Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| subjects with diarrhea-predominant ibs have increased rectal permeability responsive to tryptase. | patients with diarrhea-predominant irritable bowel syndrome (ibs-d) appear to have increased intestinal permeability; it has been suggested that activation of protease-activated receptor-2 (par-2) receptors is responsible for this alteration. the aims of this study are to evaluate (1) if rectal (large bowel) permeability is increased in ibs-d and (2) if tryptase plays a critical role in the altered permeability. | 2010 | 20087660 |
| rapid and reliable detection of 11 food-borne pathogens using thin-film biosensor chips. | traditional methods for identifying food-borne pathogens are time-consuming and laborious, so it is necessary to develop innovative methods for the rapid identification of food-borne pathogens. here, we report the development of silicon-based optical thin-film biosensor chips for sensitive detection of 11 food-borne pathogens. briefly, aldehyde-labeled probes were arrayed and covalently attached to a hydrazine-derivatized chip surface, and then, biotinylated polymerase chain reaction (pcr) ampli ... | 2010 | 20091028 |
| zymographic assay of plant diamine oxidase on entrapped peroxidase polyacrylamide gel electrophoresis. a study of stability to proteolysis. | a zymographic assay of diamine oxidase (dao, histaminase, ec 1.4.3.6), based on a coupled peroxidase reaction, and its behavior at proteolysis in simulated gastric and intestinal conditions, are described. the dao activity from a vegetal extract of lathyrus sativus seedlings was directly determined on sodium dodecyl sulfate polyacrylamide electrophoretic gels containing entrapped horseradish peroxidase, with putrescine as substrate of histaminase and ortho-phenylenediamine as co-substrate of per ... | 2010 | 20091155 |
| [treatment of spinal cord injury by transplanting neural stem cells with ngr gene silencing]. | to determine whether nogo-66 receptor (ngr) gene silencing in neural stem cells (nscs) can ameliorate spinal cord injury (sci) in rats. | 2010 | 20092707 |
| immobilisation of horseradish peroxidase on eupergitc for the enzymatic elimination of phenol. | in this study, three different approaches for the covalent immobilisation of the horseradish peroxidase (hrp) onto epoxy-activated acrylic polymers (eupergitc) were explored for the first time, direct hrp binding to the polymers via their oxirane groups, hrp binding to the polymers via a spacer made from adipic dihydrazide, and hrp binding to hydrazido polymer surfaces through the enzyme carbohydrate moiety previously modified by periodate oxidation. the periodate-mediated covalent immobilisatio ... | 2010 | 20092944 |
| nanoparticle-based sandwich electrochemical immunoassay for carbohydrate antigen 125 with signal enhancement using enzyme-coated nanometer-sized enzyme-doped silica beads. | a novel nanoparticle-based electrochemical immunoassay of carbohydrate antigen 125 (ca125) as a model was designed to couple with a microfluidic strategy using anti-ca125-functionalized magnetic beads as immunosensing probes. to construct the immunoassay, thionine-horseradish peroxidase conjugation (th-hrp) was initially doped into nanosilica particles using the reverse micelle method, and then hrp-labeled anti-ca125 antibodies (hrp-anti-ca125) were bound onto the surface of the synthesized nano ... | 2010 | 20095621 |
| horseradish peroxidase-catalyzed oxidation of chlorophyll a with hydrogen peroxide: characterization of the products and mechanism of the reaction. | horseradish peroxidase was verified to catalyze, without any phenol, the hydrogen peroxide oxidation of chlorophyll a (chl a), solubilized with triton x-100. the 13(2)(s) and 13(2)(r) diastereomers of 13(2)-hydroxychl a were characterized as major oxidation products (ca. 60%) by tlc on sucrose, uv-vis, (1)h, and (13)c nmr spectra, as well as fast-atom bombardment ms. a minor amount of the 15(2)-methyl, 17(3)-phytyl ester of mg-unstable chlorin was identified on the basis of its uv-vis spectrum a ... | 2010 | 20097155 |
| highly sensitive protein detection using enzyme-labeled gold nanoparticle probes. | a highly sensitive protein detection method based on a novel enzyme-labeled gold nanoparticle (aunp) probe has been developed. in this method, we firstly prepared the enzyme-labeled aunp probe by coating aunp with antibody, single-stranded dna (ssdna), and horseradish peroxidase (hrp). magnetic microparticle (mmp) functionalized with another antibody was used as capture probe. then, target protein was sandwiched by the enzyme-labeled aunp probe and the capture probe through immunoreaction. the t ... | 2010 | 20098766 |
| rapid and sensitive dna target detection using enzyme amplified electrochemical detection based on microchip. | a rapid and sensitive dna targets detection using enzyme amplified electrochemical detection (ed) based on microchip was described. we employed a biotin-modified dna, which reacted with avidin-conjugated horseradish peroxidase (avidin-hrp) to obtain the hrp-labeled dna probe and hybridized with its complementary target. after hybridization, the mixture containing dsdna-hrp, excess ssdna-hrp, and remaining avidin-hrp was separated by mce. the separations were performed at a separation voltage of ... | 2010 | 20108259 |
| sequential injection lab-on-valve system for the determination of the activity of peroxidase in vegetables. | horseradish peroxidase (hrp) has been broadly used and investigated for many analytical purposes; it is an enzyme that catalyzes the reduction of hydrogen peroxide in the presence of a reducing compound. the objective of this work was to develop a methodology for the spectrophotometric determination of the activity of peroxidase in vegetable extracts using a flow method with a sequential injection lab-on-valve format. the developed system is based on the reaction between hydrogen peroxide (h(2)o ... | 2010 | 20108899 |
| use of 3-(4-hydroxyphenyl)propionic acid as electron donating compound in a potentiometric aflatoxin m1-immunosensor. | we developed a potentiometric aflatoxin m(1)-immunosensor which utilizes 3-(4-hydroxyphenyl)propionic acid (p-hppa) as electron donating compound for horseradish peroxidase (hrp; ec 1.11.1.7). the assay system consists of a polypyrrole-surface-working electrode coated with a polyclonal anti-m(1) antibody (pab-afm(1)), a ag/agcl reference electrode and a hrp-aflatoxin b(1) conjugate (hrp-afb(1) conjugate). to optimize the potentiometric measuring system p-hppa as well as related compounds serving ... | 2010 | 20113725 |
| multifunctional mesoporous silica nanoparticles as labels for the preparation of ultrasensitive electrochemical immunosensors. | labels based on mesoporous silica nanoparticles (msn) loaded with mediator thionine (th), enzyme horseradish peroxidase (hrp) and secondary anti-human igg antibody (ab(2)) were developed in order to improve the sensitivity and detection limit of an amperometric immunosensor. the sensitivity of the sandwich-type immunosensor using msn-th-hrp-ab(2) as labels for human igg detection was about 100 times higher than that using either msn-th-ab(2) or msn-hrp-ab(2) as labels, indicating the high cataly ... | 2010 | 20116845 |
| enzymatically-crosslinked injectable hydrogels based on biomimetic dextran-hyaluronic acid conjugates for cartilage tissue engineering. | polysaccharide hybrids consisting of hyaluronic acid (ha) grafted with a dextran-tyramine conjugate (dex-ta) were synthesized and investigated as injectable biomimetic hydrogels for cartilage tissue engineering. the design of these hybrids (denoted as ha-g-dex-ta) is based on the molecular structure of proteoglycans present in the extracellular matrix of native cartilage. hydrogels of ha-g-dex-ta were rapidly formed within 2 min via enzymatic crosslinking of the tyramine residues in the presence ... | 2010 | 20116847 |
| glycoprotein clearance is rapid and suppressed by mannan in chicken embryos. | carbohydrates are thought to function as tags that mark circulatory glycoproteins for rapid clearance. scavenger endothelial cells (secs) play the primary role in clearing glycoproteins via receptor-mediated endocytosis in adult animals. we found that horseradish peroxidase (hrp), a glycoprotein, was removed quickly, mostly by receptor mediation from the chicken embryo circulation, but bovine serum albumin was not. the half-life of hrp in the circulation varied with the embryo stage and fell rap ... | 2009 | 20119818 |
| in situ forming hydrogels based on tyramine conjugated 4-arm-ppo-peo via enzymatic oxidative reaction. | over the past decades, hydrogels have been widely studied as biomaterials for various biomedical applications like implants, drugs and cell delivery carriers because of their high biocompatibility, high water contents and excellent permeability for nutrients and metabolites. especially, in situ forming hydrogel systems have received much attention because of their easy application based on minimal invasive techniques. chemical cross-linking systems fabricated using enzymatic reactions have vario ... | 2010 | 20121075 |
| ultrahigh-throughput screening in drop-based microfluidics for directed evolution. | the explosive growth in our knowledge of genomes, proteomes, and metabolomes is driving ever-increasing fundamental understanding of the biochemistry of life, enabling qualitatively new studies of complex biological systems and their evolution. this knowledge also drives modern biotechnologies, such as molecular engineering and synthetic biology, which have enormous potential to address urgent problems, including developing potent new drugs and providing environmentally friendly energy. many of ... | 2010 | 20142500 |
| electrografted poly(n-mercaptoethyl acrylamide) and au nanoparticles-based organic/inorganic film: a platform for the high-performance electrochemical biosensors. | in this study, we describe the use of the combination of eletrografting poly(n-mercaptoethyl acrylamide) and au nanoparticles in the construction of high-performance biosensors. the poly(n-mercaptoethyl acrylamide) was electrografted onto the glassy carbon electrode surface, which provided a strongly adhering primer film for the stable attachment of au nanoparticles and horseradish peroxidase (hrp) enzymes. the performances of the biosensors based on the hrp immobilized in the au/poly(n-mercapto ... | 2010 | 20146284 |
| immunostaining of whole-mount and sectioned lamprey embryos. | lampreys are one of the most basal animals in which many of the true vertebrate characteristics (e.g., neural crest, placodes, segmented brain, skull, paired sensory organs, pharyngeal skeleton) are present. studying the molecular and developmental mechanisms responsible for the formation of these structures in lamprey and higher vertebrates can provide insight into how these vertebrate characteristics evolved. the relative ease of obtaining mature adults and embryos makes this animal an ideal m ... | 2009 | 20147021 |
| evaluation of indirect enzyme-linked immunosorbent assays and igg avidity assays using a protein a-peroxidase conjugate for serological distinction between brucella abortus s19-vaccinated and -infected cows. | this study aimed to evaluate the use of protein a-peroxidase (horseradish peroxidase [hrpo]) in indirect enzyme-linked immunosorbent assays (ielisas) and igg avidity assays for serological distinction between brucella abortus s19-vaccinated and -infected cows. four groups were analyzed: gi, 41 nonvaccinated seropositive cows; gii, 79 s19-vaccinated heifers analyzed at 3 months postvaccination; giii, 105 s19-vaccinated cows analyzed after 24 months of age; and giv, 278 nonvaccinated seronegative ... | 2010 | 20147498 |
| sensitive electrochemical detection of horseradish peroxidase at disposable screen-printed carbon electrode. | a rapid, simple and sensitive electrochemical assay of horseradish peroxidase (hrp) performed on disposable screen-printed carbon electrode was developed. hrp activities were monitored by square-wave voltammetric (swv) measuring the electroactive enzymatic product in the presence of o-aminophenol and hydrogen peroxide substrate solution. swv analysis demonstrated a greater sensitivity and shorter analysis time than the widely used amperometric and differential-pulsed voltammetric methods. the vo ... | 2008 | 20148182 |
| label-free colorimetric assay for methyltransferase activity based on a novel methylation-responsive dnazyme strategy. | dna methylation catalyzed by methyltransferase (mtase) is a significant epigenetic process for modulating gene expression. traditional methods to study mtase activity require a laborious and costly dna labeling process. in this article, we report a simple, colorimetric, and label-free methylation-responsive dnazyme (mr-dnazyme) strategy for mtase activity analysis. this new strategy relies on horseradish peroxidase (hrp) mimicking dnazyme and the methylation-responsive sequence (mrs) of dna whic ... | 2010 | 20148579 |
| stress-induced barrier disruption of rat follicle-associated epithelium involves corticotropin-releasing hormone, acetylcholine, substance p, and mast cells. | the follicle-associated epithelium (fae) is specialized in uptake and sampling of luminal antigens and bacteria. we previously showed that stress increased fae permeability in rats. an increased uptake may alter antigen exposure in peyer's patches leading to intestinal disease. the aim of this study was to elucidate mechanisms involved in the acute stress-induced increase in fae permeability. | 2010 | 20149111 |
| scanning electrochemical microscopy assay of dna based on hairpin probe and enzymatic amplification biosensor. | a novel scheme for scanning electrochemical microscopy (secm) assay of dna based on hairpin probe and enzymatic amplification biosensor was described. in this method, streptavidin-horseradish peroxidase (hrp) was captured by double-stranded dna (ds-dna) modified gold substrate via biotin-streptavidin interaction after hybridization of target dna to the immobilized hairpin probe functioned with a biotin at its 3' end. in the presence of h2o2, hydroquinone (h2q) was oxidized to benzoquinone (bq) a ... | 2010 | 20149629 |
| control of biocatalytic transformations by programmed dna assemblies. | this study demonstrates the self-assembly of inhibitor/enzyme-tethered nucleic acid fragments or enzyme i-, enzyme ii-modified nucleic acids into functional nanostructures that lead to the controlled inhibition of the enzyme or the activation of an enzyme cascade. in one system, the anti-cocaine aptamer subunits are modified with monocarboxy methylene blue (mb(+)) as the inhibitor and with choline oxidase (chox). the cocaine-induced self-assembly of the aptamer subunits complex results in the in ... | 2010 | 20151432 |
| determination of progesterone (p4) from bovine serum samples using a microfluidic immunosensor system. | progesterone (p4) is a steroidal hormone with a vital role in the maintenance of human and animal health. this paper describes the development of an immunosensor coupled to glassy carbon (gc) electrode and integrated to a microfluidic system to quantify p4 from bovine serum samples in a fast and sensitive way. the serum samples spiked with a given p4 concentration and a given p4 concentration bound to horseradish peroxide (hpr) were simultaneously added and, therefore, they competed immunologica ... | 2010 | 20152443 |
| a highly sensitive caffeine immunoassay based on a monoclonal antibody. | a new immunoassay has been developed based on a commercially available anti-caffeine monoclonal antibody and a de novo synthesized tracer, using horseradish peroxidase and uv-visible detection. caffeine, which is frequently found in surface waters, can be quantified with a relative error lower than 20% for concentrations above 0.025 microg l(-1) (limit of quantitation, direct analysis). the limit of detection is 0.001 microg l(-1) and can be reduced by solid-phase extraction (spe). moreover, wit ... | 2010 | 20155491 |
| evolution of gustatory reflex systems in the brainstems of fishes. | the great number of species of teleosts permits highly specialized forms to evolve to occupy particular niches. this diversity allows for extreme variations in brain structure according to particular sensory or motor adaptations. in the case of the taste system, goldfish (carassius auratus l., 1758) and some carps have evolved a specialized intraoral food-sorting apparatus along with corresponding specializations of gustatory centers in the brainstem. a comparison of circuitry within the complex ... | 2009 | 20160963 |
| a microfluidic electrochemical device for high sensitivity biosensing: detection of nanomolar hydrogen peroxide. | we report herein a simple device for rapid biosensing consisting of a single microfluidic channel made from poly(dimethylsiloxane) (pdms) coupled to an injector, and incorporating a biocatalytic sensing electrode, reference and counter electrodes. the sensing electrode was a gold wire coated with 5 nm glutathione-decorated gold nanoparticles (aunps). sensitive detection of h(2)o(2) based on direct bioelectrocatalysis by horseradish peroxidase (hrp) was used for evaluation. hrp was covalently lin ... | 2009 | 20161158 |
| ph-switchable bioelectrocatalysis of hydrogen peroxide on layer-by-layer films assembled by concanavalin a and horseradish peroxidase with electroactive mediator in solution. | the lectin protein concanavalin a (con a) and the glycoenzyme horseradish peroxidase (hrp) were assembled into {con a/hrp}(n) layer-by-layer films on electrodes mainly by biospecific affinity between them. the cyclic voltammetric (cv) response of ferricyanide (fe(cn)(6)(3-)) at {con a/hrp}(n) film electrodes was very sensitive to the environmental ph. the peak currents of fe(cn)(6)(3-) were quite large at ph 4.0 but greatly suppressed at ph 9.0, demonstrating reversible ph-sensitive "on-off" beh ... | 2010 | 20163095 |
| biofunctional titania nanotubes for visible-light-activated photoelectrochemical biosensing. | the photoelectrochemical detection method is a newly developed and promising analytical method for biosensing. in this work, photoactive tio(2) nanotubes (tns) immobilized with horseradish peroxidase (hrp) were prepared and used for visible-light-activated photoelectrochemical detection of h(2)o(2). tns were fabricated by anodic oxidation of titanium substrate and possessed large surface areas, good uniformity and conformability, and high porosity, which were favorable for enzyme immobilization. ... | 2010 | 20163104 |
| histochemical application of a peroxidase dnazyme with a covalently attached hemin cofactor. | the enzyme horseradish peroxidase is routinely used in immunohistochemistry to facilitate the chromogenic oxidation of 3,3'-diaminobenzidine, producing an insoluble brown precipitate marking the location and quantity of a tissue protein. in an effort to develop non-protein reagents for tissue diagnostics, we have developed a peroxidase dnazyme construct that can be used as a chromogenic functional group in immunohistochemistry assays. the dnazyme is based on a reported 18-mer g-quadruplex-formin ... | 2010 | 20166673 |
| supramolecular hydrogels exhibiting fast in situ gel forming and adjustable degradation properties. | fast in situ forming supramolecular hydrogels consisted of the tyramine-conjugated supramolecular structures and chitosan derivative were prepared via an enzymatic reaction with horseradish peroxidase (hrp) and hydrogen peroxide (h(2)o(2)). the gel formation was varied within a time period of 5 s to 10 min by controlling the concentrations of hrp, h(2)o(2), and polymers. tyramine conjugation at different sites of the supramolecular structure resulted in significant changes in physical properties ... | 2010 | 20166679 |
| receptor-mediated and fluid-phase transcytosis of horseradish peroxidase across rat hepatocytes. | horseradish peroxidase (hrp) is often used as a fluid-phase marker to characterize endocytic and transcytotic processes. likewise, it has been applied to investigate the mechanisms of biliary secretion of fluid in rat liver hepatocytes. however, hrp contains mannose residues and thus binds to mannose receptors (mrs) on liver cells, including hepatocytes. to study the role of mr-mediated endocytosis of hrp transport in hepatocytes, we determined the influence of the oligosaccharid mannan on hrp b ... | 2010 | 20168981 |
| immunochemical determination of oxytetracycline in fish: comparison between enzymatic and time-resolved fluorometric assays. | an indirect competitive enzyme-linked immunosorbent assay (elisa) with photometric detection of horseradish peroxidase (hrp) activity, was developed in plate to detect oxytetracycline (otc) in gilthead sea bream (sparus aurata) samples. the results were compared to those obtained by time-resolved fluoroimmunoassay (tr-fia) using a secondary antibody with coproporphyrin of platinum (ii) (ptcp) as marker. the limits of detection obtained in fish extract were 16 and 0.08 microg kg(-1) for photometr ... | 2010 | 20171317 |
| self-assembled, photoluminescent peptide hydrogel as a versatile platform for enzyme-based optical biosensors. | a self-assembled peptide hydrogel consisting of fmoc-diphenylalanine has been employed as a biosensing platform through the encapsulation of enzyme bioreceptors (e.g., glucose oxidase or horseradish peroxidase) and fluorescent reporters (e.g., cdte and cdse quantum dots). enzymes and quantum dots (qds) were physically immobilized within the hydrogel matrix in situ in a single step by simply mixing aqueous solution containing qds and enzymes with monomeric peptide (fmoc-diphenylalanine) solution. ... | 2011 | 20171868 |
| electrochemical behavior of biocatalytical composite based on heme-proteins, didodecyldimethylammonium bromide and room-temperature ionic liquid. | a novel biocompatible composite film based on a water-insoluble surfactant, didodecyldimethylammonium bromide (ddab), and a hydrophobic room-temperature ionic liquid (rtil), 1-hexyl-3-methyl-imidazolium hexafluorophosphate (himimpf(6)), for the immobilization of biocatalytical proteins was reported. differential scanning calorimetry (dsc) showed that the ddab-himimpf(6) composite film has higher thermal stability than the ddab film alone. sem images indicated that different microstructures exist ... | 2010 | 20172091 |
| altered renal proximal tubular endocytosis and histology in mice lacking myosin-vi. | myosin vi (myo6) is an actin-based molecular motor involved in clathrin-mediated endocytosis that is highly expressed in the renal proximal tubule brush border. we investigated the renal physiological consequences of loss of myo6 function by performing renal clearance and physiological measurements on myo6 functional null snell's waltzer (sv/sv) and control heterozygous (+/sv) mice. sv/sv mice showed reduced body weight and elevated blood pressure compared with controls; no differences were obse ... | 2010 | 20175219 |
| advantages of soybean peroxidase over horseradish peroxidase as the enzyme label in chemiluminescent enzyme-linked immunosorbent assay of sulfamethoxypyridazine. | an indirect competitive chemiluminescent enzyme-linked immunosorbent assay (cl-elisa) of sulfamethoxypyridazine (smp) was developed. the conjugates of streptavidin with cationic horseradish peroxidase (hrp) and anionic soybean peroxidase (sbp) were used in cl-elisa for the detection of biotinylated anti-smp antibodies. for streptavidin-hrp conjugate-catalyzed chemiluminescence measured 20 s after the initiation of the enhanced chemiluminescence reaction (ecr), the limit of detection (ic(10)), th ... | 2010 | 20175541 |
| controlled assembly for well-defined 3d bioarchitecture using two active enzymes. | this paper reports that a bioarchitecture with two different active enzymes can be fabricated conveniently on a prepatterned surface by highly selective surface-templated layer-by-layer (lbl) assembly by coupling a bilayer of avidin/biotin-lactate oxidase (biotin-lod) with a bilayer of avidin/biotin-horseradish peroxidase (biotin-hrp). the two different active enzymes can be utilized as excellent building blocks for the fabrication of well-defined 3d nanostructures with precise control of the po ... | 2010 | 20180558 |
| mast cells in squamous cell esophageal carcinoma and clinical parameters. | esophageal carcinoma is a malignancy with a poor prognosis and new treatment modalities must be sought. one possibility that has been tested in patients with malignant melanoma is treatment which aims towards boosting the immune system. in the present study, we investigated the role of mast cells in patients with esophageal carcinoma. the intention was to determine whether a higher number of mast cells is associated with better survival and may thus be a marker for future immunotherapeutic studi ... | 2015 | 20181628 |
| peroxide decoloration of ci acid orange 7 catalyzed by manganese chlorophyll derivatives at the surfaces of micelles and lipid bilayers. | manganese-substituted chlorophyll a derivatives (mnchls) were synthesized. we first report peroxidative oxidation of an azo dye, ci acid orange 7, catalyzed by mnchls at the surfaces of micelles and lipid bilayers with hydrogen peroxide (h(2)o(2)) under mild conditions (ph 8.0, 25 degrees c). peroxide decoloration depended upon the structures of mnchls, surfactants, lipids, and the presence of imidazole. surprisingly, a largest decoloration rate was observed for mnchls dimer, mnpchlide a-k(mnpch ... | 2010 | 20184352 |
| a comparison of the postnatal development of muscle-spindle and periodontal-ligament neurons in the mesencephalic trigeminal nucleus of the rat. | the trigeminal mesencephalic nucleus (vmes) is known to include primary afferent neurons of jaw muscle spindles (ms neurons) and periodontal ligament receptors (pl neurons). the aim of this study was to clarify the postnatal development of vmes neurons by comparing ms neurons with pl neurons using horseradish peroxidase labeling. we measured somal diameter and somal shape of ms and pl neurons in rats from postnatal day (p)7 to p70. no significant changes were seen between postnatal day p7 and p7 ... | 2010 | 20184942 |
| a novel enzyme biosensor for glucose based on rhodanine derivative chemiluminescence system and mesoporous hollow silica microspheres receptor. | in this work, hollow silica microspheres (hsms), which have hollow interiors and nanoporous shells, were used in the immobilization of enzyme to establish a novel chemiluminescence biosensor. the immobilization behavior of enzyme in hsms with different pore sizes has been studied. the results revealed that the pore size and the surface area of hsms play important roles in their immobilization performance. compared with traditional methods, this immobilization method not only provides tunable and ... | 2010 | 20185291 |
| [experimental study on bone marrow mesenchymal stem cells seeded in chitosan-alginate scaffolds for repairing spinal cord injury]. | to investigate tissue engineered spinal cord which was constructed of bone marrow mesenchymal stem cells (bmscs) seeded on the chitosan-alginate scaffolds bridging the both stumps of hemi-transection spinal cord injury (sci) in rats to repair the acute sci. | 2010 | 20187451 |
| application of the amplex red/horseradish peroxidase assay to measure hydrogen peroxide generation by recombinant microsomal enzymes. | the formation of reactive oxygen species by the cytochrome p450 monooxygenase system is thought to be due to autoxidation of nadph-cytochrome p450 reductase and the nonproductive decay of oxygen-bound cytochrome p450 intermediates. to characterize this process in recombinant microsomal enzymes, we used a highly sensitive hydrogen peroxide assay based on amplex red oxidation. this assay is 20 times more sensitive (lld=5.0pmol/assay and llq=30pmol/assay) than the standard ferrous thiocyanate assay ... | 2010 | 20188819 |
| ultrasensitive electrochemical immunosensor for oral cancer biomarker il-6 using carbon nanotube forest electrodes and multilabel amplification. | squamous cell carcinomas of head and neck (hnscc) are associated with immune, inflammatory, and angiogenic responses involving interleukin-6 (il-6). this article reports an ultrasensitive electrochemical immunosensor for human il-6 and proof-of-concept studies of il-6 detection in hnscc cells. single wall carbon nanotube (swnt) forests with attached capture antibodies (ab(1)) for il-6 were used in an electrochemical sandwich immunoassay protocol using enzyme label horseradish peroxidase (hrp) to ... | 2010 | 20192182 |
| [correlation of toll-like receptor 3 and tumor necrosis factor-alpha with idiopathic fetal growth restriction.]. | to investigate the expression and the significance of toll-like receptor 3 (tlr-3) in placenta, tumor necrosis factor-alpha(tnf-alpha) in maternal and cord blood of idiopathic fetal growth restriction (ifgr), and their correlation with the pathogenesis of symmetric and asymmetric ifgr. | 2009 | 20193417 |
| [luminol oxidation by hydrogen peroxide with chemiluminescent signal formation catalyzed by peroxygenase from the fungus agrocybe aegerita v.brig]. | conditions of luminol oxidation by hydrogen peroxide in the presence of peroxygenase from the mushroom agrocybe aegerita v.brig have been optimized. the ph value (8.8) at which fungal peroxygenase produces a maximum chemiluminescent signal has been shown to be similar to the ph optimum value of horseradish peroxidase. luminescence intensity changed when the concentration of tris buffer was varied; maximum intensity of chemiluminescence was observed in 40 mm solution. it has been shown that enhan ... | 2013 | 20198921 |
| decontamination of chemical and biological warfare agents with a single multi-functional material. | we report the synthesis of new polymers based on a dimethylacrylamide-methacrylate (dmaa-ma) co-polymer backbone that support both chemical and biological agent decontamination. polyurethanes containing the redox enzymes glucose oxidase and horseradish peroxidase can convert halide ions into active halogens and exert striking bactericidal activity against gram positive and gram negative bacteria. new materials combining those biopolymers with a family of n-alkyl 4-pyridinium aldoxime (4-pam) hal ... | 2010 | 20199807 |
| sensitive immunosensor for cancer biomarker based on dual signal amplification strategy of graphene sheets and multienzyme functionalized carbon nanospheres. | a novel electrochemical immunosensor for sensitive detection of cancer biomarker alpha-fetoprotein (afp) is described that uses a graphene sheet sensor platform and functionalized carbon nanospheres (cnss) labeled with horseradish peroxidase-secondary antibodies (hrp-ab2). greatly enhanced sensitivity for the cancer biomarker is based on a dual signal amplification strategy: first, the synthesized cnss yielded a homogeneous and narrow size distribution, which allowed several binding events of hr ... | 2010 | 20201502 |
| membrane targeted horseradish peroxidase as a marker for correlative fluorescence and electron microscopy studies. | synaptic dynamics and reorganization are fundamental features of synaptic plasticity both during synaptic circuit development and in the mature cns underlying learning, memory, and experience-dependent circuit rearrangements. combining in vivo time-lapse fluorescence imaging and retrospective electron microscopic analysis provides a powerful technique to decipher the rules governing dynamics of neuronal structure and synaptic connections. here we have generated a membrane-targeted horseradish pe ... | 2010 | 20204144 |
| divergent development of testosterone secretion in male zebu (bos indicus) and crossbred cattle (bos indicus x bos taurus) and buffaloes (bubalus bubalis) during growth. | delayed pubertal development and low fertility of bos indicus x bos taurus crossbred male cattle and domestic buffaloes is hardly understood hence, a sensitive enzymeimmunoassay (eia) was developed using the second antibody-coating technique and testosterone-3-o-carboxymethyloxime-horseradish peroxidase conjugate as a label for determination of testosterone in blood plasma. the eia was validated by standard criteria. blood samples were collected by venipuncture from growing male cattle (karan fr ... | 2010 | 20213223 |
| enzymatically crosslinked dextran-tyramine hydrogels as injectable scaffolds for cartilage tissue engineering. | enzymatic crosslinking of dextran-tyramine (dex-ta) conjugates in the presence of horseradish peroxidase and hydrogen peroxide was successively applied in the preparation of hydrogels. depending on the molecular weight of the dextran (m(n,) (gpc) of 14000 or 31000 g/mol) and the degree of substitution (of 5, 10, or 15) with ta groups, the gelation times ranged from 20 s to 1 min. hydrogels prepared from dex31k-ta with a degree of substitution of 10 had storage moduli up to 60 kpa. similar values ... | 2010 | 20214454 |
| sustained synaptic-vesicle recycling by bulk endocytosis contributes to the maintenance of high-rate neurotransmitter release stimulated by glycerotoxin. | glycerotoxin (gltx), a large neurotoxin isolated from the venom of the sea worm glycera convoluta, promotes a long-lasting increase in spontaneous neurotransmitter release at the peripheral and central synapses by selective activation of ca(v)2.2 channels. we found that gltx stimulates the very high frequency, long-lasting (more than 10 hours) spontaneous release of acetylcholine by promoting nerve terminal ca(2+) oscillations sensitive to the inhibitor omega-conotoxin gvia at the amphibian neur ... | 2010 | 20215402 |
| stabilization and release of enzymes from silk films. | a significant challenge remains to protect protein drugs from inactivation during production, storage, and use. in the present study, the stabilization and release of horseradish peroxidase (hrp) in silk films was investigated. water-insoluble silk films were prepared under mild aqueous conditions, maintaining the activity of the entrapped enzyme. depending on film processing and post-processing conditions, hrp retained more than 90% of the initial activity at 4 degrees c, room temperature and 3 ... | 2010 | 20217856 |
| indole-3-acetic acid/horseradish peroxidase induces apoptosis in tccsup human urinary bladder carcinoma cells. | indole-3-acetic acid (iaa) and horseradish peroxidase (hrp) have emerged as a new strategy for cancer treatment. in the present study, we determined the effects of iaa/hrp treatment on tccsup human urinary bladder carcinoma cells. it was found that the iaa/hrp combination decreased cell viability of tccsup cells in a time- and dose-dependent manner, whereas iaa or hrp alone showed no such effect. in addition, the decreased cell viability was restored by pretreatment with ascorbic acid. to clarif ... | 2010 | 20225657 |
| novel thermosensitive calcium alginate microspheres: physico-chemical characterization and delivery properties. | the system described in this paper was obtained by soaking calcium alginate (caalg) microspheres in a water solution of poly-[(3-acrylamidopropyl)-trimethylammonium chloride-b-n-isopropylacrylamide] [poly(amptma-b-nipaam)], a new block co-polymer recently synthesized by atom transfer radical polymerization (atrp). the block co-polymer is characterized by a lower critical solution temperature (lcst) of 41 degrees c in aqueous 0.1 m nacl solution, and can be anchored on the caalg microspheres by m ... | 2010 | 20226887 |
| oxidation of 2-t-butyl-4-methoxyphenol (bha) by horseradish and mammalian peroxidase systems. | di-bha, 2,2'-dihydroxy-3,3'-di-t-butyl-5,5'-dimethoxy-diphenyl, was isolated as the product of the reaction of either commercial horseradish peroxidase or partially purified rat intestine peroxidase (donor-h(2)o(2) oxidoreductase, ec 1.11.1.7.) and hydrogen peroxide with 2-t-butyl-4-methoxyphenol (bha). bha, di-bha and other cyclic compounds possessing a hydroxyl group in the ring were found to be competitive inhibitors with respect to guaiacol, and non-competitive inhibitors with respect to hyd ... | 1980 | 20227953 |
| synaptic organization of substance p, glutamate and gaba-immunoreactive boutons on functionally identified neurons in cat spinal deeper dorsal horn. | in order to determine how nociceptive input conveyed by the c-fibers terminating in superficial laminae of the spinal cord reaches the wide dynamic range (wdr) cells in deeper dorsal horn, which functions as ascending projection pathway, the morphological features of some wdr cells in the deeper dorsal horn of the cat lumbar spinal cord were studied by intracellular injection of horseradish peroxidase and physiological characterization. one of the fully stained neurons with somata in lamina v an ... | 1997 | 20229302 |
| surfactant-stabilized small hydrogel particles in oil: hosts for remarkable activation of enzymes in organic solvents. | hydrogels of amino acid based cationic surfactant having c(16) tails were used to immobilize heme proteins and enzyme. these hydrogel-entrapped proteins/enzyme showed remarkable activation when dispersed in organic solvent. the activation effect (ratio of the activity of the hydrogel-entrapped enzyme in organic solvent to the activity of the native enzyme in water) of cytochrome c increased up to 350-fold with varying protein and gelator concentration. hydrogel-entrapped hemoglobin and horseradi ... | 2010 | 20229535 |
| enzyme-linked enzyme-binding assay for pin1 ww domain ligands. | peptidyl prolyl cis-trans isomerase (ppiase) interacting with nima-1 (pin1) catalyzes the cis-trans isomerization of pser/pthr-pro amide bonds. pin1 is a two-domain protein that represents a promising target for the treatment of cancer. both domains of pin1 bind the pser/pthr-pro motif; ppiase enzymatic activity occurs in the catalytic domain, and the ww domain acts as a recognition module for the pser/pthr-pro motif. an assay we call an enzyme-linked enzyme-binding assay (eleba) was developed t ... | 2010 | 20230769 |
| sno2@poly(hema-co-st-co-vpba) core-shell nanoparticles designed for selectively enriching glycopeptides followed by maldi-ms analysis. | the core-shell boronic-acid functionalized nanoparticles sno(2)@poly(hema-co-st-co-vpba) are designed for selectively enriching glycopeptides, followed by matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms) analysis. such 60 nm sized core-shell nanoparticles are prepared by means of copolymerization between 2-hydroxyethyl methacrylate (hema) grafted on sno(2) nanoparticles, styrene, and 4-vinylphenylboronic acid (vpba). all of the synthesis procedures are completed within 3 ... | 2010 | 20235269 |
| [not available]. | 1947 | 20254343 | |
| directing the oligomer size distribution of peroxidase-mediated cross-linked bovine alpha-lactalbumin. | enzymatic protein cross-linking is a powerful tool to change protein functionality. for optimal functionality in gel formation, the size of the cross-linked proteins needs to be controlled, prior to heating. in the current study, we addressed the optimization of the horseradish peroxidase-mediated cross-linking of calcium-depleted bovine alpha-lactalbumin. to characterize the formed products, the molecular weight distribution of the cross-linked protein was determined by size exclusion chromatog ... | 2010 | 20297813 |
| integrated multienzyme electrochemical biosensors for monitoring malolactic fermentation in wines. | integrated amperometric biosensors for the determination of l-malic and l-lactic acids were developed by coimmobilization of the enzymes l-malate dehydrogenase (mdh) and diaphorase (dp), or l-lactate oxidase (lox) and horseradish peroxidase (hrp), respectively, together with the redox mediator tetrathiafulvalene (ttf), on a 3-mercaptopropionic acid (mpa) self-assembled monolayer (sam)-modified gold electrode by using a dialysis membrane. the electrochemical oxidation of ttf at +100mv (vs. ag/agc ... | 2010 | 20298874 |
| a fast and sensitive enzyme immunoassay for brain natriuretic peptide based on micro-magnetic probes strategy. | in this paper, a simple, rapid and low-cost method for the high-sensitivity detection of brain natriuretic peptide (bnp) was developed, which adopted three amplification steps: (a) biotin-streptavidin amplification; (b) micro-magnetic probe amplification; (c) hrp (horseradish peroxidase) signal amplification. in the present strategy, the streptavidin-coated micro-magnetic particles (mmps) were first conjugated with biotin-labeled capture antibody via the biotin-streptavidin interaction, which fo ... | 2010 | 20298887 |
| the high-adhesive properties of the fimh adhesin of salmonella enterica serovar enteritidis are determined by a single f118s substitution. | the binding properties of low- and high-adhesive forms of fimh adhesins from salmonella enterica serovars enteritidis and typhimurium (s. enteritidis and s. typhimurium) were studied using chimeric proteins containing an additional peptide that represents an n-terminal extension of the fimf protein. this modification, by taking advantage of a donor strand exchange mechanism, closes the hydrophobic groove in the fimbrial domain of the fimh adhesin. such self-complemented adhesins (scfimh) did not ... | 2010 | 20299404 |
| pro-oxidant activity of apocynin radical. | apocynin has been widely used as an nadph oxidase inhibitor in many experimental models. however, concern regarding the efficacy, selectivity, and oxidative side effects of the inhibitor is increasing. in this study, our aim was to characterize the pro-oxidant properties of apocynin and the structurally-related compounds vanillin and vanillic acid. glutathione (gsh), cysteine, ovalbumin, and the coenzyme nadph were chosen as potential target biomolecules that could be affected by transient free ... | 2010 | 20304045 |
| kaempferol complexation in cyclodextrins at basic ph. | the complexation of kaempferol with cyclodextrins (cds) (beta-, g(2)-beta-, and hp-beta-cds) in basic medium was studied, and the complexation constants (k(c)) were calculated by using enzymatic, solubility, and fluorometric methods. this is the first time that a decrease in fluorescence has been observed as result of the analyte complexation by cds. the highest k(c) value for kaempferol complexation was obtained for hp-beta-cds. to establish the validity of the fluorometric method for determini ... | 2010 | 20329774 |
| high-throughput single-base mismatch detection for genotyping of udp-glucuronosyltransferase (ugt1a1) with probe capture assay coupled with modified allele-specific primer extension reaction (masper). | we have developed a new method based on specific primer extension reactions coupled with plate hybridization for high-throughput genotyping of single-base mutations. to improve the switching characteristics of the primer extension reaction, we introduced an artificial mismatch two bases upstream of the 3'-terminal base in the detection primers. a set of primers that correspond to wild-type and mutant dna segments can be used to accurately analyze single-base mutations. the termini of these prime ... | 2010 | 20333765 |
| trace-level detection of atrazine using immuno-chemiluminescence: dipstick and automated flow injection analyses formats. | a sensitive chemiluminescence (cl)-based immunoassay technique based on both dipstick and flow injection analytical formats is reported for the detection of atrazine. in the dipstick-based immunoassay technique, antibody (anti-atrazine) was first immobilized on the nitrocellulose membranes. the dipstick was then treated with atrazine and atrazine-horseradish peroxidase conjugate (atra-hrp) to facilitate the competitive binding. the dipstick was further treated with urea-hydrogen peroxide (u-h202 ... | 2013 | 20334163 |
| analysis of ddt isomers with enzyme-linked immunosorbent assay and optical immunosensor based on rat monoclonal antibodies as biological recognition elements. | new rat monoclonal antibodies (mabs) for ddt [1,1,1 -trichloro-2,2-bis (4-chlorophenyl) ethane], namely ddt 7c12, ddt 1c1, and ddt 1b2, were developed, characterized, and applied in elisa both in coating antigen and in enzyme-tracer format. the latter used horseradish peroxidase (hrp) or glucose oxidase as enzymes. the lowest concentration of p,p'-ddt was determined with mab ddt 7c12 and ddt-hapten hrp, with a test midpoint (ic50) of 0.5 +/- 0.2 microg/l (n=10) in 40 mm pbs (phosphate-buffered s ... | 2010 | 20334165 |
| electrocatalytic microelectrode detectors for choline and acetylcholine following separation by capillary electrophoresis. | two electrocatalytic enzyme modified microelectrode systems were employed as end-column amperometric detectors of choline (ch) and acetylcholine (ach) following separation by capillary electrophoresis (ce). horseradish peroxidase cross-linked in an osmium based redox polymer hydrogel (hrp-os) was physically adsorbed on au microelectrodes followed by chemical cross-linking of the enzymes acetylcholinesterase (ache) and choline oxidase (cho). an alternative approach utilized the deposition of the ... | 2009 | 20337384 |
| cona and uea-i lectin histochemistry of parotid gland mucoepidermoid carcinoma. | mucoepidermoid carcinoma (mec) corresponds to 5-12% of all salivary gland tumours, and is classified as low, intermediate or high grade. traditionally, immunohistochemistry was considered as the complementary tool for diagnosis of salivary gland neoplasia. lectin histochemistry has also been increasingly used in recent years. in this work, lectins were used as histochemical markers for normal and transformed parotid glands. biopsy specimens of 15 cases diagnosed as mec (low, intermediate and hig ... | 2010 | 20339232 |
| are the thalamic projections of nucleus z of the medulla oblongata reorganized after partial deafferentation of the ventrolateral nucleus of the thalamus? | the possible plastic reorganization of projections from the somatosensory relay nucleus z of the cat medulla oblongata to the partially deafferented ventrolateral nucleus of the thalamus was studied by retrograde labeling with horseradish peroxidase. partial deafferentation of the ventrolateral nucleus of the thalamus was produced by prior (three months) electrolytic destruction of the contralateral cerebellar interpositus nucleus or the lateral vestibular nucleus of deiters. the results demonst ... | 2010 | 20339945 |
| on the role of water in peroxidase catalysis: a theoretical investigation of hrp compound i formation. | we have investigated the dynamics of water molecules in the distal pocket of horseradish peroxidase to elucidate the role that they may play in the formation of the principal active species of the enzymatic cycle (compound i, por(o+)-fe(iv)=o) upon reaction of the resting fe(iii) state with hydrogen peroxide. the equilibrium molecular dynamics simulations show that, in accord with experimental evidence, the active site access channel is hydrated with an average of two to three water molecules wi ... | 2010 | 20345187 |
| gamma' fibrinogen: evaluation of a new assay for study of associations with cardiovascular disease. | studies of disease associations with gamma' fibrinogen, a newly emerging risk factor for cardiovascular disease, have been hampered by the lack of a standardized and well-characterized assay. | 2010 | 20348406 |
| determination of beta-glucosidase activity in soils with a bioanalytical sensor modified with multiwalled carbon nanotubes. | soil microorganisms and enzymes are the primary mediators of soil biological processes, including organic matter degradation, mineralization, and nutrient recycling. they play an important role in maintaining soil ecosystem quality and functional diversity. moreover, enzyme activities can provide an indication of quantitative changes in soil organic matter. beta-glucosidase (beta-glu) activity has been found to be sensitive to soil management and has been proposed as a soil quality indicator bec ... | 2010 | 20349226 |
| enzyme-based multiplexer and demultiplexer. | a digital 2-to-1 multiplexer and a 1-to-2 demultiplexer were mimicked by biocatalytic reactions involving concerted operation of several enzymes. using glucose oxidase (gox) and laccase (lac) as the data input signals and variable ph as the addressing signal, ferrocyanide oxidation in the output channel was selectively activated by one from two inputs, thus mimicking the multiplexer operation. a demultiplexer based on the enzyme system composed of gox, glucose dehydrogenase (gdh) and horseradish ... | 2010 | 20350002 |
| virgin olive oil administration improves the effect of aspirin on retinal vascular pattern in experimental diabetes mellitus. | the aim of the present study is to evaluate the possible influence of virgin olive oil (voo) on the effect of acetylsalicylic acid (asa) in platelet aggregation, prostanoid and no production and retinal vascular pattern in rats with experimental type 1-like diabetes. we used 100 male wistar rats that were distributed into five groups: (1) non-diabetic rats (ndr); (2) untreated diabetic rats (dr); (3) dr treated with asa (2 mg/kg per d per os (p.o.)); (4) dr treated with voo (0.5 ml/kg per d p.o. ... | 2010 | 20350343 |
| [investigation of micro-aqueous covalent immobilization of horseradish peroxidase by "conformation memory"]. | we has studied the feasibility of preventing protein from denature during covalent immobilization by "conformation memory", which was achieved by freeze-drying under enzyme active conformation and cross-linked with carrier under micro-aqueous media (mam). horseradish peroxidase (hrp) and chitosan beads have been used as the model enzyme and carrier. the mam consisted of 99% dioxane and 1% water. we compared the immobilized hrp under mam with that under traditional aqueous solvent, found that the ... | 2009 | 20352976 |
| [effects of aqueous-organic solvents on peroxidase mimetic activity of fe3o4 magnetic nanoparticles]. | we prepared fe3o4 magnetic nanoparticles (mnps) with the size of 10 nm by chemical coprecipitation. the effects of six aqueous-organic solvents, including tetrahydrofuran, dioxane, acetone, n, n-dimethylformamide, methylalcohol, and dimethyl sulfoxide, on peroxidase mimetic activity of fe3o4 mnps were studied and compared with that of horseradish peroxidase (hrp). the relative activity of fe3o4 mnps droped sharply as the elevation of organic solvent concentration increased from 30% to 75% (v/v). ... | 2009 | 20352977 |
| quantitative enzyme activity determination with zeptomole sensitivity by microfluidic gradient-gel zymography. | we describe a sensitive zymography technique that utilizes an automated microfluidic platform to report enzyme molecular weight, amount, and activity (including k(cat) and k(m)) from dilute protein mixtures. calf intestinal alkaline phosphatase (cip) is examined in detail as a model enzyme system, and the method is also demonstrated for horseradish peroxidase (hrp). the 40 min assay has a detection limit of 5 zmol ( approximately 3 000 molecules) of cip. two-step pore-limit electrophoresis with ... | 2010 | 20353191 |
| nanogold-enwrapped graphene nanocomposites as trace labels for sensitivity enhancement of electrochemical immunosensors in clinical immunoassays: carcinoembryonic antigen as a model. | a new, highly sensitive electrochemical immunosensor with a sandwich-type immunoassay format was designed to quantify carcinoembryonic antigen (cea), as a model tumor marker, using nanogold-enwrapped graphene nanocomposites (nggns) as trace labels in clinical immunoassays. the device consisted of a glassy carbon electrode coated with prussian blue (pb) on whose surface gold nanoparticles were electrochemically deposited to the further modified with the specific analyte-capturing molecule, anti-c ... | 2010 | 20353889 |
| oscillating chemiluminescence systems: state of the art. | oscillating chemiluminescence (cl) was reported for the first time about 30 years ago. since then several systems based on addition of a chemiluminescent reagent to a known oscillator system or based on the light emitting features of one component of the oscillating system, have been described. this information, scattered in the scientific literature, is compiled in the present paper. several oscillating cl systems, including those based on belousov-zhabotinskii and orban oscillators, or horsera ... | 2011 | 20354969 |
| direct competitive enzyme-linked immunosorbent assay (elisa) for rapid screening of anisakid larvae in seafood. | anisakid larvae are one of the most important pathogenic parasites in marine products; however, simple and rapid analytical techniques for them are still very limited. in this research, based on specific rabbit polyclonal antibodies which were raised against crude extracts of anisakis larvae, purified by protein a affinity chromatography and labeled with horseradish peroxidase, a direct competitive enzyme-linked immunosorbent assay (elisa) was developed and validated for detection of anisakid la ... | 2010 | 20355125 |
| sensitive enzyme-linked immunosorbent assay and rapid one-step immunochromatographic strip for fumonisin b1 in grain-based food and feed samples. | maize contaminated with mycotoxin fumonisin b1 poses a global threat to agricultural production. in this study, polyclonal antibodies (pab) specific to fumonisin b1 were generated from rabbits immunised with fumonisin b1-keyhole limpet haemocyanin (klh). these antibodies were used to establish a sensitive competitive direct enzyme-linked immunosorbent assay (cdelisa) and gold nanoparticle immunochromatographic strip for detecting fumonisin b1 levels in maize-based foods and feeds. | 2010 | 20355142 |
| direct electrochemistry of horseradish peroxidase immobilized on the layered calcium carbonate-gold nanoparticles inorganic hybrid composite. | a mediator-free hydrogen peroxide (h(2)o(2)) biosensor was fabricated based on immobilization of horseradish peroxidase (hrp) on layered calcium carbonate-gold nanoparticles (caco(3)-aunps) inorganic hybrid composite. the proposed biosensor showed a strong electrocatalytic activity toward the reduction of h(2)o(2), which could be attributed to the favored orientation of hrp in the well-confined surface as well as the high electrical conductivity of the resulting caco(3)-aunps inorganic hybrid co ... | 2010 | 20356730 |
| sensitive electrochemical immunosensor for matrix metalloproteinase-3 based on single-wall carbon nanotubes. | a novel electrochemical immunosensor for the detection of matrix metalloproteinase-3 (mmp-3), a cancer biomarker protein, based on vertically aligned single-wall carbon nanotube (swcnt) arrays is presented. detection was based on a sandwich immunoassay consisting of horseradish peroxidase (14-16 labels) conjugated to a secondary antibody and/or a polymer bead loaded with multi-enzyme labels. performance was optimized by effective minimization of non-specific binding (nsb) events using bovine ser ... | 2010 | 20358056 |
| lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function. | characterization of leukocytes is an integral part of blood analysis and blood-based diagnostics. in the present paper, we combine lensless holographic imaging with antibody microarrays for rapid and multiparametric analysis of leukocytes from human blood. monoclonal antibodies (abs) specific for leukocyte surface antigens (cd4 and cd8) and cytokines (tnf-alpha, ifn-gamma, il-2) were printed in an array so as to juxtapose cell capture and cytokine detection antibody (ab) spots. integration of ab ... | 2010 | 20359168 |
| carbon nanotubes degraded by neutrophil myeloperoxidase induce less pulmonary inflammation. | we have shown previously that single-walled carbon nanotubes can be catalytically biodegraded over several weeks by the plant-derived enzyme, horseradish peroxidase. however, whether peroxidase intermediates generated inside human cells or biofluids are involved in the biodegradation of carbon nanotubes has not been explored. here, we show that hypochlorite and reactive radical intermediates of the human neutrophil enzyme myeloperoxidase catalyse the biodegradation of single-walled carbon nanotu ... | 2010 | 20364135 |
| [stabilization of peroxidase conjugates used in enzyme immunoassay systems to detect ebola and marburg virus antigens]. | the time course of changes in the activity of solutions of horseradish peroxidase conjugates with immunoglobulins against ebola and marburg fevers was studied in the presence of different components. the series of the conjugates of elisa kits for the detection of ebola and marburg virus antigens, which were prepared on the basis of the designed stabilizing solution, preserved at less than 90% of its baseline activity during 10 months at a storage temperature of 2 to 8 degrees c. | 2010 | 20364672 |
| preparation and evaluation of thiomer nanoparticles via high pressure homogenization. | the aim of this study was to establish and evaluate a high pressure homogenization method for the preparation of thiomer nanoparticles. particles were formulated by incorporation of the model protein horseradish peroxidase in chitosan-glutathione (ch-gsh) and poly(acrylic acid)-glutathione (paa-gsh) via co-precipitation followed by air jet milling. the resulting microparticles were suspended in distilled water using an ultraturax and subsequently micronized by high pressure homogenization. final ... | 2010 | 20367028 |
| accessory olfactory bulb function is modulated by input from the main olfactory epithelium. | although it is now established that sensory neurons in both the main olfactory epithelium and the vomeronasal organ may be activated by both general and pheromonal odorants, it remains unclear what initiates sampling by the vomeronasal organ. anterograde transport of wheat germ agglutinin-horseradish peroxidase was used to determine that adequate intranasal syringing with zinc sulfate interrupted all inputs to the main olfactory bulb but left intact those to the accessory olfactory bulb. adult m ... | 2010 | 20377623 |
| disposable biosensors for determination of biogenic amines. | this work reports monoamine oxidase (mao)/horseradish peroxidase (hrp) and diamine oxidase (dao)/horseradish peroxidase (hrp) based biosensors using screen-printed carbon electrodes for the determination of biogenic amines (ba). the enzymes have been covalently immobilized onto the carbon working electrode, previously modified by an aryl diazonium salt, using hydroxysuccinimide and carbodiimide. the detection has been performed by measuring the cathodic current due to the reduction of the mediat ... | 2010 | 20381686 |
| development of an acetaminophen amperometric biosensor based on peroxidase entrapped in polyacrylamide microgels. | in this work, horseradish peroxidase (hrp) has been entrapped in cross-linked polyacrylamide microparticles using the concentrated emulsion polymerization method. the feasibility of amperometric detection of acetaminophen (apap) in a biosensor using this hrp immobilized system as the biological material in the presence of hydrogen peroxide was investigated. we found that the optimum microgel cross-linking degree required to retain the protein and to allow the diffusion of the phenolic drug onto ... | 2011 | 20382517 |
| [an enzyme sensor for phenolic compounds analysis]. | phenolic analysis was established on the immobilized horseradish peroxidase (hrp) catalyzed oxidation reaction. it subsequently catalyzed oxidative coupling of phenol with 4-aminophenazone using aqueous hydrogen peroxide to form intensely colored products for spectrophotometric analysis. hrps were trapped in sol-gel matrix in a mild procedure. the immobilized hrps maintain almost identical enzymatic activity as those in solution. bet analysis indicates that the silica itself is a porous structur ... | 2010 | 20384170 |
| [fabrication and evaluation of the enzyme immunosensor for rapid detection of vibrio parahaemolyticus based on chitosan-sio2 hybrid membrane]. | to improve the key technology of immunesensors in immobilizing bio-sensitive element and keeping its bioactivity, an enzyme immunosensor based on chitosan-sio(2) (cs-sio(2)) hybrid membrane was fabricated. to estimate the new immunosensor vibrio parahaemolyticus which was the main pathogens of aquatic products. | 2010 | 20388366 |
| bienzyme bionanomultilayer electrode for glucose biosensing based on functional carbon nanotubes and sugar-lectin biospecific interaction. | bienzyme bionanomultilayer electrode for glucose biosensing was constructed based on functional carbon nanotubes and sugar-lectin biospecific interaction through layer-by-layer (lbl) assembly. after being functionalized by wrapping with polyelectrolyte, multiwalled carbon nanotubes (mcnts) were water soluble and positively charged. mcnt-bienzyme bionanomultilayer electrode was then fabricated by lbl assembly of horseradish peroxidase (hrp) and glucose oxidase (god) on functional mcnt modified el ... | 2010 | 20388484 |
| development and fabrication of nanoporous silicon-based bioreactors within a microfluidic chip. | multi-scale lithography and cryogenic deep reactive ion etching techniques were used to create ensembles of nanoporous, picolitre volume, reaction vessels within a microfluidic system. the fabrication of these vessels is described and how this process can be used to tailor vessel porosity by controlling the width of slits that constitute the vessel pores is demonstrated. control of pore size allows the containment of nucleic acids and enzymes that are the foundation of biochemical reaction syste ... | 2010 | 20390137 |