Publications

[air-borne microbes in the vicinity of sewage treatment plants].	measurements of air-borne germs were conducted over one year at several sewage treatment plants with different loads and sewage treatment methods. the investigations were intended to show possible air-borne loads in the sewage treatment plants and their immediate vicinity, taking into account different climatic conditions. the results lead to the conclusion that neighboring residential areas or factories in the vicinity of sewage treatment plants are not exposed to air-borne hazards.	1986	3087103
immunocompromised animal models for the study of antibiotic combinations.	studies of various models of pseudomonas aeruginosa infections in neutropenic animals have demonstrated the superiority of the combination of beta-lactam and aminoglycoside antibiotics when compared with a single drug alone. a limited number of studies carried out in models of klebsiella pneumoniae infections in neutropenic animals have shown similar results. the efficacy of double beta-lactam combinations for the treatment of gram-negative bacillary infections has not been studied as extensivel ...	1986	3087166
evaluations of tyrosine apodecarboxylase assays for pyridoxal phosphate.	the alternate procedures used in the tyrosine apodecarboxylase assays for pyridoxal 5'-phosphate were evaluated to determine optimal conditions. two preparations of tyrosine apodecarboxylase from streptococcus faecalis were used: a cell suspension and a partially purified cell-free form. the activity of the decarboxylase was measured in two different assays using [14c]tyrosine or [3h]tyrosine as substrate. the presence of serum proteins caused greater inhibition of the assay for serum pyridoxal ...	1986	3087233
streptococcus faecalis orbital cellulitis.	a case of orbital cellulitis in which the causative organism was streptococcus faecalis--the first such case to the authors' knowledge--is reported. although hemophilus influenzae and pneumococci are most frequently encountered in orbital cellulitis, this case shows that streptococcus faecalis may also be responsible. prompt identification of the bacteria is urged.	1986	3087259
selection of a reference lot of mueller-hinton agar.	a collaborative study was undertaken to evaluate the performance of currently marketed mueller-hinton agars from seven manufacturers by replicate disk diffusion tests with standard quality control strains. identification of the manufacturers was concealed, and the resulting data were evaluated for the selection of a physical reagent standard against which the performance of future production lots would be tested and made to conform. a medium was selected which was sufficiently close to existing ...	1986	3088028
elucidation of strongyloides stercoralis by bacterial-colony displacement.	two cases of unsuspected strongyloides stercoralis infection were elucidated by the displacement of bacterial colonies on primary plating media. observation of primary plates inoculated for the diagnosis of bacterial pneumonia or gastroenteritis revealed that normal flora colonies had been moved and were aligned in a pathway, or track. this unusual colony alignment prompted us to request a stool for the examination of parasites, and s. stercoralis was found. it was concluded that the parasite is ...	1986	3088034
enterocin typing of enterococci isolated from dried infant foods.	one hundred and fifty enterococcal isolates recovered from 16 market samples of infant foods and 35 from other sources were characterized and subjected to enterocin typing with 18 indicator strains. among 150 enterococcal isolates, 114 (76%) were able to be typed by the indicator strains. although 24 enterocin patterns were observed with these enterococci, the most prevalent types were x-9, 224, and 65-603. occurrence of pattern x-9 either singly or in combination with many other types was most ...	1986	3088076
effect of amiloride on the intracellular sodium and potassium content of intact streptococcus faecalis cells in vitro.	amiloride at millimolar concentrations caused marked changes in the growth-dependent intracellular balance of na+ and k+ in streptococcus faecalis. these results, whether specific to transport processes or resulting from indirect yet unknown mechanisms, constitute the first evidence of an effect of amiloride on bacterial electrolytes.	1986	3089143
an evaluation of various working practices in shops selling raw and cooked meats.	three groups of premises (butchers' shops, supermarkets and general dealers) which sell raw and cooked meats were compared. salmonellas were not detected, but escherichia coli, and to a lesser degree staphylococcus aureus and streptococcus faecalis, were widely distributed in all three groups of premises. contamination of hands, towels and nail brushes was related to poor working practices. the presence of e. coli or str. faecalis on slicing machines was associated with contamination of meat sam ...	1986	3090137
intermittent hyperbaric oxygen therapy for reduction of mortality in experimental polymicrobial sepsis.	hyperbaric oxygen therapy has a marked beneficial effect in experimental intraabdominal sepsis. two rat models involving implantation of either rat fecal material or a mixture of pure cultures of escherichia coli, streptococcus faecalis, and bacteroides fragilis were used in this study. a death rate of 100% was obtained in control animals implanted with fecal material; with intermittent hyperbaric oxygen treatment, a death rate of only 8% was observed (p less than .005). with a mixture of pure c ...	1986	3090159
[plasmid replication and partition in e coli and sex pheromones and plasmid transfer in streptococcus faecalis].		1986	3090300
[streptococcus faecalis in urinary tract infections].		1986	3090720
cell surface hydrophobicity of group d and viridans streptococci isolated from patients with septicaemia.	sixty-three strains of group d streptococci and viridans streptococci isolated from blood cultures during a two year period were typed to the species level with conventional biochemical tests and api strep. streptococcus faecalis was the most common species isolated followed by s. sanguis, s. mitis and s. constellatus (s. milleri). one of the two isolates of s. faecium was a contamination. the reported increasing frequency of this organism and other group d and viridans streptococci as well as t ...	1986	3090804
susceptibility testing with disc diffusion for new cephalosporins: pre-diffusion revisited.	the purpose of the present study was to investigate the effect of 20-hour pre-diffusion, i.e. placing the antibiotic-containing discs on the agar for 20 hours prior to inoculation, as compared to direct diffusion, i.e. placing the discs on the agar immediately after inoculation, for the newer 3. generation cephalosporins as represented by ceftazidime and ceftriaxone. regression lines (zone sizes vs. inhibitory concentrations, as measured by plate-dilution) were constructed for three groups of ba ...	1986	3090857
rational design of substrate analogues targeted to selectively inhibit replication-specific dna polymerases.	the authors' approach to the design of dna polymerase-specific inhibitors, an approach based on the mechanism of action of 6-(p-hydroxyphenylazo)uracil, has been to disguise nucleic acid bases to mimic the purine substrates dgtp and datp. specifically, the strategy has been to synthesize bases with substituents that endow them with the capacity: to seek and react with unique features of the active site of a polymerase; and to form h bonds with complementary template pyrimidines apposing the acti ...	1986	3091344
a survived case of diabetic nonclostridial gas gangrene and the review of the literatures on microbiological findings.	nonclostridial gas gangrene in diabetics results in poor prognosis. in this paper, a survived case of diabetic nonclostridial gas gangrene caused by proteus vulgaris and streptococcus faecalis is described and microbiological findings of 31 cases of the literatures including our own case were reviewed. twenty of 31 cases died (64.5%). the microbiological findings in 30 cases revealed an average of 1.8 organism per case. predominant aerobes were e. coli (56.7%), streptococcus faecalis (16.7%), ae ...	1986	3091889
phthalocyanines as photosensitizers in biological systems and for the photodynamic therapy of tumors.		1986	3092251
comparison of bacteria in the tympanic cavity and the mastoid antrum in chronic otitis media.	materials collected from the tympanic cavity before operation and from the mastoid antrum during operation of 58 discharging ears of patients with chronic otitis media were cultured, and the bacteria in these two cavities were compared. staphylococcus aureus was the commonest organism in the tympanic cavity, and s epidermidis in the mastoid antrum. anaerobic bacteria were found only in the mastoid antrum of patient with cholesteatoma. in 32 (55 per cent) of the 58 ears examined, bacteria were de ...	1986	3092687
heterogeneity of plasmids determining high-level resistance to gentamicin in clinical isolates of streptococcus faecalis.	between november 1981 and october 1984, 48 of 3,458 clinical isolates of streptococcus faecalis at the university of michigan hospital showed high-level (greater than 2,000 micrograms/ml) resistance to gentamicin, as well as to all other clinically available aminoglycosides. thirteen percent of clinical isolates in the university of michigan hospital currently demonstrate this level of resistance. transfer of resistance to a plasmid-free streptococcal recipient was observed in filter matings for ...	1986	3092734
enterococci and antifolate antibiotics.		1986	3093220
isolation and structure of the streptococcus faecalis sex pheromone, cam373.	the streptococcus faecalis sex pheromone, cam373, which induces a mating response of donor cells harboring plasmid pam373 and is also produced by staphylococcus aureus, was isolated and its structure determined. supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 micrograms cam373 was obtained. the isolated pheromone showed activity at a concentration as low as 5 x 10(-11) m. sequence analysis indicated that cam373 was a heptap ...	1986	3093276
the oral immune system: dynamics of salivary immunoglobulin production in the inbred mouse.	in the oral cavity, salivary immunoglobulins (igs) are the principal mediators of specific immunity. using carbachol to stimulate saliva flow, we investigated, in a kinetic study, individual variations in salivary ig concentrations in 23 adult balb/c mice using an enzyme-linked immunosorbant assay (elisa). it appeared that salivary ig concentrations are highly variable in individual mice (iga: 3-81 micrograms/ml; igg: 0-2.9 micrograms/ml; igm: 0.002-0.14 microgram/ml). in individual mice stimula ...	1986	3093375
resistance of enterococci to antibiotic combinations.		1986	3093442
identification of pheromone-induced surface proteins in streptococcus faecalis and evidence of a role for lipoteichoic acid in formation of mating aggregates.	the conjugative transfer of the streptococcus faecalis plasmid pad1 is characterized by a 10,000-fold increase in frequency following sex pheromone (cad1) induction. before the increase in plasmid transfer, donor cells synthesize a proteinaceous adhesin that facilitates the formation of mating aggregates. four novel surface proteins appearing after exposure of pad1-containing cells to sex pheromone have been identified. thirty minutes after induction, a 130-kilodalton (kda) protein was detectabl ...	1986	3093466
monoclonal antibodies to cell surface antigens involved in sex pheromone induced mating in streptococcus faecalis.	hybridoma cell lines producing monoclonal antibodies to streptococcus faecalis cell surface antigens were constructed. some of the antibodies isolated were directed against surface components involved in pheromone-induced mating. this paper describes the use of the monoclonal antibodies to identify antigenically related surface components detected by immunoprecipitation and western blotting, their use in pheromone response assays, and their use as functional inhibitors in mating experiments.	1986	3093633
differential scanning calorimetry of bacteria.	thermograms obtained by differential scanning calorimetry of a range of bacteria of different heat resistances were compared. equations were derived to calculate the rate at which the numbers of viable organisms in a calorimeter decline as the temperature is raised at a constant rate. vegetative bacteria scanned at 10 degrees c min-1 showed multi-peaked thermograms with four major peaks (denoted m, n, p and q) occurring in the regions 68-73, 77-84, 89-99 and 105-110 degrees c respectively. excep ...	1986	3093634
rate of bactericidal activity for streptococcus faecalis of a new quinolone, ci-934, compared with that of amoxicillin.	the rate of bactericidal activity of a new quinolone, ci-934, was compared with that of amoxicillin for 20 strains of streptococcus faecalis. at 10 and 100 micrograms/ml, the bactericidal activity of ci-934 was more rapid at 6 h than that of amoxicillin. a paradoxical effect (a killing rate higher at 1 microgram/ml than at 100 micrograms/ml at 6 h) was observed for 19 of the 20 strains with amoxicillin and for 1 of the 20 strains with ci-934. it remains to be demonstrated whether in vivo studies ...	1986	3094437
effect of organotins on fecal pollution indicator organisms.	pure cultures of escherichia coli and streptococcus faecalis and environmental water samples were examined for the possibility that pollution involving organotin compounds could decrease the values for indicator organisms when standard methods were applied to the analysis of water samples. (ch3)2sncl2 and (ch3)3sncl decreased viable counts at about 10 to 100 mg of sn liter-1 (8.4 x 10(-5) to 8.4 x 10(-4) mol of sn liter-1), and tributyltin chloride was effective at about 0.1 to 1.0 mg of sn lite ...	1986	3094450
conjugal plasmid transfer (pam beta 1) in lactobacillus plantarum.	the streptococcal plasmid pam beta 1 (erythromycin resistance) was transferred via conjugation from streptococcus faecalis to lactobacillus plantarum and was transferred among l. plantarum strains. streptococcus sanguis challis was transformed with pam beta 1 isolated from these transconjugants, and transformants harboring intact pam beta 1 could conjugate the plasmid back to l. plantarum.	1986	3094451
microbiological basis for the use of fosfomycin trometamol as single-dose therapy for simple cystitis.	fosfomycin trometamol (fot), a new soluble salt of fosfomycin, was developed especially for single-dose treatment in uncomplicated urinary tract infections. in this study, the minimum inhibitory concentrations (mics) of fot were measured both in nutrient broth and human urine and compared with calcium fosfomycin, pipemidic acid and cotrimoxazole. a total of 300 bacterial strains of different species from recent urinary infections were studied. staphylococcus aureus showed the lowest mic (0.38 mi ...	1986	3094965
pheromones among the procaryotes.	a pheromone is a chemical excreted by an organism into the environment that acts to elicit a specific response from other organisms of the same species. the importance of pheromones in the life cycle of various species of mammals, insects, and fungi is well known. in the past decade, it has become apparent that pheromones influence the behavior and development of prokaryotes. pheromones excreted by myxobacteria, actinomycetes, and cyanobacteria elicit specific developmental responses from these ...	1986	3095030
bactericidal activity of netilmicin compared with gentamicin and streptomycin, alone and in combination with penicillin, against penicillin tolerant viridans streptococci and enterococci.	netilmicin was compared with gentamicin and streptomycin for in-vitro activity against 30 strains of penicillin-tolerant streptococci including 16 strains of enterococci. both netilmicin and gentamicin tested alone at 4 mg/l caused 99.9% kill of more than half of the 13 strains of viridans streptococci tested, whereas streptomycin, 4 mg/l, had no bactericidal effect against these strains. netilmicin, gentamicin and streptomycin tested alone at 8.0 mg/l against 10 strains of streptococcus faecali ...	1986	3095305
interactions of pyridine nucleotides with redox forms of the flavin-containing nadh peroxidase from streptococcus faecalis.	the flavin-containing nadh peroxidase of streptococcus faecalis 10c1, which catalyzes the reaction: nadh + h+ + h2o2----nad+ + 2h2o, has been purified to homogeneity in our laboratory for analyses of both its structure and redox behavior. our findings indicate that the enzyme is a tetramer of four apparently identical subunits (mr = 46,000/subunit), each containing one fad coenzyme and a second non-flavin, nonmetal redox center. there is no evidence of nonequivalence among the flavins. dithionit ...	1986	3095321
identification and partial purification of a dipeptidyl aminopeptidase from streptococcus faecalis.	a dipeptidyl aminopeptidase was identified in streptococcus faecalis jh2ss and was partially purified (approximately 245-fold) by hplc. gel filtration chromatography indicated an mr of 140 000. the partially purified enzyme exhibited a requirement for co2+. the ph optimum for the hydrolysis of l-val-l-ala-p-nitroanilide was approximately 9.5. the apparent km for this substrate was 0.22 mm. the enzyme preferentially hydrolysed x-ala-y substrates, but also utilized x-pro-y substrates, and therefor ...	1986	3095489
enhancement of streptococcus faecalis infection and complement depletion in yeast-treated mice.	enhancement of streptococcus faecalis infection and lowering of the complement level have been demonstrated in mice injected with a heat-treated suspension of baker's yeast (saccharomyces cerevisiae). the leucocyte response to the infection was not affected. the yeast preparation showed, in vitro, an intense anti-complementary activity on mouse serum and interfered with the microbial killing function of the mouse peritoneal macrophages. no significant stimulation of the growth of s. faecalis in ...	1986	3095490
effects of norfloxacin on the faecal flora in patients with complicated urinary tract infections.	the effects on the long-term use of norfloxacin, a fluorinated 4-quinolone, on the intestinal flora (both aerobic and anaerobic), were evaluated in patients suffering from recurrent complicated urinary tract infections. eight patients received norfloxacin 400 mg b.i.d. for three months, and 11 patients received 400 mg b.i.d. for one month, and then 400 mg as an evening dose for two months. the concentration of enterobacteriaceae, staphylococci, streptococcus faecalis, and yeasts in stool specime ...	1986	3095916
[current status and the development of resistance of important urinary pathogens to antibiotics in the suhl district 1970-1984].	in a retrospective study over a period from 1970 to 1984 the distribution of frequency and the behaviour of the resistance of urine germs out of more than 43,000 sendings from outpatient and hospital institutions of the county of suhl were analysed. in the period of observation the proportion of isolated e. coli redoubled and was nearly 68% in 1984. conspicuous were a decrease of the isolates of str. faecalis from 32 to 4% and an increase of problem germs of the klebsiella group to 9.7%. the res ...	1986	3095995
[aerobic bacterial flora of the nasal cavity of rabbits].	on the basis of bacteriological examinations carried out in april 1984 on 60 intranasal swabs, aerobic respiratory microbes were studied in rabbits. differences in flora between animals with and without respiratory diseases were studied. fourteen bacterial species were identified with no difference due to the pathological status. they were: bordetella bronchiseptica, staphylococcus epidermidis, streptococcus faecalis, pasteurella multocida, staphylococcus aureus, bacillus sp., branhamella catarr ...	1986	3096189
method for flow cytometric detection of listeria monocytogenes in milk.	this report describes a method for the detection of listeria monocytogenes in raw milk by flow cytometric analysis of fluorescently labeled bacterial populations. the use of immunofluorescence in combination with measures of dna content by propidium iodide labeling and size by light scattering enabled specific identification of l. monocytogenes from streptococcus faecalis, streptococcus agalactiae, streptococcus uberis, staphylococcus epidermidis, and staphylococcus hyicus. additional specific r ...	1986	3096202
in vitro activity of ci-934, a new quinolone antimicrobial, against gram-positive bacteria.	the in vitro activity of ci-934, a new quinolone antimicrobial, was compared with that of ciprofloxacin against selected gram-positive bacteria. concentrations of ci-934 required to inhibit 90% of strains (mic90) were twofold to eightfold lower than those of ciprofloxacin. with the exception of streptococcus faecium, all isolates were inhibited by ci-934 at concentrations less than or equal to 1.0 microgram/ml.	1986	3096629
[bacterial endocarditis after endoscopic polypectomy].		1986	3096804
atp-driven calcium transport in membrane vesicles of streptococcus sanguis.	calcium transport was investigated in membrane vesicles prepared from the oral bacterium streptococcus sanguis. procedures were devised for the preparation of membrane vesicles capable of accumulating 45ca2+. uptake was atp dependent and did not require a proton motive force. calcium transport in these vesicles was compared with 45ca2+ accumulation in membrane vesicles from streptococcus faecalis and escherichia coli. the data support the existence of an atp-driven calcium pump in s. sanguis sim ...	1986	3096955
reconstitution of atp-dependent calcium transport from streptococci.	membrane vesicles of three streptococcal strains (streptococcus faecalis, streptococcus lactis, and streptococcus sanguis) were extracted with octyl-beta-d-glucoside in the presence of escherichia coli lipid and glycerol. for reconstitution, the detergent extract was mixed with bath-sonicated e. coli lipid, in the presence of octyl-beta-d-glucoside, and proteoliposomes were formed by a 25-fold dilution. atp-dependent calcium accumulation by proteoliposomes was comparable to that found in parent ...	1986	3096992
pseudallescheriasis in northern britain.	five cases of pseudallescheria boydii infection are reported from the northern part of the united kingdom. none of the patients had travelled abroad. three cases of otitis in which p. boydii was associated with bacterial infections were diagnosed within a 6-month period. a lady who had diabetes mellitus presented with a pulmonary fungus ball in a pre-existent cavity caused by a previous bacterial infection. after minor trauma at the site of an old skin graft, a farmer's wife developed an ulcer o ...	1986	3097296
[does radiosterilization of the vagina occur through therapeutic radiation dosages?].	the endogenous genital flora is a major source of infections of the female genital tract, especially in patients with cervical or endometrial cancer. until recently the "radiosterilisation of the vagina", respectively a prophylactic and/or therapeutic effect of irradiation (external high voltage as well as intracavitary-radium) concerning infections was postulated in the literature. this theory was disproved in a prospective clinical and bacteriological study covering 48 patients with advanced c ...	1986	3097858
ultrastructural microbiology of infected urinary stone.	scanning and transmission electron microscopy of an antibiotic-treated infected urinary stone demonstrates a thick bacterial biofilm adhering to the surface of the stone. cultures of the crushed calculi indicated the viability of the bacteria. exopolysaccharide secreted by the bacteria develops a matrix or glycocalyx that appears to protect the biofilm-forming sessile bacteria from antibiotics.	1986	3097904
streptococcal phosphoenolpyruvate-sugar phosphotransferase system: amino acid sequence and site of atp-dependent phosphorylation of hpr.	the amino acid sequence of histidine-containing protein (hpr) from streptococcus faecalis has been determined by direct edman degradation of intact hpr and by amino acid sequence analysis of tryptic peptides, v8 proteolytic peptides, thermolytic peptides, and cyanogen bromide cleavage products. hpr from s. faecalis was found to contain 89 amino acid residues, corresponding to a molecular weight of 9438. the amino acid sequence of hpr from s. faecalis shows extended homology to the primary struct ...	1986	3098288
characterization and partial purification of a broad spectrum antibiotic as-48 produced by streptococcus faecalis.	streptococcus faecalis s-48 produces a broad spectrum antibiotic, active against gram-positive and gram-negative bacteria. this substance is produced in solid and liquid media and also in a defined basal medium. it is sensitive to protease, pronase, or trypsin, heating at 70 degrees c, and alkaline ph, but resistant to treatment with lipase, lysozyme, alkaline phosphatase, dnaase, rnaase, acidic or neutral phs, and also lower temperatures (60 degrees c). several organic solvents cause precipitat ...	1986	3098396
susceptibility of enterococci. i. inhibitory and bactericidal activity of several chemoantibiotics against streptococcus faecalis and streptococcus faecium.	the authors present a microbiological study of 100 strains of enterococcus (70 strains of streptococcus faecalis and 30 strains of streptococcus faecium) tested for susceptibility to the following antibiotics, amoxicillin, ampicillin + flucloxacillin, piperacillin, rifampicin, vancomycin, netilmicin, ofloxacin, and norfloxacin. the assessment of minimum inhibitory and minimum bactericidal concentrations of these substances indicates that all have good inhibitory activity except netilmicin, which ...	1986	3098440
susceptibility of enterococci. ii. inhibitory and bactericidal activity of drugs in combination against streptococcus faecalis and streptococcus faecium.	the microbiological utility of antibiotic combinations against streptococcus faecalis and streptococcus faecium strains was studied. the drugs used were netilmicin + amoxicillin (20 strains); netilmicin + piperacillin (20 strains); netilmicin- + vancomycin (20 strains); netilmicin + rifampicin (20 strains). netilmicin used in combination with the penicillins was advantageous against streptococcus faecalis, but not uniformly against streptococcus faecium. the combinations of netilmicin with vanco ...	1986	3098441
suppurative thrombophlebitis: correlation between pathogen and underlying disease.	we identified 29 episodes of suppurative thrombophlebitis in 27 patients admitted to a large general hospital between may 1980 and may 1984. in 25 patients, the intravenous cannulae had been in place for more than 3 days. streptococcus faecalis, pseudomonas aeruginosa or one of the enterobacteriaceae were implicated in 14 patients. all these patients had recently undergone abdominal surgery or had a major intra-abdominal inflammatory process at the time they developed thrombophlebitis. the remai ...	1986	3098696
the enterococcus.		1986	3098697
quality control limits for microdilution susceptibility tests with norfloxacin.	a multilaboratory study was designed to define quality control limits for microdilution susceptibility tests with norfloxacin. the following limits were proposed: for escherichia coli atcc 25922, 0.03 to 0.125 micrograms/ml; for pseudomonas aeruginosa atcc 27853, 1.0 to 4.0 micrograms/ml; for staphylococcus aureus atcc 29213, 0.5 to 2.0 micrograms/ml; and for streptococcus faecalis atcc 29212, 2.0 to 8.0 micrograms/ml. the latter represents a change in the previously recommended control limits.	1987	3098779
serodiagnosis of streptococcus faecalis endocarditis.		1986	3098859
use of penicillin-binding proteins for the identification of enterococci.	the results of 20 physiological and fermentation tests and examination of the penicillin-binding proteins (pbps) of 85 enterococcal strains demonstrated that the genus enterococcus could be divided into at least nine distinct species: e. faecalis, e. faecium, e. durans, e. hirae, e. avium, e. gallinarum, e. casseliflavus, e. malodoratus and e. mundtii. each species had a specific pattern of at least five pbps, with molecular masses in the range of about 40-130 kda. the pattern of pbps may be use ...	1986	3098903
[drug-resistant plasmids in clinically isolated streptococcus faecalis].		1986	3099237
[increasing active immunity in chronic recurring tonsillitis].		1986	3100407
filterability of l-forms.	we investigated the recovery of l-forms through micropore filtration. the findings indicate that l-form recovery and viability are a function of filter size and preparation technique. current methods in use for l-form isolation appear to give erroneously low results, underestimating the potential role of l-forms in human disease.	1987	3100709
[streptococcus faecalis: sensitivity to quinolones, penicillins and aminoglycosides alone or in combination].	isolates of streptococcus faecalis are moderately susceptible to quinolones, penicillins and aminoglycosides used alone. penicillin-aminoglycosides synergism is not observed against aminoglycosides high-level resistant strains. the combination of an aminoglycoside plus a quinolone was synergistic against susceptible strains of s. faecalis but not against aminoglycosides high-level resistant strains. no antagonistic effect of quinolones plus aminoglycosides or plus penicillin was observed.	1986	3101034
dispersal of a plasmid-borne chloramphenicol resistance gene in streptococcal and enterococcal plasmids.	plasmids coding for chloramphenicol resistance, five isolated from streptococci of groups a, b, and g, ten from enterococci (enterococcus faecalis, enterococcus faecium), and two from staphylococci, were tested for sequence homology with the chloramphenicol resistance gene of pip501, a 30-kb plasmid originally isolated from a group b streptococcus. the 6.3-kb hindiii fragment of pip501, known to carry the chloramphenicol resistance gene, was cloned into pbr322. a 1.6-kb portion of the cloned fra ...	1986	3101076
[mycotic intracranial aneurysm in infective endocarditis of the mitral valve].	a 20-year-old woman suffering from mitral valve endocarditis due to streptococcus faecalis infection after blunt trauma and splenectomy complained of severe headache 18 days later. cerebral angiography showed a left posterior artery aneurysm. a craniotomy was performed and the aneurysm could be successfully removed. the postoperative neurological status showed a mild transient right hemiparesis. the patient underwent mitral valve replacement with a st. jude-medical prosthesis 14 days after brain ...	1986	3101300
selective medium for isolation of saprophytic coryneform bacteria.	out of 64 test chemicals for which the toxicity was analysed on pure cultures of gram-positive and gram-negative bacteria, nalidixic acid, polymyxin b sulfate, colistin, methyl red, cefotaxim, amikacin, 2-chlorphenol, metronidazole and potassium tellurite were shown to perform best for the selective isolation of coryneform microorganisms; sodium azide, potassium thiocyanate and lithium chloride were not suitable. a new selective agar medium (npc) for coryneform bacteria containing standard agar, ...	1986	3101314
antibacterial activity of primycin against multiple strains of gram-positive bacteria.	crystalline primycin was found to be very active in broth dilution assay against staphylococcus aureus (50 strains), staphylococcus epidermidis (77 strains), streptococcus faecalis (76 strains) and one strain of listeria monocytogenes with minimal inhibitory concentrations (mic) of 0.12-0.5 microgram/ml. the activity was influenced by the ph of the liquid medium with greater activity (lower mics) at ph 8 against the majority of strains, than at ph 6 or 7.3. in disc agar diffusion assay bacillus ...	1986	3101392
[clinical trial of hapa-b in complicated urinary tract infections].	hapa-b was administered intramuscularly to 20 patients with complicated urinary tract infections to evaluate its clinical effects and safety in conformity with the estimations of efficacy of antimicrobial agents standardized by the uti committee. the overall clinical efficacy was excellent in 5 patients, moderate in 8 patients and poor in 7 patients. the effectiveness rate was 65.0%. overall clinical efficacy as classified by type of infection was 66.7% in 12 patients with a single infection, an ...	1986	3101447
[a clinical evaluation of mk-0787/mk-0791 for long-term administration in urological infections].	mk-0787 (imipenem)/mk-0791 (cilastatin sodium), a new compound of thienamycin, was administered in treatment of 35 patients (36 cases) with chronic complicated uti or for prevention of serious infections with much complicated factors. the patients were principally treated at a daily dose of 1 g for over 10 days. the efficacy rate of 26 patients who were evaluable in the early phase (4-7 days) was 88.5%, while it became up to 92.3% in the final phase judgment. as for clinical usefulness, the resu ...	1986	3101448
structural studies of a teichoic acid from streptococcus agalactiae type iii.	an unusual type of teichoic acid has been isolated from streptococcus agalactiae type iii. it has the same backbone as the lipoteichoic acid from streptococcus faecalis, but is devoid of fatty acid residues, a phosphatidyl group, and substituents in the poly(glycerol phosphate) side-chain. the following structure, with n approximately 20, was determined mainly with the aid of n.m.r. spectroscopy. (formula: see text)	1986	3102060
[sanitary microbiological verification of recreational loads on the water area of seashore beaches].		1986	3102317
serodiagnosis of streptococcus faecalis endocarditis by immunoblotting of surface protein antigens.	we describe a method for the serodiagnosis of streptococcus faecalis in infective endocarditis which could be of value in culture-negative cases. serum-grown cells of s. faecalis produced three major characteristic protein antigens (73,000, 40,000, and 37,000 molecular weight) which were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of solubilized whole cells. after electrophoretic transfer to a nitrocellulose membrane, these antigens were visualized by probing with seru ...	1987	3102551
[intergenus cell fusion between l-forms of streptococcus faecalis and staphylococcus aureus. i. attempts to induce l-forms of oral streptococci particularly s. faecalis].		1986	3102709
[intergenus cell fusion between l-form of streptococcus faecalis and staphylococcus aureus. ii. cell fusion between stable or unstable l-form cells of streptococcus faecalis and those of staphylococcus aureus].		1986	3102710
biotyping, serotyping and phage typing of streptococcus faecalis isolated from dental plaque in the human mouth.	thirty streptococcus faecalis isolates from mixed dental plaque samples were classified into four groups on the basis of biotype, tetracycline susceptibility, phage type and serotype combinations. the organisms were from patients on haemodialysis, from staff of the dialysis unit, and from controls. three biotypes were distinguished by seven biochemical tests: production of acid from inositol, sucrose and xylose; rapid or delayed production of acid from sorbitol; gelatin liquefaction; and product ...	1987	3102745
[recent trend in pathogenic bacteria of urinary tract infections--simple and complicated urinary tract infections].		1986	3102815
group d or group g streptococcal infection?		1986	3103082
bacterial and fungal growth after freezing or refrigerating parenteral nutrient solutions.	parenteral nutrient (pn) solutions were evaluated for growth of pathogenic organisms after refrigeration or freezing and then thawing. sixteen bags of hypertonic dextrose and amino acid solutions were divided into two series (refrigerated and frozen), inoculated with escherichia coli. candida albicans, staphylococcus aureus, or streptococcus faecalis, and exposed to freezing or refrigeration. the inoculum concentration was greater than would likely occur with patient contamination of the solutio ...	1987	3103431
kinetics of activation of l-lactate dehydrogenase from streptococcus faecalis by fructose 1,6-bisphosphate and by metal ions.	the lactate dehydrogenase from streptococcus faecalis is activated either by fructose 1,6-bisphosphate or by divalent cations such as mn2+ or co2+. with both types of activator, a lag is observed before attainment of the steady state rate of pyruvate reduction if the activator is added to the enzyme at the same time as the substrates. this lag can be largely abolished by preincubation of enzyme with activator before mixing with substrates. for fructose 1,6-bisphosphate (fru(1,6)p2) as the activa ...	1987	3103687
enterococci and aminoglycosides: evaluation of susceptibility and synergism of their combination with piperacillin.	some clinical isolates of enterococci were tested for susceptibility to gentamicin, tobramycin, amikacin and netilmicin. five percent of streptococcus faecalis tested demonstrated high level resistance (minimum inhibitory concentration (mic) greater than 2024 micrograms/ml) to gentamicin, tobramycin and netilmicin, while amikacin had mics greater than 128 micrograms/ml for all strains tested. since a combination of a beta-lactam and streptomycin does not produce synergism against all strains of ...	1987	3103929
isolation and structure of the sex pheromone inhibitor, ipd1, excreted by streptococcus faecalis donor strains harboring plasmid ppd1.	the sex pheromone inhibitor ipd1, which is excreted by streptococcus faecalis donor strains harboring bacteriocin plasmid ppd1 and which inhibits sex pheromone cpd1, was isolated, and its structure was determined. its molecular weight is 828, and its amino acid sequence is h-ala-leu-ile-leu-thr-leu-val-ser-oh.	1987	3104314
simultaneous determination of carbohydrates and products of carbohydrate metabolism in fermentation mixtures by hplc.	an improved procedure for separating and quantitating carbohydrates, alcohols, and organic acids in fermentation mixtures metabolized by intestinal microflora is described. the high-pressure liquid chromatographic method is efficient, reproducible, and sensitive. a column packed with cation-exchange resin in the hydrogen form, eluted isocratically with 0.028 m h2so4 at 40 degrees c separates the compounds of interest. the eluate is monitored with ultraviolet and refractive index detectors in ser ...	1987	3104385
penicillin-binding proteins in streptococcus faecalis and s. faecium.	penicillin-binding proteins (pbps) of streptococcus faecalis nctc 775, s. faecium nctc 7171 and an isolate of s. faecium (strain 37) highly resistant to beta-lactam antibiotics were visualised by autoradiography. five pbps were detected in s. faecalis nctc 775 and six in s. faecium nctc 7171. additional pbps could not be found in the resistant isolate of s. faecium. the pbp affinities of beta lactams were compared with mic values. the affinities of pbps 3 and 4 of s. faecalis nctc 775 for penici ...	1987	3104591
the enterococcus--bacterial pathogen of the 1990s.		1987	3104839
sequence of the adenine methylase gene of the streptococcus faecalis plasmid pam beta 1.		1987	3104884
nosocomial infection by gentamicin-resistant streptococcus faecalis. an epidemiologic study.	enterococci with high-level resistance to gentamicin account for 55% of clinical isolates of enterococci found in patients at the ann arbor veterans administration medical center. we prospectively studied cultures obtained from all 100 patients hospitalized from 1 december 1985 through 23 january 1986 on the surgical and thoracic intensive care units and a general medical floor. ten patients' cultures grew colonies of gentamicin-resistant enterococci--six after admission to the intensive care un ...	1987	3105375
the enterococcus: "putting the bug in our ears".	high-level resistance to gentamicin among clinical isolates of enterococci has been found with increasing frequency in recent years. in this issue, zervos and colleagues report findings from a prospective study in which they assessed the frequency of colonization and infection with such organisms at a university medical center, demonstrating probable person-to-person spread. their findings suggest that hospitals should conduct systematic screening for enterococci with high-level resistance to ge ...	1987	3105376
endocarditis after lithotripsy.		1987	3105380
[association of bacterial endocarditis and alcoholic cirrhosis in 9 patients].	ten cases of bacterial endocarditis were observed in cirrhotic patients. in 7 cases, endocarditis was due to group d streptococcus, 5 of which were streptococcus d. bovis. special features were the involvement of tricuspid valve and the involvement of two or more valves in 4 cases. surgery was necessary in the acute phase in 5 patients: cure was definitely obtained in 8 cases after a particularly long interval. a colonic lesion could have been the portal of entry in some cases. liver cirrhosis w ...	1986	3105382
[microbiological methods of pharmacokinetic research on chemotherapeutic (antibacterial and antitumor) preparations].	microbiological methods are the most accessible in clinical assay of drug concentrations in biological fluids. when a sensitive test system is available and the requirements for collection and investigation of the materials are observed such methods are applicable in studies of pharmacokinetics of both antibacterial and antitumor drugs.	1987	3105435
in vitro susceptibility of gram-positive cocci to paldimycin.	paldimycin (u-70138f) is a new antimicrobial agent with activity against gram-positive cocci. clinical isolates of staphylococci and streptococci were tested. mics were higher in mueller-hinton broth than in nutrient broth. change in ph had minimal effect on the mics in either broth. when inoculum size was varied, an inoculum effect was observed. the gram-positive cocci tested were generally more susceptible to paldimycin than to vancomycin.	1987	3105438
continuous-infusion ampicillin therapy of enterococcal endocarditis in rats.	intermittent administration of ampicillin alone has resulted in high failure rates in previously described animal models of enterococcal endocarditis. we developed a rat model of enterococcal endocarditis which permits comparison of continuous intravenous infusion of ampicillin with intramuscular therapy. continuous low-dose ampicillin infusion (450 mg/kg [body weight] per day) was compared with the same dose given intramuscularly in three divided doses and with high-dose infusion (4.5 g/kg per ...	1987	3105445
analysis of microcalorimetric curves for bacterial identification.	a numeric method is suggested for the treatment of microcalorimetric curves of bacterial growth to provide a new tool for their automatic identification. in this method the microcalorimetric curves are searched against certain reference profiles (stored in a library) by means of a cross-correlation analysis and a parametric comparison. the matching between the new curve and each reference profile is evaluated by means of a specific identification coefficient which provides an objective criterion ...	1987	3105856
monuril and modification of pathogenicity traits in resistant microorganisms.		1987	3106045
[treatment of urinary tract infections with solcourovac].	urinary tract infections are among the most common infections found in women. if recurrences or reinfections become frequent treatment can be problematic. immunisation of the patients by vaccinating them against urinary tract pathogens offers a possibility of managing these infections. in clinical trials we tested solcourovac, a new vaccine to counteract bacterial pathogens, with regard to efficacy, frequency of recurrence, and tolerance. 113 of the 118 patients included in this study with bacte ...	1987	3106132
gas chromatographic technique to simultaneously quantitate the gases produced by intestinal microorganisms from fermentation mixtures.		1987	3106391
beta-lactamase production in experimental endocarditis due to aminoglycoside-resistant streptococcus faecalis.	we used a beta-lactamase-producing (beta l+) strain of streptococcus faecalis that also had high levels of resistance to all aminoglycosides to induce experimental endocarditis in rats. the rats were treated for five or 10 days with procaine penicillin, vancomycin, gentamicin, rifampin, or ciprofloxacin (alone or in various combinations), or with penicillin plus clavulanic acid. the levels of penicillin in serum and vegetations declined rapidly in the beta l+-infected rats treated with procaine ...	1987	3106510
streptococcus faecalis sex pheromone (cad1) response: evidence that the peptide inhibitor excreted by pad1-containing cells may be plasmid determined.	streptococcus faecalis strains harboring the conjugative plasmid pad1 excrete a small peptide, iad1, which inhibits the sex pheromone cad1. studies making use of the host strain streptococcus faecium 9790, which normally does not excrete peptide pheromones, suggest that iad1 may be determined directly by pad1.	1987	3107004
enterococcus faecalis hemolysin-bacteriocin plasmids belong to the same incompatibility group.	plasmid pair coexistence was studied both among nine enterococcus faecalis hemolysin-bacteriocin (hly-bcn) plasmids, including pjh2, pad1, pam gamma 1, and pip964, and between pip964 and five r plasmids. some of the hly-bcn plasmids used were derivatives encoding resistance to erythromycin or tetracycline. the hly-bcn plasmids were incompatible with each other; 40 to 100% displacement was observed bilaterally for eight pairs and unilaterally for one pair. in contrast, pip964 stably coexisted wit ...	1987	3107464
the effect of transferrin and lysozyme on antibacterial activity of amniotic fluid.	amniotic fluid obtained from normal full term gestation inhibited the growth of e. coli, staph. aureus and b. subtilis, while ps. aeruginosa, str. faecalis and str. agalactiae proliferated readily in amniotic fluid. but when amniotic fluid was heated at 100 degrees c for 5 minutes, its antibacterial activity was completely lost. the levels of transferrin and lysozyme in amniotic fluid at term were determined to be 29.1 +/- 17.6 mg/100 ml (n = 90) and 19.1 +/- 8.3 micrograms/ml (n = 145), respect ...	1987	3107622
penetration in vitro of human and ferret dentine by three bacterial species in relation to their potential role in pulpal inflammation.		1986	3108162
significance of in-vitro penicillin tolerance in experimental enterococcal endocarditis.	although penicillin tolerance has been increasingly recognized among clinical isolates of many gram-positive organisms, the significance of this phenomenon in vivo is not clear. the present study was performed to characterize penicillin-tolerant enterococci by several in-vitro parameters and to examine the significance in vivo in a rabbit model of infective endocarditis. tolerant enterococci exhibited several characteristics which distinguished them from non-tolerant bacteria: significantly grea ...	1987	3108228
identification of streptococcus faecalis and streptococcus faecium and susceptibility studies with newly developed antimicrobial agents.	identification and susceptibility studies were performed on 301 blood and urine streptococcus faecalis and streptococcus faecium isolates. strep trio-tubes s4, s5, and s3 (carr-scarborough microbiologicals, inc.) were compared with conventional methods for accuracy and rapidity. of 282 isolates identified as s. faecalis, 98% were identified by species in 4 h with trio-tubes; the same percentage of isolates analyzed by conventional methods were identified in 24 h. all 14 s. faecium isolates (appr ...	1987	3108309
occult cecal perforation as cause of necrotizing infection.		1987	3108710