Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| obligate intracellular parasites do not synthesize atp. | 1986 | 3553365 | |
| the contribution of charles c. shepard to leprosy research: from the mouse footpad model to new dna technology. | 1986 | 3553793 | |
| the specificity of the immunodeficiency in lepromatous leprosy. | 1986 | 3553795 | |
| anti-mycobacterial antibodies in saliva. | 1986 | 3553796 | |
| cloning of mycobacterium leprae genes in streptomyces. | 1986 | 3553797 | |
| the development of a vaccination model using two microorganisms and its application in leprosy and leishmaniasis. | 1986 | 3553799 | |
| the karonga prevention trial--which bcg? | 1986 | 3553801 | |
| humoral immune responses to m. leprae in human volunteers vaccinated with killed, armadillo-derived m. leprae. | 1986 | 3553802 | |
| murine t-cell reactivity to cloned mycobacterium leprae antigens. | 1986 | 3553803 | |
| the carbohydrate-containing antigens of mycobacterium leprae. | 1986 | 3553804 | |
| the characterization and immunoreactivity of a 70 kd protein common to mycobacterium leprae and mycobacterium bovis (bcg). | 1986 | 3553805 | |
| characterization of the 36 k antigen of mycobacterium leprae. | 1986 | 3553806 | |
| the use of rodent models in assessing antimicrobial activity against mycobacterium leprae. | 1986 | 3553807 | |
| limited in vitro multiplication of mycobacterium leprae: application to screening potential antileprosy compounds. | 1986 | 3553808 | |
| single bacterial cell mass analysis: a rapid test method in leprosy therapy control. | 1986 | 3553809 | |
| metabolism in mycobacterium leprae: possible targets for drug action. | 1986 | 3553810 | |
| host-pathogen interaction--new in vitro drug test systems against mycobacterium leprae--possibilities and limitations. | 1986 | 3553811 | |
| isolation of environment-derived mycobacterium leprae from soil in bombay. | 1986 | 3553812 | |
| investigations into the cultivation of mycobacterium leprae. a multifactorial approach. | 1986 | 3553813 | |
| experimental leprosy in a rhesus monkey: necropsy findings. | a 6-month-old male rhesus monkey (macaca mulatta) was inoculated intravenously and intracutaneously with mycobacterium leprae obtained from a naturally infected mangabey monkey. the animal developed generalized lepromatous leprosy, and was killed for pathological examination 56 months after inoculation. lesions were observed in the skin, nasal mucosa, peripheral nerves, and peripheral lymph nodes, with relative sparing of viscera. the monkey was carefully evaluated for the retrovirus stlv-iii in ... | 1987 | 3559334 |
| circulating immune complexes in normal and immunosuppressed mice infected with mycobacterium leprae. | mycobacterium leprae infection was produced through the footpads in normal and immunosuppressed mice. circulating immune complexes were detected by specific binding test and by conglutinin binding assay for specific and total immune complexes respectively in the sera of these mice during different periods of infection. out of the total 30 samples tested from the infected groups, 3 were positive by specific binding test and 5 by conglutinin binding elisa. the implications of the findings in relat ... | 1986 | 3572093 |
| is phenolic glycolipid-1 specific for mycobacterium leprae? | 1986 | 3572110 | |
| structure of mycobacterium leprae. | 1986 | 3573941 | |
| simplification and standardization of serodiagnostic tests for leprosy based on phenolic glycolipid-i (pg-i) antigen. | 1986 | 3573946 | |
| development of a dot-elisa for detection of leprosy antigenuria under field conditions. | 1986 | 3573947 | |
| macrophage chemotaxis in mycobacterium leprae infected mice. | 1987 | 3596680 | |
| an investigation of family size and birth order as risk factors in leprosy. | to investigate if early exposure to mycobacterium leprae is a factor determining development of hansen's disease in general, or of a particular form of the disease, we have studied the sibship size and birth order distribution of 187 leprosy cases and 528 hospitalized control patients. by the greenwood-yule analysis, a small deficit of cases in the earlier birth order in the tuberculoid form was observed. such a deficit was not observed in lepromatous leprosy patients. in the multivariate analys ... | 1987 | 3655461 |
| social and demographic aspects of a leprosy epidemic on a polynesian atoll: implications of pattern. | this study reports the results of field research on a leprosy epidemic among the kapingamarangi people, polynesians living in two communities on ponape island and kapingamarangi atoll in the federated states of micronesia. the patterns of infection in the two communities are seen to replicate in detail patterns of personal mobility by age and gender and patterns of kinship and friendship relations that order people's social interactions in the communities. these patterns of demographic and socia ... | 1987 | 3655462 |
| biochemical characteristics and fatty acid compositions of some armadillo-derived mycobacteria and their relation to mycobacterium gordonae. | the long-chain components of 75 strains of mycobacteria, cultivated from mycobacterium leprae-infected or non-infected armadillos, and of eight clinical and 15 environmental isolates of m. gordonae, were compared. four major groups could be distinguished based on the presence of 10-methyloctadecanoic (tuberculostearic) and 2-methyl 3-hydroxyeicosanoic acids and secondary alcohols (2-octadecanol and 2-eicosanol). some heterogeneity was found in strains assigned to m. gordonae: the characteristic ... | 1987 | 3655731 |
| effective prophylactic measures against accidental inoculation with live mycobacterium leprae in laboratory and hospital workers. | 1987 | 3669870 | |
| hepatitis b virus (hbv) serum markers in greek leprosy patients. | the prevalence of hbsag, anti-hbs, and anti-hbc in the sera of 217 patients with the two polar types of leprosy and 382 hospital controls was studied in order to investigate the degree of exposure of greek leprosy patients to hbv and the ability of these patients to clear hbv from the blood. two distinct serological patterns were analyzed: effective exposure, characterized by the presence of one or more of the three serological markers, and active infection, characterized by the presence of hbsa ... | 1986 | 3722963 |
| ganglioside patterns in normal and lepromatous armadillo tissues. | gangliosides derived from tissues of normal and lepromatous armadillos were examined by thin-layer chromatography. the ganglioside patterns produced by the mycobacterium leprae-infected tissues varied from that of the normal tissue. although increased levels of several gangliosides were observed in the infected tissues, there was an apparent preferential increase in gm3 gangliosides as determined by column chromatography and enzymatic hydrolysis. | 1986 | 3722965 |
| the lepromin test in rhesus monkeys. | the lepromin test was studied in rhesus monkeys. six control monkeys which had not been inoculated with mycobacterium leprae, six monkeys with experimentally induced leprosy, and nine monkeys which had been inoculated with m. leprae but had not developed leprosy were evaluated with 1x, 10x, and 15x lepromin a, with 1x and 10x lepromin m (mangabey monkey derived), with 1x and 25x purified inactivated m. leprae, and with an armadillo mock lepromin. we found that the lepromin test is useful in rhes ... | 1986 | 3746044 |
| lepromatous placentitis and intrauterine fetal infection in lepromatous nine-banded armadillos (dasypus novemcinctus). | three pregnant lepromatous armadillos along with the three sets of four fetuses and their placentae were studied histopathologically. mycobacterium leprae were present in the decidual tissue, trophoblastic cells which line the chorionic villi, and in the cells that form the internal structure of the villi. acid-fast organisms were also seen in the spleens of three fetuses. congenital infection is clearly possible in leprosy in the armadillo and may also occur in humans. | 1987 | 3795871 |
| adoptive cell transfer of resistance to mycobacterium leprae infections in mice. | cells were transferred from mice intradermally vaccinated with killed mycobacterium leprae to sublethally irradiated recipients. unseparated cells from lymph nodes or spleens of m. leprae vaccinated mice were found to cause significant inhibition of the growth of a subsequent m. leprae challenge in mouse footpads for up to 26 weeks after vaccination. vaccination with live bcg and cells transferred from bcg-vaccinated mice caused no significant inhibition of m. leprae growth in mouse footpads. ce ... | 1985 | 3876183 |
| hla-linked control of predisposition to lepromatous leprosy. | in a study of the relation between hla and lepromatous leprosy, hla haplotype segregation was analyzed in 28 families with multiple cases of different types of leprosy. the inheritance of hla-dr2, hla-dr3, and hla-mt1, which had previously been shown to be associated with susceptibility to leprosy or with a leprosy type, was analyzed separately. segregation occurred in a significantly nonrandom fashion in both polar tuberculoid leprosy and lepromatous leprosy. this finding indicated hla-encoded ... | 1985 | 3880800 |
| susceptibility of mycobacterium leprae to the bactericidal activity of mouse peritoneal macrophages and to hydrogen peroxide. | macrophages from athymic nude mice were infected in vitro with mycobacterium leprae to study the intracellular fate of this organism. using the proportional bactericidal test, we have shown that the viability of m. leprae declines rapidly within these macrophages, although results of clearance experiments demonstrate that live and killed organisms are cleared at comparable rates. we have also shown that m. leprae is susceptible to the bactericidal effects of hydrogen peroxide and we suggest that ... | 1985 | 3881593 |
| manifestations of experimental leprosy in the armadillo. | three experiments, using different routes and doses of infection, were conducted using 42 armadillos. thirty-six of them developed generalized disease. there is no significant sex or age difference in susceptibility. route and dose of infection make very little difference in the disease prevalence except that the intravenous administration of a large dose reduces the period of development of generalized disease. it is quite possible that in armadillos the resistance to the disease is partly gene ... | 1985 | 3882008 |
| molecular analysis of dna and construction of genomic libraries of mycobacterium leprae. | molecular analysis of dna from mycobacterium leprae, "mycobacterium lufu," and mycobacterium vaccae has demonstrated that the g + c (guanine plus cytosine) contents of the dnas are 56, 61, and 65%, respectively, and that the genome sizes are 2.2 x 10(9), 3.1 x 10(9), and 3.1 x 10(9) daltons, respectively. because of the significant differences in both g + c content and genome size among m. leprae, "m. lufu," and m. vaccae dnas, these species are not related, although hybridization experiments un ... | 1985 | 3882664 |
| [diagnostic test systems based on the immunoenzyme method in leprosy]. | diagnostic test systems for the detection of igg and igm to mycobacterium leprae in the blood sera of leprosy patients and armadillos experimentally infected with m. leprae have been developed on the basis of the indirect immunoperoxidase assay. the possibility has been shown of prognosing the activity of the leprotic process in leprosy patients and the results of the experimental infection of armadillos by the dynamic increase of antibody reactions with the development of the infection. | 1985 | 3885647 |
| leprosy and the eye: a review. | 1985 | 3886906 | |
| mycobacterium leprae in seminal fluid: a case report. | 1985 | 3887080 | |
| the search for new drugs for the treatment of leprosy. | 1985 | 3887081 | |
| accessory cell function of cells of the mononuclear phagocyte system isolated from mycobacterial granulomas. | epithelioid cells from bcg-induced granulomas and macrophages from mycobacterium leprae-induced granulomas were examined for their ability to act as accessory cells for t-cell proliferation to mitogen (con a) and antigen (ppd). the granuloma cells were separated on a facs using monoclonal antibody specific to guinea pig macrophages. epithelioid cells (which are ia negative) were able to support proliferation to con a but not to antigen. cultures containing ia positive granuloma macrophages from ... | 1985 | 3888411 |
| an attempt to infect turtles (kinosternon leucostonum) with mycobacterium leprae and m. lepraemurium. | 1985 | 3888712 | |
| intracellular multiplication of leprosy-derived mycobacteria in schwann cells of dorsal root ganglion cultures. | organized nerve cultures of dorsal root ganglia from neonatal mice were infected with mycobacterium leprae, the causative agent of leprosy. a significant multiplication of the acid-fast bacilli was observed within the schwann cell component of the culture. the growth of these bacilli was sensitive to antileprosy drugs and was not observed directly in bacteriological media. these organisms were brightly stained with the monoclonal antibody to phenolic glycolipid-i, a m. leprae-specific marker. th ... | 1985 | 3889048 |
| alternate radiolabeled markers for detecting metabolic activity of mycobacterium leprae residing in murine macrophages. | this study demonstrated the utility of using 4% naoh as a murine macrophage cell-solubilizing agent to discriminate between host macrophage metabolism and that of intracellular mycobacterium leprae. a 4% concentration of naoh had no deleterious effect on labeled mycobacteria. thereby, alternate radiolabeled indicators of the metabolic activity of intracellular m. leprae could be experimented with. significant incorporation of 14c-amino acid mixture, [14c]leucine, [14c]uridine, and carrier-free 3 ... | 1985 | 3889052 |
| use of a polysulfone membrane support for immunochemical analysis of a glycolipid from mycobacterium leprae. | polysulfone membranes have been used as a solid support for chromatography and immunoblotting of phenolic glycolipid i from mycobacterium leprae. these membranes have an advantage over other supports such as nitrocellulose and silica gel in that very little non-specific background binding of antibodies occurs and assays can readily be carried out with igm antibodies from human sera. an example of use of the polysulfone chromatography system for detection of phenolic glycolipid i in sera from lep ... | 1985 | 3889165 |
| leprosy in a mangabey monkey--naturally acquired infection. | naturally acquired leprosy was detected in an otherwise normal "sooty" mangabey monkey (cercocebus atys). this animal was imported from west africa in 1975 and developed clinical symptoms of leprosy in 1979. histopathologic findings were those of subpolar-lepromatous to borderline-lepromatous leprosy in the ridley-jopling classification. the disease was progressive, with crippling neuropathic deformities of the hands and feet. the disease regressed under specific therapy. the etiologic agent was ... | 1985 | 3889184 |
| dds-resistant leprosy. | 1985 | 3889186 | |
| drug sensitivity testing of m. leprae. | 1985 | 3889187 | |
| abstracts: first u.s. hansen's disease research conference. national hansen's disease center, carville, louisiana, u.s.a, 13-15 august 1954. | 1985 | 3889188 | |
| serological survey of leprosy and control subjects by a monoclonal antibody-based immunoassay. | a serological antibody competition test (sact) using a murine anti-mycobacterium leprae-specific monoclonal antibody (ml04) has been evaluated in 96 untreated leprosy patients and 128 control subjects. the test is based on the competitive inhibition by antibodies from human test sera of the binding of the specific 125i-ml04 murine monoclonal antibody to m. leprae soluble antigen coated microtiter plates. along the spectrum of the disease, sact positivity was observed in 100% of ll-bl, 87.5% of b ... | 1985 | 3889189 |
| comparative study on the thin-layer chromatographic pattern of methyl mycolates of m. leprae and related species. | the thin-layer chromatographic (tlc) pattern of methyl mycolates of mycobacterium leprae has been compared with the lipids extracted from m. leprae-infected tissue material and also with those of other cultivable mycobacterial species. it has been found that m. leprae-derived methyl mycolates give two spots, namely, alpha- and keto-derivatives, after charring the tlc plates with k2cr2o7/h2so4 spray. lipids extracted from infected tissue material also show spots with the same rf values when compa ... | 1985 | 3889190 |
| an electron microscopic study on macrophages and lymphocytes in lepromatous and borderline leprosy. | we describe how macrophages are activated and phagocytose mycobacteria in lepromatous leprosy. the differentiation of macrophages into epithelioid cells and into giant cells in borderline leprosy is shown. close apposition between macrophages and lymphocytes is seen in those areas where mycobacteria disintegrate inside macrophages. | 1985 | 3889192 |
| an electron microscopic study of the small cutaneous vessels in lepromatous leprosy. | the small dermal vessels play an important role for the propagation of mycobacterium leprae. bacteria can multiply in the endothelial cells as well as in the pericytes and can enter the blood stream from the endothelial cells and the interstitial space from the pericytes. m. leprae can be phagocytosed by blood monocytes. | 1985 | 3889193 |
| electron microscopic findings of the peripheral nerve lesions of nude mice inoculated with m. leprae. | peripheral nerve lesions of nude mice inoculated with mycobacterium leprae were examined from 1 year to 1 year and 8 months post-inoculation. leprosy bacilli and intracytoplasmic foamy structures were found in the perineurial cells, endothelial cells of capillaries, schwann cells, and axons of the myelinated nerve fibers inside the lepromas of foot pads. in the proximal portion of the sciatic nerve, bacilli were found chiefly in the macrophages of the endoneurial space. bacilli were not found in ... | 1985 | 3889194 |
| cultivable mycobacteria isolated from organs of armadillos uninoculated and inoculated with mycobacterium leprae. | mycobacteria were cultivated from 16 out of 32 samples of tissues from armadillos inoculated with mycobacterium leprae. three out of 7 samples from non-inoculated armadillos held in captivity were also positive for cultivable mycobacteria. some isolated strains belonged to the m. avium-intracellulare-scrofulaceum complex, while others were identified as m. gordonae and m. terrae. unclassified mycobacteria were isolated from m. leprae-inoculated armadillos only. taxonomic studies confirmed that t ... | 1985 | 3890695 |
| essential fatty acids in the plasma phospholipids of patients with leprosy. | plasma phospholipid essential fatty acids were investigated in 40 patients with leprosy and 40 controls. a significant reduction in linoleic acid was found in the leprosy patients, with an increase in its metabolite dihomo-gamma-linolenic acid. no difference was found between patients with multibacillary and paucibacillary leprosy. patients treated for less than 6 months were found to have low levels of linoleic acid and high levels of dihomo-gamma-linolenic and arachidonic acid compared with pa ... | 1985 | 3890922 |
| study on the micromorphology of mycobacterium leprae. | the micromorphology of mycobacterium leprae is described. after fixation with osmium tetroxide supplemented with calcium ions, the cell wall was seen to be composed of three layers; the cytoplasmic membrane exhibited the architecture of an elementary membrane. the mesosomes were best visualized after fixation with glutaraldehyde; they were sometimes in contact with the nuclear equivalent. only one sort of phosphate body was found. the nucleoid was best visualized after fixation with osmium tetro ... | 1985 | 3893332 |
| immunochemical characterization of a protein associated with mycobacterium leprae cell wall. | a panel of nine monoclonal antibodies to mycobacterium leprae were used to characterize a protein antigen of the bacillus. two monoclonal antibodies (ivd8 and iiie9) were specific for m. leprae and reacted with an epitope (cwpa) present on a protein molecule associated with the cell wall fraction of m. leprae. this protein, designated cell wall-associated protein (cwp), lost its immunoreactivity upon treatment with trypsin and had an apparent molecular weight of 65,000, though additional lower-m ... | 1985 | 3894233 |
| [osteitis leprosa multiplex cystica--an early symptom of lepromatous leprosy]. | bone lesions of the finger in a 20-year-old leprosy patient are described to show their diagnostic value. the aetiological differences between specific (caused by mycobacterium leprae itself) and unspecific osseal changes are morphologically noticeable in morbus hansen. the x-ray picture shows cystic lightening by granulomatous destruction and distal absorption of the bone. these lesions are typical, and radiological investigations might be the first step in the diagnosis of leprosy. in our case ... | 1985 | 3894289 |
| changes in circulating antibody levels to the major phenolic glycolipid during erythema nodosum leprosum in leprosy patients. | circulating antibody levels to the phenolic glycolipid from mycobacterium leprae and soluble m. leprae antigens were monitored before, during and following enl episodes in 12 patients. it was observed that during enl reaction, there was a fall in circulating antibody levels to the phenolic glycolipid but not to the soluble antigens from m. leprae. when the patients had recovered from their enl reactions, the anti-glycolipid antibody levels usually increased again to levels similar to those obser ... | 1985 | 3894538 |
| phagocytic and bactericidal activities of macrophages from mycobacterium leprae-infected normal and immunosuppressed mice. | phagocytic and bactericidal activities were studied in mycobacterium leprae-infected normal (ni) and thymectomized/irradiated (tri) mice at different time periods. no significant differences were seen in the phagocytic activity for staphylococcus aureus at 3, 6, and 9 months in the normal infected (ni) and normal control (nc) mice. a slight but significant decrease in the phagocytosis of s. aureus was seen in the tri as compared to the ni group at 3 months which recovered at 6 months. phagocytos ... | 1985 | 3894539 |
| electron microscopic findings of transverse fission of m. leprae by freeze-etching methods. | the structures of multiplication by transverse fission found in the lepromas and livers of nude mice and nine-banded armadillos inoculated with mycobacterium leprae isolated from human lepromas, a nine-banded armadillo with naturally acquired leprosy-like disease, and a nine-banded armadillo inoculated with m. leprae isolated from the mangabey monkey with naturally acquired leprosy infection are described. the images of multiplication by transverse fission of m. leprae were almost always found i ... | 1985 | 3894540 |
| an unusual case of untreated polar lepromatous leprosy associated with rare m. leprae. | 1985 | 3894542 | |
| the effect of mycobacterium leprae on pha- and ppd-induced inhibition of leukocyte migration in leprosy patients. | 1985 | 3894846 | |
| rapid, radiometric in vitro assay for the evaluation of the anti-leprosy activity of clofazimine and its analogues. | 1985 | 3894849 | |
| leprosy bacillus outwitted. | 1985 | 3894978 | |
| genes for the major protein antigens of the leprosy parasite mycobacterium leprae. | leprosy, a chronic infectious disease afflicting between 10 and 15 million people, is caused by the obligate intracellular parasite mycobacterium leprae. although m. leprae was the first identified bacterial pathogen of man, basic biochemical, immunological, diagnostic and therapeutic investigations have been severely limited because it remains one of the few human pathogens that have not been cultured in vitro. an m. leprae recombinant dna expression library was constructed to provide a source ... | 1985 | 3894979 |
| isoenzymes of mycobacteria. ii. relevance of ldh zymograms in taxonomy and identification. | the cell free extracts of mycobacteria namely m. kansasii m. avium, m. tuberculosis, bcg (glaxo), m. gastri, m. phlei, m. smegmatis, m. vaccae, m. strain w., m. scrofulaceum, m. gordonae, m. nonchromogenicum e. coli, staph, aureus, and m. leprae infected skin have been electrophoresed and stained for ldh activity. normal skin tissue was also taken as control. it was found that all the organisms tested showed distinct species specific ldh isoenzyme patterns. there was no extra band but an aberran ... | 1985 | 3897403 |
| absence of mycobactin in mycobacterium leprae; probably a microbe dependent microorganism implications. | ferric mycobactins were prepared from mycobacterium phlei. mycobacterium avium--intracellulare a and h, isolated respectively from armadillo and human leprosy specimens. attempts were made to extract mycobactin from host grown m. leprae cells. the crude ferric mycobactin extracts were tested for growth supporting effect on the mycobactin dependent m. paratuberculosis strain atcc 19698. mycobactins prepared from m. phlei and the two m. avium--intracellulare strains had growth promoting effect on ... | 1985 | 3897405 |
| thymopentin treatment in patients with chemotherapy-resistant lepromatous leprosy. | leprosy is a chronic infectious disease caused by mycobacterium leprae; it is chiefly involving the skin and peripheral nerves. in lepromatous leprosy there are widespread loose infiltrates with m. leprae multiplying extensively in the skin macrophages and schwann cells of peripheral nerves. such patients reveal a decrease of circulating t helper cells, which is still more pronounced in the cutaneous lesions. due to the ever increasing bacterial resistance to classical dapsone and combined chemo ... | 1985 | 3898294 |
| detection of phenolic glycolipid i in sera from patients with lepromatous leprosy. | 1985 | 3900237 | |
| results from cation and mass fingerprint analysis of single cells and from atp measurements of m. leprae for drug sensitivity testing: a comparison. | the physiologic states of mycobacterium leprae isolated from patient biopsies were studied using single cell mass spectrometry by laser microprobe mass analysis (lam-ma) and atp bioluminescence assay. the changes in the physiologic state of m. leprae after the patients had been treated with dapsone (dds) monotherapy were also studied. the shift of the low intracellular na+, k+-ratio of untreated m. leprae cells to higher values under dds therapy, as measured from a limited number of single bacte ... | 1985 | 3900243 |
| comparison of radiometric macrophage assay and the mouse foot pad infection for the evaluation of mycobacterium leprae sensitivity/resistance to dapsone. | studies were undertaken in three independent centers to compare the newer, in vitro radiometric macrophage (m phi) assay with the conventional mouse foot pad infection for the diagnosis of dapsone resistance. results obtained on 12 bacilliferous patients showed good concordance in both assays. one strain diagnosed as sensitive in the m phi assay was found to be resistant in the mouse foot pad. three mycobacterium leprae strains considered to be partially resistant in the mouse infection behaved ... | 1985 | 3900244 |
| in vivo responses to mycobacterium leprae: antigen presentation, interleukin-2 production, and immune cell phenotypes in naturally occurring leprosy lesions. | to investigate the immune defect in lepromatous leprosy we studied immune cell phenotypes, lymphocyte activation states, and interleukin-2 (il-2) production in naturally occurring leprosy skin lesions. mouse hybridoma monoclonal antibodies reacting with the il-2 receptor (anti-tac), unbound il-2 (dms-1), antigen-presenting langerhans' cells (okt6) and the okt4-leu3 and okt8 t-lymphocyte subpopulations were used with indirect horseradish peroxidase and alkaline phosphatase techniques on frozen bi ... | 1985 | 3900245 |
| microscopic findings of delayed reactions elicited by the skin test reagent leprosin a derived from m. leprae. | punch biopsies taken 12, 24, 48, and 72 hours after skin testing with leprosin a have been used to prepare ultrathin sections for the identification and enumeration of infiltration cells. the study was performed on small numbers of both healthy persons and leprosy patients with various forms of the disease living in india. similar cells were found to infiltrate both positive and negative responses to the skin test reagent, although there were quantitative differences. the most striking findings ... | 1985 | 3900246 |
| immunity to leprosy. 1. the proliferative response of murine t lymphocytes to mycobacterium leprae. | a microculture assay is described for measuring the response of murine t lymphocytes to mycobacterium leprae antigens. mice were immunized with m. leprae in freund's incomplete adjuvant at the base of the tail, and after five days lymphocytes from draining lymph nodes were harvested and cultured with m. leprae antigens. radioactive thymidine uptake was used to quantitate the antigen-induced proliferation of the lymphocytes. the effects of cell density and antigen concentration on the kinetics of ... | 1985 | 3900249 |
| electron microscopic observations of cell wall and cytoplasmic membrane in murine and human leprosy bacilli. | the cell wall and the cytoplasmic membrane of mycobacterium lepraemurium in murine lepromas and m. leprae in human skin lepromas were studied in ultrathin serial sections at the electron microscopic level. the cell wall in m. lepraemurium was composed of three layers: an innermost electron-dense layer, an intermediate electron-transparent zone, and a thin outermost electron-dense layer. the fine structure of the cell wall in m. leprae was slightly different. in general, the cytoplasmic membrane ... | 1985 | 3900250 |
| use of gas chromatography to differentiate mycobacterium leprae from cultivable armadillo-derived mycobacteria, m. avium/intracellulare, and m. lepraemurium by analysis of secondary alcohols. | two long-chain secondary alcohols, 2-octadecanol and 2-eicosanol, were demonstrated by gas chromatography in hydrolysates of mycobacterium avium/intracellulare, in cultivable, armadillo-derived mycobacteria, and in m. lepraemurium grown in vivo, but they were not found in purified suspensions of m. leprae isolated from experimentally infected armadillos. gas chromatographic analysis of these alcohols constitutes a method for rapid detection and quantification of contaminating mycobacteria in pre ... | 1985 | 3900251 |
| isolation of characteristic glycolipids possibly included in spherical droplets around m. leprae. | the main purpose of this work was to isolate the components in acetone soluble lipids of lepromas of the nine-banded armadillo by high performance liquid chromatography (hplc), and then to examine the mass spectrometric characteristics of the two peaks (molecular weights 2000 and 1600) found by hplc. the armadillo had been inoculated with mycobacterium leprae isolated from a mangabey monkey with naturally acquired leprosy. according to the results of hplc, gas liquid chromatographic and mass spe ... | 1985 | 3900252 |
| incorporation of 32p into the phospholipids of mycobacterium leprae. | 1985 | 3901877 | |
| an enzyme immunoassay titrating igm antibody against phenolic glycolipid for diagnosis of lepromatous leprosy. | 1985 | 3902635 | |
| further characterization including preliminary chemical analysis of antigen mlw1 from mycobacterium leprae. | mlw1, an antigen preparation from mycobacterium leprae previously shown to have a high content of m. leprae antigen no. 7 (ml7), was found to contain the typical cell wall constituents arabinose, galactose and mannose. the fatty acid composition of mlw1 was largely comparable to that of undisrupted cells. the capacity of mlw1 to stimulate lymphocytes was further studied. good correlation was obtained between the in vitro lymphocyte responses to mlw1 and human-derived m. leprae, indicating simila ... | 1985 | 3902662 |
| the free lipids of mycobacterium leprae harvested from experimentally infected nine-banded armadillos. | the free lipids of a sample of mycobacterium leprae were extracted by a procedure designed to produce separate non-polar and polar fractions. the composition of these lipids was analysed semi-quantitatively by five special thin-layer chromatographic systems covering the total range of mycobacterial lipid polarities. in order of increasing polarity, the major lipids were dimycocerosates of phthiocerol a, phthiocerol b and phthiodiolone a, glycosyl phenolphthiocerol dimycocerosates and phospholipi ... | 1985 | 3903039 |
| quantitative comparison of the mycolic and fatty acid compositions of mycobacterium leprae and mycobacterium gordonae. | the mycolic and fatty acids of three samples each of mycobacterium leprae and mycobacterium gordonae were compared. acids released by whole-organism alkaline hydrolysis were converted to 4-nitrobenzyl esters and mycolic acids were further derivatized to t-butyldimethylsilyl ethers. thin-layer chromatography of the derivatized long-chain extracts showed that all three m. leprae preparations contained so-called alpha-mycolates and ketomycolates but that the m. gordonae samples had a methoxymycolat ... | 1985 | 3903040 |
| the action of deoxyfructose serotonin on intracellular bacilli and on host response in leprosy. | 1985 | 3903407 | |
| serum demyelinating factors and adjuvant-like activity of mycobacterium leprae: possible causes of early nerve damage in leprosy. | 1985 | 3903408 | |
| presence of soluble, mycobacterium leprae-derived antigen in the inflammatory exudate of reactional lepromatous leprosy. | 1985 | 3903409 | |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 49-1985. a 25-year-old haitian man with hansen's disease, painful subcutaneous nodules, fever, and hypesthesia. | 1985 | 3903507 | |
| leprosy vaccine. large-scale trials begin in india. | 1985 | 3903512 | |
| identification of lymphocyte subsets in leprosin a positive sites following vaccination. | 1985 | 3905743 | |
| [clinical therapy of leprosy and acid-fast bacilli in the nervous system]. | 1985 | 3905745 | |
| mycobacterium leprae-induced alterations in macrophage fc receptor expression and monocyte-lymphocyte interaction in familial contacts of leprosy patients. | macrophage fc receptor expression and monocyte-lymphocyte interaction in the presence of mycobacterium leprae were examined in familial contacts of leprosy patients. defective m phi functions similar to those of borderline and lepromatous patients could be observed in approximately 71% of consanguineous contacts and 43% of spouses of index patients. although the values in the latter group were markedly lower than those of the consanguineous contacts, they tended to be higher than those of normal ... | 1985 | 3906853 |
| vaccines against leprosy. | 1985 | 3907649 | |
| isolation of the mycobacterium leprae-specific glycolipid antigen, phenolic glycolipid-i, from formalin-fixed human lepromatous liver. | a mycobacterium leprae-specific phenolic glycolipid antigen was purified from formalin-fixed liver preserved from an advanced lepromatous leprosy patient. its chemical and immunological properties were compared with those of phenolic glycolipid-i obtained from m. leprae-infected armadillo liver. based on the findings that the glycolipids from the two sources have the same thin-layer chromatographic properties, infrared absorption spectrum, sugar composition, and seroreactivity, we conclude that ... | 1985 | 3908478 |
| immunological potential of a cultivable mycobacterial strain m. habana against leprosy bacillus in mouse foot pad. | a strain of a typical mycobacteria m. habana originally afforded protection against m. tuberculosis challenge in mice, was tested for its immunological potential against leprosy bacillus in the mouse foot pad. the vaccine strain m. habana has arrested the growth of m. leprae into the mouse foot pad better than bcg (phipps) and unvaccinated control. | 1985 | 3908575 |
| vaccination of leprosy patients and healthy contacts. | 1985 | 3908576 | |
| mouse foot-pad studies with m. leprae--effect of desoxy fructo serotonin (dfs) and related compounds. | mouse foot-pad experiments were carried out to study the effects of dfs and related compounds on the multiplication of m. leprae. of the 25 cases clinically suspected dapsone resistance, 8 were found resistant and 14 sensitive to dapsone by mouse foot-pad experiments. six were resistant to dfs and 16 were sensitive. desoxy fructo 5-hydroxy tryptophane as well as nutrition antileprosy (nal) diet were also found effective in suppressing the growth of m. leprae in mouse foot-pad. of the two liposol ... | 1985 | 3908578 |