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[preparation of monoclonal antibody against pfs25 protein and establishment of double antibody sandwich elisa method].to prepare the monoclonal antibody (mab)against pfs25 protein of plasmodium falciparum, and establish the method of sandwich elisa for detecting the pfs25 protein.201122152818
simple chemiluminescence aptasensors based on resonance energy transfer.in the present work, two aptamer-based probes and related sensor systems were developed with chemiluminescence signaling. the detection was based on "turning-on" chemiluminescence with switching "off" of the resonance energy transfer after the aptamer's recognition of the target molecule. in this design, a dna/aptamer duplex linked a chemiluminescence group and a gold nanoparticle together. only low-intensity chemiluminescence was obtained due to the highly efficient resonance energy transfer. a ...201122156244
use of capillary electrophoresis with chemiluminescence detection for sensitive determination of homocysteine.a sensitive capillary electrophoresis (ce) method with chemiluminescence (cl) detection was developed for the determination of homocysteine (hcys) in human plasma. in this work, n-(4-aminobutyl)-n-ethylisoluminol was used as tagging reagent to label the analyte for achieving high assay sensitivity. n-(4-aminobutyl)-n-ethylisoluminol-tagged hcys after ce separation reacted with hydrogen peroxide in the presence of horseradish peroxidase, producing cl emission. experimental conditions for labeling ...201122162461
development of an electrochemical biosensor for the detection of aflatoxin m1 in milk.we have developed an electrochemical immunosensor for the detection of ultratrace amounts of aflatoxin m(1) (afm(1)) in food products. the sensor was based on a competitive immunoassay using horseradish peroxidase (hrp) as a tag. magnetic nanoparticles coated with antibody (anti-afm(1)) were used to separate the bound and unbound fractions. the samples containing afm(1) were incubated with a fixed amount of antibody and tracer [afm(1) linked to hrp (conjugate)] until the system reached equilibri ...201022163418
a high sensitivity micro format chemiluminescence enzyme inhibition assay for determination of hg(ii).a highly sensitive and specific enzyme inhibition assay based on alcohol oxidase (alox) and horseradish peroxidase (hrp) for determination of mercury hg(ii) in water samples has been presented. this article describes the optimization and miniaturization of an enzymatic assay using a chemiluminescence reaction. the analytical performance and detection limit for determination of hg(ii) was optimized in 96 well plates and further extended to 384 well plates with a 10-fold reduction in assay volume. ...201022163555
use of a multiplexed cmos microarray to optimize and compare oligonucleotide binding to dna probes synthesized or immobilized on individual electrodes.the combimatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined dna probes. as an alternative, we immobilized commercially synthesized dna probes on individual electrodes coated with electropolymerized polypyrrole (ppy). hybridization was measured using a biotinylated target oligonucleotide and either cy5-streptavidin and fluorescence detection or horseradish peroxidase-streptavidin and enzyme-enhanced electrochemical detection. detection efficiencies ...201022163607
enzyme-polymers conjugated to quantum-dots for sensing applications.in the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly(vinyl alcohol) (pva-cooh) to glucose oxidase (gox) followed by the bioconjugation with cds quantum-dots (qd). the resulting organic-inorganic nanohybrids were characterized by uv-visible spectroscopy, infrared spectroscopy, photoluminescence spectroscopy (pl) and transmission electron microscopy (tem). the spectroscopy results have cl ...201122163736
hollow mesoporous silica microspheres as sensitive labels for immunoassay of prostate-specific antigen.a novel electrochemical immunosensor for sensitive detection of cancer biomarker prostate-specific antigen (psa) has been reported based on nanoporous gold (npg) and hollow mesoporous silica microspheres (hsms). the immunoassay was conducted by following the typical procedure for sandwich-type immunoreaction. nanoporous gold (npg) foils were immobilized onto a glassy carbon electrode (gce) for primary anti-psa (ab(1)) anchoring. making use of the unique properties of npg, antibodies could be sel ...201222166957
chemiluminescence biosensors for dna detection using graphene oxide and a horseradish peroxidase-mimicking dnazyme.in this communication, we demonstrate that graphene oxide (go) greatly inhibits the peroxidatic activity of a horseradish peroxidase-mimicking dnazyme. combining this observation with the unique dna/go interactions, an ultrasensitive go-based chemiluminescence dna biosensing platform is developed.201222167008
specifically and reversibly immobilizing proteins/enzymes to nitriolotriacetic-acid-modified mesoporous silicas through histidine tags for purification or catalysis.six nitriolotriacetic-acid-modified ordered mesoporous silicas (nta-ompss) with different pore sizes and surface features for specific and reversible protein immobilization were fabricated and characterized. specific immobilization of a genetically engineered undecaprenyl pyrophosphate synthase (upps) from cell lysate and a chemically modified his-tagged horseradish peroxidase (hrp) in these ni-nta-ompss through histidine coordination to the nickelated nta was demonstrated and confirmed by matri ...201122167883
anionic peroxidase production by arnebia euchroma callus.arnebia euchroma callus, obtained from the root cell culture of an iranian native specimen, has gained a doubling time of 63 h after regular subculturing on linsmaier-skoog (ls) medium containing sugar (50 g/l), 2,4-dichlorophenoxyacetic acid (10(-6) m), and kinetin (10(-5) m) under darkness at 25°c. despite the observed somaclonal variations, peroxidase production by the a. euchroma calli has been stable over 4 years under the aforementioned conditions. isoelectric focusing experiments revealed ...201322172108
a sandwich electrochemical immunosensor using magnetic dna nanoprobes for carcinoembryonic antigen.a novel magnetic nanoparticle-based electrochemical immunoassay of carcinoembryonic antigen (cea) was designed as a model using cea antibody-functionalized magnetic beads [dna/fe(3)o(4)/zro(2); fe(3)o(4) (core)/zro(2) (shell) nano particles (zmps)] as immunosensing probes. to design the immunoassay, the cea antibody and o-phenylenediamine (opd) were initially immobilized on a chitosan/nano gold composite membrane on a glassy carbon electrode (gce/cs-nano au), which was used for cea recognition. ...201122174606
hydroxyl radical-induced oxidation of a phenolic c-linked 2'-deoxyguanosine adduct yields a reactive catechol.phenolic toxins stimulate oxidative stress and generate c-linked adducts at the c8-site of 2'-deoxyguanosine (dg). we previously reported that the c-linked adduct 8-(4″-hydroxyphenyl)-dg (p-phoh-dg) undergoes oxidation in the presence of na(2)ircl(6) or horseradish peroxidase (hrp)/h(2)o(2) to generate polymeric adducts through phenoxyl radical production [ weishar ( 2008 ) org. lett. 10 , 1839 - 1842 ]. we now report on reaction of p-phoh-dg with two radical-generating systems, cu(ii)/h(2)o(2) ...201222176608
influence of different length linker containing dhea-7-cmo-enzyme conjugates on sensitivity and specificity of dhea-3-hs-antibody.the introduction of spacers in coating steroid antigen or enzyme conjugates or immunogen is known to exert an influence on the sensitivity of steroid enzyme immunoassays. we have introduced different homobifunctional spacers having varying atomic length (3 to 10) between enzyme and dehydroepiandrosterone (dhea) moiety and studied their effects on functional parameters such as sensitivity and specificity of dhea enzyme immunoassays. dhea-3-hemisuccinate-bovine serum albumin (dhea-3-hs-bsa) was us ...201222181816
phenol conversion and dimeric intermediates in horseradish peroxidase-catalyzed phenol removal from water. 199422191756
a multivariate approach to structural heterogeneity of retinal ganglion cells.knowing neuronal types is essential for understanding the structural and functional organization of the nervous system. it has long been recognized that neuronal types should be discovered and not defined. this can be done using cluster analysis (ca). despite there being many studies using ca to classify neurons, only a few of them meet its formal prerequisites. in the present study, we provide an example of using ca in combination with other multivariate techniques for examining neuronal divers ...201122192506
A theory for water and macromolecular transport in the pulmo-nary artery with a detailed compariuson to the aorta.The pulmonary artery (PA) wall, which has much higher hydraulic conductivity and albumin void space and ~1/6 the normal transmural pressure of systemic arteries (e.g, aorta, ca¬rotid arteries), is rarely atherosclerotic, except under pulmonary hypertension. This paper constructs a detailed, two-dimensional, wall-structure-based filtration and macromolecular transport model for the PA in order to investigate differences in prelesion transport processes between the disease-susceptible aorta and th ...201122198178
intravenous immunoglobulins prevent the breakdown of the blood-brain barrier in experimentally induced sepsis.interventions:: the effects of immunoglobulin g and immunoglobulins enriched with immunoglobulin a and immunoglobulin m on blood-brain barrier integrity and survival rates in septic rats were comparatively investigated. measurements:: sepsis was induced by cecal ligation and perforation in sprague-dawley rats. the animals were divided into the following groups: sham, cecal ligation and perforation, cecal ligation and perforation plus immunoglobulin g (250 mg/kg, intravenous), and cecal ligation ...201122202704
hydrogen peroxide detection at a horseradish peroxidase biosensor with a au nanoparticle-dotted titanate nanotube|hydrophobic ionic liquid scaffold.in this work, a novel sensing scaffold, consisting au nanoparticle (gnp)-dotted tio(2) nanotubes (tnts) as the rigid material and the hydrophobic ionic liquid (hil), 1-decyl-3-methylimidazolium tetrafluoroborate, as the entrapping agent, was applied to facilitate the electron transfer of horseradish peroxidase (hrp) on a glassy carbon electrode. gnps were immobilised on the tnts in our work using a one-step reduction of haucl(4)·3h(2)o by sodium borohydride in the presence of sodium citrate as a ...201222204779
development of an antigen-capture enzyme-linked immunosorbent assay using monoclonal antibodies for detecting h6 avian influenza viruses.the h6 subtype of avian influenza virus (aiv) infection occurs frequently in wild and domestic birds. aiv antigen detection is preferred for controlling aiv as birds are infected before they produce antibodies. the purpose of this study was to develop an early diagnostic method for aiv detection. six monoclonal antibodies (mabs) developed from a field h6n1 aiv strain were tested for their ability to bind to viruses. the two that showed the greatest binding ability to aivs were used for antigen d ...201122206824
Differences in extrinsic innervation patterns of the small intestine in the cattle and sheep.After oral challenge of the pathological prion protein, the pathogen was first detected in the distal ileum and then deposited in the brain. The present study aims determining the possible neuronal transport pathways from the small intestine to the brain in the cattle and sheep using a tracer protein. After horseradish peroxidase was injected into the wall in the duodenum of the calf and lamb and in the ileum of the lamb, the greater part of labeled neurons was detected in the celiac and cranial ...201122209656
sensitive chemiluminescent imaging for chemoselective analysis of glycan expression on living cells using a multifunctional nanoprobe.a novel sensitive chemiluminescent (cl) imaging method was developed for in situ monitoring of cell surface glycan expression through chemoselective labeling of carbohydrate motifs and then binding to a multifunctional nanoprobe. the nanoprobe was fabricated by assembling biotin-dna and a large amount of horseradish peroxidase (hrp) on gold nanoparticles (aunps). the chemoselective labeling was performed by selective oxidization of the hydroxyl sites of sialyl and galactosyl groups on cell surfa ...201222217325
human isolates of cronobacter sakazakii bind efficiently to intestinal epithelial cells in vitro to induce monolayer permeability and apoptosis.cronobacter sakazakii (cs) is an emerging opportunistic pathogen that causes life-threatening infections in infants. this pathogen has been implicated in the outbreaks of necrotizing enterocolitis (nec) with associated rates of high mortality and morbidity. in this study, we compared the abilities of cs strains isolated from human and environmental sources to bind to intestinal epithelial cells and trigger apoptosis.201122221600
aptamer-dnazyme hairpins for biosensing of ochratoxin a.we report an aptasensor for biosensing of ochratoxin a (ota) using aptamer-dnazyme hairpin as biorecognition element. the structure of this engineered nucleic acid includes the horseradish peroxidase (hrp)-mimicking dnazyme and the ota specific aptamer sequences. a blocking tail captures a part of these sequences in the stem region of the hairpin. in the presence of ota, the hairpin is opened due to the formation of the aptamer-analyte complex. as a result, self-assembly of the active hrp-mimick ...201222221796
sensitive electrochemical immunosensor for α-synuclein based on dual signal amplification using pamam dendrimer-encapsulated au and enhanced gold nanoparticle labels.a novel electrochemical immunosensor for sensitive detection of α-synuclein (α-syn), a very important neuronal protein, has been developed based on dual signal amplification strategy. herein, g4-polyamidoamine dendrimer-encapsulated au nanoparticles (pamam-au nanocomposites) were covalently bound on the poly-o-aminobenzoic acid (poly-o-aba), which was initially electropolymerized on the electrode surface to perform abundant carboxyl groups. the formed immunosensor platform, pamam-au, was proved ...201222221797
folic acid improves inner ear vascularization in hyperhomocysteinemic mice.more than 29 million adults in the united states have been diagnosed with hearing loss. interestingly, elevated homocysteine (hcy) levels, known as hyperhomocysteinemia (hhcy), are also associated with impaired hearing. however, the associated mechanism remains obscure. the collagen receptor such as discoidin domain receptor 1 and matrix metalloproteinase (mmp) play a significant role in inner ear structure and function. we hypothesize that hhcy increases hearing thresholds by compromise in inne ...201122222235
model study of the enzymatic modification of natural extracts: peroxidase-based removal of eugenol from rose essential oil.a protocol based on the use of horseradish peroxidase (hrp) is proposed for the removal of allergenic eugenol from rose essential oil without loss of the organoleptic quality and with a good conservation of the chemical composition. for the first time, an enzyme-based strategy is proposed for essential oils treatment and opens new opportunities in the detoxification of natural extracts used in perfumery and cosmetics. our results on eugenol in rose essential oil constitute a first step toward th ...201222224510
two-step oxidation of glycerol to glyceric acid catalyzed by the phanerochaete chrysosporium glyoxal oxidase.glyoxal oxidase of p. chrysosporium is a radical copper oxidase that catalyzes oxidation of aldehydes to carboxylic acids coupled to dioxygen reduction to h(2)o(2). in addition to known substrates, glycerol is also found to be a substrate for glyoxal oxidase. during enzyme turnover, glyoxal oxidase undergoes a reversible inactivation, probably caused by loss of the active site free radical, resulting in short-lasting enzyme activities and undetectable substrate conversions. enzyme activity could ...201222226201
enhanced enzyme activity through electron transfer between single-walled carbon nanotubes and horseradish peroxidase.better understanding of electron transfer (et) taking place at the nano-bio interface can guide design of more effective functional materials used in fuel cells, biosensors, and medical devices. single-walled carbon nanotube (swcnt) coupled with biological enzymes serves as a model system for studying the et mechanism, as demonstrated in the present study. swcnt enhanced the activity of horseradish peroxidase (hrp) in the solution-based redox reaction by binding to hrp at a site proximate to the ...201222228910
fe₃o₄/au magnetic nanoparticle amplification strategies for ultrasensitive electrochemical immunoassay of alfa-fetoprotein.the purpose of this study was to devise a novel electrochemical immunosensor for ultrasensitive detection of alfa-fetoprotein based on fe(3)o(4)/au nanoparticles as a carrier using a multienzyme amplification strategy.201122228994
a new dual immunoassay for tumor markers based on chemiluminescence signal amplification by magnetic mesoporous silica and enzyme modified gold nanoparticles.a sensitive dual immunoassay was proposed for the determination of carcinoembryonic antigen (cea) and α-fetoprotein (afp) based on signal amplification. monoclonal antibodies immobilized on magnetic mesoporous silica particles (fe(3)o(4)/sio(2)) were prepared as the primary probe. horseradish peroxidase (hrp) labeled antibodies co-coated with hrp on gold nanoparticles (aunps) were used as the secondary probe to achieve signal amplification. hrp tags were retained in the flow cells after a sandwi ...201222232219
rational design of surface/interface chemistry for quantitative in vivo monitoring of brain chemistry.to understand the molecular basis of brain functions, researchers would like to be able to quantitatively monitor the levels of neurochemicals in the extracellular fluid in vivo. however, the chemical and physiological complexity of the central nervous system (cns) presents challenges for the development of these analytical methods. this account describes the rational design and careful construction of electrodes and nanoparticles with specific surface/interface chemistry for quantitative in viv ...201222236096
ph-, sugar-, and temperature-sensitive electrochemical switch amplified by enzymatic reaction and controlled by logic gates based on semi-interpenetrating polymer networks.phenylboronic acid (pba) moieties are grafted onto the backbone of poly(acrylic acid) (paa), forming the paa-pba polyelectrolyte. the semi-interpenetrating polymer network (semi-ipn) films composed of paa-pba and poly(n,n-diethylacrylamide) (pdea) were then synthesized on electrode surface with entrapped horseradish peroxidase (hrp), designated as pdea-(paa-pba)-hrp. the films demonstrated reversible ph-, fructose-, and thermo-responsive on-off behavior toward electroactive probe k(3)fe(cn)(6) i ...201222239642
dna-programmed modular assembly of cyclic and linear nanoarrays for the synthesis of two-dimensional conducting polymers.nanometer-scale arrays of conducting polymers were prepared on scaffolds of self-assembling dna modules. a series of dna oligomers was prepared, each containing six 2,5-bis(2-thienyl)pyrrole (sns) monomer units linked covalently to n4 atoms of alternating cytosines placed between leading and trailing 12-nucleobase recognition sequences. these dna modules were encoded so the recognition sequences would uniquely associate through watson-crick assembly to form closed-cycle or linear arrays of align ...201222242713
amplified detection of dna through the enzyme-free autonomous assembly of hemin/g-quadruplex dnazyme nanowires.an enzyme-free amplified detection platform is described using the horseradish peroxidase (hrp)-mimicking dnazyme as an amplifying label. two hairpin structures that include three-fourths and one-fourth of the hrp-mimicking dnazyme in caged, inactive configurations are used as functional elements for the amplified detection of the target dna. in the presence of the analyte dna, one of the hairpins is opened, and this triggers the autonomous cross-opening of the two hairpins using the strand disp ...201222242838
electron transfer and electrocatalytics of cytochrome c and horseradish peroxidase on dna modified electrode.a bio-interphase composed of dna, cytochrome c (cyt c) and horseradish peroxidase (hrp) was developed by layer-by-layer assembling cyt c, dna and cyt c-hrp on biocompatible 11-mercaptoundecanoic acid--6-mercapto-1-hexanol modified gold electrode. the new bio-interphase was used as a model system to mimic the electron transfer and electrocatalytic performance of two proteins in living organisms. results showed that the electron transfer rate at bi-protein bio-interphase was faster than those at t ...201222243789
electrochemical genosensor array for the simultaneous detection of multiple high-risk human papillomavirus sequences in clinical samples.an electrochemical genosensor array for the simultaneous detection of three high-risk human papillomavirus (hpv) dna sequences, hpv16, 18 and 45, exhibiting high sensitivity and selectivity is presented. the electrodes of a 4×4 array were modified via co-immobilization of a 1:100 (mol/mol) mixture of a thiolated probe and an oligoethyleneglycol-terminated bipodal thiol. detection of synthetic and pcr products was carried out in a sandwich type format, with the target hybridized between a surface ...201222244172
magnetic nanocomposite of anti-human igg/cooh-multiwalled carbon nanotubes/fe₃o₄ as a platform for electrochemical immunoassay.an electrochemical immunosensing method was developed based on a magnetic nanocomposite. the multiwalled carbon nanotubes (mwcnts) were treated with nitric acid to produce carboxyl groups at the open ends. then, fe₃o₄ nanoparticles were deposited on cooh-mwcnts by chemical coprecipitation of fe²⁺ and fe³⁺ salts in an alkaline solution. goat anti-human igg (anti-higg) was covalently attached to magnetic nanocomposite through amide bond formation between the carboxylic groups of mwcnts and the ami ...201222245258
a validated hplc method with fluorescence detection for the glucuronides of combretastatin a1 in human plasma, and studies on their cis-trans isomerisation.two monoglucuronides (ca1g1 and ca1g2) of the catecholic cis-stilbene combretastatin a1 (ca1, oxi4500), have been identified in a clinical trial of the bisphosphate prodrug of oxi4500, oxi4503. a validated assay for the two glucuronides in human plasma using hplc with fluorescence detection after post-column photolysis is described. the assay was linear over the range 25nm (ca1g1) or 50nm (ca1g2) - 5000nm, r(2)≥0.996. the intra-day precision for ca1g1 was better than 8.7% rsd (19.4% at the lloq) ...201122251460
detection of single-nucleotide polymorphisms with novel leaky surface acoustic wave biosensors, dna ligation and enzymatic signal amplification.this manuscript describes a new technique for detecting single-nucleotide polymorphisms (snps) by integrating a leaky surface acoustic wave (lsaw) biosensor, enzymatic dna ligation and enzymatic signal amplification. in this technique, the dna target is hybridized with a capture probe immobilized on the surface of a lsaw biosensor. then, the hybridized sequence is ligated to biotinylated allele-specific detection probe using taq dna ligase. the ligation does not take place if there is a single-n ...201122257736
synthesis and characterizations of <i>in situ</i> cross-linkable gelatin and 4-arm-ppo-peo hybrid hydrogels via enzymatic reaction for tissue regenerative medicine.<i>in situ</i> cross-linkable hybrid hydrogels composed of gelatin and 4-arm-polypropylene oxide-polyethylene oxide (tetronic) was developed as an injectable scaffold for tissue regeneration. the gelatin was modified by hydroxyphenyl propionic acid (hpa) and the tetronic was conjugated with tyramines (tet-ta). the hydrogels were rapidly formed by mixing the polymer solutions containing horseradish peroxidase (hrp) and hydrogen peroxide (h<sub>2</sub>o<sub>2</sub>). the gelation time and mecha ...201222263670
ultrasensitive electrochemical immunosensor based on au nanoparticles dotted carbon nanotube-graphene composite and functionalized mesoporous materials.a facile and sensitive electrochemical immunosensor for detection of human chorionic gonadotrophin (hcg) was designed by using functionalized mesoporous nanoparticles as bionanolabels. to construct high-performance electrochemical immunosensor, au nanoparticles (aunps) dotted carbon nanotubes (mwcnts)-graphene composite was immobilized on the working electrode, which can increase the surface area to capture a large amount of primary antibodies (ab(1)) as well as improve the electronic transmissi ...201222265320
evaluation of seven cosubstrates in the quantification of horseradish peroxidase enzyme by square wave voltammetry.the electrochemical detection for horseradish peroxidase-cosubstrate-h(2)o(2) systems was optimized. o-phenilendiamine, phenol, hydroquinone, pyrocatechol, p-chlorophenol, p-aminophenol and 3,3'-5,5'-tetramethylbenzidine were evaluated as cosubstrates of horseradish peroxidase (hrp) enzyme. therefore, the reaction time, the addition sequence of the substrates, the cosubstrate:h(2)o(2) ratio and the electrochemical techniques were elected by one-factor optimization assays while the buffer ph, the ...201222265528
protein functionalized titania particle as a nanocarrier in a multiple signal antibody amplification strategy for ultrasensitive chemiluminescent immunoassay.a simple and sensitive method for amplified chemiluminescent immunoassay has been developed by using multiple signal antibodies functionalized titania nanoparticle as tracer. this nanocomposite was fabricated by sequentially conjugating bovine serum albumin and horseradish peroxidase-labeled antibody onto the surface of titania nanoparticles using glutaraldehyde as the linkage. after a sandwich immuno-binding process, the captured nanocomposite tracer greatly catalyzed the luminol chemiluminesce ...201222265572
sortase a-catalyzed site-specific coimmobilization on microparticles via streptavidin.a microparticle surface was designed by the unique method incorporating streptavidin-biotin affinity and sortase a (srta)-catalyzed transpeptidation. leucine-proline-glutamate-threonine-glycine-tagged streptavidin (stav-lpetg)was immobilized on the surface using streptavidin-biotin affinity, and ggggg-tagged red fluorescent protein (gly5-rfp) was conjugated with srta. biotinylated fluorescein isothiocyanate (biotin-fitc) was then bound to residual biotin-binding sites in stav-lpetg. the resultin ...201222276782
obtention of plant peroxidase and its potential for the decolorization of the reactive dye remazol turquoise g 133%.peroxidases can be used in the decolorization process. there is a growing interest for new sources of this enzyme and for obtaining economically viable processes. in this work, a low-cost vegetable peroxidase extraction process is proposed; the resulting enzyme is characterized to determine its optimum ph, temperature, and stability conditions, and it is then applied in the decolorization of reactive dye remazol turquoise g 133%. the turnip peroxidase (tp) was utilized as an enzymatic source. th ...201222277225
electrochemical surface plasmon resonance measurement based on gold nanohole array fabricated by nanoimprinting technique.in this paper, we describe our development of an electrochemical surface plasmon resonance (ec-spr) measurement device based on a bottom-filled gold nanohole array. the polymer based gold nanohole array was fabricated with a uv nanoimprint technique and electron beam gold deposition. direct reflection mode measurement was used to monitor the spr dip in the reflection spectra. a cyclic voltammogram was also operated by using the standard three electrodes containing working electrode having a gold ...201222283116
structural characterization of horseradish peroxidase using exafs spectroscopy. evidence for fe = o ligation in compounds i and ii. 198622283292
measurement of rates and equilibria for keto-enol tautomerism of aldehydes using horseradish peroxidase compound i. 198622283318
a novel label-free fluorescent sensor for the detection of potassium ion based on dnazyme.a novel label-free and sensitive fluorescent aptasensor for the detection of potassium ion (k(+)) was developed based on the horseradish peroxidase-mimicking dnazyme (hrp-dnazyme). in this work, we selected a k(+)-stabilized single stranded dna (ssdna) with g-rich sequence as the recognition element. in the presence of k(+), the g-rich dna folded into the g-quadruplex structure, and then hemin can bind to the g-quadruplex structure as a co-factor and form hrp-dnazyme. 3-(p-hydroxyphenyl)-propano ...201222284459
highly efficient quenching of electrochemiluminescence from cds nanocrystal film based on biocatalytic deposition.aiming to find an alternative electrochemiluminescence (ecl) quenching route of high efficiency, biocatalytic precipitation (bcp) was firstly coupled with ecl for investigating its insulating effect on ecl. experimental data revealed that an insulating layer could be formed via bcp onto the electrode surface, inhibiting the reaction between the coreactant and luminophore and thereby impairing the ecl emission dramatically. since the extent of insulating effect associated strongly with analyte co ...201222284512
use of msg1 protein in a novel blocking elisa for the detection of mycoplasma suis infection.a blocking elisa based on the monoclonal antibody (mab) 1a7 was developed to detect antibodies against mycoplasma suis. the mab was produced by immunising balb/c mice with recombinant msg1 protein (rmsg1) from m. suis expressed in e. coli. following identification by western blotting, the mab was purified and labelled with horseradish peroxidase. the parameters of the elisa were optimised, and the cut-off value determined as 36.35% by analysing the percentage inhibition of m. suis negative serum ...201222285443
food as a source for quorum sensing inhibitors: iberin from horseradish revealed as a quorum sensing inhibitor of pseudomonas aeruginosa.foods with health-promoting effects beyond nutritional values have been gaining increasing research focus in recent years, although not much has been published on this subject in relation to bacterial infections. with respect to treatment, a novel antimicrobial strategy, which is expected to transcend problems with selective pressures for antibiotic resistance, is to interrupt bacterial communication, also known as quorum sensing (qs), by means of signal antagonists, the so-called qs inhibitors ...201222286987
application of optimized chemiluminescence assay for determination of the antioxidant capacity of herbal extracts.a chemiluminescence (cl) assay for the determination of antioxidant capacity (aoc) has been optimized and applied to analyses of herbal extracts in the present study. the optimal concentrations of reagents (luminol, h2o2, horseradish peroxidase) have been determined, as well as the optimal reaction conditions (wavelength, ph, temperature, sample volume). all of the measurements were performed at the emission maximum of the oxidized form of luminol (425 nm). the optimal concentrations of the reag ...201622290787
applications of nanomaterials in electrochemical enzyme biosensors.a biosensor is defined as a kind of analytical device incorporating a biological material, a biologically derived material or a biomimic intimately associated with or integrated within a physicochemical transducer or transducing microsystem. electrochemical biosensors incorporating enzymes with nanomaterials, which combine the recognition and catalytic properties of enzymes with the electronic properties of various nanomaterials, are new materials with synergistic properties originating from the ...200922291522
patterned biocatalytic films via one-step self-assembly.patterned porous films containing enzymes have been facilely prepared via a one-step breath figure process, which is based on the self-assembly of horseradish peroxidase nanoparticles that show good resistance to organic solvents. the patterned biohybrid films possess robust catalytic activity.201222293594
signal amplification by enzymatic reaction in an immunosensor based on localized surface plasmon resonance (lspr).an enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (lspr). the reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (ni) surfaces. for lspr sensing, the gold ni surface was fabricated on glass substrates using vacuum evaporation and heat treatment. the interferon-γ (ifn-γ) capture antibody was immobilized on the gold nis, followed by binding of ifn-γ to the antibod ...201022294913
enzyme-nanoparticle conjugates at oil-water interface for amplification of electrochemical immunosensing.in the current research, the preparation of biocatalytic capsule (ab(2)-catalytic capsule) was based on utilizing "oil" (organic compound) to entrap the nano-au/hrp/hafp bioconjugates, which were produced by horseradish peroxidase (hrp) and hrp labeled anti-afp (hafp) co-conjugated on gold nanoparticles (nano-au). for the first time, a signal amplification strategy was introduced by the prepared biocatalytic capsule as labels for electrochemical immunodetection of α-1-fetoprotein (afp). the tran ...201222296802
glucose oxidase/horseradish peroxidase co-immobilized at a cnt-modified graphite electrode: towards potentially implantable biocathodes. 201222298433
development of an electrochemical immunoassay for detection of gatifloxacin in swine urine.to detect gatifloxacin (gat) residue in swine urine, an electrochemical immunoassay was established. an indirect competitive immunoassay was developed, in which the coating antigen is immobilized in an enzyme-linked immunosorbent assay (elisa) plate and gat residue from the sample competes with the limited binding sites in added anti-gat antibody. horseradish peroxidase (hrp) conjugated to goat anti-rabbit igg was used as the enzymatic label. a carbon fiber working electrode was constructed and ...201222302425
synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model.quorum sensing (qs)-deficient pseudomonas aeruginosa biofilms formed in vitro are more susceptible to tobramycin than qs-proficient p. aeruginosa biofilms, and combination treatment with a qs inhibitor (qsi) and tobramycin shows synergistic effects on the killing of in vitro biofilms. we extended these results to an in vivo p. aeruginosa foreign-body biofilm model. the effect of treatment initiated prophylactically was compared with treatment initiated 11 days post-insertion.201222302561
polyester sulphonic acid interstitial nanocomposite platform for peroxide biosensor.a novel enzyme immobilization platform was prepared on a platinum disk working electrode by polymerizing aniline inside the interstitial pores of polyester sulphonic acid sodium salt (pesa). scanning electron microscopy study showed the formation of homogeneous sulphonated polyaniline (pani) nanotubes (∼90 nm) and thermogravimetric analysis (tga) confirmed that the nanotubes were stable up to 230 °c. the pani:pesa nanocomposite showed a quasi-reversible redox behaviour in phosphate buffer saline ...200922303157
chemical kinetics and interactions involved in horseradish peroxidase-mediated oxidative polymerization of phenolic compounds.the primary objective of this research was to evaluate various factors that affect the reaction rate of oxidative coupling (oxc) reaction of phenolic estrogens catalyzed by horseradish peroxidase (hrp). kinetic parameters were obtained for the conversion of phenol as well as natural and synthetic estrogens estrone (e(1)), 17β-estradiol (e(2)), estriol (e(3)), and 17α-ethinylestradiol (ee(2)). molecular orbital theory and autodock software were employed to analyze chemical properties and substrat ...201222305176
triplex signal amplification for electrochemical dna biosensing by coupling probe-gold nanoparticles-graphene modified electrode with enzyme functionalized carbon sphere as tracer.an ultrasensitive electrochemical dna biosensor was constructed by assembling probe labeled gold nanoparticles (ssdna-aunp) on electrochemically reduced graphene oxide (ergo) modified electrode with thiol group tagged (gt) dna strand (d(gt)(29)sh) and coupling with horseradish peroxidase (hrp) functionalized carbon sphere (cns) as tracer. the heteronanostructure formed on the biosensor surface appeared relatively good conductor for accelerating the electron transfer, while the hrp tagged cns pro ...201222305443
dna template-assisted modulation of horseradish peroxidase activity.the precise alignment of dna molecules by watson-crick base-pairing combined with its polymeric characteristics have allowed dna to be used as a template or scaffold for assembling materials. in this work, we investigate the role of calf-thymus dna as a template for enhancing the horseradish peroxidase (hrp)-mediated oxidation of phenol and phenolic derivatives. the hrp-catalyzed oxidation of phenol into polyphenolic products and in presence of 4-aminoantipyrine into quinoneimine dye complexes i ...201222305796
neuroprotective effects of testosterone on motoneuron and muscle morphology following spinal cord injury.treatment with testosterone is neuroprotective/neurotherapeutic after a variety of motoneuron injuries. here we assessed whether testosterone might have similar beneficial effects after spinal cord injury (sci). young adult female rats received either sham or t9 spinal cord contusion injuries and were implanted with blank or testosterone-filled silastic capsules. four weeks later, motoneurons innervating the vastus lateralis muscle of the quadriceps were labeled with cholera toxin-conjugated hor ...201222314886
chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening.a sensitive chemiluminescence (cl) imaging immunoassay method for detection of multiple tumor markers with high throughput, easy operation, and low cost was developed. the immunosensor array was prepared by covalently immobilizing capture antibodies on corresponding sensing sites on a silanized disposable glass chip. gold nanoparticle-based bioconjugates with a high molar ratio of horseradish peroxidase (hrp) to detection antibodies were used for signal amplification. under a sandwich immunoassa ...201222320247
highly sensitive chemiluminescent analysis of residual bovine serum albumin (bsa) based on a pair of specific monoclonal antibodies and peroxyoxalate-glyoxaline-phppa dimer chemiluminescent system in vaccines.enzyme-linked immunosorbent assay (elisa), horseradish peroxidase (hrp)-catalyzed fluorescent reaction, and oxalate chemiluminescence analysis have been combined to develop a highly sensitive, simple, and rapid method for analysis of bovine serum albumin (bsa) based on a pair of specific monoclonal antibodies in vaccines. a typical "sandwich type" immunoassay was used. reaction of 3-(4-hydroxyphenyl propionate) (phppa) with hydrogen peroxide-urea, catalyzed by hrp, produced fluorescence of 3-(4- ...201222328250
development of a solid-phase extraction coupling chemiluminescent enzyme immunoassay for determination of organophosphorus pesticides in environmental water samples.solid-phase extraction (spe) and direct competitive chemiluminescence enzyme immunoassay (dccl-eia) were combined for the detection of organophosphorus pesticides (ops) in environmental water samples. dccl-eia based on horseradish peroxidase labeled with a broad-specificity monoclonal antibody against ops was developed, and the effects of several physicochemical parameters on dccl-eia performance were studied. spe was used for the pretreatment of water samples to remove interfering substances an ...201222329773
recombinant protein expression in pichia pastoris strains with an engineered methanol utilization pathway.βackground: the methylotrophic yeast pichia pastoris has become an important host organism for recombinant protein production and is able to use methanol as a sole carbon source. the methanol utilization pathway describes all the catalytic reactions, which happen during methanol metabolism. despite the importance of certain key enzymes in this pathway, so far very little is known about possible effects of overexpressing either of these key enzymes on the overall energetic behavior, the productiv ...201222330134
displacement immunoassay for the detection of ochratoxin a using ochratoxin b modified glass beads.we report here the development of a new assay for the detection of ochratoxin a (ota) based on the use of its dechlorinated analogue, ochratoxin b (otb), in a displacement immunoassay. otb was immobilised on controlled-pore glass beads followed by the binding of anti-ota antibody, with anti-igg antibody peroxidase conjugate used as a label. in this manner, an original bio-sensing material was obtained. upon incubation of this material with ota, the toxin competes with otb for the binding sites o ...201222331050
synthesis of a unique high-performance poly-horseradish peroxidase complex to enhance sensitivity of immunodetection systems.because early detection is the first step in successful therapy, increasing the sensitivity of detection systems has always been considered as one of the major trends in development of these technologies. therefore, we have fabricated a high-performance poly-horseradish peroxidase (hrp) complex and analyzed it in different formats of immunodetection systems. to construct this complex, dextran-aldehyde was prepared by oxidation of dextran in the presence of sodium periodate. activated polymer was ...201622332744
luminescence based enzyme-labeled phage (phazyme) assays for rapid detection of shiga toxin producing escherichia coli serogroups.most diagnostic approaches for shiga toxin producing escherichia coli (stec) have been designed to detect only serogroup o157 that causes a majority, but not all stec related outbreaks in the united states. therefore, there is a need to develop methodology that would enable the detection of other stec serogroups that cause disease. three bacteriophages (phages) that infect stec serogroups o26, o103, o111, o145 and o157 were chemically labeled with horseradish peroxidase (hrp). the enzyme-labeled ...201122334866
enzyme-regulated activation of dnazyme: a novel strategy for a label-free colorimetric dna ligase assay and ligase-based biosensing.the dna nick repair catalyzed by dna ligase is significant for fundamental life processes, such as the replication, repair, and recombination of nucleic acids. here, we have employed ligase to regulate dnazyme activity and developed a homogeneous, colorimetric, label-free and dnazyme-based strategy to detect dna ligase activity. this novel strategy relies on the ligation-trigged activation or production of horseradish peroxidase mimicking dnazyme that catalyzes the generation of a color change s ...201222337073
magnetic bead-sensing-platform-based chemiluminescence resonance energy transfer and its immunoassay application.a competitive immunoassay based on chemiluminescence resonance energy transfer (cret) on the magnetic beads (mbs) is developed for the detection of human immunoglobulin g (igg). in this protocol, carboxyl-modified mbs were conjugated with horseradish peroxidase (hrp)-labeled goat antihuman igg (hrp-anti-igg) and incubated with a limited amount of fluorescein isothiocyanate (fitc)-labeled human igg to immobilize the antibody-antigen immune complex on the surface of the mbs, which was further incu ...201222339489
horseradish peroxidase: modulation of properties by chemical modification of protein and heme.horseradish peroxidase (hrp) is one of the most studied enzymes of the plant peroxidase superfamily. hrp is also widely used in different bioanalytical applications and diagnostic kits. the methods of genetic engineering and protein design are now widely used to study the catalytic mechanism and to improve properties of the enzyme. here we review the results of another approach to hrp modification-through the chemical modification of amino acids or prosthetic group of the enzyme. computer models ...201122339595
nano-structured arrays for multiplex analyses and lab-on-a-chip applications.nanospheres lithographic (nsl) method has been used to fabricate nano-structured arrays (nas) of hexagonally close-packed gold (au) using polystyrene beads [ps, diameter ∼300 nm] as mask. the developed na was incorporated with a customized and cheap microfluidics system to demonstrate its applicability as an alternative easy and efficient platform for multiplex analysis and lab-on-a-chip applications. the chip functionality was demonstrated with horseradish peroxidase (hrp) and anti-hrp antibody ...201222342717
selective (1)o(2) quenchers, oligostilbenes, from vitis wilsonae: structural identification and biogenetic relationship.two previously unknown resveratrol trimers named wilsonols a-b, as well as a resveratrol tetramer named wilsonol c, were isolated from vitis wilsonae veitch, together with 12 known oligostilbenes. their chemical structures have been elucidated by detailed analyses of 1d and 2d nmr spectroscopic data, as well as chemical evidence obtained via either catalysis with hrp (horseradish peroxidase) and h(2)o(2) (hydrogen peroxide), acid, or uv irradiation. during the chemical processes, a biomimetic re ...201222342782
enzymatically cross-linked gelatin-phenol hydrogels with a broader stiffness range for osteogenic differentiation of human mesenchymal stem cells.an injectable hydrogel system, composed of gelatin-hydroxyphenylpropionic acid (gtn-hpa) conjugates chemically cross-linked by an enzyme-mediated oxidation reaction, has been designed as a biodegradable scaffold for tissue engineering. in light of the role of substrate stiffness on cell differentiation, we herein report a newly improved gtn hydrogel system with a broader range of stiffness control that uses gtn-hpa-tyramine (gtn-hpa-tyr) conjugates to stimulate the osteogenic differentiation of ...201222343003
biofunctionalized dendritic polyaniline nanofibers for sensitive electrochemical immunoassay of biomarkers.multi-armed dendritic polyaniline nanofibers (mpanfs) were first synthesized and functionalized with horseradish peroxidase (hrp) and carcinoembryonic antibody (anti-cea) for highly efficient electrochemical immunoassay of carcinoembryonic antigen (cea, as a model analyte here) in this work. transmission electron microscope (tem) and scanning electron microscope (sem) techniques were employed to characterize the synthesized mpanfs. by using anti-cea-conjugated core-shell gold-fe(3)o(4) nanocompo ...201222355804
magnetosomes eliminate intracellular reactive oxygen species in magnetospirillum gryphiswaldense msr-1.magnetotactic bacteria synthesize magnetic particles called magnetosomes that cause them to orient to their external magnetic fields. however, the physiological significance and other possible functions of these magnetosomes have not been explored in detail. in this study, we have investigated the biological functions of magnetosomes with respect to their ability to scavenge reactive oxygen species (ros) in magnetospirillum gryphiswaldense msr-1. to assess the changes in ros levels under differe ...201222360568
a hydrophilic and hydrophobic organic solvent mixture enhances enzyme stability in organic media.binary mixtures of hydrophilic and hydrophobic solvents were assessed for their ability to balance enzyme activity with the conservation of enzyme stability in organic media. acetone, dioxane and dodecane were chosen as model organic solvents, and subtilisin carlsberg and horseradish peroxidase (hrp) were chosen as model enzymes. residual enzyme activities were measured to monitor enzyme stability, and the fluorescence intensity of hrp was monitored to investigate structural changes due to the p ...201222361965
a bispecific antibody based assay shows potential for detecting tuberculosis in resource constrained laboratory settings.the re-emergence of tuberculosis (tb) as a global public health threat highlights the necessity of rapid, simple and inexpensive point-of-care detection of the disease. early diagnosis of tb is vital not only for preventing the spread of the disease but also for timely initiation of treatment. the later in turn will reduce the possible emergence of multi-drug resistant strains of mycobacterium tuberculosis. lipoarabinomannan (lam) is an important non-protein antigen of the bacterial cell wall, w ...201222363820
electrochemical immunosensor for carcinoembryonic antigen based on nanosilver-coated magnetic beads and gold-graphene nanolabels.a novel redox-active magnetic nanostructure was synthesized by using a wet chemical method for high-efficiency electrochemical immunoassay of carcinoembryonic antigen (cea, as a model analyte). the nanostructures based on the combination of a magnetic nanocore, a layer of electroactive poly(o-phenylenediamine) (ppd), and a silver metallic shell displayed good adsorption properties for the attachment of anti-cea antibody selective to cea. the magnetic nanostructure presented good redox behaviors ...201222365686
hemigossypol, a constituent in developing glanded cottonseed (gossypium hirsutum).gossypol is a dimeric sesquiterpenoid first identified in cottonseed, but found in various tissues in the cotton plant including the seed. from its first discovery, it was assumed that hemigossypol was the biosynthetic precursor of gossypol. previous studies established that peroxidase (either from horseradish or from cottonseed) converts hemigossypol to gossypol. however, hemigossypol has never been identified in healthy cottonseed. in a temporal study using hplc and lc-ms, hemigossypol was ide ...201222369216
assessment of reactive oxygen species production in cultured equine skeletal myoblasts in response to conditions of anoxia followed by reoxygenation with or without exposure to peroxidases.to culture equine myoblasts from muscle microbiopsy specimens, examine myoblast production of reactive oxygen species (ros) in conditions of anoxia followed by reoxygenation, and assess the effects of horseradish peroxidase (hrp) and myeloperoxidase (mpo) on ros production.201222369537
identification of nitrite-reducing bacteria using sequential mrna fluorescence in situ hybridization and fluorescence-assisted cell sorting.sequential mrna fluorescence in situ hybridization (mrna fish) and fluorescence-assisted cell sorting (smrff) was used for the identification of nitrite-reducing bacteria in mixed microbial communities. an oligonucleotide probe labeled with horseradish peroxidase (hrp) was used to target mrna of nirs, the gene that encodes nitrite reductase, the enzyme responsible for the dissimilatory reduction of nitrite to nitric oxide. clones for nirs expression were constructed and used to provide proof of ...201222370876
gold nanoparticles/horseradish peroxidase encapsulated polyelectrolyte nanocapsule for signal amplification in listeria monocytogenes detection.bioconjugate nanocapsules were fabricated by using polystyrene sulfonate (pss) to encapsulate gold nanoparticles (aunps) bearing adsorbed horseradish peroxidase (hrp). the average size of nanocapsule was in a range 150-400 nm. the efficiency of the capsules to enhance signals in an immunoassay was demonstrated by using an enzyme linked immunosorbent assay (elisa) to detect the food-borne pathogen -listeria monocytogenes. the antibody adsorbed onto the pss shell of the nanocapsules provided the r ...201222386483
electrochemical detection of natural dna damage induced by in situ peroxidase-generated reactive nitrogen species in dna layer-by-layer films.the no(2)• radical is one kind of reactive nitrogen species and can be generated by horseradish peroxidase (hrp)-catalyzed oxidation of nitrite (no(2)(-)) in the presence of hydrogen peroxide (h(2)o(2)) and may cause dna damage in living system. in the present work, {pdda/dna}(4) layer-by-layer (lbl) films assembled with poly(diallyldimethylammonium chloride) (pdda) and natural double-stranded dna on electrodes were used to detect dna damage induced by no(2)• radicals generated from the hrp+h(2) ...201222387104
target-dependent axonal sprouting following vagal-hypoglossal nerve anastomosis in cats.to investigate the relationships between the axonal sprouting and target neurotization by central neurons after nerve heteroconnection.200022387739
in praise of h₂o₂, the versatile ros, and its vanadium complexes.hydrogen peroxide (h₂o₂) is generated in mitochondria in aerobic cells as a minor product of electron transport, is inhibited selectively by phenolic acids (in animals) or salicylhydroxamate (in plants) and is regulated by hormones and environmental conditions. failure to detect this activity is due to presence of h₂o₂-consuming reactions or inhibitors present in the reaction mixture. h₂o₂ has a role in metabolic regulation and signal transduction reactions. a number of enzymes and cellular acti ...201222394337
effects of operating parameters on the efficiency of liposomal encapsulation of enzymes.encapsulation of active proteins in the hydrophilic core of vesicular liposomes is important for developing a therapeutic protein carrier system. the efficiency of liposomal encapsulation of proteins is generally low. a better understanding of the fundamental mechanisms of encapsulation is needed to increase this efficiency. in this study we investigated the effects of operating parameters such as phospholipid concentration, buffer ph and ionic strength, protein size and surface charge, and lipo ...201222398367
direct electrochemistry of horseradish peroxidase-gold nanoparticles conjugate.we have studied the direct electrochemistry of horseradish peroxidase (hrp) coupled to gold nanoparticles (aunp) using electrochemical techniques, which provide some insight in the application of biosensors as tools for diagnostics because hrp is widely used in clinical diagnostics kits. aunp capped with (i) glutathione and (ii) lipoic acid was covalently linked to hrp. the immobilized hrp/aunp conjugate showed characteristic redox peaks at a gold electrode. it displayed good electrocatalytic re ...200922399945
core/shell nanofibers with embedded liposomes as a drug delivery system.the broader application of liposomes in regenerative medicine is hampered by their short half-life and inefficient retention at the site of application. these disadvantages could be significantly reduced by their combination with nanofibers. we produced 2 different nanofiber-liposome systems in the present study, that is, liposomes blended within nanofibers and core/shell nanofibers with embedded liposomes. herein, we demonstrate that blend electrospinning does not conserve intact liposomes. in ...201222401557
quantification of domoic acid in shellfish samples by capillary electrophoresis-based enzyme immunoassay with electrochemical detection.a new method was developed to quantify domoic acid by capillary electrophoresis-based enzyme immunoassay (ce-eia) with electrochemical (ec) detection. the method was based on noncompetitive immunoreaction between free domoic acid antigen (ag) and excessive amount of horseradish peroxidase (hrp)-labeled antidomoic acid antibody tracer (ab*) in liquid phase. then the bound enzyme-labeled complex (ab*-ag) and unbound ab* were separated by ce, and the system of hrp catalyzing h(2)o(2)/o-aminophenol ...201222406515
interaction between la(iii) and proteins on the plasma membrane of horseradish.lanthanum (la) is an important rare earth element in the ecological environment of plant. the proteins on the plasma membrane control the transport of molecules into and out of cell. it is very important to investigate the effect of la(iii) on the proteins on the plasma membrane in the plant cell. in the present work, the interaction between la(iii) and proteins on the plasma membrane of horseradish was investigated using optimization of the fluorescence microscopy and fluorescence spectroscopy. ...201222407212
immobilization of hrp in mesoporous silica and its application for the construction of polyaniline modified hydrogen peroxide biosensor.polyaniline (pani), an attractive conductive polymer, has been successfully applied in fabricating various types of enzyme-based biosensors. in this study, we have employed mesoporous silica sba-15 to stably entrap horseradish peroxidase (hrp), and then deposited the loaded sba-15 on the pani modified platinum electrode to construct a ga/sba-15(hrp)/pani/pt biosensor. the mesoporous structures and morphologies of sba-15 with or without hrp were characterized. enzymatic protein assays were employ ...200922408546
facile fabrication of gold nanoparticles-poly(vinyl alcohol) electrospun water-stable nanofibrous mats: efficient substrate materials for biosensors.electrospun nanofibrous mats are intensively studied as efficient scaffold materials applied in the fields of tissue engineering, catalysis, and biosensors due to their flexibility and porosity. in this paper, we report a facile route to fabricate gold nanoparticles-poly(vinyl alcohol) (au nps-pva) hybrid water stable nanofibrous mats with tunable densities of au nps and further demonstrate the potential application of as-prepared au nps-pva nanofibrous mats as efficient biosensor substrate mate ...201222409429
an electrochemically reduced graphene oxide-based electrochemical immunosensing platform for ultrasensitive antigen detection.we present an electrochemically reduced graphene oxide (ergo)-based electrochemical immunosensing platform for the ultrasensitive detection of an antigen by the sandwich enzyme-linked immunosorbent assay (elisa) protocol. graphene oxide (go) sheets were initially deposited on the amine-terminated benzenediazonium-modified indiun tin oxide (ito) surfaces through both electrostatic and π-π interactions between the modified surfaces and go. this deposition was followed by the electrochemical reduct ...201222409529
diagnostic potential of fasciola gigantica-derived 14.5 kda fatty acid binding protein in the immunodiagnosis of bubaline fascioliasis.the 14.5 kda fatty acid binding protein (fabp) was isolated from the crude extract of adult fasciola gigantica worms. polyclonal anti-fabp igg was generated in rabbits immunized with prepared fabp antigen. sandwich enzyme-linked immunosorbent assay (elisa) was applied to detect coproantigen in stools and circulating fasciola antigen (ca) in sera of 126 water buffaloes by using purified and horseradish peroxidase (hrp)-conjugated anti-fabp igg. sandwich elisa sensitivity was 96.97% and 94.95%; wh ...201322409954
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