Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| fixation conditions for dna and rna in situ hybridization: a reassessment of molecular morphology dogma. | neutral buffered formalin (nbf) (4% neutral buffered formaldehyde) has been advocated by most investigators as the primary fixative of choice for in situ hybridization (ish), and specific anecdotal cautions interdicting the use of precipitating fixatives, which otherwise may offer certain advantages such as superior nuclear detail, are common. few systematic studies addressing ish fixation conditions have been published. we reasoned that heavy metals present in some precipitating fixatives may c ... | 1998 | 9422521 |
| vestibulodynia--a subset of vulvar vestibulitis or a novel syndrome? | our purpose was to recognize special features of women with both vestibulitis and constant vulvar pain (vestibulodynia) and to determine whether vestibulodynia is a separate disease entity or a subset of vestibulitis. | 1997 | 9423748 |
| destabilization of the rb tumor suppressor protein and stabilization of p53 contribute to hpv type 16 e7-induced apoptosis. | cells that express the human papillomavirus (hpv) type 16 e7 oncoprotein are predisposed to undergo apoptosis. transgenic mice that have e7 expression targeted to either the retinal photoreceptor cells or the lens cells exhibit signs of apoptosis in cells attempting to undergo differentiation. we established a cell culture system to study this process and have determined the domains of e7 that are required for predisposing cells to undergo apoptosis in response to growth arrest signals. regions ... | 1997 | 9426450 |
| expression of human papillomavirus 6 in inverted papilloma arising in a renal transplant recipient. | a 36-year-old renal transplant recipient taking cyclosporin a presented with bilateral nasal polypoid lesions involving the nasal septum and lateral nasal walls. pathologic findings from surgical excision demonstrated inverted papilloma (ip) with focal atypia and mild dysplasia. dna extracted from the tissue was tested with the polymerase chain reaction (pcr) using human papillomavirus (hpv) e6 and l1 consensus primers. this revealed amplification of the expected size fragment consistent with th ... | 1998 | 9432079 |
| inhibition of hpv-16 e6/e7 immortalization of normal keratinocytes by hairpin ribozymes. | hpv-16 e6 and e7 genes are required to efficiently immortalize a broad spectrum of cell types including cervical keratinocytes. therefore, the e6/e7 genes can be considered relevant targets for anti-cancer therapy. we produced several engineered hairpin (hp) ribozymes to specifically disrupt hpv-16 e6/e7 mrna. after extensive biochemical characterization, one anti-e6 hp ribozyme (r434) was selected for in vivo testing because of its superior catalytic capabilities. when expressed in cis, r434 ef ... | 1998 | 9448307 |
| recent advances in thin-layer cytology. | in recent years liquid-based cytology has emerged as an alternative to conventional cytopreparatory methods. in particular, the thinprep system has found broad acceptance in non-gynecologic cytopreparation. many laboratories have successfully applied this technique to body fluids (e.g. urine, pleural effusions), brushing samples (e.g. gastrointestinal tract, lung) and fine-needle aspiration. most comparative studies have shown the thinprep system to perform as well as or better than conventional ... | 1998 | 9451555 |
| the carboxyl-terminal zinc-binding domain of the human papillomavirus e7 protein can be functionally replaced by the homologous sequences of the e6 protein. | the carboxyl-terminus is necessary for the functional and structural integrity of the human papillomavirus (hpv) e7 oncoprotein. since many mutations in this domain of e7 result in the formation of unstable proteins, we have evaluated the importance of this region by replacing it with structurally related domains derived from hpv e6 proteins. biological analysis of these mutant chimeric e7/e6 proteins showed that they retained e7-specific biological activities including cooperation with the ras ... | 1997 | 9453149 |
| human papillomavirus types 52 and 58. | 1998 | 9455813 | |
| a distamycin a-inducible fragile site, fra8e, located in the region of the hereditary multiple exostoses gene, is not involved in hpv16 dna integration and amplification. | the rare fragile site is a specific point on a chromosome that is expressed as an isochromatid gap or break under certain conditions of cell culture and is inherited in a mendelian codominant fashion. five folate-sensitive fragile sites were cloned, and the molecular basis of fragile site mutation was shown to be a new class of mutation, called dynamic mutation, resulting from an allelic expansion of (ccg)n repeats. the mechanism responsible for other types of rare fragile sites, i.e., distamyci ... | 1998 | 9460496 |
| detection of hpv-16 dna by pcr in histologically cancer free lymph nodes from patients with cervical cancer. | the prognostic value of detection of human papillomavirus (hpv) type 16 dna in histologically cancer free lymph nodes was assessed in left obturator lymph nodes from cervical cancer patients with hpv-16 positive primary tumours. hpv-16 dna was detected by polymerase chain reaction in 12 of 35 patients with histologically cancer free lymph nodes. of these 12 patients, only one developed a recurrence, suggesting hpv-16 dna detection in cancer free lymph nodes has no prognostic value. | 1997 | 9462250 |
| summary of avoidable cancers in the nordic countries. | an overview is given of the most important known causes of cancer in the five nordic countries and the resulting number of cancers that are potentially avoidable. the main causes include active and passive smoking, alcohol consumption, exposure to asbestos and other occupational carcinogens, solar and ionizing radiation, obesity, human papillomavirus infection in the female genital tract and infection with helicobacter pylori. the organs most commonly affected are those of the respiratory system ... | 1997 | 9462826 |
| clinical and molecular pathology of aggressive schneiderian papilloma involving the temporal bone. | inverting papilloma is a benign but locally aggressive sinonasal tumor that rarely involves the middle ear or temporal bone. | 1998 | 9464958 |
| antibodies against early proteins of human papillomaviruses as diagnostic markers for invasive cervical cancer. | cervical cancer is the most prevalent tumor in developing countries and the second most frequent cancer among females worldwide. specific human papillomaviruses (hpvs) and, most notably, hpv types 16 and 18 are recognized as being causally associated with this malignancy. antibodies against early hpv proteins e6 and e7 have been found more often in patients with tumors than in controls. existing peptide enzyme-linked immunosorbent assays (elisas) for the detection of anti-e6 and anti-e7 antibodi ... | 1998 | 9466762 |
| retinoblastoma protein recruits histone deacetylase to repress transcription. | the retinoblastoma protein (rb) silences specific genes that are active in the s phase of the cell cycle and which are regulated by e2f transcription factors. rb binds to the activation domain of e2f and then actively represses the promoter by a mechanism that is poorly understood. here we show that rb associates with a histone deacetylase, hdac1, through the rb 'pocket' domain. association with the deacetylase is reduced by naturally occurring mutations in the pocket and by binding of the human ... | 1998 | 9468139 |
| down-regulation of human sialyltransferase gene expression during in vitro human keratinocyte cell line differentiation. | sialic acids play important roles in biological processes, such as cell-cell communication and cell-matrix interaction. histochemical analysis using pna and lfa lectin has shown that the expression of alpha 2,3-sialic acid linked to gal beta 1,3galnac is high in basal cells and decreases following further keratinocyte differentiation. in the present study, we used an in vitro keratinocyte cell line differentiation model to study expression of alpha 2,3-sialic acid linked to gal beta 1,3 galnac. ... | 1998 | 9473501 |
| [cancer of the anal canal]. | anal carcinoma is a rare malignant tumor, it occurs in only 0.02% of all malignant neoplasms. in mexico, the incidence is of 1.5%, and only 0.18% belong to the anal canal. in recent years it has been reported an increased incidence of this tumor due to the association with the human papilloma virus in hiv positive patients. the most common histological forms are the epidermoid and the cloacogenic carcinomas. the most relevant prognostic factors are the size of the tumor and the presence of lymph ... | 1997 | 9480525 |
| squamous intraepithelial lesions in cervical smears of human immunodeficiency virus-seropositive adolescents. | various authors have reported a high rate of human papillomavirus (hpv) infection and hpv-related neoplasias in human immunodeficiency virus (hiv)-seropositive women. on the other hand, young women are most susceptible to cervical infection because of immaturity of the cervix, as it appears that hpv has more access to the basal cells of the differentiating epithelium. the purpose of the present work was to study cervical smears of 82 adolescent hiv-seropositive women (13-21 years of age) to sear ... | 1998 | 9484635 |
| human fibroblasts expressing the human papillomavirus e6 gene are deficient in global genomic nucleotide excision repair and sensitive to ultraviolet irradiation. | we investigated the role of wild-type p53 activity in modulating nucleotide excision repair after uv irradiation in normal and p53-deficient primary human fibroblasts created by expression of the human papillomavirus 16 e6 gene. compared with parental cells, the e6-expressing fibroblasts were deficient in global genomic repair of both uv-induced cyclobutane pyrimidine dimers and 6-4 photoproducts but exhibited normal transcription-coupled repair. the e6-expressing cells were also more sensitive ... | 1998 | 9485006 |
| novel mechanisms of e2f induction by bk virus large-t antigen: requirement of both the prb-binding and the j domains. | e2f activity is regulated in part by the retinoblastoma family of tumor suppressor proteins. viral oncoproteins, such as simian virus 40 (sv40) large-t antigen (tag), adenovirus e1a, and human papillomavirus e7, can disrupt the regulation of cellular proliferation by binding to prb family members and dissociating e2f-prb family protein complexes. bk virus (bkv), which infects a large percentage of the human population and has been associated with a variety of human tumors, encodes a tag homologo ... | 1998 | 9488491 |
| trophoblasts are the preferential target for human papilloma virus infection in spontaneously aborted products of conception. | in a recent study it has been shown that human papillomavirus (hpv) infection was threefold more prevalent in spontaneous abortion specimens compared with elective specimens (60% [15 of 25] v 20% [3 of 15], respectively) as analyzed by broad-spectrum hpv polymerase chain reaction (pcr) amplification and dot-blot hybridization with an hpv-16 probe. in this study, archival paraffin-embedded tissue from a subset of previously analyzed spontaneous abortion cases were reanalyzed by in situ pcr amplif ... | 1998 | 9490277 |
| human papillomavirus and epstein-barr virus in epithelial carcinomas of the head and neck region. | human papillomavirus (hpv) dna has been detected in carcinomas of the upper aerodigestive tract. however, studies of the subject show considerable variation in their results, and the causal relationship between hpv and squamous cell carcinomas of the head and neck area still remains to be determined. epstein-barr virus (ebv) is consistently detected in nasopharyngeal carcinoma lesions, but little is known about its association with other carcinomas of the head and neck region. the present study ... | 1997 | 9494545 |
| failure to detect human papilloma virus in cutaneous molluscum contagiosum lesions. | in a recently published study, the coexistence of hpv and mcv was demonstrated for the first time in the same molluscum contagiosum lesions from a single patient. such a finding raises many questions in the understanding of both hpv and mcv. with this finding comes the potential of new treatments against dually infected mc lesions, which normally demonstrate high resistance to therapy. furthermore, the finding of hpv and mcv coexistence raises questions on the effects of mcv immunosuppressive ge ... | 1997 | 9494606 |
| human papillomavirus infection in esophageal carcinomas: a study of 121 lesions using multiple broad-spectrum polymerase chain reactions and literature review. | to elucidate the putative role of human papillomavirus (hpv) infection in the etiology of esophageal cancer, 121 formalin-fixed, paraffin-embedded specimens originating from a non-high-incidence area for this carcinoma, from slovenia, were screened for hpv infection using eight different polymerase chain reactions (pcr). three different hpv consensus primer sets and four primer sets specific for hpv types 6, 16, and 18 failed to detect hpv dna sequences in any of the tumor samples. fragments of ... | 1998 | 9496830 |
| [human papillomavirus as an etiological factor in cervical cancer: significance for health care practice]. | 1997 | 9499235 | |
| identification of single amino acids in the human papillomavirus 11 e2 protein critical for the transactivation or replication functions. | the papillomavirus e2 protein is required for viral transcriptional regulation and replication. the e2 protein has a modular structure with two highly conserved domains, a sequence-specific dna-binding and dimerization domain and a conserved n-terminus which is important for transcriptional transactivation, replication, and interaction with the e1 protein to determine which specific amino acids or regions in the n-terminus were important for the replication or transactivation functions. single a ... | 1998 | 9499806 |
| dna structure and flexibility in the sequence-specific binding of papillomavirus e2 proteins. | the papillomavirus e2 proteins are transcriptional regulators that bind to a consensus dna sequence accg nnnn cggt. multiple copies of this binding site are found in the viral genomes. the affinities of the naturally occurring binding sites for the e2 proteins are predominantly dependent upon the sequence of the nnnn spacer. the hierarchies of binding site affinities among the sites present in the viral genomes result in differential occupancy during the viral life-cycle. in turn, this different ... | 1998 | 9500925 |
| detection of human papillomavirus type 35 in a nodular cutaneous tumor in a patient infected with human immunodeficiency virus. | 1998 | 9502486 | |
| plasma concentrations of micronutrients during a nine-month clinical trial of beta-carotene in women with precursor cervical cancer lesions. | the effects of oral supplementation of a 30-mg dose of beta-carotene on the plasma levels of carotenoids, tocopherols, and retinol were studied sequentially in 69 patients participating in a nine-month randomized placebo controlled trial conducted to examine efficacy of beta-carotene to induce regression of cervical intraepithelial neoplasia. at each visit (baseline and 1.5, 3, 6, 9, 10.5, and 15 mo), blood samples were collected and the levels of six micronutrients were determined by high-perfo ... | 1998 | 9507512 |
| hpv 16 e7 antibody levels in cervical cancer patients: before and after treatment. | antibody response to hpv16 e7 oncoprotein may represent a marker of cervical cancer. a hpv16 gst-e7 fusion protein was used in a western blot assay to analyse the hpv16 e7 antibody response in 30 patients before and after treatment for cervical carcinoma (stage iib or iiib). patients were treated with three courses of cisplatin/bleomycin therapy followed by surgery, or with surgery alone. thirteen out of 30 patients had serum antibodies to hpv16 e7 antigen. three months after chemotherapy little ... | 1998 | 9515768 |
| interphase cytogenetic evidence for distinct genetic pathways in the development of squamous neoplasia of the uterine cervix. | human papillomavirus (hpv) infection has been implicated as an etiologic factor in most cervical cancers. however, additional genetic alterations are thought to be required for the development of a carcinogenic genotype. in the present study, interphase cytogenetics utilizing pericentromeric probes specific for chromosomes 1, 3, 11, 17, 18, and x was performed on paraffin-embedded tissue sections from 25 high-grade squamous intraepithelial lesions (sils) and 25 invasive squamous cell carcinomas ... | 1998 | 9520942 |
| detection of human papillomavirus in archival tissues. comparison of in situ hybridization and polymerase chain reaction. | formalin-fixed, paraffin-embedded tissues in pathology archives are an important resource for molecular epidemiology studies. use of these tissues requires that assays be optimized to account for inevitable variations in tissue fixation and processing that occur in the performance of routine histology. we compared results of colorimetric in situ hybridization (ish) to l1 consensus polymerase chain reaction (pcr) for detection and typing of human papillomavirus (hpv) in 180 blocks of archival tis ... | 1998 | 9524200 |
| virologic, immunologic, and clinical parameters in the incidence and progression of anal squamous intraepithelial lesions in hiv-positive and hiv-negative homosexual men. | anal cancer may be preceded by anal squamous intraepithelial lesions (asil), but the natural history of asil is poorly understood. in this report, we characterize the 2-year incidence and progression of low-grade sil (lsil) and high-grade sil (hsil) in a cohort study in 346 hiv-positive and 262 hiv-negative homosexual or bisexual men. subjects were studied at defined intervals using anal cytology, anoscopy with biopsy of visible lesions, human papillomavirus (hpv) testing, hiv serostatus, cd4 le ... | 1998 | 9525431 |
| the carboxyl-terminal region of the human papillomavirus type 16 e1 protein determines e2 protein specificity during dna replication. | the mechanism of dna replication is conserved among papillomaviruses. the virus-encoded e1 and e2 proteins collaborate to target the origin and recruit host dna replication proteins. expression vectors of e1 and e2 proteins support homologous and heterologous papillomaviral origin replication in transiently transfected cells. viral proteins from different genotypes can also collaborate, albeit with different efficiencies, indicating a certain degree of specificity in e1-e2 interactions. we repor ... | 1998 | 9525677 |
| vaginal immunization with recombinant gram-positive bacteria. | many viral and bacterial pathogens enter the body through the genital mucosa. therefore, one of the major goals of a vaccine against sexually transmitted diseases (stds) should be to induce an immune response in the genital mucosa capable of controlling the entry of the pathogen. our approach for the development of vaccines against stds is based on the use of nonpathogenic gram-positive bacteria as live vaccine vectors. | 1998 | 9526609 |
| an increased risk of cervical intra-epithelial neoplasia grade ii-iii among human papillomavirus positive patients with the hla-dqa1*0102-dqb1*0602 haplotype: a population-based case-control study of norwegian women. | several recent studies have reported different associations between hla specificities and human papillomavirus (hpv)-associated disease of the cervix. we report the distribution of dqa1 and dqb1 genes and hpv infection in a population-based case-control study including 92 patients with histologically verified cervical intraepithelial neoplasia grade ii-iii (cin ii-iii) (thus including moderate and severe dysplasia and carcinoma in situ) and 225 control subjects. we found an overrepresentation of ... | 1998 | 9533756 |
| characterization of a partially folded monomer of the dna-binding domain of human papillomavirus e2 protein obtained at high pressure. | the pressure-induced dissociation of the dimeric dna binding domain of the e2 protein of human papillomavirus (e2-dbd) is a reversible process with a kd of 5.6 x 10(-8) m at ph 5.5. the complete exposure of the intersubunit tryptophans to water, together with the concentration dependence of the pressure effect, is indicative of dissociation. dissociation is accompanied by a decrease in volume of 76 ml/mol, which corresponds to an estimated increase in solvent-exposed area of 2775 a2. there is a ... | 1998 | 9535893 |
| analysis of fhit transcripts in cervical and endometrial cancers. | carcinoma of the uterine cervix is a common malignancy, and many affected women, have been found to exhibit loss of heterozygosity (loh) in the chromosome 3p region. recent studies have localized the fhit (fragile histidine triad) gene in this region and also demonstrated a high frequency of abnormalities of this gene in various cancers. to determine the role of the fhit gene in cervical and uterine carcinomas, 16 cases of cervical carcinoma and 7 cases of endometrial carcinoma, as well as nearb ... | 1998 | 9537583 |
| human papillomavirus type 16 and risk of preinvasive and invasive vulvar cancer: results from a seroepidemiological case-control study. | 1998 | 9540960 | |
| high incidence of anal high-grade squamous intra-epithelial lesions among hiv-positive and hiv-negative homosexual and bisexual men. | the incidence of anal cancer among homosexual men exceeds that of cervical cancer in women, and hiv-positive homosexual men may be at even higher risk than hiv-negative men. cervical cancer is preceded by high-grade squamous intra-epithelial lesions (hsil) and anal hsil may similarly be the precursor to anal cancer. in this study, we describe the incidence of and risk factors for hsil in hiv-positive and hiv-negative homosexual and bisexual men. | 1998 | 9543448 |
| papillomavirus-induced genital warts in a girl--management by surgery and immunomodulating therapy. | a 4-year-old girl with condylomata acuminata of the vulva and papular warts of the surrounding skin is presented. the lesions were removed by surgery. histologic investigation showed koilocytosis of the squamous epithelium and in-situ hybridization revealed human papilloma virus type 6 infection. there were no signs of sexual abuse or sexual transmission of the virus. after ablation, an interferon-containing ointment was applied. in order to prevent recurrence, a low-molecular-weight immunomodul ... | 1998 | 9553198 |
| disruption of the e1 and e2 reading frames of hpv 16 in cervical carcinoma is associated with poor prognosis. | the e1 and e2 reading frames of 158 cervical carcinoma samples containing human papillomavirus (hpv) 16 were mapped using polymerase chain reaction (pcr). the reading frames were amplified using primers spanning the entire genes. of the analyzed samples, 23% showed no amplification with the e1 primers and 29% showed no amplification with the e2 primers. there was an overlap, but not complete identity, between the e1- and e2-disrupted groups. all e1- and e2-negative samples were further analyzed ... | 1998 | 9553812 |
| analysis of human papillomavirus infection and molecular alterations in adenocarcinoma of the cervix. | although molecular alterations involved in the development of squamous cell carcinoma of the cervix have been extensively described, these genetic changes have not been as well characterized in the development of cervical adenocarcinoma. twenty-seven paraffin-embedded adenocarcinomas of the cervix, including three cases of adenoma malignum, were analyzed for molecular alterations associated with other gynecologic malignancies. the presence of human papillomavirus (hpv) was assessed by polymerase ... | 1998 | 9556417 |
| nuclear matrix attachment regions of human papillomavirus type 16 point toward conservation of these genomic elements in all genital papillomaviruses. | the gene functions, transcriptional regulation, and genome replication of human papillomaviruses (hpvs) have been extensively studied. thus far, however, there has been little research on the organization of hpv genomes in the nuclei of infected cells. as a first step to understand how chromatin and suprachromatin structures may modulate the life cycles of these viruses, we have identified and mapped interactions of hpv dnas with the nuclear matrix. the endogenous genomes of hpv type 16 (hpv-16) ... | 1998 | 9557642 |
| imiquimod, a patient-applied immune-response modifier for treatment of external genital warts. | genital human papillomavirus infection is one of the most common sexually transmitted diseases. imiquimod is a new agent, an immune-response modifier, that has been demonstrated to have potent in vivo antiviral and antitumor effects in animal models. the present prospective, multicenter, double-blind, randomized, vehicle-controlled trial evaluated the efficacy and safety of daily patient-applied imiquimod for up to 16 weeks for the treatment of external genital warts. wart recurrence was investi ... | 1998 | 9559784 |
| presence of human papilloma virus dna in pelvic lymph nodes can predict unexpected recurrence of cervical cancer in patients with histologically negative lymph nodes. | patients without any evidence of lymph node metastases are supposed to have a fair prognosis, but some of these patients develop recurrent disease unexpectedly after surgery. the object of this study is to examine whether the detection of human papilloma virus (hpv) dna could be used as a diagnostic marker to predict such recurrences. two hundred and thirty-six patients undergoing radical hysterectomy and pelvic lymphadenectomy for stage ib and ii cervical cancer at okayama university hospital ( ... | 1998 | 9563893 |
| [perspectives for the development of vaccines and immunotherapy against cervico-uterine cancer]. | cervical cancer represents a severe public health problem and has been associated to the presence of human papillomavirus. strategies are presently being tested which target the virus to attempt to control disease progress. studies on the humoral and cell-mediated immunity of the papillomavirus infection have been useful in the development of a vaccine. synthetic virus-like particles have been validated as vaccine against several animal papillomaviruses and used to map the seroepidemiology of th ... | 1998 | 9567657 |
| type specific and genotype cross reactive b epitopes of the l1 protein of hpv16 defined by a panel of monoclonal antibodies. | mouse monoclonal antibodies (mabs) were raised against the major capsid protein, l1, of human papillomavirus type 16 (hpv16), produced in escherichia coli with the expression plasmid ptrcl1. epitope specificity could be assigned to 11 of these 12 antibodies using a series of linear peptides and fusion proteins from hpv16. one mab (mc53) recognized a novel linear epitope that appears to be unique to the hpv16 genotype. a further 11 mabs were characterized as recognizing novel and previously defin ... | 1998 | 9568027 |
| human papillomavirus and head and neck cancer: epidemiology and molecular biology. | human papillomaviruses (hpv) are known to cause cancers of the cervix and other anogenital tract sites. molecular biology has provided some evidence as to the specific mechanisms involved in the hpv-related carcinogenesis. epidemiologic and molecular biology studies have also suggested that hpv infection may be associated with cancers of the head and neck. | 1998 | 9570632 |
| prevalence, risk factors, and accuracy of cytologic screening for cervical intraepithelial neoplasia in women with the human immunodeficiency virus. | the objective was to evaluate the sensitivity and specificity of cervical cytology in women infected with the human immunodeficiency virus (hiv), risk factors for abnormal cytology in hiv-infected and uninfected women, and risk factors for histologic diagnosis of cervical intraepithelial neoplasia (cin) in hiv-infected women. | 1998 | 9570972 |
| molecular analysis of h2o2-induced senescent-like growth arrest in normal human fibroblasts: p53 and rb control g1 arrest but not cell replication. | human diploid fibroblasts lose the capacity to proliferate and enter a state termed replicative senescence after a finite number of cell divisions in culture. when treated with sub-lethal concentrations of h2o2, pre-senescent human fibroblasts enter long-term growth arrest resembling replicative senescence. to understand the molecular basis for the h2o2-induced growth arrest, we determined the cell cycle distribution, levels of p53 tumour suppressor and p21 cyclin-dependent kinase inhibitor prot ... | 1998 | 9576849 |
| dna-eia to detect high and low risk hpv genotypes in cervical lesions with e6/e7 primer mediated multiplex pcr. | oncogenicity of human papillomavirus (hpv) dna in premalignant and malignant uterine cervical diseases is mainly induced by e6/e7 open reading frame (orf). the presence of an oncogenic hpv dna may be a diagnostic marker for the detection of cytologically negative smears. | 1998 | 9577370 |
| human papillomaviruses are commonly found in normal skin of immunocompetent hosts. | we have previously demonstrated, by the combined application of two degenerate polymerase chain reaction primer sets, the presence of human papillomavirus (hpv) dna in 91% of cutaneous squamous cell cancers from renal allograft recipients, with multiple types being present in one-third of these tumors. five hpv types--hpv 20, hpv 23, hpv 38, dl40, and dl267--accounted for 73% of positive results. these hpv types are all related to the epidermodysplasia verruciformis group, and hpv 38 was origina ... | 1998 | 9579540 |
| trends in seroprevalence of human papillomavirus type 16 among pregnant women in stockholm, sweden, during 1969-1989. | to assess long-term trends in the prevalence of oncogenic human papillomavirus (hpv) infection, we performed a cross-sectional serosurvey of the seroprevalence of the major oncogenic hpv type, hpv16, among 3,512 pregnant women undergoing population-based serological screening at the first trimester of pregnancy in the same catchment area in stockholm, sweden, during 1969, 1983 or 1989. the overall hpv16 seroprevalence rates were 16% in 1969, 22% in 1983 and 21% in 1989. seroprevalence was signif ... | 1998 | 9579570 |
| intracellular expression of a single-chain antibody directed against human papillomavirus type 16 e7 oncoprotein achieves targeted antineoplastic effects. | human papillomavirus type 16 (hpv16) e7 is a viral oncoprotein that is believed to play a major role in cervical neoplasia. anti-hpv16 e7 intracellular single-chain antibodies (scfvs) were constructed to down-regulate hpv16 e7 oncoprotein in hpv dna-containing cell lines. in these studies, we transfected anti-e7 scfvs into the hpv16-positive human cervical carcinoma cell lines caski and siha and tested them for their ability to inhibit cell proliferation and alter the level of hpv16 e7 oncoprote ... | 1998 | 9581830 |
| p53-dependent signaling sustains dna replication and enhances clonogenic survival in 254 nm ultraviolet-irradiated human fibroblasts. | the cyclin-dependent kinase inhibitor p21(waf1/cip1/sdi1/cap20) exists in normal human fibroblasts in a quaternary complex with a cyclin, a cyclin-dependent kinase, and proliferating cell nuclear antigen. a model was proposed in which, during p53-mediated suppression of cell proliferation following treatment with 254 nm uv radiation (uvc), the enhanced expression of p21 might inhibit dna replication by virtue of its interactions with proliferating cell nuclear antigen. to test this model, we exa ... | 1998 | 9581844 |
| effect of retinoic acid on hpv titration and colposcopic changes in korean patients with dysplasia of the uterine cervix. | retinoids, a family of molecules capable of profound impact on many biological functions, have antiproliferative, differentiative, and immunomodulatory properties. the present study assessed the effect of 13-cis-retinoic acid (13-cra) treatment in 13 chronic cervicitis and 52 cervical intraepithelial neoplasia patients. we examined low- and high-risk human papilloma virus titer (using the hybrid capture method) and made a colposcopic and cervicographic examination before and after treatment with ... | 1997 | 9589358 |
| [study of hpv in oral squamous cell carcinoma]. | in order to demonstrate the presence of human papillomavirus (hpv) in oral squamous cell carcinoma (oscc), we detected hpv 16 and 18 type dna in oscc and normal mucosa (nom) by polymerase chain reaction (pcr), then analysed the pcr products using southern blot hybridization. the study shows that the positive rates of hpv dna were 47.8% (11/23) in oscc, including hpv16 in 6 samples, hpv18 in 3 samples and 16, 18 co-infection in 2 samples. in contrast, hpv16 dna were found to be 20% (2/10) in nom. ... | 1996 | 9592298 |
| biologic activity of oligonucleotides with polarity and anomeric center reversal. | human papillomavirus (hpv) type 16 e6 and e7 inactivate the tumor suppressors p53 and prb, respectively. both viral oncoproteins play important roles in maintaining the transformed phenotype of cells. in this study, we examine the effects of antisense oligodeoxynucleotides with polarity and anomeric center reversal (alpha/beta-odns). odns of the general structure 5'alphan3'3'nnn5'5'alphan3'3'nnnn5'5'alphan3+ ++'3'n5' were synthesized using phosphoramidite dna chemistry. these alpha/beta-odns wer ... | 1998 | 9593047 |
| detection of human papillomavirus dna in condylomata acuminata treated with co2 laser by polymerase chain reaction. | 1997 | 9594330 | |
| mice expressing the e7 oncogene of hpv16 in epithelium show central tolerance, and evidence of peripheral anergising tolerance, to e7-encoded cytotoxic t-lymphocyte epitopes. | in order to derive mice which expressed both the e7 open reading frame transgene of human papillomavirus type 16 in skin and mhc class 1 restriction elements for several e7-encoded cytotoxic t-lymphocyte (ctl) epitopes, k14.hpv16e7 mice which express e7 in basal keratinocytes were crossed to the f1 generation with a2.1 kb transgenic mice which express the mhc binding cleft domains of human hla a*0201, and murine h-2b. f1 mice (denoted k14e7 x a2.1) expressed e7 in the thymus at least as early as ... | 1998 | 9601506 |
| subungual squamous cell carcinoma in darier's disease. | we report the first case of subungual squamous cell carcinoma in a patient with darier's disease (keratosis follicularis). the presence of human papillomavirus dna was confirmed, consistent with its probable role in carcinogenesis at this site. the role of an altered immune state is discussed as an additional factor. squamous cell carcinomas may be overlooked when they occur among the other cutaneous manifestations and complications of darier's disease. a solitary, ulcerating nail bed lesion war ... | 1997 | 9604454 |
| comparison of molecular changes in lung cancers in hiv-positive and hiv-indeterminate subjects. | human immunodeficiency virus (hiv) infection has been associated with an increasing incidence of malignancy, and hiv-infected persons have an increased incidence of primary lung carcinoma compared with the general population. | 1998 | 9605900 |
| [human papillomavirus, neonatal giant cell hepatitis and biliary duct atresia]. | we previously recognized the presence of hpv-dna in cases of idiopathic neonatal giant cell hepatitis (ingch) and extrahepatic biliary duct atresia (ebda) in archivated tissue using the pcr technique. in order to investigate a possible vertical transmission we looked for the presence of hpv-dna in cervical swabs in the mothers along with formalin-fixed paraffin-embedded hepatic tissue from 3 infants with ingch and 4 patients with ebda by nested-pcr. cervical smears showed koilocytosis consistent ... | 1998 | 9607071 |
| human papillomavirus and bladder cancer. | bladder cancer remains an important cause of oncological morbidity and mortality in women. known etiological agents include smoking and exposure to certain industrial chemical compounds, though the origin of the majority of cases remains unknown. human papillomavirus infection is also common in women and has been closely linked to the development of carcinoma of the cervix. it has been suggested that infection with hpv may also be an important factor in the subsequent development of bladder canc ... | 1997 | 9609335 |
| a longitudinal study of human papillomavirus carriage in human immunodeficiency virus-infected and human immunodeficiency virus-uninfected women. | we sought to determine the relationship of human immunodeficiency virus serostatus to carriage of oncogenic human papillomavirus. | 1998 | 9609571 |
| association of human papillomavirus type 11 with carcinoma of the penis. | human papillomaviruses (hpvs) are epithelium-tropic viruses associated with several cutaneous, epithelial, and mucosal lesions. the oncogenic potential varies considerably among the more than 70 different genotypes so far identified. hpv 6 and 11 are generally found in benign genital condilomata or laryngeal papillomas, but they have been sporadically associated with genital malignancies. polymerase chain reaction (pcr) primed by degenerated consensus oligonucleotides (from a late region of the ... | 1998 | 9609652 |
| different risk factor patterns for high-grade and low-grade intraepithelial lesions on the cervix among hpv-positive and hpv-negative young women. | risk factors for cervical intraepithelial neoplasia have most often been studied in high-grade lesions. furthermore, in a high proportion of the studies, human papillomavirus (hpv), the most significant risk determinant of cervical neoplasia, was not taken into account when evaluating other risk factors. to compare risk factors for ascus (atypical cells of undetermined significance), lsil (low-grade squamous intraepithelial lesion) and hsil (high-grade squamous intraepithelial lesion), we conduc ... | 1998 | 9610715 |
| serologic profile of some sexually transmitted diseases in women with squamous intraepithelial lesions. | the purpose of this study consisted of the evaluation of some sexually transmitted diseases in patients with cervical pathology, namely squamous intraepithelial lesions. | 1998 | 9611052 |
| detection of human papillomavirus type 16 dna and of antibodies to human papillomavirus type 16 proteins in children. | we have measured markers of human papillomavirus type 16 (hpv 16) infection in children (1-10 years of age) who were hospitalized for reasons unrelated to papillomavirus infection. genital and buccal swabs obtained from 79 children were tested for the presence of hpv 16 dna by pcr. low-level positivity was found in 34 donors, twice as often in oral than in genital swabs. there was no sex-specific difference, but there was a trend towards a higher positivity rate with young age. serum antibodies ... | 1997 | 9612724 |
| differentiation-specific enhancer activity in transduced keratinocytes: a model for epidermal gene therapy. | hacat cells, a spontaneously immortalised, nontumorigenic keratinocyte line, were used as a more amenable model than primary keratinocytes for ex vivo-mediated gene transfer. these cells were transduced with retroviral vectors containing the factor ix cdna under the control of a cytomegaloviral (cmv) promoter/enhancer alone or as hybrids with either the human papilloma virus-16 (hpv-16), keratin 14 (hk14) or keratin 5 (hk5) regulatory elements. unlike primary keratinocytes, hacat cells tolerated ... | 1998 | 9614560 |
| fas ligand-mediated lysis of self bystander targets by human papillomavirus-specific cd8+ cytotoxic t lymphocytes. | mouse cytotoxic t lymphocytes (ctl) reactive with a h-2db-presented 9-mer peptide of the human papillomavirus type 16 protein e7(49-57) (rahynivtf) were generated from the spleen cells of wild-type c57bl/6 (b6) or b6 perforin-deficient (b6.p0) mice. cd8(+) b6 ctl displayed peptide-specific perforin- and fas-mediated lysis of e7-transfected mouse rma lymphoma cells (rma-e7), while cd8(+) ctl from b6.p0 mice lysed rma-e7 cells via fas ligand (fasl) exclusively. rapid and efficient lysis of syngene ... | 1998 | 9621057 |
| detection of human papillomavirus type 33 dna in extragenital bowen's disease with the polymerase chain reaction. | data on the association of human papillomavirus (hpv) infection with extragenital bowen's disease are very scarce. | 1998 | 9621134 |
| transitional cell papilloma in the fossa navicularis is positive for human papillomavirus. | transitional cell papillomas rarely develop in the fossa navicularis of the anterior urethra. we observed such a case of transitional cell papilloma and detected human papillomavirus type 6 (hpv-6) dna in this papilloma by polymerase chain reaction. to our knowledge this report is the first of hpv-infected transitional papilloma in the anterior urethra. | 1998 | 9624566 |
| bcl-2 and bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer. | it has been hypothesized that interaction of bcl-2 and bax may regulate apoptosis. the spatial and temporal interaction of bcl-2 and bax at the single cell level has not, however, been demonstrated. to achieve this goal, we have developed two-fusion fret (fluorescence resonance energy transfer). using green fluorescent protein (gfp)-bax and blue fluorescent protein (bfp)-bcl-2 fusion proteins coexpressed in the same cell, we demonstrate a direct interaction between bcl-2 and bax in individual mi ... | 1998 | 9624685 |
| p73beta, unlike p53, suppresses growth and induces apoptosis of human papillomavirus e6-expressing cancer cells. | human papillomavirus (hpv) is the major cause of cervical cancer worldwide. hpv-e6 protein targets the p53 tumor suppressor protein for degradation by ubiquitin-mediated proteolysis making such cancers resistant to p53-gene therapy. here we show that infection of human cancer cells by e6-expressing adenovirus (ad-e6) leads to degradation of both wild-type or mutant p53 protein. interestingly, the p53-homologue candidate tumor suppressor p73 is not degraded in ad-e6 infected cancer cells. wild-ty ... | 1998 | 9625796 |
| telomerase activity as a biomarker for (pre)neoplastic cervical disease in scrapings and frozen sections from patients with abnormal cervical smear. | to evaluate the diagnostic value of semi-quantitative telomerase activity assessment in cervical scrapings together with human papillomavirus (hpv) typing for detection of (pre)neoplastic cervical lesions and to compare telomerase activity in cervical scrapings and frozen specimens from the same patients. | 1998 | 9626226 |
| relationship of stable integration of herpes simplex virus-2 bg/ii n subfragment xho2 to malignant transformation of human papillomavirus-immortalized cervical keratinocytes. | transfection of the right end xho2 subfragment of bg/ii n of herpes simplex virus-2 (hsv-2) into human genital keratinocytes immortalized by human papillomavirus (hpv) type 16 or 18 resulted in invasive and noninvasive indolent cystic squamous carcinomas when cells were injected into immunocompromised mice. retention and expression of the right end portion of the bg/ii n fragment correlated with malignancy, as the corresponding hsv-2 sequences were integrated and transcribed in the tumorigenic c ... | 1998 | 9626354 |
| inactivation of p53 in a human ovarian cancer cell line increases the sensitivity to paclitaxel by inducing g2/m arrest and apoptosis. | paclitaxel-induced cytotoxicity, cell cycle perturbation, and apoptosis were determined in a human ovarian cancer cell line expressing wt p53 (a2780) and in a subclone (a2780/e6) obtained upon transfection with the product of the e6 gene of the human papilloma virus hpv16. the inactivation of wt p53 in a2780/e6 was verified by measuring the inability of the clone to induce p53 and p21 expression after paclitaxel treatment. the p53-negative clone (a2780/e6) was approximately 50-fold more sensitiv ... | 1998 | 9633517 |
| a high capacity assay for inhibitors of human papillomavirus dna replication. | the discovery of antiviral compounds against human papillomaviruses (hpv) has been hindered by the difficulties in culturing virus in vitro or assaying stable hpv dna replication. however, plasmids containing the hpv replication origin replicate transiently upon co-transfection with hpv e1 and e2 expression vectors. we have adapted this assay using secreted alkaline phosphatase (sap) as a reporter for rapid analysis of dna copy number. use of the sv40 early promoter in controlling sap expression ... | 1995 | 9636294 |
| papillomavirus and anal carcinoma. | human papilloma virus (hpv; 16 and 18) is known to play an important etiologic role in cervical dysplasia, but its relationship with anal carcinoma is still unclear. surgical samples from 80 female patients treated for anal epidermoid carcinoma in the polyclinic of surgery in geneva between 1976 and 1989 were retrospectively studied. of these, hpv detection was performed in 41 whose dna was preserved well enough to allow such an analysis. seventeen (42%) samples contained hpv, with a high percen ... | 1998 | 9638498 |
| the clinical value of digene hybrid capture hpv dna testing in a referral-based population with abnormal pap smears. | the hybrid capture human papillomavirus (hpv) dna assay is offered by the manufacturer to assist clinicians with patients with ascus pap smear results to assess the risk factor and to potentially direct follow-up of these patients. in our practice, a gynecologic oncology practice that has a referral based population with abnormal pap smears, our purpose was to evaluate the patients referred with all grades of abnormal cervical cytology. | 1998 | 9641214 |
| screening of potential semen donors for sexual transmitted diseases. | the risk of infection with sexually transmitted diseases (stds) is of great concern to couples undergoing therapeutic donor insemination. goal of study: we sought to determine the prevalence of stds in potential semen donors and assess the rate of acquisition of new infection during the follow-up period. | 1998 | 9643155 |
| human papilloma virus infection in the oral cavity--a source of infection. | 1998 | 9643338 | |
| viruses in human oral cancers. | the role of viruses in the etiology of human oral cancer is critically reviewed. available evidences show a positive correlation for human oral cancer with human papilloma virus (hpv), herpes simplex virus (hsv) and human herpes virus type-6 (hhv-6), having strongest association with hpv. these viruses may act alone or in combination with other carcinogens in the genesis of head and neck malignancies. | 1998 | 9646230 |
| the status of human papillomavirus and tumor suppressor genes p53 and p16 in carcinomas of uterine cervix from india. | infection with the high-risk strain of human papillomaviruses (hpvs) and the inactivation of the tumor suppressor gene p53 through mutation are important factors in cervical carcinogenesis. to know whether such events would occur in cervical carcinomas of indians, 43 tumors (consisting of 36 of stage iii b and 6 of stage ii b) were screened for p53 and p16 gene mutations. | 1998 | 9648588 |
| basal and human papillomavirus e6 oncoprotein-induced degradation of myc proteins by the ubiquitin pathway. | we have previously shown that the degradation of c-myc and n-myc in vitro is mediated by the ubiquitin system. however, the role of the system in targeting the myc proteins in vivo and the identity of the conjugating enzymes and possible ancillary proteins involved has remained obscure. here we report that the degradation of the myc proteins in cells is inhibited by lactacystin and mg132, two inhibitors of the 20s proteasome. inhibition is accompanied by accumulation of myc-ubiquitin conjugates. ... | 1998 | 9653139 |
| a c-rasha mutation in the metastasis of a human papillomavirus (hpv)-18 positive penile squamous cell carcinoma suggests a cooperative effect between hpv-18 and c-rasha activation in malignant progression. | human papillomaviruses (hpv) have been implicated in the etiology of anogenital squamous epithelial tumors. of the 65 hpv strains, subtypes hpv-16 and hpv-18 frequently are associated with malignant conditions and are capable of transforming keratinocytes in vitro. however, additional cellular changes are necessary to confer tumorigenicity to hpv-infected cells. secondary events implicated in the progression to malignancy include loss of tumor suppressor genes such as p53 and/or activation of ce ... | 1998 | 9655301 |
| synthetic peptides of human papillomavirus type 18 e6 harboring hla-a2.1 motif can induce peptide-specific cytotoxic t-cells from peripheral blood mononuclear cells of healthy donors. | to identify cytotoxic t-cell (ctl) epitopes against human papillomavirus type 18 (hpv 18) e6 protein that might be useful for developing peptide-based vaccine against hpv 18 infection, 18 peptides which possibly contain ctl epitopes were selected on the basis of previously described human leukocyte antigen (hla)-a2.1-binding motif and chemically synthesized. in the binding assay of the synthetic peptides, 8 out of 18 synthetic peptides enhanced the expression of hla-a2.1 molecules on t2 cell sur ... | 1998 | 9660068 |
| mutations in the tp53 gene and protein expression of p53, mdm 2 and p21/waf-1 in primary cervical carcinomas with no or low human papillomavirus load. | several studies have focused on the role of p53 inactivation in cervical cancer, either by inactivating mutations in the tp53 gene or by degradation of the p53 protein by human papillomavirus (hpv). in this study, primary cervical carcinomas from 365 patients were analysed for presence of hpv using both consensus primer-sets and type-specific primer-sets. nineteen samples were determined to have no or low virus load, and were selected for further analyses: mutation screening of the tp53 gene usi ... | 1998 | 9662253 |
| risk factors for incident and recurrent condylomata acuminata among men. a population-based study. | the rapid increase in the number of physician office visits for condylomata acuminata and the association of human papillomavirus and cancer has prompted renewed interest in the epidemiology of this sexually-transmitted disease. few epidemiologic studies have examined what risk factors are associated with condylomata acuminata in men. | 1998 | 9662760 |
| detection of human papillomavirus dna in puva-associated non-melanoma skin cancers. | psoralen and uva (puva) photochemotherapy is associated with a dose-dependent increased risk of nonmelanoma skin cancer in patients treated for psoriasis. like ultraviolet b radiation, puva is both mutagenic and immunosuppressive and may thus act as a complete carcinogen; however, the reversed squamous to basal cell carcinoma ratio (scc:bcc) in puva-treated patients, also seen in immunosuppressed renal transplant recipients, suggests a possible cofactor role for human papillomavirus (hpv) infect ... | 1998 | 9665398 |
| detection of low copy numbers of hpv dna by fluorescent in situ hybridization combined with confocal microscopy as an alternative to in situ polymerase chain reaction. | in genital lesions infected by human papillomavirus (hpv), histological criteria and hpv dna typing are of prognostic value. therefore, non-radioactive methods such as in situ hybridization are used extensively since they preserve the histological organization of the tissue, and allow the detection and characterization of hpv dna. however, the sensitivity of these methods is often limited to detection of low copy numbers of hpv dna in isolated cells or in tissue sections, and therefore alternati ... | 1998 | 9672129 |
| clinicopathologic study of basaloid squamous carcinoma of the upper aerodigestive tract. | the clinicopathologic and immunohistochemical characteristics of nine cases of basaloid squamous carcinoma (bsc) of the upper aerodigestive tract are reported, along with the results of an in situ hybridization for human papilloma virus (hpv) dna. the cases were selected through a review of 237 head and neck carcinomas, and were located in the supraglottic larynx (5), hypopharynx (2), and the base of tongue (2). the patients were 7 males and 2 females with the mean age of 62. bscs were histologi ... | 1998 | 9681804 |
| establishment of ameloblastoma cell line, am-1. | ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. the molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. we produced an immortalized ameloblastoma cell line (am-1) using human papillomavirus type-16. this cell line maintains epithelial ... | 1998 | 9682983 |
| genital infections with human papillomavirus (hpv). | 1998 | 9686734 | |
| human papillomavirus type 16 is detected in transitional cell carcinomas and squamotransitional cell carcinomas of the cervix and endometrium. | the etiologic role of human papillomavirus (hpv) in a variety of squamous neoplasms, including malignant and premalignant lesions of the cervix, is well established. the presence of hpv, predominantly hpv types 16 and 18, in adenocarcinomas of the endometrium has also been reported, although less commonly. although rare, transitional cell carcinoma (tcc) in the female genital tract, including such sites as the cervix, endometrium, and ovary, has been described. hpv, however, has not been previou ... | 1998 | 9690545 |
| risk factors for cervical neoplasia in denmark. | with the overall goal of elucidating the risk factor pattern for cervical neoplasia, two case-control studies and a prospective cohort study were conducted. the first case-control study focused on female lifestyle risk factors. it was designed to include all women (aged 20-49 years) in greater copenhagen, diagnosed with invasive cervical cancer or carcinoma in situ (cis) from january 1985 to december 1986. they were identified from the danish cancer registry. an age-stratified control group was ... | 1998 | 9693662 |
| hla class i phenotype and genotype alterations in cervical carcinomas and derivative cell lines. | downregulation of hla class i expression is a common event in tumor biology. various underlying mechanisms have been defined in different tumors, but the knowledge of hla loss mechanisms in cervical carcinoma is limited. to identify causalities for loss of surface expression, we performed a detailed investigation of hla class i phenotypes and genotypes in 5 primary cervical tumors and on derivative cell lines. protein expression on primary tissues and cell lines was evaluated by immunohistochemi ... | 1998 | 9694355 |
| hpv prevalence among mexican women with neoplastic and normal cervixes. | genital human papillomavirus (hpv) infection is causally linked to cervical cancer, yet little is known regarding hpv prevalence in cancerous and normal women in mexico, a country with a high cervical cancer incidence. we studied 185 mexican women among the patients attending gynecological outpatient clinics in four hospitals in mexico city. each woman had a pap smear, a colposcopy, and, when necessary, a biopsy. hpvs were identified by a consensus-primer-based polymerase chain reaction (pcr) as ... | 1998 | 9698486 |
| human papillomavirus genotype as a major determinant of the course of cervical cancer. | to determine whether the prognosis of invasive cancers of the uterine cervix is related to the type of human papillomavirus (hpv) associated with the tumor. | 1998 | 9704710 |