Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the genus desulfovibrio: the centennial. | 1995 | 16535089 | |
| acetogenesis from dichloromethane by a two-component mixed culture comprising a novel bacterium. | a strictly anaerobic two-component culture able to grow exponentially with a doubling time of 20 h on a medium containing dichloromethane as the carbon and energy source was characterized. on a medium without sulfate, we observed (per mol of dichloromethane) a mass balance of 2 mol of chloride, 0.26 mol of acetate, 0.05 mol of formate, and 0.25 mol of carbon in biomass. one component of the culture, strain dmb, was identified by a 16s ribosomal dna analysis as a desulfovibrio sp. the other compo ... | 1995 | 16535097 |
| interspecies acetate transfer influences the extent of anaerobic benzoate degradation by syntrophic consortia. | benzoate degradation by an anaerobic, syntrophic bacterium, strain sb, in coculture with desulfovibrio sp. strain g-11 reached a threshold value which depended on the amount of acetate added and ranged from about 2.5 to 29.9 (mu)m. increasing acetate concentrations also uncompetitively inhibited benzoate degradation. the apparent v(infmax) and apparent k(infm) for benzoate degradation decreased with increasing acetate concentration, but the benzoate degradation capacities (v(infmax)/k(infm)) of ... | 1996 | 16535215 |
| cometabolic transformation and cleavage of nitrodiphenylamines by three newly isolated sulfate-reducing bacterial strains. | three sulfate-reducing bacterial strains (desulfovibrio sp. strain shv, desulfococcus sp. strain whc, and desulfomicrobium sp. strain whb) with the capacity to cometabolize 2-nitrodiphenylamine, 4-nitrodiphenylamine, and 2,4-dinitrodiphenylamine were newly isolated. before breaking down the diphenylamine structure, these strains cometabolically reduce the nitrodiphenylamines to the corresponding aminodiphenylamines during anaerobic oxidation of the growth substrate lactate (desulfovibrio strain ... | 1996 | 16535317 |
| dissolution of barium from barite in sewage sludges and cultures of desulfovibrio desulfuricans. | high concentrations of total barium, ranging from 0.42 to 1.58 mg(middot)g(sup-1) (dry weight) were found in sludges of two sewage treatment plants near florence, italy. barium concentrations in the suspended matter decreased as redox potential values changed from negative to positive. an anoxic sewage sludge sample was aerated, and 30% of the total barium was removed in 24 h. to demonstrate that barium was solubilized from barite by sulfate-reducing bacteria, a strain of desulfovibrio desulfuri ... | 1996 | 16535353 |
| diversity of nitrogen fixation genes in the symbiotic intestinal microflora of the termite reticulitermes speratus. | the diversity of nitrogen-fixing organisms in the symbiotic intestinal microflora of a lower termite, reticulitermes speratus, was investigated without culturing the resident microorganisms. fragments of the nifh gene, which encodes the dinitrogenase reductase, were directly amplified from the dna of the mixed microbial population in the termite gut and were clonally isolated. the phylogenetic analysis of the nifh product amino acid sequences showed that there was a remarkable diversity of nitro ... | 1996 | 16535372 |
| competition and coexistence of sulfate-reducing and methanogenic populations in anaerobic biofilms. | the microbial population structure and function of natural anaerobic communities maintained in laboratory fixed-bed biofilm reactors were tracked before and after a major perturbation, which involved the addition of sulfate to the influent of a reactor that had previously been fed only glucose (methanogenic), while sulfate was withheld from a reactor that had been fed both glucose and sulfate (sulfidogenic). the population structure, determined by using phylogenetically based oligonucleotide pro ... | 1996 | 16535428 |
| reduction of hexavalent uranium from organic complexes by sulfate- and iron-reducing bacteria. | the influence of organic-hexavalent-uranium [u(vi)] complexation on u(vi) reduction by a sulfate-reducing bacterium (desulfovibrio desulfuricans) and an iron-reducing bacterium (shewanella alga) was evaluated. four aliphatic ligands (acetate, malonate, oxalate, and citrate) and an aromatic ligand (tiron [4,5-dihydroxy-1,3-benzene disulfonic acid]) were used to study complexed-uranium bioavailability. the trends in uranium reduction varied with the nature and the amount of u(vi)-organic complex f ... | 1997 | 16535729 |
| effect of influent cod/so4(2-) ratios on mesophilic anaerobic reactor biomass populations: physico-chemical and microbiological properties. | the competitive and syntrophic interactions between different anaerobic bacterial trophic groups in sulphate limited expanded granular sludge bed (egsb) reactors was investigated. the outcome of competition between the sulphate-reducing, methanogenic and syntrophic populations after development in reactors at varying influent cod/so4 (2-) ratios was examined in batch activity tests with the inclusion of specific sulphate reducing bacteria (srb) and methane producing archaea (mpa) inhibitors. srb ... | 2006 | 16542412 |
| kinetics studies of the superoxide-mediated electron transfer reactions between rubredoxin-type proteins and superoxide reductases. | in this work we present a kinetic study of the superoxide-mediated electron transfer reactions between rubredoxin-type proteins and members of the three different classes of superoxide reductases (sors). sors from the sulfate-reducing bacteria desulfovibrio vulgaris (dv) and d. gigas (dg) were chosen as prototypes of classes i and ii, respectively, while sor from the syphilis spirochete treponema pallidum (tp) was representative of class iii. our results show evidence for different behaviors of ... | 2006 | 16544159 |
| two-component signal transduction systems of desulfovibrio vulgaris: structural and phylogenetic analysis and deduction of putative cognate pairs. | a large number of two-component signal transduction systems (tcsts) including 59 putative sensory histidine kinases (hk) and 55 response regulators (rr) were identified from the desulfovibrio vulgaris genome. in this study, the structural and phylogenetic analyses of all putative tcstss in d. vulgaris were performed. the results showed that d. vulgaris contained 21 hybrid-type hks, implying that multiple-step phosphorelay may be a common signal transduction mechanism in d. vulgaris. despite the ... | 2006 | 16547644 |
| characterization and expression analysis of the cytochrome bd oxidase operon from desulfovibrio gigas. | although classified as anaerobic, desulfovibrio gigas contains a functional canonical membrane respiratory chain, including a cytochrome bd quinol oxidase as its terminal element. in the present study, we report the identification of the operon cydab encoding the two subunits of cytochrome bd from this bacterium. two hypothetical promoter regions and sequences resembling transcriptional regulators-binding sites have been identified. amino acid sequence analysis revealed a high similarity to cyto ... | 2006 | 16550467 |
| evolution of four gene families with patchy phylogenetic distributions: influx of genes into protist genomes. | lateral gene transfer (lgt) in eukaryotes from non-organellar sources is a controversial subject in need of further study. here we present gene distribution and phylogenetic analyses of the genes encoding the hybrid-cluster protein, a-type flavoprotein, glucosamine-6-phosphate isomerase, and alcohol dehydrogenase e. these four genes have a limited distribution among sequenced prokaryotic and eukaryotic genomes and were previously implicated in gene transfer events affecting eukaryotes. if our pr ... | 2006 | 16551352 |
| studies on thermophilic sulfate-reducing bacteria iii. : adenosine triphosphate-sulfurylase of clostridium nigrificans and desulfovibrio desulfuricans. | akagi, j. m. (university of kansas, lawrence) and l. leon campbell. studies on thermophilic sulfate-reducing bacteria. iii. adenosine triphosphate-sulfurylase of clostridium nigrificans and desulfovibrio desulfuricans. j. bacteriol. 84:1194-1201. 1962.-adenosine triphosphate (atp)-sulfurylase, which catalyzes the formation of adenosine-5'-phosphosulfate (aps) from atp and so(4) (=), has been purified from crude extracts of clostridium nigrificans and desulfovibrio desulfuricans by (nh(4))(2)so(4 ... | 1962 | 16561978 |
| chromate reduction by immobilized palladized sulfate-reducing bacteria. | resting cells of desulfovibrio vulgaris ncimb 8303 and desulfovibrio desulfuricans ncimb 8307 were used for the hydrogenase-mediated reduction of pd(ii) to pd(0). the resulting hybrid palladium bionanocatalyst (bio-pd(0)) was used in the reduction of cr(vi) to the less environmentally problematic cr(iii) species. the reduction of cr(vi) by free and agar-immobilized bio-pd(0) was evaluated. investigations using catalyst suspensions showed that cr(vi) reduction was similar ( approximately 170 nmol ... | 2006 | 16570313 |
| genetic transfer in desulfovibrio desulfuricans. | an apparently defective bacteriophage capable of mediating transduction has been identified in culture filtrates of desulfovibrio desulfuricans (american type culture collection 27774). phage-mediated intraspecies transfer of antibiotic resistance markers occurs with a frequency of 10(-5) to 10(-6) per recipient cell. the vector contains linear fragments of double-stranded dna of about 13.5 kilobase pairs, which appear to be random pieces of bacterial dna. as yet, neither induction nor plaque fo ... | 1987 | 16578824 |
| [carboanhydrase activity in halophilic anaerobes from soda lakes]. | the activity and cellular localization of carboanhydrase (ca) in two alkaliphilic anaerobes growing in soda lakes at ph 9-10 was studied. ca activity in the cell extracts of the acetogenic bacterium natroniella acetigena was comparable to that of the neutrophilic acetogens. hydrogenotrophically grown cells of desulfonatronum lacustre exhibited higher ca activity compared to the cells grown on media with formate. high ca activity in the cytoplasmic fraction and the absence of high activity in the ... | 2006 | 16579438 |
| [distribution, diversity, and activity of sulfate-reducing bacteria in the water column in gek-gel lake, azerbaijan]. | the distribution and activity of sulfate-reducing bacteria (srb) in the water column of the alpine meromictic gek-gel lake were studied. apart from traditional microbiological methods based on cultivation and on measuring the process rates with radioactive labels, in situ fluorescent hybridization (fish) was used, which enables identification and quantification without cultivating organisms. the peak rate of sulfate reduction, 0.486 microg s/(l day), was found in the chemocline at 33 m. the peak ... | 2006 | 16579451 |
| desulfovibrio desulfuricans g20 tetraheme cytochrome structure at 1.5 angstrom and cytochrome interaction with metal complexes. | the structure of the type i tetraheme cytochrome c(3) from desulfovibrio desulfuricans g20 was determined to 1.5 angstrom by x-ray crystallography. in addition to the oxidized form, the structure of the molybdate-bound form of the protein was determined from oxidized crystals soaked in sodium molybdate. only small structural shifts were obtained with metal binding, consistent with the remarkable structural stability of this protein. in vitro experiments with pure cytochrome showed that molybdate ... | 2006 | 16580681 |
| thioredoxin system in obligate anaerobe desulfovibrio desulfuricans: identification and characterization of a novel thioredoxin 2. | metal corroding sulfate reducing bacteria have been poorly characterized at molecular level due to difficulties pertaining to isolation and handling of anaerobes. we report here for the first time the presence and characterization of thioredoxin 2 in an obligate anaerobic dissimilatory sulfate reducing bacterium desulfovibrio desulfuricans. in silico analysis of the d. desulfuricans genome revealed the presence of thioredoxin 1 (dstrx1), thioredoxin 2 (dstrx2) and thioredoxin reductase (dstrxr) ... | 2006 | 16580795 |
| desulfovibrio frigidus sp. nov. and desulfovibrio ferrireducens sp. nov., psychrotolerant bacteria isolated from arctic fjord sediments (svalbard) with the ability to reduce fe(iii). | strains 18t, 61t and 77 were isolated from two permanently cold fjord sediments on the west coast of svalbard. the three psychrotolerant strains, with temperature optima at 20-23 degrees c, were able to grow at the freezing point of sea water, -2 degrees c. the strains oxidized important fermentation products such as hydrogen, formate and lactate with sulfate as the electron acceptor. sulfate could be replaced by sulfite, thiosulfate or elemental sulfur. poorly crystalline and soluble fe(iii) co ... | 2006 | 16585676 |
| desulfovibrio gigas flavodiiron protein affords protection against nitrosative stress in vivo. | desulfovibrio gigas flavodiiron protein (fdp), rubredoxin:oxygen oxidoreductase (roo), was proposed to be the terminal oxidase of a soluble electron transfer chain coupling nadh oxidation to oxygen reduction. however, several members from the fdp family, to which roo belongs, revealed nitric oxide (no) reductase activity. therefore, the protection afforded by roo against the cytotoxic effects of no was here investigated. the no and oxygen reductase activities of recombinant roo in vitro were tes ... | 2006 | 16585735 |
| the atp-dependent reduction of sulfate with hydrogen in extracts of desulfovibrio desulfuricans. | 1959 | 16590430 | |
| photosensitized production of hydrogen by hydrogenase in reversed micelles. | hydrogenase (hydrogen:ferricytochrome c(3) oxidoreductase, ec 1.12.2.1) from desulfovibrio vulgaris was encapsulated in reversed micelles with cetyltrimethylammonium bromide as surfactant and a chloroform/octane mixture as solvent. reducing equivalents for hydrogenase-catalyzed hydrogen production were provided by vectorial photosensitized electron transfer from a donor (thiophenol) in the organic phase through a surfactant-ru(2+) sensitizer located in the interphase to methyl viologen concentra ... | 1982 | 16593204 |
| hydrogen production and deuterium-proton exchange reactions catalyzed by desulfovibrio nickel(ii)-substituted rubredoxins. | the nickel tetrahedral sulfur-coordinated core formed upon metal replacement of the native iron in desulfovibrio sp. rubredoxins is shown to mimic the reactivity pattern of nickel-containing hydrogenases with respect to hydrogen production, deuterium-proton exchange, and inhibition by carbon monoxide. | 1988 | 16594005 |
| a tale of two ferredoxins: sequence similarity and structural differences. | sequence similarity between proteins is usually considered a reliable indicator of homology. pyruvate-ferredoxin oxidoreductase and quinol-fumarate reductase contain ferredoxin domains that bind [fe-s] clusters and are involved in electron transport. profile-based methods for sequence comparison, such as psi-blast and hmmer, suggest statistically significant similarity between these domains. | 2006 | 16603087 |
| relation between mrna expression and sequence information in desulfovibrio vulgaris: combinatorial contributions of upstream regulatory motifs and coding sequence features to variations in mrna abundance. | the context-dependent expression of genes is the core for biological activities, and significant attention has been given to identification of various factors contributing to gene expression at genomic scale. however, so far this type of analysis has been focused either on relation between mrna expression and non-coding sequence features such as upstream regulatory motifs or on correlation between mrna abundance and non-random features in coding sequences (e.g., codon usage and amino acid usage) ... | 2006 | 16603130 |
| changing the ligation of the distal [4fe4s] cluster in nife hydrogenase impairs inter- and intramolecular electron transfers. | in nife hydrogenases, electrons are transferred from the active site to the redox partner via a chain of three iron-sulfur clusters, and the surface-exposed [4fe4s] cluster has an unusual his(cys)3 ligation. when this histidine (h184 in desulfovibrio fructosovorans) is changed into a cysteine or a glycine, a distal cubane is still assembled but the oxidative activity of the mutants is only 1.5 and 3% of that of the wt, respectively. we compared the activities of the wt and engineered enzymes for ... | 2006 | 16608357 |
| hydrogen bonding affects the [nife] active site of desulfovibrio vulgaris miyazaki f hydrogenase: a hyperfine sublevel correlation spectroscopy and density functional theory study. | pulse electron paramagnetic resonance and hyperfine sublevel correlation spectroscopy have been used to investigate nitrogen coordination of the active site of [nife] hydrogenase of desulfovibrio vulgaris miyazaki f in its oxidized "ready" state. the obtained (14)n hyperfine (a = [+1.32, +1.32, +2.07] mhz) and nuclear quadrupole (e(2)qq/h = -1.9 mhz, eta = 0.37) coupling constants were assigned to the n(epsilon) of a highly conserved histidine (his88) by studying a hydrogenase preparation in whi ... | 2006 | 16610917 |
| the repetitive dna elements called crisprs and their associated genes: evidence of horizontal transfer among prokaryotes. | we have found direct dna repeats 21-47 bp in length interspersed with nonrepetitive sequences of similar length, or clustered regularly interspaced short palindromic repeats (crisprs) in a wide range of diverse prokaryotes, including many archaeal and eubacterial species. a number of cas, crispr-associated genes have also been characterized in many of the same organisms. phylogenetic analysis of these cas genes suggests that the crispr loci have been propagated via hgt, horizontal gene transfer. ... | 2006 | 16612537 |
| selenium is involved in regulation of periplasmic hydrogenase gene expression in desulfovibrio vulgaris hildenborough. | desulfovibrio vulgaris hildenborough is a good model organism to study hydrogen metabolism in sulfate-reducing bacteria. hydrogen is a key compound for these organisms, since it is one of their major energy sources in natural habitats and also an intermediate in the energy metabolism. the d. vulgaris hildenborough genome codes for six different hydrogenases, but only three of them, the periplasmic-facing [fefe], [feni]1, and [fenise] hydrogenases, are usually detected. in this work, we studied t ... | 2006 | 16621815 |
| desulfovibrio alkalitolerans sp. nov., a novel alkalitolerant, sulphate-reducing bacterium isolated from district heating water. | a novel alkalitolerant, sulphate-reducing bacterium (strain rt2t) was isolated from alkaline district heating water. strain rt2t was a motile vibrio (0.5-0.8 microm wide and 1.4-1.9 microm long) and grew at ph 6.9-9.9 (optimum at ph 9.0-9.4) and at 16-47 degrees c (optimum at 43 degrees c). the genomic dna g+c content was 64.7 mol%. a limited number of compounds were used as electron donors with sulphate as electron acceptor, including lactate, pyruvate, formate and hydrogen/acetate. sulphite an ... | 2006 | 16627648 |
| ascorbate as a substrate for photoproduction of hydrogen by photosystem i of chloroplasts. | the photoproduction of hydrogen by 2-(3,4-dichlorophenyl)-1,1-dimethylurea (dcmu)-inhibited chloroplasts from ascorbate under anaerobic conditions was studied in the ph range 5.0 to 7.5 using methyl viologen (mv), n,n,n',n'-tetramethyl-p-phenylenediamine (tmpd), and excess hydrogenase from desulfovibrio desulfuricans. (a) at neutral and basic phs, the photoreduction of mv, which reacted back with photoxidized ascorbate (dehydroascorbate [dhasc]), and the rates of h(2) photoproduction were very l ... | 1982 | 16662354 |
| improved methodology for bioremoval of black crusts on historical stone artworks by use of sulfate-reducing bacteria. | an improved methodology to remove black crusts from stone by using desulfovibrio vulgaris subsp. vulgaris atcc 29579, a sulfate-reducing bacterium, is presented. the strain removed 98% of the sulfates of the crust in a 45-h treatment. precipitation of black iron sulfide was avoided using filtration of a medium devoid of iron. among three cell carriers, carbogel proved to be superior to both sepiolite and hydrobiogel-97, as it allowed an easy application of the bacteria, kept the system in a stat ... | 2006 | 16672524 |
| integrated analysis of transcriptomic and proteomic data of desulfovibrio vulgaris: zero-inflated poisson regression models to predict abundance of undetected proteins. | motivation: integrated analysis of global scale transcriptomic and proteomic data can provide important insights into the metabolic mechanisms underlying complex biological systems. however, because the relationship between protein abundance and mrna expression level is complicated by many cellular and physical processes, sophisticated statistical models need to be developed to capture their relationship. results: in this study, we describe a novel data-driven statistical model to integrate whol ... | 2006 | 16675466 |
| changes in organic matter biodegradability influencing sulfate reduction in an aquifer contaminated by landfill leachate. | in situ experiments were conducted to measure sulfate reduction rates and identify rate-limiting factors in a shallow, alluvial aquifer contaminated with municipal landfill leachate. single-well, push-pull tests conducted in a well adjacent to the landfill with > 8 mm dissolved organic carbon (doc) exhibited a sulfate reduction rate of 3.2 mumol so4(-2) (l sediment)(-1) day(-1), a value in close agreement with laboratory-derived estimates. identical tests conducted in wells located 90 m downgrad ... | 2006 | 16680512 |
| oxidative stress and heat-shock responses in desulfovibrio vulgaris by genome-wide transcriptomic analysis. | sulfate-reducing bacteria such as desulfovibrio vulgaris have developed a set of responses that allow them to survive in hostile environments. to obtain further knowledge of the protective mechanisms employed by d. vulgaris in response to oxidative stress and heat shock, we performed a genome-wide transcriptomic analysis to determine the cellular responses to both stimuli. the results showed that 130 genes were responsive to oxidative stress, while 427 genes were responsive to heat-shock. functi ... | 2006 | 16680520 |
| [adhesion of sulphate-reducing bacteria to steel under cathode polarization]. | adhesion of different (as to their corrosion aggression) strains of sulphate-reducing bacteria to steel has been studied under cathode polarization at various potentials: -800, -900, -1000, -1200 mv. it has been established that cathode polarization differently affects the adhesion of strain of sulphate-reducing bacteria with various aggression to steel. correlation between the bacterial strains aggression and the number of cells adhered to metal have been noted. the cells of aggressive strains ... | 2006 | 16686219 |
| ftir-spectroscopic studies of the fine structure of nitrocellulose treated by desulfovibrio desulfuricans. | most studies have concluded that nitrocellulose (nc) with high degree of nitrogen content is resistant to biodegradation. our results demonstrated that nc (>11%n) does undergo biotransformation in the presence of sulfate-reducing bacteria desulfovibrio desulfuricans 1388. ftir analyses indicated that the substitution of nitro groups for oh(-) groups took place. the spectrum of precipitate obtained after acetone extraction of nc resembled mainly the spectrum of native cellulose. thus the syntheti ... | 2005 | 16701591 |
| carbon monoxide inhibits superoxide dismutase and stimulates reactive oxygen species production by desulfovibrio desulfuricans 1388. | the hypothesis that oxidative stress characterized by enhanced superoxide generation underlies the toxicity of some factors to living organisms has been investigated. it is shown that co (5-6% in gas phase) changed some growth parameters (mu, t(d)) of the sulfate-reducing bacterium desulfovibrio desulfuricans 1388. enhanced o(2)(-) generation registered by epr spectroscopy and adrenochrome method was observed when cells were incubated under co. the sod activity in cells from the exponential grow ... | 2005 | 16701596 |
| desulfovibrio aerotolerans sp. nov., an oxygen tolerant sulphate-reducing bacterium isolated from activated sludge. | a new mesophilic sulphate-reducing bacterium, designated strain dvo5(t) (t=type strain), was isolated from the outermost sulphate reduction-positive most-probable-number tube (10(-6) dilution) of an activated sludge sample, which had been oxygenated at 100% air saturation for 120 h. the motile, gram-negative, curved 1 by 2-5 microm and non-spore-forming cells of strain dvo5(t) existed singly or in chains. strain dvo5(t) grew optimally at 29 degrees c, ph 6.9 and 0.05% (w/v) nacl in a medium cont ... | 2005 | 16701597 |
| afm study of the effect of metronidazole on surface structures of sulfate-reducing bacteria. | the effect of metronidazole (me) on sulfate-reducing bacteria (srb) was studied by atomic force microscopy (afm) in this paper. topography images of srb cell show that after exposure to me individual cell shape is sharply modified. topography and phase images of srb cell wall show that after exposure to me not only the roughness of the cell wall increases but also the physical performance of srb surface is changed to be uniform. afm frictional loops show that after exposure to me, srb surface fr ... | 2006 | 16701623 |
| culture and identification of desulfovibrio spp. from corals infected by black band disease on dominican and florida keys reefs. | black band disease (bbd) of corals is characterized as a pathogenic microbial consortium composed of a wide variety of microorganisms. together, many of these microorganisms contribute to an active sulfur cycle that produces anoxia and high levels of sulfide adjacent to the coral surface, conditions that are lethal to coral tissue. sulfate-reducing bacteria, as sulfide producers, are an important component of the sulfur cycle and the black band community. previous molecular survey studies have s ... | 2006 | 16703774 |
| toxic effects of uranium on desulfovibrio desulfuricans g20. | the toxic effects of u(vi) were studied using desulfovibrio desulfuricans g20 in a medium containing bicarbonate or 1,4-piperazinediethane sulfonic acid disodium salt monohydrate (pipes) buffer (each at 30 mm and ph 7). uranium(vi) toxicity was dependent on the medium buffer and was observed in terms of longer lag times and, in some cases, no measurable growth. the minimum inhibiting concentration was 140 microm u(vi) in pipes-buffered medium. this is 36-fold lower than that reported previously ... | 2006 | 16704053 |
| uranium reduction. | the dramatic decrease in solubility accompanying the reduction of u(vi) to u(iv), producing the insoluble mineral uraninite, has been viewed as a potential mechanism for sequestration of environmental uranium contamination. in the past 15 years, it has been firmly established that a variety of bacteria exhibit this reductive capacity. to obtain an understanding of the microbial metal metabolism, to develop a practical approach for the acceleration of in situ bioreduction, and to predict the long ... | 2006 | 16704344 |
| salt stress in desulfovibrio vulgaris hildenborough: an integrated genomics approach. | the ability of desulfovibrio vulgaris hildenborough to reduce, and therefore contain, toxic and radioactive metal waste has made all factors that affect the physiology of this organism of great interest. increased salinity is an important and frequent fluctuation faced by d. vulgaris in its natural habitat. in liquid culture, exposure to excess salt resulted in striking elongation of d. vulgaris cells. using data from transcriptomics, proteomics, metabolite assays, phospholipid fatty acid profil ... | 2006 | 16707698 |
| global transcriptomic analysis of desulfovibrio vulgaris on different electron donors. | whole-genome microarrays of desulfovibrio vulgaris were used to determine relative transcript levels in cells grown to exponential or stationary phase on a medium containing either lactate or formate as electron donor. the results showed that 158 and 477 genes were differentially expressed when comparing exponential to stationary phase in lactate- or formate-based media, respectively; and 505 and 355 genes were responsive to the electron donor used at exponential or stationary phase, respectivel ... | 2006 | 16710634 |
| oxygen defense in sulfate-reducing bacteria. | sulfate-reducing bacteria (srb) are strict anaerobes that are often found in biotopes where oxic conditions can temporarily exist. the bacteria have developed several defense strategies in order to survive exposure to oxygen. these strategies includes peculiar behaviors in the presence of oxygen, like aggregation or aerotaxis, and enzymatic systems dedicated to the reduction and the elimination of oxygen and its reactive species. sulfate-reducing bacteria, and specially desulfovibrio species, po ... | 2006 | 16713001 |
| folding of desulfovibrio desulfuricans flavodoxin is accelerated by cofactor fly-casting. | folding of cofactor-binding proteins involves ligand binding in addition to polypeptide folding. we here assess the kinetic folding/binding landscape for desulfovibrio desulfuricans flavodoxin that coordinates an fmn cofactor. the apo-form folds in a two-step process involving a burst-phase intermediate. studies on tyr98ala and trp60ala variants reveal that these aromatics-that stack with the fmn in the holo-form-are not participating in the apo-protein folding pathway. however, these residues a ... | 2006 | 16730634 |
| pathways of h2 toward the active site of [nife]-hydrogenase. | hydrogenases catalyze the reversible oxidation of molecular hydrogen (h(2)), but little is known about the diffusion of h(2) toward the active site. here we analyze pathways for h(2) permeation using molecular dynamics (md) simulations in explicit solvent. various md simulation replicates were done, to improve the sampling of the system states. h(2) easily permeates hydrogenase in every simulation and it moves preferentially in channels. all h(2) molecules that reach the active site made their a ... | 2006 | 16731562 |
| solution structure of hndac: a thioredoxin-like domain involved in the nadp-reducing hydrogenase complex. | the nadp-reducing hydrogenase complex from desulfovibrio fructosovorans is a heterotetramer encoded by the hndabcd operon. sequence analysis indicates that the hndc subunit (52 kda) corresponds to the nadp-reducing unit, and the hndd subunit (63.5 kda) is homologous to clostridium pasteurianum hydrogenase. the role of hnda and hndb subunits (18.8 kda and 13.8 kda, respectively) in the complex remains unknown. the hnda subunit belongs to the [2fe-2s] ferredoxin family typified by c. pasteurianum ... | 2006 | 16731971 |
| low strength wastewater treatment under low temperature conditions by a novel sulfur redox action process. | the objective of this research is to make a novel wastewater treatment process activated by a sulfur-redox cycle action of microbes in low temperature conditions. this action is carried out by sulfate-reducing bacteria (srb) and sulfur-oxidizing bacteria (sob). the process was comprised of a uasb reactor as pre-treatment and an aerobic downflow hanging sponge (dhs) reactor as post-treatment. as the results of reactor operation, the whole process achieved that over 90% of codcr removal efficiency ... | 2006 | 16749445 |
| energetic consequences of nitrite stress in desulfovibrio vulgaris hildenborough, inferred from global transcriptional analysis. | many of the proteins that are candidates for bioenergetic pathways involved with sulfate respiration in desulfovibrio spp. have been studied, but complete pathways and overall cell physiology remain to be resolved for many environmentally relevant conditions. in order to understand the metabolism of these microorganisms under adverse environmental conditions for improved bioremediation efforts, desulfovibrio vulgaris hildenborough was used as a model organism to study stress response to nitrite, ... | 2006 | 16751553 |
| a chemotaxis operon in the bacterium desulfovibrio gigas is induced under several growth conditions. | the chemosensory system of bacteria controls their motility and behaviour in different environments. in the present study, we report the identification of the first chemotaxis operon in desulfovibrio gigas. amino acid sequence analysis revealed seven coding regions for polypeptides with a high similarity to chemotaxis proteins from other organisms. d. gigas chemotaxis operon has a similar genetic organisation to chemotaxis operons found in the sequenced genomes of desulfovibrio desulfuricans and ... | 2006 | 16753818 |
| crystallization and preliminary structure determination of the membrane-bound complex cytochrome c nitrite reductase from desulfovibrio vulgaris hildenborough. | the cytochrome c nitrite reductase (cnir) isolated from desulfovibrio vulgaris hildenborough is a membrane-bound complex formed of nrfa and nrfh subunits. the catalytic subunit nrfa is a soluble pentahaem cytochrome c that forms a physiological dimer of about 120 kda. the electron-donor subunit nrfh is a membrane-anchored tetrahaem cytochrome c of about 18 kda molecular weight and belongs to the napc/nirt family of quinol dehydrogenases, for which no structures are known. crystals of the native ... | 2006 | 16754983 |
| rapid and reversible reactions of [nife]-hydrogenases with sulfide. | rapid and reversible binding of sulfide to [nife]-hydrogenases (particularly the enzyme from desulfovibrio vulgaris) under weakly acidic conditions (ph 6) has been studied by protein film voltammetry, which tracks the formation of different species as a function of potential. sulfide (most likely entering as h2s) rapidly attacks the active site during h2 oxidation. the inactive adduct is formed (and is stable) only at potentials substantially more positive than the comparable species formed with ... | 2006 | 16756292 |
| characterization of bacterial community associated to biofilms of corroded oil pipelines from the southeast of mexico. | microbial communities associated to biofilms promote corrosion of oil pipelines. the community structure of bacteria in the biofilm formed in oil pipelines is the basic knowledge to understand the complexity and mechanisms of metal corrosion. to assess bacterial diversity, biofilm samples were obtained from x52 steel coupons corroded after 40 days of exposure to normal operation and flow conditions. the biofilm samples were directly used to extract metagenomic dna, which was used as template to ... | 2006 | 16765858 |
| study of nitrate stress in desulfovibrio vulgaris hildenborough using itraq proteomics. | the response of desulfovibrio vulgaris hildenborough (dvh), a sulphate-reducing bacterium, to nitrate stress was examined using quantitative proteomic analysis. dvh was stressed with 105 mm sodium nitrate (nano(3)), a level that caused a 50% inhibition in growth. the protein profile of stressed cells was compared with that of cells grown in the absence of nitrate using the itraq peptide labelling strategy and tandem liquid chromatography separation coupled with mass spectrometry (quadrupole time ... | 2006 | 16772278 |
| a humanized gnotobiotic mouse model of host-archaeal-bacterial mutualism. | our colons harbor trillions of microbes including a prominent archaeon, methanobrevibacter smithii. to examine the contributions of archaea to digestive health, we colonized germ-free mice with bacteroides thetaiotaomicron, an adaptive bacterial forager of the polysaccharides that we consume, with or without m. smithii or the sulfate-reducing bacterium desulfovibrio piger. whole-genome transcriptional profiling of b. thetaiotaomicron, combined with mass spectrometry, revealed that, unlike d. pig ... | 2006 | 16782812 |
| epr and redox properties of periplasmic nitrate reductase from desulfovibrio desulfuricans atcc 27774. | nitrate reductases are enzymes that catalyze the conversion of nitrate to nitrite. we report here electron paramagnetic resonance (epr) studies in the periplasmic nitrate reductase isolated from the sulfate-reducing bacteria desulfovibrio desulfuricans atcc 27774. this protein, belonging to the dimethyl sulfoxide reductase family of mononuclear mo-containing enzymes, comprises a single 80-kda subunit and contains a mo bis(molybdopterin guanosine dinucleotide) cofactor and a [4fe-4s] cluster. epr ... | 2006 | 16791644 |
| biosynthesis of hopanoids by sulfate-reducing bacteria (genus desulfovibrio). | sulfate reduction accounts for about a half of the remineralization of organic carbon in anoxic marine shelf regions. moreover, it was already a major microbial process in the very early ocean at least 2.4 billion years before the present. here we demonstrate for the first time the capability of sulfate-reducing bacteria (srb) to biosynthesize hopanoids, compounds that are quantitatively important and widely distributed biomarkers in recent and fossil sediments dating back to the late archean. w ... | 2006 | 16817930 |
| a proteomic view of desulfovibrio vulgaris metabolism as determined by liquid chromatography coupled with tandem mass spectrometry. | direct lc-ms/ms was used to examine the proteins extracted from exponential or stationary phase desulfovibrio vulgaris cells that had been grown on a minimal medium containing either lactate or formate as the primary carbon source. across all four growth conditions, 976 gene products were identified with high confidence, which is equal to approximately 28% of all predicted proteins in the d. vulgaris genome. bioinformatic analysis showed that the proteins identified were distributed among almost ... | 2006 | 16819729 |
| reclassification of the sulfate- and nitrate-reducing bacterium desulfovibrio vulgaris subsp. oxamicus as desulfovibrio oxamicus sp. nov., comb. nov. | desulfovibrio vulgaris subsp. oxamicus (type strain, dsm 1925(t)) was found to use nitrate as a terminal electron acceptor, the latter being reduced to ammonium. phylogenetic studies indicated that strain dsm 1925(t) was distantly related to the type strain of desulfovibrio vulgaris (95.4 % similarity of the small-subunit rrna gene) and had as its closest phylogenetic relatives two other nitrate- and sulfate-reducing bacteria, namely desulfovibrio termitidis (99.4 % similarity) and desulfovibrio ... | 2006 | 16825618 |
| carriage, quantification, and predominance of methanogens and sulfate-reducing bacteria in faecal samples. | to determine carriage rates and densities of methanogens and sulfate-reducing bacteria in adults and children using molecular methods, and to also determine if a reciprocal relationship exists between these organisms. | 2006 | 16834722 |
| photoinduced hydrogen production by direct electron transfer from photosystem i cross-linked with cytochrome c3 to [nife]-hydrogenase. | the photosynthetic reaction center is an efficient molecular device for the conversion of light energy to chemical energy. in a previous study, we synthesized the hydrogenase/photosystem i (psi) complex, in which ralstonia hydrogenase was linked to the cytoplasmic side of synechocystis psi, to modify psi so that it photoproduced molecular hydrogen (h2). in that study, hydrogenase was fused with a psi subunit, psae, and the resulting hydrogenase-psae fusion protein was self-assembled with psae-fr ... | 2006 | 16836469 |
| the adaptive genome of desulfovibrio vulgaris hildenborough. | peculiar attributes revealed by sequencing the genome of desulfovibrio vulgaris hildenborough are analyzed, particularly in relation to the presence of a phosphotransferase system (pts). the pts is a typical bacterial carbohydrate transport system functioning via group translocation. novel avenues for investigations are proposed emphasizing the metabolic diversity of d. vulgaris hildenborough, especially the likely utilization of mannose-type sugars. comparative analysis with pts from other gram ... | 2006 | 16842335 |
| succinate dehydrogenase functioning by a reverse redox loop mechanism and fumarate reductase in sulphate-reducing bacteria. | sulphate- or sulphur-reducing bacteria with known or draft genome sequences (desulfovibrio vulgaris, desulfovibrio desulfuricans g20, desulfobacterium autotrophicum [draft], desulfotalea psychrophila and geobacter sulfurreducens) all contain sdhcab or frdcab gene clusters encoding succinate : quinone oxidoreductases. frdd or sdhd genes are missing. the presence and function of succinate dehydrogenase versus fumarate reductase was studied. desulfovibrio desulfuricans (strain essex 6) grew by fuma ... | 2006 | 16849807 |
| [detection of srps in injection water of shenli oil field by fish]. | fluorescence in situ hybridization (fish) with 16s rrna-targeted oligonucleotide probes was applied for analyzing the structure of sulfate reducing prokaryotes (srps) community in injection water of shengli oil field. eight probes and their various combinations were used to detect srps in the water. results showed srps detected in the water were diverse, which followed in 4 bacterial phyla and 1 archaeal phylum. total amount of srps was 2.86 x 10(4) cells/ml, accounting for 20% of total cells of ... | 2006 | 16850843 |
| chemical activity of iron in [2fe-2s]-protein centers and fes2(100) surfaces. | iron atoms bonded to sulfur play an important role in proteins, heterogeneous catalysts, and gas sensors. first-principles density functional calculations were used to investigate the structure and chemical activity of a unique [2fe-2s] center in the split-soret cytochrome c (ssc) from desulfovibrio desulfuricans. in agreement with a previously proposed structural model [abreu et al., j. biol. inorg. chem. 2003, 8, 360], it is found that the [2fe-2s] cluster is located in a surface pocket of the ... | 2005 | 16851284 |
| structural basis for o2 sensing by the hemerythrin-like domain of a bacterial chemotaxis protein: substrate tunnel and fluxional n terminus. | the methyl-accepting chemotaxis protein, dcrh, from the anaerobic sulfate-reducing bacterium, desulfovibrio vulgaris (hildenborough), has a hemerythrin-like domain, dcrh-hr, at its c terminus. dcrh-hr was previously shown to contain a diiron site that binds o2, suggesting an o2-sensing function. x-ray crystal structures of diferric (met-), azido-diferric (azidomet-), and diferrous (deoxy-) dcrh-hr reveal a "substrate tunnel" distinct from that in invertebrate hemerythrins. this tunnel is propose ... | 2006 | 16866347 |
| superoxide reduction mechanism of archaeoglobus fulgidus one-iron superoxide reductase. | superoxide reductases (sors), iron-centered enzymes responsible for reducing superoxide (o2(-)) to hydrogen peroxide, are found in many anaerobic and microaerophilic prokaryotes. the rapid reaction with an exogenous electron donor renders the reductase activity catalytic. here, we demonstrate using pulse radiolysis that the initial reaction between o2(-) and archaeoglobus fulgidus neelaredoxin, a one-iron sor, leads to a short-lived transient that immediately disappears to yield a solvent-bound ... | 2006 | 16866373 |
| [microbial community structure in different wastewater treatment processes characterized by single-strand-conformation polymorphism (sscp) technique]. | in order to investigate microbial community structures in different wastewater treatment processes and understand the relationship between the structures and the status of processes, the microbial community diversity, variety and distribution in five wastewater treatment processes were studied by a culture-independent genetic fingerprinting technique single-strand-conformation polymorphism (sscp). the five processes included a denitrification and phosphorus removal bioreactor (n), chinese tradit ... | 2006 | 16881324 |
| temporal transcriptomic analysis as desulfovibrio vulgaris hildenborough transitions into stationary phase during electron donor depletion. | desulfovibrio vulgaris was cultivated in a defined medium, and biomass was sampled for approximately 70 h to characterize the shifts in gene expression as cells transitioned from the exponential to the stationary phase during electron donor depletion. in addition to temporal transcriptomics, total protein, carbohydrate, lactate, acetate, and sulfate levels were measured. the microarray data were examined for statistically significant expression changes, hierarchical cluster analysis, and promote ... | 2006 | 16885312 |
| isolation and preliminary characterization of a soluble nitrate reductase from the sulfate reducing organism desulfovibrio desulfuricans atcc 27774. | desulfovibrio desulfuricans atcc 27774 is a sulfate reducer that can adapt to nitrate respiration, inducing the enzymes required to utilize this alternative metabolic pathway. nitrite reductase from this organism has been previously isolated and characterized, but no information was available on the enzyme involved in the reduction of nitrate. this is the first report of purification to homogeneity of a nitrate reductase from a sulfate reducing organism, thus completing the enzymatic system requ ... | 1995 | 16887508 |
| formate dehydrogenase activity in cells and outer membrane blebs of desulfovibrio gigas. | formate dehydrogenase in desulfovibrio gigas was measured by following the release of 14co2 from radiolabeled formate. experiments with whole cells using sulfate as the electron acceptor revealed optimal formate dehydrogenase activity at ph 7.0 and formate utilization followed saturation kinetics. while formate dehydrogenase was constitutively produced in pyruvate or lactate media, the formate dehydrogenase activity was markedly increased in cells grown with formate as the electron donor. in cel ... | 1995 | 16887525 |
| malate metabolism by desulfovibrio gigas and its link to sulfate and fumarate reduction: purification of the malic enzyme and detection of nad(p)+ transhydrogenase activity. | malate metabolism was investigated in lactate grown cells of desulfovibrio gigas ; 3 mol of malate are converted into 2 mol succinate and 1 mol acetate. the malic enzyme (l-malate:nadp+ oxidoreductase) was purified to homogeneity and partially characterized. the enzyme is monomeric with molecular weight of 45 kda. its spectrum has no visible absorption and the activity is stimulated by k+ and mg2+. the presence of an nad(p)+ transhydrogenase, the observation of partial reduction of adenylylsulfa ... | 1995 | 16887531 |
| localization of cytochromes in the outer membrane of desulfovibrio vulgaris (hildenborough) and their role in anaerobic biocorrosion. | a protocol was developed whereby the outer and cytoplasmic membranes of the sulfate-reducing bacterium desulfovibrio vulgaris (hildenborough) were isolated and partially characterized. the isolated outer membrane fractions from cultures grown under high (100 ppm) and low (5 ppm) fe2+ conditions were compared by sds-page electrophoresis, and showed that several protein bands were derepressed under the low iron conditions, most notably at 50 kda, and 77.5 kda. outer membrane isolated from low iron ... | 1995 | 16887547 |
| bilophila wadsworthia: a unique gram-negative anaerobic rod. | although comprising less than 0.01% of the normal human gastrointestinal microbiota, bilophila wadsworthia is the third most common anaerobe recovered from clinical material obtained from patients with perforated and gangrenous appendicitis. since its discovery in 1988, b. wadsworthia has been recovered from clinical specimens associated with a variety of infections, including sepsis, liver abscesses, cholecystitis, fournier's gangrene, soft tissue abscesses, empyema, osteomyelitis, bartholiniti ... | 1997 | 16887567 |
| membrane-bound cytochromes in a sulfate-reducing strict anaerobe desulfovibrio vulgaris miyazaki f. | cytoplasmic membranes were isolated from the cells of a sulfate-reducing strict anaerobe desulfovibrio vulgaris miyazaki f and membrane-bound cytochromes were characterized. redox difference spectra at 77 k revealed the presence of cytochromes with the alpha peaks at 552 and 556 nm while co-binding difference spectra showed the presence of o-type cytochrome(s). partial purification of the cytochromes demonstrated that the membranes contain cytochromes c550, c551, c556 and possibly d1 besides hig ... | 1997 | 16887609 |
| molecular study and partial characterization of iron-only hydrogenase in desulfovibrio fructosovorans. | an iron-only hydrogenase was partially purified and characterized from desulfovibrio fructosovorans wild-type strain. the enzyme exhibits a molecular mass of 56 kda and is composed of two distinct subunits hyda and hydb (46 and 13 kda, respectively). the n-terminal amino acid sequences of the two subunits of the enzyme were determined with the aim of designing degenerate oligonucleotides. direct and inverse polymerase chain reaction techniques were used to clone the hydrogenase encoding genes. a ... | 1998 | 16887623 |
| isolation and characterisation of a novel sulphate-reducing bacterium of the desulfovibrio genus. | a novel sulphate-reducing bacterium (ind 1) was isolated from a biofilm removed from a severely corroded carbon steel structure in a marine environment. light microscopy observations revealed that cells were gram-negative, rod shaped and very motile. partial 16s rrna gene sequencing and analysis of the fatty acid profile demonstrated a strong similarity between the new species and members from the desulfovibrio genus. this was confirmed by the results obtained following purification and characte ... | 1998 | 16887631 |
| biotechnological potential of sulfate-reducing bacteria for transformation of nitrocellulose. | the biotransformation of nc by desulfovibrio sp. was studied. the mass of nc was decreased by 4.9-9.3%. the rate of nc transformation was between 46 and 73 mg nc per mg of bacterial protein in 10 days. moreover, n content (%n) in the remaining nc was reduced by 2-12%. the inhibitory effect of nc was clearly expressed when the growth of d. desulfuricans 1388 in lactate/sulfate medium was initiated. the growth rate of bacteria was 1.5-fold greater when nc was not added (0.074 and 0.05 h(-1) respec ... | 2002 | 16887675 |
| hydrogen metabolism in desulfovibrio desulfuricans strain new jersey (ncimb 8313)--comparative study with d. vulgaris and d. gigas species. | this article aims to study hydrogen production/consumption in desulfovibrio (d.) desulfuricans strain new jersey, a sulfate reducer isolated from a medium undergoing active biocorrosion and to compare its hydrogen metabolism with two other desulfovibrio species, d. gigas and d. vulgaris hildenborough. hydrogen production was followed during the growth of these three bacterial species under different growth conditions: no limitation of sulfate and lactate, sulfate limitation, lactate limitation, ... | 2002 | 16887677 |
| anti-oxidant defense of the cell desulfovibrio desulfuricans b-1388. | the extracts of desulfovibrio desulfuricans b-1388 cells, grown in anaerobic condition, display the superoxide dismutase activity. the maximum value of level activity (1.02 e/min/mg) is observed in the stationary phase of growth. essentially the whole enzyme is localized in periplasmic fraction. cells desulfovibrio desulfuricans b-1388 do not show the catalase activity but contain active nadh- and nadph-peroxidases. the activity of involved peroxidases depends on the physiological condition of c ... | 2003 | 16887686 |
| desulfovibrio capillatus sp. nov., a novel sulfate-reducing bacterium isolated from an oil field separator located in the gulf of mexico. | a new spirilloid sulfate-reducing bacterium designated strain met2(t) (t=type strain), was isolated from a mexican oil field separator. electron microscopy revealed a gram-negative cell wall consisting of a 150nm thick undulating outer membrane. strain met2(t) appeared singly or in long chains and was actively motile with a corkscrew-like motion. the isolate grew optimally at 40 degrees c, ph 7.4 and 3% nacl in a medium containing lactate, thiosulfate and yeast extract. sulfate, sulfite, thiosul ... | 2003 | 16887695 |
| spectroelectrochemical characterization of the [nife] hydrogenase of desulfovibrio vulgaris miyazaki f. | the active site in the [nife] hydrogenase of desulfovibrio vulgaris miyazaki f has been investigated by fourier transform infrared (ftir) spectroscopy. analysis of the spectra allowed the three diatomic inorganic ligands to fe in this enzyme to be identified as one co molecule and two cn(-) molecules. furthermore, ph-dependent redox titrations were performed to determine the midpoint potentials as well as the pk value of the respective reactions and revealed that each single-electron redox trans ... | 2006 | 16893172 |
| cr(vi) detoxification by desulfovibrio vulgaris strain hildenborough: microbe-metal interactions studies. | toxic heavy metals constitute a worldwide environmental pollution problem. bioremediation technologies represent efficient alternatives to the classic cleaning-up of contaminated soil and ground water. most toxic heavy metals such as chromium are less soluble and toxic when reduced than when oxidized. sulfate-reducing bacteria (srb) are able to reduce heavy metals by a chemical reduction via the production of h2s and by a direct enzymatic process involving hydrogenases and c3 cytochromes. we hav ... | 2006 | 16896506 |
| sequential and structural analysis of [nife]-hydrogenase-maturation proteins from desulfovibrio vulgaris miyazaki f. | the complete primary structure of the hyn-region in the genome of desulfovibrio vulgaris miyazaki f (dvmf), encoding the [nife]-hydrogenase and two maturation proteins has been identified. besides the formerly reported genes for the large and small subunits, this region comprises genes encoding an endopeptidase (hync) and a putative chaperone (hynd). the complete genomic region covers 4086 nucleotides including the previously published upstream located promoter region and the sequences of the st ... | 2006 | 16902753 |
| palladium and gold removal and recovery from precious metal solutions and electronic scrap leachates by desulfovibrio desulfuricans. | biomass of desulfovibrio desulfuricans was used to recover au(iii) as au(0) from test solutions and from waste electronic scrap leachate. au(0) was precipitated extracellularly by a different mechanism from the biodeposition of pd(0). the presence of cu(2+) ( approximately 2000 mg/l) in the leachate inhibited the hydrogenase-mediated removal of pd(ii) but pre-palladisation of the cells in the absence of added cu(2+) facilitated removal of pd(ii) from the leachate and more than 95% of the pd(ii) ... | 2006 | 16909331 |
| a variant system i for cytochrome c biogenesis in archaea and some bacteria has a novel ccme and no ccmh. | c-type cytochromes are characterized by post-translational covalent attachment of heme to thiols that occur in a cys-xxx-xxx-cys-his motif. three distinct biogenesis systems are known for this heme attachment. archaea are now shown to contain a significantly modified form of cytochrome c maturation system i (the ccm system). the most notable adaptation relative to the well-studied apparatus from proteobacteria and plants is a novel form of the heme chaperone ccme, lacking the highly conserved hi ... | 2006 | 16920107 |
| the tmc complex from desulfovibrio vulgaris hildenborough is involved in transmembrane electron transfer from periplasmic hydrogen oxidation. | three membrane-bound redox complexes have been reported in desulfovibrio spp., whose genes are not found in the genomes of other sulfate reducers such as desulfotalea psycrophila and archaeoglobus fulgidus. these complexes contain a periplasmic cytochrome c subunit of the cytochrome c(3) family, and their presence in these organisms probably correlates with the presence of a pool of periplasmic cytochromes c(3), also absent in the two other sulfate reducers. in this work we report the isolation ... | 2006 | 16922512 |
| rubredoxin:oxygen oxidoreductase enhances survival of desulfovibrio vulgaris hildenborough under microaerophilic conditions. | genes for superoxide reductase (sor), rubredoxin (rub), and rubredoxin:oxygen oxidoreductase (roo) are located in close proximity in the chromosome of desulfovibrio vulgaris hildenborough. protein blots confirmed the absence of roo from roo mutant and sor-rub-roo (srr) mutant cells and its presence in sor mutant and wild-type cells grown under anaerobic conditions. oxygen reduction rates of the roo and srr mutants were 20 to 40% lower than those of the wild type and the sor mutant, indicating th ... | 2006 | 16923892 |
| the role of the hybrid cluster protein in oxidative stress defense. | hybrid cluster proteins (hcp) contain two types of fe/s clusters, namely a [4fe-4s](2+/1+) or [2fe-2s](2+/1+) cluster and a novel type of hybrid cluster, [4fe-2s-2o], in the as-isolated state. although first isolated from anaerobic sulfate-reducing bacteria, the analysis of the genomic sequences reveals that genes encoding putative hybrid cluster proteins are present in a wide range of organisms, aerobic, anaerobic, or facultative, from the bacteria, archaea, and eukarya domains. despite a detai ... | 2006 | 16928682 |
| crystal structure of rubredoxin from desulfovibrio gigas to ultra-high 0.68 a resolution. | rubredoxin (d.g. rd) is a small non-heme iron-sulfur protein shown to function as a redox coupling protein from the sulfate reducing bacteria desulfovibrio gigas. the protein is generally purified from anaerobic bacteria in which it is thought to be involved in electron transfer or exchange processes. rd transfers an electron to oxygen to form water as part of a unique electron transfer chain, composed by nadh:rubredoxin oxidoreductase (nro), rubredoxin and rubredoxin:oxygen oxidoreductase (roo) ... | 2006 | 16930541 |
| proton thrusters: overview of the structural and functional features of soluble tetrahaem cytochromes c3. | tetrahaem cytochromes c (3) from sulfate-reducing bacteria have revealed exquisite complexity in their ligand binding properties and they couple the cooperative binding of two electrons with the binding of protons. in this review, the molecular mechanisms for these cooperative effects are described, and the functional consequences of these cooperativities are discussed in the context of the general mechanisms of biological energy transduction and the specific physiological metabolism of desulfov ... | 2007 | 16964504 |
| lc-ms/ms based proteomic analysis and functional inference of hypothetical proteins in desulfovibrio vulgaris. | high efficiency capillary liquid chromatography-tandem mass spectrometry (lc-ms/ms) was used to examine the proteins extracted from desulfovibrio vulgaris cells across six treatment conditions. while our previous study provided a proteomic overview of the cellular metabolism based on proteins with known functions [w. zhang, m.a. gritsenko, r.j. moore, d.e. culley, l. nie, k. petritis, e.f. strittmatter, d.g. camp ii, r.d. smith, f.j. brockman, a proteomic view of the metabolism in desulfovibrio ... | 2006 | 16982031 |
| human inflammatory bowel disease does not associate with lawsonia intracellularis infection. | there is increasing evidence that bacterial infection of the intestinal mucosa may contribute to the pathogenesis of inflammatory bowel diseases (ibd). in pigs, an obligate intracellular bacterium, lawsonia intracellularis (li), was shown to cause proliferative enteropathy (pe) of which some forms display histological and clinical similarities to human ibd. since li-similar desulfovibrio spp. may infect human cells, we hypothesized that li might be associated with the development of human ibd. | 2006 | 16984651 |
| the effect of decreasing alkalinity on microbial community dynamics in a sulfate-reducing bioreactor as analyzed by pcr-sscp. | pcr-single-strand conformation polymorphism (sscp) and southern blotting techniques were adopted to investigate microbial community dynamics in a sulfate-reducing bioreactor caused by decreasing influent alkalinity. experimental results indicated that the sulfate-removal rate approached 87% in 25 d under the conditions of influent alkalinity of 4000 mg/l (as caco3) and sulfate-loading rate of 4.8 g/(l*d), which indicated that the bioreactor started up successfully. the analysis of microbial comm ... | 2006 | 16989283 |
| synergistic inhibition of microbial sulfide production by combinations of the metabolic inhibitor nitrite and biocides. | mics of six broad-spectrum biocides and two specific metabolic inhibitors and fractional inhibitory concentration indexes (ficis) for controlling a sulfide-producing consortium were determined. nitrite was synergistic (fici<1) with all but one biocide due to its specific inhibition of dissimilatory sulfite reductase. hence, combining nitrite with biocides allows more efficient and cost-effective control of sulfate-reducing bacteria. | 2006 | 16997976 |
| recovery of temperate desulfovibrio vulgaris bacteriophage using a novel host strain. | a novel sulfate-reducing bacterium (strain depue) closely related to desulfovibrio vulgaris ssp. vulgaris strain hildenborough was isolated from the sediment of a heavy-metal impacted lake using established techniques. although few physiological differences between strains depue and hildenborough were observed, pulse-field gel electrophoresis (pfge) revealed a significant genome reduction in strain depue. comparative whole-genome microarray and polymerase chain reaction analyses demonstrated tha ... | 2006 | 17014494 |