a question of concern. the aim of this systematic review is to summarize and critically evaluate the evidence for or against the efficacy of pfs for coping inflammatory conditions by considering epidemiological and human intervention studies. the review, which consists of two parts, considers olea europea l., camellia sinensis l., vitis vinifera l., and matricaria r ...201323320910
effect of green tea extract on the treatment of dentin erosion: an in vitro study.the aim of this study was to analyze the impact of green tea on dentin erosion.201223323184
targeting ret to induce medullary thyroid cancer cell apoptosis: an antagonistic interplay between pi3k/akt and p38mapk/caspase-8 pathways.mutations in rearranged during transfection (ret) receptor tyrosine, followed by the oncogenic activation of ret kinase is responsible for the development of medullary thyroid carcinoma (mtc) that responds poorly to conventional chemotherapy. targeting ret, therefore, might be useful in tailoring surveillance of mtc patients. here we showed that theaflavins, the bioactive components of black tea, successfully induced apoptosis in human mtc cell line, tt, by inversely modulating two molecular pat ...201323329180
monitoring of pesticide residues in south indian tea.tea is the most commonly consumed beverage in the world. tea infusion is prepared by pouring boiling hot water over cured leaves of the camellia sinensis plant. such a beverage should be free from toxic chemicals. studies on the monitoring of pesticide residues in tea of south india had been carried. tea samples collected from different districts of south india were analysed for the residues of certain pesticide such as dicofol, ethion, quinalphos, hexaconazole, fenpropathrin, fenvalerate and pr ...201323341056
the effect of camellia sinensis (green tea) mouthwash on plaque-induced gingivitis: a single-blinded randomized controlled clinical trial.background and the purpose of the studycomplementary medicine received high attention during last decades. we aimed to assess the efficacy of green tea mouthwash on plaque-induced gingivitis as the most common form of periodontal disease.201223351842
the association of tea consumption with bladder cancer risk: a meta-analysis.the association between tea consumption and bladder cancer has been confirmed in several animal studies, but one epidemiological study in 2001 showed no association between them. in order to provide an accurate assessment of this, we conducted a meta-analysis on tea consumption and bladder cancer risk. studies were identified by a literature search in pubmed from january 1980 to march 2012 and the reference lists of relevant studies. random effect models were used to calculate summary relative r ...201323353620
camellia sinensis (green tea) extract attenuate acrylamide induced testicular damage in albino rats.acrylamide is a proved toxin for testicular function, found in food when heated for long period of time. green tea (camellia sinensis) is a potent antioxidant; the aim of this study was to investigate the protective effect of green tea extract against the toxic effects of acrylamide in rat testes.201423364758
effects of green tea, black tea, and coffee consumption on the risk of esophageal cancer: a systematic review and meta-analysis of observational studies.epidemiological studies regarding the associations of tea and coffee consumption with esophageal cancer (ec) risk are still inconsistent and this meta-analysis was conducted to examine these associations. pubmed, isi -web of science, china national knowledge infrastructure (cnki), and chinese vip database up to october 2011 were searched and manual search for reference lists of relevant studies were conducted. random effects model was used to pool the odds ratios (or). twenty-four case-control a ...201323368908
determination of lead, cadmium and arsenic in infusion tea cultivated in north of iran.tea is one of the most common drinks in all over the world. rapid urbanization and industrialization in recent decades has increased heavy metals in tea and other foods. in this research, heavy metal contents such as lead (pb), cadmium (cd) and arsenic (as) were determined in 105 black tea samples cultivated in guilan and mazandaran provinces in north of iran and their tea infusions. the amount of heavy metals in black tea infusions were analyzed using inductively coupled plasma atomic emission ...201223369381
adsorption kinetics of rhodamine-b on used black tea leaves.rhodamine b (rh-b) is one of the most common pollutants in the effluents of textile industries effluents in developing countries. this study was carried out to evaluate the applicability of used black tea leaves (ubtl) for the adsorptive removal of rh-b from aqueous system by investigating the adsorption kinetics in batch process. the effects of concentration and temperature on adsorption kinetics were examined. first-, second- and pseudo-second order kinetic equations were used to investigate t ...201223369452
monitoring of some pesticides residue in consumed tea in tehran market.tea is an agricultural product of the leaves, leaf buds, and internodes of various cultivars and sub-varieties of the camellia sinensis plant, processed and vulcanized using various methods. tea is a main beverage in iranian food basket so should be free from toxic elements such as pesticides residue. there is no data bank on the residue of pesticides in the consumed black tea in iran. the present study is the first attempt for monitoring of 25 pesticide residues from different chemical groups i ...201323369650
discrimination of sri lankan black teas using fluorescence spectroscopy and linear discriminant analysis.the quality of teas is currently graded using trained tea tasters, whose evaluation can sometimes be subjective. in this study the simple fluorescence-based technique of total luminescence spectroscopy (tls) in conjunction with data classification using principal component analysis (pca) was applied to discriminate between teas from 11 different sri lankan plantations. solvent extraction of the tea samples was followed by tls to record excitation-emission matrices in the excitation range 250-590 ...201323371833
comparing streptococcus mutans and lactobacillus colony count changes following green tea mouth rinse or sodium fluoride mouth rinse use in children (randomized double-blind controlled clinical trial).green tea contains phenolic compounds which could be considered as an anticariogenic agent. in addition, there has not been any significant side effect reported compared to sodium fluoride. so it seems that any comparison between the effects of green tea extract on the level of cariogenic bacteria with sodium fluoride is beneficial. the purpose of this study was to compare the effect of sodium fluoride and green tea mouth rinses on the level of salivary streptococcus mutans and lactobacillus of ...201123372597
catechins are not major components responsible for the beneficial effect of camellia sinensis on the ovarian δ-ala-d activity inhibited by cadmium.cadmium has been associated with a wide spectrum of deleterious effects on the reproductive tissues, including ovary. this investigation evaluated the protective role of camellia sinensis (green, white and red teas) in the cadmium-induced inhibition of ovarian δ-aminolevulinate dehydratase (δ-ala-d) activity in vitro and ex vivo. this study demonstrated that green and white teas restored the cow ovary δ-ala-d activity inhibited by cadmium whereas red tea had no effect in vitro. in addition, gree ...201323376781
purification, characterization and anticoagulant activity of the polysaccharides from green tea.the crude tea polysaccharides were extracted from the leaves of camellia sinensis using deionized water. the tea polysaccharides (tps) were further separated and purified by anion exchange chromatograph on deae sepharose cl-6b column to afford tps-1, tps-2, tps-3 and tps-4. the high performance gel permeation chromatograph analysis showed that the average molecular weight of polysaccharides (tps-1, tps-2 and tps-3) were 20,760, 24,230 and 250,643, respectively. tps-4 was 689, 113 and 4150, sugge ...201323399132
biological clues to potent dna-damaging activities in food and flavoring.population differences in age-related diseases and cancer could stem from differences in diet. to characterize dna strand-breaking activities in selected foods/beverages, flavorings, and some of their constituent chemicals, we used p53r cells, a cellular assay sensitive to such breaks. substances testing positive included reference chemicals: quinacrine (peak response, 51×) and etoposide (33×); flavonoids: egcg (19×), curcumin (12×), apigenin (9×), and quercetin (7×); beverages: chamomile (11×), ...201323402862
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