Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| prevalence of hepatitis e virus infection in liver transplant recipients. | hepatitis e virus (hev) infection is known to run a self-limited course. recently, chronic hepatitis e has been described in several immunosuppressed patients after solid organ transplantation. the prevalence of hev infection after transplantation, however, is unknown. we studied hev parameters [hev rna, hev immunoglobulin m (igm), and hev immunoglobulin g (igg) by enzyme-linked immunosorbent assay and confirmatory immunoblotting] in a cohort of 285 adult liver transplant recipients. the most re ... | 2009 | 19790147 |
| natural course of fulminant hepatic failure: the scenario in bangladesh and the differences from the west. | fulminant hepatic failure (fhf) is a devastating complication of acute viral hepatitis, leading to death in most cases. the etiology and predictors of outcome differ according to the geographical region. this study was conducted with the aim of evaluating the etiology, complications, and outcome of fhf in bangladesh. | 2009 | 19794267 |
| investigation of a hepatitis a outbreak from shimla himachal pradesh. | hepatitis a is an enterically transmitted viral disease, highly prevalent in india and mainly presents as a paediatric sporadic disease. this study investigated an outbreak of viral hepatitis at shimla, himachal pradesh, india, during january-march 2007. | 2009 | 19797816 |
| [antibody detection of hepatitis e virus in some human population, swine and chicken in beijing in china]. | to investigate the seroprevalence of hev infection in human population, swine and chicken in beijing region. | 2009 | 19799006 |
| detection of hepatitis e virus of genotype 3 in a farm pig in kinshasa (democratic republic of the congo). | autochthonous hepatitis e is an emerging disease in industrialized countries where a growing body of data indicates that pigs represent a reservoir for hepatitis e virus (hev) of genotype 3 or 4. in africa, only hev genotypes 1 and 2 have been identified in hepatitis e outbreaks as well as in sporadic cases. we aimed to investigate whether commercial pigs in sub-saharan africa might represent an hev reservoir using molecular assays. faecal samples from 40 pigs of the pietrain race housed in a fa ... | 2010 | 19800029 |
| transmission routes and risk factors for autochthonous hepatitis e virus infection in europe: a systematic review. | increasing numbers of non-travel-associated hepatitis e virus (hev) infections have been reported in europe in recent years. our objective was to review the evidence on risk factors and transmission routes of autochthonous hev infection and hepatitis e in europe in order to develop recommendations for future research, prevention and control. a systematic literature review was performed to identify all primary reports and studies published during 1998-2008 on hepatitis e in humans and animals in ... | 2010 | 19804658 |
| heparan sulfate proteoglycans are required for cellular binding of the hepatitis e virus orf2 capsid protein and for viral infection. | the hepatitis e virus (hev), a nonenveloped rna virus, is the causative agent of hepatitis e. the mode by which hev attaches to and enters into target cells for productive infection remains unidentified. open reading frame 2 (orf2) of hev encodes its major capsid protein, porf2, which is likely to have the determinants for virus attachment and entry. using an approximately 56-kda recombinant porf2 that can self-assemble as virus-like particles, we demonstrated that cell surface heparan sulfate p ... | 2009 | 19812150 |
| current issues in the management of paediatric viral hepatitis. | viral hepatitis poses important problems for children. in preschoolers, hepatitis a virus (hav) infection frequently causes acute liver failure. vaccinating toddlers against hav in countries with high endemicity is expected to decrease mortality. hav vaccine demonstrates efficacy (comparable to immunoglobulin) as post-exposure prophylaxis. a recently developed vaccine against hepatitis e virus (hev) may benefit fetal health, because pregnant women are most prone to acute liver failure as a resul ... | 2010 | 19840256 |
| nipah virus entry can occur by macropinocytosis. | nipah virus (niv) is a zoonotic biosafety level 4 paramyxovirus that emerged recently in asia with high mortality in man. niv is a member, with hendra virus (hev), of the henipavirus genus in the paramyxoviridae family. although niv entry, like that of other paramyxoviruses, is believed to occur via ph-independent fusion with the host cell's plasma membrane we present evidence that entry can occur by an endocytic pathway. the niv receptor ephrinb2 has receptor kinase activity and we find that ep ... | 2009 | 19854459 |
| plasma and urine biomarkers in acute viral hepatitis e. | hepatitis e, caused by the hepatitis e virus (hev), is endemic to developing countries where it manifests as waterborne outbreaks and sporadic cases. though generally self-limited with a low mortality rate, some cases progress to fulminant hepatic failure (fhf) with high mortality. with no identified predictive or diagnostic markers, the events leading to disease exacerbation are not known. our aim is to use proteomic tools to identify biomarkers of acute and fulminant hepatitis e. | 2009 | 19860894 |
| population seroprevalence of hepatitis e virus antibodies in rural bangladesh. | hepatitis e virus (hev) causes a substantial burden of sporadic and epidemic disease worldwide. hev infections result in serious morbidity and mortality, especially among pregnant women, and have significant economic costs. few population-based studies have characterized the epidemiology of hev. a rural bangladeshi population was studied to determine the age- and gender- specific population seroprevalence of antibodies to hev. of 1,134 specimens tested from a representative, random population sa ... | 2009 | 19861625 |
| serological profile of sporadic acute viral hepatitis in an area of hyper-endemic hepatitis b virus infection. | located in the south western part of saudi arabia, the gizan region is largely a rural community in which hepatitis b and chronic liver disease including hepatocellular carcinoma are highly prevalent. | 2001 | 19861776 |
| hepatitis e virus infection as a cause of graft hepatitis in liver transplant recipients. | hepatitis e virus (hev) infection induces self-limiting liver disease in immunocompetent individuals. cases of chronic hepatitis e have recently been identified in organ transplant recipients. we questioned if chronic hepatitis e plays a role in graft hepatitis after liver transplantation in a low endemic area. two hundred twenty-six liver transplant recipients, 129 nontransplanted patients with chronic liver disease, and 108 healthy controls were tested for hev antibodies. hev rna was investiga ... | 2010 | 19866448 |
| hepatitis e virus-based evaluation of a virion concentration method and detection of enteric viruses in environmental samples by multiplex nested rt-pcr. | the prevalence of enteric viruses in drinking and river water samples collected from pune, india was assessed. during an outbreak of hev in a small town near pune, water samples were screened for enteric viruses. | 2010 | 19878363 |
| the use of bayesian methods for evaluating the performance of a virus-like particles-based elisa for serology of hepatitis e virus infection in swine. | in the absence of commercial kits for serological studies specifically for hepatitis e virus (hev) infection of swine, the capsid protein of a swine genotype 3 hev was expressed using a recombinant baculovirus. this antigen was used in an enzyme-linked immunosorbent assay (elisa) for detection of anti-hev antibodies. estimation of sensitivity and specificity was carried out in comparison with a commercial serological assay using sera from pigs infected experimentally with hev genotype 3, negativ ... | 2010 | 19879297 |
| determination of the 5'-terminal sequence of subgenomic rna of hepatitis e virus strains in cultured cells. | using rna preparations extracted from plc/prf/5 cells transfected with infectious genotype 3 hepatitis e virus (hev) cdna clones or inoculated with a fecal suspension containing a genotype 4 hev, the 5'-terminal sequence of a 2.2-kb subgenomic rna of genotype 3 and 4 hevs was determined. despite an insertion of t after nucleotide 5116 or an orf3-null mutation in genotype 4 hev and/or one of the genotype 3 variants, it was found that the subgenomic rna of genotype 3 and 4 hevs initiates exclusive ... | 2009 | 19885718 |
| screening of specific diagnostic peptides of swine hepatitis e virus. | swine hepatitis e virus (swhev) is a zoonotic disease that is considered a major problem in pig production and presents a threat to human health. elucidation of the major antigenic epitopes of swhev is essential for the effective control of swhev epidemics. | 2009 | 19887014 |
| combined chloroquine and ribavirin treatment does not prevent death in a hamster model of nipah and hendra virus infection. | hendra virus (hev) and nipah virus (niv) are recently emerged, closely related and highly pathogenic paramyxoviruses that cause severe disease such as encephalitis in animals and humans with fatality rates of up to 75 %. due to their high case fatality rate following human infection and because of the lack of effective vaccines or therapy, they are classified as biosafety level 4 pathogens. a recent study reported that chloroquine, an anti-malarial drug, was effective in preventing niv and hev i ... | 2010 | 19889926 |
| detection of a novel hepatitis e-like virus in faeces of wild rats using a nested broad-spectrum rt-pcr. | hepatitis e is a rare human disease in developed countries. it is caused by hepatitis e virus (hev), which is probably transmitted zoonotically to humans from domestic pigs and wild boars. multiple reports on the detection of hev-specific antibodies in rats have suggested the presence of an hev-related agent; however, infectious virus or a viral genome has not been demonstrated so far. here, a nested broad-spectrum rt-pcr protocol was developed capable of detecting different hev types including ... | 2010 | 19889929 |
| hepatitis e outbreak on cruise ship. | in 2008, acute hepatitis e infection was confirmed in 4 passengers returning to the united kingdom after a world cruise. epidemiologic investigation showed that of 789 persons who provided blood samples, 195 (25%) were seropositive, 33 (4%) had immunoglobulin [ig] m levels consistent with recent acute infection (11 of these persons were symptomatic), and 162 (21%) had igg only, consistent with past infection. passenger mean age was 68 years. most (426/789, 54%) passengers were female, yet most w ... | 2009 | 19891860 |
| [seroprevalence and risk factors of hepatitis e among pregnant women in central tunisia.] | objectives: the study was conducted to investigate the prevalence and risk factors for hepatitis e virus (hev) infection in tunisian pregnant women. methods: a total of 404 pregnant women were enrolled. data were collected through a standard questionnaire which covered sociodemographic characteristics and risk factors. blood samples were collected and were tested for hev igm and igg antibodies, igg against hepatitis a (anti-hav igg), hepatitis b virus surface antigen (hbsag) and hepatitis c viru ... | 2009 | 19896306 |
| an outbreak of hepatitis e tracked to a spring in the foothills of the himalayas, india, 2005. | in july 2005, cases of hepatitis were reported from three villages in nainital district, uttarakhand, india. we investigated this cluster to identify the source and propose recommendations. a door-to-door search for cases of acute hepatitis was carried out in the three villages. we described the outbreak by time, place and person and conducted a cohort study to identify the source of infection. in addition, sera from cases were tested. we identified 205 cases among 1238 persons (attack rate: 16% ... | 2009 | 19907960 |
| sporadic cases of acute autochthonous hepatitis e virus infection in southwest germany. | hepatitis e infection is usually a self-limiting disease and an important cause of acute hepatitis in tropical and subtropical regions where the virus is endemic. in industrialized countries, sporadic cases of acute hepatitis e virus (hev) infections have been described and the number of documented autochthonous infections seems to be increasing. we report three sporadic cases of autochthonous hepatitis e infections in southwestern germany which presented at our university hospital within two ye ... | 2010 | 19910247 |
| antibody reactivity of conformational peptide mimics of a conserved h5n1 neutralization site in different fusion proteins. | several peptide mimics of a conserved h5n1 avian influenza virus neutralization site recognized by 8h5 mab have been reported previously. in this study, the secondary and possibly higher structural orders of the peptide mimics 122 and 125 were investigated and found to be closely related to the specific binding with 8h5 mab. these two peptide mimics were fused to three different carrier proteins, and the antibody binding activities were recovered in 4 of the 11 fusion proteins. hev structural pr ... | 2010 | 19911251 |
| seroprevalence of hepatitis e virus in the uk farming population. | hepatitis e is a zoonosis that can be acquired by the consumption of contaminated food or water, or via person-to-person spread. however, little is known about the transmission of hepatitis e virus (hev) in the uk. we investigated the epidemiology of indigenous hepatitis e infection using the phls farm cohort, a sentinel group with a history of close contact with a range of domestic animals. ten of the 413 subjects tested were positive for hepatitis e igg antibodies (2.4%). seroprevalence peaked ... | 2010 | 19912601 |
| duration of hepatitis e viremia in pregnancy. | to investigate the duration of hepatitis e virus (hev) infection in pregnant and non-pregnant women with acute viral hepatitis (avh) or fulminant hepatic failure (fhf). | 2010 | 19932653 |
| surveillance of enterovirus infections in yokohama city from 2004 to 2008. | a survey of human enterovirus (hev) infections from 2004 to 2008 was conducted in yokohama city, japan. a total of 260 clinical samples in 247 patients were shown to be positive for enterovirus. among them, 25 serotypes were identified, including 3 serotypes of poliovirus (19 samples). the prevalence rates of hand-foot-and-mouth disease (hfmd), herpangina, and respiratory illness associated with nonpolio hev infections were also analyzed. seven serotypes were highly associated with hfmd or herpa ... | 2009 | 19934543 |
| prevalence of hepatitis e virus in chinese blood donors. | a point prevalence study of hepatitis e virus (hev) in chinese blood donors was conducted, and the prevalences of antibodies against hev immunoglobulin g (igg) and igm among chinese blood donors were 32.60% and 0.94%, respectively. hev viremia was 0.07%. | 2010 | 19940058 |
| detection of hepatitis e virus in slaughtered pigs in italy. | the stools of slaughtered pigs were screened for hepatitis e virus (hev). hev rna was detected in 7.3% of the samples. hev strains were characterized as genotype 3 subtype c, a cluster previously not described in italy. these findings provide evidence that slaughterhouse workers may be exposed to hev infection. | 2010 | 19943070 |
| a sabin 2-related poliovirus recombinant contains a homologous sequence of human enterovirus species c in the viral polymerase coding region. | a type 2 vaccine-related poliovirus (strain chn3024), differing from the sabin 2 strain by 0.44% in the vp1 coding region was isolated from a patient with vaccine-associated paralytic poliomyelitis. sequences downstream of nucleotide position 6735 (3d(pol) coding region) were derived from an unidentified sequence; no close match for a potential parent was found, but it could be classified into a non-polio human enteroviruses species c (hev-c) phylogeny. the virus differed antigenically from the ... | 2010 | 19946714 |
| long-term shedding of hepatitis e virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies. | pigs are presumed reservoirs for hepatitis e virus (hev) transmission to humans. to examine infection kinetics, two litters of domestic pigs (a and b, each containing 10 piglets) infected naturally with hev were studied until pigs were 6 months old. maternal igg and iga antibodies were detected in litter a piglets, but not in litter b ones. all pigs shed hev in feces when they were 30-110 days old, and 17 developed viremia at 40-100 days of age. phylogenetic analysis revealed a highly close sequ ... | 2010 | 19950246 |
| [seasonal pattern and phylogenetic analysis with human isolates of genotype-iv hepatitis e virus in swine herds, eastern china]. | to determine the seasonal prevalence of genotype-iv hepatitis e virus (hev) in swine herds in eastern china and explore the phylogenetic relationship between swine hev and human hev in the situation that zoonotic features of hev had been confirmed. | 2009 | 19950718 |
| expression of interferon-alpha and mx protein in the livers of pigs experimentally infected with swine hepatitis e virus. | expression of interferon-alpha (ifn-alpha) and mx protein in the livers of pigs experimentally infected with swine hepatitis e virus (hev) was examined immunohistochemically. five infected and five non-infected pigs were killed at 3, 7, 14, 21, 29 and 50 days post-inoculation (dpi). tissues were collected from each pig at necropsy. multifocal lymphoplasmacytic hepatitis (p=0.042) and focal hepatocellular necrosis (p=0.037) were significantly more frequent in the swine hev-infected pigs than in t ... | 2010 | 19954791 |
| rna interference inhibits hepatitis e virus mrna accumulation and protein synthesis in vitro. | hepatitis e virus (hev) is a zoonotic pathogen to which several species, including human beings, pigs and rodents, are reported to be susceptible. to date, vaccines developed against hev still need to be improved and a structural gene (orf2), which encodes a capsid protein with high sequence conservation found across hev genotypes, is a potential candidate. to exploit the possibility of using rna interference (rnai) as a strategy against hev infection, four small interference rna (sirna) duplex ... | 2010 | 19963327 |
| hepatitis e virus infection in central china reveals no evidence of cross-species transmission between human and swine in this area. | hepatitis e virus (hev) is a zoonotic pathogen of which several species of animal were reported as reservoirs. swine stands out as the major reservoir for hev infection in humans, as suggested by the close genetic relationship of swine and human virus. since 2000, genotype 4 hev has become the dominant cause of hepatitis e disease in china. recent reports showed that genotype 4 hev is freely transmitted between humans and swine in eastern and southern china. however, the infection status of hev ... | 2009 | 19997619 |
| hepatitis e in hiv-positive patients in a low-endemic country. | 2010 | 20002566 | |
| much meat, much malady: changing perceptions of the epidemiology of hepatitis e. | hepatitis e, which is caused by hepatitis e virus (hev), may now be considered a zoonosis as well as an anthroponosis. pigs, boars and deer have been identified as reservoirs, and their flesh and entrails--as meat and offal--as vehicles of hev transmission. shellfish also act as vehicles. dietary, gastronomic and culinary preferences influence how extensively hev conveyed by these vehicles can be inactivated before their ingestion by the host. another route of infection is paved by hev that is e ... | 2010 | 20002688 |
| detection of hepatitis e virus in samples of animal origin collected in hungary. | hepatitis e virus (hev) is an enterically transmitted human pathogen. hev infections are mainly associated with acute, self-limited, icteric hepatitis with an average mortality rate of 1%. animal reservoirs are considered to play an important role in the maintenance of the virus and in the spread of hev to humans. hev-induced seroconversion was described in several species, however clinical hepatitis in animals has not been observed to date. hev strains from animals are genetically closely relat ... | 2010 | 20005644 |
| serological prevalence of hepatitis e virus in domestic animals and diversity of genotype 4 hepatitis e virus in china. | pigs have been confirmed to be reservoirs of some genotypes of hepatitis e virus (hev), and other nonhuman species are also likely infected with the virus. to assess the prevalence of hev infection in domestic animals in china, 3579 serum samples, including 1967 swine, 700 goat, and 912 cattle sera, were collected from 26 provinces across the country and tested for hev antibodies and antigen using enzyme immunoassays. the results showed that 82.2% of the swine samples, but only 10.4% and 28.2% o ... | 2010 | 20021275 |
| a nationwide survey of hepatitis e virus infection in the general population of japan. | to investigate nationwide the prevalence of hepatitis e virus (hev) infection in the general population of japan, serum samples were collected from 22,027 individuals (9,686 males and 12,341 females; age, mean +/- standard deviation: 56.8 +/- 16.7 years; range: 20-108 years) who lived in 30 prefectures located in hokkaido, mainland honshu, shikoku, and kyushu of japan and underwent health check-ups during 2002-2007, and were tested for the presence of igg, igm, and iga classes of antibodies to h ... | 2010 | 20029817 |
| hepatitis e epidemic, uganda. | in october 2007, an epidemic of hepatitis e was suspected in kitgum district of northern uganda where no previous epidemics had been documented. this outbreak has progressed to become one of the largest hepatitis e outbreaks in the world. by june 2009, the epidemic had caused illness in >10,196 persons and 160 deaths. | 2010 | 20031058 |
| are recipients of solid organ transplantation a high-risk population for hepatitis e virus infection? | 2010 | 20035519 | |
| recent advances in hepatitis e virus. | hepatitis e virus (hev), the causative agent of hepatitis e, belongs to the family hepeviridae. at least four major genotypes of hev have been recognized: genotypes 1 and 2 are restricted to humans and associated with epidemics in developing countries, whereas genotypes 3 and 4 are zoonotic and infect humans and several other animals in both developing and industrialized countries. besides humans, strains of hev have been genetically identified from swine, chickens, sika deer, mongeese, and rabb ... | 2010 | 20040046 |
| general epidemiological parameters of viral hepatitis a, b, c, and e in six regions of china: a cross-sectional study in 2007. | viral hepatitis is a serious health burden worldwide. to date, few reports have addressed the prevalence of hepatitis a, b, c, and e in china. therefore, the general epidemiological parameters of viral hepatitis remain unknown. | 2009 | 20041146 |
| application of truncated immunodominant polypeptide from hepatitis e virus (hev) orf2 in an assay to exclude nonspecific binding in detecting anti-hev immunoglobulin m. | the diagnosis of recent hepatitis e virus (hev) infection depends on serologic testing for anti-hev igm; however, false-positive results may occur. in the present study, we cloned the orf2 fragment of genotype 4 hev and demonstrated that a subregion covering amino acids 459 to 607 in orf2 forms the immunodominant b-cell epitopes, as it does in genotype 1 viruses. truncation of several residues from either the n or c terminus of the polypeptide abolished the reactivity of anti-hev from naturally ... | 2010 | 20042615 |
| prevalence of hepatitis e virus-specific antibodies in sera of german domestic pigs estimated by using different assays. | hepatitis e virus is the causative agent of an acute hepatitis in humans. in industrialized countries, autochthonous hepatitis e cases in the past were mainly of undetermined origin, whereupon nowadays some cases may be linked to zoonotic transmission of hev from pigs and wild boars. in contrast to several european countries the hev status of german domestic pigs and a possible risk of transmission are unknown so far. here, a novel peptide-based elisa was used to detect hev-specific antibodies i ... | 2010 | 20045268 |
| hepatitis e virus in retail pig livers. | 2010 | 20045862 | |
| enteroviruses, type 1 diabetes and hygiene: a complex relationship. | type 1 diabetes (t1d) is an autoimmune disease in which the immune system mounts an attack on the host's insulin-producing beta cells. because most cases of t1d cannot be attributed only to individual genetics, it is strongly inferred that there is a significant environmental contribution, such as infection, impacting disease development. the human enteroviruses (hev) are common picornaviruses often implicated as triggers of human t1d, although precisely which of the numerous hev may be involved ... | 2010 | 20049905 |
| human enterovirus 71 and hand, foot and mouth disease. | hand, foot and mouth disease (hfmd) is generally a benign febrile exanthematous childhood disease caused by human enteroviruses. the route of transmission is postulated to be faeco-oral in developing areas but attributed more to respiratory droplet in developed areas. transmission is facilitated by the prolonged environmental survival of these viruses and their greater resistance to biocides. serious outbreaks with neurological and cardiopulmonary complications caused by human enterovirus 71 (he ... | 2010 | 20056019 |
| fulminant hepatitis e in a woman taking oral contraceptive medication. | we describe a fulminant autochthonous hepatic failure caused by hepatitis e (hev) in a patient admitted in our hospital for liver-transplant evaluation. the only risk factor recorded for this severe course was the use of oral contraceptives that are known to mimic a hormonal status similar to pregnancy. the diagnosis was based on the presence of igg and igm anti-hev in the serum of the patient and confirmed by the isolation of a strain of hev genotype 3f from a blood sample obtained the fourth d ... | 2010 | 20064988 |
| prevalence of occult hepatitis b virus infection in haemodialysis patients from central greece. | to assess the hepatitis b virus (hbv)-dna and the prevalence of occult hbv infection in end-stage renal failure (esrf) patients from central greece. | 2010 | 20066742 |
| ntpase and 5' to 3' rna duplex-unwinding activities of the hepatitis e virus helicase domain. | hepatitis e virus (hev) is a causative agent of acute hepatitis, and it is the sole member of the genus hepevirus in the family hepeviridae. the open reading frame 1 (orf1) protein of hev encodes nonstructural polyprotein with putative domains for methyltransferase, cysteine protease, helicase and rna-dependent rna polymerase. it is not yet known whether orf1 functions as a single protein with multiple domains or is processed to form separate functional units. on the basis of amino acid conserve ... | 2010 | 20071563 |
| [study progress on hepatitis e]. | 10%-20% of acute clinical sporatic hepatitis is hepatitis e (he). he has a severe diaease low mortality in general population of about 0.5-1.5%. it has been reported as severe disease and the mortality increased to 20% in pregnant women and old people. china is high-epidemic for hepatitis e virus (hev). hev is mainly transmitted through faecal-oral route, data also showed that hev could be transferred through blood donation. the epidemiology, diagnostics and vaccine development of he is reviewed ... | 2009 | 20077743 |
| [genotype distribution of enterovirus a species isolated in shandong province, china]. | in order to study the genotypes and molecular evolution of human enterovirus (hev) a species in shandong province, stool samples were collected from afp and hfmd patients in shandong province and virus isolation was performed. reverse transcription-polymerase chain reactions (rt-pcr) specific for ev71 and cva16 were performed with the virus isolates from hfmd patients. positive isolates were selected for entire vp1 coding gene amplification and sequencing. isolates with negative pcr results and ... | 2009 | 20077929 |
| microarray blood testing: pros & cons. | blood donation screening represents rather a unique set of blood grouping-related and pathogen detection assays. we are confronted with continuously growing numbers of testing targets. ideally, the spectrum of clinically significant blood group antigens and alloantibodies would be wider than allowed by current routine tests. at the same time, we are witnessing an increase in emerging and re-emerging human pathogens due to urbanisation, increased international travel and trade, climate change and ... | 2010 | 20079661 |
| seroprevalence of subclinical hev infection in pregnant women from north india: a hospital based study. | hepatitis e virus (hev) is a major public health problem in the developing countries. hev infection in pregnant women is more common and fatal in the third trimester. the mortality rate due to hev-induced hepatitis is as high as 15-20 per cent. the present study was designed to determine the seroprevalence of subclinical hev infection in pregnant primigravidae women. | 2009 | 20090131 |
| detection of hepatitis e virus (hev) in italian pigs displaying different pathological lesions. | in this study we investigated the hev prevalence in italian pigs displaying different pathological lesions, possible risk factors related to the infection, and the possible relations occurring between hev and other concomitant pig pathogens. genetic characterization of some of the identified strains was also performed. detection of hev rna was accomplished using a nested reverse-transcription polymerase chain reaction on bile samples from 137 pigs of 2-4months of age submitted for diagnostic pur ... | 2010 | 20092862 |
| [hepatitis e infection in dialysis and after transplantation]. | hepatitis e virus (hev) causes epidemics of acute hepatitis in developing countries, and also appears to be an emerging agent in industrialized countries. hev infection is transmitted via the fecal-oral route, and may be a zoonosis in industrialized countries. hev infection was thought to be responsible for acute hepatitis that does not become chronic. however, it has been recently reported that hev infection can evolve to chronic hepatitis and to cirrhosis, at least in solid-organ transplant pa ... | 2010 | 20096660 |
| the application of food safety interventions in primary production of beef and lamb: a review. | the production of safe red meat depends on effective control of pathogenic microorganisms at all stages of the "farm-to-fork" chain. eight microorganisms have been selected as the focus of the pathogencombat project: shiga toxin producing escherichia coli (stec), mycobacterium avium subspecies paratuberculosis (map), listeria monocytogenes, campylobacter jejuni, penicillium nordicum, invasive variants of saccharomyces cerevisiae, hepatitis e virus and tick borne encephalitis virus. the need and ... | 2010 | 20097438 |
| the entire genome sequence of hepatitis e virus genotype 3 isolated from a patient with neuralgic amyotrophy. | we report the case of a thai male patient with neuralgic amyotrophy, presenting with acute and severe pain in the upper extremities, followed by patchy muscle weakness, associated with hepatitis e virus (hev) infection. the entire hev genome was sequenced and subjected to phylogenetic analysis and found to be genotype 3. data on the most common hev genotypes circulating in thailand are scarce. the origin of infection and the mode of hev transmission in this patient remained inconclusive; however ... | 2010 | 20100114 |
| [investigation epidemiology and sub-clinical infection of hepatitis e in voluntary blood donors in south zhejiang]. | to investigate the southern region of zhejiang hepatitis e virus (hev) infection. | 2009 | 20104773 |
| [analysis of liver function index and clinical characters in 122 patients with acute hepatitis e virus coinfection with other hepatitis virus]. | to compare the liver function index and clinical characters in 122 patients with acute hepatitis e virus overlapping with other infection. | 2009 | 20104784 |
| inhibition of hepatitis e virus replication using short hairpin rna (shrna). | hepatitis e virus (hev) is a non-enveloped, single-stranded, positive sense rna virus, which is a major cause of water-borne hepatitis. rna interference (rnai) is a sequence-specific cellular antiviral defence mechanism, induced by double-stranded rna, which we used to investigate knockdown of several genes and the 3' cis-acting element (cae) of hev. in the present report, shrnas were developed against the putative helicase and replicase domains and the 3'cae region of hev. production of sirna w ... | 2010 | 20105445 |
| hepatitis e virus (hev) strains in serum samples can replicate efficiently in cultured cells despite the coexistence of hev antibodies: characterization of hev virions in blood circulation. | we recently developed a cell culture system for hepatitis e virus (hev) in plc/prf/5 and a549 cells, using fecal specimens from hev-infected patients. since transfusion-associated hepatitis e has been reported, we examined plc/prf/5 and a549 cells for the ability to support replication of hev in various serum samples obtained from 23 patients with genotype 1, 3, or 4 hev. hev progenies emerged in culture media of plc/prf/5 cells, regardless of the coexistence of hev antibodies in serum but depen ... | 2010 | 20107086 |
| pegylated interferon-alpha for treating chronic hepatitis e virus infection after liver transplantation. | this study assessed the effect of a 3-month course of pegylated interferon-alpha-2a (peg-ifn-alpha-2a) in 3 liver transplant patients with chronic active hepatitis e. a virological response was sustained for 6 and 5 months in 2 patients after peg-ifn-alpha-2a therapy was completed. a relapse was observed in the third patient. | 2010 | 20113176 |
| first report of a human autochthonous hepatitis e virus infection in brazil. | sporadic acute hepatitis e cases occurring in non-endemic areas have been associated to genotypes 3 and 4 of hepatitis e virus. several studies have demonstrated the relationship among human and animals strains, mostly pigs and deers, from respective areas characterizing zoonosis. circulation of genotype 3 of hev in brazilian swine herds have already been demonstrated. nevertheless, no confirmed human cases have been reported to date in brazil. | 2010 | 20116328 |
| the orf3 protein of hepatitis e virus delays degradation of activated growth factor receptors by interacting with cin85 and blocking formation of the cbl-cin85 complex. | hepatitis e virus (hev) causes an acute self-limiting disease that is endemic in developing countries. previous studies suggested that the orf3 protein (porf3) of hev is required for infection in vivo and is likely to modulate the host response. our previous work showed that porf3 localizes to early and recycling endosomes and causes a delay in the postinternalization trafficking of epidermal growth factor receptor (egfr) to late endosomes/lysosomes. here we report that porf3 also delays the tra ... | 2010 | 20130058 |
| co-infections due to leptospira, dengue and hepatitis e: a diagnostic challenge. | a case of mixed infection due to leptospira, dengue and hepatitis e is reported. similar clinical presentation and simultaneous transmission of these diseases during rainy season can cause substantial misdiagnosis. serological tests play a mainstay in diagnosis. early detection by appropriate serological tests and institution of therapy is crucial and life saving. | 2009 | 20130379 |
| hepatitis e virus and blood donors in germany. | 2010 | 20136793 | |
| influence of immunosuppressive therapy on the natural history of genotype 3 hepatitis-e virus infection after organ transplantation. | hepatitis-e virus (hev) infection can be responsible for chronic hepatitis in solid-organ transplant patients. | 2010 | 20145528 |
| analysis of the evolution in the circulation of hav and hev in eastern spain by testing urban sewage samples. | the aim of the study was to analyse the evolution of the prevalence of hav and hev in the population of eastern spain by analysing the viruses excreted in urban sewage. raw urban sewage samples were collected and analysed during several years using rt-pcr techniques and sequencing analysis. two limiting regions were analysed, one of them having implemented hav vaccination programs. acute symptomatic hev cases were also examined. results were compared with those from previous studies in the area ... | 2010 | 20154397 |
| experimental infection of rabbits with rabbit and genotypes 1 and 4 hepatitis e viruses. | a recent study provided evidence that farmed rabbits in china harbor a novel hepatitis e virus (hev) genotype. although the rabbit hev isolate had 77-79% nucleotide identity to the mammalian hev genotypes 1 to 4, their genomic organization is very similar. since rabbits are used widely experimentally, including as models of infection, we investigated whether they constitute an appropriate animal model for human hev infection. | 2010 | 20161794 |
| analysis of complete genome sequences of swine hepatitis e virus and possible risk factors for transmission of hev to humans in korea. | the hepatitis e virus (hev) is an emerging zoonotic agent, for which pigs are the most important reservoir. complete genome sequences of two swine hev strains, designated swkor-1 and swkor-2, were determined via rt-pcr and race-pcr. the strains contained genomes composed of 7,222- and 7,221-bp excluding the poly(a) tails, respectively. the swkor-1 and swkor-2 strains were classified into subtype 3a of genotype 3 via phylogenetic analysis. these strains formed a distinctive cluster in the phyloge ... | 2010 | 20166181 |
| experimental inoculation study indicates swine as a potential host for hendra virus. | hendra virus (hev) is a zoonotic virus from the family paramyxoviridae causing fatal disease in humans and horses. five-week-old landrace pigs and 5-month-old gottingen minipigs were inoculated with approximately 10(7) plaque forming units per animal. in addition to fever and depression exhibited in all infected pigs, one of the two landrace pigs developed respiratory signs at 5 days post-inoculation (dpi) and one of the gottingen minipigs developed respiratory signs at 5 dpi and mild neurologic ... | 2010 | 20167195 |
| evidence of person-to-person transmission of hepatitis e virus during a large outbreak in northern uganda. | outbreaks of infection with hepatitis e virus (hev) are frequently attributed to contaminated drinking water, even if direct evidence for this is lacking. | 2010 | 20178415 |
| global lymphoid tissue remodeling during a viral infection is orchestrated by a b cell-lymphotoxin-dependent pathway. | adaptive immune responses are characterized by substantial restructuring of secondary lymphoid organs. the molecular and cellular factors responsible for virus-induced lymphoid remodeling are not well known to date. here we applied optical projection tomography, a mesoscopic imaging technique, for a global analysis of the entire 3-dimensional structure of mouse peripheral lymph nodes (plns), focusing on b-cell areas and high endothelial venule (hev) networks. structural homeostasis of plns was c ... | 2010 | 20185585 |
| co-circulation of coxsackieviruses a6 and a10 in hand, foot and mouth disease outbreak in finland. | a nationwide outbreak of hand, foot and mouth disease (hfmd) occurred in finland in autumn 2008. the outbreak was untypical since a considerable number of clinically diagnosed patients were adults. furthermore, many of the patients suffered from onychomadesis several weeks after the acute phase of hfmd. | 2010 | 20189452 |
| [phylogenetic analysis of sporadic hepatitis e virus in eastern china.]. | to understand phylogenetic characteristics of sporadic hepatitis e virus (hev) in eastern china. | 2009 | 20193312 |
| [progress of research on transmission and pathogenesis of hepatitis e virus.]. | 2009 | 20193321 | |
| antituberculosis therapy-induced acute liver failure: magnitude, profile, prognosis, and predictors of outcome. | antituberculosis therapy (att)-associated acute liver failure (att-alf) is the commonest drug-induced alf in south asia. prospective studies on att-alf are lacking. the current study prospectively evaluated the magnitude, clinical course, outcome, and prognostic factors in att-alf. from january 1986 to january 2009, 1223 consecutive alf patients were evaluated: att alone was the cause in 70 (5.7%) patients. another 15 (1.2%) had att and simultaneous hepatitis virus infection. in 44 (62.8%) patie ... | 2010 | 20196116 |
| hepatitis e virus load in swine organs and tissues at slaughterhouse determined by real-time rt-pcr. | although uncommon in north america, hepatitis e virus (hev) has been identified in some industrialized countries in patients without a history of travel to hev-endemic countries. its presence is ubiquitous worldwide in swine populations. zoonotic transmission of swine hev to non human primates has been achieved experimentally and transmission of hev after ingestion of contaminated raw or undercooked meat is well documented. in canada, so far, no hev outbreak has been documented but hev genotype ... | 2010 | 20206394 |
| hepatitis e virus and pigs: a zoonotic risk in europe? | 2010 | 20207177 | |
| role of heat-shock protein 90 in hepatitis e virus capsid trafficking. | p239 is a virus-like particle constituted from hepatitis e virus (hev) recombinant proteins. it can be used as a surrogate for hev and as an investigative tool to study cellular interactions because of its ability to adsorb to and penetrate hepg2 cellular membranes. our objective was to use p239 to define the role of hev capsid proteins during the early stages of infection. pull-down and maldi-tof ms experiments identified three host-cell proteins, grp 78/bip, alpha-tubulin and heat-shock protei ... | 2010 | 20219895 |
| first case of genotype 4 human hepatitis e virus infection acquired in india. | this report describes a case of severe hepatitis in an individual returning from india which was found to be the result of infection with hev genotype 4. hev was diagnosed using a novel rt-pcr assay (with internal control) for hev rna detection/quantitation, described herein. this is the first documented report of zoonotic transmission of hev genotype 4 infection acquired in india. | 2010 | 20227909 |
| [hepatitis e virus (hev) genotype and the prevalence of anti-hev in 8 species of animals in the suburbs of beijing.]. | to investigate the prevalence of anti-hepatitis e virus (hev) and genotypes of hepatitis e virus in 8 species of animals including swine, cattle, sheep, horse, donkey, dog, chicken and duck in the suburb of beijing. | 2010 | 20302698 |
| severe thrombocytopenia associated with acute autochthonous hepatitis e. | 2010 | 20308017 | |
| [identification and genetic characterization of human enterovirus type 73, 75, and 97 strains of specie b isolated in shandong province]. | molecular typing was conducted for three human enteroviruses (hev) isolated from acute flaccid paralysis (afp) cases in shandong province, china. rnas from virus supernatants were extracted and complete vp1 genes were amplified by rt-pcr and sequenced. genotypes of these isolates were identified as hev type 73, 75 and 97, respectively by blast program. homology and phylogenetic tree analyses were performed. sequence analysis of vp1 gene showed significant variation compared with prototype strain ... | 2010 | 20329553 |
| [detection and sequences analysis of bovine hepatitis e virus rna in xinjiang autonomous region]. | reverse transcription-nested polymerase chain reaction (rt-npcr) was used to detect hev (hepatitis e virus, hev) rna in dung or anus-swab samples collected from the cows with the positive anti-hev antibodies in dairy farms in xinjiang autonomous region. 7 of 60 (11.67%) cows were positive for hev rna in one farm. 1 of 31 (3.23%) cows was positive in the other farm. pcr amplification products were cloned, sequenced and analyzed. the result showed that the homology among the 8 bovine hev orf2 189b ... | 2010 | 20329555 |
| [establishment of a fluorescent real-time quantitative rt-pcr assay for detection of genotype 4 hepatitis e virus in swine stools]. | the primers and probes for the real-time rt-pcr were designed based on the multiple sequence (swine and humans hev strains) alignments of the orf3 region of genotype 4 hev. a rapid, sensitive and stable taqman real-time rt-pcr assay was established, and its specificity and sensitivity were assessed, and comparison of the real-time rt-pcr with conventional and nested rt-pcr was performed. the results found that the crossing points showed linearly proportional to the logarithm of the input copy nu ... | 2010 | 20329556 |
| [quasispecies investigation on swine hepatitis e viruses]. | the objective of current study was to investigate the quasispecies of hepatitis e virus in swine. the partial orf2 region of hev envelope gene from four swine hev strains was amplified by rt-nested polymerase chain reaction (rt-npcr). after cloning and transformation of pcr products, 20 positive clones of each hev isolate were subject to sequencing and dna analysis. the homology among the different clones of each isolates was 96.8%-99.7%, 98.8%-99.7%, 98.8%-99.7% and 100%, respectively, while th ... | 2010 | 20329557 |
| hepatitis e virus: a zoonosis adapting to humans. | hepatitis e virus (hev) infection is gaining global attention, not only because of the increasing burden of the disease in low endemicity countries, in terms of morbidity and mortality rates, but also due to recent advances in the molecular virology and epidemiology of this emerging pathogen. hev infection spread can be described as the evolution of a zoonosis towards an established human infection. as known from other viruses, such as the human immunodeficiency virus or the influenza viruses, c ... | 2010 | 20335188 |
| a comparison of two commercially available anti-hev igg kits and a re-evaluation of anti-hev igg seroprevalence data in developed countries. | in developed countries, the incidence of hepatitis e virus (hev) infection and the resulting seroprevalence are uncertain. published estimates of seroprevalnce in these populations range from 0.26% to 31%, which may in part reflect the variety of assays used by different studies. this study compared the performance of two commercial assays (genelabs [singapore] and wantai 'beijing, china' hev igg eia kits) and reviewed published estimates of anti-hev seroprevalence in developed countries. the as ... | 2010 | 20336757 |
| hepatitis e virus-induced neurological symptoms in a kidney-transplant patient with chronic hepatitis. | it has been shown that hepatitis e virus (hev) may be responsible for chronic hepatitis in solid-organ transplant patients. it has also been suggested that hev may be responsible for atypical neurological symptoms during the acute phase. however, the relationship between the neurological symptoms and hev infection was based on the detection of anti-hev igm in the sera. herein, we report a case where neurological symptoms, that is peripheral nerve involvement with proximal muscular weakness that ... | 2010 | 20346067 |
| fate and transport of zoonotic, bacterial, viral, and parasitic pathogens during swine manure treatment, storage, and land application. | members of the public are always somewhat aware of foodborne and other zoonotic pathogens; however, recent illnesses traced to produce and the emergence of pandemic h1n1 influenza virus have increased the scrutiny on all areas of food production. the council for agricultural science and technology has recently published a comprehensive review of the fate and transport of zoonotic pathogens that can be associated with swine manure. the majority of microbes in swine manure are not zoonotic, but se ... | 2010 | 20348375 |
| viral entry inhibitors targeted to the membrane site of action. | the fusion of enveloped viruses with the host cell is driven by specialized fusion proteins to initiate infection. the "class i" fusion proteins harbor two regions, typically two heptad repeat (hr) domains, which are central to the complex conformational changes leading to fusion: the first heptad repeat (hrn) is adjacent to the fusion peptide, while the second (hrc) immediately precedes the transmembrane domain. peptides derived from the hr regions can inhibit fusion, and one hr peptide, t20 (e ... | 2010 | 20357085 |
| hepatitis e virus infection: a general review with a focus on hemodialysis and kidney transplant patients. | hepatitis e virus (hev) infection is a self-limited viral disease that causes acute hepatitis epidemics in developing countries. the common route of transmission for hev is supposedly fecal-oral. serological evidence may be unexpectedly found in hemodialysis (hd) patients and kidney transplant recipients. although the route of hev transmission is not usually determined in hd subjects, this virus seems to be transmitted either directly through hd or nosocomially. in this study, we gathered the pu ... | 2010 | 20357431 |
| zoonotic hepatitis e: animal reservoirs and emerging risks. | hepatitis e virus (hev) is responsible for enterically-transmitted acute hepatitis in humans with two distinct epidemiological patterns. in endemic regions, large waterborne epidemics with thousands of people affected have been observed, and, in contrast, in non-endemic regions, sporadic cases have been described. although contaminated water has been well documented as the source of infection in endemic regions, the modes of transmission in non-endemic regions are much less known. hev is a singl ... | 2010 | 20359452 |
| a cross-sectional epidemiological study of hbv, hcv, hdv and hev prevalence in the subcarpathian and south-eastern regions of romania. | to evaluate the prevalence of hbv, hcv, hdv and hev infections in populations with different categories of risk and the seroprevalence of hbv and hcv infections in subjects asking for a medical examination. | 2010 | 20361074 |
| treatment of chronic hepatitis e in liver transplant recipients with pegylated interferon alpha-2b. | hepatitis e virus (hev) infections are known to run a self-limiting course. recently, chronic hepatitis e has been described in immunosuppressed patients after solid-organ transplantation. besides the general recommendation to lower the immunosuppressive medication in these patients, there is currently no specific treatment. we here describe the successful use of pegylated interferon alpha-2b in the treatment of 2 liver transplant recipients who suffered a chronic hev infection for 9 years (case ... | 2010 | 20373458 |
| dimeric architecture of the hendra virus attachment glycoprotein: evidence for a conserved mode of assembly. | hendra virus is a negative-sense single-stranded rna virus within the paramyxoviridae family which, together with nipah virus, forms the henipavirus genus. infection with bat-borne hendra virus leads to a disease with high mortality rates in humans. we determined the crystal structure of the unliganded six-bladed beta-propeller domain and compared it to the previously reported structure of hendra virus attachment glycoprotein (hev-g) in complex with its cellular receptor, ephrin-b2. as observed ... | 2010 | 20375167 |