Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| elimination of polyamine n-acetylation and regulatory engineering improved putrescine production by corynebacterium glutamicum. | corynebacterium glutamicum has been engineered for production of the polyamide monomer putrescine or 1,4-diaminobutane. here, n-acetylputrescine was shown to be a significant by-product of putrescine production by recombinant putrescine producing c. glutamicum strains. a systematic gene deletion approach of 18 (putative) n-acetyltransferase genes revealed that the cg1722 gene product was responsible for putrescine acetylation. the encoded enzyme was purified and characterized as polyamine n-acet ... | 2015 | 25449016 |
| acyl-coa sensing by fasr to adjust fatty acid synthesis in corynebacterium glutamicum. | corynebacterium glutamicum, like mycobacterium tuberculosis, is a member of the corynebacteriales, which have linear fatty acids and as branched fatty acids the mycolic acids. we identified accd1 and fasa as key genes of fatty acid synthesis, encoding the β-subunit of the acetyl-coa carboxylase and a type-i fatty acid synthase, respectively, and observed their repression during growth on minimal medium with acetate. we also identified the transcriptional regulator fasr and its binding sites in t ... | 2014 | 25449109 |
| absolute quantification of corynebacterium glutamicum glycolytic and anaplerotic enzymes by qconcat. | the soil bacterium corynebacterium glutamicum is one of the best-studied production hosts for industrial biotechnology, and it is primarily used for the large-scale production of essential amino acids, such as l-lysine. for rational strain development, detailed knowledge of intracellular protein concentration is crucial to determine metabolic capacities and limitations. we developed a qconcat approach for the accurate absolute quantification of key enzymes of c. glutamicum glycolysis and anapler ... | 2015 | 25451015 |
| economically enhanced succinic acid fermentation from cassava bagasse hydrolysate using corynebacterium glutamicum immobilized in porous polyurethane filler. | an immobilized fermentation system, using cassava bagasse hydrolysate (cbh) and mixed alkalis, was developed to achieve economical succinic acid production by corynebacterium glutamicum. the c. glutamicum strains were immobilized in porous polyurethane filler (ppf). cbh was used efficiently as a carbon source instead of more expensive glucose. moreover, as a novel method for regulating ph, the easily decomposing nahco3 was replaced by mixed alkalis (naoh and mg(oh)2) for succinic acid production ... | 2014 | 25463799 |
| engineering microorganisms based on molecular evolutionary analysis: a succinate production case study. | evolution has resulted in thousands of species possessing similar metabolic enzymes with identical functions that are, however, regulated by different mechanisms. it is thus difficult to select optimal gene to engineer novel or manipulated metabolic pathways. here, we tested the ability of molecular evolutionary analysis to identify appropriate genes from various species. we calculated the fraction of synonymous substitution and the effective number of codons (enc) for nine genes stemming from g ... | 2014 | 25469170 |
| the crystal structures of apo and camp-bound glxr from corynebacterium glutamicum reveal structural and dynamic changes upon camp binding in crp/fnr family transcription factors. | the cyclic amp-dependent transcriptional regulator glxr from corynebacterium glutamicum is a member of the super-family of crp/fnr (cyclic amp receptor protein/fumarate and nitrate reduction regulator) transcriptional regulators that play central roles in bacterial metabolic regulatory networks. in c. glutamicum, which is widely used for the industrial production of amino acids and serves as a non-pathogenic model organism for members of the corynebacteriales including mycobacterium tuberculosis ... | 2014 | 25469635 |
| rho and rnase play a central role in fmn riboswitch regulation in corynebacterium glutamicum. | riboswitches are rna elements that regulate gene expression in response to their ligand. although these regulations are thought to be performed without any aid of other factors, recent studies suggested the participation of protein factors such as transcriptional termination factor rho and rnase in some riboswitch regulations. however, to what extent these protein factors contribute to the regulation was unclear. here, we studied the regulatory mechanism of the flavin mononucleotide (fmn) ribosw ... | 2014 | 25477389 |
| a chromosomally encoded t7 rna polymerase-dependent gene expression system for corynebacterium glutamicum: construction and comparative evaluation at the single-cell level. | corynebacterium glutamicum has become a favourite model organism in white biotechnology. nevertheless, only few systems for the regulatable (over)expression of homologous and heterologous genes are currently available, all of which are based on the endogenous rna polymerase. in this study, we developed an isopropyl-β-d-1-thiogalactopyranosid (iptg)-inducible t7 expression system in the prophage-free strain c. glutamicum mb001. for this purpose, part of the de3 region of escherichia coli bl21(de3 ... | 2014 | 25488698 |
| production of 2-ketoisocaproate with corynebacterium glutamicum strains devoid of plasmids and heterologous genes. | 2-ketoisocaproate (kic), the last intermediate in l-leucine biosynthesis, has various medical and industrial applications. after deletion of the ilve gene for transaminase b in l-leucine production strains of corynebacterium glutamicum, kic became the major product, however, the strains were auxotrophic for l-isoleucine. to avoid auxotrophy, reduction of ilve activity by exchanging the atg start codon of ilve by gtg was tested instead of an ilve deletion. the resulting strains were indeed able t ... | 2015 | 25488800 |
| improvement of l-citrulline production in corynebacterium glutamicum by ornithine acetyltransferase. | in this study, corynebacterium glutamicum atcc 13032 was engineered to produce l-citrulline through a metabolic engineering strategy. to prevent the flux away from l-citrulline and to increase the expression levels of genes involved in the citrulline biosynthesis pathway, the argininosuccinate synthase gene (argg) and the repressor gene (argr) were inactivated. the engineered c. glutamicum atcc 13032 ∆argg ∆argr (cit 2) produced higher amounts of l-citrulline (5.43 g/l) compared to the wildtype ... | 2015 | 25492493 |
| functional characterization of corynebacterium glutamicum mycothiol s-conjugate amidase. | the present study focuses on the genetic and biochemical characterization of mycothiol s-conjugate amidase (mca) of corynebacterium glutamicum. recombinant c. glutamicum mca was heterologously expressed in escherichia coli and purified to apparent homogeneity. the molecular weight of native mca protein determined by gel filtration chromatography was 35 kda, indicating that mca exists as monomers in the purification condition. mca showed amidase activity with mycothiol s-conjugate of monobromobim ... | 2014 | 25514023 |
| production of l-ornithine from sucrose and molasses by recombinant corynebacterium glutamicum. | sucrose and molasses are attractive raw materials for industrial fermentation. although corynebacterium glutamicum shows sucrose-utilizing activity, sucrose or molasses is only a fraction of carbon source used in the fermentation medium in most works. an engineered c. glutamicum strain was constructed for producing l-ornithine with sucrose or molasses as a sole carbon source by transferring mannheimia succiniciproducens β-fructofuranosidase gene (sacc). the engineered strain, c. glutamicum δape6 ... | 2015 | 25527174 |
| transcriptional regulation of the vanillate utilization genes (vanabk operon) of corynebacterium glutamicum by vanr, a padr-like repressor. | corynebacterium glutamicum is able to utilize vanillate, the product of lignin degradation, as the sole carbon source. the vanillate utilization components are encoded by the vanabk operon. the vana and vanb genes encode the subunits of vanillate o-demethylase, converting vanillate to protocatechuate, while vank is the specific vanillate transporter. the vanabk operon is regulated by a padr-type repressor, vanr. heterologous gene expression and variations of the vanr open reading frame revealed ... | 2015 | 25535273 |
| a third glucose uptake bypass in corynebacterium glutamicum atcc 31833. | in corynebacterium glutamicum, the phosphoenolpyruvate-dependent sugar phosphotransferase system (pts) has long been the only known glucose uptake system, but we recently found suppressor mutants emerging from a pts-negative strain of c. glutamicum atcc 31833 on glucose agar plates, and identified two alternative potential glucose uptake systems, the myo-inositol transporters encoded by iolt1 and iolt2. the expression of either gene renders the pts-negative strain wtδptsh capable of growing on g ... | 2015 | 25549619 |
| involvement of the nadh oxidase-encoding noxa gene in oxidative stress responses in corynebacterium glutamicum. | corynebacterium glutamicum orf ncgl0328, designated noxa, encodes an nadh oxidase enzyme. the noxa gene, which was preferentially expressed in the log growth phase, was found to be under the control of the whca, whcb, and whce genes, which play regulatory roles in cells under oxidative stress. while noxa transcription was minimal in whce-deleted mutant cells (δwhce) during growth, its transcription was maximal even in the stationary phase in δwhca cells. the transcription levels of noxa in δwhcb ... | 2015 | 25549620 |
| exploring lysine riboswitch for metabolic flux control and improvement of l-lysine synthesis in corynebacterium glutamicum. | riboswitch, a regulatory part of an mrna molecule that can specifically bind a metabolite and regulate gene expression, is attractive for engineering biological systems, especially for the control of metabolic fluxes in industrial microorganisms. here, we demonstrate the use of lysine riboswitch and intracellular l-lysine as a signal to control the competing but essential metabolic by-pathways of lysine biosynthesis. to this end, we first examined the natural lysine riboswitches of eschericia co ... | 2015 | 25575181 |
| metabolic engineering of an atp-neutral embden-meyerhof-parnas pathway in corynebacterium glutamicum: growth restoration by an adaptive point mutation in nadh dehydrogenase. | corynebacterium glutamicum uses the embden-meyerhof-parnas pathway of glycolysis and gains 2 mol of atp per mol of glucose by substrate-level phosphorylation (slp). to engineer glycolysis without net atp formation by slp, endogenous phosphorylating nad-dependent glyceraldehyde-3-phosphate dehydrogenase (gapdh) was replaced by nonphosphorylating nadp-dependent glyceraldehyde-3-phosphate dehydrogenase (gapn) from clostridium acetobutylicum, which irreversibly converts glyceraldehyde-3-phosphate (g ... | 2015 | 25576602 |
| expression of a bacterial 3-dehydroshikimate dehydratase reduces lignin content and improves biomass saccharification efficiency. | lignin confers recalcitrance to plant biomass used as feedstocks in agro-processing industries or as source of renewable sugars for the production of bioproducts. the metabolic steps for the synthesis of lignin building blocks belong to the shikimate and phenylpropanoid pathways. genetic engineering efforts to reduce lignin content typically employ gene knockout or gene silencing techniques to constitutively repress one of these metabolic pathways. recently, new strategies have emerged offering ... | 2015 | 25583257 |
| copper homeostasis-related genes in three separate transcriptional units regulated by csor in corynebacterium glutamicum. | in corynebacterium glutamicum r, csor acts as a transcriptional repressor not only of the cognate copa-csor operon but also of the copz1-copb-cgr_0126 operon. it is predicted that copa and copb encode p-type atpases for copper efflux and copz1 encodes a metallochaperone. here, a csor-binding motif was found upstream of another copz-like gene, copz2, and the in vitro binding of the csor protein to its promoter was confirmed. the monocistronic copz2 transcript was upregulated by excess copper in a ... | 2015 | 25592736 |
| thermal and solvent stress cross-tolerance conferred to corynebacterium glutamicum by adaptive laboratory evolution. | reinforcing microbial thermotolerance is a strategy to enable fermentation with flexible temperature settings and thereby to save cooling costs. here, we report on adaptive laboratory evolution (ale) of the amino acid-producing bacterium corynebacterium glutamicum under thermal stress. after 65 days of serial passage of the transgenic strain gly3, in which the glycolytic pathway is optimized for alanine production under oxygen deprivation, three strains adapted to supraoptimal temperatures were ... | 2015 | 25595768 |
| metabolic engineering of corynebacterium glutamicum for methanol metabolism. | methanol is already an important carbon feedstock in the chemical industry, but it has found only limited application in biotechnological production processes. this can be mostly attributed to the inability of most microbial platform organisms to utilize methanol as a carbon and energy source. with the aim to turn methanol into a suitable feedstock for microbial production processes, we engineered the industrially important but nonmethylotrophic bacterium corynebacterium glutamicum toward the ut ... | 2015 | 25595770 |
| modification of chimeric (2s, 3s)-butanediol dehydrogenase based on structural information. | a chimeric (2s, 3s)-butanediol dehydrogenase (clbdh) was engineered to have the strict (s)-configuration specificity of the (2s, 3s)-bdh (bslbdh) derived from brevibacterium saccharolyticum as well as the enzymatic stability of the (2r, 3s)-bdh (kpmbdh) from klebsiella pneumonia by swapping the domains of two native bdhs. however, while clbdh possesses the stability, it lacks the specificity. in order to assist in the design a bdh having strict substrate specificity, an x-ray structural analysis ... | 2014 | 25612804 |
| functional characterization of a vanillin dehydrogenase in corynebacterium glutamicum. | vanillin dehydrogenase (vdh) is a crucial enzyme involved in the degradation of lignin-derived aromatic compounds. herein, the vdh from corynebacterium glutamicum was characterized. the relative molecular mass (mr) determined by sds-page was ~51 kda, whereas the apparent native mr values revealed by gel filtration chromatography were 49.5, 92.3, 159.0 and 199.2 kda, indicating the presence of dimeric, trimeric and tetrameric forms. moreover, the enzyme showed its highest level of activity toward ... | 2015 | 25622822 |
| implication of ornithine acetyltransferase activity on l-ornithine production in corynebacterium glutamicum. | l-ornithine is an intermediate of the l-arginine biosynthetic pathway in corynebacterium glutamicum. the effect of ornithine acetyltransferase (oatase; argj) on l-ornithine production was investigated, and c. glutamicum 1006 was engineered to overproduce l-ornithine as a major product by inactivating regulatory repressor argr gene and overexpressing argj gene. a genome sequence analysis indicated that the argf gene encoding ornithine carbamoyltransferase in c. glutamicum 1006 was mutated, result ... | 2016 | 25630515 |
| whole cell bioconversion of ricinoleic acid to 12-ketooleic acid by recombinant corynebacterium glutamicum-based biocatalyst. | the biocatalytic efficiency of recombinant corynebacterium glutamicum atcc 13032 expressing the secondary alcohol dehydrogenase of micrococcus luteus nctc2665 was studied. recombinant c. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). the effects of ph, reaction temperature, and non-ionic detergent on recombinant c. glutamiucm whole cell bioconversion were examined. the determined optimal ... | 2015 | 25639721 |
| methanol-based cadaverine production by genetically engineered bacillus methanolicus strains. | methanol is regarded as an attractive substrate for biotechnological production of value-added bulk products, such as amino acids and polyamines. in the present study, the methylotrophic and thermophilic bacterium bacillus methanolicus was engineered into a microbial cell factory for the production of the platform chemical 1,5-diaminopentane (cadaverine) from methanol. this was achieved by the heterologous expression of the escherichia coli genes cada and ldcc encoding two different lysine decar ... | 2015 | 25644214 |
| comparative proteome analysis of global effect of pos5 and zwf-ppnk overexpression in l-isoleucine producing corynebacterium glutamicum ssp. lactofermentum. | corynebacterium glutamicum ssp. lactofermentum strain jhi3-156 produces l-isoleucine (ile). overexpression of the saccharomyces cerevisiae-derived nadh kinase gene (pos5) and the endogenous glucose-6-phosphate dehydrogenase and nad kinase genes (zwf-ppnk) in jhi3-156 increased ile production by 26 and 31 %, respectively. to decipher the global effect of pos5 and zwf-ppnk overexpression on ile biosynthesis, proteomic analysis was conducted. twenty-four differentially expressed proteins were ident ... | 2015 | 25650341 |
| rational engineering of multiple module pathways for the production of l-phenylalanine in corynebacterium glutamicum. | microbial production of l-phenylalanine (l-phe) from renewable sources has attracted much attention recently. in the present study, corynebacterium glutamicum 13032 was rationally engineered to produce l-phe from inexpensive glucose. first, all the l-phe biosynthesis pathway genes were investigated and the results demonstrated that in addition to arof and phea, the native ppsa, tkta, aroe and aroa, and the heterologous arol and tyrb were also the key enzymes for l-phe biosynthesis. through combi ... | 2015 | 25665502 |
| the α-glucan phosphorylase malp of corynebacterium glutamicum is subject to transcriptional regulation and competitive inhibition by adp-glucose. | α-glucan phosphorylases contribute to degradation of glycogen and maltodextrins formed in the course of maltose metabolism in bacteria. accordingly, bacterial α-glucan phosphorylases are classified as either glycogen or maltodextrin phosphorylase, glgp or malp, respectively. glgp and malp enzymes follow the same catalytic mechanism, and thus their substrate spectra overlap; however, they differ in their regulation: glgp genes are constitutively expressed and the enzymes are controlled on the act ... | 2015 | 25666133 |
| corynebacterium glutamicum methionine sulfoxide reductase a uses both mycoredoxin and thioredoxin for regeneration and oxidative stress resistance. | oxidation of methionine leads to the formation of the s and r diastereomers of methionine sulfoxide (meto), which can be reversed by the actions of two structurally unrelated classes of methionine sulfoxide reductase (msr), msra and msrb, respectively. although msras have long been demonstrated in numerous bacteria, their physiological and biochemical functions remain largely unknown in actinomycetes. here, we report that a corynebacterium glutamicum methionine sulfoxide reductase a (cgmsra) tha ... | 2015 | 25681179 |
| advanced biotechnology: metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and health-care products. | corynebacterium glutamicum, escherichia coli, and saccharomyces cerevisiae in particular, have become established as important industrial workhorses in biotechnology. recent years have seen tremendous progress in their advance into tailor-made producers, driven by the upcoming demand for sustainable processes and renewable raw materials. here, the diversity and complexity of nature is simultaneously a challenge and a benefit. harnessing biodiversity in the right manner through synergistic progre ... | 2015 | 25684732 |
| analysis of acetohydroxyacid synthase variants from branched-chain amino acids-producing strains and their effects on the synthesis of branched-chain amino acids in corynebacterium glutamicum. | acetohydroxy acid synthase (ahas) controls carbon flux through the branch point and determines the relative rates of the synthesis of isoleucine, valine and leucine, respectively. however, it is strongly regulated by its end products. in this study, we characterized ahas variants from five branched-chain amino acids-producing strains. amino acid substitution occurred in both catalytic subunit and regulatory subunit. interestingly, ahas variants reduced sensitivity to feedback inhibition by branc ... | 2015 | 25697867 |
| biotransformation of oleic acid into 10-ketostearic acid by recombinant corynebacterium glutamicum-based biocatalyst. | to produce 10-ketostearic acid from oleic acid. | 2015 | 25700814 |
| overexpression of ribosome elongation factor g and recycling factor increases l-isoleucine production in corynebacterium glutamicum. | ribosome elongation factor g encoded by fusa promotes the translocation step of protein synthesis in bacteria; ribosome recycling factor encoded by frr, together with the elongation factor g, dissociates ribosomes from messenger rna after the termination of translation. both factors play important roles during protein synthesis in bacteria. in this study, we found that overexpression of fusa and/or frr led to the increase of l-isoleucine production in corynebacterium glutamicum iwj001, an l-isol ... | 2015 | 25707863 |
| interaction sites of diviva and roda from corynebacterium glutamicum. | elongation growth in actinobacteria is localized at the cell poles. this is in contrast to many classical model organisms where insertion of new cell wall material is localized around the lateral site. we previously described a role of roda from corynebacterium glutamicum in apical cell growth and morphogenesis. deletion of roda had drastic effects on morphology and growth, likely a result from misregulation of penicillin-binding proteins and cell wall precursor delivery. we identified the inter ... | 2015 | 25709601 |
| technical bias of microcultivation environments on single-cell physiology. | microscale cultivation systems are important tools to elucidate cellular dynamics beyond the population average and understand the functional architecture of single cells. however, there is scant knowledge about the bias of different microcultivation technologies on cellular functions. we therefore performed a systematic cross-platform comparison of three different microscale cultivation systems commonly harnessed in single-cell analysis: microfluidic non-contact cell traps driven by negative di ... | 2015 | 25710324 |
| expression of recombinant protein using corynebacterium glutamicum: progress, challenges and applications. | corynebacterium glutamicum (c. glutamicum) is a highly promising alternative prokaryotic host for recombinant protein expression, as it possesses several significant advantages over escherichia coli (e. coli), the currently leading bacterial protein expression system. during the past decades, several experimental techniques and vector components for genetic manipulation of c. glutamicum have been developed and validated, including strong promoters for tightly regulating target gene expression, v ... | 2016 | 25714007 |
| increased l-ornithine production in corynebacterium glutamicum by overexpression of a gene encoding a putative aminotransferase. | overexpression of the ncgl0462 open reading frame, encoding a class ii aminotransferase, was studied in conjunction with other enzymes in l-ornithine biosynthesis in an l-ornithine-producing strain. expression of the wild-type ncgl0462 open reading frame, which displayed aminotransferase activity, was amplified by placing it under the control of the glyceraldehyde 3-phosphate dehydrogenase gene promoter in the pek0 plasmid and in the genome. l-ornithine production in corynebacterium glutamicum s ... | 2015 | 25720798 |
| 4-hydroxyisoleucine production of recombinant corynebacterium glutamicum ssp. lactofermentum under optimal corn steep liquor limitation. | 4-hydroxyisoleucine (4-hil) is a nonproteinogenic amino acid that exhibits insulinotropic biological activity. here, l-isoleucine dioxygenase gene (ido) derived from bacillus thuringiensis ybt-1520 was cloned and expressed in an l-isoleucine-producing strain, corynebacterium glutamicum ssp. lactofermentum sn01, in order to directly convert its endogenous l-isoleucine (ile) into 4-hil through single-step fermentation. the effects of corn steep liquor limitation as well as ido and truncated idoδ6 ... | 2015 | 25725632 |
| structural insights into domain movement and cofactor specificity of glutamate dehydrogenase from corynebacterium glutamicum. | glutamate dehydrogenase (gdh) is an enzyme involved in the synthesis of amino acids by converting glutamate to α-ketoglutarate, and vice versa. to investigate the molecular mechanism of gdh, we determined a crystal structure of the corynebacterium glutamicum-derived gdh (cggdh) in complex with its nadp cofactor and α-ketoglutarate substrate. cggdh functions as a hexamer, and each cggdh monomer comprises 2 separate domains; a rossmann fold cofactor-binding domain and a substrate-binding domain. t ... | 2015 | 25727019 |
| response of corynebacterium glutamicum exposed to oscillating cultivation conditions in a two- and a novel three-compartment scale-down bioreactor. | the oscillatory conditions in substrate and oxygen supply that typically occur on a large (industrial) scale are usually simulated in two-compartment scale-down reactors. in this study, the performance of nutrient-limited fed-batch cultivations of corynebacterium glutamicum in a standard two-compartment reactor (two-cr) is compared to the performance in a novel three-compartment reactor (three-cr). the three-cr is designed to mimic three distinct zones of an industrial scale bioreactor that occu ... | 2015 | 25728062 |
| identification of essential tryptophan in amylomaltase from corynebacterium glutamicum. | this work aims to identify essential tryptophan residue(s) of amylomaltase from corynebacterium glutamicum (cgam) through chemical modification and site-directed mutagenesis techniques. the recombinant enzyme expressed by escherichia coli was purified and treated with n-bromosuccinimide (nbs), a modifying agent for tryptophan. a significant decrease in enzyme activity was observed indicating that tryptophan is important for catalysis. inactivation kinetics with nbs resulted in pseudo first-order ... | 2015 | 25748841 |
| mutational analysis to identify the residues essential for the inhibition of n-acetyl glutamate kinase of corynebacterium glutamicum. | n-acetyl glutamate kinase (nagk) is a key enzyme in the synthesis of l-arginine that is inhibited by its end product l-arginine in corynebacterium glutamicum (c. glutamicum). in this study, the potential binding sites of arginine and the residues essential for its inhibition were identified by homology modeling, inhibitor docking, and site-directed mutagenesis. the allosteric inhibition of nagk was successfully alleviated by a mutation, as determined through analysis of mutant enzymes, which wer ... | 2015 | 25750030 |
| a giant market and a powerful metabolism: l-lysine provided by corynebacterium glutamicum. | l-lysine is made in an exceptional large quantity of currently 2,200,000 tons/year and belongs therefore to one of the leading biotechnological products. production is done almost exclusively with mutants of corynebacterium glutamicum. the increasing l-lysine market forces companies to improve the production process fostering also a deeper understanding of the microbial physiology of c. glutamicum. current major challenges are the identification of ancillary mutations not intuitively related wit ... | 2015 | 25761623 |
| reducing lactate secretion by ldha deletion in l-glutamate- producing strain corynebacterium glutamicum gdk-9. | l-lactate is one of main byproducts excreted in to the fermentation medium. to improve l-glutamate production and reduce l-lactate accumulation, l-lactate dehydrogenase-encoding gene ldha was knocked out from l-glutamate producing strain corynebacterium glutamicum gdk-9, designated gdk-9δldha. gdk-9δldha produced approximately 10.1% more l-glutamate than the gdk-9, and yielded lower levels of such by-products as α-ketoglutarate, l-lactate and l-alanine. since dissolved oxygen (do) is one of main ... | 2014 | 25763057 |
| the corynebacterium glutamicum mycothiol peroxidase is a reactive oxygen species-scavenging enzyme that shows promiscuity in thiol redox control. | cysteine glutathione peroxidases (cysgpxs) control oxidative stress levels by reducing hydroperoxides at the expense of cysteine thiol (-sh) oxidation, and the recovery of their peroxidatic activity is generally accomplished by thioredoxin (trx). corynebacterium glutamicum mycothiol peroxidase (mpx) is a member of the cysgpx family. we discovered that its recycling is controlled by both the trx and the mycothiol (msh) pathway. after h2 o2 reduction, a sulfenic acid (-soh) is formed on the peroxi ... | 2015 | 25766783 |
| metabolic engineering of corynebacterium glutamicum atcc13869 for l-valine production. | in this study, an l-valine-producing strain was developed from corynebacterium glutamicum atcc13869 through deletion of the three genes acee, alat and ilva combined with the overexpression of six genes ilvb, ilvn, ilvc, lrp1, brnf and brne. overexpression of lrp1 alone increased l-valine production by 16-fold. deletion of the acee, alat and ilva increased l-valine production by 44-fold. overexpression of the six genes ilvb, ilvn, ilvc, lrp1, brne and brnf in the triple deletion mutant wcc003 fur ... | 2015 | 25769288 |
| the manganese-responsive regulator mntr represses transcription of a predicted zip family metal ion transporter in corynebacterium glutamicum. | manganese is an important trace element required as an enzyme cofactor and for protection against oxidative stress. in this study, we characterized the dtxr-type transcriptional regulator mntr (cg0741) of corynebacterium glutamicum atcc 13032 as a manganese-dependent repressor of the predicted zip family metal transporter cg1623. comparative transcriptome analysis of a δmntr strain and the wild type led to the identification of cg1623 as potential target gene of mntr which was about 50-fold upre ... | 2015 | 25790484 |
| lead selection and characterization of antitubercular compounds using the nested chemical library. | discovering new drugs to treat tuberculosis more efficiently and to overcome multidrug resistance is a world health priority. to find novel antitubercular agents several approaches have been used in various institutions worldwide, including target-based approaches against several validated mycobacterial enzymes and phenotypic screens. we screened more than 17,000 compounds from vichem's nested chemical library™ using an integrated strategy involving whole cell-based assays with corynebacterium g ... | 2015 | 25801335 |
| deletion of odha or pyc improves production of γ-aminobutyric acid and its precursor l-glutamate in recombinant corynebacterium glutamicum. | to enhance γ-aminobutyric acid (gaba) production in recombinant corynebacterium glutamicum, metabolic engineering strategies were used to improve the supply of the gaba precursor, l-glutamate. | 2015 | 25801673 |
| glucose consumption rate critically depends on redox state in corynebacterium glutamicum under oxygen deprivation. | rapid sugar consumption is important for the microbial production of chemicals and fuels. here, we show that overexpression of the nadh dehydrogenase gene (ndh) increased glucose consumption rate in corynebacterium glutamicum under oxygen-deprived conditions through investigating the relationship between the glucose consumption rate and intracellular nadh/nad(+) ratio in various mutant strains. the nadh/nad(+) ratio was strongly repressed under oxygen deprivation when glucose consumption was acc ... | 2015 | 25808520 |
| overexpression of the phosphofructokinase encoding gene is crucial for achieving high production of d-lactate in corynebacterium glutamicum under oxygen deprivation. | we previously reported on the impacts of the overexpression of individual genes of the glycolytic pathway encoding glucokinase (glk), glyceraldehyde phosphate dehydrogenase (gapdh), phosphofructokinase (pfk), triosephosphate isomerase (tpi), and bisphosphate aldolase (fba) on d-lactate productivity in corynebacterium glutamicum under oxygen-deprived conditions. searching for synergies, in the current study, we simultaneously overexpressed the five glycolytic genes in a stepwise fashion to evalua ... | 2015 | 25820644 |
| a tatabc-type tat translocase is required for unimpaired aerobic growth of corynebacterium glutamicum atcc13032. | the twin-arginine translocation (tat) system transports folded proteins across the cytoplasmic membrane of bacteria and the thylakoid membrane of plant chloroplasts. escherichia coli and other gram-negative bacteria possess a tatabc-type tat translocase in which each of the three inner membrane proteins tata, tatb, and tatc performs a mechanistically distinct function. in contrast, low-gc gram-positive bacteria, such as bacillus subtilis, use a tatac-type minimal tat translocase in which the tat ... | 2015 | 25837592 |
| distinct paths for basic amino acid export in escherichia coli: ybje (lyso) mediates export of l-lysine. | in escherichia coli, argo encodes an exporter for l-arginine (arg) and its toxic analogue canavanine (can), and its transcriptional activation and repression, by arg and l-lysine (lys), respectively, are mediated by the regulator argp. accordingly argo and argp mutants are can supersensitive (can(ss)). we report the identification of ybje as a gene encoding a predicted inner membrane protein that mediates export of lys, and our results confirm the previous identification with a different approac ... | 2015 | 25845847 |
| diaminopimelic acid amidation in corynebacteriales: new insights into the role of ltsa in peptidoglycan modification. | a gene named ltsa was earlier identified in rhodococcus and corynebacterium species while screening for mutations leading to increased cell susceptibility to lysozyme. the encoded protein belonged to a huge family of glutamine amidotransferases whose members catalyze amide nitrogen transfer from glutamine to various specific acceptor substrates. we here describe detailed physiological and biochemical investigations demonstrating the specific role of ltsa protein from corynebacterium glutamicum ( ... | 2015 | 25847251 |
| characterization of a flavin-containing monooxygenase from corynebacterium glutamicum and its application to production of indigo and indirubin. | to examine the role of a gene encoding flavin-containing monooxygenase (cfmo) from corynebacterium glutamicum atcc13032 when cloned and expressed in escherichia coli for the production of indigo pigments. | 2015 | 25851950 |
| redox regulation by reversible protein s-thiolation in bacteria. | low molecular weight (lmw) thiols function as thiol-redox buffers to maintain the reduced state of the cytoplasm. the best studied lmw thiol is the tripeptide glutathione (gsh) present in all eukaryotes and gram-negative bacteria. firmicutes bacteria, including bacillus and staphylococcus species utilize the redox buffer bacillithiol (bsh) while actinomycetes produce the related redox buffer mycothiol (msh). in eukaryotes, proteins are post-translationally modified to s-glutathionylated proteins ... | 2015 | 25852656 |
| unraveling the kinetic diversity of microbial 3-dehydroquinate dehydratases of shikimate pathway. | 3-dehydroquinate dehydratase (dhqase) catalyzes the conversion of 3-dehydroquinic acid to 3-dehydroshikimic acid of the shikimate pathway. in this study, 3180 prokaryotic genomes were examined and 459 dhqase sequences were retrieved. based on sequence analysis and their original hosts, 38 dhqase genes were selected for chemical synthesis. the selected dhqases were translated into new dna sequences according to the genetic codon usage bias by both escherichia coli and corynebacterium glutamicum. ... | 2015 | 25852984 |
| functional characterization of corynebacterium alkanolyticum β-xylosidase and xyloside abc transporter in corynebacterium glutamicum. | the corynebacterium alkanolyticum xylefgd gene cluster comprises the xyld gene that encodes an intracellular β-xylosidase next to the xylefg operon encoding a substrate-binding protein and two membrane permease proteins of a xyloside abc transporter. cloning of the cluster revealed a recombinant β-xylosidase of moderately high activity (turnover for p-nitrophenyl-β-d-xylopyranoside of 111 ± 4 s(-1)), weak α-l-arabinofuranosidase activity (turnover for p-nitrophenyl-α-l-arabinofuranoside of 5 ± 1 ... | 2015 | 25862223 |
| regulation of expression of soda and msra genes of corynebacterium glutamicum in response to oxidative and radiative stress. | promoters of genes encoding superoxide dismutase (soda) and peptide methionine sulfoxide reductase (msra) from cory-nebacterium glutamicum were cloned and sequenced. promoter region analysis of soda-msra was unable to identify putative sites of fixed eventual regulators except for possible sites of fixed oxyr and integra-tion host factor. a study of the regulation of these genes was performed using the lacz gene of escherichia coli as a reporter placed under the control of sequences downstream o ... | 2015 | 25867357 |
| involvement of the tetr-type regulator paar in the regulation of pristinamycin i biosynthesis through an effect on precursor supply in streptomyces pristinaespiralis. | pristinamycin i (pi), produced by streptomyces pristinaespiralis, is a streptogramin type b antibiotic, which contains two proteinogenic and five aproteinogenic amino acid precursors. pi is coproduced with pristinamycin ii (pii), a member of streptogramin type a antibiotics. the pi biosynthetic gene cluster has been cloned and characterized. however, thus far little is understood about the regulation of pi biosynthesis. in this study, a tetr family regulator (encoded by ssdg_03033) was identifie ... | 2015 | 25868645 |
| identification of the regulators binding to the upstream region of glxr in corynebacterium glutamicum. | glxr is considered as a global transcriptional regulator controlling a large number of genes having broad physiological aspects in corynebacterium glutamicum. however, the expression profile revealing the transcriptional control of glxr has not yet been studied in detail. dna affinity chromatography experiments revealed the binding of transcriptional regulators sucr, ramb, glxr, and a gntr-type protein (hereafter denoted as gntr3) to the upstream region of glxr. the binding of different regulato ... | 2015 | 25876601 |
| enhanced production of gamma-aminobutyrate (gaba) in recombinant corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded ph range. | gamma-aminobutylate (gaba) is an important chemical in pharmacetucal field and chemical industry. gaba has mostly been produced in lactic acid bacteria by adding l-glutamate to the culture medium since l-glutamate can be converted into gaba by inherent l-glutamate decarboxylase. recently, gaba has gained much attention for the application as a major building block for the synthesis of 2-pyrrolidone and biodegradable polyamide nylon 4, which opens its application area in the industrial biotechnol ... | 2015 | 25886194 |
| development of an orthogonal fatty acid biosynthesis system in e. coli for oleochemical production. | here we report recombinant expression and activity of several type i fatty acid synthases that can function in parallel with the native escherichia coli fatty acid synthase. corynebacterium glutamicum fas1a was the most active in e. coli and this fatty acid synthase was leveraged to produce oleochemicals including fatty alcohols and methyl ketones. coexpression of fas1a with the acp/coa-reductase maqu2220 from marinobacter aquaeolei shifted the chain length distribution of fatty alcohols produce ... | 2015 | 25887638 |
| biosynthesis of l-sorbose and l-psicose based on c-c bond formation catalyzed by aldolases in an engineered corynebacterium glutamicum strain. | the property of loose stereochemical control at aldol products from aldolases helped to synthesize multiple polyhydroxylated compounds with nonnatural stereoconfiguration. in this study, we discovered for the first time that some fructose 1,6-diphosphate aldolases (frua) and tagatose 1,6-diphosphate (taga) aldolases lost their strict stereoselectivity when using l-glyceraldehyde and synthesized not only l-sorbose but also a high proportion of l-psicose. among the aldolases tested, taga from baci ... | 2015 | 25888171 |
| bioprocess automation on a mini pilot plant enables fast quantitative microbial phenotyping. | the throughput of cultivation experiments in bioprocess development has drastically increased in recent years due to the availability of sophisticated microliter scale cultivation devices. however, as these devices still require time-consuming manual work, the bottleneck was merely shifted to media preparation, inoculation and finally the analyses of cultivation samples. a first step towards solving these issues was undertaken in our former study by embedding a biolector in a robotic workstation ... | 2015 | 25888907 |
| functional characterization of a mycothiol peroxidase in corynebacterium glutamicum that uses both mycoredoxin and thioredoxin reducing systems in the response to oxidative stress. | previous studies have identified a putative mycothiol peroxidase (mpx) in corynebacterium glutamicum that shared high sequence similarity to sulfur-containing gpx (glutathione peroxidase; cysgpx). in the present study, we investigated the mpx function by examining its potential peroxidase activity using different proton donors. the mpx degrades hydrogen peroxide and alkyl hydroperoxides in the presence of either the thioredoxin/trx reductase (trx/trxr) or the mycoredoxin 1/mycothione reductase/m ... | 2015 | 25891483 |
| corynebacterium glutamicum atp-phosphoribosyl transferases suitable for l-histidine production--strategies for the elimination of feedback inhibition. | l-histidine biosynthesis in corynebacterium glutamicum is mainly regulated by l-histidine feedback inhibition of the atp-phosphoribosyltransferase hisg that catalyzes the first step of the pathway. the elimination of this feedback inhibition is the first and most important step in the development of an l-histidine production strain. for this purpose, a combined approach of random mutagenesis and rational enzyme redesign was performed. mutants spontaneously resistant to the toxic l-histidine anal ... | 2015 | 25892668 |
| reactions of cg10062, a cis-3-chloroacrylic acid dehalogenase homologue, with acetylene and allene substrates: evidence for a hydration-dependent decarboxylation. | cg10062 is a cis-3-chloroacrylic acid dehalogenase (cis-caad) homologue from corynebacterium glutamicum with an unknown function and an uninformative genomic context. it shares 53% pairwise sequence similarity with cis-caad including the six active site amino acids (pro-1, his-28, arg-70, arg-73, tyr-103, and glu-114) that are critical for cis-caad activity. however, cg10062 is a poor cis-caad: it lacks catalytic efficiency and isomer specificity. two acetylene compounds (propiolate and 2-butyno ... | 2015 | 25894805 |
| characterization of the microbiota in the guts of triatoma brasiliensis and triatoma pseudomaculata infected by trypanosoma cruzi in natural conditions using culture independent methods. | chagas disease is caused by trypanosoma cruzi, which is transmitted by triatomine vectors. the northeastern region of brazil is endemic for chagas disease and has the largest diversity of triatomine species. t. cruzi development in its triatomine vector depends on diverse factors, including the composition of bacterial gut microbiota. | 2015 | 25903360 |
| a prophage-encoded actin-like protein required for efficient viral dna replication in bacteria. | in host cells, viral replication is localized at specific subcellular sites. viruses that infect eukaryotic and prokaryotic cells often use host-derived cytoskeletal structures, such as the actin skeleton, for intracellular positioning. here, we describe that a prophage, cgp3, integrated into the genome of corynebacterium glutamicum encodes an actin-like protein, alpc. biochemical characterization confirms that alpc is a bona fide actin-like protein and cell biological analysis shows that alpc f ... | 2015 | 25916847 |
| fermentative production of the diamine putrescine: system metabolic engineering of corynebacterium glutamicum. | corynebacterium glutamicum shows great potential for the production of the glutamate-derived diamine putrescine, a monomeric compound of polyamides. a genome-scale stoichiometric model of a c. glutamicum strain with reduced ornithine transcarbamoylase activity, derepressed arginine biosynthesis, and an anabolic plasmid-addiction system for heterologous expression of e. coli ornithine decarboxylase gene spec was investigated by flux balance analysis with respect to its putrescine production poten ... | 2015 | 25919117 |
| structural insight into the thermostable nadp(+)-dependent meso-diaminopimelate dehydrogenase from ureibacillus thermosphaericus. | crystal structures of the thermostable meso-diaminopimelate dehydrogenase (dapdh) from ureibacillus thermosphaericus were determined for the enzyme in the apo form and in complex with nadp(+) and n-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid. the main-chain coordinates of the enzyme showed notable similarity to those of symbiobacterium thermophilum dapdh. however, the subunit arrangement of u. thermosphaericus dapdh (a dimer) was totally different from that of the s. thermophilum enzyme ... | 2015 | 25945579 |
| increasing succinic acid production using the pts-independent glucose transport system in a corynebacterium glutamicum pts-defective mutant. | succinic acid synthesized from glucose shows potential as a bio-based platform chemical. however, the need for a high glucose concentration, and the accompanying low yields, limit its industrial applications. despite efficient glucose uptake by the phosphotransferase system (pts), 1 mol of phosphoenolpyruvate is required for each mole of internalized glucose. therefore, a pts-defective corynebacterium glutamicum mutant was constructed to increase phosphoenolpyruvate availability for succinic aci ... | 2015 | 25952119 |
| complete genome sequence of corynebacterium glutamicum b253, a chinese lysine-producing strain. | we disclosed the complete genome sequence of corynebacterium glutamicum b253, an important lysine-producing strain in china. the genome consists a circular chromosome (3,159,203bp) and a plasmid (24,775bp), encoding 2767 protein coding genes in total. the genome contains all genes for lysine biosynthesis, and some mutations potentially relevant to lysine production were detected in comparison with sequence of other c. glutamicum. | 2015 | 25953304 |
| engineering of a hybrid route to enhance shikimic acid production in corynebacterium glutamicum. | to exploit the archaeal shikimic acid (sa) synthesis pathway toenhance sa production in corynebacterium glutamicum. | 2015 | 25967037 |
| ribosome binding site libraries and pathway modules for shikimic acid synthesis with corynebacterium glutamicum. | the shikimic acid (sa) pathway is a fundamental route to synthesize aromatic building blocks for cell growth and metabolic processes, as well as for fermentative production of various aromatic compounds. genes encoding enzymes of sa pathway are not continuous on genome and they are differently regulated. | 2015 | 25981633 |
| regulation of the pstscab operon in corynebacterium glutamicum by the regulator of acetate metabolism ramb. | the pstscab operon of corynebacterium glutamicum, which encodes an abc transport system for uptake of phosphate (pi), is induced during the pi starvation response. the two-component regulatory system phors is involved in this response, but partial pi starvation induction of pstscab in a δphors mutant indicated the involvement of additional regulator(s). regulation of pstscab also involves the global transcriptional regulator glxr. | 2015 | 26021728 |
| msccg from corynebacterium glutamicum: functional significance of the c-terminal domain. | corynebacterium glutamicum is used in microbial biotechnology for the production of amino acids, e.g., glutamate and lysine. excretion of glutamate into the surrounding medium under production conditions is mediated by msccg, an mscs-type mechanosensitive channel. in difference to most other mscs-type channel proteins, msccg carries, in addition to the n-terminal pore domain, a long c-terminal domain that amounts to about half of the size of the protein and harbors an additional transmembrane se ... | 2015 | 26033538 |
| screening currency notes for microbial pathogens and antibiotic resistance genes using a shotgun metagenomic approach. | fomites are a well-known source of microbial infections and previous studies have provided insights into the sojourning microbiome of fomites from various sources. paper currency notes are one of the most commonly exchanged objects and its potential to transmit pathogenic organisms has been well recognized. approaches to identify the microbiome associated with paper currency notes have been largely limited to culture dependent approaches. subsequent studies portrayed the use of 16s ribosomal rna ... | 2015 | 26035208 |
| construction of synthetic promoter-based expression cassettes for the production of cadaverine in recombinant corynebacterium glutamicum. | corynebacterium glutamicum is an important microorganism in the biochemical industry for the production of various platform chemicals. however, despite its importance, a limited number of studies have been conducted on how to constitute gene expression cassettes in engineered c. glutamicum to obtain desired amounts of the target products. therefore, in this study, six expression cassettes for the expression of the second lysine decarboxylase of escherichia coli, ldcc, were constructed using six ... | 2015 | 26047931 |
| metabolic engineering of escherichia coli for the production of 3-aminopropionic acid. | a novel metabolic pathway was designed for the production of 3-aminopropionic acid (3-ap), an important platform chemical for manufacturing acrylamide and acrylonitrile. using a fumaric acid producing escherichia coli strain as a host, the corynebacterium glutamicum pand gene (encoding l-aspartate-α-decarboxylase) was overexpressed and the native promoter of the aspa gene was replaced with the strong trc promoter, which allowed aspartic acid production through the aspartase-catalyzed reaction. a ... | 2015 | 26057003 |
| cmregnet-an interspecies reference database for corynebacterial and mycobacterial regulatory networks. | organisms utilize a multitude of mechanisms for responding to changing environmental conditions, maintaining their functional homeostasis and to overcome stress situations. one of the most important mechanisms is transcriptional gene regulation. in-depth study of the transcriptional gene regulatory network can lead to various practical applications, creating a greater understanding of how organisms control their cellular behavior. | 2015 | 26062809 |
| generation of mutant threonine dehydratase and its effects on isoleucine synthesis in corynebacterium glutamicum. | isoleucine synthesis is strongly regulated by its end product (isoleucine) in corynebacterium glutamicum, especially at threonine dehydratase (td) node. multiple alignments of td sequences of c. glutamicum and other sources were performed. according to the structural analysis, three td variants were constructed by site-directed mutagenesis. these td variants improved the performance of the holoenzyme. the specific activity of v140m variant was 1.5-fold higher than that of the wild-type td, where ... | 2015 | 26070433 |
| metabolic engineering of corynebacterium glutamicum for the production of itaconate. | the capability of corynebacterium glutamicum for glucose-based synthesis of itaconate was explored, which can serve as building block for production of polymers, chemicals, and fuels. c. glutamicum was highly tolerant to itaconate and did not metabolize it. expression of the aspergillus terreus cad1 gene encoding cis-aconitate decarboxylase (cad) in strain atcc13032 led to the production of 1.4mm itaconate in the stationary growth phase. fusion of cad with the escherichia coli maltose-binding pr ... | 2015 | 26100077 |
| metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by corynebacterium glutamicum. | production of the versatile bulk chemical 1,2-propanediol and the potential biofuel 1-propanol is still dependent on petroleum, but some approaches to establish bio-based production from renewable feed stocks and to avoid toxic intermediates have been described. the biotechnological workhorse corynebacterium glutamicum has also been shown to be able to overproduce 1,2-propanediol by metabolic engineering. additionally, c. glutamicum has previously been engineered for production of the biofuels e ... | 2015 | 26110019 |
| industrial production of 2,3-butanediol from the engineered corynebacterium glutamicum. | the platform chemical 2,3-butanediol (2,3-bdo) is a valuable product that can be converted into several petroleum-based chemicals via simple chemical reactions. here, we produced 2,3-bdo with the non-pathogenic and rapidly growing corynebacterium glutamicum. to enhance the 2,3-bdo production capacity of c. glutamicum, we introduced buda encoding acetolactate decarboxylase from klebsiella pneumoniae, a powerful 2,3-bdo producer. additionally, budb (encoding α-acetolactate synthase) and budc (enco ... | 2015 | 26113219 |
| two-step production of gamma-aminobutyric acid from cassava powder using corynebacterium glutamicum and lactobacillus plantarum. | production of gamma-aminobutyric acid (gaba) from crop biomass such as cassava in high concentration is desirable, but difficult to achieve. a safe biotechnological route was investigated to produce gaba from cassava powder by c. glutamicum g01 and l. plantarum gb01-21. liquefied cassava powder was first transformed to glutamic acid by simultaneous saccharification and fermentation with c. glutamicum g01, followed by biotransformation of glutamic acid to gaba with resting cells of l. plantarum g ... | 2015 | 26115763 |
| ohr protects corynebacterium glutamicum against organic hydroperoxide induced oxidative stress. | ohr, a bacterial protein encoded by the organic hydroperoxide resistance (ohr) gene, plays a critical role in resistance to organic hydroperoxides. in the present study, we show that the cys-based thiol-dependent ohr of corynebacterium glutamicum decomposes organic hydroperoxides more efficiently than hydrogen peroxide. replacement of either of the two cys residues of ohr by a ser residue resulted in drastic loss of activity. the electron donors supporting regeneration of the peroxidase activity ... | 2015 | 26121694 |
| development of a new platform for secretory production of recombinant proteins in corynebacterium glutamicum. | corynebacterium glutamicum, which has been for long an industrial producer of various l-amino acids, nucleic acids, and vitamins, is now also regarded as a potential host for the secretory production of recombinant proteins. to harness its potential as an industrial platform for recombinant protein production, the development of an efficient secretion system is necessary. particularly, regarding protein production in large-scale bioreactors, it would be appropriate to develop a secretory express ... | 2016 | 26134574 |
| specific γ-aminobutyric acid decomposition by gabp and gabt under neutral ph in recombinant corynebacterium glutamicum. | corynebacterium glutamicum that expresses the exogenous l-glutamate decarboxylase (gad) gene can synthesize γ-aminobutyric acid (gaba). to prevent gaba decomposition in the recombinant c. glutamicum gad strain, gaba uptake and the gaba shunt pathway were blocked. | 2015 | 26140901 |
| regulatory role of charged clusters in the n-terminal domain of betp from corynebacterium glutamicum. | the trimeric transporter betp counteracts hyperosmotic stress by a fast increase in transport rate in order to accumulate the compatible solute betaine. the positively charged α-helical c-terminal domain acts as an osmosensor perceiving the increase in the internal potassium (k+) concentration. a second, still unidentified stimulus originates from stress-induced changes in the physical state of the membrane and depends on the amount of negatively charged lipids. betp possesses a 60-amino acid (a ... | 2015 | 26146128 |
| effects of phosphoenolpyruvate carboxylase desensitization on glutamic acid production in corynebacterium glutamicum atcc 13032. | phosphoenolpyruvate carboxylase (pepc) in corynebacterium glutamicum atcc13032, a glutamic-acid producing actinobacterium, is subject to feedback inhibition by metabolic intermediates such as aspartic acid and 2-oxoglutaric acid, which implies the importance of pepc in replenishing oxaloacetic acid into the tca cycle. here, we investigated the effects of feedback-insensitive pepc on glutamic acid production. a single amino-acid substitution in pepc, d299n, was found to relieve the feedback contr ... | 2016 | 26168906 |
| co-production of s-adenosyl-l-methionine and l-isoleucine in corynebacterium glutamicum. | in this study, production of s-adenosyl-l-methionine in corynebacterium glutamicum was investigated by overexpressing genes metk and vgb. compared with vector control, overexpression of metk alone in c. glutamicum atcc13032 and iwj001 increased sam production 5.11 and 11.65 times, respectively; while overexpression of metk and vgb in c. glutamicum atcc13032 and iwj001 increased sam production 5.83 and 14.95 times, respectively. further studies on iwj001/pdxw-8-metk-vgb showed that the limiting f ... | 2015 | 26215341 |
| engineering microbial cell factories: metabolic engineering of corynebacterium glutamicum with a focus on non-natural products. | corynebacterium glutamicum is the workhorse of biotechnological amino acid production. for more than 50 years amino acid producing strains of this actinomycete have been improved by classical breeding, metabolic engineering and systems and synthetic biology approaches. this review focusses mainly on recent developments on c. glutamicum strain development for non-natural products. recently, metabolite sensors have accelerated classical strain breeding. synthetic pathways for access to alternative ... | 2015 | 26216246 |
| live cell imaging of sos and prophage dynamics in isogenic bacterial populations. | almost all bacterial genomes contain dna of viral origin, including functional prophages or degenerated phage elements. a frequent but often unnoted phenomenon is the spontaneous induction of prophage elements (spi) even in the absence of an external stimulus. in this study, we have analyzed spi of the large, degenerated prophage cgp3 (187 kbp), which is integrated into the genome of the gram-positive corynebacterium glutamicum atcc 13032. time-lapse fluorescence microscopy of fluorescent report ... | 2015 | 26235130 |
| trna-dependent alanylation of diacylglycerol and phosphatidylglycerol in corynebacterium glutamicum. | aminoacyl-phosphatidylglycerol synthases (aapgss) are membrane proteins that utilize aminoacylated trnas to modify membrane lipids with amino acids. aminoacylation of membrane lipids alters the biochemical properties of the cytoplasmic membrane and enables bacteria to adapt to changes in environmental conditions. aapgss utilize alanine, lysine and arginine as modifying amino acids, and the primary lipid recipients have heretofore been defined as phosphatidylglycerol (pg) and cardiolipin. here we ... | 2015 | 26235234 |
| overexpression of methionine adenosyltransferase in corynebacterium glutamicum for production of s-adenosyl-l-methionine. | two genes encoding methionine adenosyltransferase, sam2 from saccharomyces cerevisiae and metk from corynebacterium glutamicum, were individually cloned into pdxw-8, the shuttle vector between escherichia coli and c. glutamicum, and overexpressed in e. coli dh5α and c. glutamicum atcc13032. in dh5α, both genes were overexpressed and their protein products showed the activity of methionine adenosyltransferase. in atcc13032, metk was overexpressed, its product metk showed the enzyme activity and c ... | 2016 | 26238196 |
| exploring the role of sigma factor gene expression on production by corynebacterium glutamicum: sigma factor h and fmn as example. | bacteria are known to cope with environmental changes by using alternative sigma factors binding to rna polymerase core enzyme. sigma factor is one of the targets to modify transcription regulation in bacteria and to influence production capacities. in this study, the effect of overexpressing each annotated sigma factor gene in corynebacterium glutamicum wt was assayed using an iptg inducible plasmid system and different iptg concentrations. it was revealed that growth was severely decreased whe ... | 2015 | 26257719 |
| regulation of the expression of de novo pyrimidine biosynthesis genes in corynebacterium glutamicum. | expression of pyrimidine de novo biosynthesis is downregulated by an exogenous uracil in many bacteria. in this study, we show that a putative binding motif sequence of pyrr is required for uracil-mediated repression of pyrr-lacz translational fusion. however, the uracil response was still observed in the strain with the pyrr gene deleted, implying the existence of a uracil response factor other than pyrr which also acts through the pyrr binding loop region. deletion of rho, encoding the transcr ... | 2015 | 26260458 |