Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| structure of the catalytic domain of the tannerella forsythia matrix metallopeptidase karilysin in complex with a tetrapeptidic inhibitor. | karilysin is the only metallopeptidase identified as a virulence factor in the odontopathogen tannerella forsythia owing to its deleterious effect on the host immune response during bacterial infection. the very close structural and sequence-based similarity of its catalytic domain (kly18) to matrix metalloproteinases suggests that karilysin was acquired by horizontal gene transfer from an animal host. previous studies by phage display identified peptides with the consensus sequence xwfpxxxggg ( ... | 2013 | 23695557 |
| high-resolution transcriptome maps reveal strain-specific regulatory features of multiple campylobacter jejuni isolates. | campylobacter jejuni is currently the leading cause of bacterial gastroenteritis in humans. comparison of multiple campylobacter strains revealed a high genetic and phenotypic diversity. however, little is known about differences in transcriptome organization, gene expression, and small rna (srna) repertoires. here we present the first comparative primary transcriptome analysis based on the differential rna-seq (drna-seq) of four c. jejuni isolates. our approach includes a novel, generic method ... | 2013 | 23696746 |
| periodontitis in pregnant baboons: systemic inflammation and adaptive immune responses and pregnancy outcomes in a baboon model. | chronic periodontal infections have been suggested to contribute to the risk of adverse pregnancy outcomes. | 2013 | 23710643 |
| periodontitis in pregnant baboons: systemic inflammation and adaptive immune responses and pregnancy outcomes in a baboon model. | chronic periodontal infections have been suggested to contribute to the risk of adverse pregnancy outcomes. | 2013 | 23710643 |
| effect of periodontal therapy on the subgingival microbiota over a 2-year monitoring period. i. overall effect and kinetics of change. | to examine the 2-year post-therapy kinetics of change in the composition of subgingival biofilms. | 2013 | 23710672 |
| porphyromonas gingivalis, treponema denticola and toll-like receptor 2 are associated with hypertensive disorders in placental tissue: a case-control study. | to explore the associations between the presence of periodontal pathogens and the expression of toll-like receptors (tlr-2 and tlr-4) in the placental tissue of patients with hypertensive disorders compared to the placentas of healthy normotensive patients. | 2013 | 23711357 |
| gingipain-dependent degradation of mammalian target of rapamycin pathway proteins by the periodontal pathogen porphyromonas gingivalis during invasion. | porphyromonas gingivalis and tannerella forsythia are gram-negative pathogens strongly associated with periodontitis. their abilities to interact, invade and persist within host cells are considered crucial to their pathogenicity, but the mechanisms by which they subvert host defences are not well understood. in this study, we set out to investigate whether p. gingivalis and t. forsythia directly target key signalling molecules that may modulate the host cell phenotype to favour invasion and per ... | 2013 | 23714361 |
| as-yet-uncultivated oral bacteria: breadth and association with oral and extra-oral diseases. | it has been shown that 40-60% of the bacteria found in different healthy and diseased oral sites still remain to be grown in vitro, phenotypically characterized, and formally named as species. the possibility exists that these as-yet-uncultivated bacteria play important ecological roles in oral bacterial communities and may participate in the pathogenesis of several oral infectious diseases. there is also a potential for these as-yet-uncultivated oral bacteria to take part in extra-oral infectio ... | 2013 | 23717756 |
| serum antibody responses to periodontal microbiota in chronic and aggressive periodontitis: a postulate revisited. | the authors revisited the 1999 international workshop postulate of robust serum antibody responses to infecting agents in localized aggressive periodontitis (lagp) and weak responses in generalized aggressive periodontitis (gagp). antibody responses were further examined in localized and generalized chronic periodontitis (lcp and gcp). | 2014 | 23725029 |
| spanner: taxonomic assignment of sequences using pyramid matching of similarity profiles. | homology-based taxonomic assignment is impeded by differences between the unassigned read and reference database, forcing a rank-specific classification to the closest (and possibly incorrect) reference lineage. this assignment may be correct only to a general rank (e.g. order) and incorrect below that rank (e.g. family and genus). algorithms like lca avoid this by varying the predicted taxonomic rank based on matches to a set of taxonomic references. lca and related approaches can be conservati ... | 2013 | 23732273 |
| bacterial sex in dental plaque. | genes are transferred between bacteria in dental plaque by transduction, conjugation, and transformation. membrane vesicles can also provide a mechanism for horizontal gene transfer. dna transfer is considered bacterial sex, but the transfer is not parallel to processes that we associate with sex in higher organisms. several examples of bacterial gene transfer in the oral cavity are given in this review. how frequently this occurs in dental plaque is not clear, but evidence suggests that it affe ... | 2013 | 23741559 |
| plaque retention on elastomeric ligatures. an in vivo study. | fixed orthodontic appliances make it difficult to maintain the oral hygiene, resulting in plaque accumulation. retention of bacterial plaque, represents a risk for white spot lesions and development of periodontal disease. | 2012 | 23741603 |
| plaque retention on elastomeric ligatures. an in vivo study. | fixed orthodontic appliances make it difficult to maintain the oral hygiene, resulting in plaque accumulation. retention of bacterial plaque, represents a risk for white spot lesions and development of periodontal disease. | 2012 | 23741603 |
| isolation of treponema and tannerella spp. from equine odontoclastic tooth resorption and hypercementosis related periodontal disease. | red complex bacteria, i.e. porphyromonas gingivalis, treponema denticola and tannerella forsythia, are involved in the onset and progression of periodontal disease in man, yet seldom inhabit the oral cavity of healthy individuals. periodontal disease is also encountered in horses, with equine odontoclastic tooth resorption and hypercementosis (eotrh) constituting a particular form of disease. however, only little is known about the oral microbiome of healthy and periodontitis-affected equids. | 2014 | 23742079 |
| differential expression of mitogen activating protein kinases in periodontitis. | following toll-like receptor (tlr) engagement, lipopolysaccharide (lps) can stimulate the expression of pro-inflammatory cytokines thus activating the innate immune response. the production of inflammatory cytokines results, in part, from the activation of kinase-induced signalling cascades and transcriptional factors. of the four distinct classes of mitogen-activated protein kinases (mapk) described in mammals, p38, c-jun n-terminal activated kinases (jnk1-3) and extracellular activated kinases ... | 2013 | 23742695 |
| periodontopathogen profile of healthy and oral lichen planus patients with gingivitis or periodontitis. | oral lichen planus (olp) is a chronic inflammatory disease that is frequently detected in oral tissues. the aim of our study was to identify the prevalence of the detection of periodontopathogenic microorganisms (aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia and treponema denticola in olp patients and to compare with this prevalence of periodontopathogenic microorganisms in healthy non-olp patients. our study included 27 (18 chronic ... | 2013 | 23743616 |
| [analysis of antimicrobial effect of mtad solution in infected root canal system using pcr technique]. | clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. | 2013 | 23745336 |
| clinical evaluation of salivary periodontal pathogen levels by real-time polymerase chain reaction in patients before dental implant treatment. | periodontal pathogens in dental plaque are the main causative agents of periodontitis and peri-implantitis. detection of the presence of such periodontal pathogens early would serve as a useful tool in the diagnosis and treatment of this disease. therefore, the purpose of this study was to investigate whether the periodontal pathogen levels in saliva were correlated with the periodontal status of patients receiving implant treatment. | 2014 | 23745964 |
| clinical, microbiologic, and host response characteristics in patients with peri-implant mucositis. | to analyze the clinical, microbiologic, and host response characteristics (interleukins 1β and 6) in the peri-implant sulcus fluid (pisf) of patients with healthy dental implants and with peri-implant mucositis. | 2013 | 23748323 |
| periodontopathogens levels and clinical response to periodontal therapy in individuals with the interleukin-4 haplotype associated with susceptibility to chronic periodontitis. | periodontitis is an inflammatory disease that results from an interaction between dental biofilm agents and the host immune-inflammatory response. periodontopathogenic organisms, such as porphyromonas gingivalis, tannerella forsythia, and treponema denticola, as well as the host's susceptibility, represented by the host's genetic makeup, are the key factors that influence this complex disease. recently, we identified haplotypes in the il4 gene that were associated with chronic periodontitis (cp) ... | 2013 | 23748736 |
| [detection of periodontal pathogens from saliva of type 2 diabetic patients in urban area of beijing]. | to investigate the prevalence of periodontal pathogens from saliva of patients with type 2 diabetes mellitus (t2dm), and to characterize the association between the glucose status and periodontal pathogens in oral cavity. | 2013 | 23751528 |
| treponema denticola major outer sheath protein impairs the cellular phosphoinositide balance that regulates neutrophil chemotaxis. | the major outer sheath protein (msp) of treponema denticola inhibits neutrophil polarization and directed chemotaxis together with actin dynamics in vitro in response to the chemoattractant n-formyl-methionine-leucine-phenylanine (fmlp). msp disorients chemotaxis through inhibition of a rac1-dependent signaling pathway, but the upstream mechanisms are unknown. we challenged murine bone marrow neutrophils with enriched native msp to determine the role of phospholipid modifying enzymes in chemotax ... | 2013 | 23755300 |
| the connection between ruptured cerebral aneurysms and odontogenic bacteria. | patients with ruptured saccular intracranial aneurysms have excess long-term mortality due to cerebrovascular and cardiovascular diseases compared with general population. chronic inflammation is detected in ruptured intracranial aneurysms, abdominal aortic aneurysms and coronary artery plaques. bacterial infections have been suggested to have a role in the aetiology of atherosclerosis. bacteria have been detected both in abdominal and coronary arteries but their presence in intracranial aneurys ... | 2013 | 23761916 |
| the biological activity of propolis-containing toothpaste on oral health environment in patients who underwent implant-supported prosthodontic rehabilitation. | the soft and periodontal tissues surrounding dental implants are particularly susceptible to bacteria invasion and inflammatory reactions due to complex histological structures. this study was carried out to investigate the influence of a propolis-containing hygienic agent on selected oral health parameters, oral microflora, and the condition of periodontal health. sixteen subjects who underwent an oral rehabilitation with dental implants were selected and randomly assigned into two groups, whic ... | 2013 | 23762153 |
| oral microbiome of deep and shallow dental pockets in chronic periodontitis. | we examined the subgingival bacterial biodiversity in untreated chronic periodontitis patients by sequencing 16s rrna genes. the primary purpose of the study was to compare the oral microbiome in deep (diseased) and shallow (healthy) sites. a secondary purpose was to evaluate the influences of smoking, race and dental caries on this relationship. a total of 88 subjects from two clinics were recruited. paired subgingival plaque samples were taken from each subject, one from a probing site depth > ... | 2013 | 23762384 |
| predictive, preventive, personalised and participatory periodontology: 'the 5ps age' has already started. | an impressive progress in dentistry has been recorded in the last decades. in order to reconsider guidelines in dentistry, it is required to introduce new concepts of personalised patient treatments: the wave of predictive, preventive and personalised medicine is rapidly incoming in dentistry. worldwide dentists have to make a big cultural effort in changing the actual 'reactive' therapeutic point of view, belonging to the last century, into a futuristic 'predictive' one. the first cause of toot ... | 2013 | 23763842 |
| interaction of human dopa decarboxylase with l-dopa: spectroscopic and kinetic studies as a function of ph. | human dopa decarboxylase (hddc), a pyridoxal 5'-phosphate (plp) enzyme, displays maxima at 420 and 335 nm and emits fluorescence at 384 and 504 nm upon excitation at 335 nm and at 504 nm when excited at 420 nm. absorbance and fluorescence titrations of hddc-bound coenzyme identify a single pk(spec) of ~7.2. this pk(spec) could not represent the ionization of a functional group on the schiff base but that of an enzymic residue governing the equilibrium between the low- and the high-ph forms of th ... | 2013 | 23781496 |
| a consortium of aggregatibacter actinomycetemcomitans, streptococcus parasanguinis, and filifactor alocis is present in sites prior to bone loss in a longitudinal study of localized aggressive periodontitis. | aggregatibacter actinomycetemcomitans-induced localized aggressive periodontitis (lap) in african-american adolescents has been documented but is poorly understood. two thousand fifty-eight adolescents aged 11 to 17 years were screened for their periodontal status and the presence of a. actinomycetemcomitans in their oral cavity. seventy-one a. actinomycetemcomitans-negative and 63 a. actinomycetemcomitans-positive periodontally healthy subjects were enrolled, sampled, examined, and radiographed ... | 2013 | 23784124 |
| inactivation of cyclic di-gmp binding protein tde0214 affects the motility, biofilm formation, and virulence of treponema denticola. | as a ubiquitous second messenger, cyclic dimeric gmp (c-di-gmp) has been studied in numerous bacteria. the oral spirochete treponema denticola, a periodontal pathogen associated with human periodontitis, has a complex c-di-gmp signaling network. however, its function remains unexplored. in this report, a pilz-like c-di-gmp binding protein (tde0214) was studied to investigate the role of c-di-gmp in the spirochete. tde0214 harbors a pilz domain with two signature motifs: rxxxr and dxsxxg. biochem ... | 2013 | 23794624 |
| a high-throughput microfluidic dental plaque biofilm system to visualize and quantify the effect of antimicrobials. | few model systems are amenable to developing multi-species biofilms in parallel under environmentally germane conditions. this is a problem when evaluating the potential real-world effectiveness of antimicrobials in the laboratory. one such antimicrobial is cetylpyridinium chloride (cpc), which is used in numerous over-the-counter oral healthcare products. the aim of this work was to develop a high-throughput microfluidic system that is combined with a confocal laser scanning microscope (clsm) t ... | 2013 | 23800904 |
| ornithine cyclodeaminase-based proline production by corynebacterium glutamicum. | the soil bacterium corynebacterium glutamicum, best known for its glutamate producing ability, is suitable as a producer of a variety of bioproducts. glutamate is the precursor of the amino acid proline. proline biosynthesis typically involves three enzymes and a spontaneous cyclisation reaction. alternatively, proline can be synthesised from ornithine, an intermediate of arginine biosynthesis. the direct conversion of ornithine to proline is catalysed by ornithine cyclodeaminase. an ornithine o ... | 2013 | 23806148 |
| a surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochaete treponema denticola. | neuraminidases (sialidases) catalyse the removal of terminal sialic acid from glycoconjugates. bacterial pathogens often utilize neuraminidases to scavenge host sialic acid, which can be utilized either as a nutrient or as a decorating molecule to disguise themselves from host immune attacks. herein, a putative neuraminidase (tde0471) was identified in treponema denticola, an oral spirochaete associated with human periodontitis. tde0471 is a cell surface-exposed exo-neuraminidase that removes si ... | 2013 | 23808705 |
| microflora and periodontal disease. | periodontitis is a disease that affects and destroys the tissues that support teeth. tissue damage results from a prolonged inflammatory response to an ecological shift in the composition of subgingival biofilms. three bacterial species that constitute the red complex group, porphyromonas gingivalis, tannerella forsythia, and treponema denticola, are considered the main pathogens involved in periodontitis. | 2012 | 23814584 |
| eubacterial spovg homologs constitute a new family of site-specific dna-binding proteins. | a site-specific dna-binding protein was purified from borrelia burgdorferi cytoplasmic extracts, and determined to be a member of the highly conserved spovg family. this is the first time a function has been attributed to any of these ubiquitous bacterial proteins. further investigations into spovg orthologues indicated that the staphylococcus aureus protein also binds dna, but interacts preferentially with a distinct nucleic acid sequence. site-directed mutagenesis and domain swapping between t ... | 2013 | 23818957 |
| pathogen levels and clinical response to periodontal treatment in patients with interleukin 8 haplotypes. | the aim of this study was to investigate the effect of non-surgical treatment of periodontitis on the levels of periodontopathogens and clinical parameters in patients with different genetic backgrounds produced by polymorphisms in the interleukin ( il8) gene. thirty patients grouped according to il8 atc/ttc or agt/ttc haplotypes were submitted to non-surgical periodontal treatment. levels of porphyromonas gingivalis, tannerella forsythia, and treponema denticola were determined in 240 subgingiv ... | 2013 | 23821559 |
| quantification of key periodontal pathogens in insulin-dependent type 2 diabetic and non-diabetic patients with generalized chronic periodontitis. | periodontitis is a common problem in patients with diabetes mellitus (dm), however, differences in the putative periodontal pathogens in subjects with dm compared to non-dm subjects are still inconclusive. the red complex, which includes porphyromonas gingivalis, treponema denticola, and tannerella forsythia, encompasses the most important pathogens in adult periodontal disease. the aim of the present study was to compare cell numbers of p. gingivalis, t. denticola, t. forsythia and aggregatibac ... | 2013 | 23827459 |
| periodontal disease: a covert source of inflammation in chronic kidney disease patients. | the prevalence of atherosclerotic complications (myocardial infarction, stroke, and sudden death) is increased in end-stage renal disease (esrd) patients, especially in haemodialysis patients. increasing evidence suggests that both in general population and in dialysis patients, systemic inflammation plays a dominant role in the pathogenesis of atherosclerotic complications. in general population, also, evidence shows that moderate to severe periodontitis can contribute to inflammatory burden by ... | 2013 | 23840952 |
| role of bacterial infections in pancreatic cancer. | established risk factors for pancreatic cancer, including tobacco smoking, chronic pancreatitis, obesity and type 2 diabetes, collectively account for less than half of all pancreatic cancer cases. inflammation plays a key role in pancreatic carcinogenesis, but it is unclear what causes local inflammation, other than pancreatitis. epidemiological data suggest that helicobacter pylori may be a risk factor for pancreatic cancer, and more recently, data suggest that periodontal disease, and porphyr ... | 2013 | 23843038 |
| the unique hmuy gene sequence as a specific marker of porphyromonas gingivalis. | porphyromonas gingivalis, a major etiological agent of chronic periodontitis, acquires heme from host hemoproteins using the hmuy hemophore. the aim of this study was to develop a specific p. gingivalis marker based on a hmuy gene sequence. subgingival samples were collected from 66 patients with chronic periodontitis and 40 healthy subjects and the entire hmuy gene was analyzed in positive samples. phylogenetic analyses demonstrated that both the amino acid sequence of the hmuy protein and the ... | 2013 | 23844074 |
| monitoring the prevalence of viable and dead cariogenic bacteria in oral specimens and in vitro biofilms by qpcr combined with propidium monoazide. | streptococcus mutans and streptococcus sobrinus are associated with the development of dental caries in humans. however, previous diagnostic systems are unsuitable for monitoring viable cell numbers in oral specimens. assessing the relationship between the numbers of viable and dead bacterial cells and oral status is important for understanding oral infectious diseases. propidium monoazide (pma) has been reported to penetrate dead cells following membrane damage and to cross-link dna, thereby in ... | 2013 | 23848601 |
| comparative evaluation of locally delivered minocycline and metronidazole in the treatment of periodontitis. | the aim of this study was to evaluate and compare the efficacy of subgingivally delivered minocycline microspheres and 25% metronidazole gel when used as an adjunct to scaling and root planing (srp) in the treatment of chronic periodontitis. | 2013 | 23853452 |
| multilevel analysis of bacterial counts from chronic periodontitis after root planing/scaling, surgery, and systemic and local antibiotics: 2-year results. | to follow changes (over 2 years) in subgingival bacterial counts of five microbial complexes including health-related actinomyces spp. in deeper pockets (≥5 mm) after periodontal treatments. | 2013 | 23853701 |
| characterization of periodontal biofilm in down syndrome patients: a comparative study. | the aim of this study was to characterize the main periodontal bacterial species in down syndrome (ds) patients with and without periodontitis. | 2013 | 23855174 |
| anticardiolipin in porphyromonas gingivalis antisera causes fetal loss in mice. | β2-glycoprotein i (β2gpi)-dependent anticardiolipin autoantibodies (acl) are associated with thrombosis and fetal loss. some microbial pathogens can induce pathogenic antibodies cross-reactive with β2gpi. sera from a significant percentage of periodontitis patients contain acl, and some periodontal pathogens contain antigens with peptide sequences having homology to β2gpi. we hypothesized that antibodies raised against p. gingivalis (apg) contain pathogenic acl that induce fetal resorption. we i ... | 2013 | 23857644 |
| occurrence of red complex microorganisms and aggregatibacter actinomycetemcomitans in patients with diabetes. | the aim of the present study was to analyze the occurrence of porphyromonas gingivalis (p. gingivalis), tannerella forsythia (t. forsythia), treponema denticola (t. denticola), and aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans) in patients with diabetes. | 2015 | 23857867 |
| is immunesenescence a contributing factor for periodontal diseases? | current concept in periodontal diseases (pds) states that it is the host's response toward the periodontal pathogens which leads to tissue destruction and attachment loss. hence the role of immune response in the progression and resolution of pd must be considered vital. any alteration in the immune system disturbs the homeostasis of the periodontium. decline in immune system is the hallmark of aging, leading to increased susceptibility of elderly individuals to bacterial infections. the periodo ... | 2013 | 23869121 |
| evaluation of the efficacy of green tea catechins as an adjunct to scaling and root planing in the management of chronic periodontitis using pcr analysis: a clinical and microbiological study. | increased knowledge of anaerobic bacteria in the development of periodontal diseases has led to new treatment strategies aiming primarily at suppression or elimination of specific periodontal pathogens. over the last few decades, green tea has been subjected to many scientific and medical studies. | 2013 | 23869127 |
| molecular microbiological evaluation of subgingival biofilm sampling by paper point and curette. | the present clinical study aimed to investigate if there are differences in microbiological outcomes dependent on the subgingival biofilm collection method. subgingival biofilm samples were collected from the four deepest pockets (>5 mm) of 17 patients with aggressive periodontitis (agp) and 33 patients with chronic periodontitis (cp), first by paper point and thereafter by curette. samples obtained with the same method were pooled together from each patient and forwarded for molecular microbiol ... | 2014 | 23879704 |
| a periodontal pathogen treponema denticola hijacks the fusobacterium nucleatum-driven host response. | periodontitis is a polymicrobial disease that arises from the dysbiosis of the plaque biofilm. to study polymicrobial interactions with gingival epithelial cells, the oral commensal fusobacterium nucleatum and the periodontal pathogen treponema denticola were chosen due to their opposing effects on the expression of human beta-defensins (hbds) and interleukin (il)-8 in gingival epithelial cells. immortalized gingival epithelial hok-16b cells were infected with either f. nucleatum or t. denticola ... | 2013 | 23897119 |
| a phylogenomic and molecular signature based approach for characterization of the phylum spirochaetes and its major clades: proposal for a taxonomic revision of the phylum. | the spirochaetes species cause many important diseases including syphilis and lyme disease. except for their containing a distinctive endoflagella, no other molecular or biochemical characteristics are presently known that are specific for either all spirochaetes or its different families. we report detailed comparative and phylogenomic analyses of protein sequences from spirochaetes genomes to understand their evolutionary relationships and to identify molecular signatures for this group. these ... | 2013 | 23908650 |
| effect of antimicrobial photodynamic therapy on periodontally infected tooth sockets in rats. | the aim of the present study was to evaluate the antimicrobial effect of antimicrobial photodynamic therapy (apdt) in alveolar treatment of areas with induced periodontitis. thirty male wistar rats were subjected to ligature-induced periodontal disease (pd) in the first left inferior molars, while the right side molars did not receive ligatures. after 7 days of pd evolution, ligatures were removed from the left side, and the first left and right mandibular molars were extracted. afterwards, anim ... | 2013 | 23912780 |
| defensins and ll-37: a review of function in the gingival epithelium. | antimicrobial peptides represent an important aspect of the innate defense system that contributes to the control of bacterial colonization and infection. as studies have progressed it has become clear that antimicrobial peptides manifest other functions in addition to their antimicrobial effects. these functions include chemotaxis of numerous types of host cells involved in both the innate and adaptive immune responses. in this review, the antimicrobial activity, the regulation and the contribu ... | 2013 | 23931055 |
| the correlation between chronic periodontitis and oral cancer. | infections are increasingly considered as potential trigger for carcinogenesis apart from risk factors like alcohol and tobacco. the discussion about human papilloma virus (hpv) in oral squamous cell carcinoma (oscc) points at a general role of infection for the development of oral carcinomas. furthermore, first studies describe a correlation between chronic periodontitis and oscc, thus, characterizing chronic inflammation as being a possible trigger for oscc. in front of this background, we pre ... | 2013 | 23936684 |
| the association between detectable plasmatic human immunodeficiency virus (hiv) viral load and different subgingival microorganisms in brazilian adults with hiv: a multilevel analysis. | this study investigates the association between detectable plasmatic human immunodeficiency virus (hiv) viral load (hvl) and high levels of periodontal- and non-periodontal-related microorganisms in the subgingival microbiota of individuals with hiv. | 2014 | 23952074 |
| acrylyl-coenzyme a reductase, an enzyme involved in the assimilation of 3-hydroxypropionate by rhodobacter sphaeroides. | the anoxygenic phototroph rhodobacter sphaeroides uses 3-hydroxypropionate as a sole carbon source for growth. previously, we showed that the gene (rsp_1434) known as acui, which encodes a protein of the medium-chain dehydrogenase/reductase (mdr) superfamily, was involved in 3-hydroxypropionate assimilation via the reductive conversion to propionyl-coenzyme a (coa). based on these results, we speculated that acui encoded acrylyl-coa reductase. in this work, we characterize the in vitro enzyme ac ... | 2013 | 23955006 |
| characterization of three novel adhesins of leptospira interrogans. | we report cloning, expression, purification, and characterization of three predicted leptospiral membrane proteins (lic11360, lic11009, and lic11975). in silico analysis and proteinase k accessibility data suggest that these proteins might be surface exposed. we show that proteins encoded by lic11360, lic11009 and lic11975 genes interact with laminin in a dose-dependent and saturable manner. the proteins are referred to as leptospiral surface adhesions 23, 26, and 36 (lsa23, lsa26, and lsa36), r ... | 2013 | 23958908 |
| expression of human beta defensins (hbds) 1, 2 and 3 in gingival crevicular fluid of patients affected by localized aggressive periodontitis. | to study the effect of nonsurgical periodontal therapy on the expression frequencies of human beta-defensin (hbd)-1, -2, and -3 in the gingival crevicular fluid (gcf) of patients affected by localized aggressive periodontitis. | 2013 | 23960559 |
| environmental stimuli shape biofilm formation and the virulence of periodontal pathogens. | periodontitis is a common inflammatory disease affecting the tooth-supporting structures. it is initiated by bacteria growing as a biofilm at the gingival margin, and communication of the biofilms differs in health and disease. the bacterial composition of periodontitis-associated biofilms has been well documented and is under continual investigation. however, the roles of several host response and inflammation driven environmental stimuli on biofilm formation is not well understood. this review ... | 2013 | 23965982 |
| bacterial community development in experimental gingivitis. | current knowledge of the microbial composition of dental plaque in early gingivitis is based largely on microscopy and cultural methods, which do not provide a comprehensive description of oral microbial communities. this study used 454-pyrosequencing of the v1-v3 region of 16s rrna genes (approximately 500 bp), and bacterial culture, to characterize the composition of plaque during the transition from periodontal health to gingivitis. a total of 20 healthy volunteers abstained from oral hygiene ... | 2013 | 23967169 |
| genome-wide relatedness of treponema pedis, from gingiva and necrotic skin lesions of pigs, with the human oral pathogen treponema denticola. | treponema pedis and t. denticola are two genetically related species with different origins of isolation. treponema denticola is part of the human oral microbiota and is associated with periodontitis while t. pedis has been isolated from skin lesions in animals, e.g., digital dermatitis in cattle and necrotic ulcers in pigs. although multiple treponema phylotypes may exist in ulcerative lesions in pigs, t. pedis appears to be a predominant spirochete in these lesions. treponema pedis can also be ... | 2013 | 23977007 |
| decontamination of dental implant surface in peri-implantitis treatment: a literature review. | etiological treatment of peri-implantitis aims to reduce the bacterial load within the peri-implant pocket and decontaminate the implant surface in order to promote osseointegration. the aim of this literature review was to evaluate the efficacy of different methods of implant surface decontamination. a search was conducted using the pubmed (medline) database, which identified 36 articles including in vivo and in vitro studies, and reviews of different decontamination systems (chemical, mechanic ... | 2013 | 23986023 |
| porphyromonas gingivalis and treponema denticola synergistic polymicrobial biofilm development. | chronic periodontitis has a polymicrobial biofilm aetiology and interactions between key bacterial species are strongly implicated as contributing to disease progression. porphyromonas gingivalis, treponema denticola and tannerella forsythia have all been implicated as playing roles in disease progression. p. gingivalis cell-surface-located protease/adhesins, the gingipains, have been suggested to be involved in its interactions with several other bacterial species. the aims of this study were t ... | 2013 | 23990979 |
| oral microflora and periodontal disease: new technology for diagnosis in dentistry. | periodontitis is a disease that affects and destroys the tissues that support teeth. tissues damage results from a prolonged inflammatory response to an ecological shift in the composition of subgingival biofilms. three bacterial species that constitute the red complex group, porphyromonas gingivalis, tannerella forsythia, and treponema denticola, are considered the main pathogens involved in periodontitis. in the present study a real-time pcr based assay was designed to detect and quantify red ... | 2013 | 23991267 |
| periplasmic flagellar export apparatus protein, flih, is involved in post-transcriptional regulation of flab, motility and virulence of the relapsing fever spirochete borrelia hermsii. | spirochetes are bacteria characterized in part by rotating periplasmic flagella that impart their helical or flat-wave morphology and motility. while most other bacteria rely on a transcriptional cascade to regulate the expression of motility genes, spirochetes employ post-transcriptional mechanism(s) that are only partially known. in the present study, we characterize a spontaneous non-motile mutant of the relapsing fever spirochete borrelia hermsii that was straight, non-motile and deficient i ... | 2013 | 24009690 |
| the thiamine biosynthetic enzyme thic catalyzes multiple turnovers and is inhibited by s-adenosylmethionine (adomet) metabolites. | thic (4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase; ec 4.1.99.17) is a radical s-adenosylmethionine (adomet) enzyme that uses a [4fe-4s](+) cluster to reductively cleave adomet to methionine and a 5'-deoxyadenosyl radical that initiates catalysis. in plants and bacteria, thic converts the purine intermediate 5-aminoimidazole ribotide to 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate, an intermediate of thiamine pyrophosphate (coenzyme b1) biosynthesis. in this study, assay ... | 2013 | 24014032 |
| effectiveness of sub gingival irrigation of an indigenous 1% curcumin solution on clinical and microbiological parameters in chronic periodontitis patients: a pilot randomized clinical trial. | the aim of this study is to evaluate the efficacy of 1% curcumin (cu) solution and compare it with conventional irrigant i.e., 0.2% chlorhexidine (chx) gluconate and a positive control (saline) as an adjunct to thorough scaling and root planing. | 2013 | 24015007 |
| detection of putative periodontopathic bacteria in type 1 diabetic and healthy children: a comparative study. | the aim of this study was to compare and assess the risk of periodontitis due to the presence of four putative periodontopathic bacteria (porphyromonas gingivalis, treponema denticola, tannerella forsythia, and aggregatibacter actinomycetemcomitans) in type 1 diabetic and healthy children. | 2013 | 24025882 |
| functional role of oligomerization for bacterial and plant sweet sugar transporter family. | eukaryotic sugar transporters of the mfs and sweet superfamilies consist of 12 and 7 α-helical transmembrane domains (tms), respectively. structural analyses indicate that mfs transporters evolved from a series of tandem duplications of an ancestral 3-tm unit. sweets are heptahelical proteins carrying a tandem repeat of 3-tm separated by a single tm. here, we show that prokaryotes have ancestral sweet homologs with only 3-tm and that the bradyrhizobium japonicum semisweet1, like arabidopsis swee ... | 2013 | 24027245 |
| periodontal profile and presence of periodontal pathogens in young african-americans from salvador, ba, brazil. | this cross-sectional study evaluated the periodontal status and the presence of periodontopathogens in 132 young, black ethnic subjects who live in salvador/bahia-brazil and have never smoked. periodontal probing depth (ppd), clinical attachment level (cal), plaque index (pi) and gingival index (gi) were measured and analyzed by anova and wilcoxon tests (p<0.05) according to gender and age. the presence of a.actinomycetemcomitans, p.gingivalis, e.corrodens and f.nucleatum was determined by pcr a ... | 2008 | 24031206 |
| principles and applications of polymerase chain reaction in medical diagnostic fields: a review. | recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. among these methods, polymerase chain reaction (pcr) has generated great benefits and allowed scientific advancements. pcr is an excellent technique for the rapid detection of pathogens, including those difficult to culture. along with conventional pcr techniques, re ... | 2009 | 24031310 |
| microbiota associated with chronic osteomyelitis of the jaws. | chronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. the aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some brazilian patients. after clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues fro ... | 2010 | 24031586 |
| occurrence of periodontal pathogens among patients with chronic periodontitis. | the aim of the present study was to evaluate the presence of the periodontal pathogens that form the red complex (tannerella forsythia, porphyromonas gingivalis and treponema denticola) and aggregatibacter actinomycetemcomitans in patients with chronic periodontitis. the sample consisted of 29 patients with a clinical and radiographic diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology (3). samples for microbiological analysis were collected from th ... | 2012 | 24031906 |
| transcriptome analysis of encystation in entamoeba invadens. | encystation is an essential differentiation process for the completion of the life cycle of a group of intestinal protozoa including entamoeba histolytica, the causative agent of intestinal and extraintestinal amebiasis. however, regulation of gene expression during encystation is poorly understood. to comprehensively understand the process at the molecular level, the transcriptomic profiles of e. invadens, which is a related reptilian species that causes an invasive disease similar to that of e ... | 2013 | 24040350 |
| quantitative analysis of periodontal pathogens present in the saliva of geriatric subjects. | at present, information regarding periodontal disease in geriatric patients is scarce. the purpose of this study was to quantify the periodontal pathogens present in the saliva of korean geriatric patients and assess the relationship between the bacterial levels and the periodontal condition. | 2013 | 24040571 |
| periodontal treatment downregulates protease-activated receptor 2 in human gingival crevicular fluid cells. | protease-activated receptor 2 (par2) is implicated in the pathogenesis of chronic inflammatory diseases, including periodontitis; it can be activated by gingipain and produced by porphyromonas gingivalis and by neutrophil protease 3 (p3). par2 activation plays a relevant role in inflammatory processes by inducing the release of important inflammatory mediators associated with periodontal breakdown. the effects of periodontal treatment on par2 expression and its association with levels of proinfl ... | 2013 | 24042113 |
| binding properties of treponema denticola lipooligosaccharide. | the cell-surface lipooligosaccharide (los) of treponema denticola possesses several biological properties. the aim of this study was to investigate the binding properties of t. denticola los to extracellular matrix (ecm) proteins, mucosal cells, and oral bacteria. | 2013 | 24049558 |
| salivary detection of periodontopathic bacteria in fanconi's anemia patients. | fanconi's anemia (fa) is characterized by bone marrow failure and can lead to infections such as periodontal disease. the aim of this study was to compare the prevalence of four periodontopathic bacteria including aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, fusobacterium nucleatum and treponema denticola in saliva samples from children with and without fa. paraffin-stimulated saliva samples were collected from 71 children and adolescents, aged 6-18 years old. the samples wer ... | 2013 | 24055631 |
| stimulation of mmp-1 and ccl2 by nampt in pdl cells. | periodontitis is an inflammatory disease caused by pathogenic microorganisms and characterized by the destruction of the periodontium. obese individuals have an increased risk of periodontitis, and elevated circulating levels of adipokines, such as nicotinamide phosphoribosyltransferase (nampt), may be a pathomechanistic link between both diseases. the aim of this in vitro study was to examine the regulation of periodontal ligament (pdl) cells by nampt and its production under inflammatory and i ... | 2013 | 24058270 |
| orthogonal cas9 proteins for rna-guided gene regulation and editing. | the cas9 protein from the streptococcus pyogenes crispr-cas acquired immune system has been adapted for both rna-guided genome editing and gene regulation in a variety of organisms, but it can mediate only a single activity at a time within any given cell. here we characterize a set of fully orthogonal cas9 proteins and demonstrate their ability to mediate simultaneous and independently targeted gene regulation and editing in bacteria and in human cells. we find that cas9 orthologs display consi ... | 2013 | 24076762 |
| a comparative study of antimicrobial activity of proroot mta, root mta, and portland cement on actinobacillus actinomycetemcomitans. | the purpose of our study was to evaluate the antibacterial effect of proroot mta (prmta), root mta (rmta) and portland cement (pc) at their clinical concentration (70 mg/25 µl) against actinobacillus actinomycetemcomitans (aa) one of the prominent periodontal (pocket) microorganisms. | 2008 | 24082905 |
| characterizing the s-layer structure and anti-s-layer antibody recognition on intact tannerella forsythia cells by scanning probe microscopy and small angle x-ray scattering. | tannerella forsythia is among the most potent triggers of periodontal diseases, and approaches to understand underlying mechanisms are currently intensively pursued. a ~22-nm-thick, 2d crystalline surface (s-) layer that completely covers tannerella forsythia cells is crucially involved in the bacterium-host cross-talk. the s-layer is composed of two intercalating glycoproteins (tfsa-gp, tfsb-gp) that are aligned into a periodic lattice. to characterize this unique s-layer structure at the nanom ... | 2013 | 24089361 |
| salivary biomarkers: toward future clinical and diagnostic utilities. | the pursuit of timely, cost-effective, accurate, and noninvasive diagnostic methodologies is an endeavor of urgency among clinicians and scientists alike. detecting pathologies at their earliest stages can significantly affect patient discomfort, prognosis, therapeutic intervention, survival rates, and recurrence. diagnosis and monitoring often require painful invasive procedures such as biopsies and repeated blood draws, adding undue stress to an already unpleasant experience. the discovery of ... | 2013 | 24092855 |
| inter-bacterial correlations in subgingival biofilms: a large-scale survey. | although the complexity of the oral ecology and the ecological differences between health and disease are well accepted, a clear view on the dynamics in relation to disease is lacking. in this study, the prevalence and abundance of 20 key oral bacteria was assessed in health and disease and more importantly a closer look was given to the inter-bacterial relationships. | 2014 | 24102517 |
| characterization of c-s lyase from c. diphtheriae: a possible target for new antimicrobial drugs. | the emergence of antibiotic resistance in microbial pathogens requires the identification of new antibacterial drugs. the biosynthesis of methionine is an attractive target because of its central importance in cellular metabolism. moreover, most of the steps in methionine biosynthesis pathway are absent in mammals, lowering the probability of unwanted side effects. herein, detailed biochemical characterization of one enzyme required for methionine biosynthesis, a pyridoxal-5'-phosphate (plp-) de ... | 2013 | 24106714 |
| determination of periodontopathogens in patients with cri du chat syndrome. | cri du chat syndrome is a genetic alteration associated with some oral pathologies. however, it has not been described previously any clinical relationship between the periodontal disease and the syndrome. the purpose of this comparative study was to compare periodontopathogenic flora in a group with cri du chat syndrome and another without the síndrome, to assess a potential microbiological predisposition to suffer a periodontitis. | 2013 | 24121919 |
| activation of nlrp3 and aim2 inflammasomes by porphyromonas gingivalis infection. | porphyromonas gingivalis, a major periodontopathogen, is involved in the pathogenesis of periodontitis. interleukin-1β (il-1β), a proinflammatory cytokine, regulates innate immune responses and is critical for the host defense against bacterial infection. however, excessive il-1β is linked to periodontal destruction. il-1β synthesis, maturation, and secretion are tightly regulated by toll-like receptor (tlr) signaling and inflammasome activation. we found much higher levels of inflammasome compo ... | 2014 | 24126516 |
| nlrp3 inflammasome and host protection against bacterial infection. | the inflammasome is a multi-protein complex that induces maturation of inflammatory cytokines interleukin (il)-1β and il-18 through activation of caspase-1. several nucleotide binding oligomerization domain-like receptor family members, including nlrp3, recognize unique microbial and danger components and play a central role in inflammasome activation. the nlrp3 inflammasome is critical for maintenance of homeostasis against pathogenic infections. however, inflammasome activation acts as a doubl ... | 2013 | 24133343 |
| microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis. | the detection of special bacterial species in patients with periodontitis is considered to be useful for clinical diagnosis and treatment. the collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. however, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. the aim of this cross-sectional s ... | 2014 | 24144271 |
| protein-linked glycans in periodontal bacteria: prevalence and role at the immune interface. | protein modification with complex glycans is increasingly being recognized in many pathogenic and non-pathogenic bacteria, and is now thought to be central to the successful life-style of those species in their respective hosts. this review aims to convey current knowledge on the extent of protein glycosylation in periodontal pathogenic bacteria and its role in the modulation of the host immune responses. the available data show that surface glycans of periodontal bacteria orchestrate dendritic ... | 2013 | 24146665 |
| effect of nadir cd4+ t cell count on clinical measures of periodontal disease in hiv+ adults before and during immune reconstitution on haart. | the contribution of hiv-infection to periodontal disease (pd) is poorly understood. we proposed that immunological markers would be associated with improved clinical measures of pd. | 2013 | 24146949 |
| type i pyridoxal 5'-phosphate dependent enzymatic domains embedded within multimodular nonribosomal peptide synthetase and polyketide synthase assembly lines. | pyridoxal 5'-phosphate (plp)-dependent enzymes of fold type i, the most studied structural class of the plp-dependent enzyme superfamily, are known to exist as stand-alone homodimers or homotetramers. these enzymes have been found also embedded in multimodular and multidomain assembly lines involved in the biosynthesis of polyketides (pks) and nonribosomal peptides (nrps). the aim of this work is to provide a proteome-wide view of the distribution and characteristics of type i domains covalently ... | 2013 | 24148833 |
| patient-specific analysis of periodontal and peri-implant microbiomes. | periodontally involved teeth have been implicated as 'microbial reservoirs' in the etiology of peri-implant diseases. therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the degree of congruence between adjacent peri-implant and periodontal microbiomes in states of health and disease. subgingival and peri-implant biofilm samples were collected from 81 partially edentulous individuals with periodontal and peri-implant health and disease. bacterial dna wa ... | 2013 | 24158341 |
| oxantel disrupts polymicrobial biofilm development of periodontal pathogens. | bacterial pathogens commonly associated with chronic periodontitis are the spirochete treponema denticola and the gram-negative, proteolytic species porphyromonas gingivalis and tannerella forsythia. these species rely on complex anaerobic respiration of amino acids, and the anthelmintic drug oxantel has been shown to inhibit fumarate reductase (frd) activity in some pathogenic bacteria and inhibit p. gingivalis homotypic biofilm formation. here, we demonstrate that oxantel inhibited p. gingival ... | 2014 | 24165189 |
| changes in clinical and microbiological periodontal profiles relate to progression of carotid intima-media thickness: the oral infections and vascular disease epidemiology study. | no prospective studies exist on the relationship between change in periodontal clinical and microbiological status and progression of carotid atherosclerosis. | 2013 | 24166489 |
| acquisition of oral microbes and associated systemic responses of newborn nonhuman primates. | the acquisition and development of the complex oral microbiome remain ill defined. while selected species of oral bacteria have been examined in relation to their initial colonization in neonates, a more detailed understanding of the dynamics of the microbiome has been developed only in adults. the current investigation used a nonhuman primate model to document the kinetics of colonization of the oral cavities of newborns and infants by a range of oral commensals and pathogens. differences in co ... | 2014 | 24173024 |
| salivary ph: a diagnostic biomarker. | saliva contains a variety of host defense factors. it influences calculus formation and periodontal disease. different studies have been done to find exact correlation of salivary biomarkers with periodontal disease. with a multitude of biomarkers and complexities in their determination, the salivary ph may be tried to be used as a quick chairside test. the aim of this study was to analyze the ph of saliva and determine its relevance to the severity of periodontal disease. | 2013 | 24174725 |
| microbial dysbiosis in periodontitis. | periodontitis is a biofilm-associated inflammatory disease of the periodontium. this disease appears to have multiple etiologies with microbial factor contributing to initiation of the disease and immunological factor of the host propagating the disease. this review is on the concept of "microbial dysbiosis" and molecular nature of periodontitis, and the scope of traditional and emerging technologies for treating this disease. | 2013 | 24174742 |
| effect of controlled-release periochip™ on clinical and microbiological parameters in patients of chronic periodontitis. | the aim of the present study was to evaluate and compare the clinical and microbiological effectiveness of periochip™ as an adjunct to scaling and root planing (srp) with srp alone in patients with chronic periodontitis. | 2013 | 24174754 |
| metabolic proximity in the order of colonization of a microbial community. | microbial biofilms are often composed of multiple bacterial species that accumulate by adhering to a surface and to each other. biofilms can be resistant to antibiotics and physical stresses, posing unresolved challenges in the fight against infectious diseases. it has been suggested that early colonizers of certain biofilms could cause local environmental changes, favoring the aggregation of subsequent organisms. here we ask whether the enzyme content of different microbes in a well-characteriz ... | 2013 | 24204896 |
| use of nonelectrolytes reveals the channel size and oligomeric constitution of the borrelia burgdorferi p66 porin. | in the lyme disease spirochete borrelia burgdorferi, the outer membrane protein p66 is capable of pore formation with an atypical high single-channel conductance of 11 ns in 1 m kcl, which suggested that it could have a larger diameter than 'normal' gram-negative bacterial porins. we studied the diameter of the p66 channel by analyzing its single-channel conductance in black lipid bilayers in the presence of different nonelectrolytes with known hydrodynamic radii. we calculated the filling of th ... | 2013 | 24223145 |