Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| crystal structure of class i acetohydroxy acid isomeroreductase from pseudomonas aeruginosa. | acetohydroxy acid isomeroreductase (ahir) is a key enzyme in the biosynthesis of branched-chain amino acids. we have determined the first crystal structure of a class i ahir from pseudomonas aeruginosa at 2.0 a resolution. its dodecameric architecture of 23 point group symmetry is assembled of six dimeric units and dimerization is essential for the formation of the active site. the dimeric unit of p.aeruginosa ahir partially superimposes with a three-domain monomer of spinach ahir, a class ii en ... | 2003 | 12691757 |
| effect of various temperatures on phosphatidylglycerol biosynthesis in thylakoid membranes. | lipid unsaturation, the major factor to maintain thylakoid membrane fluidity, is affected by temperature. in this work, we analysed the molecular species composition of phosphatidylglycerol (pg) in thylakoid membranes during spinach (spinacia oleracea) and squash (cucurbita pepo) cotyledon growth to investigate how the growth temperature affects the pg biosynthesis. of the 10 molecular species detected, temperature affected mainly the relative content of molecular species containing linolenic ac ... | 2003 | 12702014 |
| dual coenzyme specificity of photosynthetic glyceraldehyde-3-phosphate dehydrogenase interpreted by the crystal structure of a4 isoform complexed with nad. | photosynthetic glyceraldehyde-3-phosphate dehydrogenase (gapdh) of spinacia oleracea belongs to a wide group of gapdhs found in most organisms displaying oxygenic photosynthesis, including cyanobacteria, green and red algae, and higher plants. as a major catalytic difference with respect to glycolytic gapdh, photosynthetic gapdh exhibits dual cofactor specificity toward pyridine nucleotides with a preference for nadp(h). here we report the crystal structure of nad-complexed recombinant a(4)-gapd ... | 2003 | 12705826 |
| native uridine 5'-diphosphate-sulfoquinovose synthase, sqd1, from spinach purifies as a 250-kda complex. | sulfoquinovosyldiacylglycerol is a polar lipid present in photosynthetic membranes. it contributes to the negative surface charge of the membrane and plays a pivotal role under phosphate stress. the sqd1 protein is the key enzyme involved in the formation of the sulfolipid head group precursor, uridine 5(')-diphosphate (udp)-sulfoquinovose, from udp-glucose and sulfite. a cdna encoding the spinach sqd1 protein was isolated and functionally expressed in escherichia coli. the recombinant enzyme wa ... | 2003 | 12706349 |
| dynamic interaction between the d1 protein, cp43 and oec33 at the lumenal side of photosystem ii in spinach chloroplasts: evidence from light-induced cross-linking of the proteins in the donor-side photoinhibition. | during the donor-side photoinhibition of spinach photosystem ii, the reaction center d1 protein cross-linked with the antenna chlorophyll binding protein cp43 of photosystem ii lacking the oxygen-evolving complex (oec) subunit proteins. the cross-linking did not occur upon illumination of photosystem ii samples that retained the oec33, nor when oec33-depleted photosystem ii samples were reconstituted with the oec33 prior to illumination. these results suggest that the d1 protein, cp43 and the oe ... | 2003 | 12721387 |
| overexpression of the sucrose transporter sosut1 in potato results in alterations in leaf carbon partitioning and in tuber metabolism but has little impact on tuber morphology. | the aim of this work was to examine the consequences of the heterologous expression of a spinach ( spinacia oleracea l.) sucrose transporter ( sosut1) in potato ( solanum tuberosum l.). many studies have indicated that reduction of the expression of this class of sucrose transporter has deleterious effects on plant growth and development; however, until now the possibility of improving plant performance by enhancing the expression of this sucrose transporter has not been reported. with this inte ... | 2003 | 12721860 |
| overexpression and reconstitution of a rieske iron-sulfur protein from the higher plant. | the iron-sulfur protein subunit, known as the rieske protein, is one of the central components of the cytochrome b(6)f complex residing in chloroplast and cyanobacterial thylakoid membranes. we have constructed plasmids for overexpression in escherichia coli of full-length and truncated rieske (petc) proteins from the spinacia oleracea fused to male. overexpressed fusion proteins were predominantly found (from 55 to 70%) in cytoplasm in a soluble form. the single affinity chromatography step (am ... | 2003 | 12729720 |
| concentrations of choline-containing compounds and betaine in common foods. | choline is important for normal membrane function, acetylcholine synthesis and methyl group metabolism; the choline requirement for humans is 550 mg/d for men (adequate intake). betaine, a choline derivative, is important because of its role in the donation of methyl groups to homocysteine to form methionine. in tissues and foods, there are multiple choline compounds that contribute to total choline concentration (choline, glycerophosphocholine, phosphocholine, phosphatidylcholine and sphingomye ... | 2003 | 12730414 |
| processing of vegetable-borne carotenoids in the human stomach and duodenum. | carotenoids are thought to diminish the incidence of certain degenerative diseases, but the mechanisms involved in their intestinal absorption are poorly understood. our aim was to obtain basic data on the fate of carotenoids in the human stomach and duodenum. ten healthy men were intragastrically fed three liquid test meals differing only in the vegetable added 3 wk apart and in a random order. they contained 40 g sunflower oil and mashed vegetables as the sole source of carotenoids. tomato pur ... | 2003 | 12736146 |
| developmental co-variation of rna editing extent of plastid editing sites exhibiting similar cis-elements. | in tobacco, 30 of 34 sites in chloroplast transcripts that undergo c-to-u rna editing can be grouped into clusters of 2-5 sites based on sequence similarities immediately 5' to the edited c. according to a previous transgenic analysis, overexpression of transcripts representing one cluster member results in reduction in editing of all cluster members, suggesting that members of an individual cluster share a trans-factor that is present in limiting amounts. to compare leaves and roots, we quantif ... | 2003 | 12736308 |
| the anabolic pyruvate oxidoreductase from methanococcus maripaludis. | in autotrophic methanogens, pyruvate oxidoreductase (por) plays a key role in the assimilation of co(2) and the biosynthesis of organic carbon. this enzyme has been purified to homogeneity, and the genes from methanococcus maripaludis were sequenced. the purified por contained five polypeptides with molecular masses of 47, 33, 25, 21.5 and 13 kda. the n-terminal sequences of four of the polypeptides had high similarity to the subunits commonly associated with this enzyme from other archaea. howe ... | 2003 | 12743680 |
| reversed-phase hplc determination of chlorophyll a' and phylloquinone in photosystem i of oxygenic photosynthetic organisms. universal existence of one chlorophyll a' molecule in photosystem i. | chlorophyll (chl) a', the c132-epimer of chl a, is a constituent of the primary electron donor (p700) of photosystem (ps) i of a thermophilic cyanobacterium synechococcus (thermosynechococcus) elongatus, as was recently demonstrated by x-ray crystallography. to determine whether ps i of oxygenic photosynthetic organisms universally contains one molecule of chl a', pigment compositions of thylakoid membranes and ps i complexes isolated from the cyanobacteria t. elongatus and synechocystis sp. pcc ... | 2003 | 12755700 |
| actin-based photo-orientation movement of chloroplasts in plant cells. | in photosynthesizing plant cells, chloroplasts change their arrangements and/or positions in response to light irradiation. these photo-orientation movements of chloroplasts are believed to play important roles in optimizing the photosynthetic activity of plant cells. we have been investigating the roles of the actin cytoskeleton in the intracellular movement and positioning of chloroplasts using the aquatic monocot vallisneria gigantea graebner and the terrestrial dicot spinacia oleracea l. (sp ... | 2003 | 12756277 |
| delta opioid peptides derived from plant proteins. | opioid peptides showing selectivity for delta receptor have been isolated from enzymatic digests of plant proteins. five peptides were derived from wheat gluten, and named gluten exorphins a5, a4, b5, b4 and c. two opioid peptides were also released from spinach ribulose-bisphosphate-carboxylase/oxygenase (rubisco), and named rubiscolins-5 and -6. among them, gluten exorphin 5a (gly-tyr-tyr-pro-thr) and rubiscolin 6 (tyr-pro-leu-asp-leu-phe) improved learning performance in step-through type pas ... | 2003 | 12769740 |
| opioid receptor ligands derived from food proteins. | during the last two decades a variety of food protein fragments has been demonstrated to elicit biological effects in various in vitro or in vivo test systems. a considerable part of these bioactive peptides are opioid receptor ligands, which may be regarded as exogenous supplements to the endogenous opioidergic systems of the human organism. most of these food-derived opioid receptor ligands are fragments of the milk proteins alpha-, beta- or kappa-casein, alpha-lactalbumin, beta-lactoglobulin ... | 2003 | 12769741 |
| non-watson-crick basepairing and hydration in rna motifs: molecular dynamics of 5s rrna loop e. | explicit solvent and counterion molecular dynamics simulations have been carried out for a total of >80 ns on the bacterial and spinach chloroplast 5s rrna loop e motifs. the loop e sequences form unique duplex architectures composed of seven consecutive non-watson-crick basepairs. the starting structure of spinach chloroplast loop e was modeled using isostericity principles, and the simulations refined the geometries of the three non-watson-crick basepairs that differ from the consensus bacteri ... | 2003 | 12770867 |
| inhibition of clastogenicity of benzo[a]pyrene and of its trans-7,8-dihydrodiol in mice in vivo by fruits, vegetables, and flavonoids. | in the in vivo mouse bone marrow micronucleus assay, homogenates of spinach, artichoke, peaches, and blue grapes as well as commercial concentrates of these vegetables and fruits reduced induction of micronuclei by benzo[a]pyrene (bap) by 43-50%. concentrates of strawberries (31% reduction) and of cauliflower (20% reduction) were less potent. inhibition of genotoxicity by spinach and peaches was not caused by any delay in maturation of micronucleated erythrocytes as shown by experiments with sam ... | 2003 | 12787821 |
| sexual modification of female spinach seeds (spinacia oleracea l.) by irradiation with ion particles. | the female seeds of a spinach plant (spinacia orelacea l.) were exposed to he (12.5 mev/n) and c (18.3 mev/n) ions in order to investigate the effects of ion particles on sex expression. he ions did not affect germination rates or flowering at doses up to 50 gy. c ions did not affect germination rates or flowering at doses up to 15 gy, but a dose of 25 gy resulted in many plants with morphological aberrations. when unexposed female plants were grown without cross-fertilization for 10 weeks after ... | 2003 | 12789513 |
| assessment of essential and toxic elements in some kinds of vegetables. | vegetables provide the human body with the essential bioavailable trace elements, and a constant supply of these various elements is necessary and highly recommended for daily life. minor elements such as ca, fe, k, mg, and na as well as trace elements such as cd, co, cu, mn, ni, pb, and zn were determined using an atomic absorption spectrophotometer in 12 different species of vegetables from saudi arabia (the al-taif district). these vegetables were cucumber, vegetable marrow, tomato, potato, g ... | 2003 | 12798758 |
| evidence of cytochrome p450-catalyzed cleavage of the ether bond of phenoxybutyrate herbicides in rhodococcus erythropolis k2-3. | bacterial strain rhodococcus erythropolis k2-3 can cleave the ether bond of the phenoxybutyrate herbicides, i.e., 4-(2,4-dichlorophenoxy)butyrate (2,4-db) and 4-(4-chloro-2-methylphenoxy)butyrate (mcpb), by an enzyme system that is constitutively expressed. the enzyme(s) involved were investigated in this study. the rate of disappearance of 2,4-db determined in a whole cell assay amounted to 0.6 mmol/h x g(dry mass). carbon monoxide difference spectra of dithionite-reduced whole cells and crude ... | 2003 | 12801099 |
| spinach makes a safer anthrax vaccine. | 2003 | 12801787 | |
| the promoter of the spinach psaf gene for the subunit iii of the photosystem i reaction center directs beta-glucuronidase gene expression in transgenic tobacco roots. implication of the involvement of phospholipases and protein kinase c in psaf gene expression. | psaf is a nuclear encoded gene for the subunit iii of photosystem i. it is located at the lumenal side of the thylakoid membrane and interacts with plastocyanin. starting from a low-level expression in the cotyledons of etiolated seedlings the gene is upregulated by light. light can be replaced by ca2+ or phosphoinositides like phorbol myristate acetate, an analogue of diacylglycerol. we tested the effects of these components on psaf promoter-driven gene expression in roots and found that the ps ... | 2003 | 12806778 |
| bioavailability of soluble oxalate from spinach eaten with and without milk products. | leafy vegetables such as spinach (spinacia oleracea) are known to contain moderate amounts of soluble and insoluble oxalates. frozen commercially available spinach in new zealand contains 736.6+/-20.4 mg/100g wet matter (wm) soluble oxalate and 220.1+/-96.5mg/100g wm insoluble oxalate. the frozen spinach contained 90mg total calcium/100g wm, 76.7%of this calcium was unavailable as it was bound to oxalate as insoluble oxalate. the oxalate/calcium (meq) ratio of the frozen spinach was 4.73. when f ... | 2003 | 12810415 |
| field trials to assess the uptake of arsenic by vegetables from contaminated soils and soil remediation with iron oxides. | the uptake of arsenic (as) by plants from contaminated soils presents a health hazard that may affect the use of agricultural and former industrial land. methods for limiting the hazard are desirable. a proposed remediation treatment comprises the precipitation of iron (fe) oxides in the contaminated soil by adding ferrous sulfate and lime. the effects on as bioavailability were assessed using a range of vegetable crops grown in the field. four uk locations were used, where soil was contaminated ... | 2003 | 12826380 |
| proteomic characterization of the chlamydomonas reinhardtii chloroplast ribosome. identification of proteins unique to th e70 s ribosome. | we have conducted a proteomic analysis of the 70 s ribosome from the chlamydomonas reinhardtii chloroplast. twenty-seven orthologs of escherichia coli large subunit proteins were identified in the 50 s subunit, as well as an ortholog of the spinach plastid-specific ribosomal protein-6. several of the large subunit proteins of c. reinhardtii have short extension or insertion sequences, but overall the large subunit proteins are very similar to those of spinach chloroplast and e. coli. two protein ... | 2003 | 12826678 |
| metabolic cross talk between cytosolic and plastidial pathways of isoprenoid biosynthesis: unidirectional transport of intermediates across the chloroplast envelope membrane. | in higher plants, two independent pathways are responsible for the biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate, the central five-carbon precursors of all isoprenoids. the cytosolic pathway, which involves mevalonate (mva) as a key intermediate, provides the precursor molecules for sterols, ubiquinone, and certain sesquiterpenes, whereas the plastidial mva-independent pathway is involved in the formation of precursors for the biosynthesis of isoprene, monoterpenes, diter ... | 2003 | 12831836 |
| isolation and characterization of agar-degrading paenibacillus spp. associated with the rhizosphere of spinach. | agar-degrading bacteria in spinach plant roots cultivated in five soils were screened, and four strains of paenibacillus sp. were isolated from roots cultivated in three soils. the agar-degrading bacteria accounted for 1.3% to 2.5% of the total bacteria on the roots. in contrast, no agar-degrading colony was detected in any soil (non-rhizosphere soil samples) by the plate dilution method, and thus these agar-degrading bacteria may specifically inhabit plant roots. all isolates produced extracell ... | 2003 | 12834282 |
| protocol for the production of concentrated extracts of food folate for use in human bioavailability studies. | to provide a tool to study folate bioavailability under controlled conditions, a methodology was developed to produce extracts representative of natural food folates but removed from their matrix and sufficiently concentrated so as to elicit a response in biomarkers of folate status without distorting usual dietary intake patterns. egg, spinach, and yeast were selected to represent the wide range in extent of folate conjugation found in foods (0, 60, and 100% polyglutamyl folate, respectively). ... | 2003 | 12848514 |
| application of stable isotope dilution assays based on liquid chromatography-tandem mass spectrometry for the assessment of folate bioavailability. | a pilot study was performed to prove the suitability of stable isotope dilution assays for assessing the bioavailability of endogenous folates in foods. by using [2h(4)]folic acid, [2h(4)]tetrahydrofolate, [2h(4)]5-methyltetrahydrofolate, [2h(4)]5-formyltetrahydrofolate and [2h(4)]10-formylfolic acid as internal standards, folates in spinach, apple juice and blood plasma were quantified by liquid chromatography coupled to tandem mass spectrometry. to liberate the pteroyl monoglutamates, sample e ... | 2003 | 12860024 |
| structure-activity relationship of rubiscolins as delta opioid peptides. | to study the structure-activity relationship of rubiscolins (ypldlf and ypldl), delta opioid peptides derived from the spinach rubisco, we substituted the amino acid residues and evaluated their activities by mouse vas deferens (mvd) and guinea pig ileum (gpi) assays as well as receptor affinity. replacement of leu(3) with ile and met in rubiscolin-6 potentiated the delta opioid activity by about four times in mvd assay. asp(4) cannot be replaced by ala, glu or his. the original leu(5) was optim ... | 2003 | 12860192 |
| [comparative study of the biological utilization of beta-carotene from synthetic and natural sources in rats]. | the purpose of this study was to evaluate the change in concentration of retinol and beta-carotene (bc) in blood serum and liver tissue of rats, after supplementation with synthetic bc and commonly consumed carotenoid-rich vegetables (carrot and spinach). weanling male wistar rats were randomly assigned in four groups of 16 rats each. the four groups of rats were supplemented according to the following feeding treatments: 1) control group (0g), 0.2 ml corn oil; 2) pure bc group (bcg), 60 microgr ... | 2002 | 12868275 |
| functional differences of photosystem ii from synechococcus elongatus and spinach characterized by flash induced oxygen evolution patterns. | detailed comparative studies of flash induced oxygen evolution patterns in thylakoids from the thermophilic cyanobacterium synechococcus elongatus (s. elongatus; also referred to as thermosynechococcus elongatus) and from spinach led to the following results: (i) the miss parameter alpha of s. elongatus thylakoids exhibits a pronounced temperature dependence with a minimum of 7% at 25 degrees c and values of 17 and 10% at 3 and 35 degrees c, respectively, while for spinach thylakoids alpha decre ... | 2003 | 12885225 |
| two redox-active beta-carotene molecules in photosystem ii. | photosystem ii (ps ii) contains secondary electron-transfer paths involving cytochrome b(559) (cyt b(559)), chlorophyll (chl), and beta-carotene (car) that are active under conditions when oxygen evolution is blocked such as in inhibited samples or at low temperature. intermediates of the secondary electron-transfer pathways of ps ii core complexes from synechocystis pcc 6803 and synechococcus sp. and spinach ps ii membranes have been investigated using low temperature near-ir spectroscopy and e ... | 2003 | 12885246 |
| survey of nitrate and nitrite contents of vegetables grown in korea. | a scientific basis for the evaluation of the risk to public health arising from excessive dietary intake of nitrate in korea is provided. the nitrate () and nitrite () contents of various vegetables (chinese cabbage, radish, lettuce, spinach, soybean sprouts, onion, pumpkin, green onion, cucumber, potato, carrot, garlic, green pepper, cabbage and allium tuberosum roth known as crown daisy) are reported. six hundred samples of 15 vegetables cultivated during different seasons were analysed for ni ... | 2003 | 12888387 |
| isolation and antihypertensive effect of angiotensin i-converting enzyme (ace) inhibitory peptides from spinach rubisco. | four new inhibitory peptides for angiotensin i-converting enzyme (ace), that is, mrwrd, mrw, lripva, and iaykpag, were isolated from the pepsin-pancreatin digest of spinach rubisco with the use of hplc. ic(50) values of individual peptides were 2.1, 0.6, 0.38, and 4.2 microm, respectively. mrw and mrwrd had an antihypertensive effect after oral administration to spontaneously hypertensive rats. maximal reduction occurred 2 h after oral administration of mrw, whereas mrwrd showed maximal decrease ... | 2003 | 12903942 |
| species-specific differences of the spectroscopic properties of p700: analysis of the influence of non-conserved amino acid residues by site-directed mutagenesis of photosystem i from chlamydomonas reinhardtii. | we applied optical spectroscopy, magnetic resonance techniques, and redox titrations to investigate the properties of the primary electron donor p700 in photosystem i (ps i) core complexes from cyanobacteria (thermosynechococcus elongatus, spirulina platensis, and synechocystis sp. pcc 6803), algae (chlamydomonas reinhardtii cc2696), and higher plants (spinacia oleracea). remarkable species-specific differences of the optical properties of p700 were revealed monitoring the (3p700-p700) and (p700 ... | 2003 | 12933812 |
| crystal structure of the psbq protein of photosystem ii from higher plants. | the smallest extrinsic polypeptide of the water-oxidizing complex (psbq) was extracted and purified from spinach (spinacia oleracea) photosystem ii (psii) membranes. it was then crystallized in the presence of zn(2+) and its structure was determined by x-ray diffraction at 1.95-a resolution using the multi-wavelength anomalous diffraction method, with the zinc as the anomalous scatterer. the crystal structure shows that the core of the protein is a four-helix bundle, whereas the amino-terminal p ... | 2003 | 12949587 |
| domain analysis of the chloroplast polynucleotide phosphorylase reveals discrete functions in rna degradation, polyadenylation, and sequence homology with exosome proteins. | the molecular mechanism of mrna degradation in the chloroplast consists of sequential events, including endonucleolytic cleavage, the addition of poly(a)-rich sequences to the endonucleolytic cleavage products, and exonucleolytic degradation. in spinach chloroplasts, the latter two steps of polyadenylation and exonucleolytic degradation are performed by the same phosphorolytic and processive enzyme, polynucleotide phosphorylase (pnpase). an analysis of its amino acid sequence shows that the prot ... | 2003 | 12953107 |
| refolding from denatured inclusion bodies, purification to homogeneity and simplified assay of mgdg synthases from land plants. | in plant cells, the synthesis of monogalactosyldiacylglycerol (mgdg) is catalyzed within plastid envelope membranes by mgd proteins. mgdg synthesis was also reported in apicomplexan parasites, a phylum of protists harbouring a plastid that proved essential for the parasite survival. mgd activity is therefore a potent target for herbicidal and anti-parasitic molecules. in this study, we describe a detailed in vitro refolding protocol for denatured recombinant mgd accumulated in inclusion bodies f ... | 2003 | 12963344 |
| dithiol oxidant and disulfide reductant dynamically regulate the phosphorylation of light-harvesting complex ii proteins in thylakoid membranes. | light-induced phosphorylation of light-harvesting chlorophyll a/b complex ii (lhcii) proteins in plant thylakoid membranes requires an activation of the lhcii kinase via binding of plastoquinol to cytochrome b(6)f complex. however, a gradual down-regulation of lhcii protein phosphorylation occurs in higher plant leaves in vivo with increasing light intensity. this inhibition is likely to be mediated by increasing concentration of thiol reductants in the chloroplast. here, we have determined the ... | 2003 | 12970473 |
| the proteins of green leaves. v. a cytoplasmic nucleoprotein from spinach and tobacco leaves. | 1953 | 13129275 | |
| determination of nitrates by a novel ion chromatographic method: occurrence in leafy vegetables (organic and conventional) and exposure assessment for italian consumers. | a novel ion chromatographic method to detect nitrates in vegetables was developed, and the nitrate contents in green salad (a mixture of endive and prickly lettuce), lettuce, chicory, rocket and spinach were determined from italian markets in 1996-2002. these leaf vegetables were included because they are currently supposed to provide most of the nitrate intake in the typical italian diet. the highest content of nitrate was detected in chicory (6250 mg kg(-1)) and rocket (6120 mg kg(-1)), which ... | 2003 | 13129772 |
| lecithinase systems in sugar beet, spinach, cabbage, and carrot. | 1954 | 13190466 | |
| the nucleic acid content of chloroplasts isolated from spinach and tobacco leaves. | 1957 | 13471039 | |
| [determination of histamine, n-dimethylhistamine, n-acetylhistamine and acetylcholine in spinacia oleracea]. | 1959 | 13628473 | |
| purification and properties of a c-1 diphosphatase from spinacia oleracea. | 1959 | 13650635 | |
| correlation of structure with function in spinacea oleracea chloroplasts. | 1961 | 13732832 | |
| purification and properties of a stereospecific dihydrolipoic dehydrogenase from spinacia oleracea. | 1960 | 13797459 | |
| [action of an acetone powder of spinach leaves on hepatic steatosis and on body weight development in the rat]. | 1960 | 13825972 | |
| [biosynthesis of higher fatty acids from acetates in isolated chloroplasts of spinacia oleracea leaves]. | 2007 | 13831833 | |
| purification and properties of a dihydrolipoic dehydrogenase from spinacia oleracea. | 1963 | 13933868 | |
| chemical composition and the substructure of lamellae isolated from spinacea oleracea chloroplasts. | 1963 | 13941467 | |
| quinone and pigment composition of chloroplasts and quantasome aggregates from spinacia oleracea. | 1964 | 14114526 | |
| the biosynthesis of starch in spinach chloroplasts. | 1965 | 14275162 | |
| [yellow spotting of spinach (spinacia oleracea l.); research on central german viral strains]. | 1955 | 14388677 | |
| location, expression and orientation of the putative chlororespiratory enzymes, ndh and immutans, in higher-plant plastids. | the chloroplasts of many plants contain not only the photosynthetic electron transport chain, but also two enzymes, ndh and immutans, which might participate in a chloroplast respiratory chain. immutans encodes a protein with strong similarities to the mitochondrial alternative oxidase and hence is likely to be a plastoquinol oxidase. the ndh complex is a homologue of complex i of mitochondria and eubacteria and is considered to be a plastoquinone reductase. as yet these components have not been ... | 2003 | 14504923 |
| characterization of a protein of the plastid inner envelope having homology to animal inorganic phosphate, chloride and organic-anion transporters. | a protein from arabidopsis thaliana (l.) heynh. showing homology to animal proteins of the napi-1 family, involved in the transport of inorganic phosphate, chloride, glutamate and sialic acid, has been characterized. this protein, named antr2 (for anion transporters) was shown by chloroplast subfractionation to be localized to the plastid inner envelope in both a. thaliana and spinacia oleracea (l.). immunolocalization revealed that antr2 was expressed in the leaf mesophyll cells as well as in t ... | 2004 | 14564522 |
| maltose is the major form of carbon exported from the chloroplast at night. | transitory starch is formed in chloroplasts during the day and broken down at night. we investigated carbon export from chloroplasts resulting from transitory-starch breakdown. starch-filled chloroplasts from spinach ( spinacia oleracea l. cv. nordic iv) were isolated 1 h after the beginning of the dark period and incubated for 2.5 h, followed by centrifugation through silicone oil. exported products were measured in the incubation medium to avoid measuring compounds retained inside the chloropl ... | 2004 | 14566561 |
| optical spectra of synechocystis and spinach photosystem ii preparations at 1.7 k: identification of the d1-pheophytin energies and stark shifts. | we report and compare highly resolved, simultaneously recorded absorption and cd spectra of active photosystem ii (psii) samples in the range 440-750 nm. from an appropriately scaled comparison of spinach membrane fragment (bby) and psii core spectra, we show that key features of the core spectrum are quantitatively represented in the bby data. psii from the cyanobacterium synechocystis 6803 display spectral features in the qy region of comparable width (50-70 cm(-1) fwhm) to those seen in plant ... | 2003 | 14570479 |
| interaction of escherichia coli with growing salad spinach plants. | in this study, the interaction of a bioluminescence-labeled escherichia coli strain with growing spinach plants was assessed. through bioluminescence profiles, the direct visualization of e. coli growing around the roots of developing seedlings was accomplished. subsequent in situ glucuronidase (gus) staining of seedlings confirmed that e. coli had become internalized within root tissue and, to a limited extent, within hypocotyls. when inoculated seeds were sown in soil microcosms and cultivated ... | 2003 | 14572215 |
| role of 5-aminolevulinic acid (ala) on active oxygen-scavenging system in nacl-treated spinach (spinacia oleracea). | ala is a key precursor in the biosynthesis of porphyrins such as chlorophyll and heme, and was found to induce temporary elevations in the photosynthesis rate, apx, and cat; furthermore, treatment with ala at a low concentration might be correlated to the increase of nacl tolerance of spinach plants. the photosynthetic rate and the levels of active oxygen-scavenging system in the 3rd leaf of spinach (spinacia oleracea) plants grown by foliar treatment with 0, 0.18, 0.60 and 1.80 mmol/l 5-aminole ... | 2003 | 14593810 |
| high resolution structures of an oxidized and reduced flavoprotein. the water switch in a soluble form of (s)-mandelate dehydrogenase. | the crystal structures of a soluble mutant of the flavoenzyme mandelate dehydrogenase (mdh) from pseudomonas putida and of the substrate-reduced enzyme have been analyzed at 1.35-a resolution. the mutant (mdh-gox2) is a fully active chimeric enzyme in which residues 177-215 of the membrane-bound mdh are replaced by residues 176-195 of glycolate oxidase from spinach. both structures permit full tracing of the polypeptide backbone chain from residues 4-356, including a 4-residue segment that was d ... | 2004 | 14604988 |
| the expression of spinach glycolate oxidase (go) in e. coli and the application of go in the production of glyoxylic acid. | the complementary deoxyribonucleic acid (cdna) coding spinach glycolate oxidase (go) was amplified by reverse transcription polymerase chain reaction (rt-pcr), using the total ribonucleic acid (rna) of spinach leaves as the template, and was cloned into cloning vector pmd18-t. after the dna sequence was determined, the go gene was subcloned into escherichia coli expression vectors. sodium dodecyl sulfatepolyacrylamide gel electrophoresis (sds-page) analysis showed that recombinant go was express ... | 2003 | 14668534 |
| dissecting a cyanobacterial proteolytic system: efficiency in inducing degradation of the d1 protein of photosystem ii in cyanobacteria and plants. | a chromatography fraction, prepared from isolated thylakoids of a fatty acid desaturation mutant (fad6/desa colon, two colons km(r)) of the cyanobacterium synechocystis 6803, could induce an initial cleavage of the d1 protein in photosystem ii (psii) particles of synechocystis 6803 mutant and synechococcus 7002 wild type as well as in supercomplexes of psii-light harvesting complex ii of spinach. proteolysis was demonstrated both in darkness and in light as a reduction in the amount of full-leng ... | 2003 | 14670603 |
| substrate recognition and selectivity of plant glycerol-3-phosphate acyltransferases (gpats) from cucurbita moscata and spinacea oleracea. | stromal glycerol-3-phosphate acyltransferases (gpat) are responsible for the selective incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes, which is an important determinant of a plant's ability to tolerate chilling temperatures. the molecular mechanisms of plant chilling tolerance were elucidated by creating chimeric gpats between squash (cucurbita moscata, chilling-sensitive) and spinach (spinacea oleracea, chilling-tolerant) and the results were interpreted ... | 2004 | 14684887 |
| composition, efficacy, and safety of spinach extracts. | spinach leaves, containing several active components, including flavonoids, exhibit antioxidative, antiproliferative, and antiinflammatory properties in biological systems. spinach extracts have been demonstrated to exert numerous beneficial effects, such as chemo- and central nervous system protection and anticancer and antiaging functions. in this review article, we present a compilation of data generated in our laboratories and those of other investigators describing the chemical composition ... | 2003 | 14690799 |
| mechanism of spinach chloroplast ferredoxin-dependent nitrite reductase: spectroscopic evidence for intermediate states. | nitrite reductases found in plants, algae, and cyanobacteria catalyze the six-electron reduction of nitrite to ammonia with reduced ferredoxin serving as the electron donor. they contain one siroheme and one [4fe-4s] cluster, acting as separate one-electron carriers. nitrite is thought to bind to the siroheme and to remain bound until its complete reduction to ammonia. in the present work the enzyme catalytic cycle, with ferredoxin reduced by photosystem 1 as an electron donor, has been studied ... | 2004 | 14717606 |
| ftir spectroscopy shows structural similarities between photosystems ii from cyanobacteria and spinach. | photosystem ii (psii), an essential component of oxygenic photosynthesis, is a membrane-bound pigment protein complex found in green plants and cyanobacteria. whereas the molecular structure of cyanobacterial psii has been resolved with at least medium resolution [zouni, a., witt, h.-t., kern, j., fromme, p., krauss, n., saenger, w. & orth, p. (2001) nature (london) 409, 739-743; kamiya, n. & shen, j.r. (2003) proc. natl acad. sci. usa 100, 98-103], the structure of higher plant psii is only kno ... | 2004 | 14728683 |
| seasonal prevalence and susceptibility to agrochemicals of tyrophagus similis (acari: acaridae) in spinach buds and agricultural soil under greenhouse conditions. | seasonal prevalence of tyrophagus similis was investigated from 1997 to 1998 in two spinach greenhouses in central japan. susceptibility of t. similis to agrochemicals was also tested in the laboratory. tyrophagus similis density in the soil was low during the high temperature period from may to august. the density rapidly increased in late autumn and remained at a high level during the cool season from december to february. the number further increased in april and then rapidly decreased in may ... | 2003 | 14756393 |
| on the export of fatty acids from the chloroplast. | the model for export of fatty acids from plastids proposes that the acyl-acp (acyl carrier protein) product of de novo fatty acid synthesis is hydrolyzed in the stroma by acyl-acp thioesterases and the free fatty acid (ffa) released is then transferred to the outer envelope of the plastid where it is reactivated to acyl-coa for utilization in cytosolic glycerolipid synthesis. experiments were performed to assess whether the delivery of nascent ffa from the stroma for long chain acyl-coa synthesi ... | 2004 | 14764601 |
| comparison of survival of campylobacter jejuni in the phyllosphere with that in the rhizosphere of spinach and radish plants. | campylobacter jejuni has been isolated previously from market produce and has caused gastroenteritis outbreaks linked to produce. we have tested the ability of this human pathogen to utilize organic compounds that are present in leaf and root exudates and to survive in the plant environment under various conditions. carbon utilization profiles revealed that c. jejuni can utilize many organic acids and amino acids available on leaves and roots. despite the presence of suitable substrates in the p ... | 2004 | 14766604 |
| oxidation-reduction properties of maize ferredoxin: sulfite oxidoreductase. | oxidation-reduction titrations have been carried out on the wild-type, ferredoxin-dependent sulfite reductase from maize and two site-specific variants of the enzyme. e(m) values have been determined for the siroheme and [4fe-4s] cluster prosthetic groups of the enzyme, which titrate as independent, one-electron carriers. visible-region difference spectra suggest that reduction of the [4fe-4s] cluster significantly perturbs the spectrum of the reduced siroheme group of the enzyme. the effects of ... | 2004 | 14871491 |
| arsenic availability from chromated copper arsenate (cca)-treated wood. | lumber used to construct raised garden beds is often treated with chromated copper arsenate (cca). this project aimed to determine (i) how far as, cu, and cr had diffused away from cca-treated wood surfaces in raised garden beds under realistic conditions, (ii) the uptake of these elements by crops, and (iii) the effect of cca solution on soil bacteria. this study showed that as, cu, and cr diffuse into soil from cca-treated wood used to construct raised garden beds. to determine crop uptake of ... | 2013 | 14964372 |
| poplar peroxiredoxin q. a thioredoxin-linked chloroplast antioxidant functional in pathogen defense. | peroxiredoxins are ubiquitous thioredoxin- or glutaredoxin-dependent peroxidases, the function of which is to destroy peroxides. peroxiredoxin q, one of the four plant subtypes, is a homolog of the bacterial bacterioferritin comigratory proteins. we show here that the poplar (populus tremula x populus tremuloides) protein acts as a monomer with an intramolecular disulfide bridge between two conserved cysteines. a wide range of electron donors and substrates was tested. unlike type ii peroxiredox ... | 2004 | 14976238 |
| functional consequences of n- or c-terminal deletions of the delta subunit of chloroplast atp synthase. | mutagenesis was used to generate seven truncation mutants of the spinach (spinacia oleracea) chloroplast atp synthase delta subunit lacking 5, 11, 17, or 35 amino acid residues from the n-terminus or 3, 9, or 15 residues from the c-terminus. interactions between these mutants and all other subunits of the chloroplast atpase were investigated by a yeast two-hybrid system. the results indicate that the n-terminal deletions mainly affected interactions between the delta subunit and the other part o ... | 2004 | 14979723 |
| thioredoxin links redox to the regulation of fundamental processes of plant mitochondria. | mitochondria contain thioredoxin (trx), a regulatory disulfide protein, and an associated flavoenzyme, nadp/trx reductase, which provide a link to nadph in the organelle. unlike animal and yeast counterparts, the function of trx in plant mitochondria is largely unknown. accordingly, we have applied recently devised proteomic approaches to identify soluble trx-linked proteins in mitochondria isolated from photosynthetic (pea and spinach leaves) and heterotrophic (potato tubers) sources. applicati ... | 2004 | 14983062 |
| identification of domains on the extrinsic 23 kda protein possibly involved in electrostatic interaction with the extrinsic 33 kda protein in spinach photosystem ii. | to elucidate the domains on the extrinsic 23 kda protein involved in electrostatic interaction with the extrinsic 33 kda protein in spinach photosystem ii, we modified amino or carboxyl groups of the 23 kda protein to uncharged methyl ester groups with n-succinimidyl propionate or glycine methyl ester in the presence of a water-soluble carbodiimide, respectively. the n-succinimidyl propionate-modified 23 kda protein did not bind to the 33 kda protein associated with psii membranes, whereas the g ... | 2004 | 15009208 |
| the oxygen evolving enhancer protein 1 (oee) of photosystem ii in green algae exhibits thioredoxin activity. | a thioredoxin-like chloroplast protein of the fructosebisphosphatase-stimulating f-type, but with an unusually high molecular mass of 28 kda has previously been identified and purified to homogeneity in a fractionation scheme for resolution of the acid- and heat-stable, regular-size (12kda) thioredoxins of the unicellular green algae, scenedesmus obliquus. an apparently analogous protein of 26 kda was described in a cyanobacterium, anabaena sp., but no such large thioredoxin species f exists in ... | 2004 | 15022827 |
| cloning and salt-induced, aba-independent expression of choline mono-oxygenase in atriplex prostrata. | certain plants accumulate glycinebetaine, a type of osmoprotectant, in response to salinity. glycinebetaine is synthesized in these plants via the two-step oxidation of choline, and the first step is catalysed by choline mono-oxygenase (cmo; ec 1.14.15.7). cloned by rt-pcr and 3'-race, the cdna of atriplex prostrata cmo (apcmo) is 1669 bp in length and encodes a full-length protein of 438 amino acids. the deduced amino acid sequence of apcmo revealed a rieske-type [2fe-2s] cluster motif and a mo ... | 2004 | 15032837 |
| fractional magnesium absorption is significantly lower in human subjects from a meal served with an oxalate-rich vegetable, spinach, as compared with a meal served with kale, a vegetable with a low oxalate content. | the aim of the present study was to evaluate mg absorption from a test meal served with an oxalate-rich vegetable, spinach, as compared with a test meal served with a vegetable with a low oxalate content, kale. mg absorption was measured by a stable-isotope technique based on extrinsic labelling of the test meals and faecal monitoring of the excreted isotope labels. nine healthy adults participated in the study. the test meals were based on 100 g phytate-free white bread, served with 300 g spina ... | 2004 | 15035687 |
| [determination of low-level pesticide residues in agricultural products by ion-trap gc/ms/ms]. | the objective of this study was to elucidate the utility of ion-trap gc/ms/ms for the analysis of pesticides in extracted matrices from various agricultural products. identification and quantitative analysis of pesticides in matrices were performed by quadrupole gc/ms and ion-trap gc/ms/ms. chlorpyrifos was added to the matrix of spinach, soybean in the pod or corn, and aldrin, dieldrin, endrin, alpha-bhc, beta-bhc, gamma-bhc, delta-bhc, p,p'-ddd, p,p'-dde, o,p'-ddt and p,p'-ddt were added to ea ... | 2003 | 15038113 |
| comparative analysis of isolated cellular organelles by means of soft x-ray contact microscopy with laser-plasma source and transmission electron microscopy. | soft x-ray contact microscopy (sxcm) is, at present, a useful tool for the examination at submicrometre resolution of biological systems maintained in their natural hydrated conditions. among current x-ray-generating devices, laser-plasma sources are now easily available and, owing to their pulse nature, offer the opportunity to observe living biological samples before radiation damage occurs, even if the resolution achievable is not as high as with synchrotron-produced x-rays. to assess the pot ... | 2004 | 15049867 |
| synthesis of pyrazole derivatives and their evaluation as photosynthetic electron transport inhibitors. | two series of new pyrazoles, namely six pyrazolo[1,5-a][1,3,5]triazine-2,4-dione and four pyrazolo[1,5-c][1,3,5]thiadiazine-2-one derivatives, were synthesized as potential inhibitors of the photosynthetic electron transport chain at the photosystem ii level. the compounds were confirmed by 1h nmr, elemental, and ir analyses. their biological activity was evaluated in vivo upon both the growth of blue-green algae and the photosynthetic oxygen evolution by eukaryotic algae and in vitro as the abi ... | 2004 | 15053526 |
| functional expression of a delta12 fatty acid desaturase gene from spinach in transgenic pigs. | linoleic acid (18:2n-6) and alpha-linolenic acid (18:3n-3) are polyunsaturated fatty acids that are essential for mammalian nutrition, because mammals lack the desaturases required for synthesis of delta12 (n-6) and n-3 fatty acids. many plants can synthesize these fatty acids and, therefore, to examine the effects of a plant desaturase in mammals, we generated transgenic pigs that carried the fatty acid desaturation 2 gene for a delta12 fatty acid desaturase from spinach. levels of linoleic aci ... | 2004 | 15067141 |
| de-epoxidation of violaxanthin in light-harvesting complex i proteins. | the conversion of violaxanthin (vx) to zeaxanthin (zx) in the de-epoxidation reaction of the xanthophyll cycle plays an important role in the protection of chloroplasts against photooxidative damage. vx is bound to the antenna proteins of both photosystems. in photosystem ii, the formation of zx is essential for the ph-dependent dissipation of excess light energy as heat. the function of zx in photosystem i is still unclear. in this work we investigated the de-epoxidation characteristics of ligh ... | 2004 | 15070896 |
| pre-extraction preparation (fresh, frozen, freeze-dried, or acetone powdered) and long-term storage of fruit and vegetable tissues: effects on antioxidant enzyme activity. | activities of the antioxidant enzymes ascorbate peroxidase, catalase, dehydroascorbate reductase, glutathione reductase, guaiacol peroxidase, monodehydroascorbate reductase, and superoxide dismutase were assayed in honeydew (cucumis melo l.) fruit and spinach (spinacia oleracea l.) leaves either as fresh, frozen to -80 degrees c, frozen in liquid nitrogen, freeze-dried, or acetone powder, representing the various ways tissues are treated prior to enzyme extraction. treated tissues were analyzed ... | 2004 | 15080616 |
| antihypertensive properties of spinach leaf protein digests. | leaf protein containing approximately 50% rubisco (ribulose bisphosphate carboxylase/oxygenase) was obtained from fresh spinach leaf with the use of a simple extraction method. pepsin and pepsin-pancreatin digests of spinach leaf protein have potent angiotensin-i converting enzyme inhibitory properties with ic(50) values of 56 and 120 microg/ml, respectively. both digests of leaf protein have antihypertensive effects after oral administration to spontaneously hypertensive rats (shr) with minimum ... | 2004 | 15080624 |
| relationship between the heat tolerance of photosynthesis and the thermal stability of rubisco activase in plants from contrasting thermal environments. | inhibition of net photosynthesis (pn) by moderate heat stress has been attributed to an inability of rubisco activase to maintain rubisco in an active form. to examine this proposal, the temperature response of pn, rubisco activation, chlorophyll fluorescence, and the activities of rubisco and rubisco activase were examined in species from contrasting environments. the temperature optimum of rubisco activation was 10 degrees c higher in the creosote bush (larrea tridentata) compared with the ant ... | 2004 | 15084731 |
| effects of composting on phytotoxicity of spent pig-manure sawdust litter. | the phytotoxicity of spent pig-manure sawdust litter (spent litter) was evaluated during further composting. aqueous extracts of the spent litter were prepared by shaking the sample with water (1:10 w/v), and the toxicity of these extracts was determined on relative seed germination, relative root elongation and germination index (gi, a factor of relative seed germination and relative root elongation). the sensitivity of six plant species, namely brassica parachinensis (chinese cabbage), brassic ... | 1996 | 15093523 |
| time-resolved oxygen production by psii: chasing chemical intermediates. | photosystem ii (psii) produces dioxygen from water in a four-stepped process, which is driven by four quanta of light and catalysed by a mn-cluster and tyrosine z. oxygen is liberated during one step, coined s(3)=>s(0). chemical intermediates on the way from reversibly bound water to dioxygen have not yet been tracked, however, a break in the arrhenius plot of the oxygen-evolving step has been taken as evidence for its existence. we scrutinised the temperature dependence of (i) uv-absorption tra ... | 2004 | 15100031 |
| mechanism and importance of post-translational regulation of nitrate reductase. | in higher plants, nitrate reductase (nr) is inactivated by the phosphorylation of a conserved ser residue and binding of 14-3-3 proteins in the presence of divalent cations or polyamines. a transgenic nicotiana plumbaginifolia line (s521) has been constructed where the regulatory, conserved ser 521 of tobacco nr (corresponding to ser 534 in arabidopsis) was mutated into asp. this mutation resulted in the complete abolition of activation/inactivation in response to light/dark transitions or other ... | 2004 | 15107452 |
| ntzip antisense plants show reduced chlorophyll levels. | we have isolated and characterized a new photosynthetic tissue-specific gene ntzip (nicotiana tabacum leucine zipper) from tobacco (n. tabacum). its deduced amino acid sequence has two highly conserved regions, leucine zipper and [ex(n)dexrh](2) motifs, which are related to the gene's biochemical functions. ntzip was expressed in leaves and stems, but was not detected in roots or flowers, suggesting that its physiological functions might be associated with photosynthesis. northern blot analysis ... | 2004 | 15120117 |
| cytokinin stimulates polyribosome loading of nuclear-encoded mrnas for the plastid atp synthase in etioplasts of lupinus luteus: the complex accumulates in the inner-envelope membrane with the cf(1) moiety located towards the stromal space. | three of the nine subunits of the plastid atp synthase, including the subunit of the cf(1) moiety (gene atpc), are encoded in the nucleus. application of cytokinin to etiolated lupine seedlings induces polyribosome association of their mrnas. this appears to be specific as no such regulation was observed for messages for three ribosomal proteins. cytokinin-mediated polyribosome loading was also observed for the spinach atpc message in etiolated transgenic tobacco seedlings. analysis of various s ... | 2004 | 15125765 |
| acetyl coenzyme a concentrations in plant tissues. | despite the importance of acetyl coenzyme a in many facets of metabolism and the availability of methods for estimation of its concentration, data for acetyl-coa concentrations in plant tissues have been very scarce. a method using reversed phase hplc for the quantitative estimation of acetyl-coa was applied to a variety of plant tissues. in three different developing oilseeds the bulk acetyl-coa concentration ranged from 5 to 25 nmoles/g fresh weight. in arabidopsis thaliana leaves it was 5 nmo ... | 2004 | 15128036 |
| the 26s proteasome in garlic (allium sativum): purification and partial characterization. | the 26s proteasome (multicatalytic protease complex, mpc) was purified from fresh garlic cloves (allium sativum) to near homogeneity by ion exchange chromatography on deae-sephacel, gel filtration on sepharose-4b, and glycerol density gradient centrifugation. two alpha-type (20s proteasome "catalytic core") subunits were identified by the direct sequencing of peptide fragments (mass fingerprint analysis, mass spectrometry lab, stanford university) or the sequencing of a cloned cdna generated usi ... | 2004 | 15161196 |
| mid- to low-frequency fourier transform infrared spectra of s-state cycle for photosynthetic water oxidation in synechocystis sp. pcc 6803. | flash-induced fourier transform infrared (ftir) difference spectra for the four-step s-state cycle and the effects of global (15)n- and (13)c-isotope labeling on the difference spectra were examined for the first time in the mid- to low-frequency (1200-800 cm(-1)) as well as the mid-frequency (1700-1200 cm(-1)) regions using photosystem (ps) ii core particles from cyanobacterium synechocystis sp. pcc 6803. the difference spectra clearly exhibited the characteristic vibrational features for each ... | 2004 | 15182190 |
| heavy metal uptake by spinach leaves grown on contaminated soils with lead, mercury, cadmium, and nickel. | spinach plants were grown in soil pots contaminated with increasing mixtures of lead, mercury, cadmium, and nickel salts. plants in the control soil were grown in the absence of the heavy metals mixture. the elemental distribution of cd, ni, pb, and hg in the roots and leaves of spinach (spinacia oleracea) was determined in two stages, stage 1, after five weeks of plant growth and stage 2, after 10 weeks with full growth. under the influence of contamination of soil with the heavy metal mixtures ... | 2004 | 15186035 |
| purification and crystallization of the cytochrome b6f complex in oxygenic photosynthesis. | the cytochrome b6f complex from the thermophilic cyanobacterium mastigocladus laminosus and spinach chloroplasts has been purified as a dimeric species. it was found by electrospray ionization mass spectroscopy to contain eight and nine subunits, respectively, and dimeric masses of 217,070 and 286,454 da. the subunits common to the complex from both sources are peta (cytochrome f), b (cytochrome b6), c (rieske iron-sulfur protein), d (subunit iv), and small 3.2-4.2 kda polypeptides petg,l,m, and ... | 2004 | 15187270 |
| isolation of cp43 and cp47 photosystem ii proximal antenna complexes from plants. | a single-column method to purify the cp43 and cp47 pigment-protein complexes of photo-system (ps)ii from higher plants is presented. to validate the isolation procedure, three different species were used (spinacea oleracea, beta vulgaris, and glycine max), and the procedure worked similarly with all three. oxygen-evolving core complex obtained from highly enriched psii membrane fragments were used as the starting material. the core complex is treated with the chaotropic agent liclo4 and the noni ... | 2004 | 15187275 |
| the inhibitor dbmib provides insight into the functional architecture of the qo site in the cytochrome b6f complex. | previously [roberts, a. g., and kramer, d. m. (2001) biochemistry 40, 13407-13412], we showed that 2 equiv of the quinone analogue 2,5-dibromo-3-methyl-6-isopropylbenzoquinone (dbmib) could occupy the q(o) site of the cytochrome (cyt) b(6)f complex simultaneously. in this work, a study of electron paramagnetic resonance (epr) spectra from the oriented cyt b(6)f complex shows that the rieske iron-sulfur protein (isp) is in distinct orientations, depending on the stoichiometry of the inhibitor at ... | 2004 | 15196013 |