Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| evaluation of the duopath legionella lateral flow assay for identification of legionella pneumophila and legionella species culture isolates. | duopath legionella (merck kgaa, darmstadt, germany) is a new immunochromatographic assay for the simultaneous identification of cultured l. pneumophila and legionella species other than l. pneumophila. in tests of 89 l. pneumophila strains and 87 legionella strains other than l. pneumophila representing 41 different species, duopath and a widely used latex agglutination assay detected l. pneumophila with 100% and 98% accuracy, respectively, whereas the percentages differed significantly for othe ... | 2006 | 16751575 |
| [transference of extended-spectrum beta-lactamases from nosocomial strains of klebsiella pneumoniae to other species of enterobacteriaceae]. | klebsiella pneumoniae is an important pathogenic bacterium, frequently isolated from nosocomial samples, that exhibits wide antimicrobial resistance profiles, including third generation cephalosporins (3gc), aminoglycosides and quinolones. the resistance to 3gc is mainly due to the synthesis of extended spectrum beta lactamases (esbl), encoded by conjugative plasmids. | 2006 | 16758075 |
| iscr elements: novel gene-capturing systems of the 21st century? | "common regions" (crs), such as orf513, are being increasingly linked to mega-antibiotic-resistant regions. while their overall nucleotide sequences show little identity to other mobile elements, amino acid alignments indicate that they possess the key motifs of is91-like elements, which have been linked to the mobility ent plasmids in pathogenic escherichia coli. further inspection reveals that they possess an is91-like origin of replication and termination sites (teris), and therefore crs prob ... | 2006 | 16760305 |
| structures of apo- and holo-tyrosine phenol-lyase reveal a catalytically critical closed conformation and suggest a mechanism for activation by k+ ions. | tyrosine phenol-lyase, a tetrameric pyridoxal 5'-phosphate dependent enzyme, catalyzes the reversible hydrolytic cleavage of l-tyrosine to phenol and ammonium pyruvate. here we describe the crystal structure of the citrobacter freundii holoenzyme at 1.9 a resolution. the structure reveals a network of protein interactions with the cofactor, pyridoxal 5'-phosphate, and details of coordination of the catalytically important k+ ion. we also present the structure of the apoenzyme at 1.85 a resolutio ... | 2006 | 16768450 |
| alkaline lipase production by citrobacter freundii iit-bt l139. | around 150 lipase producing bacterial isolates were screened from the local soils enriched with oil. citrobacrer freundii iit-bt l139, an isolated microbial strain, produced lipase that had high activity (8.8 u/ml) at ph 9.0 and 40 degrees c. the 16s rdna phylogenetic studies showed that citrobacter freundii belongs to the family enterobacteriaceae and later confirmed by the microbial identification. suitable c and n sources for lipase production were deduced to be starch and peptone-urea, respe ... | 2006 | 16784120 |
| exploitation of the endocytic pathway by orientia tsutsugamushi in nonprofessional phagocytes. | orientia tsutsugamushi, a causative agent of scrub typhus, is an obligate intracellular bacterium that requires the exploitation of the endocytic pathway in the host cell. we observed the localization of o. tsutsugamushi with clathrin or adaptor protein 2 within 30 min after the infection of nonprofessional phagocytes. we have further confirmed that the infectivity of o. tsutsugamushi is significantly reduced by drugs that block clathrin-mediated endocytosis but not by filipin iii, an inhibitor ... | 2006 | 16790799 |
| aspartic acid 214 in citrobacter freundii tyrosine phenol-lyase ensures sufficient c--h-acidity of the external aldimine intermediate and proper orientation of the cofactor at the active site. | in the x-ray structure of tyrosine phenol-lyase (tpl) asp214 is located at h-bonding distance from the n1 atom of the cofactor. this residue has been replaced with ala and asn and the properties of the mutant enzymes have been studied. the substitutions result in a decrease in the cofactor affinity of about four orders of magnitude. d214a and d214n tpls do not catalyze the decomposition of l-tyr and 3-fluoro-l-tyr. they decompose substrates, containing better leaving groups with rates reduced by ... | 2006 | 16793353 |
| adhesive properties of enterobacter sakazakii to human epithelial and brain microvascular endothelial cells. | enterobacter sakazakii is an opportunistic pathogen that has been associated with sporadic cases and outbreaks causing meningitis, necrotizing enterocolitis and sepsis especially in neonates. however, up to now little is known about the mechanisms of pathogenicity in e. sakazakii. a necessary state in the successful colonization, establishment and ultimately production of disease by microbial pathogens is the ability to adhere to host surfaces such as mucous membranes, gastric and intestinal epi ... | 2006 | 16800879 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2003 and march 2004]. | tendency of isolated bacteria from infections in general surgery during the period from april 2004 to march 2005 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 645 strains including 17 strains of candida spp. were isolated from 226 (79.0%) of 286 patients with surgical infections. three hundred and seventeen strains were isolated from primary infections, and 345 strains were isolated from postoperative infections. from primary infectio ... | 2006 | 16805318 |
| citrobacter infections in a tertiary care hospital in northern india. | this prospective study was carried out to look for the frequency of isolation of citrobacter species from clinical specimens and study their antimicrobial susceptibility pattern. | 2007 | 16815552 |
| discrete steps in sensing of beta-lactam antibiotics by the blar1 protein of the methicillin-resistant staphylococcus aureus bacterium. | chemical sensing by cell-surface receptors to effect signal transduction is a ubiquitous biological event. despite extensive structural biochemical study, detailed knowledge of how signal transduction occurs is largely lacking. we report herein a kinetic and structural study, obtained by stopped-flow ir spectroscopy, of the activation of the blar1 receptor of the staphylococcus aureus bacterium by beta-lactam antibiotics. the cell-surface blar1 receptor alerts the bacterium to the presence of be ... | 2006 | 16815972 |
| prospective evaluation of the risk of serious bacterial infection in children who present to the emergency department with hyperpyrexia (temperature of 106 degrees f or higher). | previous studies of children with temperatures > or = 106 degrees f (hyperpyrexia) disagree as to whether hyperpyrexia confers a high risk of serious bacterial infection. | 2006 | 16818546 |
| facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing. | sequencing of the large (>50 kb), low-copy-number (<5 per cell) plasmids that mediate horizontal gene transfer has been hindered by the difficulty and expense of isolating dna from individual plasmids of this class. we report here that a kit method previously devised for purification of bacterial artificial chromosomes (bacs) can be adapted for effective preparation of individual plasmids up to 220 kb from wild gram-negative and gram-positive bacteria. individual plasmid dna recovered from less ... | 2006 | 16820486 |
| antibiotic coresistance in extended-spectrum-beta-lactamase-producing enterobacteriaceae and in vitro activity of tigecycline. | the spread of extended-spectrum-beta-lactamase (esbl)-producing organisms, particularly those harboring the ctx-m-type enzymes, both in the hospital and in the community, is difficult to discontinue due to the successful mobilization and evolution of the genetic elements harboring esbl genes and coresistance rates in these isolates. the activities of tigecycline against 285 non-clonally related isolates (172 from escherichia coli, 84 from klebsiella spp., 20 from enterobacter spp., 5 from salmon ... | 2006 | 16870760 |
| aminoacrylate intermediates in the reaction of citrobacter freundii tyrosine phenol-lyase. | tyrosine phenol-lyase (tpl) from citrobacter freundii is a pyridoxal 5'-phosphate (plp)-dependent enzyme that catalyzes the reversible hydrolytic cleavage of l-tyr to give phenol and ammonium pyruvate. the proposed reaction mechanism for tpl involves formation of an external aldimine of the substrate, followed by deprotonation of the alpha-carbon to give a quinonoid intermediate. elimination of phenol then has been proposed to give an alpha-aminoacrylate schiff base, which releases iminopyruvate ... | 2006 | 16878992 |
| activity of rhodobacter sphaeroides rpohii, a second member of the heat shock sigma factor family. | we have identified a second rpoh homolog, rpoh(ii), in the alpha-proteobacterium rhodobacter sphaeroides. primary amino acid sequence comparisons demonstrate that r. sphaeroides rpoh(ii) belongs to a phylogenetically distinct group with rpoh orthologs from alpha-proteobacteria that contain two rpoh genes. like its previously identified paralog, rpoh(i), rpoh(ii) is able to complement the temperature-sensitive phenotype of an escherichia coli sigma(32) (rpoh) mutant. in addition, we show that rec ... | 2006 | 16885439 |
| co-production of 16s rrna methylases and extended-spectrum beta-lactamases in ampc-producing enterobacter cloacae, citrobacter freundii and serratia marcescens in korea. | 2006 | 16891325 | |
| widely distributed and predominant ctx-m extended-spectrum beta-lactamases in amsterdam, the netherlands. | three hundred sixty enterobacteriaceae and nonfermenting gram-negative bacilli, isolated during one week in may 2004 at five hospitals in amsterdam, the netherlands, were evaluated for the presence of extended-spectrum beta-lactamases (esbls). a prevalence of 7.8% was found, in contrast to the 1% observed in 1997. ctx-m esbls dominated, and four types were identified in 18 isolates. | 2006 | 16891530 |
| impact of positive preoperative surveillance blood cultures from chronic indwelling catheters in cadaveric intestinal transplant recipients. | recurrent bloodstream infections are a common indication for intestinal transplantation (itx). often, clinical symptoms may be absent in the setting of bacteremia, especially in patients with chronic liver disease. this study investigated the incidence and impact of positive blood cultures (bcx) obtained from central venous catheters used for total parenteral nutrition (tpn) in asymptomatic patients immediately prior to cadaveric itx. of 13 consecutive patients transplanted between november 2003 ... | 2006 | 16908244 |
| roles of cellular activation and sulfated glycans in haemophilus somnus adherence to bovine brain microvascular endothelial cells. | haemophilus somnus can cause a devastating fibrinopurulent meningitis with thrombotic vasculitis and encephalitis in cattle. the mechanisms used by h. somnus to migrate from the bloodstream into the central nervous system (cns) are unknown. in this study, we demonstrate that h. somnus adheres to, but does not invade, bovine brain endothelial cells (bbec) in vitro. the number of adherent h. somnus was significantly increased by prior activation of the bbec with tumor necrosis factor alpha (tnf-al ... | 2006 | 16926425 |
| surveillance bronchoscopy in children during the first year after lung transplantation: is it worth it? | since january 2002, routine surveillance bronchoscopy with bronchoalveolar lavage (bal) and transbronchial biopsy has been performed in all paediatric recipients of lung and heart-lung transplants at the great ormond street hospital for children, london, uk, using a newly revised treatment protocol. | 2006 | 16928706 |
| surveillance bronchoscopy in children during the first year after lung transplantation: is it worth it? | since january 2002, routine surveillance bronchoscopy with bronchoalveolar lavage (bal) and transbronchial biopsy has been performed in all paediatric recipients of lung and heart-lung transplants at the great ormond street hospital for children, london, uk, using a newly revised treatment protocol. | 2006 | 16928706 |
| contamination of multiple-dose vials in a veterinary hospital. | bacterial contamination of multiple-dose saline bottles and medication vials in a veterinary teaching hospital was evaluated. bacterial contamination was identified in 16/88 (18%) containers, with no difference in contamination between the large animal clinic, small animal clinic, and ruminant ambulatory clinic. contamination of multiple-dose containers containing substances for injection was common, and potential pathogens were present in many situations. while the clinical significance is not ... | 2006 | 16933556 |
| in vivo reversion to the wild-type beta-lactam resistance phenotype mediated by a plasmid carrying ampr and qnra1 in enterobacter cloacae. | resistance to beta-lactams and quinolones in two isogenic enterobacter cloacae isolates was studied. one was susceptible to cefoxitin and amoxicillin-clavulanate. the other one showed its natural beta-lactam resistance pattern. both isolates had a nonfunctional ampr regulator. however, within the second one, the presence of a plasmid carrying ampr and qnra1 allowed reversion to the wild-type beta-lactam resistance phenotype and decreased susceptibility to fluoroquinolones. | 2006 | 16940123 |
| identification of rnase 8 as a novel human antimicrobial protein. | the antimicrobial activity of the human rnase a superfamily member rnase 8 was evaluated. recombinant rnase 8 exhibited broad-spectrum microbicidal activity against potential pathogenic microorganisms (including multidrug-resistant strains) at micro- to nanomolar concentrations. thus, rnase 8 was identified as a novel antimicrobial protein and may contribute to host defense. | 2006 | 16940129 |
| a case with psoas abscess caused by citrobacter freundii. | 2006 | 16941269 | |
| functional characterization of is1999, an is4 family element involved in mobilization and expression of beta-lactam resistance genes. | is1999 and a point mutant derivative, is1999.2, have been described inserted upstream of emerging antibiotic resistance genes bla(veb-1) and bla(oxa-48). 5' rapid amplification of cdna ends experiments revealed that expression of these beta-lactamase genes was driven by the outward-directed promoter, p(out), located in the is1999 elements. these findings led us to study is1999-mediated gene mobilization. thus, the transposition properties of is1999 and of is1999-based composite transposons, made ... | 2006 | 16952941 |
| evaluation of the new vitek 2 extended-spectrum beta-lactamase (esbl) test for rapid detection of esbl production in enterobacteriaceae isolates. | extended-spectrum beta-lactamases (esbls) are a large, rapidly evolving group of enzymes that confer resistance to oxyimino cephalosporins and monobactams and are inhibited by clavulanate. rapid reliable detection of esbl production is a prerequisite for successful infection management and for monitoring resistance trends and implementation of intervention strategies. we evaluated the performance of the new vitek 2 esbl test system (biomérieux, inc, hazelwood, mo.) in the identification of esbl- ... | 2006 | 16954257 |
| comparison of api 20e and inva pcr for identification of salmonella enterica isolates from swine production units. | api 20e and inva pcr were evaluated for the identification of salmonella enterica isolates from swine farms. api 20e had the highest agreement with other tests at the 99.9% likelihood level. both tests had 100% sensitivity and 96% specificity compared to 16s rrna sequencing. compared to serotyping, both tests had 96% sensitivity; specificity was 86% for api 20e and 79% for inva pcr. | 2006 | 16954281 |
| effectiveness of cephalosporins in the sputum of patients with nosocomial bronchopneumonia. | nosocomial bronchopneumonia is a frequent complication in patients with chronic intratracheal intubation. despite targeted antibiotic treatment, production of abundant bronchial secretion containing pathogen bacteria often tends to be chronic, and so mortality drastically increases. this problem led to an investigation of the penetration of five cephalosporin antibiotics into the sputum. serum and sputum were collected from 24 chronically intubated patients having purulent nosocomial bronchopneu ... | 2006 | 16954290 |
| comparison of salmonella enterica serovar typhimurium lt2 and non-lt2 salmonella genomic sequences, and genotyping of salmonellae by using pcr. | genes of salmonella enterica serovar typhimurium lt2 expected to be specifically present in salmonella were selected using the basic local alignment search tool (blast) program. the 152 selected genes were compared with 11 genomic sequences of salmonella serovars, including salmonella enterica subsp. i and iiib and salmonella bongori (v), and were clustered into 17 groups by their comparison patterns. a total of 38 primer pairs were constructed to represent each of the 17 groups, and pcr was per ... | 2006 | 16957240 |
| detection of enterobacter sakazakii in dried infant milk formula by cationic-magnetic-bead capture. | enterobacter sakazakii has been associated with life-threatening infections in premature low-birth-weight infants. contaminated infant milk formula (imf) has been implicated in cases of e. sakazakii meningitis. quick and sensitive methods to detect low-level contamination sporadically present in imf preparations would positively contribute towards risk reduction across the infant formula food chain. here we report on the development of a simple method, combining charged separation and growth on ... | 2006 | 16957259 |
| epigenetic gene regulation in the bacterial world. | like many eukaryotes, bacteria make widespread use of postreplicative dna methylation for the epigenetic control of dna-protein interactions. unlike eukaryotes, however, bacteria use dna adenine methylation (rather than dna cytosine methylation) as an epigenetic signal. dna adenine methylation plays roles in the virulence of diverse pathogens of humans and livestock animals, including pathogenic escherichia coli, salmonella, vibrio, yersinia, haemophilus, and brucella. in alphaproteobacteria, me ... | 2006 | 16959970 |
| occurrence of qnra-positive clinical isolates in french teaching hospitals during 2002-2005. | bacteria harbouring the novel qnra plasmid-mediated mechanism of quinolone resistance have been described in different countries, but the frequency of their occurrence has not been investigated. in total, 1,468 clinical isolates of enterobacteriaceae with quinolone resistance or extended-spectrum beta-lactamase (esbl) phenotypes were collected from eight teaching hospitals in france during 2002-2005 and screened for qnra. overall, 28 isolates (22 enterobacter cloacae, three klebsiella pneumoniae ... | 2006 | 16961639 |
| invasive enterobacter sakazakii disease in infants. | enterobacter sakazakii kills 40%-80% of infected infants and has been associated with powdered formula. we analyzed 46 cases of invasive infant e. sakazakii infection to define risk factors and guide prevention and treatment. twelve infants had bacteremia, 33 had meningitis, and 1 had a urinary tract infection. compared with infants with isolated bacteremia, infants with meningitis had greater birthweight (2,454 g vs. 850 g, p = 0.002) and gestational age (37 weeks vs. 27.8 weeks, p = 0.02), and ... | 2006 | 16965695 |
| intracellular accumulation of linezolid in escherichia coli, citrobacter freundii and enterobacter aerogenes: role of enhanced efflux pump activity and inactivation. | the oxazolidinone class of antibiotics such as linezolid have a narrow spectrum of activity that targets gram-positive bacteria. we hypothesized that the poor activity of linezolid in gram-negative bacteria is in part caused by relatively low intracellular concentration due to efflux. | 2007 | 16971414 |
| first detection of the ambler class c 1 ampc beta-lactamase in citrobacter freundii by a new, simple double-disk synergy test. | we report on the first detection of an ampc-type ambler class c 1 (acc-1) beta-lactamase in citrobacter freundi isolated from a patient also harboring acc-1-producing escherichia coli and klebsiella pneumoniae. we propose a simple cefoxitin-based double-disk synergy test (ddst) for the specific detection of acc-1 in members of the family enterobacteriaceae, including natural ampc producers, in association with a cloxacillin-based ddst as a first-line ampc-type beta-lactamase screening test. | 2006 | 16971653 |
| prevalence and genetic analysis of plasmid-mediated quinolone resistance determinants qnra and qnrs in enterobacteriaceae isolates from a french university hospital. | the spread of plasmid-mediated quinolone resistance determinants qnra and qnrs was evaluated in a collection of 186 extended-spectrum beta-lactamase (esbl)-positive enterobacterial isolates from 2002 to 2005 and 185 nalidixic acid-resistant strains isolated during the first 6 months of 2005 at the bicêtre hospital, france. out of these 186 esbl-positive isolates, 2.2 and 1.6% carried a qnra1 and a qnrs1 determinant, respectively. the esbls associated with qnra1 were veb-1, shv-12, and ctx-m-1, w ... | 2006 | 16982792 |
| genetic environment of acquired bla(acc-1) beta-lactamase gene in enterobacteriaceae isolates. | we studied the genetic organization of bla(acc-1) in 14 isolates of enterobacteriaceae from france, tunisia, and germany. in a common ancestor, isecp1 was likely involved in the mobilization of this gene from the hafnia alvei chromosome to a plasmid. other genetic events involving insertion sequences (particularly is26), transposons (particularly tn1696), or suli-type integrons have occurred, leading to complex genetic environments. | 2006 | 16982793 |
| two-center collaborative evaluation of performance of the bd phoenix automated microbiology system for identification and antimicrobial susceptibility testing of gram-negative bacteria. | the performance of the bd phoenix automated microbiology system (bd diagnostic systems, sparks, md) was assessed for identification (id) and antimicrobial susceptibility testing (ast) of the majority of clinically encountered bacterial isolates in a european collaborative two-center trial. a total of 494 bacterial isolates including various species of the enterobacteriaceae and 110 nonfermentative gram-negative bacteria were investigated: of these, 385 were single patient isolates, and 109 were ... | 2006 | 17005752 |
| mercuric reductase gene transfer from soil to rumen bacteria. | conjugal transfer between soil bacterial population and microorganisms isolated from the rumen of herbivores from mercury-polluted area was investigated. the transfer of mera encoding mercury-resistance plasmids from soil bacteria enterobacter cloacae and enterococcus durans into two ruminal isolates citrobacter freundii and bacillus subtilis was observed. approximately the same frequency of mobilization in mating experiments was observed for both gram-negative (approximately 2.5 x 10(-8), trans ... | 2006 | 17007435 |
| expression, purification and crystallization of l-methionine gamma-lyase 2 from entamoeba histolytica. | l-methionine gamma-lyase (mgl) is considered to be an attractive target for rational drug development because the enzyme is absent in mammalian hosts. to enable structure-based design of drugs targeting mgl, one of the two mgl isoenzymes (ehmgl2) was crystallized in the orthorhombic space group p2(1)2(1)2(1), with unit-cell parameters a = 88.89, b = 102.68, c = 169.87 a. the crystal diffracted to a resolution of 2.0 a. the presence of a tetramer in the asymmetric unit (4 x 43.1 kda) gives a matt ... | 2006 | 17012806 |
| emergence of serine carbapenemases (kpc and sme) among clinical strains of enterobacteriaceae isolated in the united states medical centers: report from the mystic program (1999-2005). | among 8885 enterobacteriaceae tested in the 1999 to 2005 period as part of the usa meropenem yearly susceptibility test information collection (mystic) program, 51 strains with increased imipenem and meropenem mic values (> or =2 microg/ml) were detected. bla(kpc) was identified from 28 klebsiella pneumoniae from 3 medical centers in the new york city area (8 ribotypes), 2 klebsiella oxytoca from arkansas (same ribotype), 7 citrobacter freundii (6 from new york [5 ribotypes] and 1 from delaware) ... | 2006 | 17020798 |
| enumeration of bacterial cell numbers and detection of significant bacteriuria by use of a new flow cytometry-based device. | a new, automated flow cytometry-based urine bacterium analyzer (uba) was developed. we assessed the uba for linearity of measurement, reproducibility of results, carryover rate, and correlation of measured results with those determined by urine culture. we also evaluated its ability to screen urine samples for significant bacteriuria. the uba showed excellent linearity and a minor carryover rate. results from the uba were highly reproducible, and in between-run precision assays, the coefficients ... | 2006 | 17021088 |
| evaluation of the 10th external quality assessment scheme results in clinical microbiology laboratories in tehran and districts. | we evaluated the performance of microbiology laboratories in the 10th run of the external quality assessment scheme (eqas) in tehran and districts. each laboratory was sent 2 species of bacteria for identification. of the 487 laboratories that participated, 437 returned their findings. while 77.0% and 69.9% correctly identified staphylococcus saprophyticus and citrobacter freundii respectively, only 29.8% correctly identified acinetobacter baumanii, 25.3% identified enterococcus faecalis and 35. ... | 2006 | 17037699 |
| carbon : nitrogen : phosphorus ratios influence biofilm formation by enterobacter cloacae and citrobacter freundii. | to test the effects of c : n : p ratio modification of a well-known nutrient medium formulation, the endo formulation on biofilm formation by enterobacter cloacae ecl and citrobacter freundii cf1 in both single-species and binary species biofilms. | 2006 | 17040234 |
| bacterial identification, clinical significance, and antimicrobial susceptibilities of acinetobacter ursingii and acinetobacter schindleri, two frequently misidentified opportunistic pathogens. | the species belonging to the acinetobacter genus are currently reported as opportunistic pathogens in hospitalized patients with underlying predispositions. however, except for the acinetobacter calcoaceticus-acinetobacter baumannii complex, the identification of other species is frequently unreliable, especially for acinetobacter ursingii and acinetobacter schindleri, newly described in 2001. thus, the clinical significance, phenotypic features, and antimicrobial susceptibilities of these two m ... | 2006 | 17050816 |
| misidentification of a mucoid strain of salmonella enterica serotype choleraesuis as hafnia alvei by the vitek gni+ card system. | nontyphoidal salmonellae are among the most common causes of bacterial gastroenteritis worldwide. they are also notable causes of extraintestinal infections, including bacteremia and vascular infections. salmonella enterica serotype choleraesuis is typically associated with invasive infections. we report a patient who had an infected intra-abdominal aortic aneurysm due to an unusually mucoid strain of salmonella enterica serotype choleraesuis. the isolate was erroneously identified as hafnia alv ... | 2006 | 17050821 |
| [development of beta-lactamase resistance in enterobacteria]. | enterobacteria produce elementary chromosomal enzymes, beta-lactamases of class a: tem and shv (escherichia coli, klebsiella pneumoniae). these can give rise to plasmid-coded broad-spectrum beta-lactamases (esbl) discovered in 1980 (e. coli, k. pneumoniae, enterobacter cloacae). the first cefotaximase (ctx-m, men-1) was reported in europe in 1990. this enzyme is far more active against cefotaxime than against ceftazidime and aztreonam. chromosomal hyperpoduction of k1 beta-lactamase differs from ... | 2006 | 17051471 |
| high prevalence of ceftazidime-resistant klebsiella pneumoniae and increase of imipenem-resistant pseudomonas aeruginosa and acinetobacter spp. in korea: a konsar program in 2004. | a nationwide antimicrobial resistance surveillance has been conducted since 1997 in korea. in this study, susceptibility test data generated in 2004 by konsar group hospitals were analyzed and compared to those at a commercial laboratory. in hospitals, the rank orders of organisms in 2004 were identical to those in 2003. the most prevalent species was staphylococcus aureus (20.2%) in hospitals, but escherichia coli (29.7%) in the commercial laboratory. the proportions of enterococcus faecium to ... | 2006 | 17066507 |
| sensitive quantitative detection of commensal bacteria by rrna-targeted reverse transcription-pcr. | a sensitive rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) method was developed for exact and sensitive enumeration of subdominant bacterial populations. using group- or species-specific primers for 16s or 23s rrna, analytical curves were constructed for escherichia coli, enterococcus faecalis, staphylococcus aureus, clostridium perfringens, and pseudomonas aeruginosa, and the threshold cycle value was found to be linear up to an rna amount of 10(-3) cell per rt-pcr. the number o ... | 2007 | 17071791 |
| sensitive quantitative detection of commensal bacteria by rrna-targeted reverse transcription-pcr. | a sensitive rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) method was developed for exact and sensitive enumeration of subdominant bacterial populations. using group- or species-specific primers for 16s or 23s rrna, analytical curves were constructed for escherichia coli, enterococcus faecalis, staphylococcus aureus, clostridium perfringens, and pseudomonas aeruginosa, and the threshold cycle value was found to be linear up to an rna amount of 10(-3) cell per rt-pcr. the number o ... | 2007 | 17071791 |
| comparison of media for the isolation of enterobacter sakazakii. | enterobacter sakazakii is associated with neonatal infections and is occasionally present at low levels (<1 cfu/g) in powdered infant formula milk (ifm). it has been previously reported that some e. sakazakii strains do not grow in standard media for enterobacteriaceae and coliform bacteria; therefore, a reliable method is needed for recovery of the organism. three e. sakazakii enrichment broths-enterobacteriaceae enrichment broth (ee), e. sakazakii selective broth (essb), and modified lauryl su ... | 2007 | 17071794 |
| comparison of media for the isolation of enterobacter sakazakii. | enterobacter sakazakii is associated with neonatal infections and is occasionally present at low levels (<1 cfu/g) in powdered infant formula milk (ifm). it has been previously reported that some e. sakazakii strains do not grow in standard media for enterobacteriaceae and coliform bacteria; therefore, a reliable method is needed for recovery of the organism. three e. sakazakii enrichment broths-enterobacteriaceae enrichment broth (ee), e. sakazakii selective broth (essb), and modified lauryl su ... | 2007 | 17071794 |
| are laboratory-based antibiograms reliable to guide the selection of empirical antimicrobial treatment in patients with hospital-acquired infections? | antibiograms are often taken into account to define a rational selection of an empirical antimicrobial therapy for treating patients with hospital-acquired infections. in this study, we performed a paired comparison between the antibiogram constructed with laboratory-based data and that formed with data subjected to prior clinical validation. | 2007 | 17079239 |
| functional analysis of replication and stability regions of broad-host-range conjugative plasmid ctx-m3 from the incl/m incompatibility group. | plasmid ctx-m3 (89 kb) isolated from citrobacter freundii from a warsaw hospital is a mosaic plasmid with replication functions 100% identical with those of pmu407.1 of the incl/m group, conjugative operons with up to 60% homology to colib-p9 (inci) and stability functions originating either from nr1(r100) (incfii) or colib-p9 /r1/nr1 plasmids. we established the broad-host-range for pctx-m3 and defined its minireplicon in escherichia coli. we analyzed the role of stability cassettes and showed ... | 2007 | 17087993 |
| controlled clinical comparison of versatrek and bact/alert blood culture systems. | to assess the relative yields in automated microbial detection systems of bacteria and yeasts isolated from the blood of adult patients with suspected sepsis, we compared the new versatrek system (vti) (trek diagnostic systems, cleveland, oh) to the bact/alert 3d system (3d) (biomérieux, inc., durham, nc). identical protocols were followed for the two systems. paired aerobic (redox 1) and anaerobic (redox 2) vti media were compared with standard aerobic (sa) and anaerobic (sn) 3d media; each of ... | 2006 | 17122016 |
| controlled clinical comparison of versatrek and bact/alert blood culture systems. | to assess the relative yields in automated microbial detection systems of bacteria and yeasts isolated from the blood of adult patients with suspected sepsis, we compared the new versatrek system (vti) (trek diagnostic systems, cleveland, oh) to the bact/alert 3d system (3d) (biomérieux, inc., durham, nc). identical protocols were followed for the two systems. paired aerobic (redox 1) and anaerobic (redox 2) vti media were compared with standard aerobic (sa) and anaerobic (sn) 3d media; each of ... | 2006 | 17122016 |
| causes of community-acquired bacteremia and patterns of antimicrobial resistance in vientiane, laos. | there is no published information on the causes of bacteremia in the lao pdr (laos). between 2000 and 2004, 4512 blood culture pairs were taken from patients admitted to mahosot hospital, vientiane, laos, with suspected community-acquired bacteremia; 483 (10.7%) cultures grew a clinically significant community-acquired organism, most commonly salmonella enterica serovar typhi (50.9%), staphylococcus aureus (19.0%), and escherichia coli (12.4%). s. aureus bacteremia was common among infants (69.2 ... | 2006 | 17124000 |
| occurrence and diversity of integrons and beta-lactamase genes among ampicillin-resistant isolates from estuarine waters. | the aim of the present study was to assess the occurrence and molecular diversity of beta-lactamase genes and integrons among gram-negative ampicillin-resistant bacteria from ria de aveiro. ampicillin-resistant isolates were selected and subjected to genotyping using rep-pcr. representatives from each rep pattern were affiliated with the following taxa by sequencing the 16s rrna gene: aeromonas caviae, a. hydrophila, a. media, a. molluscorum, a. veronii, a. salmonicida, aeromonas sp., pseudomona ... | 2006 | 17125975 |
| molecular mechanism of the regulation of bacillus subtilis gltab expression by gltc. | in bacillus subilis, glutamate synthase, a major enzyme of nitrogen metabolism, is encoded by the gltab operon. significant expression of this operon requires the activity of gltc, a lysr-family protein, encoded by the divergently transcribed gene. we purified a soluble, active form of gltc and found that it requires alpha-ketoglutarate, a substrate of glutamate synthase, to fully activate glta transcription in vitro, and that its activity is inhibited by glutamate, the product of glutamate synt ... | 2007 | 17134717 |
| molecular mechanism of the regulation of bacillus subtilis gltab expression by gltc. | in bacillus subilis, glutamate synthase, a major enzyme of nitrogen metabolism, is encoded by the gltab operon. significant expression of this operon requires the activity of gltc, a lysr-family protein, encoded by the divergently transcribed gene. we purified a soluble, active form of gltc and found that it requires alpha-ketoglutarate, a substrate of glutamate synthase, to fully activate glta transcription in vitro, and that its activity is inhibited by glutamate, the product of glutamate synt ... | 2007 | 17134717 |
| transient neutrophilic thrombophagocytosis associated with citrobacter freundii septicemia. | 2006 | 17149945 | |
| messing with bacterial quorum sensing. | quorum sensing is widely recognized as an efficient mechanism to regulate expression of specific genes responsible for communal behavior in bacteria. several bacterial phenotypes essential for the successful establishment of symbiotic, pathogenic, or commensal relationships with eukaryotic hosts, including motility, exopolysaccharide production, biofilm formation, and toxin production, are often regulated by quorum sensing. interestingly, eukaryotes produce quorum-sensing-interfering (qsi) compo ... | 2006 | 17158701 |
| how phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria. | the phosphoenolpyruvate(pep):carbohydrate phosphotransferase system (pts) is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, amino sugars, polyols, and other sugar derivatives. to carry out its catalytic function in sugar transport and phosphorylation, the pts uses pep as an energy source and phosphoryl donor. the phosphoryl group of pep is usually transferred via four distinct proteins (domains) to the transported sugar bo ... | 2006 | 17158705 |
| a robotic dna purification protocol and real-time pcr for the detection of enterobacter sakazakii in powdered infant formulae. | enterobacter sakazakii is the causative agent of rare but severe food-borne infections associated with meningitis, necrotizing enterocolitis and sepsis in infants. rehydrated powdered infant formulae have been implicated as the source of infection in several outbreaks and sporadic cases. in this work, a real time fluorescence resonance energy transfer pcr assay incorporating an internal amplification control (iac) was developed for the specific detection of e. sakazakii in foods. performance of ... | 2006 | 17166252 |
| evaluation of a new selective chromogenic agar medium for detection of extended-spectrum beta-lactamase-producing enterobacteriaceae. | a novel chromogenic agar medium (esbl-bx; biomérieux, marcy l'etoile, france) was compared to macconkey agar supplemented with 2 mg ceftazidime/liter (mckc) for the selective isolation and presumptive identification of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae directly from clinical samples. of a total of 644 clinical specimens (including 551 fecal samples), 496 yielded no growth and 148 yielded growth on one or both media. overall, 44 esbl-producing enterobacteriaceae ... | 2007 | 17182756 |
| biodiversity of bacterial ecosystems in traditional egyptian domiati cheese. | bacterial biodiversity occurring in traditional egyptian soft domiati cheese was studied by pcr-temporal temperature gel electrophoresis (ttge) and pcr-denaturing gradient gel electrophoresis (dgge). bands were identified using a reference species database (j.-c. ogier et al., appl. environ. microbiol. 70:5628-5643, 2004); de novo bands having nonidentified migration patterns were identified by dna sequencing. results reveal a novel bacterial profile and extensive bacterial biodiversity in domia ... | 2007 | 17189434 |
| biodiversity of bacterial ecosystems in traditional egyptian domiati cheese. | bacterial biodiversity occurring in traditional egyptian soft domiati cheese was studied by pcr-temporal temperature gel electrophoresis (ttge) and pcr-denaturing gradient gel electrophoresis (dgge). bands were identified using a reference species database (j.-c. ogier et al., appl. environ. microbiol. 70:5628-5643, 2004); de novo bands having nonidentified migration patterns were identified by dna sequencing. results reveal a novel bacterial profile and extensive bacterial biodiversity in domia ... | 2007 | 17189434 |
| growth of salmonella enterica in model mixed cultures during a two-step enrichment. | growth of salmonella enterica was studied in model mixed cultures with citrobacter freundii or escherichia coli in buffered peptone water (bpw) and in rappaport-vassiliadis medium with soya (rvs) with modified concentrations of mgcl2 and malachite green, and at modified incubation temperatures. selected s. enterica strains were inoculated in bpw (10(0) cfu/ml) together with selected strains of citrobacter freundii (up to 10(8) cfu/ml) or selected strains of escherichia coli (up to 10(8) cfu/ml), ... | 2006 | 17201092 |
| evaluation of the merlin micronaut system for rapid direct susceptibility testing of gram-positive cocci and gram-negative bacilli from positive blood cultures. | bloodstream infections are life-threatening conditions which require the timely initiation of appropriate antimicrobial therapy. we evaluated the automated merlin micronaut system for rapid direct microtiter broth antimicrobial susceptibility testing (ast) of gram-positive cocci and gram-negative bacilli from bactec 9240 bottles with positive blood cultures in comparison to the standard method for the merlin micronaut system. this prospective study was conducted under routine working conditions ... | 2007 | 17202283 |
| modes and modulations of antibiotic resistance gene expression. | since antibiotic resistance usually affords a gain of function, there is an associated biological cost resulting in a loss of fitness of the bacterial host. considering that antibiotic resistance is most often only transiently advantageous to bacteria, an efficient and elegant way for them to escape the lethal action of drugs is the alteration of resistance gene expression. it appears that expression of bacterial resistance to antibiotics is frequently regulated, which indicates that modulation ... | 2007 | 17223624 |
| spontaneous citrobacter freundii infection in an immunocompetent patient. | 2007 | 17224563 | |
| chromosome-encoded narrow-spectrum ambler class a beta-lactamase gil-1 from citrobacter gillenii. | a novel beta-lactamase gene was cloned from the whole-cell dna of an enterobacterial citrobacter gillenii reference strain that displayed a weak narrow-spectrum beta-lactam-resistant phenotype and was expressed in escherichia coli. it encoded a clavulanic acid-inhibited ambler class a beta-lactamase, gil-1, with a pi value of 7.5 and a molecular mass of ca. 29 kda. gil-1 had the highest percent amino acid sequence identity with tem-1 and shv-1, 77%, and 67%, respectively, and only 46%, 31%, and ... | 2007 | 17242148 |
| multicenter beta trial of the genexpert enterovirus assay. | the genexpert dx system (cepheid, sunnyvale, ca) is a fully integrated and automated nucleic acid sample preparation, amplification, and real-time detection system. it consists of an instrument, a personal computer, and disposable fluidic cartridges. the analytical sensitivity and specificity of the genexpert enterovirus assay (gxea) were determined with a panel of 63 different enterovirus serotypes and 24 other microorganisms, respectively. the potential for blood, hemoglobin, white blood cells ... | 2007 | 17251395 |
| predictors of in-hospital mortality for bloodstream infections caused by enterobacter species or citrobacter freundii. | to identify predictors of in-hospital mortality among patients with bacteremia caused by enterobacter cloacae, enterobacter aerogenes, or citrobacter freundii. | 2007 | 17253909 |
| monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16s rrna gene-based analyses. | the diversity and dynamics of bacterial populations in saint-nectaire, a raw-milk, semihard cheese, were investigated using a dual culture-dependent and direct molecular approach combining single-strand conformation polymorphism (sscp) fingerprinting and sequencing of 16s rrna genes. the dominant clones, among 125 16s rrna genes isolated from milk, belonged to members of the firmicutes (58% of the total clones) affiliated mainly with the orders clostridiales and the lactobacillales, followed by ... | 2007 | 17259356 |
| erythromycin resistance-conferring plasmid prsb105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing tn402-like element, and a large region of unknown function. | the erythromycin resistance plasmid prsb105 was previously isolated from an activated sludge bacterial community of a municipal wastewater treatment plant. compilation of the complete prsb105 nucleotide sequence revealed that the plasmid is 57,137 bp in size and has a mean g+c content of 56.66 mol%. the prsb105 backbone is composed of two different replication and/or partitioning modules and a functional mobilization region encoding the mobilization genes mobcde and mobba. the first replicon (re ... | 2007 | 17261525 |
| first identification of pseudomonas aeruginosa isolates producing a kpc-type carbapenem-hydrolyzing beta-lactamase. | in medellin, colombia, three pseudomonas aeruginosa isolates with high-level carbapenem resistance (mic>or=256 microg/ml) and an isolate of citrobacter freundii with reduced susceptibility to imipenem produced the plasmid-mediated class a carbapenemase kpc-2. this is the first report of a kpc-type beta-lactamase identified outside of the family enterobacteriaceae. | 2007 | 17261621 |
| in vitro and in vivo activities of pd 0305970 and pd 0326448, new bacterial gyrase/topoisomerase inhibitors with potent antibacterial activities versus multidrug-resistant gram-positive and fastidious organism groups. | pd 0305970 and pd 0326448 are new bacterial gyrase and topoisomerase inhibitors (quinazoline-2,4-diones) that possess outstanding in vitro and in vivo activities against a wide spectrum of bacterial species including quinolone- and multidrug-resistant gram-positive and fastidious organism groups. the respective mics (microg/ml) for pd 0305970 capable of inhibiting>or=90% of bacterial strains tested ranged from 0.125 to 0.5 versus staphylococci, 0.03 to 0.06 versus streptococci, 0.25 to 2 versus ... | 2007 | 17261623 |
| surveillance of bacteriological quality of natural water resources in rural areas around kasauli town, distt. solan, himachal pradesh. | a study was conducted to find out the bacteriological quality of water during the monsoon season in local areas around kasauli town, and to evaluate the management of water resources in rural areas. the water samples were collected from bowries and hand pumps. as there is no municipal water supply in majority of these areas, the local residents depends mainly on these natural resources without any treatment of water. all the 14 surface water samples collected from bowries were found bacteriologi ... | 2005 | 17278659 |
| detection of extended-spectrum beta-lactamases among enterobacteriaceae by use of semiautomated microbiology systems and manual detection procedures. | three commercially available microbiology identification and susceptibility testing systems were compared with regard to their ability to detect extended-spectrum beta-lactamase (esbl) production in enterobacteriaceae, i.e., the phoenix automated microbiology system (bd diagnostic systems, sparks, md), the vitek 2 system (biomérieux, marcy l'etoile, france), and the microscan walkaway-96 system (dade behring, inc., west sacramento, ca), using routine testing panels. one hundred fifty putative es ... | 2007 | 17287329 |
| influence of amino acids on low-density escherichia coli responses to nutrient downshifts. | a vast bibliography on nutrient effects on high-density cultures exists, while it has been overlooked that low densities of starving cells are often the rule in natural environments. by means of a novel sensitive beta-galactosidase assay, we examined escherichia coli transitions to minimal media when the cell concentration was 100 to 10,000 cells per ml. as in high-density cultures, the enzyme activity depended on amino acid availability and was subject to catabolite repression and stringent con ... | 2007 | 17293414 |
| [antagonistic effect of bacteriocinogenic lactobacillus acidophilus on klebsiella pneumoniae, citrobacter freundii and proteus mirabilis cells]. | data of the ultrastructural cellular changes of conditionally pathogenic enterobacteria, including k. pneumoniae, c. freundii and p. mirabilis cells impacts to bacteriocin-producing l. acidophilus are presented. enterobacteria in response to the bacteriocinogenic effect of lactobacilli are manifestated by expressive destructions of sensitive to pore formation bacteriocin cells. various morphological types of enterobacteria cells with increase of involution, lysing and resting forms are revealed. ... | 2006 | 17297872 |
| evaluation of phoenix automated microbiology system for detecting extended-spectrum beta-lactamases among chromosomal ampc-producing enterobacter cloacae, enterobacter aerogenes, citrobacter freundii, and serratia marcescens. | we evaluated the bd phoenix extended-spectrum beta-lactamase (esbl) detection test among chromosomal ampc-producing enterobacter cloacae, enterobacter aerogenes, citrobacter freundii, and serratia marcescens. the study was conducted on 72 non-repetitive esbl producers (33 e. cloacae, 13 e. aerogenes, 14 c. freundii, and 12 s. marcescens) and 77 esbl non-producers (33 e. cloacae, 9 e. aerogenes, 6 c. freundii, and 29 s. marcescens). the organisms were selected as suspected esbl-producers based on ... | 2007 | 17311873 |
| resistance to gram-negative organisms due to high-level expression of plasmid-encoded ampc beta-lactamase blacmy-4 promoted by insertion sequence isecp1. | a klebsiella pneumoniae strain, ku6500, which showed resistance to extended-spectrum beta-lactams and produced the plasmid-encoded ampc beta-lactamase cmy-4, was identified from clinical isolates in japan. the aim of this study was to identify the mechanism of the high-level expression of blacmy-4. sequence analysis indicated that the promoter element of citrobacter freundii was conserved, but the insertion sequence isecp1 coding with the putative promoter element, was inserted into the ampr bin ... | 2007 | 17334724 |
| influence of vitamin b12 and cocultures on the growth of dehalococcoides isolates in defined medium. | bacteria belonging to the genus dehalococcoides play a key role in the complete detoxification of chloroethenes as these organisms are the only microbes known to be capable of dechlorination beyond dichloroethenes to vinyl chloride (vc) and ethene. however, dehalococcoides strains usually grow slowly with a doubling time of 1 to 2 days and have complex nutritional requirements. here we describe the growth of dehalococcoides ethenogenes 195 in a defined mineral salts medium, improved growth of st ... | 2007 | 17337553 |
| development of a simple latex agglutination assay for detection of shiga toxin-producing escherichia coli (stec) by using polyclonal antibody against stec. | rabbit antiserum raised against the whole-cell antigen of shiga toxin-producing escherichia coli (stec) strain vt3 (stx1+ stx2+ eae+) was repeatedly adsorbed with heat-killed cells of different non-stec strains and other enteric bacteria. thus, the antiserum obtained was designated vt3 antiserum. vt3 antiserum reacted with intimin type gamma. we assessed the reactivity of vt3 antiserum to whole-cell lysates of 87 strains of e. coli and other enteric bacteria by immunoblotting. the antiserum reco ... | 2007 | 17344348 |
| colicin biology. | colicins are proteins produced by and toxic for some strains of escherichia coli. they are produced by strains of e. coli carrying a colicinogenic plasmid that bears the genetic determinants for colicin synthesis, immunity, and release. insights gained into each fundamental aspect of their biology are presented: their synthesis, which is under sos regulation; their release into the extracellular medium, which involves the colicin lysis protein; and their uptake mechanisms and modes of action. co ... | 2007 | 17347522 |
| reevaluation of clinical and laboratory standards institute disk diffusion breakpoints for tetracyclines for testing enterobacteriaceae. | we reevaluated enterobacteriaceae disk diffusion breakpoints for the tetracyclines published in the clinical and laboratory standards institute (clsi) document m100-s16, which were (susceptible/resistant) >or=19 mm/<or=14 mm for tetracycline, >or=16 mm/<or=12 mm for doxycycline, and >or=19 mm/<or=14 mm for minocycline. a collection of 504 recent clinical isolates of enterobacteriaceae were tested against these tetracycline compounds by disk diffusion and broth microdilution methods according to ... | 2007 | 17360844 |
| first detection of a vim-1 metallo-beta-lactamase in a carbapenem-resistant citrobacter freundii clinical isolate in an acute hospital in germany. | we report the first detection of a carbapenem-resistant citrobacter freundii clinical strain in germany. it was isolated from an abscess of a patient with acute necrotic pancreatitis in an acute hospital. pcr and sequencing experiments revealed that the carbapenem resistance was mediated by a vim-1 metallo-beta-lactamase, located on a plasmid encoded class 1 integron. carbapenem resistance in enterobacteriaceae is so far a rare event and 1 of the major therapeutic concerns in the future. | 2007 | 17366062 |
| dissemination and persistence of a plasmid-mediated tem-3-like beta-lactamase, tem-139, among enterobacteriaceae in bulgaria. | during a survey of extended-spectrum beta-lactamases (esbls) in bulgaria from 1996 to 2003, a tem-3-like esbl was detected in strains of klebsiella pneumoniae, escherichia coli, citrobacter freundii and klebsiella oxytoca from three centres in three different towns. the nucleotide sequence of the cloned gene was identical to that of tem-3, except for one substitution (c347a) causing an amino acid exchange at position 49 from leucine to methionine. this tem-3 variant with both a unique nucleotide ... | 2007 | 17382521 |
| development of an advanced electrochemical dna biosensor for bacterial pathogen detection. | electrochemical sensors have the capacity for rapid and accurate detection of a wide variety of target molecules in biological fluids. we have developed an electrochemical sensor assay involving hybridization of bacterial 16s rrna to fluorescein-modified detector probes and to biotin-modified capture probes anchored to the sensor surface. signal is generated by an oxidation-reduction current produced by the action of horseradish peroxidase conjugated to an anti-fluorescein monoclonal fab. a prev ... | 2007 | 17384207 |
| high-frequency phage-mediated gene transfer among escherichia coli cells, determined at the single-cell level. | recent whole-genome analysis suggests that lateral gene transfer by bacteriophages has contributed significantly to the genetic diversity of bacteria. to accurately determine the frequency of phage-mediated gene transfer, we employed cycling primed in situ amplification-fluorescent in situ hybridization (cprins-fish) and investigated the movement of the ampicillin resistance gene among escherichia coli cells mediated by phage at the single-cell level. phages p1 and t4 and the newly isolated e. c ... | 2007 | 17384307 |
| preservation of integron types among enterobacteriaceae producing extended-spectrum beta-lactamases in a spanish hospital over a 15-year period (1988 to 2003). | the variable presence of integrons among extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae species (0 to 66%) is described. association between bla(esbl) and integrons occurred when these are linked to specific esbl-type genes (in60 bearing iscr1 and bla(ctx-m-9)) or when esbl genes were superimposed onto selected plasmids carrying integrons. some integrons were identical to those found during decades worldwide, illustrating the preservation of the genetic elements carrying th ... | 2007 | 17404002 |
| microbiologic profile of flexible endoscope disinfection in two brazilian hospitals. | [corrected] endoscopes are routinely used in hospitals and clinics of the world and they can be potential sources of cross-infection when the decontamination process is unsuitable | 2006 | 17406750 |
| characteristics of beta-lactamases and their genes (blaa and blab) in yersinia intermedia and y. frederiksenii. | the presence of beta-lactamases in y. enterocolitica has been reported to vary with serovars, biovars and geographical origin of the isolates. an understanding of the beta-lactamases in other related species is important for an overall perception of antibiotic resistance in yersiniae. the objective of this work was to study the characteristics of beta-lactamases and their genes in strains of y. intermedia and y. frederiksenii, isolated from clinical and non-clinical sources in india. | 2007 | 17407578 |
| automatic mapping of viable microbial cells distributed in the surface layer of cotton fabrics. | viable microbial cells distributed in a 130 microim thick surface layer of cotton fabrics were stained with a fluorescent glucose, 2- [n- (7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino] -2-deoxy-d-glucose (2-nbdg), and automatically mapped with an ultra-deep focusing range microscope (udf) system. the software of the udf system was upgraded and the number of candida albicans cells could be counted at a higher precision than before. bacterial cells of pseudomonas fluorescens, serratia marcescens, and ... | 2007 | 17408006 |
| repetitive prostatic massage and drug therapy as an alternative to transurethral resection of the prostate. | we describe 5 men with urinary retention and indwelling urethral catheters who were treated with repetitive prostatic massage, antimicrobials, alpha blockers, and--in 2 cases--finasteride. we retrospectively reviewed the charts of all patients presenting to the genitourinary clinic with indwelling urinary catheters during a 1-year period. five men (mean age, 70 years; range, 64-76; sd 4.47) presented to the manila genitourinary clinic (cebu branch), cebu, philippines, wearing indwelling urinary ... | 2006 | 17415302 |
| in vitro activities of cg400549, a novel fabi inhibitor, against recently isolated clinical staphylococcal strains in korea. | the in vitro activities of cg400549, a novel fabi inhibitor, were compared to those of linezolid and commonly used antimicrobials against recent bacterial isolates. cg400549 had an mic(90) of 0.5 microg/ml for staphylococcus aureus strains and was more potent than either linezolid or vancomycin. | 2007 | 17420210 |