Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| dna aptamer folding on magnetic beads for sequential detection of adenosine and cocaine by substrate-resolved chemiluminescence technology. | we have developed a new aptamer-based chemiluminescence (cl) biosensing platform for the sequential detection of two small molecules as exemplified by adenosine and cocaine. each biosensing platform comprises nh(2)-functionalized capture dna immobilized on magnetic beads; this can hybridize with one end of the aptamer. a corresponding reporter dna probe labeled with either digoxigenin or biotin on its 5'-terminus recognizes the other end of the aptamer. the target compounds adenosine or cocaine ... | 2010 | 20652180 |
| phospholipid-templated silica nanocapsules as efficient polyenzymatic biocatalysts. | solid polyenzymatic biocatalysts have been designed by combining two immobilized enzymes, the first one allowing the in situ generation of h(2)o(2) from air and the second one performing an oxidation reaction. the in situ h(2)o(2) generation system is based on the reaction of glucose with air using a glucose oxidase (gox). the optimization of the encapsulation of gox into phospholipids-templated silica capsules (nps) was performed. a bienzymatic system made of gox and horseradish peroxidase (hrp ... | 2010 | 20657927 |
| structural basis for the decrease in the outward potassium channel current induced by lanthanum. | the current of the outward k+ channel in the cell of horseradish treated with la3+ and the direct interaction between la3+ and the k+ channel protein were investigated using the whole-cell patch-clamp technique, molecular dynamics simulation, and quantum chemistry calculation methods. it was found for the first time that la3+ decreases the current of the k+ channel in the horseradish mesophyll cell. the decrease results from the formation of a coordination bond and hydrogen bond between la3+ and ... | 2010 | 20658160 |
| towards a high potential biocathode based on direct bioelectrochemistry between horseradish peroxidase and hierarchically structured carbon nanotubes. | adsorption of horseradish peroxidase (hrp) on graphite rod electrodes sequentially modified with carbon microfibers (cmf) carrying carbon nanotubes in a hierarchically structured arrangement and finally pyrene hexanoic acid (pha) for improving hydrophilicity of the electrode surface is the basis for the direct bioelectrocatalytic reduction of h(2)o(2) at potentials as high as about +600 mv. the high-potential direct bioelectrocatalytic reduction of h(2)o(2) is implying a direct bioelectrochemica ... | 2010 | 20661521 |
| electrochemical immunosensor for norethisterone based on signal amplification strategy of graphene sheets and multienzyme functionalized mesoporous silica nanoparticles. | norethisterone is one kind of widely used anabolic steroid hormones which can help to promote livestock growth and sometime has been illegally used for livestock breeding. the residues of norethisterone in animal food will harm people's health, therefore, it has been banned to use for the growth promotion purposes in livestock. in this study, amino-group functionalized mesoporous silica nanoparticles (msn) were prepared and used to immobilize au nanoparticles, which was further utilized for the ... | 2010 | 20663658 |
| layer-by-layer assembly of bi-protein/layered double hydroxide ultrathin film and its electrocatalytic behavior for catechol. | this paper reports the fabrication of a bi-protein/layered double hydroxide (ldh) ultrathin film in which hemoglobin (hb) and horseradish peroxidase (hrp) molecules were assembled alternately with ldh nanosheets via the layer-by-layer (lbl) deposition technique, and its electrocatalytic performances for oxidation of catechol were demonstrated. the results of xrd indicate that the hb-hrp/ldh ultrathin film possesses a long range stacking order in the normal direction of the substrate, with the tw ... | 2010 | 20678920 |
| feeding on poplar leaves by caterpillars potentiates foliar peroxidase action in their guts and increases plant resistance. | peroxidases (pods) are believed to act as induced and constitutive defenses in plants against leaf-feeding insects. however, little work has examined the mode of action of pods against insects. putative mechanisms include the production of potentially antinutritive and/or toxic semiquinone free radicals and quinones (from the oxidation of phenolics), as well as increased leaf toughness. in this study, transgenic hybrid poplar saplings (populus tremula × populus alba) overexpressing horseradish p ... | 2010 | 20680646 |
| purification of peroxidase from horseradish (armoracia rusticana) roots. | peroxidase (ec 1.11.1.7) from horseradish ( armoracia rusticana ) roots was purified using a simple, rapid, three-step procedure: ultrasonication, ammonium sulfate salt precipitation, and hydrophobic interaction chromatography on phenyl sepharose cl-4b. the preparation gave an overall yield of 71%, 291-fold purification, and a high specific activity of 772 u mg(-1) protein. sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the purified enzyme was homogeneous and had a molec ... | 2010 | 20681636 |
| long-range electron transfer in recombinant peroxidases anisotropically orientated on gold electrodes. | long-range electron transfer (et) in horseradish peroxidase (hrp) was studied with a wild-type recombinant form of hrp, rhrp, and recombinant forms containing histidine and cysteine tags at gln1, asn57, asn189, or ser309 amino acid residues of the protein. chemisorption of the enzyme onto the au electrodes through the tags introduced in different positions of the protein surface provided anisotropic orientations of the rhrps on the au surface, which allowed a restricted "rotation" of the rhrp mo ... | 2010 | 20683517 |
| rapid quantification of melamine in milk using competitive 1,1'-oxalyldiimidazole chemiluminescent enzyme immunoassay. | a novel competitive 1,1'-oxalyldiimidazole (odi) chemiluminescent enzyme immunoassay (cleia) was developed as a method for rapid and simple screening of melamine in milk. fat existing in milk acts as an inhibitor in the competitive binding interaction of melamine and anti-melamine in the presence of melamine-conjugated horseradish peroxidase. thus, the calibration curve and sensitivity of competitive odi cleia for the quantification of melamine in fat free milk were wider and better than those i ... | 2010 | 20683522 |
| in situ electrochemical imaging of membrane glycan expression on micropatterned adherent single cells. | a scanning electrochemical microscopic (secm) method for in situ imaging of four types of membrane glycan motifs on single adherent cells was proposed using bgc-823 human gastric carcinoma (bgc) cells as the model. these adherent cells were first micropatterned in the microwell of poly(dimethylsiloxane) membrane for precisely controlling the localized surface interaction, and the membrane glycans were then specifically recognized with corresponding lectins labeled with horseradish peroxidase (hr ... | 2010 | 20684525 |
| direct electrochemistry and electrocatalysis of horseradish peroxidase with hyaluronic acid-ionic liquid-cadmium sulfide nanorod composite material. | a new composite material consisted of hyaluronic acid (ha), ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]bf(4)) and cadmium sulfide (cds) nanorod was fabricated and used for the immobilization of horseradish peroxidase (hrp) on the surface of a carbon ionic liquid electrode (cile), which was prepared with 1-ethyl-3-methyl-imidazolium ethylsulphate ([emim]etoso(3)) as modifier. spectroscopic results indicated that hrp remained its native structure in the composite film. based ... | 2010 | 20685416 |
| a simple and rapid competitive enzyme-linked immunosorbent assay (celisa) for high-throughput measurement of secretory immunoglobulin a (siga) in saliva. | a simple competitive enzyme-linked immunosorbent assay (celisa) was established for rapid measurement of secretory immunoglobulin a (siga) in saliva. the method was based on competitive reaction between the immobilized iga and free iga in the solution for the limited amount of horseradish peroxidase-conjugated rabbit anti-human iga. in comparison with the conventionally used sandwich elisa, the celisa is simpler, low-cost, and shows better reproducibility since it is not affected by the variatio ... | 2010 | 20685490 |
| ultrasensitive immunosensor for the detection of cancer biomarker based on graphene sheet. | an ultrasensitive electrochemical immunosensor based on graphene sheet (gs) has been developed. gs was used to immobilize mediator thionine (th), horseradish peroxidase (hrp) and secondary anti-prostate-specific antigen (psa) antibody (ab2) and the resulting nanostructure (gs-th-hrp-ab2) was used as the label for the immunosensor. with primary anti-psa antibody (ab1) also immobilized onto the gs, the immunosensor displayed a wide range of linear response (0.002-10 ng/ml), low detection limit (1 ... | 2010 | 20688509 |
| neural-specific α3-fucosylation of n-linked glycans in the drosophila embryo requires fucosyltransferase a and influences developmental signaling associated with o-glycosylation. | addition of fucose (fuc) to glycoprotein n-linked glycans or in o-linkage directly to ser/thr residues modulates specific cell-cell interactions and cell signaling events. vertebrates and invertebrates add fuc in α6-linkage to the reducing terminal n-acetylglucosamine residue of n-glycans. in drosophila and other invertebrates, fuc can also be added in α3-linkage to the same residue. these difucosylated n-glycans are recognized by anti-horseradish peroxidase (anti-hrp) antisera, providing a well ... | 2010 | 20688784 |
| hematin is an alternative catalyst to horseradish peroxidase for in situ hydrogelation of polymers with phenolic hydroxyl groups in vivo. | hematin, an iron-containing porphyrin used in the management of porphyria attacks, was evaluated as an alternative catalyst to horseradish peroxidase (hrp) for in situ gelation of polymers with phenolic hydroxyl (ph) moieties in vivo. an aqueous solution of gelatin derivative with ph moieties was gellable in the presence of both hematin and h2o2. a total of 98.6% adhesion of l929 fibroblast cells 4 h after seeding and their similar morphology to those on substrate coated with unmodified gelatin ... | 2010 | 20690724 |
| reagentless biosensor for hydrogen peroxide based on self-assembled films of horseradish peroxidase/laponite/chitosan and the primary investigation on the inhibitory effect by sulfide. | horseradish peroxidase (hrp) was successfully incorporated into the laponite/chitosan (chit)-modified glassy carbon electrode (gce) using step-by-step self-assembly. the self-assembly processes were monitored by x-ray photoelectron spectroscopy (xps) and electrochemical impedance spectra (eis). direct electrochemistry and electrocatalysis of the self-assembled hrp were investigated. cyclic voltammetry of hrp/laponite/chit/gce, in anaerobic phosphate buffer solution (0.025 m pbs, ph 7.0), display ... | 2010 | 20692151 |
| spectrophotometric quantification of horseradish peroxidase with o-phenylenediamine. | the assay conditions for the spectrophotometric quantification of horseradish peroxidase (hrp) with the chromogenic substrate o-phenylenediamine (opd) at 25°c in the presence of hydrogen peroxide (h(2)o(2)) were optimized. with [opd](0)=3.14 mm and [h(2)o(2)](0)=80 μm at ph 7.2, the initial formation of only one of the possible reaction products and intermediates, 2,3-diaminophenazine (dap, λ(max)=417 nm), was observed. the rate of dap formation during the first 30 min of reaction was followed s ... | 2010 | 20692226 |
| single-cell detection using a thin film transistor photosensor with micro-partitions. | a thin film transistor (tft) photosensor was applied to single-cell detection by identifying cell surface molecules based on chemiluminescence. micro-partitions were directly fabricated on the tft photosensor surface by photolithography. the surface of each pixel was surrounded by 25 μm-height partitions, forming areas of approximately 30 μm × 30 μm for cell entrapment and photosensing. visualization of individual jm cells, stained with mouse anti-human cd8 igg1 primary antibody and horseradish ... | 2010 | 20694269 |
| detection of quorum sensing molecules in burkholderia cepacia culture supernatants with enzyme-linked immunosorbent assays. | the burkholderia cepacia complex (bcc) employs a quorum sensing (qs) mechanism which is a cell density-dependent bacterial communication system to regulate certain gene expressions. as with many other gram-negative bacteria, burkholderia cepacia species use (n-acyl-)homoserine lactones (ahls or hsls) as signalling molecules. because of the essential role of qs in bacterial behavior, the aim of this study was to demonstrate the applicability of our in-house-developed enzyme-linked immunosorbent a ... | 2010 | 20694722 |
| [treatment of spinal cord injury by mild hypothermia combined with bone marrow mesenchymal stem cells transplantation in rats]. | bone marrow mesenchymal stem cells (bmscs) play an important role in repairing nerve injury, meanwhile external temperature has significant effect on bmscs transplantation, proliferation, and differentiation. to investigate the effect of bmscs transplantation and mild hypothermia on repair of rat spinal cord injury (sci). | 2010 | 20695375 |
| quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification with self-produced coreactant from oxygen reduction. | a highly sensitive competitive immunosensor based on the electrochemiluminescence (ecl) of quantum dots (qds) was proposed by coupling with an enzymatic amplification. the fabrication process of the immunosensor was traced with atomic force microscopic images and electrochemical impedance spectra. the strong cathodic ecl emission of the immobilized qds could be detected at a relatively low emission potential. the reduction of dissolved oxygen during the cathodic process provided a self-produced ... | 2010 | 20695454 |
| immunohistochemical expression of cd3, cd20, cd45, cd68 and bcl-2 in oral squamous cell carcinoma. | to study the main processes involved in oral squamous cell carcinoma (oscc): apoptosis and inflammation. | 2010 | 20701075 |
| development of a novel antioxidant assay technique based on g-quadruplex dnazyme. | study on antioxidants' radical scavenging processes and antioxidant capabilities is important for understanding the protective role of antioxidants against oxidative damages associated with some chronic diseases and food degradation. traditional methods to monitor the radical scavenging by antioxidant require expensive instrument and sophisticated synthesis process. herein, we report a novel, simple, colorimetric dnazyme-based method to detect radical-scavenging capacity of antioxidant. in this ... | 2010 | 20702079 |
| decolorization of naphthol blue black using the horseradish peroxidase. | this study evaluates the potential of the enzyme horseradish peroxidase in the decolorization of one common industrial azo dye, naphthol blue black. studies are carried out to understand the process parameters such as ph, temperature and reaction time. the enzymatic decolorization of the dye was examined by uv-vis spectrophotometer and lc-ms measurements. temperature and ph conditions were optimized for obtaining high azo-dye decolorization. azo-dye removal at a ph range 4-6 was found to be the ... | 2011 | 20703828 |
| expression of the carbohydrate recognition domain of fimh and development of a competitive binding assay. | uropathogenic escherichia coli (upec) is the primary cause of urinary tract infections (utis). in the first step of this infective process, the virulence factor fimh located on type 1 pili allows upec to specifically adhere to oligosaccharides, which are part of glycoproteins on the urinary bladder mucosa. this initial step prevents the clearance of e. coli from the urinary tract and enables the invasion of the host cells. because fimh antagonists can block this interaction, they exhibit a promi ... | 2010 | 20705050 |
| trace detection of picloram using an electrochemical immunosensor based on three-dimensional gold nanoclusters. | picloram, a herbicide widely used for broadleaf weed control, is persistent and mobile in soil and water with adverse health and environmental effects. it is important to develop a sensitive method for accurate detection of trace picloram in the environment. in this article, a type of ordered three-dimensional (3d) gold (au) nanoclusters obtained by two-step electrodeposition using the spatial obstruction/direction of the polycarbonate membrane is reported. bovine serum albumin (bsa)-picloram wa ... | 2010 | 20709012 |
| the role of stiffness of gelatin-hydroxyphenylpropionic acid hydrogels formed by enzyme-mediated crosslinking on the differentiation of human mesenchymal stem cell. | we report the stimulation of neurogenesis and myogenesis of human mesenchymal stem cells (hmscs) on the surfaces of biodegradable hydrogels with different stiffness. the hydrogels were composed of gelatin-hydroxyphenylpropionic acid (gtn-hpa) conjugate were formed using the oxidative coupling of phenol moieties catalyzed by hydrogen peroxide (h(2)o(2)) and horseradish peroxidase (hrp). the storage modulus of the hydrogels was readily tuned from 600 to 12800 pa. it was found that the stiffness of ... | 2010 | 20709390 |
| modification of the heme active site to increase the peroxidase activity of thermophilic cytochrome p450: a rational approach. | the site specific mutants of the thermophilic p450 (p450 175a1 or cyp175a1) were designed to introduce residues that could act as acid-base catalysts near the active site to enhance the peroxidases activity. the leu80 in the distal heme pocket of cyp175a1 was located at a position almost equivalent to the glu183 that is involved in stabilization of the ferryl heme intermediate in chloroperoxidase (cpo). the leu80 residue of cyp175a1 was mutated with histidine (l80h) and glutamine (l80q) that cou ... | 2010 | 20709408 |
| immobilization of horseradish peroxidase on nanoporous copper and its potential applications. | nanoporous copper (npc) with a pore size of 100-200 nm was prepared by simply dealloying al(60)cu(40) alloy in a 5 wt.% hcl solution. the npc was characterized by scanning electron microscopy and nitrogen adsorption techniques. horseradish peroxidase (hrp) was immobilized on npc by adsorption. compared with free enzyme, the thermal stability of the immobilized enzyme was greatly improved due to the multiple attachments between the enzyme molecule and the npc surface. after 2h incubation at 50 de ... | 2010 | 20709542 |
| oxidation reactions mediated by single-walled carbon nanotubes in aqueous solution. | how single-walled carbon nanotubes (swcnt) function in redox reactions may be related to their behaviors in the induction of oxidative stress. herein, oxidation of several biologically relevant reducing agents in the presence of swcnt was studied in aqueous solutions. the selected reductants included a common indicator for intracellular reactive oxygen species (ros) (2,7-dichlorodihydrofluorescein), small antioxidants (vitamin c, trolox, and cysteine), and a high-molecular-weight ros scavenger ( ... | 2010 | 20715868 |
| horseradish peroxidase-functionalized pt hollow nanospheres and multiple redox probes as trace labels for a sensitive simultaneous multianalyte electrochemical immunoassay. | a novel strategy for a simultaneous multianalyte electrochemical immunoassay is proposed using thionine-labeled anti-afp and ferrocene-labeled anti-cea as tracers; each biorecognition event yielded a distinct voltammetric peak, whose position and size reflected the identify and level of the corresponding antigen. | 2010 | 20721401 |
| phytoremediation of pharmaceuticals--preliminary study. | phytoremediation of selected pharmaceuticals (diclofenac, ibuprofen, and acetaminophen) using armoracia rusticana and linum usitatissimum cell cultures and by hydroponically cultivated lupinus albus, hordeum vulgaris, and phragmites australis plants in laboratory conditions is described. during in vitro experiments, the best results for acetaminophen were achieved using armoracia rusticana hairy root cultures, where 100% of the starting amount was removed from the media during eight days. total ... | 2010 | 20734624 |
| advent of innovative chemiluminescent enzyme immunoassay. | using 1,1'-oxalyldiimidazole (odi) chemiluminescence detection, a new chemiluminescent enzyme immunoassay (cleia) was developed to quantify prostate specific antigen (psa) in human serum. the results observed in odi cleia were compared with those obtained in commercially available enzyme linked immunosorbent assay (elisa), fluorescence enzyme immunoassay (feia), and luminol cleia. psa complex-conjugated horseradish peroxidase (hrp) was formed from one-step sandwich immunoreaction of psa, psa pri ... | 2010 | 20739174 |
| qsar-assisted design of an environmental catalyst for enhanced estrogen remediation. | a quantitative structure-activity relationship (qsar) was used to streamline re-design of a model environmental catalyst, horseradish peroxidase (hrp), for enhanced reactivity towards a target pollutant, steroid hormone 17β-estradiol. this qsar, embodying relationship between reaction rate and intermolecular binding distance, was used in silico to screen for mutations improving enzyme reactivity. eight mutations mediating significant reductions in binding distances were expressed in saccharomyce ... | 2010 | 20797763 |
| formation of surface traps on quantum dots by bidentate chelation and their application in low-potential electrochemiluminescent biosensing. | bidentate chelation, meso-2,3-dimercaptosuccinic acid (dmsa), was used as a stabilizer for the synthesis of cdte quantum dots (qds). the bidentate chelate qds, characterized with ft-ir, pl, and uv/vis spectroscopy; element analysis; and high-resolution transmission electron microscope, exhibited surface traps due to the large surface/volume ratio of qd particle and the steric hindrance of the dmsa molecule. the unpassivated surface of the qds produced a narrower band gap than the core and electr ... | 2010 | 20799302 |
| polystyrene microspheres based sandwich immunosensor using cdte nanoparticles amplification and ultrasensitive flow-injection chemiluminescence detection. | in this paper we propose a specific sandwich immunoassay method for human-immunoglobulin g (higg). this immunoassay protocol takes advantage of sandwich binding of primary and secondary antibodies for increased specificity. polystyrene microspheres (ps) serve as immobilizing support, site for sandwich immunoassay and then subsequently used for chemiluminescence (cl) detections. in this sandwich immunoassay, ps microspheres were modified with the primary anti-higg (ab1) via electrostatic interact ... | 2010 | 20801005 |
| direct electrochemistry-based hydrogen peroxide biosensor formed from single-layer graphene nanoplatelet-enzyme composite film. | a novel electrochemical sensing system for direct electrochemistry-based hydrogen peroxide biosensor was developed that relied on the virtues of excellent biocompatibility, conductivity and high sensitivity to the local perturbations of single-layer graphene nanoplatelet (slgnp). to demonstrate the concept, the horseradish peroxidase (hrp) enzyme was selected as a model to form the slgnp-tpa (tetrasodium 1,3,6,8-pyrenetetrasulfonic acid)-hrp composite film. the single-layer graphene composite fi ... | 2010 | 20801339 |
| automated chemiluminescent dual-analyte immunoassay based on resolved immunosensing channels. | a novel system of series-wound immunosensing channels (swic) was proposed for automated chemiluminescent (cl) dual-analyte immunoassay by immobilizing respectively different capture antibodies on the inner walls of series-wound glass channels. this system could use a single enzyme as label to perform multiplex immunoassay in one fluid way. using alpha-fetoprotein (afp) and carcinoembryonic antigen (cea) as model analytes, the mixture including afp, horseradish peroxidase (hrp)-labeled anti-afp a ... | 2010 | 20801356 |
| a secondary antibody format chemiluminescence immunoassay for the determination of estradiol in human serum. | a competitive immunoassay for estradiol (e2) based on secondary antibody format was established. the donkey anti-rabbit igg was used as the secondary antibody to coat micro-plates, and the horseradish peroxidase (hrp)-luminol-h(2)o(2) chemiluminescent system with high sensitivity was chosen as the detection system. the addition of sodium trichloroacetate (ccl(3)coona) in the enzyme buffer as a replaceable packing material can realize directly analysis of e2 in human serum without extraction, whi ... | 2010 | 20801358 |
| a highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/au nanocomposite for horseradish peroxidase immobilization and biosensing. | a highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/au (pedot-pss/au) nanocomposite was prepared via one-step chemical synthesis and the matrix was characterized by uv-vis spectroscopy (uv-vis), fourier transform infrared spectroscopy (ft-ir), scanning electron microscope (sem) and transmission electron microscope (tem). due to the excellent aqueous compatibility and biocompatibility, the pedot/pss-au nanocomposite can be used as biomaterial for enzymes immobilization. ... | 2010 | 20801365 |
| in vitro studies of dna damage caused by tricyclic antidepressants: a role of peroxidase in the side effects of the drugs. | studies show that tricyclic antidepressants prescribed for migraines, anxiety, and child enuresis have numerous adverse effects in living cells. one of the undesired outcomes observed under treatment with these drugs is dna damage. however, the mechanisms underlying damage have yet to be elucidated. we performed in vitro studies of the dna damage caused by four tricyclic antidepressants: imipramine, amitriptyline, opipramol, and protriptyline. we focused particularly on the dna damage aided by p ... | 2010 | 20804147 |
| lipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen cryopreserved in extenders with antioxidants. | the objective of this study was to evaluate the effect of addition of the antioxidants trolox and catalase to a ram semen cryopreservation extender on lipid peroxidation and hydrogen peroxide generation on the extender and in the thawed semen. semen was collected from 23 santa inês rams (one ejaculate per ram) and diluted at 32°c to a concentration of 400×10⁶ cells/ml in one of the following solution: tris-egg yolk extender (control), or the same extender supplemented with either 50μm trolox/10⁸ ... | 2010 | 20813469 |
| biosynthesis and characterization of organosoluble conjugated poly(2-aminofluorene) with the pyrazine bridged. | in this paper, the results on horseradish peroxidase (hrp)-catalyzed oxidative polymerization of amine-functionalized fluorene monomer, 2-amino fluorene (af), are reported. the resulting polymer exhibits an exciting molecular structure and spectral properties. ft-ir and nmr studies show that the two fluorene units come together by forming an intermediate six-membered pyrazine ring and these formed dimeric units are linked to the each other through c-c couplings to produce fully conjugated polyme ... | 2010 | 20815396 |
| a disposable immunosensor for shigella flexneri based on multiwalled carbon nanotube/sodium alginate composite electrode. | a novel shigella flexneri immunosensor based on horseradish peroxidase-labeled antibodies to s. flexneri (hrp-anti-s. flexneri) immobilized by physical adsorption on the multiwalled carbon nanotube (mwcnt)/sodium alginate (sa) composite modified screen-printed electrode surface was successfully fabricated. in this strategy, mwcnt/sa biocomposite acted as the matrix to adsorb and immobilize hrp-anti-s. flexneri. the modified electrodes were characterized with an atomic force microscope and cyclic ... | 2011 | 20816661 |
| tanshinone iia protects the human blood-brain barrier model from leukocyte-associated hypoxia-reoxygenation injury. | to investigate the in vitro effect of tanshinone iia on leukocyte-associated hypoxia-reoxygenation injury of human brain-blood barrier (bbb), we established the bbb model by culturing purified primary human brain microvascular endothelial cells (hbmvec) to confluence on cell culture insert. bbb was identified by tight junction, transendothelial electrical resistance (teer) and the permeability of bbb to horseradish peroxidase (hrp). the effect of tanshinone iia on the permeability of bbb was tes ... | 2010 | 20826144 |
| favorably orienting recombinant proteins to develop amperometric biosensors to diagnose chagas' disease. | clinical immunoassays often display suitable sensitivity but some lack of specificity or vice versa. as a trade-off between specificity improvement and sensitivity loss, biosensors were designed to perform indirect immunoassays with amperometric detection using tailor-made chimeric receptors to react with the analyte, specific anti-trypanosoma cruzi immunoglobulin g (igg). recombinant chimeras were designed to favor their oriented covalent attachment. this allows the chimeras to properly expose ... | 2011 | 20828530 |
| fluorometric assay of oleate-activated phospholipase d isoenzyme in membranes of rat nervous tissue and human platelets. | phospholipase d plays a key role in the biosynthesis of phosphatidic acid, a second messenger involved in essential cellular processes. oleate-activated phospholipase d was the first mammalian phospholipase d isoform to be discovered but is the least known. the study was aimed to test a fluorometric method of assessment of oleate-activated phospholipase d activity in different biological materials. the brain cortex of male wistar rats, cultured rat brain astrocytes, and human platelets were proc ... | 2010 | 20835407 |
| tresyl-based conjugation of protein antigen to lipid nanoparticles increases antigen immunogenicity. | the present studies were aimed at investigating the engineering of nps with protein-conjugated-surfactant at their surface. in order to increase the immunogenicity of a protein antigen, brij 78 was functionalized by tresyl chloride and then further reacted with the primary amine of the model proteins ovalbumin (ova) or horseradish peroxide (hrp). the reaction yielded brij 78-ova and brij 78-hrp conjugates which were then used directly to form np-ova or np-hrp using a one-step warm oil-in-water m ... | 2010 | 20837122 |
| resident macrophages in the cochlear blood-labyrinth barrier and their renewal via migration of bone-marrow-derived cells. | a large population of perivascular cells was found to be present in the area of the blood-labyrinth barrier in the stria vascularis of normal adult cochlea. the cells were identified as perivascular resident macrophages (pvms), as they were positive for several macrophage surface molecules including f4/80, cd68, and cd11b. the macrophages, which were closely associated with microvessels and structurally intertwined with endothelial cells and pericytes, constitutively expressed scavenger receptor ... | 2010 | 20838812 |
| activators generated electron transfer for atom transfer radical polymerization for immunosensing. | a novel and ultrasensitive immunosensing strategy based on activators generated electron transfer for atom transfer radical polymerization (aget atrp) in combination with flow injection chemiluminescent (cl) and electrochemical detection was proposed. the initiator-conjugated polyclone psa antibodies (ab2*), prepared by coupling of n-hydroxysuccinmidyl bromoisobutyrate (initiator) with polyclone psa antibodies (ab2), were immobilized on the substrate surface through sandwiched immunoreactions to ... | 2010 | 20843677 |
| development of ultra-sensitive soybean peroxidase-based cl-elisa for the determination of human thyroglobulin. | serum thyroglobulin (tg) is a main marker of thyroid cancer relapses after total or near-total thyroidectomy of patients with differentiated thyroid carcinoma. in this study, we developed a chemiluminescent enzyme-linked immunosorbent assay (cl-elisa) for detecting tg in human serum. soybean peroxidase (sbp) in combination with 3-(10'-phenothiazinyl)propane-1-sulfonate (sptz) and 4-morpholinopyridine (morph) and horseradish peroxidase (hrp) with p-iodophenol (pip) were used as detection systems ... | 2010 | 20851124 |
| comparison of enzyme-linked immunosorbent assay-based techniques for the detection of antibody to rift valley fever virus in thermochemically inactivated sheep sera. | different enzyme-linked immunosorbent assay (elisa)-based techniques for the detection of antibodies to rift valley fever virus (rvfv) have been developed in recent years, but their diagnostic sensitivity was not directly compared. in addition, their use might still be restricted to high biocontainment facilities when sera to be tested are collected from viremic individuals. in this study, we report on direct comparison of various elisa forms for the detection of anti-rvfv antibody in preinactiv ... | 2010 | 20854023 |
| visualization of microvascular blood flow in mouse kidney and spleen by quantum dot injection with "in vivo cryotechnique". | the "in vivo cryotechnique" (ivct) is a powerful tool to instantly capture blood flow, and all plasma components are well kept in tissue samples. in this study, we injected glutathione (gsh)-coated quantum dots (qds), which emit a 650-nm-fluorescent signal with an ultraviolet excitation, into anesthetized mouse left ventricles, and ivct was performed for kidneys, spleens and livers at 2, 5, 10, 15, 30s or 24h after the qd injection. the frozen tissues were processed to freeze-substitution fixati ... | 2010 | 20858507 |
| a novel homogeneous biotin-digoxigenin based assay for the detection of human anti-therapeutic antibodies in autoimmune serum. | electrochemiluminescence (ecl) assays have been widely used for the detection of anti-therapeutic antibodies (atas) against biotherapeutics. with the discontinuation of bioveris (bv) ecl platform, an alternative technology was needed to replace bv assays to ensure continuous support of multi-year clinical studies. after evaluation of several immunoassay platforms, a novel homogeneous biotin-digoxigenin (dig) based bridging elisa format was selected to develop an anti-rhumabx antibody screening a ... | 2010 | 20868690 |
| angiotensin ii type 1 receptor (at1) signaling in astrocytes regulates synaptic degeneration-induced leukocyte entry to the central nervous system. | astrocytes are the major cellular component of the blood-brain barrier glia limitans and act as regulators of leukocyte infiltration via chemokine expression. we have studied angiotensin-ii receptor type 1 (at1) and related nf-κb signaling in astrocytes. angiotensin ii derives from cleavage of angiotensin i by angiotensin converting enzyme (ace), angiotensin i deriving from angiotensinogen via cleavage by renin. level of expression of ace was slightly increased in transgenic mice that express do ... | 2010 | 20868737 |
| oxidative dechlorination of halogenated phenols catalyzed by two distinct enzymes: horseradish peroxidase and dehaloperoxidase. | the mechanism of the dehalogenation step catalyzed by dehaloperoxidase (dhp) from amphitrite ornata, an unusual heme-containing protein with a globin fold and peroxidase activity, has remarkable similarity with that of the classical heme peroxidase, horseradish peroxidase (hrp). based on quantum mechanical/molecular mechanical (qm/mm) modeling and experimentally determined chlorine kinetic isotope effects, we have concluded that two sequential one electron oxidations of the halogenated phenol su ... | 2011 | 20869943 |
| ultrasensitive electrochemical immunoassay of staphylococcal enterotoxin b in food using enzyme-nanosilica-doped carbon nanotubes for signal amplification. | a new sandwich-type electrochemical immunoassay for ultrasensitive detection of staphylococcal enterotoxin b (seb) in food was developed using horseradish peroxidase-nanosilica-doped multiwalled carbon nanotubes (hrpsicnts) for signal amplification. rabbit polyclonal anti-seb antibodies immobilized on the screen-printed carbon electrode (spce) and covalently bound to the hrpsicnts were used as capture antibodies and detection antibodies, respectively. in the presence of seb analyte, the sandwich ... | 2010 | 20873791 |
| ultrasensitive nucleic acid biosensor based on enzyme-gold nanoparticle dual label and lateral flow strip biosensor. | in this article, we describe an ultrasensitive nucleic acid biosensor (nab) based on horseradish peroxidase (hrp)-gold nanoparticle (au-np) dual labels and lateral flow strip biosensor (lfsb). the results presented here expand on prior work (mao et al., 2009a) by optimizing the preparation of hrp-au-np-dna conjugates. it was found that sodium dodecyl sulfate (sds) and the immobilization sequence of thiolated dna and hrp on the au-np surface played very important roles to improve the sensitivity ... | 2011 | 20875950 |
| a peptide-based material platform for displaying antibodies to engage t cells. | this study investigated a strategy by which antibodies are displayed to engage t cells. a peptide composite containing binding sites was characterized in vitro and explored as an injectable system in vivo. the composite consists of two amphiphilic peptides, aeaeakakaeaeakak (referred to as "eak") and eak appended with six consecutive histidines at the c-terminus ("eakh6"). spectroscopic analysis showed the two peptides integrated into a single structure. prior to combination, conformational anal ... | 2010 | 20880580 |
| ternary surface monolayers for ultrasensitive (zeptomole) amperometric detection of nucleic acid hybridization without signal amplification. | a ternary surface monolayer, consisting of coassembled thiolated capture probe, mercaptohexanol and dithiothreitol, is shown to offer dramatic improvements in the signal-to-noise characteristics of electrochemical dna hybridization biosensors based on common self-assembled monolayers. remarkably low detection limits down to 40 zmol (in 4 μl samples) as well as only 1 cfu escherichia coli per sensor are thus obtained without any additional amplification step in connection to the commonly used hor ... | 2010 | 20883023 |
| potential of melatonin to treat or prevent age-related macular degeneration through stimulation of telomerase activity. | melatonin may play a causal role in the occurrence of age-related macular degeneration (amd). replicative capacity and response to injury in the retinal pigment epithelium (rpe) is compromised during aging. prevention of telomere shortening by antioxidants may be a useful approach for reducing the cumulative effects of oxidative stress in rpe cells. melatonin, a well known antioxidant, which acts advantageously as an amphiphilic agent, may benefit amd patients more than commonly used lipophilic ... | 2010 | 20884126 |
| total low-molecular-weight antioxidants as a summary parameter, quantified in biological samples by a chemiluminescence inhibition assay. | aerobic metabolism requires a complex antioxidative system to balance reactive oxygen species (ros). when in excess, ros disrupt cellular activities and molecular structures. owing to the variety of ros, there are different antioxidative activities that require various tests for their detection. the so-called 'total antioxidative capacity' inhibition assay presented in this paper can be used to quantify the overall activity of low-molecular-weight antioxidants (aos) in biological samples. the as ... | 2010 | 20885374 |
| increased plp1 gene expression leads to massive microglial cell activation and inflammation throughout the brain. | pmd (pelizaeus-merzbacher disease) is a rare neurodegenerative disorder that impairs motor and cognitive functions and is associated with a shortened lifespan. the cause of pmd is mutations of the plp1 [proteolipid protein 1 gene (human)] gene. transgenic mice with increased plp1 [proteolipid protein 1 gene (non-human)] copy number model most aspects of pmd patients with duplications. hypomyelination and demyelination are believed to cause the neurological abnormalities in mammals with plp1 dupl ... | 2010 | 20885931 |
| oxidative metabolism of the anti-cancer agent mitoxantrone by horseradish, lacto-and lignin peroxidase. | mitoxantrone (mh(2)x), an anthraquinone-type anti-cancer agent used clinically in the treatment of human malignancies, is oxidatively activated by the peroxidase/h(2)o(2) enzyme system. in contrast to the enzymatic mechanisms of drug oxidation, the chemical transformations of mh(2)x are not well described. in this study, mh(2)x metabolites, produced by the horseradish, lacto- or lignin peroxidase (respectively hrp, lpo and lip)/h(2)o(2) system, were investigated by steady-state spectrokinetic an ... | 2010 | 20887767 |
| one-chip biosensor for simultaneous disease marker/calibration substance measurement in human urine by electrochemical surface plasmon resonance method. | we have developed a miniaturized electrochemical surface plasmon resonance biosensor for measuring two biomolecules that have very different molecular sizes, one is transferrin (mw=75 kda) as a disease marker protein, the other is creatinine (mw=113) as a calibration marker for the accurate measurement of human urinary samples. the sensor has a pdms based microchannel that is 2 mm wide and 20 μm deep. two gold films were integrated in the microchannel; one was modified with anti-transferrin anti ... | 2010 | 20888212 |
| nanogold-actuated biomimetic peroxidase for sensitized electrochemical immunoassay of carcinoembryonic antigen in human serum. | a new electrochemical immunoassay protocol for the determination of carcinoembryonic antigen (cea) in human serum was developed by means of immobilizing horseradish peroxidase-labeled anti-cea antibodies (hrp-anti-cea) on the nanogold/nano-fe(3)o(4) particles-functionalized polyion complex (pic) membrane. with a one-step immunoassay format, the formed immunocomplex inhibited partly the bioactive center of the immobilized hrp, and decreased the hrp toward the reduction of h(2)o(2). nanogold-actua ... | 2010 | 20888448 |
| visualizing axons in the drosophila central nervous system using immunohistochemistry and immunofluorescence. | how axons in the developing nervous system navigate to their correct targets is a fundamental question in neuroscience. studies of axon guidance in the embryonic central nervous system (cns) of drosophila melanogaster have proved instrumental to the identification of molecules and mechanisms that regulate wiring. the relative simplicity of the drosophila embryonic cns, the advantages of genetic approaches, and the ability to analyze the pathfinding decisions of well-defined axon groups or even i ... | 2010 | 20889700 |
| gold nanoparticles-decorated amine-terminated poly(amidoamine) dendrimer for sensitive electrochemical immunoassay of brevetoxins in food samples. | a sensitive electrochemical immunosensor for the fast screening of brevetoxin b (btx-2) in food samples was developed by means of immobilizing btx-2-bovine serum albumin conjugate (btx-2-bsa) on the gold nanoparticles-decorated amine-terminated poly(amidoamine) dendrimers (aunp-paads). the presence of gold nanoparticles greatly improved the conductivity of the paads, and three-dimensional paads increased the surface coverage of the biomolecules on the electrode. under optimal conditions, three t ... | 2010 | 20926280 |
| projections from the brain to the spinal cord in the mouse. | the cells that project from the brain to the spinal cord have previously been mapped in a wide range of mammalian species, but have not been comprehensively studied in the mouse. we have mapped these cells in the mouse using retrograde tracing after large unilateral fluoro-gold (fg) and horseradish peroxidase (hrp) injections in the c1 and c2 spinal cord segments. we have identified over 30 cell groups that project to the spinal cord, and have confirmed that the pattern of major projections from ... | 2010 | 20936329 |
| immunosensor based on fluorescence quenching matrix of the conducting polymer polypyrrole. | in this study, the combination of autofluorescent proteins and fluorescence quenching polymers was shown to be a design which can increase the selectivity and sensitivity of immunosensors. with this objective, the conducting polymer polypyrrole (ppy) was used as a matrix for immobilization of proteins, which enables biological recognition of the analyte, and as a fluorescence quencher, which increases the selectivity of fluorescence-based detection. in this study, bovine leukemia virus proteins ... | 2010 | 20941482 |
| a voltammetric immunosensor based on nanobiocomposite materials for the determination of alpha-fetoprotein in serum. | the development of a voltammetric immunosensor for determination of alpha-fetoprotein (afp) in serum is presented. elisa assays with voltammetric reading were carried out exploiting the peculiar properties of nanobiocomposite materials based on gold nanoparticles for the immobilization of antibody (ab)/antigen/antibody-hrp (horseradish peroxidase) sandwich on the glassy carbon (gc) electrode surface. the electrochemical transduction was mediated by thionin, which was used in its monomeric form d ... | 2011 | 20947323 |
| simultaneous detection of multiple chemical residues in milk using broad-specificity antibodies in a hybrid immunosorbent assay. | in this study, a novel immunoassay using 2 types of sensors (qds and an enzyme) were simultaneously used for detecting multiple structurally different molecules in milk. the method integrates the fluorescence-linked immunosorbent assay (flisa) using qd605 and qd655 as probes and an enzyme-linked immunosorbent assay (elisa) using horseradish peroxidase (hrp) labeled secondary antibody. the flisa was produced by anti-sulfonamide and anti-quinolone broad-specificity monoclonal antibodies (mabs) for ... | 2010 | 20947327 |
| photooxidation of cytochrome p450-bm3. | high-valent iron-oxo species are thought to be intermediates in the catalytic cycles of oxygenases and peroxidases. an attractive route to these iron-oxo intermediates involves laser flash-quench oxidation of ferric hemes, as demonstrated by our work on the ferryl (compound ii) and ferryl porphyrin radical cation (compound i) intermediates of horseradish peroxidase. extension of this work to include cytochrome p450-bm3 (cyp102a1) has required covalent attachment of a ru(ii) photosensitizer to a ... | 2010 | 20947800 |
| microfluidic immunosensor with gold nanoparticle platform for the determination of immunoglobulin g anti-echinococcus granulosus antibodies. | this article describes a microfluidic immunosensor, developed for the detection of igg antibodies specific to echinococcus granulosus in human serum samples, which represents an alternative tool that can be used for the immunodiagnosis of hydatidosis in an automated way. our device consists of a plexiglas system with a central channel and a gold electrode. for immobilization of the e. granulosus antigen, a gold electrode was modified with the incorporation of gold nanoparticles. immobilized anti ... | 2010 | 20951112 |
| ultrasensitive electrochemical strategy for nt-probnp detection with gold nanochains and horseradish peroxidase complex amplification. | a novel electrochemical immunosensor for sensitive detection of cardiac biomarker n-terminal pro-b-type natriuretic peptide (nt-probnp) is fabricated based on the nanostructural gold and carbon nanotubes composite as desirable platform for the capture antibodies immobilization and gold nanochains (auncs) and horseradish peroxidase (hrp) complex labeled secondary antibodies (auncs-hrp-ab(2)) for signal amplification. the gold nanochains were prepared by the employment of l-ascorbic acid (aa) as a ... | 2011 | 20952179 |
| a selective assay to detect chitin and biologically active nano-machineries for chitin-biosynthesis with their intrinsic chitin-synthase molecules. | a new assay system for chitin has been developed. it comprises the chitin-binding protein chbb in fusion with a his-tag as well as with a strep-tag, the latter of which was chemically coupled to horseradish peroxidase. with the resulting complex, minimal quantities of chitin are photometrically detectable. in addition, the assay allows rapid scoring of the activity of chitin-synthases. as a result, a refined procedure for the rapid purification of yeast chitosomes (nano-machineries for chitin bi ... | 2010 | 20957083 |
| biochemical filter with sigmoidal response: increasing the complexity of biomolecular logic. | the first realization of a designed, rather than natural, biochemical filter process is reported and analyzed as a promising network component for increasing the complexity of biomolecular logic systems. key challenge in biochemical logic research has been achieving scalability for complex network designs. various logic gates have been realized, but a "toolbox" of analog elements for interconnectivity and signal processing has remained elusive. filters are important as network elements that allo ... | 2010 | 20957989 |
| dna detection of chronic myelogenous leukemia by magnetic nanoparticles. | a novel tool for the detection of bcr/abl fusion gene in chronic myelogenous leukemia (cml) was developed by a magneto-polymerase chain reaction (pcr)-enzyme linked gene technique. the forward primers covalently bound to the surface of magnetic nanoparticles allowed a convenient separation of pcr products with high sensitivity (0.5 pg ml(-1)) and high specificity using k562 cell line and cml patients. the results were obtained when the biotinylated-reverse primer bound to streptavidin-horseradis ... | 2011 | 20963240 |
| direct projections of omnipause neurons to reticulospinal neurons: a double-labeling light microscopic study in the cat. | omnipause neurons (opns) are inhibitory neurons located in the midline region of the caudal pons. their role in gating the discharges of saccade-related burst neurons is well known, but there is no agreement concerning their influence on brainstem neurons that control other muscle groups participating in rapid gaze shifts. in the present study, we inquired whether opns project directly to pontobulbar reticulospinal neurons (rsns) in the cat. retrograde transport of horseradish peroxidase from th ... | 2010 | 20963829 |
| ultrasensitive electrochemical aptasensor for thrombin based on the amplification of aptamer-aunps-hrp conjugates. | successful development of an ultrasensitive and highly specific electrochemical aptasensor for thrombin based on amplification of aptamer-gold nanoparticles-horseradish peroxidase (aptamer-aunps-hrp) conjugates was reported. in this electrochemical protocol, aptamer1 (apt1) was immobilized on core/shell fe(3)o(4)/au magnetic nanoparticles (aumnps) and served as capture probe. aptamer2 (apt2) was dual labeled with aunps and hrp and used as detection probe. in the presence of thrombin, the sandwic ... | 2010 | 21030239 |
| nutritive evaluation and effect of moringa oleifera pod on clastogenic potential in the mouse. | moringa oleifera lam (horseradish tree; tender pod or fruits) has been consumed as a vegetable and utilized as a major ingredient of healthy thai cuisine. previous studies have shown that m. oleifera pod extracts act as bifunctional inducers along with displaying antioxidant properties and also inhibiting skin papillomagenesis in mice. this study was aimed to determine the nutritive value, and clastogenic and anticlastogenic potentials of m. oleifera pod. the nutritive value was determined accor ... | 2010 | 21039028 |
| peripheral nerve injury and trpv1-expressing primary afferent c-fibers cause opening of the blood-brain barrier. | the blood-brain barrier (bbb) plays the crucial role of limiting exposure of the central nervous system (cns) to damaging molecules and cells. dysfunction of the bbb is critical in a broad range of cns disorders including neurodegeneration, inflammatory or traumatic injury to the cns, and stroke. in peripheral tissues, the vascular-tissue permeability is normally greater than bbb permeability, but vascular leakage can be induced by efferent discharge activity in primary sensory neurons leading t ... | 2010 | 21044346 |
| continuous enzymatic treatment of 4-bromophenol initiated by uv irradiation. | horseradish peroxidase (hrp) can be used for the treatment of halogenated phenolic substances. in the presence of hydrogen peroxide phenols are oxidized to form polymers which undergo partial dehalogenation. however, when immobilized, the peroxidase is subject to inactivation due to blockage of the active sites by the growing polymers and to deactivation by elevated levels of hydrogen peroxide. when hrp immobilized on a novel glass-based support incorporating titanium dioxide is subjected to uv ... | 2010 | 21045326 |
| computer-controlled system for the study of oxidase reactions: application to the peroxidase-oxidase oscillator. | an apparatus for the study of bisubstrate oxidase reactions at maintained steady-state substrate concentrations is described, and its specific application to the peroxidase-oxidase biochemical oscillator is reported. instrument control and data acquisition are provided by custom software written in labview. the software allows measurement, recording, and control of dissolved oxygen through a clark-type oxygen electrode, reaction monitoring by a uv/vis spectrophotometer, and controlled substrate ... | 2010 | 21049952 |
| prognostic significance of cyclin a and b1 in pediatric embryonal tumors. | embryonal tumors constitute the most common malignant brain tumor group in children. although patient prognosis has been substantially improved over recent decades, identification of prognostic markers would be of obvious significance. in the present study we evaluated the prognostic significance of cyclin a and b1 in correlation with ki-67 index in pediatric embryonal tumors. we retrospectively evaluated 53 children with embryonic tumors who were treated surgically in our institute. all patient ... | 2010 | 21069428 |
| randomised clinical trial: the effects of perioperative probiotic treatment on barrier function and post-operative infectious complications in colorectal cancer surgery - a double-blind study. | infection following abdominal operation remains a major factor affecting the morbidity of patients after surgery. | 2011 | 21083585 |
| increasing of blood-tumor barrier permeability through paracellular pathway by low-frequency ultrasound irradiation in vitro. | the research was conducted to study the increase of blood-tumor barrier (btb) permeability through paracellular pathway by low-frequency ultrasound (lfu) irradiation in vitro. lfu (frequency=1.0 mhz) was performed to irradiate btb model from the co-culture of rat c6 glioma cells and rat brain microvascular endothelial cells (rbmecs). the permeability of btb was measured by transendothelial electrical resistance (teer) and flux of horseradish peroxidase (hrp) assays after lfu irradiation. western ... | 2010 | 21104456 |
| stopped-flow microarray immunoassay for detection of viable e. coli by use of chemiluminescence flow-through microarrays. | rapid multiplexed analysis of microorganisms is important in water analysis to control bacterial contamination for health and safety reasons. direct quantification of bacteria by means of flow-through microarray immunoassays requires new analysis strategies for optimising sensitivity and the analysis time. for bacteria and for particles, hydrodynamic forces and sedimentation are the dominating effects for binding on surfaces in a flow-through system, whereas diffusion is insignificant. therefore ... | 2010 | 21107820 |
| a novel electrochemical immunoassay based on diazotization-coupled functionalized bioconjugates as trace labels for ultrasensitive detection of carcinoembryonic antigen. | in this article, a novel sandwich-type electrochemical immunosensor based on the signal amplification strategy of diazotization-coupling concept for ultrasensitive detection of carcinoembryonic antigen (cea) was reported. it operates through physisorption of monoclonal anti-cea on 4-aminothiophenol (4atp) functionalized gold electrode interface as the detection platform. diazo-4atp-coupled-thionine (thi)-conjugated gold nanoparticles (gnps) were prepared for immobilization of horseradish peroxid ... | 2011 | 21111600 |
| kinetic analysis of phagosomal production of reactive oxygen species. | phagocytes produce large quantities of reactive oxygen species for pathogen killing; however, the kinetics and amplitude of ros production on the level of individual phagosomes are poorly understood. this is mainly due to the lack of appropriate methods for quantitative ros detection with microscopic resolution. we covalently attached the ros-sensitive dye dichlorodihydrofluorescein (dcfh(2)) to yeast particles and investigated their fluorescence due to oxidation in vitro and in live phagocytes. ... | 2010 | 21111807 |
| enzyme linked immunosorbent assay for milk progesterone. | a direct antigen heterologous enzyme linked immunosorbent assay (elisa) for milk progesterone has been developed using progesterone-3-o-carboxymethyloxime-bovine serum albumin (p-3-o-cmo-bsa) antiserum and 17-α-hydroxy-progesterone-3-o-carboxymethyloxime-horseradish peroxidase (17-α-oh-p-3-o-cmo-hrp) enzyme conjugate. the data of the present study reveal that the homologous assay, which employed p-3-o-cmo-hrp as the label, showed no displacement. on the contrary, replacement of p-3-o-cmo-hrp wit ... | 2010 | 21113843 |
| uvb-activated indole-3-acetic acid induces apoptosis of pc-3 prostate cancer cells. | indole-3-acetic acid (iaa) has recently shown anticancer activity in combination with horseradish peroxidase. the current study demonstrated that iaa irradiated with ultraviolet b (iaa(uvb)) is able to generate free radicals and induce cell death in a time-dependent fashion in pc-3 prostate cancer cells, while pc-3 cells treated with iaa alone exhibited no toxic responses. it was also found through western blot analysis that the cytotoxic effect of iaa(uvb) resulted from apoptosis. treatment wit ... | 2010 | 21115913 |
| oxidative biodegradation of single- and multi-walled carbon nanotubes. | in this study we compare the biodegradation of both single-walled (swcnts) and multi-walled carbon nanotubes (mwcnts) using two different oxidative conditions. in particular, we demonstrate that oxidized multi-walled carbon nanotubes are highly degraded, although not to completeness when treated with horseradish peroxidase (hrp) in the presence of hydrogen peroxide. | 2011 | 21116547 |
| cation exchange in znse nanocrystals for signal amplification in bioassays. | znse nanocrystals (ncs), possessing low native luminescence but high biocompatibility, were employed as labeling tags in bioassays. they were able to amplify each target recognition event thousands of times through a cation-exchange reaction (cxamp) that released over 3000 encapsulated zn(2+) from one single nc. the freed cations in turn triggered strong fluorescence from the zn-responsive dyes. the present study demonstrated that cxamp with znse delivered superior detection performance in compa ... | 2010 | 21117624 |
| enhanced stability and decolorization of coomassie brilliant blue r-250 by dextran aldehyde-modified horseradish peroxidase. | horseradish peroxidase (ec 1.11.1.7) was chemically modified by periodate-activated dextran. the activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. modified hrp remained fully active in the presence of organic solvent for 4 h. however, the unmodified enzyme lost 50% of its activity within the first 2 h. the effects of possible inhibitors on enzyme activity were investigated. in addition, coomassie brilliant blue r-250 was efficiently deco ... | 2011 | 21117874 |
| quantitative detection of human tumor necrosis factor α by a resonance raman enzyme-linked immunosorbent assay. | tumor necrosis factor α is an inflammatory cytokine which has been linked with many infectious and inflammatory diseases. detection and quantification of this key biomarker is commonly achieved by use of an enzyme-linked immunosorbent assay (elisa). this fundamental technique uses the spectroscopic detection of a chromogen such as 3,3',5,5'-tetramethylbenzidine (tmb). horseradish peroxidase (hrp), bound to the detection antibody, catalyzes the oxidation of tmb by hydrogen peroxide to generate co ... | 2010 | 21121688 |
| photoresponsive dna-cross-linked hydrogels for controllable release and cancer therapy. | we have developed a photoresponsive dna-cross-linked hydrogel that can be photoregulated by two wavelengths with a reversible sol-gel conversion. this photoinduced conversion can be further utilized for precisely controllable encapsulation and release of multiple loads. specifically, photosensitive azobenzene moieties are incorporated into dna strands as cross-linkers, such that their hybridization to complementary dnas (cdnas) responds differently to different wavelengths of light. on the basis ... | 2011 | 21126095 |
| amperometric biosensors based on alumina nanoparticles-chitosan-horseradish peroxidase nanobiocomposites for the determination of phenolic compounds. | a horseradish peroxidase (hrp) biosensor based on alumina (al(2)o(3)) nanoparticles-chitosan (chit) nanocomposites was developed for the detection of phenolic compounds. uv-vis spectra and fourier transform infrared spectra showed that hrp retained its original structure on the al(2)o(3)/chit film. the surface morphologies of the composite films were characterized by scanning electron microscopy. cyclic voltammetry and amperometry were used to study the proposed electrochemical biosensor. optimi ... | 2011 | 21127796 |
| a novel and efficient oxidative functionalization of lignin by layer-by-layer immobilised horseradish peroxidase. | horseradish peroxidase (hrp) was chemically immobilised onto alumina particles and coated by polyelectrolytes layers, using the layer-by-layer technique. the reactivity of the immobilised enzyme was studied in the oxidative functionalisation of softwood milled wood and residual kraft lignins and found higher than the free enzyme. in order to investigate the chemical modifications in the lignin structure, quantitative (31)p nmr was used. the immobilised hrp showed a higher reactivity with respect ... | 2011 | 21130661 |