Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| molecular subtyping of vibrio cholerae o1 strains recently isolated from patient, food and environmental samples in spain. | nineteen vibrio cholerae o1 strains isolated in spain from patient, food and environmental samples in the period 1990-1992 were characterized by detection of cholera toxin by enzyme immunoassay, detection of cholera toxin gene by polymerase chain reaction, and by biotyping, ribotyping and pulsed-field gel electrophoresis. ten isolates were toxigenic and were further characterized by multilocus enzyme electrophoresis. molecular subtyping methods allowed precise differentiation between isolates, i ... | 1994 | 8070433 |
| identification of vcr, a repeated sequence associated with a locus encoding a hemagglutinin in vibrio cholerae o1. | we have determined the nucleotide sequence of a 6.3-kb bamhi fragment of the chromosome of vibrio cholerae 569b that includes the sequence of the mannose-fucose-resistant hemagglutinin reported previously (v.l. franzon, a. barker, and p. a. manning, infect. immun. 61:3032-3037, 1993). this region contains nine copies of a 124-bp direct repeat, here named vcr, of imperfect dyad symmetry, that are shown by southern hybridization to occur at least 60 to 100 times in the v. cholerae o1 chromosome. l ... | 1994 | 8071223 |
| international dissemination of epidemic vibrio cholerae by cargo ship ballast and other nonpotable waters. | in 1991 and 1992, toxigenic vibrio cholerae o1, serotype inaba, biotype el tor, was recovered from nonpotable (ballast, bilge, and sewage) water from five cargo ships docked in ports of the u.s. gulf of mexico. four of these ships had taken on ballast water in cholera-infected countries; the fifth took on ballast in a noninfected country. isolates examined by pulsed-field gel electrophoresis were indistinguishable from the latin american epidemic strain, c6707; however, they differed significant ... | 1994 | 8074532 |
| the relationship between abo blood groups and susceptibility to diarrhea due to vibrio cholerae 0139. | 1994 | 8075282 | |
| problems and prospects of a traditional source of potable water in hilly terrains. | during epidemic investigation, water sources were tested for quality for drinking purpose. out of 30 khatris tested, water of 86.7% khatris was fit for human consumption and fecal coliform was not found in any of them. modification of these khatris was stressed by respondents. | 1993 | 8076998 |
| development and evaluation of rapid monoclonal antibody-based coagglutination test for direct detection of vibrio cholerae o139 synonym bengal in stool samples. | a monoclonal antibody-based coagglutination test directly detected vibrio cholerae o139 synonym bengal in 83 of 120 watery diarrheal stool specimens; on culture, 90 samples were positive. thus, with 92% sensitivity, 100% specificity, and 100% positive and 95% negative predictive values, the coagglutination test is a useful rapid test for v. cholerae o139. | 1994 | 8077410 |
| the novel epidemic strain o139 is closely related to the pandemic strain o1 of vibrio cholerae. | a new vibrio cholerae serogroup o139 strain of unknown origin recently emerged in india and bangladesh, causing a major outbreak of cholera. the genetic relationship between this epidemic strain and the o1 strain responsible for the 7th pandemic of cholera was studied by analyzing the dna polymorphism of v. cholerae by pulsed-field gel electrophoresis and arbitrarily primed polymerase chain reaction. the restriction patterns of the reference strain o139 bengal and 10 wild o139 strains isolated e ... | 1994 | 8077733 |
| distribution of the ace, zot, and ctxa foxin genes in clinical and environmental vibrio cholerae. | 1994 | 8077743 | |
| [the determination of the toxigenicity of vibrio cholerae strains by using a culture of chinese hamster ovary cells]. | 1993 | 8079536 | |
| localization of vibrio cholerae o1 in the intestinal tissue. | colonization of v. cholerae o1 in vivo is known to be a non-invasive type which the vibrios are confined only to the intestinal tissues. the pathway by which the vibrio antigens reach the lymphoid cells and subsequently give rise to the immune responses is not entirely clear. thus, experiments were performed in experimental rats by inoculating live v. cholerae o1 into the ligated ileal loops. the fate of the vibrios in the intestinal tissues was then studied by transmission electron microscopy a ... | 1993 | 8080608 |
| [protection of vibrio cholerae from heating and chlorination by chitin]. | el tor vibrio cholerae and non-01 v. cholerae absorbed onto chitin particles could not only multiply in vitro, but also could partly survive with heating at 65-75 degrees c for 60-160 sec (7/40) or with 3.8-15 ppm of effective chlorine for 10 min (24/29). none of those unabsorbed onto chitin could survive at the above temperature, and only very few of those could survive with the above concentration of effective chlorine. these findings are conducive to the control of cholera transmission caused ... | 1994 | 8082453 |
| identification, cloning, and sequencing of a gene required for ferric vibriobactin utilization by vibrio cholerae. | chromosomal dna downstream of the vibrio cholerae ferric vibriobactin receptor gene, viua, was cloned and sequenced, revealing an 813-bp open reading frame encoding a deduced protein of 271 amino acids. in vitro transcription-translation of this dna confirmed expression of a protein of the expected size. a deletion mutation of this gene, viub, was created in the classical v. cholerae strain o395 by in vivo marker exchange. by cross-feeding studies, this mutant was unable to utilize exogenous fer ... | 1994 | 8083157 |
| vibrio cholerae o139 bengal possesses a capsular polysaccharide which may confer increased virulence. | a newly described vibrio cholerae serogroup--o139 bengal, the causative agent of the recent large epidemics of cholera-like disease in the indian subcontinent and neighbouring countries--possesses a high molecular weight capsular polysaccharide (cps) that can be visualized by electron microscopy and in composition differs from the lipopolysaccharide (lps). the cps and lps can be separated from each other by a two-step extraction procedure, a phenol-water extraction in order to extract all polysa ... | 1994 | 8090081 |
| tetracycline resistant vibrio cholerae in pilgrims returning from mecca. | 1994 | 8090106 | |
| an analysis of the v1 and v2 regions of vibrio cholerae and vibrio mimicus 16s rrna. | the v1 and v2 variable regions of the 16s rrna gene of three strains of v. cholerae and one strain of v. mimicus were amplified by pcr. fragments containing both regions were cloned into m13mp18 using smal and sequenced by the dideoxy method. the 263-bp sequence from a strain isolated during the 1991 cholera outbreak in brazil was deposited in genbank under the accession number l05178. except for an extra g in one of the strains, the three v. cholerae sequences were identical. the v. mimicus seq ... | 1994 | 8090995 |
| summary of the 27th united states-japan joint conference on cholera and related diarrheal diseases. | 1993 | 8093361 | |
| biophysical characterization of vibrio el tor typing phage e5. | vibrio cholerae typing phage e5, which can lyse only the el tor strains of v. cholerae, was characterized. the phage had a polyhedral head 51 nm in diameter and a short tail 13 nm in length. it contained 13 structural polypeptides, with the molecular mass of the major component being 50 kda. phage chromosome comprised a 38.5-kb linear double-stranded dna molecule with unique termini, as determined by restriction fragment analysis and electron microscopy, and had a g+c content of 35.5%. a physica ... | 1993 | 8095039 |
| emergence of novel strain of vibrio cholerae with epidemic potential in southern and eastern india. | 1993 | 8095620 | |
| large outbreak of clinical cholera due to vibrio cholerae non-o1 in bangladesh. | 1993 | 8095621 | |
| polymyxin b sensitive strains of vibrio cholerae non-o1 from recent epidemic in india. | 1993 | 8096977 | |
| major shift in prevalence of non-o1 and el tor vibrio cholerae. | 1993 | 8096978 | |
| biogenesis and regulation of the vibrio cholerae toxin-coregulated pilus: analogies to other virulence factor secretory systems. | biogenesis of the toxin-coregulated pilus (tcp) of vibrio cholerae 01 is essential for successful bacterial colonization of the small intestine. pilus assembly requires the products of at least seven genes located on the chromosome adjacent to the pilin-encoding gene, tcpa. previously reported tnphoa insertions in the tcp-assembly-deficient v. cholerae strains, kp2.21 and kp4.2, were isolated from the chromosome for further analysis. nucleotide sequencing of the tcpe::phoa and tcpf::phoa fusions ... | 1993 | 8097177 |
| genetic organization and sequence of the promoter-distal region of the tcp gene cluster of vibrio cholerae. | the nucleotide sequence of the promoter distal region of the major operon associated with biosynthesis of tcp, the toxin coregulated pilus of vibrio cholerae has been determined. the genes tcpr, tcpd, tcps, tcpt, tcpe and tcpf are organized to permit translational coupling and are followed by an inverted repeat structure which is likely to act as a strong rho-independent terminator. tcps and tcpf possess n-terminal signal sequences and would be expected to be periplasm and outer membrane located ... | 1993 | 8097178 |
| pili of vibrio cholerae widely distributed in serogroup o1 strains. | the distribution of vibrio cholerae o1 pili consisting of 16 kda subunit protein (16k-pili) was examined by western blotting, using 211 strains from various origins and specific anti-16k-pili sera. the 16 kda protein was detected in all 211 strains. the pili were purified from 3 el tor and 3 classical strains, and characterized by hemagglutination and inhibition tests. all purified pili were hemagglutinative. however, the hemagglutinating activity of classical pili disappeared after exposure to ... | 1993 | 8097272 |
| antibody cross-reactions with lipopolysaccharide from e. coli o157 after cholera vaccination. | 1993 | 8098423 | |
| outbreak of vibrio cholerae non-o1 in india and bangladesh. | 1993 | 8098474 | |
| detecting and biotyping vibrio cholerae o1 with multiplex polymerase chain reaction. | 1993 | 8100020 | |
| vibrio cholerae non-o1--the eighth pandemic? | 1993 | 8101894 | |
| large epidemic of cholera-like disease in bangladesh caused by vibrio cholerae o139 synonym bengal. cholera working group, international centre for diarrhoeal diseases research, bangladesh. | epidemics of cholera caused by vibrio cholerae o1 occur regularly in bangladesh, but until lately v cholerae non-o1 has been associated only with sporadic cases of diarrhoeal disease in many parts of the world, including bangladesh. we describe a large epidemic of cholera-like disease in bangladesh that is due to a v cholerae non-o1. the epidemic began in december, 1992, in southern bangladesh and spread throughout the country. by the end of march 107,297 cases of diarrhoea and 1473 deaths had b ... | 1993 | 8101899 |
| isolation of vibrio cholerae o139 bengal from water in bangladesh. | 1993 | 8101919 | |
| non-o1 vibrio cholerae. | 1993 | 8101920 | |
| vibrio cholerae o139 bengal in bangkok. | 1993 | 8101921 | |
| blood stream invasion by vibrio cholerae o139. | 1993 | 8101922 | |
| bacteremic cellulitis caused by non-o1 vibrio cholerae: report of a case. | a 44-year-old man presented with a fever of a two-day duration and bullous cellulitis of the lower extremities. he had prepared and eaten saltwater fish three days prior to admission. both blood and bullous fluid cultures showed non-o1 vibrio cholerae. the bacteremic cellulitis resolved gradually after four weeks of intravenous cefamandole therapy and surgical debridement. non-o1 v. cholerae bacteremia is rarely reported in the english-language literature and is almost always associated with sal ... | 1993 | 8104603 |
| a 20-kda pilus protein with haemagglutination and intestinal adherence properties expressed by a clinical isolate of non-01 vibrio cholerae. | a clinical isolate of non-01 v. cholerae (10325) was shown to exhibit higher haemagglutination and intestinal adherence activities in vitro when grown in enriched media, such as trypticase soy broth (tsb) as compared to those of cells grown in a synthetic tris-buffered or 't'-medium. a comparison of their cell-surface protein and lipopolysaccharide profiles suggested the involvement of a 20-kda protein in the cellular adherence process. an antiserum, raised specifically against the 20-kda protei ... | 1993 | 8104843 |
| cholera isolates in relation to the "eighth pandemic". | 1993 | 8105182 | |
| cholera isolates in relation to the "eighth pandemic". | 1993 | 8105183 | |
| cholera isolates in relation to the "eighth pandemic". | 1993 | 8105184 | |
| cooperative studies on diarrheal diseases. | enteric infection still causes the highest global morbidity and mortality in children under 5 years old. while there have been impressive developments in the early treatment of diarrhea, adequate diagnostic techniques are not yet available. an international collaboration was conducted with the national institute of cholera and enteric diseases, calcutta on the rapid diagnosis and prevention of diarrheal diseases. forty to fifty per cent of the 780-bed infectious diseases hospital is occupied by ... | 1993 | 8109246 |
| electron microscopic study of phages and aberrant structures produced by induction of prophage kappa in vibrio cholerae el tor cells. | prophage kappa in v. cholerae el tor strain slh22(j) could be induced spontaneously or by treatment with nitrofurantoin, though the efficiency of induction was very low (not more than 0.8%). v. cholerae el tor cells were found to release many different aberrant structures of the temperate phage, kappa. these aberrant structures were characterized by density gradient centrifugation and electron microscopy. | 1993 | 8112775 |
| oral administration of polymeric immunoglobulin a prevents colonization with vibrio cholerae in neonatal mice. | a simple animal model was used to demonstrate passive protection by immunoglobulin a (iga) against a mucosal pathogen, vibrio cholerae. oral administration of a monoclonal iga directed against a lipopolysaccharide component of the vibrio protected neonatal mice against oral challenge, as measured by reduced intestinal colonization. a single dose of 0.1 microgram of polymeric monoclonal iga given 1 h prior to challenge reduced the number of recoverable vibrios by at least 100-fold. an additional ... | 1994 | 8112859 |
| vibrio mimicus with multiple toxin types isolated from human and environmental sources. | a collection of 13 strains of vibrio mimicus, including both clinical and environmental isolates from different geographic regions, was examined for various toxins. one strain of environmental origin produced cholera-like toxin (ct) which was completely absorbed with anti-ct immunoglobulin g, five strains produced a haemolysin that cross-reacted with the thermostable direct haemolysin of v. parahaemolyticus and dna from two strains hybridised with a dna probe specific for the heat-stable enterot ... | 1994 | 8114069 |
| vibrios among patients of good socioeconomic conditions during the cholera epidemic in recife, brazil. | between march and july, 1992, we screened for vibrio all fecal samples submitted for bacteriologic diagnosis at a private clinical laboratory in recife. of 1435 cultures examined only 1 (0.07%) was positive for v. cholerae 01, biovar eltor, serovar inaba, but 17 (1.2%) yielded non-cholera vibrio (v. cholerae non-01; v. fluvialis; v. furnissii, v. parahaemolyticus and vibrio spp). thus, v. cholerae 01, differently of other enteropathogenic vibrios, spared individuals of good socioeconomic conditi ... | 1993 | 8115794 |
| epidemic cholera in ecuador: multidrug-resistance and transmission by water and seafood. | to determine risk factors for cholera in an epidemic-disease area in south america, a case-control investigation was performed in guayaquil, ecuador, in july 1991. residents > 5 years old who were hospitalized for treatment of acute, watery diarrhoea and two matched controls for each were interviewed regarding sources of water and food, and eating, drinking, and hygienic habits. interviewers inspected homes of case-patients and controls to document water treatment, food-handling, and hygienic pr ... | 1994 | 8119348 |
| cholera in the united states, 1965-1991. risks at home and abroad. | to assess risks for cholera in the united states. | 1994 | 8122948 |
| [bacteriological aspects of cholera in benin (epidemic of 1991)]. | this study showed that the strains of vibrio cholerae el tor, ogawa serotype, isolated during the last cholera outbreak in benin (1991) are widely sensitive to tetracyclin (84%), sulfamid (96%), ampicillin (98%). | 1993 | 8124099 |
| the dna adenine methyltransferase-encoding gene (dam) of vibrio cholerae. | the dna adenine methyltransferase (mtase)-encoding gene (dam) of vibrio cholerae, an organism belonging to the family vibrionaceae, has been cloned and the complete nucleotide (nt) sequence determined. v. cholerae dam encodes a 21.5-kda protein and is directly involved in methyl-directed dna mismatch repair. it can substitute for the escherichia coli enzyme and can suppress the phenotypic traits associated with e. coli dam mutants. overproduction of v. cholerae dam mtase does not result in hyper ... | 1994 | 8125341 |
| [a study of the biological properties of cholera phages and strains of el-tor vibrions isolated from the external environment in donetsk]. | choleric phages were identified in 2 out of 44 samples of donetsk sewage. biological properties of phages were characterized. | 1993 | 8125357 |
| a novel kit for rapid detection of vibrio cholerae o1. | we report on the development and testing of a novel, rapid, colorimetric immunodiagnostic kit, cholera smart, for direct detection of the presence of vibrio cholerae o1 in clinical specimens. unlike conventional culture methods requiring several days to complete, the cholera smart kit can be used directly in the field by untrained or minimally skilled personnel to detect v. cholerae o1 in less than 15 min, without cumbersome laboratory equipment. a total of 120 clinical and environmental bacteri ... | 1994 | 8126193 |
| detection and identification of toxigenic vibrio cholerae o1 strains by a simplified polymerase chain reaction method. | the polymerase chain reaction (pcr) with three oligonucleotide primers was used to amplify both 184 bp and 425 bp dna fragments of cholera toxin a subunit gene (ctxa) for detection and identification of toxigenic v. cholerae o1 strains in one step. for enhancing the sensitivity, we have used the chemiluminescent detection system for southern hybridization. a total of 92 vibrio strains were tested by both pcr and enzyme-linked immunosorbent assay (elisa). three e. coli (etec) strains, one toxigen ... | 1993 | 8131662 |
| potential for reacquisition of cholera enterotoxin genes by attenuated vibrio cholerae vaccine strain cvd 103-hgr. | the potential for reacquisition of ctxa genes by attenuated vibrio cholerae o1 vaccine strain cvd 103-hgr was examined by performing a series of mating experiments under a variety of in vivo and in vitro conditions. we found no evidence that cvd 103-hgr could reacquire ctxa genes from wild-type v. cholerae o1 strains. however, if the donor v. cholerae o1 strains were genetically manipulated to add genes that allow chromosomal gene transfer, then ctxa sequences could be acquired by cvd 103-hgr. t ... | 1994 | 8132356 |
| characterization of vibrio cholerae o139 synonym bengal isolated from patients with cholera-like disease in bangladesh. | vibrio cholerae o139 (synonym bengal), a novel serovar of v. cholerae, is the causative agent of large outbreaks of cholera-like illness currently sweeping india and bangladesh. eight randomly selected v. cholerae o139 isolates were studied for their biological properties, which were compared with those of v. cholerae o1 and other v. cholerae non-o1. the v. cholerae o139 isolates were characterized by the production of large amount of cholera toxin, hemagglutination, weak hemolytic properties, r ... | 1993 | 8133802 |
| comparative study of two transport systems for vibrio cholerae. | 1994 | 8134943 | |
| analysis of some factors involved in the virulence mechanism of vibrio cholerae non-01. | a study was carried out to evaluate the potential intestinal toxicity of 188 samples of vibrio cholerae non-01 isolated from seawater found along the beaches of rio de janeiro city. three different assays were carried out involving: (a) detection of vascular permeability factor (pf) in guinea pigs (together with assessment of two culture media for production of the toxin); (b) intestinal fluid accumulation (fa) in suckling mice; and (c) detection of haemolysin. the results demonstrated that both ... | 1993 | 8136780 |
| the effect on enterotoxicity of protease purified from vibrio cholerae o1. | the effect on enterotoxicity of protease purified from vibrio cholerae o1 was investigated by the inoculation of live vibrio cells into protease-treated loops of the ileal loop model. fluid accumulation ratios in the protease-treated loops were elevated in a dose-dependent manner by challenge with live vibrio cells but not by that with toxin. an enhancement effect of protease on enterotoxicity was observed in both serotypes of v. cholerae o1 and v. cholerae non-o1. it is suggested, therefore, th ... | 1994 | 8138138 |
| [the first report of traveler's diarrhea associated with a newly described toxigenic vibrio cholerae o139 strain in japan]. | a newly described vibrio cholerae o139 was isolated from a patient who had traveled in india on april 1993. the patient experienced 5 to 6 watery diarrhea per day after he returned to japan. the isolated strain registered as k111 did not agglutinate with o1-o138 antiserum and agglutinated with o139 antiserum. this strain resembled v. cholerae o1 strain in biochemical characters and had ctx and zot, although was resistant to the vibrio static agent o/129. this is the first report of cholera-like ... | 1994 | 8138681 |
| determination of sialic acids. | 1994 | 8139495 | |
| [cholera immunology and the molecular biology of cholera toxin. recent progress and future prospects]. | cholera toxin (ct) and the analogous heat-labile enterotoxin (lt) from escherichia coli have several immunomodulating effects that might explain their adjuvant action in stimulating secretory mucosal iga after oral immunization. in mice experimental model, these effects include: enhanced antigen presentation by macrophages and other cell types; promotion of isotype differentiation in b cells leading to increased iga formation; and other important effects on t cell proliferation and lymphokine pr ... | 1993 | 8143024 |
| use of genetic recombination as a reporter of gene expression. | an understanding of the patterns of gene expression in response to specific environmental signals can yield insight into a variety of complex biological systems such as microbial-host interactions, developmental cycles, cellular differentiation, ontogeny, etc. to extend the utility of the reporter gene fusion approach to such studies, we have constructed a gene expression reporter cassette that permits the generation of transcriptional fusions to tnpr encoding resolvase, a site-specific recombin ... | 1994 | 8146167 |
| influence of animal passage on haemolysin and enterotoxin production in vibrio cholerae o1 biotype el tor strains. | of 43 strains of vibrio cholerae o1 biotype el tor isolated over a span of almost three decades (1964-1990) from stools of children and adults with diarrhoea (25 isolates) and from sewage (three) and water from the river ganges (15) examined for production of haemolysin and its correlation with enterotoxin production, 17 isolates showed haemolysis. the majority of isolates (26), including 68% of diarrhoeal and 50% of environmental origin, were non-haemolytic. the titre of haemolysin produced was ... | 1994 | 8151674 |
| surveillance of cholera due to vibrio cholerae o139. | 1994 | 8155519 | |
| algal blooms in the spread and persistence of cholera. | cholera has been long associated with the seasonality of coastal algal blooms off bangladesh. using fluorescent antibody (fa) techniques, microbiologists have now identified a viable, non-cultivable form of vibrio cholerae in a wide range of marine life, including cyanobacteria (anabaena variabilis), diatoms (skeletonema costatum), phaeophytes (ascophyllum nodosum), in copepod molts, and in freshwater vascular aquatic plants (water hyacinths and duckweed). in unfavourable conditions v. cholerae ... | 1993 | 8155853 |
| selective regulation of chemotactic lymphokine production by monocytes and macrophage cell line cells. | effects of lipopolysaccharides (lps) on the production of chemotactic lymphokines for eosinophils and monocytes (ecf and mcf) from antigen- or mitogen-stimulated t cells were examined. supernatants from monocytes stimulated with various lps regulated concanavalin a (con a)- and purified protein derivative (ppd)-induced ecf or mcf production. the regulation varied with lps used for monocyte stimulation. the supernatant from monocytes stimulated with lps from escherichia coli, j-5 strain, selectiv ... | 1994 | 8155994 |
| enteropathogenic bacteria in river ganges in varanasi. | a bacteriological study of the river ganges in varanasi was carried out from december 1985 to november 1987. in all 407 water samples were collected, 335 from bathing ghats and 72 from sewage openings and were examined for the presence of enteropathogenic bacteria. vibrio cholerae 0-1 (1.72%), non 0-1 vibrio cholera (3.69%), vibrio fluvialis (0.74%), aeromonas sp. (0.49%), plesiomonas sp. (0.25%), salmonella sp. (0.98%) and shigella sp. (1.23%) were isolated from both ghats and sewage openings a ... | 1993 | 8157311 |
| new type of cholera. | 1993 | 8157330 | |
| cutaneous manifestations of non-01 vibrio cholerae septicemia with gastroenteritis and meningitis. | a 58-year-old man with diabetes had fever and chills 5 days after ingestion of raw seafood. nausea, vomiting, watery diarrhea, bilateral calf pain, and neck stiffness subsequently developed. generalized edema and ecchymotic patches with a vesiculobullous eruption appeared on the extremities. four blood cultures were positive for vibrio cholerae non-01. the patient was successfully treated with antibiotics. this is the first documented case of v. cholerae non-01 septicemia with cutaneous lesions ... | 1994 | 8157789 |
| imported cholera in a 31-year-old peruvian female. | we present the case of a 31-year-old peruvian female with severe dehydration due to diarrhea and vomiting. the patient was one of a number of travelers arriving in los angeles on an international flight from argentina and peru. because of the travel history and clinical presentation, cholera was suspected and ultimately confirmed by stool culture. the patient's clinical course is outlined, and discussion of the relevant epidemiology and clinical management of cholera is provided. physicians shou ... | 1993 | 8157909 |
| the etiology of early childhood diarrhea: a community study from guinea-bissau. | a potential enteropathogen was found in 50% of 1219 diarrheal episodes and 48% of 511 asymptomatic controls in a 1-year community study of childhood diarrhea. rotavirus (3% of episodes), cryptosporidium species (6%), and enteropathogenic escherichia coli (epec) with epec adherence factor (4%) were more prevalent in cases than controls. giardia lamblia (19%) was the most prevalent organism but was not associated with diarrhea. enterotoxigenic e. coli (12%), strongyloides stercoralis (5%), shigell ... | 1994 | 8158030 |
| lack of cross-protection against diarrhea due to vibrio cholerae o1 after oral immunization of rabbits with v. cholerae o139 bengal. | 1994 | 8158063 | |
| multiple regulatory systems in vibrio cholerae pathogenesis. | 1994 | 8162438 | |
| vibrio cholerae o139 synonym bengal is closely related to vibrio cholerae el tor but has important differences. | although vibrio cholerae o139 synonym bengal strains, from the current epidemics in india and bangladesh, are closely related to seventh-pandemic strains, as shown by multilocus enzyme electrophoresis, bengal strains are encapsulated and portions of the o1 antigen biosynthetic complex genes found in o1 strains are altered or lacking. encapsulated bengal strains showed resistance to killing by normal human serum. the presence of the capsule suggests the potential for bloodstream invasion in susce ... | 1994 | 8168977 |
| phage psi 92: a new choleraphage infecting vibrio cholerae cells belonging to serovars 01 and non-01. | a new choleraphage, psi 92, with an icosahedral head and no tail structure has been described. this phage has a wide host range. it infects and lyses strains belonging to different serovars and biotypes of vibrio cholerae. the phage utilises host dna degradation products for its own dna synthesis. psi 92 contains a linear double-stranded dna of 56 kbp. a physical map of the phage genome has been constructed using the restriction enzyme kpni. intracellular development of phage psi 92 following in ... | 1993 | 8169115 |
| spread of vibrio cholerae o139 bengal in india. | vibrio cholerae serogroup o139 bengal, a novel strain with epidemic potential, completely displaced v. cholerae serogroup 01 in calcutta in january 1993, which was followed by an epidemic caused by v. cholerae o139 in march-may 1993. from november 1992 to july 1993, 95.6% of 916 v. cholerae isolates submitted to the national institute of cholera and enteric diseases from 28 locations in india were confirmed as serogroup o139. as of july 1993, v. cholerae o139 had been isolated from 13 indian sta ... | 1994 | 8169387 |
| seawater effects on various vibrio species. | this study compared the effects of sea water on vibrio cholerae and six other vibrio spp. survival in seawater microcosms as well as uptake of a carbonated substrate in marine or non-marine conditions were investigated. except for v. vulnificus becoming non-culturable, all the other selected species survived in sea water for at least 15 days at 20 degrees c. depending on the species tested, the substrate was better transported in a high salt medium (v. cholerae, v. fluvialis and v. metschnikovii ... | 1994 | 8170408 |
| expression of pna-binding sites on specific glycoproteins by human melanoma cells is associated with a high metastatic potential. | lectin-binding patterns of seven human melanoma clones and variants selected from the same parental cell line and differing in their spontaneous metastatic potential in an animal model were compared by flow cytometry and scatchard analysis. human melanoma clones and variants with high and low metastatic potential could be distinguished by their peanut agglutinin (pna)-binding patterns, but not by their wheat germ agglutinin (wga)-, ulex europaeus agglutinin i (uea i)-, and soybean agglutinin (sb ... | 1994 | 8175891 |
| recombinant derivative of a naturally occurring non-toxinogenic vibrio cholerae 01 expressing the b subunit of cholera toxin: a potential oral vaccine strain. | a clinical isolate of vibrio cholerae 01 was identified which did not possess the heat-labile (ct), the heat-stable (st) or the zonula occludens (zot) toxin genes. rabbit ileal loop assays showed that no other ct-like toxin was produced by this strain. the partly deleted cholera toxin gene which carries the intact gene for the b subunit was cloned and the recombinant plasmid, purd110, was introduced into this non-toxinogenic natural human isolate. the transformed cells (strain urd2) secreted the ... | 1994 | 8178559 |
| structure and arrangement of the cholera toxin genes in vibrio cholerae o139. | the sequence of the ctxb gene encoding the b subunit of cholera toxin has been determined for a strain of vibrio cholerae of the novel o139 serotype associated with recent outbreaks of severe cholera throughout south-east asia and found to be identical to the ctxb gene in v. cholerae o1 of the el tor biotype. analyses by southern hybridization and pcr showed that all strains of the o139 serotype v. cholerae tested carried cholera toxin genes and other genes associated with a virulence cassette d ... | 1994 | 8181723 |
| [mapping a genetic determinant determining the increased synthesis of cholera toxin by the dakka 35 strain of vibrio cholerae]. | a new mutation tox-2 defining the increased level of cholera exotoxin production by the strain vibrio cholerae dakka 35 isolated from nature has been mapped by conjugational crosses of donor and recipient strains differing by toxin production and serovar. the mutation has been localized on the chromosomal fragment containing the ilv, pur, ura, rfb genes adjacent to ura-94 locus. the linkage of the tox-2 mutation with the rfb locus coding for the synthesis of somatic ol-antigen has been also esta ... | 1994 | 8183304 |
| [2 dutch travelers returning from thailand with cholera]. | cholera is a disease rarely imported in the netherlands. recently a 34-year-old woman who had returned from a trip through thailand was admitted to our hospital with complaints of vomiting, watery stools and moderate dehydration. vibrio cholerae oi serotype ogawa biotype el tor was isolated from the faeces. she recovered after antimicrobial and fluid therapy. her 29-year-old travelling companion had only mild symptoms of diarrhoea, but the bacterium was isolated from her stool also. cholera shou ... | 1994 | 8183399 |
| [sepsis caused by non-o1-vibrio cholerae: a patient in the netherlands]. | in a 84-year-old woman extraintestinal infection by non-oi vibrio cholerae was diagnosed. she had septicaemia with cholangitis and cholecysto- and choledocholithiasis. until now 26 patients with non-oi v. cholerae septicaemia have been reported. most had an underlying disease, usually a chronic liver disease or haematological malignancy. these disorders were not present in our patient. she was treated with co-trimoxazole and afterwards she underwent a cholecystectomy and common bile duct explora ... | 1994 | 8183400 |
| sequence determination of rrna genes of pathogenic vibrio species and whole-cell identification of vibrio vulnificus with rrna-targeted oligonucleotide probes. | a comparative analysis of seven new 16s rrna gene sequences of pathogenic vibrio species with previously published vibrio sequences confirmed that vibrio vulnificus represents a group that is not closely related to the core organisms of the genus vibrio. in addition, we found that v. vulnificus, listonella (vibrio) anguillarum and vibrio diazotrophicus branch off separately from the core group. a comparison of the 16s rrna gene sequences of v. vulnificus strains belonging to biotypes 1 and 2 rev ... | 1994 | 8186099 |
| the light organ symbiont vibrio fischeri possesses a homolog of the vibrio cholerae transmembrane transcriptional activator toxr. | a cross-hybridizing dna fragment to vibrio cholerae toxr was cloned from the nonpathogenic light organ symbiont vibrio fischeri, and three proteins homologous to v. cholerae toxr, toxs, and htpg were deduced from its dna sequence. v. fischeri toxr was found to activate a v. cholerae toxr-regulated promoter, and an antiserum raised against the amino-terminal domain of v. cholerae toxr cross-reacts v. fischeri toxr. | 1994 | 8188612 |
| the aquatic environment as a reservoir of vibrio cholerae: a review. | 1993 | 8188990 | |
| personal reflections on the discovery of vibrio cholerae 0139 synonym bengal: a tribute to team work and international collaboration. | 1993 | 8188991 | |
| differential scanning calorimetry investigations on lps and free lipids a of the bacterial cell wall. | differential scanning calorimetry (dsc) was used to investigate the thermal stability and behaviour of the lipopolysaccharides (lps) of the outer membrane of gram-negative bacteria and their lipid portion. dsc curves of lps show thermal features between 200 and 129 degrees c (depolymerization) and between -13 and -36 degrees c (cooling phase transition). both effects were related to the relative strength of the linkage types in the o-chain structure and to their capacity for intermolecular hydro ... | 1993 | 8190999 |
| identification of a novel sugar, 4-amino-4,6-dideoxy-2-o-methylmannose in the lipopolysaccharide of vibrio cholerae o1 serotype ogawa. | a novel sugar in the lipopolysaccharide of vibrio cholerae o1 serotype ogawa has been identified. the sugar was liberated from the lipopolysaccharide when hydrolyzed in 10 m hcl at 90 degrees c for 15 min. the sugar was purified and identified as 4-amino-4,6-dideoxy-2-o-methylmannose (2-o-methylperosamine). since it was found only in the lipopolysaccharide of vibrio cholerae o1 serotype ogawa, it seems that the sugar is one of the specific constituents determining ogawa serotype specificity. | 1994 | 8194067 |
| characterization of the vibrio cholerae outer membrane heme transport protein huta: sequence of the gene, regulation of expression, and homology to the family of tonb-dependent proteins. | the regulation of huta, the vibrio cholerae gene encoding a 77-kda iron-regulated outer membrane protein required for heme iron utilization, was characterized, and the dna sequence of the gene was determined. a huta::tn5 lac fusion generated previously (d. p. henderson and s. m. payne, mol. microbiol. 7:461-469, 1993) was transformed into fur- and fur+ strains of escherichia coli and v. cholerae. the results of beta-galactosidase assays on the transformed strains demonstrated that transcription ... | 1994 | 8195082 |
| rapid detection of vibrio cholerae o1 in stools of peruvian cholera patients by using monoclonal immunodiagnostic kits. loyaza cholera working group in peru. | we compared stool culture with two commercial vibrio cholerae o1 rapid diagnostic kits which detect antigen in 100 adults with cholera in peru. serum vibriocidal-antibody titer was used as an external reference. both rapid diagnostic kits appeared to detect cholera more frequently than did culture and were highly specific. | 1994 | 8195409 |
| non-o1 vibrio cholerae o139 bengal is genetically related to v. cholerae o1 el tor ogawa isolated in mexico. | 1994 | 8195632 | |
| comparative antibacterial spectrum of trimethoprim and brodimoprim. | brodimoprim is a new 2,4-diaminobenzylpyrimidine that selectively inhibits bacterial and resistance plasmid dihydrofolate reductases to a similar or greater extent than trimethoprim. brodimoprim reaches equivalent levels in the serum as trimethoprim for the same dosage regimens but, unlike trimethoprim, brodimoprim has a long half-life. brodimoprim has a similar antibacterial spectrum to trimethoprim against bacterial species normally sensitive to this class of drugs although it is not active ag ... | 1993 | 8195832 |
| [epidemiological, clinical, and microbiological characteristics of the new strain vibrio cholerae 0139]. | 1994 | 8196532 | |
| epidemic cholera in trujillo, peru 1992: utility of a clinical case definition and shift in vibrio cholerae o1 serotype. | epidemic cholera continues in peru. since 1991, cholera surveillance in peru has been based mainly on clinical recognition. to determine the proportion of reported cholera patients who actually have cholera and to evaluate the clinical case definition used in surveillance, we cultured rectal swabs from patients presenting with acute diarrhea in march 1992 in trujillo, peru. of 197 patients meeting the clinical case definition, 174 (88%) had confirmed vibrio cholerae o1 infection. in this epidemi ... | 1994 | 8203704 |
| escherichia coli and vibrio cholerae strains deficient in an enzyme involved in disulphide bond formation (dsba) show an increase in sensitivity to dithiothreitol. | 1994 | 8206309 | |
| gtp-binding proteins associated with the human placental syncytiotrophoblast plasma membrane. | the nature of gtp-binding components associated with isolated human term placental syncytiotrophoblast microvillous plasma membrane vesicles (spmv) was determined; these are relevant to elucidation of intracellular signal transduction mechanisms. four proteins were identified, with molecular weights of 29, 27, 23 and 21 kda, which specifically bound [alpha-32p]gtp in the presence of mg2+. studies employing anti-p21c-ras monoclonal antibodies indicated these four gtp-binding components were ras-r ... | 1994 | 8208666 |
| nonculturable but still viable and potentially pathogenic. | 1993 | 8219487 | |
| analysis of the roles of antilipopolysaccharide and anti-cholera toxin immunoglobulin a (iga) antibodies in protection against vibrio cholerae and cholera toxin by use of monoclonal iga antibodies in vivo. | secretory immunoglobulin a (iga) antibodies (siga) directed against cholera toxin (ct) and surface components of vibrio cholerae are associated with protection against cholera, but the relative importance of specific sigas in protection is unknown. a monoclonal iga directed against the v. cholerae lipopolysaccharide (lps), secreted into the intestines of neonatal mice bearing hybridoma tumors, was previously shown to provide protection against a lethal oral dose of 10(7) v. cholerae cells. we sh ... | 1993 | 8225601 |
| the design and analysis of cholera vaccine trials: recent lessons from bangladesh. | the recent spread of cholera to latin america, together with the persistent burden of this disease in asia and africa, have stimulated efforts to evaluate new cholera vaccines in field settings. although the standard experimental paradigm for vaccine field trials is well established, the success of these trials will also depend on suitable consideration of the epidemiology of cholera and of cholera vaccination in the setting under study. epidemiological studies done in bangladesh emphasize the i ... | 1993 | 8225749 |
| epidemic cholera due to a novel strain of v. cholerae non-01--the beginning of a new pandemic? | 1993 | 8228291 | |
| safety, immunogenicity, and transmissibility of single-dose live oral cholera vaccine strain cvd 103-hgr in 24- to 59-month-old indonesian children. | recombinant a-b+ vibrio cholerae o1 strain cvd 103-hgr is a safe, highly immunogenic, single-dose live oral vaccine in adults in industrialized countries. safety, excretion, immunogenicity, vaccine transmissibility, and environmental introduction of cvd 103-hgr were investigated among 24- to 59-month-old children in jakarta. in 81 households, 1 child was randomly allocated a single dose of vaccine (5 x 10(9) cfu) and another, placebo. additionally, 139 unpaired children were randomly allocated v ... | 1993 | 8228350 |
| the transcriptional activator hlyu of vibrio cholerae: nucleotide sequence and role in virulence gene expression. | hlyu upregulates expression of the haemolysin, hlya, of vibrio cholerae. dna sequence analysis indicates that hlyu is an 11.9 kda protein containing a putative helix-turn-helix motif and belonging to a family of small regulatory proteins, including noir (rhizobium meliloti), smtb (synechococcus pcc 7942) and arsr (plasmids r773, escherichia coli; pi258, staphylococcus aureus; and psx267, staphylococcus xylosus). an hlyu mutant was constructed by insertional inactivation, and found to be deficien ... | 1993 | 8231807 |