Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| development of a sensitivity-improved immunoassay for the determination of carbaryl in food samples. | with the aim of developing a highly sensitive immunoassay for carbaryl, a hapten which had high similarity to carbaryl was synthesised using a safer and more practical approach. after it was conjugated to horseradish peroxidase, direct competitive heterologous enzyme-linked immunosorbent assay (cd-elisa) was optimised and characterised. the assay performance conditions were investigated in details. enhanced chemiluminescence elisa (ecl-elisa) was also used in preliminary studies. | 2010 | 20393990 |
| morphological analysis of newly formed vessels and cellular microenvironment. | newly formed microvessels were studied by the topical method (combination of light and electron microscopy with preliminary visualization of the isolated new vessel with horseradish peroxidase) on day 8 of growth. five different zones with different transport characteristics of the vascular wall and cellular microenvironment were detected over the vascular length. a direct correlation between cellular microenvironment and formation of transporting canals in the endothelial lining of the newly fo ... | 2009 | 20396806 |
| experimental study on acupuncture activating the gonadotropin-releasing hormone neurons in hypothalamus. | to probe into the most effective site, extra-ordinary point, acupoint and channel for regulating reproductive endocrine function by means of the study on acupuncture activating the gonadotropin-releasing hormone neurons (gnrh) in hypothalamus. | 2010 | 20397460 |
| inhibitory effect of gallic acid and its esters on 2,2'-azobis(2-amidinopropane)hydrochloride (aaph)-induced hemolysis and depletion of intracellular glutathione in erythrocytes. | the protective effect of gallic acid and its esters, methyl, propyl, and lauryl gallate, against 2,2'-azobis(2-amidinopropane)hydrochloride (aaph)-induced hemolysis and depletion of intracellular glutathione (gsh) in erythrocytes was studied. the inhibition of hemolysis was dose-dependent, and the esters were significantly more effective than gallic acid. gallic acid and its esters were compared with regard to their reactivity to free radicals, using the dpph and aaph/pyranine free-cell assays, ... | 2010 | 20397726 |
| development of a blocking-elisa for the detection of antibodies against histomonas meleagridis in chickens and turkeys. | histomonosis is an infectious disease of mainly galliform birds that can cause high mortality, especially in commercial turkey flocks. several diagnostic tools were available to detect its causative agent, the flagellated protozoan histomonas meleagridis. however, serological tools were largely lacking. recently, an indirect elisa has been described, but this test was not validated for specificity and might suffer from cross-reactivity with related protozoa. therefore, a specific blocking-elisa ... | 2010 | 20400229 |
| in situ simultaneous protein-polysaccharide bioconjugation and hydrogelation using horseradish peroxidase. | we propose the peroxidase-catalyzed simultaneous conjugation and hydrogelation of polysaccharide and protein derivatives, each possessing phenolic hydroxyl (ph) moieties, as a novel route for obtaining protein-polysaccharide conjugate hydrogels. we used alginate, gelatin, and albumin derivatives bearing ph moieties (alg-ph, gela-ph, and alb-ph) to demonstrate the feasibility. the gelation time of conjugate gels decreased with decreasing h(2)o(2) concentration and with increasing horseradish pero ... | 2010 | 20402496 |
| enzyme immobilized magnetic nanoparticles for in-line capillary electrophoresis and drug biotransformation studies: application to paracetamol. | enzyme immobilized magnetic nanoparticles (emnps) were injected and magnetically retained, as a microreactor, in the capillary of a capillary electrophoresis (ce) setup with uv detection. the enzyme horseradish peroxidase (hrp) was chemically immobilized onto commercially available magnetic 300 nm diameter nanoparticles. paracetamol (acetaminophen: n-acetyl-p-aminophenol), a common analgesic drug, was used as model drug compound. the enzymatic reaction was studied in-line by ce in 12.5 mm phosph ... | 2010 | 20402645 |
| meal parameters and vagal gastrointestinal afferents in mice that experienced early postnatal overnutrition. | early postnatal overnutrition results in a predisposition to develop obesity due in part to hypothalamic and sympathetic dysfunction. potential involvement of another major regulatory system component--the vagus nerve--has not been examined. moreover, feeding disturbances have rarely been investigated prior to development of obesity when confounds due to obesity are minimized. to examine these issues, litters were culled on the day of birth to create small litters (sl; overnutrition), or normal ... | 2010 | 20403369 |
| exploring peptide space for enzyme modulators. | a method is presented for screening high-density arrays to discover peptides that bind and modulate enzyme activity. a polyvinyl alcohol solution was applied to array surfaces to limit the diffusion of product molecules released from enzymatic reactions, allowing the simultaneous measurement of enzyme activity and binding at each peptide spot. for proof of concept, it was possible to identify peptides that bound to horseradish peroxidase, alkaline phosphatase, and beta-galactosidase and substant ... | 2010 | 20408521 |
| toxic effect of heavy metal terbium ion on cell membrane in horseradish. | in order to understand the toxic mechanism of terbium ion (tb(iii)) on plants, the subcellular distribution of tb(iii) in horseradish, the effect of tb(iii) on the composition of the fatty acids in the cell membrane, the peroxidation of membrane lipid, the morphological character of protoplast, the cellular ultrastructure in horseradish were investigated using transmission electron microscopic autoradiography, molecular dynamics simulation, gas chromatography, scanning electron microscopy and tr ... | 2010 | 20413142 |
| expression of astrocytic type 2 angiotensin receptor in central nervous system inflammation correlates with blood-brain barrier breakdown. | the blood-brain barrier (bbb), a complex of endothelial and glial barriers, controls passage of cells and solutes between the blood and central nervous system (cns). blood-brain barrier breakdown refers to entry of cells and/or solutes. we were interested whether the renin-angiotensin system is involved during bbb breakdown. we studied the type 2 angiotensin receptor at(2) because of its suggested neuroprotective role. two models of brain inflammation were used to distinguish solute versus cellu ... | 2010 | 20414743 |
| effects of heavy metal terbium on contents of cytosolic nutrient elements in horseradish cell. | the wide application of rare earth elements (rees) has led to the accumulation of rees in soil and plant. thereby, the effect of tb(3+) on the contents of cytosolic nutrient elements in horseradish was investigated with the synchronous detection technique of scanning electron microscope and energy dispersive x-ray spectrometry. it was found for the first time that the foliar spraying treatment of tb(3+) destroyed the structure of horseradish mesophyll cells, and then changed the contents of the ... | 2010 | 20417560 |
| an excellent enzyme biosensor based on sb-doped sno2 nanowires. | sb-doped sno(2) nanowires were synthesized via thermal evaporation. scanning electron microscopic, transmission electron microscopic, x-ray diffraction, current-voltage, and electrochemical impedance spectroscopy experiments have been used to characterize the structural and electrical behaviors of the nanowires. a mediator-free horseradish peroxidase-based h(2)o(2) biosensor was constructed through the sb-doped sno(2) nanowires used as the immobilization matrix for the enzymes. in comparison wit ... | 2010 | 20418091 |
| development and evaluation of a sensitive pcr-elisa system for detection of schistosoma infection in feces. | a pcr-enzyme-linked immunosorbent assay (pcr-elisa) was developed to overcome the need for sensitive techniques for the efficient diagnosis of schistosoma infection in endemic settings with low parasitic burden. | 2010 | 20421918 |
| detection of biomarkers with carbon nanotube-based immunosensors. | a facile and capable method of preparation of sensitive carbon nanotube (cnt)/polysulfone/rigg immunosensor is discussed in this chapter. the immunosensor is based on the modification of disposable screen-printed electrodes by phase inversion method. cnt/polysulfone membrane acts as the reservoir of immunomolecules as well as a transducer. this configuration offers large surface area, elevated porosity, and mechanical flexibility. the comparison with graphite/polysulfone/rigg immunosensors shows ... | 2010 | 20422394 |
| determination of optimum process parameters for peroxidase-catalysed treatment of bisphenol a and application to the removal of bisphenol derivatives. | systematic investigations were carried out to determine the optimum process parameters such as the hydrogen peroxide (h2o2) concentration, concentration and molar mass of poly(ethylene glycol) (peg) as an additive, ph value, temperature and enzyme dose for treatment of bisphenol a (bpa) with horseradish peroxidase (hrp). the hrp-catalysed treatment of bpa was effectively enhanced by adding peg, and bpa was completely converted into phenoxy radicals by hrp dose of 0.10 u/cm3. the optimum conditio ... | 2010 | 20426266 |
| polyclonal antibody against the dpv ul46m protein can be a diagnostic candidate. | the duck plague virus (dpv) ul46 protein (vp11/12) is a 739-amino acid tegument protein encoded by the ul46 gene. we analyzed the amino acid sequence of ul46 using bioinformatics tools and defined the main antigenic domains to be between nucleotides 700-2,220 in the ul46 sequence. this region was designated ul46m. the dpv ul46 and ul46m genes were both expressed in escherichia coli rosetta (de3) induced by isopropy1-beta-d-thiogalactopyranoside (iptg) following polymerase chain reaction (pcr) am ... | 2010 | 20429885 |
| direct electrochemistry and bioelectrocatalysis of horseradish peroxidase based on gold nano-seeds dotted tio2 nanocomposite. | gold nano-seeds (gnss) (capital ef, cyrillic=2-5 nm) were dotted in tio(2) colloids and the horseradish peroxidase (hrp) was successfully immobilized on the as-made gnss-tio(2) nanocomposite by a convenient and effective method. the matrix integrates the merits of both gnss and tio(2), which provides a favorable microenvironment for the immobilization of hrp. the cyclic votammetric results demonstrated that the entrapped hrp achieves direct electron transfer at glassy carbon electrode (gce). a p ... | 2010 | 20430608 |
| luminol/antibody labeled gold nanoparticles for chemiluminescence immunoassay of carcinoembryonic antigen. | a facile strategy by loading luminol and secondary antibody on gold nanoparticles (au nps) was described in the present work. the as-prepared luminol/antibody labeled au nps conjugates (laau nps) were used as the chemiluminescent probe for the detection of carcinoembryonic antigen (cea) in serum. the laau nps were characterized by transmission electron microscopy (tem), uv-vis spectrophotometry (uv-vis), and chemiluminescent method. stable and efficient chemiluminescence (cl) was obtained when l ... | 2010 | 20433970 |
| ph-sensitive water-soluble nanospheric imprinted hydrogels prepared as horseradish peroxidase mimetic enzymes. | 2010 | 20437497 | |
| computational study on compound i redox-active species in horseradish peroxydase enzyme: conformational fluctuations and solvation effects. | molecular dynamics simulations for the compound i species in horseradish peroxidase were carried out over a nanoseconds time-scale. results indicate that the supramolecular assembly composed of compound i in interaction with highly conserved distal residues (his42 and arg38) exists in two well-defined conformations basically differing in the local position of the distal histidine (i.e., his42). furthermore, we observe the presence of a biological channel in the distal side of the heme cavity, be ... | 2010 | 20438084 |
| molecular and cellular mechanism of the effect of la(iii) on horseradish peroxidase. | horseradish is an important economic crop. it contains horseradish peroxidase (hrp) and lots of nutrients, and has specific pungency. lanthanum is one of the heavy metals in the environment. it can transfer through the food chain to humans. in this paper, the molecular and cellular mechanism of the toxic effects of la(iii) on hrp in vivo was investigated with an optimized combination of biophysical, biochemical, and cytobiological methods. it was found that la(iii) could interact with o and/or n ... | 2010 | 20440526 |
| clickable gold nanoparticles as the building block of nanobioprobes. | a new method of fabricating "clickable" gold nanoparticles that could be used as the building block of nanobioprobes was described. on the basis of a well-developed strategy of encapsulating hydrophobic nanoparticles with a layer of amphiphilic polymers, cheap, easily prepared graft polymer was used as a modifier to prepare monodisperse azide-functionalized gold nanoparticles (aunps), which showed good stability in physiological solution. by conjugation with alkyne functional horseradish peroxid ... | 2010 | 20441155 |
| amplified biosensing using the horseradish peroxidase-mimicking dnazyme as an electrocatalyst. | the hemin/g-quadruplex horseradish peroxidase-mimicking dnazyme is assembled on au electrodes. it reveals bioelectrocatalytic properties and electrocatalyzes the reduction of h(2)o(2). the bioelectrocatalytic functions of the hemin/g-quadruplex dnazyme are used to develop electrochemical sensors that follow the activity of glucose oxidase and biosensors for the detection of dna or low-molecular-weight substrates (adenosine monophosphate, amp). hairpin nucleic structures that include the g-quadru ... | 2010 | 20441165 |
| novel colorimetric enzyme immunoassay for the detection of carcinoembryonic antigen. | we developed a highly sensitive colorimetric enzyme immunoassay for the detection of carcinoembryonic antigen (cea). this method employed gold nanoparticles (aunps) as carriers of anti-cea antibody labeled with biotin, which served as an affinity tag for streptavidin-horseradish peroxidase (streptavidin-hrp) binding. using this strategy, about 12 hrp molecules were coated onto each aunp. through "sandwich-type" immunoreaction, the aunp-anti-cea-hrp complex was brought into the proximity of magne ... | 2010 | 20441949 |
| simultaneous determination of biomarkers for alzheimer's disease using sol-gel-derived optical array biosensor. | the sol-gel-derived optical array-based biosensor for the simultaneous analysis of multiple analytes as potential markers for alzheimer's disease was fabricated. beta-amyloid, acetylcholine and glutamate were selected as the biosensing probes. the fluorescent dye, carboxy snarf-1-dextran, was co-immobilized with glutamate dehydrogenase and acetylcholinesterase for sensing glutamate and acetylcholine, respectively, while amplex red and fitc-dextran were immobilized with horseradish peroxidase for ... | 2010 | 20444591 |
| multifunctional magnetic bead-based electrochemical immunoassay for the detection of aflatoxin b1 in food. | a sensitive and reusable electrochemical immunoassay for aflatoxin b(1) (afb(1)) in food has been developed. a multifunctional magnetic bead (mmb) was initially synthesized using magnetic cofe(2)o(4) nanoparticle as the core and prussian blue nanoparticle (pbnp)-doped silica as the shell, and then the prepared mmb was used as an affinity support for the immobilization of the afb(1)-bovine serum albumin conjugate (afb(1)-bsa). with the aid of an external magnet, the afb(1)-bsa-conjugated mmbs wer ... | 2009 | 20448920 |
| fabrication of an amperometric bienzyme biosensing system with neutral red functionalized carbon nanotubes. | novel nanomaterials for biosensor applications represent a rapidly progressing field of nanotechnology. an exploration of functionalized multi-walled carbon nanotubes (mwnts) as a platform for amperometric determination of glucose employing the enzymes glucose oxidase (gox) and horseradish peroxidase (hrp), which were immobilized with neutral red (nr) functionalized mwnts is presented. the fabrication and analytical characterization of the glucose biosensor for the measurement of low concentrati ... | 2009 | 20448929 |
| enhanced chemiluminescence: a sensitive analytical system for detection of sweet potato peroxidase. | 3-(10'-phenothiazinyl)propane-1-sulfonate (sptz) was shown to be a potent enhancer of anionic sweet potato peroxidase (aspp)-induced chemiluminescence. the optimal conditions for aspp-catalyzed oxidation of luminol were investigated by varying the concentrations of luminol, hydrogen peroxide, tris, and sptz as well as the ph values of the reaction mixture. addition of 4-morpholinopyridine (morp) to the reaction mixture markedly increased the light intensity. using sptz and morp together enhanced ... | 2010 | 20449843 |
| metabolic stability of superoxide and hydroxyl radical adducts of a cyclic nitrone toward rat liver microsomes and cytosol: a stopped-flow esr spectroscopy study. | the metabolic stability of the spin adducts derived from the reaction of superoxide and hydroxyl radicals with 5-tert-butoxycarbonyl-5-methyl-1-pyrroline n-oxide (bocmpo) in the presence of rat liver microsomes (rlm) and rat liver cytosol (rlc) was studied by using a stopped-flow device coupled to an electron spin resonance (esr) spectrometer. the kinetics of the disappearance of the bocmpo-oh and bocmpo-ooh radicals could be followed by esr spectroscopy with treatment of the esr data by an appr ... | 2010 | 20452418 |
| protection against early intestinal compromise by lipid-rich enteral nutrition through cholecystokinin receptors. | early gut wall integrity loss and local intestinal inflammation are associated with the development of inflammatory complications in surgical and trauma patients. prevention of these intestinal events is a potential target for therapies aimed to control systemic inflammation. previously, we demonstrated in a rodent shock model that lipid-rich enteral nutrition attenuated systemic inflammation and prevented organ damage through a cholecystokinin receptor-dependent vagal pathway. the influence of ... | 2010 | 20453642 |
| structures of two novel trimeric stilbenes obtained by horseradish peroxidase catalyzed biotransformation of trans-resveratrol and (-)-epsilon-viniferin. | two stilbenes, trans-resveratrol and (-)-epsilon-viniferin, as well as a mixture of both, were biotransformed using horseradish peroxidase and hydrogen peroxide. under the applied conditions trans-resveratrol afforded one major product, which was identified as trans-delta-viniferin, a resveratrol-trans-dehydrodimer. large-scale biotransformation of a mixture of trans-resveratrol and (-)-epsilon-viniferin yielded two novel resveratrol trimers, resviniferin a and resviniferin b, which were obtaine ... | 2010 | 20455561 |
| delivering horseradish peroxidase as a respirable powder to the isolated, perfused, and ventilated lung of the rat: the pulmonary disposition of an inhaled model biopharmaceutical. | our aim was to investigate the potential of the dustgun aerosol technology integrated with the isolated, perfused, and ventilated lung of the rat (ipl) to study the pulmonary disposition of an inhaled model biopharmaceutical, the 40-kda protein horseradish peroxidase (hrp). | 2010 | 20455769 |
| brain distribution and toxicological evaluation of a systemically delivered engineered nanoscale ceria. | engineered nanoscale ceria is used as a diesel fuel catalyst. little is known about its mammalian central nervous system effects. the objective of this paper is to characterize the biodistribution of a 5-nm citrate-stabilized ceria dispersion from blood into brain and its pro- or antioxidant effects. an approximately 4% aqueous ceria dispersion was iv infused into rats (0, 100, and up to 250 mg/kg), which were terminated after 1 or 20 h. ceria concentration, localization, and chemical speciation ... | 2010 | 20457660 |
| [segmental and regional innervation of acupoint "taixi" (ki 3) area in the rat: a horseradish peroxidase method study]. | to study the peripheral and central neural involvements of acupoint "taixi" (ki 3) by using horseradish peroxidase (hrp). | 2010 | 20458903 |
| ultrasensitive optical detection of hydrogen peroxide by triggered activation of horseradish peroxidase. | hydrogen peroxide is a very reactive byproduct of many metabolic pathways. we describe an ultra-sensitive colorimetric method to detect hydrogen peroxide based on the reconstitution of apo-horseradish peroxidase with the hemin derivative, hemin di(n,n'-acetyl-hydrazide). oxidation of the latter by hydrogen peroxide yields hemin, which is able to reconstitute apo-horseradish peroxidase, forming an active peroxidase. we have also applied this analyte-triggered reconstitution of horseradish peroxid ... | 2010 | 20464020 |
| development and evaluation of a rotary cell for capillary electrophoresis-chemiluminescence detection. | many efforts have been made toward the advancement of capillary electrophoresis chemiluminescence (ce-cl) detection and its applications through continuous development and improvement of interfaces. in this study, a novel rotary cell for ce-cl detection was fabricated and evaluated. a ring-shaped narrow channel with a quartz bottom is made in a cell body to hold cl reactants and act as the reaction chamber. the ce capillary is placed closely to the bottom of the reaction chamber where analyte is ... | 2010 | 20469921 |
| amyloid hydrogel derived from curly protein fibrils of alpha-synuclein. | elucidation of molecular assembly mechanism of protein-based suprastructure formation is pivotal to develop biomaterials. a single amyloidogenic protein of alpha-synuclein turned into two morphologically distinctive amyloid fibrils - 'curly' (caf) vs. 'straight' (saf) - depending on its fibrillation processes. mutually exclusive production of caf and saf was achieved with either centrifugal membrane filtration of the preformed oligomeric species of alpha-synuclein or agitated incubation of its m ... | 2010 | 20471079 |
| combined use of fluorescent dyes and flow cytometry to quantify the physiological state of pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures. | matching both the construction of a recombinant strain and the process design with the characteristics of the target protein has the potential to significantly enhance bioprocess performance, robustness, and reproducibility. the factors affecting the physiological state of recombinant pichia pastoris mut(+) (methanol utilization-positive) strains and their cell membranes were quantified at the individual cell level using a combination of staining with fluorescent dyes and flow cytometric enumera ... | 2010 | 20472737 |
| conductive carbon nanoparticles-based electrochemical immunosensor with enhanced sensitivity for alpha-fetoprotein using irregular-shaped gold nanoparticles-labeled enzyme-linked antibodies as signal improvement. | a new electrochemical immunoassay protocol for sensitive detection of alpha-fetoprotein (afp, as a model) is designed using carbon nanoparticles (cnps)-functionalized biomimetic interface as immunosensing probe and irregular-shaped gold nanoparticles (isngs)-labeled horseradish peroxidase-anti-afp conjugates (hrp-anti-afp-isng) as trace label. the low-toxic and high-conductive cnps provided a high capacity nanoparticulate immobilization surface and a facile pathway for electron transfer. in comp ... | 2010 | 20483583 |
| coupled enzyme reactions in multicompartment microparticles. | spherical biopolymer particles have been fabricated, applying coprecipitation with calcium carbonate, followed by cross-linking of the macromolecules and dissolution of the inorganic support. particles made of roughly 80% horseradish peroxidase (hrp) as well as glucose oxidase (gox) were prepared and enzyme activities were confirmed, applying the amplex red assay. the enzyme particles were reusable for at least six times, with a remaining activity of 30-50% from the initial one. when multiple co ... | 2010 | 20486658 |
| vinyl sulfone bifunctional tag reagents for single-point modification of proteins. | the introduction of multiple labels onto biomolecules is a challenge. we report herein the synthesis of vinyl sulfone derivatized bifunctional tag single-attachment-point reagents (btsap) bearing biotin and a fluorescent tag and their applications in proteins for the introduction of multiple labels by means of the michael-type addition of the electrophilic vinyl sulfone group. these btsap reagents were easily synthesized by a two-step chemical strategy involving the preparation of alkyne vinyl s ... | 2010 | 20496947 |
| damage to the high-affinity ?-aminobutyric acid (gaba) uptake system in mouse brain by horseradish peroxidase (hrp). | presynaptic nerve terminals when depolarized are sensitive to morphological and functional alteration by horseradish peroxidase. mouse brain slices, 0.1 mm, depolarized by a k(+)-hepes buffer and exposed to horseradish peroxidase exhibited alterations in both synaptic vesicle membrane structure and in high-affinity [(14)c]?-aminobutyric acid uptake. the post stimulatory retrieval of synaptic vesicles from the nerve terminal plasma membrane in the presence of horseradish peroxidase resulted in a ... | 1987 | 20501064 |
| protein radical formation resulting from eosinophil peroxidase-catalyzed oxidation of sulfite. | eosinophil peroxidase (epo) is an abundant heme protein in eosinophils that catalyzes the formation of cytotoxic oxidants implicated in asthma, allergic inflammatory disorders, and cancer. it is known that some proteins with peroxidase activity (horseradish peroxidase and prostaglandin hydroperoxidase) can catalyze oxidation of bisulfite (hydrated sulfur dioxide), leading to the formation of sulfur trioxide anion radical ((.)so(3)(-)). this free radical further reacts with oxygen to form peroxym ... | 2010 | 20501663 |
| a rapid enzyme immunoassay for cholectystokinin octapeptide sulfate. | a rapid and sensitive enzyme immunoassay (eia) for cholecystokinin octapeptide sulfate (cck-8s) has been developed. the assay is based on a double antibody method using n-terminal specific antibody to cck-8s, and cck-8s conjugated with horseradish peroxidase as the enzyme labeled antigen. the time for the first incubation was 2 h. the time for the second incubation, during which the first antibody was precipitated using the second antibody which contained polyethylene glycol 6000 as an accelerat ... | 1989 | 20504465 |
| colorimetric quantification of mrna expression in rare tumour cells amplified by multiple ligation-dependent probe amplification. | an enzyme-linked oligonucleotide assay (elona) for quantification of mrna expression of five genes involved in breast cancer, extracted from isolated rare tumour cells and amplified by multiplex ligation-dependent probe amplification (mlpa) is presented. in mlpa, a multiplex oligonucleotide ligation assay is combined with a pcr reaction in which all ligation products are amplified by use of a single primer pair. biotinylated probes complementary to each of the target sequences were immobilised o ... | 2010 | 20526769 |
| methods for detection and measurement of hydrogen peroxide inside and outside of cells. | hydrogen peroxide (h(2)o(2)) is an incompletely reduced metabolite of oxygen that has a diverse array of physiological and pathological effects within living cells depending on the extent, timing, and location of its production. characterization of the cellular functions of h(2)o(2) requires measurement of its concentration selectively in the presence of other oxygen metabolites and with spatial and temporal fidelity in live cells. for the measurement of h(2)o(2) in biological fluids, several se ... | 2010 | 20526816 |
| enzyme biosensor based on the immobilization of hrp on sio₂/bsa/au composite nanoparticles. | in this work, an enzyme biosensor based on the immobilization of horseradish peroxidase (hrp) on sio₂/bsa/au/thionine/nafion-modified gold electrode was fabricated successfully. firstly, nafion was dropped on the surface of the gold electrode to form a nafion film followed by chemisorption of thionine (thi) as an electron mediator via the ion-exchange interaction between the thi and nafion. subsequently, the sio₂/bsa/au composite nanoparticles were assembled onto thi film through the covalent bo ... | 2010 | 20532672 |
| connections between the zona incerta of the dog diencephalon and the substantia nigra, ventral tegmental field, and pedunculopontine tegmental nucleus. | studies using technique based on retrograde and anterograde transport of horseradish peroxidase were performed to address the organization of the connections of different sectors of the zone incerta (zi) of the diencephalon with substructures of the substantia nigra, ventral tegmental field, and pedunculopontine nucleus of the midbrain in dogs. these structures were found to be interconnected. the organization of these connections included elements demonstrating that segregated conduction of fun ... | 2010 | 20532829 |
| [ontogenetic specifics of organisation of the cortico-cortical connections of the cat primary visual cortex and lateral suprasylvian area]. | a quantitative and qualitative analysis of distribution of initial cells that organize the cortico-cortical connections between area 17 and posterior-medial lateral suprasylvian area (pmls) was carried out in 10 kittens (5- and 12-week old). a horseradish peroxidase labeling technique was used. pmls area is the highest center of moving visual stimuli information processing. certain ontogenetic peculiarities of the area 17 initial neurons regular (clustered) organization were revealed. an age-rel ... | 2010 | 20535992 |
| electrochemical immunosensors for antibodies to peanut allergen ara h2 using gold nanoparticle-peptide films. | life-threatening allergies to peanuts and tree nuts can be revealed by detecting antibodies (iges) to their allergens in patient serum. herein, we compare several immunosensor-like methodologies for sensitive detection of antibodies to a peptide sequence from the major peanut allergen, arachis hypogaea 2 (ara h2). the sensors feature a synthetic peptide layer of the major ige-binding epitope from ara h2 attached to a dense gold nanoparticle (aunp) film on a pyrolytic graphite (pg) electrode. the ... | 2010 | 20540504 |
| [immortalized mouse brain endothelial cell line bend.3 displays the comparative barrier characteristics as the primary brain microvascular endothelial cells]. | the purpose of this study was to assess weather the immortalized mouse brain endothelial cell line bend.3 displays the comparative barrier characteristics as the primary brain microvascular endothelial cells (bemc). | 2010 | 20540860 |
| nano-molar level hydrogen peroxide detection by horseradish peroxidase adsorbed cup-stacked carbon nanotube electrodes and applications to l-glutamate detection. | we have developed a simple fabrication method of a highly sensitive direct electron transfer-type electrochemical biosensor for hydrogen peroxide by use of cup-stacked carbon nanotubes (cscnts). the cscnts, formed by stacking of cup-shaped carbon units, has larger internal space and more hydrophilic edges, thanks to the presence of functional groups containing oxygen (e.g., -cooh, -oh), than multi-walled carbon nanotubes (mwcnts). when the cscnts suspension was cast, the cscnts were dispersed ho ... | 2010 | 20543499 |
| oral bifidobacterium modulates intestinal immune inflammation in mice with food allergy. | it is proposed that probiotics have a therapeutic effect on the treatment of immune disorders. however, the underlying mechanisms require clarification. the present study aimed to evaluate the effect of gavage-feeding bifidobacteria on suppression of t helper 2 (th2) pattern inflammation in the intestines of mice with food allergy. | 2010 | 20546446 |
| protein modification with amphiphilic block copoly(2-oxazoline)s as a new platform for enhanced cellular delivery. | several homopolymers, random copolymers and block copolymers based on poly(2-oxazoline)s (pox) were synthesized and conjugated to horseradish peroxidase (hrp) using biodegradable and nonbiodegradable linkers. these conjugates were characterized by amino group titration, polyacrylamide gel electrophoresis (page), isoelectric focusing, enzymatic activity assay and conformation analysis. the conjugates contained on average from about one to two polymer chains per enzyme. from 70% to 90% of enzymati ... | 2010 | 20550191 |
| activity-dependent bulk endocytosis and clathrin-dependent endocytosis replenish specific synaptic vesicle pools in central nerve terminals. | multiple synaptic vesicle (sv) retrieval modes exist in central nerve terminals to maintain a continual supply of svs for neurotransmission. two such modes are clathrin-mediated endocytosis (cme), which is dominant during mild neuronal activity, and activity-dependent bulk endocytosis (adbe), which is dominant during intense neuronal activity. however, little is known about how activation of these sv retrieval modes impact the replenishment of the total sv recycling pool and the pools that resid ... | 2010 | 20554865 |
| [combination of matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry for the analysis of intact glycopeptides from horseradish peroxidase]. | the mass spectrometric analysis of glycan composition and structure is a difficult but essential part in future study following the present glycoprotein identification. intact glycopeptides analysis is an attracting field, in considering its capability to provide glycosite and corresponding glycan structure information at the same time. mass spectrometry has been proven to be an important and a key tool for glycan analysis over the past few years. making use of two soft ionization techniques-mat ... | 2010 | 20556950 |
| proteolysis of submembrane cytoskeletal proteins ankyrin-g and αii-spectrin following diffuse brain injury: a role in white matter vulnerability at nodes of ranvier. | a high membrane-to-cytoplasm ratio makes axons particularly vulnerable to traumatic injury. posttraumatic shifts in ionic homeostasis promote spectrin cleavage, disrupt ankyrin linkages and destabilize axolemmal proteins. this study contrasted ankyrin-g and αii-spectrin degradation in cortex and corpus callosum following diffuse axonal injury produced by fluid percussion insult. ankyrin-g lysis occurred preferentially in white matter, with acute elevation of all fragments and long-term reduction ... | 2010 | 20557305 |
| prognostic value of mucin 4 expression in colorectal adenocarcinomas. | mucin 4 (muc4) is aberrantly expressed in colorectal adenocarcinomas (crcs) but its prognostic value is unknown. | 2010 | 20564074 |
| chemiluminescently labeled aptamers as the affinity probe for interaction analysis by capillary electrophoresis. | aptamers are nucleic acid oligonucleotides, which can recognize targets with high affinity and specificity. fluorescently labeled aptamers have been used as affinity probes in ce for interaction analysis. in this study, a method of labeling aptamers chemiluminescently with isoluminol isothiocyanate (ilitc) through covalent bonds was proposed and realized. the ilitc-labeled aptamers were characterized by hplc-ms and purified by hplc. after desalination, the ilitc-labeled aptamers were employed as ... | 2010 | 20564271 |
| time dependent differential yield as a scale-up parameter in enzyme and fermentation reactors. | the yield function, for an enzyme-substrate kinetic model of the system, is investigated. considering y to be a function of the substrate concentration s, its value as s --> o(+) is investigated.in the "pseudo-steady-state domain," this differential yield function is shown to be bounded above and below by yield functions that are obtained by using the michaelis-menten and briggs-haldane functions to relate the enzyme-substrate concentration c to s. it is also shown that the yield constant, which ... | 1986 | 20568184 |
| molecularly imprinted sensor based on an enzyme amplifier for ultratrace oxytetracycline determination. | a novel strategy for preparing highly sensitive, molecularly imprinted sensors based on enzyme amplifiers was proposed for oxytetracycline (otc) determination. a molecularly imprinted polymer (mip) film was used as an artificial antibody to interact with otc and horseradish peroxidase-labeled otc (hrp-otc). oxytetracycline was determined according to the competition reaction. the molecularly imprinted sensor was characterized by alternating current (ac) impedance spectroscopy, differential pulse ... | 2010 | 20568713 |
| adsorption and inactivation behavior of horseradish peroxidase on various substrates. | to produce bioactive papers, i.e. papers incorporating biomolecules that are useful for analyte detection, adequate immobilization strategies should be devised. in this article, the physical immobilization behavior and activity of the enzyme horseradish peroxidase (hrp) on various papermaking substrates were studied. the papermaking substrates included amorphous and crystalline cellulose, calcium carbonate, styrene butadiene latex, polystyrene, and both negatively charged rayon and rayon with a ... | 2010 | 20570116 |
| proliferative cell types in embryonic lineages of the central complex of the grasshopper schistocerca gregaria. | the central complex of the grasshopper schistocerca gregaria develops to completion during embryogenesis. a major cellular contribution to the central complex is from the w, x, y, z lineages of the pars intercerebralis, each of which comprises over 100 cells, making them by far the largest in the embryonic protocerebrum. our focus has been to find a cellular mechanism that allows such a large number of cell progeny to be generated within a restricted period of time. immunohistochemical visualiza ... | 2010 | 20571828 |
| radio frequency radiation causes no nonthermal damage in enzymes and living cells. | the ability of radio frequency radiation (rfr) to exert irreversible nonthermal (i.e., not caused by accompanying heat) effects on biologics has been widely debated due to a relative paucity of comprehensive critical details in published reports dealing with this issue. in this study, we used rigorous control over experimental conditions to determine whether continuous rfr nonthermally affects commercially important enzymes and live bacterial and human cells using three most commonly used freque ... | 2010 | 20572294 |
| [the striopallidal projections of the zona incerta in the dog diencephalon]. | the projections of individual sectors of the zona incerta (zi) in dog diencephalon to the functionally different striatal segments were studied by the method of the antero- and retrograde axonal transport of horseradish peroxidase. the data on the interconnections of zi with all striopallidal structures, obtained by the author before and in the present work were summarized. among all the striatal nuclei, the the rostral, ventral and caudal zi sectors contained the neurons that projected their fi ... | 2010 | 20572386 |
| molecular understanding of sterically controlled compound release through an engineered channel protein (fhua). | recently we reported a nanocontainer based reduction triggered release system through an engineered transmembrane channel (fhua delta1-160; onaca et al., 2008). compound fluxes within the fhua delta1-160 channel protein are controlled sterically through labeled lysine residues (label: 3-(2-pyridyldithio)propionic-acid-n-hydroxysuccinimide-ester). quantifying the sterical contribution of each labeled lysine would open up an opportunity for designing compound specific drug release systems. | 2010 | 20579361 |
| fabrication of a biocompatible and conductive platform based on a single-stranded dna/graphene nanocomposite for direct electrochemistry and electrocatalysis. | a novel electrochemical platform was designed by combining the biocompatibility of single-stranded dna (ss-dna) and the excellent conductivity of graphene (gp). this nanocomposite (denoted as ss-dna/gp) was first used as an electrode material for the immobilization and biosensing of redox enzymes. on the basis of electrostatic interactions, horseradish peroxidase (hrp) self-assembled with ss-dna/gp on the surface of a glassy carbon (gc) electrode to form an hrp/ss-dna/gp/gc electrode. uv/vis and ... | 2010 | 20583058 |
| cholecystokinin/cholecystokinin-1 receptor-mediated peripheral activation of the afferent vagus by enteral nutrients attenuates inflammation in rats. | the current study investigates activation of the nutritional anti-inflammatory pathway by lipid-rich nutrition. | 2010 | 20585240 |
| tin oxide nanorod array-based electrochemical hydrogen peroxide biosensor. | sno2 nanorod array grown directly on alloy substrate has been employed as the working electrode of h2o2 biosensor. single-crystalline sno2 nanorods provide not only low isoelectric point and enough void spaces for facile horseradish peroxidase (hrp) immobilization but also numerous conductive channels for electron transport to and from current collector; thus, leading to direct electrochemistry of hrp. the nanorod array-based biosensor demonstrates high h2o2 sensing performance in terms of excel ... | 2010 | 20596358 |
| the application of complex multiple forklike zno nanostructures to rapid and ultrahigh sensitive hydrogen peroxide biosensors. | a new complex multiple forklike zno nanostructure was fabricated by citric acid assisted annealing process. the complex multiple forklike zno nanostructure is composed of several nanorods. these nanorods grow from a thin platelet base and are parallel to each other to form complex multiple forklike zno nanostructure. the widthes of thin platelet bases range from 60 to 230 nm. the diameters of the nanorods range from 16 to 60 nm, and their lengths are 0.5 mu approximately 1.3mu. ft-ir spectrum, r ... | 2010 | 20598367 |
| nanosilver-doped dna polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies. | a simple and sensitive electrochemical immunoassay protocol was developed for the detection of carcinoembryonic antigen (cea) using nanosilver-doped dna polyion complex membrane (pic) as sensing interface. to construct such an immunosensor, double-stranded dna was initially assembled onto the surface of thionine/nafion-modified screen-printed carbon electrode to adsorb silver ions with positive charges, then silver ions were reduced to nanosilver particles with the aid of nabh(4), and then anti- ... | 2010 | 20599025 |
| biosensor for multiplex detection of two dna target sequences using enzyme-functionalized au nanoparticles as signal amplification. | multiplex electrochemical detection of two dna target sequences in one sample using enzyme-functionalized au nanoparticles (aunps) as catalytic labels for was proposed. this dna sensor was fabricated using a "sandwich" detection strategy, involving two kinds of capture probes dna immobilized on glassy carbon electrode (gce), and hybridization with target dna sequences, which further hybridized with the reporter dna loaded on the aunps. the aunp contained two kinds of dna sequences, one was compl ... | 2010 | 20599026 |
| determination of plasma and serum l-lysine using l-lysine epsilon-oxidase from marinomonas mediterranea nbrc 103028(t). | this article describes a successful application of l-lysine epsilon-oxidase (ec 1.4.3.20) for l-lysine determination. l-lysine epsilon-oxidase was isolated from culture supernatant of marinomonas mediterranea nbrc 103028(t) and was used for l-lysine determination. comparison of the characteristics of l-lysine epsilon-oxidase with l-lysine alpha-oxidase, a commercial enzyme used for l-lysine determination, suggests that the use of l-lysine epsilon-oxidase would be more valuable for the determinat ... | 2010 | 20599635 |
| a direct competitive enzyme-linked immunosorbent assay by antibody coated for diethyl phthalate analysis. | a direct competitive enzyme-linked immunosorbent assay (elisa) has been developed for detection of diethyl phthalate (dep). protein-hapten conjugate was synthesized to produce polyclonal antibodies against dep. experimental parameters were optimized, including immunoreaction conditions, the dilution ratio of horseradish peroxidase (hrp)-antigen conjugate, time of the antibody coated, effect of ph, and ionic strength. the limit of detection was 0.096ng/ml, and the linear range was 0.1-3500ng/ml w ... | 2010 | 20599639 |
| igg2 dominancy and carbohydrate recognition specificity of c3h/he mouse antibodies directed to cross-reactive carbohydrate determinants (ccds) bearing beta-(1,2)-xylose and alpha-(1,3)-fucose. | few common carbohydrate epitopes consisting of terminal beta-(1,2)-xylose and/or alpha-(1,3)-fucose residues are shared by a variety of glycoproteins from plants, insects and parasitic worms, termed cross-reactive carbohydrate determinant (ccd), and frequently recognized by ige antibodies of patients with food and/or respiratory allergy, though clinical relevancy of such ccd-specific ige is still controversial. attention has also been focused on ccds from the undesired post-translational modific ... | 2010 | 20600324 |
| antioxidant and pro-oxidant properties of acylated pelargonidin derivatives extracted from red radish (raphanus sativus var. niger, brassicaceae). | the antioxidant and pro-oxidant potential of an extract from red radish, in which the major compounds were acylated pelargonidin derivatives, were assessed with a variety of assays in vitro. the extract appeared to form a complex with fe(3+) or cu(2+). it displayed a concentration-dependant reducing power (1.16od(700 nm) at a concentration of 4mm) and scavenging effect against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radicals (with ic(50)=1.74 +/- 0.03 mm). it could promote the cle ... | 2010 | 20600520 |
| immunoassay for quantifying squamous cell carcinoma antigen in serum. | although the benefits of quantifying serum squamous cell carcinoma antigen (scca) have been reported, scca reagents were no longer available in the us by the late 1990s. because scca quantification still has demonstrated clinical utility, we developed and validated a microtiter plate-based elisa for measuring scca in serum. | 2010 | 20601447 |
| pre-embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectin. | in histochemistry and cytochemistry, horseradish peroxidase-labeled lectins are often used as probes for the localization of carbohydrates in cells and tissues. in transmission electron microscopy, the most commonly used procedure for detection of carbohydrates is lectin-gold labeling. horseradish peroxidase catalyzes the formation of insoluble polymerized diaminobenzidine which on exposure to osmium tetroxide forms osmium black, a compound visible in the electron microscope, making horseradish ... | 2010 | 20602219 |
| horseradish peroxidase as a reporter gene and as a cell-organelle-specific marker in correlative light-electron microscopy. | a modern electron microscopic approach to the investigation of the structural organization of proteins and subcellular structures demands the use of molecular genetic techniques. the successful implementation of genetic techniques is closely tied to a reporter gene such as the green fluorescent protein (gfp). although gfp has been widely used for light microscopy, it has many limitations for use in electron microscopy. in the search for a reporter gene for electron microscopy, interest in the us ... | 2010 | 20602227 |
| a sensitive choline biosensor with supramolecular architecture. | a sensitive biosensor with supramolecular architecture was designed and implemented here to detect choline. choline oxidase and horseradish peroxidase were assembled onto the polymer of thiolated beta-cyclodextrin and platinum nanoparticles modified gold electrode through 1-adamantane carboxylic acid coupling. square wave voltammetry showed that the reduction currents at 0.38 mv had a linear relationship with the logarithms of choline concentrations in the range of 10(-9)-10(-2) m, and the detec ... | 2010 | 20602924 |
| real-time monitoring and kinetic parameter estimation of the affinity interaction of jartinm and rartinm with peroxidase glycoprotein by the electrogravimetric technique. | artinm is a d-mannose binding lectin that has been arousing increasing interest because of its biomedical properties, especially those involving the stimulation of th1 immune response, which confers protection against intracellular pathogens. the potential pharmaceutical applications of artinm have motivated the production of its recombinant form (rartinm) so that it is important to compare the sugar-binding properties of jartinm and rartinm in order to take better advantage of the potential app ... | 2010 | 20605432 |
| spinal motor neuronal degeneration after knee joint immobilization in the guinea pig. | this study used a unilateral knee joint immobilization model in adult guinea pigs to test the hypothesis that retrograde degeneration of motor neurons in the spinal cord is the result of attenuation of knee joint activities. | 2010 | 20605551 |
| hairpin dna switch for ultrasensitive spectrophotometric detection of dna hybridization based on gold nanoparticles and enzyme signal amplification. | a novel dna detection platform based on a hairpin dna switch, nanoparticles, and enzyme signal amplification for ultrasensitive detection of dna hybridization has been developed in this work. in this dna assay, a "stem-loop" dna probe dually labeled with a thiol at its 5' end and a biotin at its 3' end, respectively, was used. this probe was immobilized on the gold nanoparticles (aunps) anchored by a protein, gamma-globulin, on a 96-well microplate. in the absence of target dna, the immobilized ... | 2010 | 20608643 |
| determination of l-tyrosine based on luminescence quenching of mn-doped znse quantum dots in enzyme catalysis system. | in this paper, we attempted to develop a novel application of mn-doped znse quantum dots (mn: znse d-dots) as probes to detect l-tyrosine (l-tyr). the bio-conjugates of horseradish peroxidase (hrp)-conjugated mn: znse d-dots were used in the enzyme catalyzed reaction of l-tyr with h(2)o(2.) compared with traditional cdte qds, mn: znse d-dots have better biocompatibility and less negative impact on enzyme catalyzed system. in hrp-conjugated mn: znse-l-tyr-h(2)o(2) system, electron transfer occurr ... | 2011 | 20614168 |
| detection of six genetically modified maize lines using optical thin-film biosensor chips. | as more and more genetically modified organisms (gmo) are commercialized, efficient and inexpensive assays are required for their quick detection. an event-specific detection strategy based on the unique and specific sequences of integration junctions is useful because of its high specificity. this study developed a system for detecting six gm maize lines (bt11, bt176, ga21, mon810, nk603, and t25) using optical silicon thin-film biosensor chips. aldehyde-labeled probes were arrayed and covalent ... | 2010 | 20614904 |
| controlling thermo-reversibility of gelatin gels through a peroxidase-catalyzed reaction under mild conditions for mammalian cells. | a variety of cross-linking methods is used for obtaining gelatin gels having a tolerance to thermo-reversible gel-sol transition at physiological temperature. in this paper, we investigated the applicability of horseradish peroxidase-catalyzed cross-linking of tyrosine residues originally contained in native gelatin molecules for preparing such gelatin gels. the gelatin gels obtained through exposure to the enzymatic reaction showed a higher resistance to thermo-reversibility at 37°c than gels o ... | 2011 | 20615328 |
| oxidative decarboxylation of tris-(p-carboxyltetrathiaaryl)methyl radical epr probes by peroxidases and related hemeproteins: intermediate formation and characterization of the corresponding cations. | tris(p-carboxyltetrathiaaryl)methyl radicals (tam*) are good epr probes for measurement of dioxygen concentration in biological systems and for epr imaging. it has been previously reported that these radicals are efficiently oxidized by superoxide, o2*(-), or alkylperoxyl radicals, roo*, and by liver microsomes via an oxidative decarboxylation mechanism leading to the corresponding quinone-methides (qm). this article shows that peroxidases, such as horseradish peroxidase (hrp), lactoperoxidase ( ... | 2010 | 20615385 |
| recombinant peroxidase production in species of lepidoptera frequently found in argentina. | horseradish peroxidase isozyme c (hrpc) is an important commercial biocatalyst. in this study, a screening of different lepidopteran species frequently found in argentina to produce this protein was carried out. two recombinant viruses were constructed: acmnpv hrpc polyhedrin-minus (occ-), an intrahemocelical infective virus; and acmnpv hrpc polyhedrin-plus (occ+), to achieve an oral infective baculovirus. each lepidopteran species was infected either with acmnpv hrpc occ- or acmnpv hrpc occ+ an ... | 2010 | 20615485 |
| arabidopsis peroxidase-catalyzed copolymerization of coniferyl and sinapyl alcohols: kinetics of an endwise process. | in order to determine the mechanism of the earlier copolymerization steps of two main lignin precursors, sinapyl (s) alcohol and coniferyl (g) alcohol, microscale in vitro oxidations were carried out with a prx34 arabidopsis thaliana peroxidase in the presence of h(2)o(2). this plant peroxidase was found to have an in vitro polymerization activity similar to the commonly used horseradish peroxidase. the selected polymerization conditions lead to a bulk polymerization mechanism when g alcohol was ... | 2010 | 20615517 |
| disposable amperometric magnetoimmunosensors for the specific detection of streptococcus pneumoniae. | disposable amperometric magnetoimmunosensors, based on the use of functionalized magnetic beads and gold screen-printed electrodes, have been developed for the selective detection and quantification of streptococcus pneumoniae. a specific antibody prepared against a serotype 37 s. pneumoniae strain, selected by flow cytometry among seven anticapsular or antisomatic antibodies, was linked to protein a-modified magnetic beads and incubated with bacteria. the same antibody, conjugated with horserad ... | 2010 | 20615685 |
| histochemical evaluation of human prostatic tissues with cratylia mollis seed lectin. | lectins, proteins which selectively recognize carbohydrates, have been used in histochemistry for the evaluation of changes in glycosylation in processes of cellular differentiation and/or dedifferentiation. cratylia mollis seed lectins (cramoll 1,4 and cramoll 3), conjugated to horseradish peroxidase, were used as histochemical probes in human prostate tissues: normal (np), hyperplasia (bph), and prostate carcinoma (pca). the staining pattern of con-a and cramoll 1,4 in bph was more intense tha ... | 2010 | 20617135 |
| ph-controllable on-off bioelectrocatalysis of bienzyme layer-by-layer films assembled by concanavalin a and glucoenzymes with an electroactive mediator. | mediated electrochemical biosensors consisting of two enzymes have attracted increasing interest because of their wider applicability. in this work, concanavalin a (con a) and two glycoenzymes, horseradish peroxidase (hrp) and glucose oxidase (god), were assembled into {con a/hrp/con a/god}(n) layer-by-layer films on an electrode surface mainly by lectin-sugar biospecific interaction between con a and glycoenzymes. the cyclic voltammetry (cv) response of fe(cn)(6)(3-) at the bienzyme film electr ... | 2010 | 20617850 |
| montelukast treatment (cysteinyl leukotriene receptor antagonist) in a model of food allergy: modifications in lymphatic cell population from rectal mucosa. | the aim is to determine immunopathological modifications in rectal mucosa from rabbits after local challenge in ovalbumin (ova) sensitized animals previously treated with montelukast. | 2010 | 20617862 |
| noncompetitive immunoassay for follicle-stimulating hormone in human serum using capillary electrophoresis with chemiluminescence detection. | a noncompetitive immunoassay based on capillary electrophoresis (ce) with chemiluminescence (cl) detection has been developed for the determination of follicle-stimulating hormone (fsh) in human serum. the work involved the development of separation and cl conditions allowing for routine analysis of serum samples. in this study, horseradish peroxidase (hrp)-labeled monoclonal anti-fsh can catalyze the luminol-hydrogen peroxide reaction. the determined fsh can react with an excessive amount of hr ... | 2010 | 20623406 |
| online coupling of reverse-phase and hydrophilic interaction liquid chromatography for protein and glycoprotein characterization. | we have developed a novel system for coupling reverse-phase (rp) and hydrophilic interaction liquid chromatography (hilic) online in a micro-flow scheme. in this approach, the inherent solvent incompatibility between rp and hilic is overcome through the use of constant-pressure online solvent mixing, which allows our system to perform efficient separations of both hydrophilic and hydrophobic compounds for mass spectrometry-based proteomics applications. when analyzing the tryptic digests of bovi ... | 2010 | 20632160 |
| oligonucleotide microarrays with horseradish peroxidase-based detection for the identification of extended-spectrum β-lactamases. | production of extended-spectrum β-lactamases (esbls) is the one of most widespread and clinically significant mechanism of enterobacteriaceae resistance towards modern β-lactam antibiotics. there are known 400 esbls, with the majority represented by the enzymes of tem, shv and ctx-m families. oligonucleotide microarrays with colorimetric detection have been developed for the purposes of determination of esbls and inhibitor-resistant β-lactamases using horseradish peroxidase (hrp). specific oligo ... | 2010 | 20643540 |
| bias-dependent photocurrent response in protein nanolayer-embedded solid state planar diode devices. | a bias-dependent superlinear photocurrent response under white light illumination was measured in the planar diodes with a protein nanolayer of horseradish peroxidase (hrp) that is a key material for sensing hydrogen peroxide in biological systems. | 2010 | 20648312 |
| possible roles of phospholipase a(2) in the biological activities of acanthamoeba castellanii (t4 genotype). | using phospholipases a(2)-specific spectrophotometric assays, it was shown thata. castellaniilysates and their conditioned medium exhibit phospholipase activities. the extracellular levels of pla(2)detected were significantly reduced compared with the cell-associated enzyme (p<0.05). sphinganine, a pla(2)inhibitor showed robust amoebistatic properties but had no effect on the viability ofa. castellanii. the potency of sphinganine was demonstrated effectively towards purified pla(2)derived from p ... | 2011 | 20650684 |
| transformation of dissolved organic matter by oxidative polymerization with horseradish peroxidase. | dissolved organic matter (dom) has significant influence on the transport and fate of contaminants in multiple phases and it has potential hazard by acting as a precursor of disinfection by-products in water supply. the changes in dom characteristics, especially by oxidative polymerization might result in different behaviour in the interaction with many contaminants. the aim of this work was to verify the catalytic effects of peroxidase on oxidative polymerization of humic and fulvic substances ... | 2010 | 20651438 |