Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| vertical and horizontal transmission of tilapia larvae encephalitis virus: the bad and the ugly. | impairment of innate immunity in tilapia larvae after vertical and horizontal infection with the newly characterized tilapia larvae encephalitis virus (tlev) was accessed by evaluation of cell-mediated reactive oxygen species (ros) production in affected fish with the use of horseradish peroxidase-amplified luminol-dependent chemiluminescence assay. the priming in-vivo infection with tlev resulted in downregulation of ros response in both vertically- and horizontally-infected fish; this suppress ... | 2011 | 21131016 |
| the expression of nadph oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line a549. | controlled production of reactive oxygen species (ros) by nadph oxidases in non-phagocytic cells has recently been suggested to participate in the regulation of cellular functions. due to the role of ros in control of cellular functions, precise and accurate detection of ros is of essential importance. however, various methodological approaches currently used for ros determination vary in sensitivity, specificity, as well as in requirements for specialized equipment. in this study, human lung ep ... | 2010 | 21138653 |
| site-specific protein cross-linking by peroxidase-catalyzed activation of a tyrosine-containing peptide tag. | protein modification methods represent fundamental techniques that are applicable in many fields. in this study, a site-specific protein cross-linking based on the oxidative tyrosine coupling reaction was demonstrated. in the presence of horseradish peroxidase (hrp) and h(2)o(2), tyrosine residues undergo one-electron oxidation reactions and form radicals in their phenolic moieties, and these species subsequently react with each other to form dimers or further react to generate polymers. here, a ... | 2010 | 21142129 |
| development of porous alginate-based scaffolds covalently cross-linked through a peroxidase-catalyzed reaction. | porous scaffolds are important in tissue engineering. we developed porous scaffolds from the hydrogels of an alginate derivative bearing phenolic hydroxyl groups. the hydrogels were prepared using horseradish peroxidase (hrp) to catalyze the cross-linking between the phenolic hydroxyl groups. a porous structure with a pore size of approx. 200 μm was developed through simultaneous water-extraction and ionic cross-linking by calcium ions by soaking frozen hydrogels in the mixture of ethanol and ca ... | 2011 | 21144141 |
| miniaturised hybrid immunoassay for high sensitivity analysis of aflatoxin m1 in milk. | an ultra-sensitive sandwich elisa was developed for detection of afm1 in milk. the assay involved the immobilization of rat monoclonal antibody of afm1 in 384 microtiter plate to capture afm1 antigen. this was detected by tracer secondary rabbit poly-clonal antibody labelled with horseradish peroxidase upon addition of a luminol-based substrate. milk samples with different fat percentage were analyzed after pre-treatment. linear range of afm1 detection 250-6.25 pg/ml was achieved in 3% fat milk. ... | 2010 | 21145226 |
| lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle. | lactobacilli have been barely studied in cows. we proposed isolate and characterize lactic acid bacteria from dairy cows as compared to those raised for meat production and elucidate the presence of strains with evident probiotic employment's potential. for this, isolation and quantification of lab mainly lactobacilli were realized from vaginal cattle samples in mrs medium. each selected microorganism was then briefly characterized. the math method was employed using hexadecane, xilene an toluen ... | 2010 | 21145403 |
| citrinin (cit) determination in rice samples using a micro fluidic electrochemical immunosensor. | the development of an electrochemical immunosensor incorporated in a micro fluidic cell for quantification of citrinin (cit) mycotoxin in rice samples is described for the first time. both cit present in rice samples and immobilized on a gold surface electrodeposited on a glassy carbon (gc) electrode modified with a cysteamine self-assembled monolayer were allowed to compete for the monoclonal mouse anti-cit igg antibody (mab-cit) present in solution. then, an excess of rabbit anti mouse igg (h+ ... | 2010 | 21147345 |
| overexpression of androgen receptors in target musculature confers androgen sensitivity to motoneuron dendrites. | the dendritic arbors of spinal motoneurons are dynamically regulated by a variety of factors, and several lines of evidence indicate that trophic interactions with the target musculature are of central importance. in highly androgen-sensitive motoneuron populations, androgens are thought to regulate motoneuron dendrites through their action at the receptor-enriched target musculature. using rats transgenically modified to overexpress androgen receptor (ar) in skeletal muscle, we directly tested ... | 2010 | 21147875 |
| a novel h2o2 sensor based on the enzymatically induced deposition of polyaniline at a horseradish peroxide/aligned single-wall carbon nanotubes modified au electrode. | a novel h(2)o(2) sensor based on enzymatically induced deposition of electroactive polyaniline (pani) at a horseradish peroxide (hrp)/aligned single-wall carbon nanotubes (swcnts) modified au electrode is fabricated, and its electrochemical behaviors are investigated. electrochemical impedance spectroscopy of the sensor confirmed the formation of pani on swcnts through the hrp catalytic reaction. cyclic voltammograms of pani/hrp/swcnts modified au electrodes showed a pair of well-defined redox p ... | 2011 | 21152626 |
| signal-enhancing thermosensitive liposomes for highly sensitive immunosensor development. | liposomes are potential candidates as nanovesicles for the development of detection systems with improved sensitivity and detection limits, due to their capacity to encapsulate diverse types of signal enhancing molecules. an amperometric immunosensor exploiting enzyme encapsulating thermosensitive liposomes for the ultrasensitive detection of carcinoembryonic antigen (cea) is reported. five different bioconjugation methods to link an anti-cea antibody to horseradish peroxidase (hrp) encapsulatin ... | 2011 | 21155541 |
| inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from dimorphandra mollis (fabaceae, caesalpinioideae). | astilbin (5,7,3',4'-tetrahydroxy-2,3-dihydroflavonol-3-ß-o-rhamnoside), a flavonoid with a large range of biological activities, was isolated from dimorphandra mollis, a shrub common to the brazilian cerrado. the purpose of this study is to verify the effects of astilbin on myeloperoxidase (mpo) and horseradish peroxidase (hrp), and its antioxidant activity against hypochlorous acid (hocl) and total antioxidant activity (tac) by the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical ... | 2010 | 21157633 |
| high sensitivity dna detection using gold nanoparticle functionalised polyaniline nanofibres. | polyaniline (pani) nanofibres (pani-nf) have been modified with chemically grown gold nanoparticles to give a nanocomposite material (pani-nf-aunp) and deposited on gold electrodes. single stranded capture dna was then bound to the gold nanoparticles and the underlying gold electrode and allowed to hybridise with a complementary target strand that is uniquely associated with the pathogen, staphylococcus aureus (s. aureus), that causes mastitis. significantly, cyclic voltammetry demonstrates that ... | 2010 | 21159503 |
| ultrastructural analysis of glutamate-immunopositive synapses onto the rat jaw-closing motoneurons during postnatal development. | the excitatory synapses on the jaw-closing (jc) motoneurons mediate the neuronal input that ensures smooth and rhythmic movements of the jaw. recently, we have shown that the neurotransmitter phenotype of the inhibitory boutons onto jc motoneurons shifts from gaba to glycine, and new inhibitory synapses onto jc motoneurons are continuously formed during postnatal development (paik et al. [2007] j. comp. neurol. 503:779–789). to test whether the developmental pattern of the excitatory synapses on ... | 2010 | 21162123 |
| [the lesion of csf contacting neurons in rat brain parenchyma inhibits the development of morphine dependence and withdrawal]. | to investigate the effect of csf contacting neurons (csf-cns) lesion in rat dorsal raphe nucleus (drn) on the scores of morphine withdrawal symptoms precipitated by naloxone and the nnos expression in dorsal horn of spinal cord, and study the relationship between the distal csf-cns in rat brain parenchyma and the development of morphine dependence and withdrawal. | 2007 | 21162265 |
| enzymatic degradation of a2e, a retinal pigment epithelial lipofuscin bisretinoid. | some forms of blinding macular disease are associated with excessive accumulation of bisretinoid lipofuscin in retinal pigment epithelial (rpe) cells of the eye. this material is refractory to lysosomal enzyme degradation. in addition to gene and drug-based therapies, treatments that reverse the accumulation of bisretinoid would be beneficial. thus, we have examined the feasibility of degrading the bisretinoids by delivery of exogenous enzyme. as proof of principle we report that horseradish per ... | 2011 | 21166406 |
| immunoperoxidase detection of neuronal antigens in full-thickness whole mount preparations of hollow organs and thick sections of central nervous tissue. | immunofluorescently stained whole mounts have proved useful for defining the innervation of the gut and large blood vessels. nerves supplying other hollow organs are usually studied in sections, which provide much less information. aiming to describe the entire innervation of rat uterus, we developed a method for immunoperoxidase staining of full-thickness whole mounts that allowed us to visualize all immunoreactive axons. uterine horns were dissected out, slit open, stretched, pinned flat and f ... | 2010 | 21167203 |
| antibody-targeted horseradish peroxidase associated with indole-3-acetic acid induces apoptosis in vitro in hematological malignancies. | indole-3-acetic acid (iaa), when oxidized by horseradish peroxidase (hrp), is transformed into cytotoxic molecules capable of inducing cell injury. the aim of this study was to test if, by targeting hematopoietic tumors with hrp-conjugated antibodies in association with iaa treatment, there is induction of apoptosis. we used two lineages of hematologic tumors: nb4, derived from acute promyelocytic leukemia (apl) and granta-519 from mantle cell lymphoma (mcl). we also tested cells from 12 patient ... | 2010 | 21168913 |
| electrochemical detection of extracellular hydrogen peroxide released from raw 264.7 murine macrophage cells based on horseradish peroxidase-hydroxyapatite nanohybrids. | a new method developed for the reliable determination of extracellular and intracellular h(2)o(2) is very useful for gaining a full understanding of the role that h(2)o(2) plays in pathology and physiology, and the relationship between h(2)o(2) and environmental stresses and lipid peroxidation. this work developed and validated an electrochemical approach for the determination of extracellular h(2)o(2) released from raw 264.7 murine macrophage cells. this approach is based on the electrocatalyti ... | 2011 | 21170446 |
| preparation of catalytically active, covalent α-polylysine-enzyme conjugates via uv/vis-quantifiable bis-aryl hydrazone bond formation. | covalent uv/vis-quantifiable bis-aryl hydrazone bond formation was investigated for the preparation of conjugates between α-poly-d-lysine (pdl) and either α-chymotrypsin (α-ct) or horseradish peroxidase (hrp). pdl and the enzymes were first modified via free amino groups with the linking reagents succinimidyl 6-hydrazinonicotinate acetone hydrazone (s-hynic, at ph 7.6) and succinimidyl 4-formylbenzoate (s-4fb, at ph 7.2), respectively. the modified pdl and enzymes were then conjugated at ph 4.7, ... | 2010 | 21171644 |
| dual amplification strategy for the fabrication of highly sensitive interleukin-6 amperometric immunosensor based on poly-dopamine. | an electrochemical immunosensor was studied for sensitive detection of interleukin-6 (il-6) based on a dual amplification mechanism resulting from au nanoparticles (aunp)-poly-dopamine (pdop) as the sensor platform and multienzyme-antibody functionalized aunp-pdop@carbon nanotubes (cnt). the stable and robust film, pdop, was used to immobilize biomolecules not only for the construction of the sensor platform, but also for the signal labeling. sensitivity was greatly amplified by using the specia ... | 2010 | 21174423 |
| design of a sandwich-mode amperometric biosensor for detection of pml/rarα fusion gene using locked nucleic acids on gold electrode. | in this study, a novel dna electrochemical probe (locked nucleic acid, lna) was designed and involved in constructing an electrochemical dna biosensor for detection of promyelocytic leukemia/retinoic acid receptor alpha (pml/rarα) fusion gene in acute promyelocytic leukemia for the first time. this biosensor was based on a 'sandwich' sensing mode, which involved a pair of lna probes (capture probe immobilized at electrode surface and biotinyl reporter probe as an affinity tag for streptavidin-ho ... | 2011 | 21177095 |
| a novel path for rapid transverse communication of vestibular signals in turtle cerebellum. | voltage-sensitive dye activity within the thin, unfoliated turtle cerebellar cortex (cb) was recorded in vitro during eighth cranial nerve (nviii) stimulation. short latency responses were localized to the middle of the lateral edges of both ipsilateral and contralateral cb [vestibulocerebellum (vcb)]. even with a severed contralateral cb peduncle, stimulation of the nviii ipsilateral to the intact peduncle evoked contralateral vcb responses with a mean latency of only 0.25 ms after the ipsilate ... | 2011 | 21178000 |
| botanical micelle and its application for direct electrochemical biosensor. | this paper is concerned about the entrapment of horseradish peroxidase (hrp) within botanical inositol hexakisphosphoric (ip(6)) micelles for the preparation of enzyme biosensor. the good affinity of ip(6) micelles with the enzyme provides naturally biocompatible microenvironment for the enzyme immobilization, achieving the direct electron transfer between hrp and electrode surface. the resulting biosensor to h(2)o(2) detection exhibits a low detection limit of 0.1 μmol l(-1) (s/n = 3), a quick ... | 2011 | 21183331 |
| facile synthesis of fe(3)o(4)@al(2)o(3) core-shell nanoparticles and their application to the highly specific capture of heme proteins for direct electrochemistry. | in this study, magnetic core-shell fe(3)o(4)@al(2)o(3) nanoparticles (nps) attached to the surface of a magnetic glassy carbon electrode (mgce) were used as a functional interface to immobilize several heme proteins including hemoglobin (hb), myoglobin (mb) and horseradish peroxidase (hrp) for fabricating protein/fe(3)o(4)@al(2)o(3) film. transmission electron microscope, uv-vis spectroscopy, electrochemical impedance spectroscopy, and cyclic voltammetry were used to characterize the films. with ... | 2011 | 21185712 |
| ultrasensitive electrochemical immunoassay for brca1 using bmim·bf₄-coated sba-15 as labels and functionalized graphene as enhancer. | brcal is an anti-oncogene in women, who are genetically predisposed to breast and ovary cancer. the detection of brca1 can offer an opportunity to characterize the function of genetic features in breast and ovarian cancer and to screen breast or ovarian cancer patients. in this study, we designed a new label and fabricated a novel sandwich-type electrochemical immunoassay for the ultrasensitive detection of brcal. horseradish peroxidase (hrp) was entrapped in the pores of amino-group functionali ... | 2010 | 21186053 |
| investigating the structural and functional effects of mutating asn glycosylation sites of horseradish peroxidase to asp. | horseradish peroxidase (hrp) has long attracted intense research interest and is used in many biotechnological fields, including diagnostics, biosensors, and biocatalysis. enhancement of hrp catalytic activity and/or stability would further increase its applications. one of the problems with heterologus expression of hrp especially in prokaryotic host is lack of glycosylation that affects it's stability toward h(2)o(2) and thermal inactivation. in this study, two asparagine residues which consti ... | 2010 | 21193964 |
| sensitive electrochemical immunosensor for cancer biomarker with signal enhancement based on nitrodopamine-functionalized iron oxide nanoparticles. | a novel electrochemical immunosensor for sensitive detection of cancer biomarker prostate specific antigen (psa) based on nitrodopamine (nda) functionalized iron oxide nanoparticles (nda-fe(3)o(4)) is described. nda-fe(3)o(4) was used both for the immobilization of primary anti-psa antibody (ab(1)) and as secondary anti-psa antibody (ab(2)) label. for the preparation of the label, mediator thionine (th) was first conjugated onto nda-fe(3)o(4) based on the amino groups of nda, and then the amino ... | 2011 | 21195601 |
| functionalized graphene oxide as a nanocarrier in a multienzyme labeling amplification strategy for ultrasensitive electrochemical immunoassay of phosphorylated p53 (s392). | p53 phosphorylation plays an important role in many biological processes and might be used as a potential biomarker in clinical diagnoses. we report a new electrochemical immunosensor for ultrasensitive detection of phosphorylated p53 at ser392 (phospho-p53(392)) based on graphene oxide (go) as a nanocarrier in a multienzyme amplification strategy. greatly enhanced sensitivity was achieved by using the bioconjugates featuring horseradish peroxidase (hrp) and p53(392) signal antibody (p53(392)ab( ... | 2011 | 21210663 |
| a unique coxiella burnetii lipoprotein involved in metal binding (limb). | coxiella burnetii is the bacterial agent of q fever in humans. here, we describe a unique, ∼7.2 kda, surface-exposed lipoprotein involved in metal binding which we have termed limb. limb was initially identified as a potential metal-binding protein on far-western (fw) blots containing whole-cell lysate proteins when probed with nickel-coated horseradish peroxidase (ni-hrp) and developed with a chemiluminescent hrp substrate. the corresponding identity of limb as cbu1224a was established b ... | 2011 | 21212117 |
| inkjet-printed gold nanoparticle electrochemical arrays on plastic. application to immunodetection of a cancer biomarker protein. | electrochemical detection combined with nanostructured sensor surfaces offers potentially low-cost, high-throughput solutions for detection of clinically significant proteins. inkjet printing offers an inexpensive non-contact fabrication method for microelectronics that is easily adapted for incorporating into protein immunosensor devices. herein we report the first direct fabrication of inkjet-printed gold nanoparticle arrays, and apply them to electrochemical detection of the cancer biomarker ... | 2011 | 21212889 |
| the enzyme-mediated activation of radical source reaction: a new approach to identify partners of a given molecule in membrane microdomains. | important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. however, it is difficult to assess the issue of how they assemble under physiological conditions. we developed a new approach to identify partners of a given molecule on the cell surface in living cells. the important feature of this system, termed as enzyme-mediated activation of radical source, is that activation of c ... | 2011 | 21214558 |
| horseradish peroxidase dye tracing and embryonic statoacoustic ganglion cell transplantation in the rat auditory nerve trunk. | at present severe damage to hair cells and sensory neurons in the inner ear results in non-treatable auditory disorders. cell implantation is a potential treatment for various neurological disorders and has already been used in clinical practice. in the inner ear, delivery of therapeutic substances including neurotrophic factors and stem cells provide strategies that in the future may ameliorate or restore hearing impairment. in order to describe a surgical auditory nerve trunk approach, in the ... | 2011 | 21215730 |
| activity of the c-terminal-dependent vacuolar sorting signal of horseradish peroxidase c1a is enhanced by its secondary structure. | plant class iii peroxidase (prx) catalyzes the oxidation and oxidative polymerization of a variety of phenolic compounds while reducing hydrogen peroxide. prx proteins are classified into apoplast type and vacuole type based on the absence or the presence of c-terminal propeptides, which probably function as vacuolar sorting signals (vsss). in this study, in order to improve our understanding of vacuole-type prx, we analyzed regulatory mechanisms of vacuolar sorting of a model vacuole-type prx, ... | 2011 | 21216746 |
| fluorescent derivatization of xanthurenic acid and nicotinic acid with horseradish peroxidase in the presence of excess hydrogen peroxide. | the fluorescent derivatization of tryptophan metabolites (xanthurenic acid, nicotinic acid, picolinic acid, and 3-hydroxyanthranilic acid) by the catalytic activity of horseradish peroxidase (hrp) was investigated in the presence of excess h(2)o(2). non-fluorescent xanthurenic acid (xa) and nicotinic acid (na) were converted into a fluorescent compound with maximum excitation and emission wavelengths at 325 and 425 nm, and 318 and 380 nm, respectively. this fluorescent derivatization was develop ... | 2011 | 21233570 |
| in situ molecular detection of ischemic cells by enhanced protein direct electron transfer on a unique horseradish peroxidase-au nanoparticles-polyaniline nanowires biofilm. | to overcome shortcomings of the ex situ approaches, in situ detection using h(2)o(2) molecules to diagnose ischemia through enhanced protein direct electron transfer on a unique horseradish peroxidase-au nanoparticles-polyaniline nanowires biofilm is demonstrated and it is discovered that the extracellular h(2)o(2) molecule released per ischemic cell is 2.7-times of that of a normal cell. | 2011 | 21234479 |
| locations of the motor endplate band and motoneurons innervating the sternomastoid muscle in the rat. | sternocleidomastoid (scm) is a long muscle with two bellies, sternomastoid (sm) and cleidomastoid (cm) in the lateral side of the neck. it has been widely used as muscle and myocutaneous flap for reconstruction of oral cavity and facial defects and as a candidate for reinnervation studies. therefore, exact neuroanatomy of the scm is critical for guiding reinnervation procedures. in this study, sm in rats were investigated to document banding pattern of motor endplates (meps) using whole-mount ac ... | 2010 | 21235005 |
| determination of sphingomyelinase-d activity of loxosceles venoms in sphingomyelin/cholesterol liposomes containing horseradish peroxidase. | based on degradation of sphingomyelin/cholesterol liposomes containing entrapped horseradish peroxidase, we evaluated the sphingomyelinase-d (smase-d) activity of scorpion, spider and snake venoms by monitoring spectrophotometrically the product of oxidation of hrp released. the results indicate that loxosceles crude venoms (loxosceles intermedia, loxosceles laeta, loxosceles gaucho and loxosceles similis) displayed smase-d activity in a concentration-dependent manner. furthermore, this activity ... | 2011 | 21236288 |
| detection and comparison of protein-dna interactions using dna-bind plate and horseradish peroxidase-based colorimetric assay. | we describe a procedure for detection and comparison of protein-dna interactions using dna-bind plate and horseradish peroxidase (hrp)-based colorimetric assay. amino-modified oligonucleotide was covalently immobilized on the surface of dna-bind plate. after the complementary oligonucleotide was annealed, the plate was incubated with protein to allow sequence-specific dna binding. primary antibody and hrp-labeled secondary antibody were then employed, and colorimetric assay was conducted before ... | 2011 | 21237130 |
| gold nanoparticles based chemiluminescent resonance energy transfer for immunoassay of alpha fetoprotein cancer marker. | in this paper, we report a new strategy of chemiluminescence resonance energy transfer (cret) by using gold nanoparticles (aunps) as efficient long-range energy acceptor in sandwich immunoassays. in the design of cret system, we chose the highly sensitive chemiluminescence (cl) reaction of luminol and hydrogen peroxide catalysed by horseradish peroxidase (hrp) because the cl spectrum of luminol (λ(max) 425 nm) partially overlaps with the visible absorption bands of aunps. on the basis of cret st ... | 2011 | 21237316 |
| covalent immobilization of horseradish peroxidase via click chemistry and its direct electrochemistry. | a simple and versatile approach for covalent immobilization of redox protein on solid surface via self-assembled technique and click chemistry is reported. the alkynyl-terminated monolayers are obtained by self-assembled technique, then, azido-horseradish peroxidase (azido-hrp) was covalent immobilized onto the formed monolayers by click reaction. the modified process is characterized by reflection absorption infrared spectroscopy (rair), surface-enhanced raman scattering spectroscopy (sers) and ... | 2011 | 21238725 |
| multiplexed sensing based on brownian relaxation of magnetic nanoparticles using a compact ac susceptometer. | a novel multiplexed sensing scheme based on the measurement of the magnetic susceptibility of the affinity captured target molecules on magnetic nanoparticles in liquid suspension is proposed. the ac magnetic susceptibility provides a measurement of brownian relaxation behavior of biomolecules bound to magnetic nanoparticles (mnps) that is related to its hydrodynamic size. a room temperature, compact ac susceptometer is designed and developed to measure complex ac magnetic susceptibility of such ... | 2011 | 21242618 |
| additive effect of dextrans on the stability of horseradish peroxidase. | the influence of various concentration (10, 20, and 30% w/v) of different molar weighted dextrans as additives on the stability of hrp has been studied in aqueous medium. native hrp preparations were formulated with different additives for storage stabilization and better performance at high temperature and ph. the results obtained show a stabilizing effect in the presence of an additive (75 kda dextran). the enzyme with 75 kda dextran (in concentration 10% w/v) showed the highest thermal resist ... | 2011 | 21243417 |
| the fidelity of spin trapping with dmpo in biological systems. | unlike direct esr, spin trap methodology depends on the absolute fidelity of the spin trap reaction. two alternative reactions of 5,5-dimethyl-1-pyrroline n-oxide (dmpo) leading to radical adduct artifacts have been discovered and investigated: inverted spin trapping and the forrester-hepburn nucleophilic mechanism. these two alternate pathways to radical adducts are a combination of one-electron oxidation and nucleophilic addition, in either order. in biological systems, serious artifacts have ... | 2011 | 21246623 |
| a quantitative chemiluminescent assay for analysis of peroxide-based explosives. | a quantitative chemiluminescent method, enabling indirect identification of the peroxide-based explosives tatp (triacetone triperoxide) and hmtd (hexamethylene triperoxide diamine) has been developed. treatment of these compounds with acidic solutions produced peroxides, which were transformed into radical derivatives by horseradish peroxidase (hrp) and then quantified by measuring the light emitted during their oxidation of luminol. the method was first developed in the microplate format and la ... | 2011 | 21249343 |
| two types of very long visual fibers found in the optic lobe of the flesh-fly, boettcherisca peregrina. | horseradish peroxidase (hrp) uptake (through a corneal incision) in photoreceptor cells of the compound eye of boettcherisca peregrina, resulted in the labeling of two types of very long visual fibers. one of them (the long fiber, if) penetrates through the lamina and medulla, and directly terminates within the lobula. the other (the bypass fiber, bpf) terminates in the medulla, like the axons of r7 and r8 photoreceptor cells, but the fibers run a considerably roundabout course. the photorecepto ... | 1987 | 21253764 |
| an electrochemical competitive biosensor for ochratoxin a based on a dna biotinylated aptamer. | ochratoxin a (ota) is one of the most important mycotoxin contaminants of foods, particularly cereals and cereal products, with strict low regulatory levels (of ppb) in many countries worldwide. an electrochemical competitive aptamer-based biosensor for ota is described. paramagnetic microparticle beads (mbs) were functionalized with an aptamer specific to ota, and were allowed to compete with a solution of the mycotoxin conjugated to the enzyme horseradish peroxidase (ota-hrp) and free ota. aft ... | 2011 | 21256729 |
| lactulose as a marker of intestinal barrier function in pigs after weaning. | intestinal barrier function in pigs after weaning is almost exclusively determined in terminal experiments with ussing chambers. alternatively, the recovery in urine of orally administered lactulose can be used to assess intestinal permeability in living animals. this experiment was designed to study the barrier function of the small intestine of pigs over time after weaning. the aim was to relate paracellular barrier function (measured by lactulose recovery in the urine) with macromolecular tra ... | 2011 | 21257783 |
| horseradish peroxidase functionalized fluorescent gold nanoclusters for hydrogen peroxide sensing. | the fluorescence of metal nanoclusters provides an amusing optic feature to be applied in various fields. however, rational design of dual functional fluorescent metal nanoclusters directed by active enzyme with targeted application remains little explored. in this work, we report a new strategy to construct enzyme functionalized fluorescent gold nanoclusters via a biomineralization process for the detection of hydrogen peroxide. horseradish peroxidase (hrp) was used as a model functional templa ... | 2011 | 21261275 |
| toxic effect of terbium ion on horseradish cell. | the toxic effect of terbium (iii) ion on the horseradish cell was investigated by scanning electron microscopy, gas chromatography, and standard biochemical methods. it was found that the activity of horseradish peroxidase in the horseradish treated with 0.2 mm terbium (iii) ion decreased and led to the excessive accumulation of free radicals compared with that in the control horseradish. the excessive free radicals could oxidize unsaturated fatty acids in the horseradish cell and then increase ... | 2011 | 21271297 |
| uniform polymer-protein conjugate by aqueous aget atrp using protein as a macroinitiator. | in situ aqueous activators generated by electron transfer for atom transfer radical polymerization (aget atrp) in air, using an enzyme as a macroinitiator, has been proposed to prepare uniform polymer-protein conjugates with improved stability under adverse conditions. in the first step, an initiator, 2-bromoisobutyryl bromide (bib), was grafted onto the protein surface by reaction with the amino groups. the second step was in situ aget atrp polymerization in air using cubr(2)/1,1,4,7,7-pentamet ... | 2011 | 21277397 |
| eosinophils express muscarinic receptors and corticotropin-releasing factor to disrupt the mucosal barrier in ulcerative colitis. | altered intestinal barrier function has been implicated in the pathophysiology of ulcerative colitis (uc) in genetic, functional, and epidemiological studies. mast cells and corticotropin-releasing factor (crf) regulate the mucosal barrier in human colon. because eosinophils are often increased in colon tissues of patients with uc, we assessed interactions among mast cells, crf, and eosinophils in the mucosal barrier of these patients. | 2011 | 21277851 |
| [the production of hydroxyl radical in hrp-nadh-h2o/o2 systems and its application in chlorobenzene removal]. | the mechanism of radical generation in hrp-nadh-o2/h2o2 systems and state-change of horseradish peroxidase (hrp) was investigated by using esr and uv measurements, and the novel enzyme-coenzymatic systems were performed to degrade chlorobenzene as a non-phenolic persistent organic pollutants. the uv results showed that compound iii was produced from hrp oxidized by hydrogen peroxide with the catalysis of nadh, which would generate hydroxyl radical. the esr results demonstrated the production of ... | 2010 | 21284196 |
| rachiplusia nu larva as a biofactory to achieve high level expression of horseradish peroxidase. | a process based on orally-infected rachiplusia nu larvae as biological factories for expression and one-step purification of horseradish peroxidase isozyme c (hrp-c) is described. the process allows obtaining high levels of pure hrp-c by membrane chromatography purification. the introduction of the partial polyhedrin homology sequence element in the target gene increased hrp-c expression level by 2.8-fold whereas it increased 1.8-fold when the larvae were reared at 27 °c instead of at 24 °c, sum ... | 2011 | 21287234 |
| oligonucleotide probes applied for sensitive enzyme-amplified electrochemical assay of mercury(ii) ions. | we developed a novel electrochemical sensor for hg(2+) detection using two mercury-specific oligonucleotide probes and streptavidin-horseradish peroxidase (hrp) enzymatic signal amplification. the two mercury-specific oligonucleotide probes comprised a thiolated capture probe and a biotinated signal probe. the thiolated capture probe was immobilized on a gold electrode. in the presence of hg(2+), the thymine-hg(2+)-thymine (t-hg(2+)-t) interaction between the mismatched t-t base pairs directed t ... | 2011 | 21288711 |
| crucial roles of ngf in dorsal horn plasticity in partially deafferentated cats. | though exogenous nerve growth factor (ngf) has been implicated in spinal cord plasticity, whether endogenous ngf plays a crucial role has not been established in vivo. this study investigated first the role of endogenous ngf in spinal dorsal horn (dh) plasticity following removal of l1-l5 and l7-s2 dorsal root ganglions (drgs) in cats. co-culture of chick embryo drg with dh condition media, protein band fishing by cells as well as western blot showed that ngf could promote neurite growth in vitr ... | 2011 | 21291350 |
| phosphate buffer effects on thermal stability and h2o2-resistance of horseradish peroxidase. | horseradish peroxidase (hrp) has attracted intense research interest due to its potential applications in biotechnological fields. however, inadequate stability under prevalent conditions such as elevated temperatures and h(2)o(2) exposure, has limited its industrial application. in this study, stability of hrp was investigated in the presence of different buffer systems (potassium phosphate and tris-hcl) and additives. it was shown that the concentration of phosphate buffer severely affects enz ... | 2011 | 21291907 |
| chondrogenesis in injectable enzymatically crosslinked heparin/dextran hydrogels. | in this study, injectable hydrogels were prepared by horseradish peroxidase-mediated co-crosslinking of dextran-tyramine (dex-ta) and heparin-tyramine (hep-ta) conjugates and used as scaffolds for cartilage tissue engineering. the swelling and mechanical properties of these hydrogels can be easily controlled by the dex-ta/hep-ta weight ratio. when chondrocytes were incorporated in these gels, cell viability and proliferation were highest for gels with a 50/50 weight ratio of dex-ta/hep-ta. moreo ... | 2011 | 21291927 |
| hypoxia-induced cellular and vascular changes in the nucleus tractus solitarius and ventrolateral medulla. | major changes in arterial pressure, autonomic, and respiratory activity occur in response to hypoxia. we analyzed structural damage and increased vascular permeability in the ventrolateral medulla and nucleus tractus solitarius, which control autonomic, respiratory, and cardiovascular functions in adult wistar rats subjected to 2 hours of hypoxia (7% oxygen + 93% nitrogen) for up to 14 days after hypoxicexposure. brainstem tissue levels of vascular endothelial growth factor (vegf), nitric oxide ... | 2011 | 21293297 |
| hydrogen peroxide sensing with horseradish peroxidase-modified polymer single conical nanochannels. | inspired from the funtioning and responsiveness of biological ion channels, researchers attempt to develop biosensing systems based on polymer and solid-state nanochannels. the applicability of these nanochannels for detection/sensing of any foreign analyte in the surrounding environment depends critically on the surface characteristics of the inner walls. attaching recognition sites to the channel walls leads to the preparation of sensors targeted at a specific molecule. there are many nanochan ... | 2011 | 21294554 |
| schwann-like mesenchymal stem cells within vein graft facilitate facial nerve regeneration and remyelination. | to compare the ability of bone marrow mesenchymal stem cells (mscs) and transdifferentiated schwann-like mscs (tmscs) in promoting transected facial nerve branches repair in a rabbit model of injury, rabbit tmscs were induced from bone marrow mscs, and schwann cells markers were assessed by western blot analysis. the left facial nerve buccal branch was transected to form a 1-cm gap in 54 rabbits, and the gaps were immediately bridged using autologous vein grafts. animals were then randomly assig ... | 2011 | 21295556 |
| horseradish peroxidase-screen printed biosensors for determination of ochratoxin a. | this work summarizes the manufacturing procedure of horseradish peroxidase (hrp) based biosensors for the determination of the mycotoxin ochratoxin a (ota). the biosensors have been fabricated using the single technology of screen-printing. that is to say, an hrp containing ink has been directly screen-printed onto carbon electrodes, which offers a higher rapidity and simplicity in the manufacturing process of biosensors for ota determination. the formal redox potential of the fe(iii/ii) moiety ... | 2011 | 21296204 |
| effects of asymmetric dimethylarginine on bovine retinal capillary endothelial cell proliferation, reactive oxygen species production, permeability, intercellular adhesion molecule-1, and occludin expression. | asymmetric dimethylarginine (adma), an endogenous competitive inhibitor of nitric oxide synthase, is associated with impaired endothelial dysfunction, such as chronic heart failure, hypertension, diabetes, and pulmonary hypertension. the effects of adma on cell proliferation, reactive oxygen species (ros) production, cell permeability, intercellular adhesion molecule-1 (icam-1), and tight-junction protein occludin levels in bovine retinal capillary endothelial cells (brcecs) were investigated. | 2011 | 21297899 |
| in situ generation of hydrogen peroxide by carbohydrate oxidase and cellobiose dehydrogenase for bleaching purposes. | the carbohydrate oxidase from microdochium nivale (caox), heterologously expressed in aspergillus oryzae, and cellobiose dehydrogenase from myriococcum thermophilum (mtcdh), were assessed for their ability to generate bleaching species at a ph suitable for liquid detergents. the substrate specificities of caox and mtcdh were analyzed on a large variety of soluble and insoluble substrates, using oxygen as an electron receptor. even insoluble substrates like cellulose were oxidized from both caox ... | 2010 | 21298807 |
| flow system for the automatic screening of the effect of phenolic compounds on the luminol-hydrogen peroxide-peroxidase chemiluminescence system. | in this work, an automated flow-based procedure for the screening of the effect of the different phenolic compounds on the chemiluminescence (cl) luminol-hydrogen peroxide-horseradish peroxidase (hrp) system is presented. this procedure involves the combination of multisyringe flow injection analysis (mfsia) and sequential injection analysis (sia) techniques and exploits the ability of the different subgroups of phenols, such as cholorophenols, nitrophenols, methylphenols and polyphenols, to enh ... | 2013 | 21308949 |
| carcinoembryonic antigen admittance biosensor based on au and zno nanoparticles using fft admittance voltammetry. | in this work, a highly sensitive carcinoembryonic antigen fast fourier transform admittance biosensor is introduced. the proposed biosensor is based on bilayer films of zno/au nanoparticles as an immobilization matrix. these layers are prepared by self-assembly and deposition method on a gold electrode surface, respectively. carcinoembryonic antibody (anti-cea) was immobilized on gold nanoparticles and positively charged horseradish peroxidase (hrp) was used to block sites against nonspecific bi ... | 2011 | 21309514 |
| influence of nanoreactor environment and substrate location on the activity of horseradish peroxidase in olive oil based water-in-oil microemulsions. | oxidative enzymatic reactions using horseradish peroxidase (hrp) were carried out in water-in-oil (w/o) microemulsions composed of olive oil/lecithin/1-propanol/water, a model biomimetic system. the substrates used (gallic acid, octyl gallate and 2,2'-azino-bis[3-ethylbenzo-thiazoline-6-sulfonic acid] (abts)) have different hydrophobicities and possible locations in the microemulsion system. hrp reactivity with reference to substrate hydrophobicity and structural characteristics of the microemul ... | 2011 | 21314158 |
| amperometric hydrogen peroxide biosensor based on the immobilization of heme proteins on gold nanoparticles-bacteria cellulose nanofibers nanocomposite. | a novel matrix, gold nanoparticles-bacterial cellulose nanofibers (au-bc) nanocomposite was developed for enzyme immobilization and biosensor fabrication due to its unique properties such as satisfying biocompatibility, good conductivity and extensive surface area, which were inherited from both gold nanoparticles (aunps) and bacterial cellulose nanofibers (bc). heme proteins such as horseradish peroxidase (hrp), hemoglobin (hb) and myoglobin (mb) were successfully immobilized on the surface of ... | 2010 | 21315900 |
| micro-plate chemiluminescence enzyme immunoassay for aflatoxin b1 in agricultural products. | in this work, a micro-plate chemiluminescence enzyme immunoassay by antibody-coated for the determination of aflatoxin b1 (afb1) in agricultural products has been established. aflatoxin b1 antibody (afb1-ab) was adsorbed physically on polystyrene micro-plate hole as solid phase antibody, which took place immunity-reaction between antigen and antibody with afb1 standard solution or samples by direct competition. luminol-hydrogen peroxide chemiluminescence system catalyzed by horseradish peroxidas ... | 2011 | 21315923 |
| degradation of pentachlorophenol by a novel peroxidase-catalyzed process in the presence of reduced nicotinamide adenine dinucleotide. | a novel horseradish peroxidase (hrp)-catalyzed h₂o₂ process in the presence of reduced nicotinamide adenine dinucleotide (nadh) was applied to remove aqueous pentachlorophenol (pcp). parameters (ph, h₂o₂ concentration, hrp activity and nadh dosage) on pcp removal were investigated. it was found that initial 0.05mm pcp was removed by 98% in hrp-nadh-h₂o₂ system at ph 5.0 and 30°c for 1h. addition of o₂ in hrp-nadh-h₂o₂ system enhanced the removal rate of pcp due to promoting hydroxyl radicals (.o ... | 2011 | 21316072 |
| the use of hrp in decolorization of reactive dyes and toxicological evaluation of their products. | this work studied the potential use of horseradish peroxidase (hrp) in the decolorization of the following textile dyes: drimarene blue x-3lr (dmblr), drimarene blue x-bln (dmbbln), drimarene rubinol x-3lr (dmr), and drimarene blue cl-r (rbbr). dyes were individually tested in the reaction media containing 120 mg·l(-1), considering the following parameters: temperature (20-45°c), h(2)o(2) concentration (0-4.44 mmol·l(-1)), and reaction time (5 minutes, 1 and 24 h). the following conditions: 35°c ... | 2011 | 21318147 |
| immobilizing pt nanoparticles and chitosan hybrid film on polyaniline naofibers membrane for an amperometric hydrogen peroxide biosensor. | a convenient and effective way for fabricating amperometric hydrogen peroxide (h2o2) biosensor was designed in this paper. first, the polyaniline (pani) nanofibers membrane with good conductance and high surface area was electropolymerized on a gold electrode surface. then, pt nanoparticle (ptnp) was electrochemically deposited on the pani nanofibers membrane. finally, the hybrid film of gold nanoparticle, chitosan, and horseradish peroxidase (hrp) was cast onto the modified electrode to form a ... | 2011 | 21318624 |
| a simple assay for mammalian spermine oxidase: a polyamine catabolic enzyme implicated in drug response and disease. | spermine oxidase (smo), the most recently characterized polyamine metabolic enzyme, catalyzes the direct back-conversion of spermine to spermidine in an fad-dependent reaction that also yields the byproducts hydrogen peroxide (h(2)o(2)) and 3-aminopropanal. these metabolites, particularly h(2)o(2), have been implicated in cytotoxic cellular responses to specific antitumor polyamine analogs, as well as in the inflammation-associated generation of dna damage. this chapter describes a rapid, sensit ... | 2011 | 21318873 |
| small-scale, high-throughput method for plant n-glycan preparation for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis. | a simple, small-scale, and high-throughput method for preparation of plant n-glycans for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is described. this method entailed the extraction of soluble proteins, pepsin digestion, release of n-glycans by glycopeptidase a, and a three-step chromatographic purification process using cation exchange, anion exchange, and graphitized carbon. homemade minicolumns using commercially available filter unit devices w ... | 2011 | 21320463 |
| interferon-?-induced increases in intestinal epithelial macromolecular permeability requires the src kinase fyn. | interferon-? (ifn?) is an important immunoregulatory cytokine that can also decrease intestinal epithelial barrier function. little is known about the intracellular signalling events immediately subsequent to ifn?/ifn? receptor interaction that mediate increases in epithelial permeability; data that could be used to ablate this effect of ifn? while leaving its immunostimulatory effects intact. this study assessed the potential involvement of src family kinases in ifn?-induced increases in epithe ... | 2011 | 21321534 |
| western blotting. | western blotting is employed for the detection of proteins and other macromolecules immobilized on nitrocellulose membranes. this rapid and sensitive method enables the identification and quantification of a specific protein from cell lysates or a mixture of proteins. following the resolution on denaturing sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis (sds-page), the constituent polypeptides are transferred electrophoretically to a nitrocellulose membrane (1). the membrane is i ... | 2001 | 21331721 |
| selective functionalisation of pdms-based photonic lab on a chip for biosensing. | a comparative study of different approaches for the selective immobilisation of biomolecules on the surface of poly(dimethylsiloxane) (pdms) is reported. the motivation of this work is to set a robust and reliable protocol for the easy implementation of a biosensor device in a pdms-based photonic lab-on-a-chip (phloc). a hollow prism configuration, previously reported for the colorimetric detection of analytes was chosen for this study. here, the inner walls of the hollow prism were initially mo ... | 2011 | 21336349 |
| basic immunocytochemistry for light microscopy. | immunocytochemistry may be defined as the identification of a cell- or tissue-bound antigen in situ, by means of a specific antibody-antigen reaction, tagged microscopically by a visible label. successful immunocytochemistry therefore requires (1) preservation of the antigen in a form that is recognizable by the antibody, (2) a suitable antibody, and (3) an appropriate label. the basic technique was first described by coons and colleagues (1-3), who employed antibody directly labeled with a fluo ... | 2001 | 21340888 |
| fluorescence decrease of conjugated polymers by the catalytic activity of horseradish peroxidase and its application in phenolic compounds detection. | we report the fluorescence decrease of the water-soluble π-π-conjugated polymer poly(2-methoxy-5-propyloxy sulfonate phenylene vinylene, mps-ppv) by the catalytic activity of horseradish peroxidase in the presence of h(2)o(2). mps-ppv acts as a donor substrate in the catalytic cycle of horseradish peroxidase where the electron-deficient enzymatic intermediates compounds i and ii can subtract electrons from the polymer leading to its fluorescence decrease. the addition of phenolic drug acetaminop ... | 2011 | 21341687 |
| the e-loop is involved in hydrogen peroxide formation by the nadph oxidase nox4. | in contrast to the nadph oxidases nox1 and nox2, which generate superoxide (o(2)(·-)), nox4 produces hydrogen peroxide (h(2)o(2)). we constructed chimeric proteins and mutants to address the protein region that specifies which reactive oxygen species is produced. reactive oxygen species were measured with luminol/horseradish peroxidase and amplex red for h(2)o(2) versus l-012 and cytochrome c for o(2)(·-). the third extracytosolic loop (e-loop) of nox4 is 28 amino acids longer than that of nox1 ... | 2011 | 21343298 |
| reversed-phase depletion coupled with hydrophilic affinity enrichment for the selective isolation of n-linked glycopeptides by using click oeg-cd matrix. | selective enrichment of glycopeptides is of great importance for protein glycosylation analysis using mass spectrometry since the signals of glycopeptides could be severely suppressed by the coexisting non-glycosylated peptides in the protein digest. in the present work, a strategy for n-linked glycopeptide enrichment through reversed-phase depletion coupled with hydrophilic affinity enrichment by applying the customized matrix named click oeg-cd is developed. compared with single hydrophilic in ... | 2011 | 21344167 |
| the enzymatic oxidation of graphene oxide. | two-dimensional graphitic carbon is a new material with many emerging applications, and studying its chemical properties is an important goal. here, we reported a new phenomenon--the enzymatic oxidation of a single layer of graphitic carbon by horseradish peroxidase (hrp). in the presence of low concentrations of hydrogen peroxide (∼40 μm), hrp catalyzed the oxidation of graphene oxide, which resulted in the formation of holes on its basal plane. during the same period of analysis, hrp failed to ... | 2011 | 21344859 |
| poly-l-ornithine enhances the uptake of horseradish peroxidase. | poly-l-ornithine (orn) in concentrations of 0.1-0.5% when added to 10-25% concentrations of horseradish peroxidase (hrp) improves significantly the ease of detection of labeled cells with the light microscope. increased uptake of the hrp marker is demonstrated first by the 3-4-fold elevation in the number of retrogradely labeled cells, and second, by the fact that labeled somata are readily detected as early as 5 h and as late as 75-100 h postinjection with orn-hrp. orn-hrp similarly increases t ... | 1978 | 21348348 |
| anterior striatal projections to the globus pallidus, entopeduncular nucleus and substantia nigra in the rat: the gaba connection. | the projections of the head of the striatum of the rat were studied by autoradiography and horseradish peroxidase (hrp) histochemistry. after injections of [3h]-leucine into the head of the striatum, accumulations of autoradiographic grains were observed over the neuropil of the globus pallidus (gp), entopeduncular nucleus (ep) and substantia nigra, pars reticulata (snr). the striatal projections to gp and ep were confirmed using hrp histochemistry. injections of hrp into gp resulted in the appe ... | 1978 | 21348349 |
| transient populations of glial cells in developing rat telencephalon revealed by horseradish peroxidase. | 1978 | 21348364 | |
| enzymatic degradation of multiwalled carbon nanotubes. | because of their unique properties, carbon nanotubes and, in particular, multiwalled carbon nanotubes (mwnts) have been used for the development of advanced composite and catalyst materials. despite their growing commercial applications and increased production, the potential environmental and toxicological impacts of mwnts are not fully understood; however, many reports suggest that they may be toxic. therefore, a need exists to develop protocols for effective and safe degradation of mwnts. in ... | 2011 | 21348486 |
| a possible new relay for tongue thermal sense in the dorsal margin of the trigeminal principal nucleus in rats. | neurons responding to innocuous thermal stimulation of the anterior tongue were newly identified in the dorsal margin of the trigeminal principal nucleus (pv). connections of the dorsal margin of the pv were neuroanatomically identified using wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp) with electrophysiological techniques. injection of wga-hrp into the dorsal margin of the pv demonstrated anterograde labels distributed from the most dorsomedial portion of the posteromedial ... | 2011 | 21354116 |
| horseradish peroxidase compound i as a tool to investigate reactive protein-cysteine residues: from quantification to kinetics. | proteins containing reactive cysteine residues (protein-cys) are receiving increased attention as mediators of hydrogen peroxide signaling. these proteins are mainly identified by mining the thiol proteomes of oxidized protein-cys in cells and tissues. however, it is difficult to determine if oxidation occurs through a direct reaction with hydrogen peroxide or by thiol-disulfide exchange reactions. kinetic studies with purified proteins provide invaluable information about the reactivity of prot ... | 2011 | 21354305 |
| immunohistochemistry of embryo sections. | introductionthis protocol describes how to localize an antigen in cells and tissues using embryo sections attached to glass slides. the method outlined here uses alkaline-phosphatase-coupled secondary antibody; horseradish-peroxidase-coupled secondary antibody can be used as an alternative. | 2008 | 21356664 |
| immunohistochemistry on sections of plant tissues using enzyme-coupled avidin-biotin complex. | introductionlike rna, proteins can be localized in situ on tissue sections. in situ localization or immunohistochemistry provides information on the cellular and subcellular distribution of proteins in different tissues. the technique described in this protocol employs a biotinylated secondary antibody in conjunction with a preformed avidin:biotinylated enzyme complex (abc) for immunohistochemistry. it allows for sensitive detection of antigens while avoiding fluorescent secondary antibodies, wh ... | 2008 | 21356776 |
| nickel-intensified abc-hrp antibody staining of imaginal discs in drosophila. | introductionthis protocol describes a sensitive method for labeling drosophila imaginal disc tissues with a single antiserum. labeling is visualized using a nickel-intensified avidin/biotinylated enzyme complex-horseradish peroxidase (abc-hrp) staining procedure. this method is more sensitive, and has a lower signal-to-noise ratio, than fluorescent labeling. although these methods are useful for visualizing genetic mosaic clones in wandering larval stage imaginal discs and pupal tissues from dro ... | 2007 | 21356991 |
| an experimental study of artificial murine bladder reflex arc established by abdominal reflex. | the neurogenic bladder dysfunction caused by spinal cord injury is difficult to treat clinically. the aim of this research was to establish an artificial bladder reflex arc in rats through abdominal reflex pathway above the level of spinal cord injury, reinnervate the neurogenic bladder and restore bladder micturition. | 2011 | 21362343 |
| a highly efficient and versatile microchip capillary electrophoresis method for dna separation using gold nanoparticle as a tag. | a highly efficient and versatile method for dna separation using au nanoparticles (au nps) as a tag based on microchip capillary electrophoresis (mce) was developed. the thiol-modified dna-binding au nps were utilized as a tag. target dna was sandwiched between au nps and probe dna labeled with horseradish peroxidase (hrp). in electrophoresis separation, the difference in electrophoretic mobility between free probe and probe-target complex was magnified by au nps, which enabled the resulting mix ... | 2011 | 21365742 |
| ph-tunable oxidase-like activity of cerium oxide nanoparticles achieving sensitive fluorigenic detection of cancer biomarkers at neutral ph. | the reliable and sensitive detection of cancer-specific biomarkers is important for the diagnosis and treatment of cancer. hence, detection of these biomarkers has to be reliably and rapidly performed in diverse settings. a limitation of the conventional biomarker-screening method of enzyme-linked immunosorbent assay (elisa) is the employment of labile components, such as hydrogen peroxide and horseradish peroxidase. previously, we reported that nanoceria is able to oxidize various colorimertic ... | 2011 | 21370817 |
| an enhanced elisa based on modified colloidal gold nanoparticles for the detection of pb(ii). | a novel probe based on colloidal gold nanoparticles (aunps) modified with goat anti-mouse igg and horseradish peroxidase (hrp) was synthesized and an enhanced enzyme-linked immunosorbent assay (elisa) based on the probe was developed. in the assay, the synthesized probe is bound with a monoclonal antibody (mcab) which is competitively bound by coated bsa-itcbe-pb(ii) on plate and pb(ii) in samples. the hrp, used here for signal amplification catalytically oxidize the substrate and generate optic ... | 2011 | 21371875 |
| immunoassay based on carbon nanotubes-enhanced elisa for salmonella enterica serovar typhimurium. | among the methods used to detect pathogenic bacteria, enzyme linked immunosorbent assay (elisa) is one of the most widely used techniques in routine sample analysis. for salmonella enterica serovar typhimurium detection, a typical elisa yields a sensitivity of 10(6)-10(7)cfu/ml. to enhance the detection sensitivity, single-walled carbon nanotubes (swcnts) was employed in this study as a labelling platform for antibody and horseradish peroxidase (hrp) co-immobilizing. with high proteins recovery ... | 2011 | 21376561 |
| signal amplification for dna detection based on the hrp-functionalized fe3o4 nanoparticles. | an electrochemical approach for the sensitive detection of sequence-specific dna has been developed. horseradish peroxidase (hrp) assembled on the fe(3)o(4) nanoparticles (nps) were utilized as signal amplification sources. high-content hrp was adsorbed on the fe(3)o(4) nps via layer-by-layer (lbl) technique to prepare hrp-functionalized fe(3)o(4) nps. signal probe and diluting probe were then immobilized on the hrp-functionalized fe(3)o(4) nps through the bridge of au nps. thereafter, the resul ... | 2011 | 21376984 |
| carbon nanotube-based symbiotic coaxial nanocables with nanosilica and nanogold particles as labels for electrochemical immunoassay of carcinoembryonic antigen in biological fluids. | a feasible and practicable amperometric immunoassay strategy for sensitive screening of carcinoembryonic antigen (cea) in human serum was developed using carbon nanotube (cnt)-based symbiotic coaxial nanocables as labels. to construct such a nanocable, a thin layer of silica nanoparticles was coated on the cnt surface by sonication and sol-gel methods, and then colloidal gold nanoparticles were assembled on the amino-functionalized sio(2)/cnts, which were used for the label of horseradish peroxi ... | 2011 | 21376985 |
| highly sensitive electrochemical label-free aptasensor based on dual electrocatalytic amplification of pt-aunps and hrp. | in this work, a label-free electrochemical aptamer-based sensor (aptasensor) was constructed on account of the direct immobilization of redox probes on an electrode surface. for this proposed aptasensor, a gold nanoparticles (aunps)-coated electrode was firstly modified with redox probes-nickel hexacyanoferrates nanoparticles (nihcfnps) through chemisorption and electrostatic adsorption. then, platinum-gold alloy nanoparticles (pt-aunps) and horseradish peroxidase (hrp) were respectively assembl ... | 2011 | 21380419 |
| a novel and practical method using hrp-polymer conjugate and microwave treatment for visualization of 2 antigens raised from the same or different species in paraffin-embedded tissues. | recent advances in imaging systems have improved the ability to simultaneously visualize expression of multiple antigens in a single tissue section. in most instances, fluorescence microscopy, modern imaging systems, electronic scanners, and expensive reagents are required to achieve these results. using conventional methodologies, we have developed an inexpensive and straightforward method for visualizing 2 antigens on a single formalin-fixed paraffin-embedded tissue section. the protocol combi ... | 2011 | 21386705 |
| graphene-assisted dual amplification strategy for the fabrication of sensitive amperometric immunosensor. | a sensitive electrochemical immunosensor with graphene-assisted signal amplification has been developed. in order to construct the base of the immunosensor, a novel hybrid architecture was initially fabricated by combining poly (diallyldimethylammonium chloride) functionalized graphene nanosheets (pdda-g) and gold nanoparticles (aunps) via a simple sonication-induced assembly. the formed hybrid architecture provided an effective matrix for antibody immobilization with good stability and bioactiv ... | 2011 | 21388800 |