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fine mapping and candidate gene analysis of qfl-chr1, a fiber length qtl in cotton.a fiber length qtl, qfl-chr1, was fine mapped to a 0.9 cm interval of cotton chromosome 1. two positional candidate genes showed positive correlation between gene expression level and fiber length. prior analysis of a backcross-self mapping population derived from a cross between gossypium hirsutum l. and g. barbadense l. revealed a qtl on chromosome 1 associated with increased fiber length (qfl-chr1), which was confirmed in three independent populations of near-isogenic introgression lines (nii ...201728361363
genome-wide association study discovered candidate genes of verticillium wilt resistance in upland cotton (gossypium hirsutum l.).verticillium wilt (vw), caused by infection by verticillium dahliae, is considered one of the most yield-limiting diseases in cotton. to examine the genetic architecture of cotton vw resistance, we performed a genome-wide association study (gwas) using a panel of 299 accessions and 85,630 single-nucleotide polymorphisms (snps) detected using the specific-locus amplified fragment sequencing (slaf-seq) approach. trait-snp association analysis detected a total of 17 significant snps at p < 1.17 × 1 ...201728371164
genome-wide identification of membrane-bound fatty acid desaturase genes in gossypium hirsutum and their expressions during abiotic stress.membrane-bound fatty acid desaturases (fads) are of great importance and play multiple roles in plant growth and development. in the present study, 39 full-length fad genes, based on database searches, were identified in tetraploid upland cotton (gossypium hirsutum l.) and were phylogenetically clustered into four subfamilies. genomic localization revealed that 34 genes were mapped on 22 chromosomes, and five genes were positioned on the scaffold sequences. the fad genes of g. hirsutum in the sa ...201728374822
genome-wide identification of the mikc-type mads-box gene family in gossypium hirsutum l. unravels their roles in flowering.cotton is one of the major world oil crops. cottonseed oil meets the increasing demand of fried food, ruminant feed, and renewable bio-fuels. mads intervening keratin-like and c-terminal (mikc)-type mads-box genes encode transcription factors that have crucial roles in various plant developmental processes. nevertheless, this gene family has not been characterized, nor its functions investigated, in cotton. here, we performed a comprehensive analysis of mikc-type mads genes in the tetraploid gos ...201728382045
development and survival of spodoptera exigua (lepidoptera: noctuidae) on alternate crops in cotton cropping pattern, with implications to integrated pest management.spodoptera exigua (hübner) is a polyphagous pest that shifts its population to different hosts during its life cycle to receive nutritive advantages. therefore, demographic evaluation of alternate hosts is important for effective pest management. here, we have evaluated castor (ricinus communis l.), cauliflower (brassica oleracea l.), cotton (gossypium hirsutum l.), okra (abelmoschus esculentus l.), and spinach (spinacia oleracea l.) for growth, survival, and population development of s. exigua. ...201728383691
overexpression of cotton pyl genes in arabidopsis enhances the transgenic plant tolerance to drought stress.pyr/pyl/rcar proteins are putative abscisic acid (aba) receptors that play important roles in plant responses to biotic and abiotic stresses. in this study, 27 predicted pyl proteins were identified in cotton (gossypium hirsutum). sequence analysis showed they are conserved in structures. phylogenetic analysis showed that cotton pyl family could be categorized into three groups. yeast two-hybrid assay revealed that the ghpyl proteins selectively interacted with some ghpp2c proteins. quantitative ...201728388505
responses of neonicotinoid-resistant and susceptible frankliniella fusca life stages to multiple insecticide groups in cotton.detection of neonicotinoid resistance in populations of tobacco thrips, frankliniella fusca hinds, throughout the southeastern us has motivated an examination of alternative insecticides to control problematic infestations on seedling cotton. the objective of this study was to refine understanding of stage-specific mortality and reduced oviposition of several common insecticides (acephate, abamectin, cyantraniliprole, spinetoram, imidacloprid, imidacloprid + thiodicarb, thiamethoxam) on neonicot ...201728401732
genome-wide comparative analysis of nbs-encoding genes in four gossypium species.nucleotide binding site (nbs) genes encode a large family of disease resistance (r) proteins in plants. the availability of genomic data of the two diploid cotton species, gossypium arboreum and gossypium raimondii, and the two allotetraploid cotton species, gossypium hirsutum (tm-1) and gossypium barbadense allow for a more comprehensive and systematic comparative study of nbs-encoding genes to elucidate the mechanisms of cotton disease resistance.201728403834
the verticillium-specific protein vdscp7 localizes to the plant nucleus and modulates immunity to fungal infections.fungal pathogens secrete effector proteins to suppress plant basal defense for successful colonization. resistant plants, however, can recognize effectors by cognate r proteins to induce effector-triggered immunity (eti). by analyzing secretomes of the vascular fungal pathogen verticillium dahliae, we identified a novel secreted protein vdscp7 that targets the plant nucleus. the green fluorescent protein (gfp)-tagged vdscp7 gene with either a mutated nuclear localization signal motif or with add ...201728407259
single-base resolution methylomes of upland cotton (gossypium hirsutum l.) reveal epigenome modifications in response to drought stress.dna methylation, with a cryptic role in genome stability, gene transcription and expression, is involved in the drought response process in plants, but the complex regulatory mechanism is still largely unknown.201728407801
micrornas as reference genes for quantitative pcr in cotton.cotton (gossypium hirsutum) is the most important non-food plant in the world. studies concerning the fiber quality and plant fitness of cotton at molecular level depend on high sensitive and reproducible gene-expression assays. however, only a few reports have described genes suitable for normalizing gene expression data. in this study, we report for the first time that micrornas (mirnas) are reliable reference genes (rgs) for cotton gene expression normalization in quantitative real-time rever ...201728414734
characterization of two tt2-type myb transcription factors regulating proanthocyanidin biosynthesis in tetraploid cotton, gossypium hirsutum.two tt2-type myb transcription factors identified from tetraploid cotton are involved in regulating proanthocyanidin biosynthesis, providing new strategies for engineering condensed tannins in crops. proanthocyanidins (pas), also known as condensed tannins, are important secondary metabolites involved in stress resistance in plants, and are health supplements that help to reduce cholesterol levels. as one of the most widely grown crops in the world, cotton provides the majority of natural fabric ...201728421329
a genome-scale analysis of the pin gene family reveals its functions in cotton fiber development.the pin-formed (pin) protein, the most important polar auxin transporter, plays a critical role in the distribution of auxin and controls multiple biological processes. however, characterizations and functions of this gene family have not been identified in cotton. here, we identified the pin family in gossypium hirsutum, gossypium arboreum, and gossypium raimondii. this gene family was divided into seven subgroups. a chromosomal distribution analysis showed that ghpin genes were evenly distribu ...201728424725
potassium deficiency affects the carbon-nitrogen balance in cotton leaves.potassium (k) plays important roles in the metabolism of carbon (c) and nitrogen (n), but studies of k deficiency affecting c-n balance are lacking. this study explored the influence of k deficiency on c-n interaction in cotton leaves by conducting a field experiment with cotton cultivar dp0912 under two k rates (k0: 0 kg k2o ha-1 and k67: 67 kg k2o ha-1) and a controlled environment experiment with k-deficient solution (k1: 0 mm k+) and k-sufficient solution (k2: 6 mm k+). the results showed th ...201728441628
abp9, a maize bzip transcription factor, enhances tolerance to salt and drought in transgenic cotton.abp9 , encoding a bzip transcription factor from maize, enhances tolerance to multiple stresses and may participate in the aba signaling pathway in transgenic cotton by altering physiological and biochemical processes and stress-related gene expression. abiotic stresses, such as soil salinity and drought, negatively affect growth, development, and yield in cotton. gene abp9, which encodes a bzip transcription factor, binds to the abscisic acid (aba)-responsive-element (abre2) motif of the maize ...201728474114
within-plant distribution and dynamics of thrips species (thysanoptera: thripidae) in cotton.a 2-yr study in cotton (gossypium hirsutum l.) was conducted to determine the abundance and species composition of thrips (thysanoptera: thripidae) on different plant parts throughout the season in alabama, georgia, north carolina, south carolina, and virginia. plant parts sampled included seedlings, terminals with two expanded leaves, leaves from the upper, middle, and lower sections of the canopy, white flowers, and medium-sized bolls. adult thrips were significantly more abundant on seedlings ...201728475718
comparative genomic study of aldh gene superfamily in gossypium: a focus on gossypium hirsutum under salt stress.aldehyde dehydrogenases (aldhs) are a superfamily of enzymes which play important role in the scavenging of active aldehydes molecules. in present work, a comprehensive whole-genomic study of aldh gene superfamily was carried out for an allotetraploid cultivated cotton species, g. hirsutum, as well as in parallel relative to their diploid progenitors, g. arboreum and g. raimondii. totally, 30 and 58 aldh gene sequences belong to 10 families were identified from diploid and allotetraploid cotton ...201728489898
high efficient multisites genome editing in allotetraploid cotton (gossypium hirsutum) using crispr/cas9 system.gossypium hirsutum is an allotetraploid with a complex genome. most genes have multiple copies that belong to at and dt subgenomes. sequence similarity is also very high between gene homologues. to efficiently achieve site/gene-specific mutation is quite needed. due to its high efficiency and robustness, the crispr (clustered regularly interspaced short palindromic repeats)/cas9 system has exerted broad site-specific genome editing from prokaryotes to eukaryotes. in this study, we utilized a cri ...201828499063
genome-wide characterization and expression analyses of superoxide dismutase (sod) genes in gossypium hirsutum.superoxide dismutases (sods) are a key antioxidant enzyme family, which have been implicated in protecting plants against the toxic effects of reactive oxygen species. despite current studies have shown that the gene family are involved in plant growth and developmental processes and biotic and abiotic stress responses, little is known about its functional role in upland cotton.201728499417
field trial of insect-resistant and herbicide-tolerant genetically modified cotton (gossypium hirsutum l.) for environmental risk assessment in japan.japan imports cottonseed mainly from australia and the usa where more than 96% of all cotton varieties grown are genetically modified (gm). gm crops undergo an environmental risk assessment (era) under the law concerning the conservation and sustainable use of biological diversity before import into japan. potential adverse effects on biodiversity are comprehensively assessed based on competitiveness, production of harmful substances and outcrossing ability. even though imported cottonseed is in ...201728510512
comparison of rhizosphere properties as affected by different bt- and non-bt-cotton (gossypium hirsutum l.) genotypes and fertilization.incorporation of genetically modified crops in the cropping system raises the need for studying the effect of these crops on the soil ecosystem. the current study aimed to compare the effect of bacillus thuringiensis (bt)- and non-bt-cotton (gossypium hirsutum l.) genotypes on rhizosphere properties under fertilized and unfertilized soil conditions. one non-bt-cotton (iub 75) and four bt-cotton varieties (iub-222, mm-58, iub-13, fh-142) were sown in a randomized complete block design (rcbd) in a ...201728527035
title: potassium application regulates nitrogen metabolism and osmotic adjustment in cotton (gossypium hirsutum l.) functional leaf under drought stress.to evaluate the role of potassium (k) in maintaining nitrogen metabolism and osmotic adjustment development of cotton functional leaves to sustain growth under soil drought and rewatering conditions, the plants of two cotton cultivars siza 3 (low-k sensitive) and simian 3 (low-k tolerant), were grown under three different k rates (k0, k1, and k2; 0, 150, and 300kgk2oha(-1), respectively) and exposed to drought stress with 40±5% soil relative water content (srwc). the drought stress was applied a ...028527336
cotton gherf38 gene is involved in plant response to salt/drought and aba.erf (ethylene-responsive factor) transcription factors play important roles in plant stress signaling transduction pathways. however, their specific roles during diverse abiotic stresses tolerance in gossypium hirsutum are largely unknown. here, a novel erf transcription factor, designated gherf38, homologous to aterf38 in arabidopsis, was isolated from cotton (gossypium hirsutum l). gherf38 expression was up-regulated by salt, drought and aba treatments. subcellular localization results indicat ...201728536792
detoxification of fusaric acid by the soil microbe mucor rouxii.fusarium oxysporum f. sp. vasinfectum race 4 (vcg0114), which causes root rot and wilt of cotton (gossypium hirsutum and g. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the san joaquin valley of california. this pathotype produces copious quantities of the plant toxin fusaric acid (5-butyl-2-pyridinecarboxylic acid) compared to other isolates of f. oxysporum f. sp. vasinfectum (fov) that are indigenous to the united states. fusaric a ...201728538103
role of xyloglucan in cotton (gossypium hirsutum l.) fiber elongation of the short fiber mutant ligon lintless-2 (li2).xyloglucan is a matrix polysaccharide found in the cell walls of all land plants. in growing cells, xyloglucan is thought to connect cellulose microfibrils and regulate their separation during wall extension. ligon lintless-2 (li2) is a monogenic dominant cotton fiber mutation that causes extreme reduction in lint fiber length with no pleiotropic effects on vegetative growth. li2 represents an excellent model system to study fiber elongation. to understand the role of xyloglucan in cotton fiber ...201728546126
assessment of gene flow between gossypium hirsutum and g. herbaceum: evidence of unreduced gametes in the diploid progenitor.in the framework of a gene flow assessment, we investigated the natural hybridization rate between gossypium hirsutum (aadd genome) and g. herbaceum (aa genome). the latter species, a diploid progenitor of g. hirsutum, is spontaneously present in south africa. reciprocal crosses were performed without emasculation between g. herbaceum and g. hirsutum neither examination of the morphological characteristics nor flow cytometry analysis of the 335 plants resulting from the g. hirsutum × g. herbaceu ...201728546386
genome-wide identification and characterization of phospholipase c gene family in cotton (gossypium spp.).phospholipase c (plc) are important regulatory enzymes involved in several lipid and ca2+-dependent signaling pathways. previous studies have elucidated the versatile roles of plc genes in growth, development and stress responses of many plants, however, the systematic analyses of plc genes in the important fiber-producing plant, cotton, are still deficient. in this study, through genome-wide survey, we identified twelve phosphatidylinositol-specific plc (pi-plc) and nine non-specific plc (npc) ...201728547583
tobacco rattle virus-based silencing of enoyl-coa reductase gene and its role in resistance against cotton wilt disease.a tobacco rattle virus (trv)-based virus-induced gene silencing assay was employed as a reverse genetic approach to study gene function in cotton (gossypium hirsutum). this approach was used to investigate the function of the enoyl-coa reductase (ghecr) gene in pathogen defense. amino acid sequence alignment of arabidopsis ecr with homologous sequence from g. hirsutum, g. arboreum, g. herbaceum and g. barbadense showed that ecrs are highly conserved among these species. trv-based silencing of gh ...201728567686
identification of candidate genes for fiber length quantitative trait loci through rna-seq and linkage and physical mapping in cotton.cotton (gossypium spp.) fibers are single-celled elongated trichomes, the molecular aspects of genetic variation in fiber length (fl) among genotypes are currently unknown. in this study, two backcross inbred lines (bils), i.e., nmga-062 ("long") and nmga-105 ("short") with 32.1 vs. 27.2 mm in fl, respectively, were chosen to perform rna-seq on developing fibers at 10 days post anthesis (dpa). the two bils differed in 4 quantitative trait loci (qtl) for fl and were developed from backcrosses bet ...201728569138
genome-wide analysis of gene expression of ems-induced short fiber mutant ligon lintless-y (liy) in cotton (gossypium hirsutum l.).in this work we describe a chemically-induced short fiber mutant cotton line, ligon-lintless-y (liy), which is controlled by a single recessive locus and affects multiple traits, including height of the plant, and length and maturity of fiber. an rnaseq analysis was used to evaluate global transcriptional changes during cotton fiber development at 3, 8 and 16days post anthesis. we found that 613, 2629 and 3397 genes were significantly down-regulated, while 2700, 477 and 3260 were significantly u ...201728577792
carbohydrate metabolism in the subtending leaf cross-acclimates to waterlogging and elevated temperature stress and influences boll biomass in cotton (gossypium hirsutum).short-term waterlogging and chronic elevated temperature occur concomitantly in the cotton (gossypium hirsutum) growing season. while previous research about co-occurring waterlogging and elevated temperature has focused primarily on cotton fiber, no studies have investigated carbohydrate metabolism of the subtending leaf (a major source leaf for boll development) cross-acclimation to aforementioned stressors. to address this, plants were exposed to ambient (31.6/26.5°c) and elevated (34.1/29.0° ...201728581029
genomic analyses in cotton identify signatures of selection and loci associated with fiber quality and yield traits.upland cotton (gossypium hirsutum) is the most important natural fiber crop in the world. the overall genetic diversity among cultivated species of cotton and the genetic changes that occurred during their improvement are poorly understood. here we report a comprehensive genomic assessment of modern improved upland cotton based on the genome-wide resequencing of 318 landraces and modern improved cultivars or lines. we detected more associated loci for lint yield than for fiber quality, which sug ...201728581501
genome-wide identification and characterization of jaz gene family in upland cotton (gossypium hirsutum).plant jaz (jasmonate zim-domain) proteins play versatile roles in multiple aspects of plant development and defense. however, little is known about the jaz family in allotetraploid upland cotton (gossypium hirsutum) so far. in this study, 30 non-redundant jaz genes were identified in upland cotton through genome-wide screening. phylogenetic analysis revealed that the 30 proteins in cotton jaz family are further divided into five groups (i - v), and members in the same group share highly conserve ...201728584307
the specific host plant dna detection suggests a potential migration of apolygus lucorum from cotton to mungbean fields.the polyphagous mirid bug apolygus lucorum (heteroptera: miridae) has more than 200 species of host plants and is an insect pest of important agricultural crops, including cotton (gossypium hirsutum) and mungbean (vigna radiata). previous field trials have shown that a. lucorum adults prefer mungbean to cotton plants, indicating the considerable potential of mungbean as a trap crop in cotton fields. however, direct evidence supporting the migration of a. lucorum adults from cotton to mungbean is ...201728586352
identification and characterization of constans-like (col) gene family in upland cotton (gossypium hirsutum l.).the constans/flowering locus t (co/ft) regulon plays a central role in the control of flowering time in photoperiod-sensitive plants. flowering time in wild cotton (gossypium spp.) has strict photoperiod sensitivity, but domesticated cotton is day-neutral. information on the molecular characterization of the co and co-like (col) genes in cotton is very limited. in this study, we identified 42 col homologs (ghcols) in the g. hirsutum genome, and many of them were previously unreported. we studied ...201728591177
a novel ghbee1-like gene of cotton causes anther indehiscence in transgenic arabidopsis under uncontrolled transcription level.male-sterile lines are very important for selective breeding, and anther dehiscence defect is an effective way to generate male-sterile lines. although several bhlh-family proteins in arabidopsis have been characterized, little is known about the role of bhlh-family proteins in cotton. here, we isolated a novel bhlh protein from cotton (gossypium hirsutum), named ghbee1-like. protein domain analysis showed that ghbee1-like contained a basic domain and an hlh domain. subcellular localization anal ...201728600178
a new species of scopulariopsis and its synergistic effect on pathogenicity of verticillium dahliae on cotton plants.a new species, scopulariopsis gossypii, was found to be present in the vascular bundles of cotton plants (gossypium hirsutum) infected by verticillium dahliae which is an economically important pathogen in hangzhou, china. the fungus was only present in the diseased plants, but it never became isolated from the healthy plants. scanning electron microscopy showed that the fungus was present in parenchyma cells enclosing vessels in dark brown vascular tissues of stems, and produced asexual conidia ...201728602397
expression and functional analyses of a kinesin gene ghkis13a1 from cotton (gossypium hirsutum) fiber.cotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. a large number of genes are believed to be involved in fiber formation, but so far only a few fiber genes have been isolated and functionally characterized in this developmental process. the kinesin13 subfamily was found to play key roles during cell division and cell elongation, and was considered to be involved in the regulation of cotton fiber development.201728606082
genome-wide identification and characterization of snrk2 gene family in cotton (gossypium hirsutum l.).sucrose non-fermenting-1-related protein kinase 2 (snrk2) is a plant-specific serine/threonine kinase family involved in the abscisic acid (aba) signaling pathway and responds to osmotic stress. a genome-wide analysis of this protein family has been conducted previously in some plant species, but little is known about snrk2 genes in upland cotton (gossypium hirsutum l.). the recent release of the g. hirsutum genome sequence provides an opportunity to identify and characterize the snrk2 kinase fa ...201728606097
genome-wide identification and characterization of micrornas differentially expressed in fibers in a cotton phytochrome a1 rnai line.cotton fiber is an important commodity throughout the world. fiber property determines fiber quality and commercial values. previous studies showed that silencing phytochrome a1 gene (phya1) by rna interference in upland cotton (gossypium hirsutum l. cv. coker 312) had generated phya1 rnai lines with simultaneous improvements in fiber quality (longer, stronger and finer fiber) and other key agronomic traits. characterization of the altered molecular processes in these rnai genotypes and its wild ...201728614407
evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (gossypium hirsutum l.) cv. svpr-2.an efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. as cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. different carbon sources such as fructose, glucose, sucrose and maltose were tested at various concentrations to control phenolic secretion in callus culture. among them, 3% maltose was ...201528626717
micrornas involved in auxin signalling modulate male sterility under high-temperature stress in cotton (gossypium hirsutum).male sterility caused by long-term high-temperature (ht) stress occurs widely in crops. micrornas (mirnas), a class of endogenous non-coding small rnas, play an important role in the plant response to various abiotic stresses. to dissect the working principle of mirnas in male sterility under ht stress in cotton, a total of 112 known mirnas, 270 novel mirnas and 347 target genes were identified from anthers of ht-insensitive (84021) and ht-sensitive (h05) cotton cultivars under normal-temperatur ...201728635129
the variation in the rhizosphere microbiome of cotton with soil type, genotype and developmental stage.plant roots and soil microorganisms interact with each other mainly in the rhizosphere. changes in the community structure of the rhizosphere microbiome are influenced by many factors. in this study, we determined the community structure of rhizosphere bacteria in cotton, and studied the variation of rhizosphere bacterial community structure in different soil types and developmental stages using tm-1, an upland cotton cultivar (gossypium hirsutum l.) and hai 7124, a sea island cotton cultivar (g ...201728638057
cotton ascorbate oxidase promotes cell growth in cultured tobacco bright yellow-2 cells through generation of apoplast oxidation.ascorbate oxidase (ao) plays an important role in cell growth through the modulation of reduction/oxidation (redox) control of the apoplast. here, a cotton (gossypium hirsutum) apoplastic ascorbate oxidase gene (ghao1) was obtained from fast elongating fiber tissues. ghao1 belongs to the multicopper oxidase (mco) family and includes a signal peptide and several transmembrane regions. analyses of quantitative real-time polymerase chain reaction (qrt-pcr) and enzyme activity showed that ghao1 was ...201728644407
simultaneous silencing of ghfad2-1 and ghfatb enhances the quality of cottonseed oil with high oleic acid.cottonseed oil has become an important source of edible oil due to its significant cost advantage. however, there is a growing concern over its fatty acid composition and nutritional value. in gossypium hirsutum, ghfad2-1 and ghfatb encoding the microsomal oleate desaturase and palmitoyl-acyl carrier protein thioesterase, respectively, play critical roles in regulating the proportions of saturated and polyunsaturated fatty acids in cottonseed lipids. in this study, rnai technology was used to si ...201728644971
ghvln4 is involved in cell elongation via regulation of actin organization.ghvln4 exhibited activity of cross-linking actin filaments into bundles. overexpression of ghvln4 increased the abundance of thick actin bundles and resulted in longer cell phenotypes. actin bundle is a dynamic, higher-order cytoskeleton structure that is essential for cell expansion. villin is one of the major proteins responsible for crosslinking actin filaments into bundles. however, this kind of actin binding protein has rarely been investigated in cotton. in the present work, a cotton villi ...201728647813
ghjaz2 attenuates cotton resistance to biotic stresses via inhibiting the transcriptional activity of ghbhlh171.plants evolve effective mechanisms to protect themselves against multiple stresses and employ jasmonates (ja) as vital defense signals to defend against pathogen infection. accumulation of ja induced by signals from biotic and abiotic stresses, results in degradation of jasmonate-zim-domain (jaz) proteins, and then de-repressed the jaz-repressed transcription factors (such as myc2) to activate defense responses and developmental processes. here, we characterized a jaz family protein, ghjaz2, fro ...201728665036
effect of boron deficiency on anatomical structure and chemical composition of petioles and photosynthesis of leaves in cotton (gossypium hirsutum l.).the main symptom of boron (b) deficiency in cotton is the formation of brown rings on leaf petioles. the objective of the present study was to determine the changes in the anatomical structure and chemical composition of petioles and photosynthesis of leaves in cotton under b deficiency. compared to the control, b deficiency treatment resulted in large increases in the number of petioles with brown rings per plant (160.0%) and the number of rings on the petiole per functional leaf (711.1%) in co ...201728667263
genome-wide analysis of salinity-stress induced dna methylation alterations in cotton (gossypium hirsutum l.) using the me-dip sequencing technology.cytosine dna methylation is a significant form of dna modification closely associated with gene expression in eukaryotes, fungi, animals, and plants. although the reference genomes of cotton (gossypium hirsutum l.) have been publically available, the salinity-stress-induced dna methylome alterations in cotton are not well understood. here, we constructed a map of genome-wide dna methylation characteristics of cotton leaves under salt stress using the methylated dna immunoprecipitation sequencing ...201728671256
the pin gene family in cotton (gossypium hirsutum): genome-wide identification and gene expression analyses during root development and abiotic stress responses.cell elongation and expansion are significant contributors to plant growth and morphogenesis, and are often regulated by environmental cues and endogenous hormones. auxin is one of the most important phytohormones involved in the regulation of plant growth and development and plays key roles in plant cell expansion and elongation. cotton fiber cells are a model system for studying cell elongation due to their large size. cotton is also the world's most utilized crop for the production of natural ...201728673242
proteomic analysis of differences in fiber development between wild and cultivated gossypium hirsutum l.upland cotton (gossypium hirsutum l.) is one of the world's most important fiber crops, accounting for more than 90% of all cotton production. while their wild progenitors have relatively short and coarse, often tan-colored fibers, modern cotton cultivars possess longer, finer, stronger, and whiter fiber. in this study, the wild and cultivated cottons (yu-3 and tm-1) selected show significant differences on fibers at 10 days postanthesis (dpa), 20 dpa, and mature stages at the morphological leve ...201728683551
genome-wide analysis of wox genes in upland cotton and their expression pattern under different stresses.wuschel-related homeobox (wox) family members play significant roles in plant growth and development, such as in embryo patterning, stem-cell maintenance, and lateral organ formation. the recently published cotton genome sequences allow us to perform comprehensive genome-wide analysis and characterization of wox genes in cotton.201728683794
evolution, gene expression profiling and 3d modeling of csld proteins in cotton.among cesa-like gene superfamily, the cellulose synthase-like d (csld) genes are most similar to cellulose synthase genes and have been reported to be involved in tip-growing cell and stem development. however, there has been no genome-wide characterization of this gene subfamily in cotton. we thus sought to analyze the evolution and functional characterization of csld proteins in cotton based on fully sequenced cotton genomes.201728693426
regulation of cotton (gossypium hirsutum) drought responses by mitogen-activated protein (map) kinase cascade-mediated phosphorylation of ghwrky59.drought is a key limiting factor for cotton (gossypium spp.) production, as more than half of the global cotton supply is grown in regions with high water shortage. however, the underlying mechanism of the response of cotton to drought stress remains elusive. by combining genome-wide transcriptome profiling and a loss-of-function screen using virus-induced gene silencing, we identified gossypium hirsutum ghwrky59 as an important transcription factor that regulates the drought stress response in ...201728700082
identification and functional analysis of two p450 enzymes of gossypium hirsutum involved in dmnt and tmtt biosynthesis.the homoterpenes (3e)-4,8-dimethyl-1,3,7-nonatriene (dmnt) and (e,e)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (tmtt) are major herbivore-induced plant volatiles that can attract predatory or parasitic arthropods to protect injured plants from herbivore attack. in this study, dmnt and tmtt were confirmed to be emitted from cotton (gossypium hirsutum) plants infested with chewing caterpillars or sucking bugs. two cyp genes (ghcyp82l1 and ghcyp82l2) involved in homoterpene biosynthesis in g. hirsu ...201728710782
global analysis of dna methylation in young (j1) and senescent (j2) gossypium hirsutum l. cotyledons by medip-seq.dna methylation is an important epigenetic modification regulating gene expression, genomic imprinting, transposon silencing and chromatin structure in plants and plays an important role in leaf senescence. however, the dna methylation pattern during gossypium hirsutum l. cotyledon senescence is poorly understood. in this study, global dna methylation patterns were compared between two cotyledon development stages, young (j1) and senescence (j2), using methylated dna immunoprecipitation (medip-s ...201728715427
tissue and cell-specific transcriptomes in cotton reveal the subtleties of gene regulation underlying the diversity of plant secondary cell walls.knowledge of plant secondary cell wall (scw) regulation and deposition is mainly based on the arabidopsis model of a 'typical' lignocellulosic scw. however, scws in other plants can vary from this. the scw of mature cotton seed fibres is highly cellulosic and lacks lignification whereas xylem scws are lignocellulosic. we used cotton as a model to study different scws and the expression of the genes involved in their formation via rna deep sequencing and chemical analysis of stem and seed fibre.201728720072
ghsos1, a plasma membrane na+/h+ antiporter gene from upland cotton, enhances salt tolerance in transgenic arabidopsis thaliana.upland cotton (gossypium hirsutum l.), an important source of natural fiber, can tolerate relatively high salinity and drought stresses. in the present study, a plasma membrane na+/h+ antiporter gene, ghsos1, was cloned from a salt-tolerant genotype of g. hirsutum, zhong 9807. the expression level of ghsos1 in cotton roots was significantly upregulated in the presence of high concentrations of nacl (200 mm), while its transcript abundance was increased when exposed to low temperature and drought ...201728723926
enriching an intraspecific genetic map and identifying qtl for fiber quality and yield component traits across multiple environments in upland cotton (gossypium hirsutum l.).cotton is a significant commercial crop that plays an indispensable role in many domains. constructing high-density genetic maps and identifying stable quantitative trait locus (qtl) controlling agronomic traits are necessary prerequisites for marker-assisted selection (mas). a total of 14,899 ssr primer pairs designed from the genome sequence of g. raimondii were screened for polymorphic markers between mapping parents ccri 35 and yumian 1, and 712 ssr markers showing polymorphism were used to ...201728733817
suppressing a putative sterol carrier gene reduces plasmodesmal permeability and activates sucrose transporter genes during cotton fiber elongation.plasmodesmata (pds) play vital roles in cell-to-cell communication and plant development. emerging evidence suggests that sterols are involved in pd activity during cytokinesis. however, whether sterols contribute to pd gating between established cells remains unknown. here, we isolated ghscp2d, a putative sterol carrier protein gene from elongating cotton (gossypium hirsutum) fibers. in contrast to wild-type fiber pds, which opened at 5 to 10 d postanthesis (dpa) and closed only at 15 to 25 dpa ...201728747422
association mapping analysis of fiber yield and quality traits in upland cotton (gossypium hirsutum l.).fiber yield and quality are the most important traits for upland cotton (gossypium hirsutum l.). identifying high yield and good fiber quality genes are the prime concern of researchers in cotton breeding. association mapping offers an alternative and powerful method for detecting those complex agronomic traits. in this study, 198 simple sequence repeats (ssrs) were used to screen markers associated with fiber yield and quality traits with 302 elite upland cotton accessions that were evaluated i ...201728748394
5-aminolevulinic acid dehydratase gene dosage affects programmed cell death and immunity.programmed cell death (pcd) is an important form to protect plants from pathogen attack. however, plants must precisely control the pcd process under microbe attacks to avoid detrimental effects. the complexity of how plants balance the defense activation and pcd requires further clarification. lesion mimic mutants constitute an excellent material to study the crosstalk between them. here, we identified a gossypium hirsutum (cotton) lesion mimic mutant (ghlmm), which exhibits necrotic leaf damag ...201728751313
genetic diversity and structure of elite cotton germplasm (gossypium hirsutum l.) using genome-wide snp data.cotton (gossypium spp.) is the most important natural textile fiber crop, and gossypium hirsutum l. is responsible for 90% of the annual cotton crop in the world. information on cotton genetic diversity and population structure is essential for new breeding lines. in this study, we analyzed population structure and genetic diversity of 288 elite gossypium hirsutum cultivar accessions collected from around the world, and especially from china, using genome-wide single nucleotide polymorphisms (sn ...201728755130
expression of pathogenesis-related genes in cotton roots in response to verticillium dahliae pamp molecules.verticillium wilt disease becomes a major threat to many economically important crops. it is unclear whether and how plant immunity takes place during cotton-verticillium interaction due to the lack of marker genes. taking advantage of cotton (gossypium hirsutum) genome, we discovered pathogenesis-related (pr) gene families, which have been widely used as markers of immune responses in plants. to profile the expression of g. hirsutum pr genes in the process of plant immunity, we treated cotton r ...201728755291
large-scale identification of gossypium hirsutum genes associated with verticillium dahliae by comparative transcriptomic and reverse genetics analysis.verticillium wilt is a devastating disease of cotton, which is caused by the soil-borne fungus verticillium dahliae (v. dahliae). although previous studies have identified some genes or biological processes involved in the interaction between cotton and v. dahliae, its underlying molecular mechanism remains unclear, especially in g. hirsutum. in the present study, we obtained an overview of transcriptome characteristics of resistant upland cotton (g. hirsutum) after v. dahliae infection at 24 h ...201728767675
the genome of the cotton bacterial blight pathogen xanthomonas citri pv. malvacearum strain msct1.xanthomonas citri pv. malvacearum is a major pathogen of cotton, gossypium hirsutum l.. in this study we report the complete genome of the x. citri pv. malvacearum strain msct1 assembled from long read dna sequencing technology. the msct1 genome is the first x. citri pv. malvacearum genome with complete coding regions for x. citri pv. malvacearum transcriptional activator-like effectors. in addition functional and structural annotations are presented in this study that will provide a foundation ...201728770027
a high-throughput standard pcr-based genotyping method for determining transgene zygosity in segregating plant populations.in crop research programs that implement transgene-based strategies for trait improvement it is necessary to distinguish between transgene homozygous and hemizygous individuals in segregating populations. direct methods for determining transgene zygosity are technically challenging, expensive, and require specialized equipment. in this report, we describe a standard pcr-based protocol coupled with capillary electrophoresis that can identify transgene homozygous and hemizygous individuals in a se ...201728791034
transfer of tomato immune receptor ve1 confers ave1-dependent verticillium resistance in tobacco and cotton.verticillium wilts caused by soilborne fungal species of the verticillium genus are economically important plant diseases that affect a wide range of host plants and are notoriously difficult to combat. perception of pathogen(-induced) ligands by plant immune receptors is a key component of plant innate immunity. in tomato, race-specific resistance to verticillium wilt is governed by the cell surface-localized immune receptor ve1 through recognition of the effector protein ave1 that is secreted ...201728796297
segregation distortion and genome-wide digenic interactions affect transmission of introgressed chromatin from wild cotton species.this study reports transmission genetics of chromosomal segments into gossypium hirsutum from its most distant euploid relative, gossypium mustelinum . mutilocus interactions and structural rearrangements affect introgression and segregation of donor chromatin. wild allotetraploid relatives of cotton are a rich source of genetic diversity that can be used in genetic improvement, but linkage drag and non-mendelian transmission genetics are prevalent in interspecific crosses. these problems necess ...201728801756
high-density genetic linkage map construction by f2 populations and qtl analysis of early-maturity traits in upland cotton (gossypium hirsutum l.).due to china's rapidly increasing population, the total arable land area has dramatically decreased; as a consequence, the competition for farming land allocated for grain and cotton production has become fierce. therefore, to overcome the existing contradiction between cotton grain and fiber production and the limited farming land, development of early-maturing cultivars is necessary. in this research, a high-density linkage map of upland cotton was constructed using genotyping by sequencing (g ...201728809947
genome editing in cotton with the crispr/cas9 system.genome editing is an important tool for gene functional studies as well as crop improvement. the recent development of the crispr/cas9 system using single guide rna molecules (sgrnas) to direct precise double strand breaks in the genome has the potential to revolutionize agriculture. unfortunately, not all sgrnas are equally efficient and it is difficult to predict their efficiency by bioinformatics. in crops such as cotton (gossypium hirsutum l.), with labor-intensive and lengthy transformation ...201728824692
genome-wide identification of cbl family and expression analysis of cbls in response to potassium deficiency in cotton.calcineurin b-like (cbl) proteins, as calcium sensors, play pivotal roles in plant responses to diverse abiotic stresses and in growth and development through interaction with cbl-interacting protein kinases (cipks). however, knowledge about functions and evolution of cbls in gossypium plants is scarce. here, we conducted a genome-wide survey and identified 13, 13 and 22 cbl genes in the progenitor diploid gossypium arboreum and gossypium raimondii, and the cultivated allotetraploid gossypium hi ...201728828254
flavobacterium gossypii sp. nov. isolated from the root tissue of field-grown cotton.a yellow-pigmented bacterial strain, jm-222t, isolated from the root tissue of field-grown cotton (gossypium hirsutum) in auburn, alabama, usa, was studied for its taxonomic allocation. cells of the isolate were rod-shaped and gram-stain-negative. a blast analysis using the eztaxon database showed that strain jm-222t shared highest 16s rrna gene sequence similarity with the type strain of flavobacterium endophyticum (98.9 %) followed by flavobacterium qiangtangense (96.7 %). 16s rrna gene sequen ...201728840797
potassium improves photosynthetic tolerance to and recovery from episodic drought stress in functional leaves of cotton (gossypium hirsutum l.).to investigate whether potassium (k) application enhances the potential of cotton (gossypium hirsutum l.) plants to maintain physiological functions during drought and recovery, low k-sensitive (siza 3) and -tolerant (simian 3) cotton cultivars were exposed to three k rates (0, 150, and 300 k2o kg ha-1) and either well-watered conditions or severe drought stress followed by a recovery period. under drought stress, cotton plants showed a substantial decline in leaf water potential, stomatal condu ...201728843133
genome-wide identification and characterization of tcp transcription factor genes in upland cotton (gossypium hirsutum).tcp proteins are plant-specific transcription factors (tfs), and perform a variety of physiological functions in plant growth and development. in this study, 74 non-redundant tcp genes were identified in upland cotton (gossypium hirsutum l.) genome. cotton tcp family can be classified into two classes (class i and class ii) that can be further divided into 11 types (groups) based on their motif composition. quantitative rt-pcr analysis indicated that ghtcps display different expression patterns ...201728860559
azotobacter chroococcum as a potentially useful bacterial biofertilizer for cotton (gossypium hirsutum): effect in reducing n fertilization.the aim of this research was to evaluate whether the application of two plant growth-promoting (rhizo)bacteria might reduce nitrogen fertilization doses in cotton. we used strains azotobacter chroococcum ac1 and ac10 for their proven ability to promote seed germination and cotton growth. these microorganisms were characterized by their plant growth-promoting activities. then, we conducted a glasshouse study to evaluate the plant growth promoting ability of these strains with reduced doses of ure ...201728864227
relative contribution of na+/k+ homeostasis, photochemical efficiency and antioxidant defense system to differential salt tolerance in cotton (gossypium hirsutum l.) cultivars.in this study, the role of specific components of different coping strategies to salt load were identified. a pot experiment was conducted with four cotton (gossypium hirsutum l.) cultivars (differing in salt-sensitivity) under salinity stress. based on observed responses in growth performance and physiological characteristics, cz91 was the most tolerant of the four cultivars, followed by cultivars ccri44 and ccri49, with z571 being much more sensitive to salt stress. to perform this tolerant re ...201728866234
investigation of the influence of leaf thickness on canopy reflectance and physiological traits in upland and pima cotton populations.many systems for field-based, high-throughput phenotyping (fb-htp) quantify and characterize the reflected radiation from the crop canopy to derive phenotypes, as well as infer plant function and health status. however, given the technology's nascent status, it remains unknown how biophysical and physiological properties of the plant canopy impact downstream interpretation and application of canopy reflectance data. in that light, we assessed relationships between leaf thickness and several cano ...201728868055
transcriptome analysis suggests that chromosome introgression fragments from sea island cotton (gossypium barbadense) increase fiber strength in upland cotton (gossypium hirsutum).as high-strength cotton fibers are critical components of high quality cotton, developing cotton cultivars with high-strength fibers as well as high yield is a top priority for cotton development. recently, chromosome segment substitution lines (cssls) have been developed from high-yield upland cotton (gossypium hirsutum) crossed with high-quality sea island cotton (g. barbadense). here, we constructed a cssl population by crossing ccri45, a high-yield upland cotton cultivar, with hai1, a sea is ...201728874383
activation of aba receptors gene ghpyl9-11a is positively correlated with cotton drought tolerance in transgenic arabidopsis.the sensitivity to abscisic acid (aba) by its receptors, pyrabactin resistance-like proteins (pyls), is considered a most important factor in activating the aba signal pathway in response to abiotic stress. however, it is still unknown which pyl is the crucial aba receptor mediating response to drought stress in cotton (gossypium hirsutum l.). here, we reported the identification and characterization of highly induced aba receptor ghpyl9-11a in response to drought in cotton. it is observed that ...201728878793
comparative transcriptome analysis of cotton fiber development of upland cotton (gossypium hirsutum) and chromosome segment substitution lines from g. hirsutum × g. barbadense.how to develop new cotton varieties possessing high yield traits of upland cotton and superior fiber quality traits of sea island cotton remains a key task for cotton breeders and researchers. while multiple attempts bring in little significant progresses, the development of chromosome segment substitution lines (cssls) from gossypium barbadense in g. hirsutum background provided ideal materials for aforementioned breeding purposes in upland cotton improvement. based on the excellent fiber perfo ...201728886694
overexpression of ghdof1 improved salt and cold tolerance and seed oil content in gossypium hirsutum.a homologous ghdof1, which belongs to a large family of plant-specific transcription factor dof, was isolated from upland cotton (gossypium hirsutum l.). ghdof1 protein was located in the nucleus of onion epidermal cells, the core domain of transcriptional activity existed in the c-terminal, and the activity elements of ghdof1 promoter existed in the regions of -645∼ -469bp and -286∼ -132bp of transcriptional start codon. ghdof1 constitutively expressed in leaves, roots and stems, accumulated hi ...201728888163
genome-wide analysis of the gst gene family in gossypium hirsutum l.glutathione-s-transferase (gst) is a ubiquitous multi-functional protein superfamily that plays important roles in plant primary and secondary metabolism, stress and intercellular signal transduction. concomitantly, it also functions as a ligand in the metabolism of plant hormones and substance transport. in order to understand the gst gene family in upland cotton (gossypium hirsutum l.), herein we analyzed the species, evolutionary relationship, physical location, gene structure, conserved moti ...201728903901
genomics-enabled analysis of the emergent disease cotton bacterial blight.cotton bacterial blight (cbb), an important disease of (gossypium hirsutum) in the early 20th century, had been controlled by resistant germplasm for over half a century. recently, cbb re-emerged as an agronomic problem in the united states. here, we report analysis of cotton variety planting statistics that indicate a steady increase in the percentage of susceptible cotton varieties grown each year since 2009. phylogenetic analysis revealed that strains from the current outbreak cluster with ra ...201728910288
water quality of surface runoff and lint yield in cotton under furrow irrigation in northeast arkansas.use of furrow irrigation in row crop production is a common practice through much of the midsouth us and yet, nutrients can be transported off-site through surface runoff. a field study with cotton (gossypium hirsutum, l.) was conducted to understand the impact of furrow tillage practices and nitrogen (n) fertilizer placement on characteristics of runoff water quality during the growing season. the experiment was designed as a randomized complete block design with conventional (ct) and conservat ...201828910718
biological fabrication of cellulose fibers with tailored properties.cotton is a promising basis for wearable smart textiles. current approaches that rely on fiber coatings suffer from function loss during wear. we present an approach that allows biological incorporation of exogenous molecules into cotton fibers to tailor the material's functionality. in vitro model cultures of upland cotton (gossypium hirsutum) are incubated with 6-carboxyfluorescein-glucose and dysprosium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid-glucose, where the glucose moiety ...201728912238
structure of cellulose microfibrils in mature cotton fibres.the structure of cellulose microfibrils in mature cotton fibres from three varieties - gossypium hirsutum, g. barbadense and g. arboreum - has been investigated by a multi-technique approach combining small angle scattering techniques with spectroscopy and diffraction. cellulose microfibrils present a iβ-rich crystalline structure with limited surface disorder. small angle scattering (saxs and sans) data have been successfully fitted using a core-shell model and the results obtained indicate tha ...201728917888
cytogenetic maps of homoeologous chromosomes a h01 and d h01 and their integration with the genome assembly in gossypium hirsutum.cytogenetic maps of gossypium hirsutum (linnaeus, 1753) homoeologous chromosomes ah01 and dh01 were constructed by fluorescence in situ hybridization (fish), using eleven homoeologous-chromosomes-shared bacterial artificial chromosomes (bacs) clones and one chromosome-specific bac clone respectively. we compared the cytogenetic maps with the genetic linkage and draft genome assembly maps based on a standardized map unit, relative map position (rmp), which allowed a global view of the relationshi ...201728919972
itraq-based quantitative proteomic analysis reveals cold responsive proteins involved in leaf senescence in upland cotton (gossypium hirsutum l.).premature leaf senescence occurs in the ultimate phase of the plant, and it occurs through a complex series of actions regulated by stress, hormones and genes. in this study, a proteomic analysis was performed to analyze the factors that could induce premature leaf senescence in two cotton cultivars. we successfully identified 443 differential abundant proteins (daps) from 7388 high-confidence proteins at four stages between non-premature senescence (ns) and premature senescence (ps), among whic ...201728926933
genetic and phenotypic effects of chromosome segments introgressed from gossypium barbadense into gossypium hirsutum.mbi9915 is an introgression cotton line with excellent fiber quality. it was obtained by advanced backcrossing and continuous inbreeding from an interspecific cross between the upland cotton (gossypium hirsutum) cultivar ccri36 as the recurrent parent and the sea island cotton (g. barbadense) cultivar hai1, as the donor parent. to study the genetic effects of the introgressed chromosome segments in g. hirsutum, an f2 secondary segregating population of 1537 individuals was created by crossing mb ...201728931074
transcriptomic repertoires depict the initiation of lint and fuzz fibres in cotton (gossypium hirsutum l.).cotton fibre is an important natural fibre for the textile industry. the number of fibre initials determines the lint percentage, which is an important factor for cotton fibre yield. although fibre development has been described by transcriptomic analysis, the mechanism by which the long noncoding rna manipulates the initiation of lint and fuzz fibres remains unknown. in this study, three lines with different lint percentages were developed by crossing xu142 with its fibreless mutant xu142 fl. w ...201728944536
flavonoid profile of the cotton plant, gossypium hirsutum: a review.cotton, gossypium hirsutum l., is a plant fibre of significant economic importance, with seeds providing an additional source of protein in human and animal nutrition. flavonoids play a vital role in maintaining plant health and function and much research has investigated the role of flavonoids in plant defence and plant vigour and the influence these have on cotton production. as part of ongoing research into host plant/invertebrate pest interactions, we investigated the flavonoid profile of co ...201728946657
global gene expression in cotton (gossypium hirsutum l.) leaves to waterlogging stress.cotton is sensitive to waterlogging stress, which usually results in stunted growth and yield loss. to date, the molecular mechanisms underlying the responses to waterlogging in cotton remain elusive. cotton was grown in a rain-shelter and subjected to 0 (control)-, 10-, 15- and 20-d waterlogging at flowering stage. the fourth-leaves on the main-stem from the top were sampled and immediately frozen in liquid nitrogen for physiological measurement. global gene transcription in the leaves of 15-d ...201728953908
phospholipases dα and δ are involved in local and systemic wound responses of cotton (g. hirsutum).phospholipases d (plds) catabolize structural phospholipids to produce phosphatidic acid (ptdoh), a lipid playing central role in signalling pathways in animal, yeast and plant cells. in animal cells two pld genes have been studied while in model plant arabidopsis twelve genes exist, classified in six classes (α-ζ). this underlines the role of these enzymes in plant responses to environmental stresses. however, information concerning the pld involvement in the widely cultivated and economically ...201728955998
high concentrations of chlorantraniliprole reduce its compatibility with a key predator, hippodamia convergens (coleoptera: coccinellidae).diamides are a novel insecticide group that act by disrupting insect muscle contraction. recommended field rates (frs) vary greatly among target pests and cropping systems, leading to variable risks for non-target organisms. we evaluated the toxicity of chlorantraniliprole to the predator hippodamia convergens guérin-méneville (coleoptera: coccinellidae) by exposure to residues, topical application, and consumption of contaminated food. we also estimated lethal concentrations (lcs) of chlorantra ...201728961909
genome wide identification of cotton (gossypium hirsutum)-encoded microrna targets against cotton leaf curl burewala virus.cotton leaf curl burewala virus (clcubv, genus begomovirus) causes devastating cotton leaf curl disease. among various known virus controlling strategies, rnai-mediated one has shown potential to protect host crop plants. micro(mi) rnas, are the endogenous small rnas and play a key role in plant development and stress resistance. in the present study we have identified cotton (gossypium hirsutum)-encoded mirnas targeting the clcubv. based on threshold free energy and maximum complementarity scor ...201828964896
characterization of conserved circular rna in polyploid gossypium species and their ancestors.circular rna (circrna) is a regulatory class of long, noncoding rna found in both plant and animal kingdoms. the profile and characterization of circrna in cotton species remains to be explored. here, using 24 rrna-depleted rna-seq libraries of putative diploid progenitors of gossypium spp., gossypium arboreum and gossypium raimondii, their interspecies hybrid (f1 ) and allotetraploid gossypium hirsutum, 1041, 1478, 1311, and 499 circrnas were identified in each cotton species, respectively. a p ...201728971489
genetic diversity of arboreal cotton populations of the brazilian semiarid: a remnant primary gene pool for cotton cultivars.mocó cotton belong to the same species as the cultivated species, gossypium hirsutum, and cultivated forms were mainly landraces but also developed as cultivars, bearing good fiber quality and drought tolerant when cropped as a perennial species. the northeast brazil crop system based on this cotton type is finished, with a few small area planted in the three main states, where it was previously cultivated (ceará, paraíba, and rio grande do norte), but in others, maintenance is accomplished by s ...201728973721
molecular analysis of caffeoyl residues related to pigmentation in green cotton fibers.the pigment components in green cotton fibers were isolated and identified as 22-o-caffeoyl-22-hydroxymonodocosanoin and 22-o-caffeoyl-22-hydroxydocosanoic acid. the concentration of 22-o-caffeoyl-22-hydroxymonodocosanoin correlated positively with the degree of colour in the green fibers, indicating a role for caffeoyl derivatives in the pigmentation of green cotton fibers. upland cotton (gossypium hirsutum l.) contains four genes, gh4cl1-gh4cl4, encoding 4-coumarate:coa ligases (4cls), key enz ...201728981784
endogenous ethylene concentration is not a major determinant of fruit abscission in heat-stressed cotton (gossypium hirsutum l.).we investigated the role of ethylene in the response of cotton to high temperature using cotton genotypes with genetically interrupted ethylene metabolism. in the first experiment, sicot 71brf and 5b (a lintless variant with compromised ethylene metabolism) were exposed to 45°c, either by instantaneous heat shock or by ramping temperatures by 3°c daily for 1 week. one day prior to the start of heat treatment, half the plants were sprayed with 0.8 mm of the ethylene synthesis inhibitor, aminoetho ...201728983303
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