Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
efficacy of using a combination of rendered protein products as an undegradable intake protein supplement for lactating, winter-calving, beef cows fed bromegrass hay.seventy-two (36 in each of two consecutive years) lactating, british-crossbred cows (609 +/- 19 kg) were used to evaluate effects of feeding a feather meal-blood meal combination on performance by beef cows fed grass hay. bromegrass hay (9.6% cp, dm basis) was offered ad libitum and intake was measured daily in individual calan electronic headgates. acclimation to calan gates began approximately 20 d after parturition, and treatments were initiated 21 d later. cows were assigned randomly to one ...200515583059
the complete nucleotide sequence and multipartite organization of the tobacco mitochondrial genome: comparative analysis of mitochondrial genomes in higher plants.tobacco is a valuable model system for investigating the origin of mitochondrial dna (mtdna) in amphidiploid plants and studying the genetic interaction between mitochondria and chloroplasts in the various functions of the plant cell. as a first step, we have determined the complete mtdna sequence of nicotiana tabacum. the mtdna of n. tabacum can be assumed to be a master circle (mc) of 430,597 bp. sequence comparison of a large number of clones revealed that there are four classes of boundaries ...200415583938
absence of hg transpiration by shoot after hg uptake by roots of six terrestrial plant species.in this paper we investigated if, and to what extent, six different plant species accumulate, translocate and emit mercury (hg) into the air. the hg uptake by roots, distribution of hg to the shoot and release of hg via shoots of garden pea, spring wheat, sugar beet, oil-seed rape, white clover and willow were investigated in a transpiration chamber. the airborne hg was trapped in a hopcalite trap or a gold trap. traps and plant materials were analysed for content of hg by cvaas. the results sho ...200515589647
detection of beet yellows virus by rt-pcr and immunocapture rt-pcr in tetragonia expansa and beta vulgaris.two sensitive methods, rt-pcr with phenol-extracted rna or triton x-100-released rna and immunocapture rt-pcr (ir-rt-pcr) were used for the detection of beet yellows virus (byv) in young and old leaves of tetragonia expansa and sugar beet (beta vulgaris) and in sugar beet roots. four oligonucleotide primer pairs proved suitable for the detection of byv. the release of byv rna with triton x-100 was shown to be a very effective and easy as compared to isolation of total rna by phenol extraction wi ...200415595212
suppression of phenylalanine ammonia lyase expression in sugar beet by the fungal pathogen cercospora beticola is mediated at the core promoter of the gene.the suppression of plant defence reactions plays a crucial role in causing plant diseases. in this report, we show that inducible plant defences are repressed during the development of cercospora leaf spot disease. in the early phase of infection of sugar beet (beta vulgaris l.) leaves with the phytopathogenic fungus cercospora beticola , a reduction in the expression of the phenylalanine ammonia lyase (bvpal) and cinnamic acid 4-hydroxylase (bvc4h) genes was observed. bvpal reduction was found ...200415604720
mechanism of luminal ca2+ and mg2+ action on the vacuolar slowly activating channels.the non-selective slow vacuolar (sv) channel can dominate tonoplast conductance, making it necessary to tightly control its activity. applying the patch-clamp technique to vacuoles from sugar beet (beta vulgaris l.) taproots we studied the effect of divalent cations on the vacuolar side of the sv channel. our results show that the sv channel has two independent binding sites for vacuolar divalent cations, (i) a less selective one, inside the channel pore, binding to which impedes channel conduct ...200415605179
xylanase production by trichoderma reesei rut c-30 grown on l-arabinose-rich plant hydrolysates.the suitability of l-arabinose-rich plant hydrolysates as carbon sources and inducers of xylanase production in trichoderma reesei rut c-30 was tested. significantly higher xylanase activities were obtained in cultures on oat husk and sugar beet pulp hydrolysates than on lactose. in batch culture with oat husk hydrolysate and lactose, the xylanase activity was about 9 times higher ( approximately 510 iu/ml) than in lactose ( approximately 60 iu/ml). even higher xylanase activity ( approximately ...200515607187
generation of organic acids and monosaccharides by hydrolytic and oxidative transformation of food processing residues.carbohydrate-rich biomass residues, i.e. sugar beet molasses, whey powder, wine yeast, potato peel sludge, spent hops, malt dust and apple marc, were tested as starting materials for the generation of marketable chemicals, e.g. aliphatic acids, sugar acids and mono-/disaccharides. residues were oxidized or hydrolyzed under acidic or alkaline conditions applying conventional laboratory digestion methods and microwave assisted techniques. yields and compositions of the oxidation products differed ...200515607197
[sugar beet photosynthesis under conditions of increasing water deficiency in soil and protective effects of a low-molecular-weight alcohol].the rate of co2 gas exchange, transpiration, stomatal conductivity, and efficiency of the use of water were assessed in leaves of the sugar beet (beta vulgaris var. saccharifera (alef) krass.) sprayed with 40% methanol, against the background of increasing water deficiency in soil. a decrease in the negative impact of water deficiency in experimental plants was shown to be due to the larger stress-resistance of the photosynthetic apparatus, higher rate of photosynthesis, and more effective use o ...201315609858
environmental implications of gene flow from sugar beet to wild beet--current status and future research needs.gene flow via seed or pollen is a basic biological process in plant evolution. the ecological and genetic consequences of gene flow depend on the amount and direction of gene flow as well as on the fitness of hybrids. the assessment of potential risks of transgenic plants should take into account the fact that conventional crops can often cross with wild plants. the precautionary approach in risk management of genetically modified plants (gmps) may make it necessary to monitor significant wild a ...200315612276
making the eu "risk window" transparent: the normative foundations of the environmental risk assessment of gmos.in europe, there seems to be widespread, morally based scepticism about the use of gmos in food production. in response to this scepticism, the revised eu directive 2001/18/ec on the deliberate release into the environment of genetically modified organisms stresses the importance of respecting ethical principles recognized in the member states. however, the directive fails to reflect the critical role of value judgements in scientific risk assessment and any subsequent approval procedure. in thi ...201515612414
a study on the reduction of hexavalent chromium in aqueous solutions by vinasse.hexavalent chromium introduced to the water ecosystem is an important issue due to the toxic effect it poses. the first step of conventional treatment of wastewaters containing hexavalent chromium is its reduction to chromium (iii) which is less toxic. the use of industrial waste material for the reduction of chromium (vi) may be a new cost-effective alternative. in this study, cr(vi) reduction characteristics of vinasse which is a waste material of alcohol production from sugar beet molasses wa ...200415617440
measured and computed volatilisation of the fungicide fenpropimorph from a sugar beet crop.depending on their vapour pressure, volatilisation can be one of the main pathways of emission of pesticides into the environment. the volatilisation of fenpropimorph was studied in a field experiment in which the fungicide was sprayed onto a sugar beet crop. volatilisation rates were calculated by measuring the concentration gradient in air, using the aerodynamic and bowen ratio methods. a simplified computation model was used to simulate pesticide volatilisation, together with the concurrent p ...200515619709
amelioration of indian urban air pollution phytotoxicity in beta vulgaris l. by modifying npk nutrients.air pollution levels are increasing at an alarming rate in many developing countries, including india and causing a potential threat to crop production. field experiments were conducted to examine the impact of urban air pollutants on biomass (yield) and some physiological and biochemical parameters of palak (beta vulgaris l. var. all green) that grew from germination to maturity at seven periurban sites of allahabad city having different concentrations of air pollutants under different levels o ...200515620584
salmonella enterica serovar kedougou contamination of commercially grown mushrooms.during investigation of an episode of salmonella enterica serovar kedougou contamination of mushrooms, multiple closely related isolates were obtained from mushrooms and mushroom-growing materials. contamination apparently originated from sugar beet lime, an alkaline material used in mushroom growing. no associated cases of human infection were detected.200515629233
chromatographic separation of nucleosides using a cross-linked xylose isomerase crystal stationary phase.cross-linked xylose isomerase (ec 5.3.1.5., from streptomyces rubiginosus) crystals (clxic) packed into a 7.8 x 300 mm steel column showed specific affinity towards uridine (urd), cytidine (cyd), adenosine (ado), guanosine (guo), and thymidine. these nucleosides eluted out of the clxic column in the same order as the corresponding nucleoside bases, indicating that the retention depends mainly on the base component of the molecule. the interaction of nucleosides with the clxic material was not ba ...200415638157
establishment of phagocytic cell lines from larval hemocytes of the beet armyworm, spodoptera exigua.hemolymph was taken from beet armyworm (spodoptera exigua) larvae and a new hemocyte cell line (sehe920-1a) was established by supplementing the culture medium with a reduced form of glutathione to avoid the activation of prophenoloxidase cascade. to evaluate the phagocytic ability of the sehe920-1a cells, polystyrene microspheres of two sizes (6.14 +/- 0.45 microm and 2.84 +/- 0.14 microm in diameter) and inactivated spores of an entomopathogenic microsporidium, vairimorpha sp. nis m12 (5.10 +/ ...200415638701
a clp gene homologue belonging to the crp gene family globally regulates lytic enzyme production, antimicrobial activity, and biological control activity expressed by lysobacter enzymogenes strain c3.lysobacter enzymogenes strain c3, a biological control agent for plant diseases, produces multiple extracellular hydrolytic enzymes and displays antimicrobial activity against various fungal and oomycetous species. however, little is known about the regulation of these enzymes or their roles in antimicrobial activity and biocontrol. a study was undertaken to identify mutants of strain c3 affected in extracellular enzyme production and to evaluate their biocontrol efficacy. a single mini-tn5-lacz ...200515640196
reuse of liquid, dewatered, and composted sewage sludge on agricultural land: effects of long-term application on soil and crop.to evaluate the effects of repeated sewage sludge applications in comparison to mineral fertilisers on a winter wheat-maize-sugar beet rotation, a field experiment on a silty-loam soil, in the eastern po valley (italy), was carried out since 1988. municipal-industrial wastewater sludge as anaerobically digested, belt filtered (dewatered), and composted with wheat straw, has been applied at 5 and 10 mg dm ha(-1)yr(-1). biosolids gave crop yields similar to the highest mineral fertiliser dressing. ...201315644237
nutrient digestibility in sheep fed diets containing roundup ready or conventional fodder beet, sugar beet, and beet pulp.the objective of this digestibility assessment was to determine whether there are significant differences in the digestibility of roundup ready (glyphosate-tolerant) and conventional sugar beet, fodder beet, and beet pulp produced from sugar beet varieties when fed to sheep (seven wethers per treatment group). three experiments were conducted in this assessment. experiment 1 (35 wethers) compared one glyphosate-tolerant fodder beet variety with four conventional varieties, exp. 2 (42 wethers) co ...200515644513
strategy to select and assess antagonistic bacteria for biological control of rhizoctonia solani kühn.a screening strategy was developed to assess the potential of plant-associated bacteria to control diseases caused by rhizoctonia solani kühn. about 434 already characterized antagonistic bacterial strains isolated from diverse plant species and microenvironments were evaluated for biocontrol and plant growth promotion by a hierarchical combination of assays. analyzing in vitro antagonism towards different rhizoctonia isolates resulted in a selection of 20 potential biocontrol agents. the strain ...200415644895
molecular characterization of vulmar1, a complete mariner transposon of sugar beet and diversity of mariner- and en/spm-like sequences in the genus beta.transposons of the tc1-mariner superfamily are widespread in eukaryotic genomes. we have isolated the mariner element vulmar1 from beta vulgaris l., which is 3909 bp long and bordered by perfect terminal inverted repeats of 32 bp with homology to terminal inverted repeats of transposons from soybean and rice. according to a characteristic amino acid signature, vulmar1 can be assigned to the dd39d group of mariner transposons. vulmar1 is flanked by a 5'-ta-3' target site duplication that is typic ...200415644978
pectic zymogram variation and pathogenicity of rhizoctonia solani ag-4 to bean (phaseolus vulgaris) isolates in isfahn, iran.rhizoctonia disease, caused by rhizoctonia solani is one of the most important fungal diseases in bean fields in isfahan, iran. bean plants showing stem and root cankers were collected and rhizoctonia-like fungi obtained from the samples were identified by anastomosis. pure cultures of bean isolates of r. solani were identified as ag-4. there were also ag-4 isolates from tomato, potato, cucumber, alfalfa and sugar beet in the areas sampled. a total of 163 isolates of r. solani ag-4 originating f ...200415645177
valine uptake in the tap root of sugar beet: a comparative analysis with sucrose uptake.given the lack of data on the absorption of amino acids in the tap root of beta vulgaris, we studied the uptake of valine and compared it with that of sucrose at the same concentration (1 mm). the uptake of both substrates shared some similar characteristics. in particular, the absorption in both cases was controlled by an active process as evidenced by the inhibitory effect of cccp and inhibitors of atpases (des, dccd, orthovanadate). both absorptions also involved the thiol and histidyl groups ...200415658801
use of a beet necrotic yellow vein virus rna-5-derived replicon as a new tool for gene expression.a new gene-expression system based on rna-5 of beet necrotic yellow vein virus (bnyvv) was constructed to allow the expression of recombinant proteins in virally infected cells. replication and expression levels of the rna-5-based replicon containing the green fluorescence protein (gfp) gene were compared with those obtained with the well-characterized rna-3-derived replicon (rep-3). when rna-3 and/or rna-4 bnyvv rnas were added to the inoculum, the expression levels of rna-5-encoded gfp were co ...200515659766
influence of olive mill wastewater in composting and impact of the compost on a swiss chard crop and soil properties.the suitability of olive mill wastewater (omw) for composting was studied by the addition of this liquid waste to a mixture of cotton gin waste and sewage sludge, and its composting was compared with that of another pile of similar composition, but without olive mill wastewater. both piles were composted by the rutgers static pile system in a pilot plant. to study the effects of both composts on plant yield and soil properties, a plot experiment was carried out with swiss chard (beta vulgaris l. ...200515661299
a multiplex rt-pcr assay capable of distinguishing beet necrotic yellow vein virus types a and b.a multiplex reverse-transcription polymerase chain assay (mrt-pcr) was developed, based on primers designed to distinguish the a and b types of beet necrotic yellow vein virus (bnyvv). rna was extracted from 72 bnyvv isolates from asia, europe and north america, and the type of each isolate determined using an established detection method based on single strand conformation polymorphisms (sscps). an area of the 'triple gene block' region on rna 2 was amplified and sequenced from 16 isolates of t ...200415664049
quantitative detection of the potato cyst nematode, globodera pallida, and the beet cyst nematode, heterodera schachtii, using real-time pcr with sybr green i dye.the potato cyst nematode globodera pallida and the beet cyst nematode heterodera schachtii are major nematode pests in world agriculture. precise identification and knowledge about the number of nematodes in field soil are necessary to develop effective integrated pest control. here we report the results of the real-time pcr assay for the rapid detection and quantification of g. pallida and h. schachtii. using species specific primers and sybr green i dye, we were able to detect a single second ...200515680208
comparison of the beet necrotic yellow vein virus p75 nucleotide sequences of belgian type a and type b sources.rna-2 p75 open reading frame sequencing was conducted on bnyvv-infected sugar beet plants originating from 10 belgian sources and was compared with sequences originating from france, kazakhstan, japan and china. this allowed the characterization of types a and b in belgium. sequence analysis confirmed that type a is intermediate between types p and b. the analysis of nucleotide sequences of the capsid protein epitopes and of different motifs involved in the transmission of the virus to the plant ...200515681050
inhibition of virus dna replication by artificial zinc finger proteins.prevention of virus infections is a major objective in agriculture and human health. one attractive approach to the prevention is inhibition of virus replication. to demonstrate this concept in vivo, an artificial zinc finger protein (azp) targeting the replication origin of the beet severe curly top virus (bsctv), a model dna virus, was created. in vitro dna binding assays indicated that the azp efficiently blocked binding of the viral replication protein (rep), which initiates virus replicatio ...200515681461
establishment of two ectomycorrhizal shrub species in a semiarid site after in situ amendment with sugar beet, rock phosphate, and aspergillus niger.a field experiment was carried out to assess the effectiveness of the addition of sugar beet, rock phosphate, and aspergillus niger directly into the planting hole, and the mycorrhizal inoculation of seedlings with scleroderma verrucosum, for promotion of plant growth of cistus albidus l. and quercus coccifera l. and enhancement of soil physicochemical, biochemical, and biological properties, in a degraded semiarid mediterranean area. one year after planting, the available phosphorus content in ...200515690228
assessment of genotypic diversity of antibiotic-producing pseudomonas species in the rhizosphere by denaturing gradient gel electrophoresis.the genotypic diversity of antibiotic-producing pseudomonas spp. provides an enormous resource for identifying strains that are highly rhizosphere competent and superior for biological control of plant diseases. in this study, a simple and rapid method was developed to determine the presence and genotypic diversity of 2,4-diacetylphloroglucinol (dapg)-producing pseudomonas strains in rhizosphere samples. denaturing gradient gel electrophoresis (dgge) of 350-bp fragments of phld, a key gene invol ...200515691958
management of genetically modified herbicide-tolerant sugar beet for spring and autumn environmental benefit.when used in genetically modified herbicide-tolerant (gmht) crops, glyphosate provides great flexibility to manipulate weed populations with consequences for invertebrates and higher trophic levels, for example birds. a range of timings of band and overall spray treatments of glyphosate to gmht sugar beet were compared with a conventional weed control programme in four field trials over 2 years. single overall sprays applied between 200 and 250 accumulated day degrees (above a base air temperatu ...200515695200
isolation and toxicity of bacillus thuringiensis from potato-growing areas in bolivia.bacillus thuringiensis was isolated from 116 samples collected in high altitude potato-growing areas in bolivia. in these regions, main potato pests are the potato tuberworm phthorimaea operculella, and the andean weevils premnotrypes latithorax and rhigopsidius tucumanus. b. thuringiensis was found in 60% of the samples. the main percentage of samples with b. thuringiensis was found in larvae of r. tucumanus (78%). bioassays were performed with 112 isolates. none resulted toxic to either larvae ...200515707864
proteome analysis of sugar beet leaves under drought stress.drought is one of the major factors limiting the yield of sugar beet (beta vulgaris l.). the identification of candidate genes for marker-assisted selection (mas) could greatly improve the efficiency of breeding for increased drought tolerance. drought-induced changes in the proteome could highlight important genes. two genotypes of sugar beet (7112 and 7219-p.69) differing in genetic background were cultivated in the field. a line-source sprinkler irrigation system was used to apply irrigated a ...200515712235
bacterial artificial chromosome-derived molecular markers for early bolting in sugar beet.early bolting in sugar beet (beta vulgaris l.) is controlled by the dominant gene b. from an incomplete physical map around the b gene, 18 bacterial artificial chromosomes (bacs) were selected for marker development. three bacs were shotgun-sequenced, and 61 open reading frames (orfs) were identified. together with 104 bac ends from 54 bacs, a total number of 55,464 nucleotides were sequenced. of these, 37 bac ends and 12 orfs were selected for marker development. thirty-one percent of the seque ...200515714328
action of 24-epibrassinolide on a cell line of the beet armyworm, spodoptera exigua.the spodoptera exigua cell line se4 is sensitive for ecdysteroid activity stimulated by the insect molting hormone, 20-hydroxyecdysone (20e), showing a cease in cell proliferation (with 50% inhibition around 1 microm) and characteristic cell morphology changes with aggregation and formation of long filamentous cytoplasmic extensions. the bisacylhydrazine tebufenozide also triggered such typical cellular effects in se4, and in addition, it showed an affinity for binding in competition with 3h-pon ...200515717319
heterotrophic high cell-density fed-batch cultures of the phycocyanin-producing red alga galdieria sulphuraria.growth and phycocyanin production in batch and fed-batch cultures of the microalga galdieria sulphuraria 074g, which was grown heterotrophically in darkness on glucose, fructose, sucrose, and sugar beet molasses, was investigated. in batch cultures, specific growth rates and yields of biomass dry weight on the pure sugars were 1.08-1.15 day-1 and 0.48-0.50 g g-1, respectively. they were slightly higher when molasses was the carbon source. cellular phycocyanin contents during the exponential grow ...200515723314
maize genes induced by herbivory and volicitin.in crop plants, both mechanical damage and insect attack trigger rapid changes in gene transcription. we investigated whether insect herbivory differs from a general wound response, and if so, is the induction specific to the pest/host plant interaction? herbivory by beet armyworm (baw; spodoptera exigua) caterpillars on maize results in a unique pattern of volatile compounds not triggered by wounding alone that attracts the generalist parasitoid cotesia marginiventris. caterpillar-induced volat ...200415724970
analysis of nucleotide sequences and multimeric forms of a novel satellite rna associated with beet black scorch virus.the full-length sequence of a satellite rna (sat-rna) of beet black scorch virus isolate x (bbsv-x) was determined. this agent is 615 nucleotides long and lacks extensive sequence homology with its helper virus or with other reported viruses. purified virus particles contained abundant single-stranded plus-sense monomers and smaller amounts of dimers. single-stranded rnas from total plant rna extracts also included primarily monomers and smaller amounts of dimers that could be revealed by hybrid ...200515731260
are seeds suitable for flow cytometric estimation of plant genome size?nuclear dna content in plants is commonly estimated using flow cytometry (fcm). plant material suitable for fcm measurement should contain the majority of its cells arrested in the g0/g1 phase of the cell cycle. usually young, rapidly growing leaves are used for analysis. however, in some cases seeds would be more convenient because they can be easily transported and analyzed without the delays and additional costs required to raise seedlings. using seeds would be particularly suitable for speci ...200515739186
application of aspergillus niger-treated agrowaste residue and glomus mosseae for improving growth and nutrition of trifolium repens in a cd-contaminated soil.the microbial transformation of sugar beet (sb) agrowaste with or without rock-phosphate (rp) has utility for the improvement of plant growth in a cd (5 microg g-1) artificially contaminated soil, particularly when the soil is co-inoculated with arbuscular mycorrhizal (am) fungus glomus mosseae isolated from a cd-polluted area. under such cd-polluted conditions, the limited growth, mineral nutrition, symbiotic developments (nodulation and am-colonization) and soil enzymatic activities were stimu ...200515748763
soil activity and persistence of sulcotrione and mesotrione.greenhouse bioassays were set up using a small pot test method to determine the intrinsic sensitivity of different plant species to sulcotrione and mesotrione applied in a sandy loam soil. herbicides were applied over an appropriate concentration range. after a 2-3 week test period, foliage fresh weight was determined. data were subjected to a non-lineair regression analysis. using the regression equations, ed50-values (herbicide concentrations that cause 50 percent foliage fresh weight reductio ...200415759393
use of high resolution digital thermography to detect heterodera schachtii infestation in sugar beets.thermography is a non-destructive method used to monitor pest and disease infestations, as it is related to changes in plant water status. surface temperature differences of the crop canopy may be an indicator of nematode infestation as the parasitation of the root system reduces evaporation of leaves. to test the potential of high resolution digital thermography to detect heterodera schachtii infestation, experiments using increasing nematode densities and different sugar beet varieties were co ...200415759435
occurrence of plant parasitic nematodes (tylenchina) in sugar beet fields in fars province, iran.on a survey of plant parasitic nematode fauna, belonging to the suborder tylenchina, about 110 soil and root samples were collected from different sugar beet field in fars province in iran. the samples were washed and the nematodes were extracted by centrifugal flotation technique according to the modified method by de grisse, 1969. they were fixed and transferred to glycerine, then permanent slides were mounted. morphological and morphometrical characters of collected species were carefully stu ...200415759441
production of transgenetic sugarbeet (beta vulgaris l.) plants resistant to phosphinothricin.a method of agrobacterium-mediated genetic transformation of sugarbeet (beta vulgaris l.) with vacuum infiltration has been developed. aseptic 3-weeks old etiolated seedlings of two diploid o-type sugarbeet lines (ks3 and ks7) have been used for genetic transformation. transgenic sugarbeet plants carrying the reporter beta-glucuronidase gene have been selected for their resistance to glufosinate ammonium herbicide. integration of transgenes into sugarbeet genome was confirmed with gus assay and ...200515763494
[the dynamics of hormonal status of developing red beet root (beta vulgaris l.) in correlation with the dynamics of sugar accumulation].we studied the dynamics of phytohormone levels (indole-3-acetic acid, abscisic acid, cytokinins, and gibberellin-like substances) in red beet (beta vulgaris l.) roots at different developmental stages in comparison with the data on the dynamics of sugar accumulation in order to test possible hormonal regulation of sugar accumulation. the obtained data suggest the involvement of cytokinins and gibberellin-like substances in the control of sugar accumulation in the roots, while indole-3-acetic aci ...201615768631
nonregulated expression of tgbp3 of hordei-like viruses but not of potex-like viruses inhibits beet necrotic yellow vein virus cell-to-cell movement.plant viruses containing a triple gene block (tgb) movement protein gene cassette fall into two classes. we have shown previously that the third tgb protein (tgbp3) of beet necrotic yellow vein virus (bnyvv; class 1) and peanut clump virus (class 1) inhibit bnyvv intercellular movement when expressed from a co-inoculated bnyvv rna 3-based replicon. here we show that autonomous expression of tgbp3's of four other class 1 viruses of various genera also inhibits bnyvv movement. no such effect was o ...200515770352
[production of transgenic sugarbeet (beta vulgaris l.) plants of o-type using agrobacterium tumefaciens].a method of agrobacterium-mediated genetic transformation of sugarbeet (beta vulgaris l.) by vacuum infiltration has been developed. transgenic sugarbeet plants of ukraine breeding were selected for their resistance either to the antibiotic kanamycin or to the herbicide glufosinate ammonium. integration of transgenes was confirmed by pcr and gus-assay.200415771083
evidence that rna silencing-mediated resistance to beet necrotic yellow vein virus is less effective in roots than in leaves.in plants, rna silencing is part of a defense mechanism against virus infection but there is little information as to whether rna silencing-mediated resistance functions similarly in roots and leaves. we have obtained transgenic nicotiana benthamiana plants encoding the coat protein readthrough domain open reading frame (54 kda) of beet necrotic yellow vein virus (bnyvv), which either showed a highly resistant or a recovery phenotype following foliar rub-inoculation with bnyvv. these phenotypes ...200515782633
site and extent of starch degradation in the dairy cow - a comparison between in vivo, in situ and in vitro measurements.prediction of the supply of glycogenic precursors to dairy cows and the site of degradation of wheat, maize and potato starch (ps) were determined in an in vivo experiment and the results were compared with data obtained from experiments involving in situ nylon bag and in vitro gas production techniques. in a latin square design experiment four lactating dairy cows fitted with a rumen cannula and t-piece cannulae in the duodenum and terminal ileum, received either a low-starch control diet or di ...200515787988
beet-vinasse (condensed molasses solubles) as an ingredient in diets for cattle and pigs - nutritive value and limitations.sugar beet vinasse, originating as the condensed molasses residue from yeast production, was fed to bulls and pigs to determine the feed value. it contained, per kg dry matter (dm content 66.8%), 293 g crude protein, 305 g n-free extract (therefrom 34.4 g total sugar), and 395 g crude ash. the experiment was carried out with three fattening bulls (german holstein) and four adult pigs (gottingen minipig). in bulls, two balance trials lasting 20 days each (10 days adaptation; 10 days collection) w ...200515787991
selection theory for marker-assisted backcrossing.marker-assisted backcrossing is routinely applied in breeding programs for gene introgression. while selection theory is the most important tool for the design of breeding programs for improvement of quantitative characters, no general selection theory is available for marker-assisted backcrossing. in this treatise, we develop a theory for marker-assisted selection for the proportion of the genome originating from the recurrent parent in a backcross program, carried out after preselection for th ...200515802512
assessment of novel foods in animal nutrition.composition of feeds from gmo crops were determined as well as digestion and feeding experiments were carried out with broilers (bt-corn), layers (bt-corn, pat-corn), pigs (bt-corn, pat-sugar beet, soybeans), sheep (bt-corn silage, pat-corn silage), growing bulls (bt-corn silage) and fistulated cows (bt-corn silage). up to now, no significant differences in nutritional value between feeds from isogenic and transgenic plants of the first generation were observed. the so-called substantial equival ...200315806924
an outbreak of pontiac fever with respiratory distress among workers performing high-pressure cleaning at a sugar-beet processing plant.in august 2000, the minnesota department of health was notified of and investigated an outbreak of febrile respiratory illness among workers at a sugar-beet processing plant.200515809913
optimization of conditions for the efficient production of mutan in streptococcal cultures and post-culture liquids.the strain streptococcus sobrinus ccug 21020 was found to produce water-insoluble and adhesive mutan. the factors influencing both stages of the mutan production, i.e. streptococcal cultures and glucan synthesis in post-culture supernatants were standardized. the application of optimized process parameters for mutan production on a larger scale made it possible to obtain approximately 2.2 g of water-insoluble glucan per 11 of culture supernate--this productivity was higher than the best reported ...200515813222
low level of gene flow from cultivated beets (beta vulgaris l. ssp. vulgaris) into danish populations of sea beet (beta vulgaris l. ssp. maritima (l.) arcangeli).gene flow from sugar beets to sea beets occurs in the seed propagation areas in southern europe. some seed propagation also takes place in denmark, but here the crop-wild gene flow has not been investigated. hence, we studied gene flow to sea beet populations from sugar beet lines used in danish seed propagation areas. a set of 12 danish, two swedish, one french, one italian, one dutch, and one irish populations of sea beets, and four lines of sugar beet were analysed. to evaluate the genetic va ...200515813779
effects of azadirachtin on beet soilborne pomovirus and soil biological properties on sugar beet.beet soilborne pomovirus (bsbv) is an important soilborne virus disease in growing areas of sugar beet. bsbv was transmitted by polymyxa betae keskin. effects of azadirachtin on bsbv and soil biological properties were studied under a greenhouse. the presence of bsbv was tested in soil samples using bait plant test and triple antibody sandwich-enzyme-linked immunosorbent assay (tas-elisa). the concentration of bsbv in sugar beet roots was significantly reduced by the application of azadirachtin ...200515825683
consumer acceptability of conjugated linoleic acid-enriched milk and cheddar cheese from cows grazing on pasture.two experiments were conducted to study the consumer acceptability attributes of conjugated linoleic acid (cla)-enriched milk and cheese from cows grazing on pasture. in experiment 1, 15 cows were fed either a diet containing 51% alfalfa hay plus corn silage and 49% concentrate [total mixed ration (tmr)], were grazed on pasture, or were grazed on pasture and received 3.2 kg/d of a grain mix. the grain mix contained 75% full-fat extruded soybeans (ffes), 10% corn, 10% beet pulp, and 5% molasses. ...200515829677
qtl mapping of bnyvv resistance from the wb41 source in sugar beet.the most important rhizomania-resistance gene in sugar beet is the rz1 gene from the holly sugar company in california, the source widely used to breed partially resistant varieties. other important gene sources are wb41 and wb42, which both originate from beta vulgaris subsp. maritima collected in denmark, and which have been reported to be similar. the major resistance gene in wb42 is known as rz2. we studied the resistance in wb41 and used markers to map the major resistance gene in this sour ...200515838550
abridged 5s rdna units in sea beet (beta vulgaris subsp. maritima).amplification by polymerase chain reaction of the 5s rdna repeat units of beta vulgaris subsp. maritima resulted in a 350-bp product corresponding to the full-length 5s unit, but also revealed 4 abridged unit classes, each with a deletion that removed most of the spacer and 12-76 bp of the coding sequence. each abridged type lacks at least 1 of the conserved elements involved in transcription of the 5s gene, and so appear to be nonfunctional. network analysis revealed that the abridged units are ...200515838559
the 5'-leader sequence of sugar beet mitochondrial atp6 encodes a novel polypeptide that is characteristic of owen cytoplasmic male sterility.cytoplasmic male sterility (cms) is a mitochondrially encoded trait, which is characterized by a failure of plants to produce viable pollen. we have investigated the protein profile of mitochondria from sugar beet plants with normal (fertile) or cms cytoplasm, and observed that a 35-kda polypeptide is expressed in owen cms plants but not in normal plants. the variant 35-kda polypeptide was found in cms mitochondria placed in five different nuclear backgrounds. interestingly, this polypeptide pro ...200515838639
evaluation of photo-mutagenicity and photo-cytotoxicity of food coloring agents.pigments extracted from natural products are widely used for food coloration in japan. an investigation concerning the photo-mutagenicity and photo-carcinogenicity of frequently used colorants in japan was performed. colorants examined were from laccifer lacca (lac-color), coccus cacti (cochineal-color), carthamus tinctorius (carthamus yellow), gardenia augusta (gardenia yellow and gardenia blue), monascus anka and monascus purpureus (monascus red), the skin of vitis vinifera and vitis labrusca ...200515843386
detection of sugar adulterants in apple juice using fourier transform infrared spectroscopy and chemometrics.fourier transform infrared spectroscopy and attenuated total reflection sampling have been used to detect adulteration of single strength apple juice samples. the sample set comprised 224 authentic apple juices and 480 adulterated samples. adulterants used included partially inverted cane syrup (pics), beet sucrose (bs), high fructose corn syrup (hfcs), and a synthetic solution of fructose, glucose, and sucrose (fgs). adulteration was carried out on individual apple juice samples at levels of 10 ...200515853360
formation of decarboxylated betacyanins in heated purified betacyanin fractions from red beet root (beta vulgaris l.) monitored by lc-ms/ms.mixtures of mono-, bi-, and tridecarboxylated betacyanins together with their corresponding neobetacyanins obtained from beta vulgaris l. root juice as heating degradation products of betacyanins were identified by high-performance liquid chromatography with tandem mass spectrometry (lc-ms/ms) and diode-array (lc-dad) detection. two monodecarboxy-betacyanin pairs of diastereomers were detected after the decarboxylation in ethanolic and aqueous solutions. generation of 17-decarboxy-betacyanins an ...200515853391
elucidation of the emulsification properties of sugar beet pectin.a protocol has been developed to fractionate sugar beet pectin using hydrophobic affinity chromatography. three samples eluted from the column using 4 m nacl as solvent (fractions 1a, 1b, and 1c), two fractions eluted using 2 m nacl (fractions 2a and 2b), and one fraction eluted using water (fraction 3). the fractions were shown to be very polydisperse, and differences between the gpc refractive index and uv absorbance (214 nm) elution profiles demonstrated chemical heterogeneity. they were foun ...200515853406
effects of different cultivation temperatures on plasma membrane atpase activity and lipid composition of sugar beet roots.sugar beet seedlings (beta vulgaris l. cv. monohill) were cultivated for 3 weeks at different root and shoot temperatures and the plasma membranes (pm) from roots were purified by aqueous two-phase partitioning and analyzed for lipid composition and atpase activities. lipid analyses, undertaken immediately after pm purification from the roots, showed that a low root zone temperature (10 degrees c) decreased the ratio between the major lipids phosphatidylcholine (pc) and phosphatidylethanolamine ...200515854834
titration biosensors for the estimation of the biochemical nitrate demand of municipal and industrial wastes.an anoxic titrimetric test was investigated for measuring denitrification potential of different wastewaters, both municipal and industrial, and to quantify the denitrifying activity in an activated sludge system. the method measures the amount of acid that is required to maintain the ph set-point value in a batch denitrification experiment, and it was performed using a denicon (denitrification controller) biosensor. the amount of acid is proportional to the nitrate used to oxidise the biodegrad ...200615856352
comparison of wild-type and uv-mutant beta-glucanase-producing strains of talaromyces emersonii with potential in brewing applications.a screen of 46 uv-mutant strains of the moderately thermophilic fungus talaromyces emersonii yielded two mutants (tc2, tc5) that displayed gross morphological differences to the parent strain and enhanced activity against mixed linkage cereal beta-glucans. activity against beta-(1, 3)(1, 4)-d: -glucan from barley (bbgase) was measured during growth of the mutant and wild-type strains on a variety of carbon sources, ranging from solka floc to crude cereal fractions. in liquid culture, tc2 and tc5 ...200515856354
tonoplast vesicles of beta vulgaris storage root show functional aquaporins regulated by protons.water is crucial for plant development and growth, and its transport pathways inside a plant are an ongoing topic for study. plants express a large number of membrane intrinsic proteins whose role is now being re-evaluated by considering not only the control of the overall plant water balance but also in adaptation to environmental challenges that may affect their physiology. in particular, we focused our work on water movements across the root cell tp (tonoplast), the delimiting membrane of the ...200515859949
molecular characterization and systemic induction of single-chain ribosome-inactivating proteins (rips) in sugar beet (beta vulgaris) leaves.sugar beet (beta vulgaris l.) leaves contain virus-inducible type 1 (single chain) ribosome-inactivating proteins that have been named beetins. the structural and functional characterization, the cellular location, and the potential role of beetins as antiviral agents are reported here. beetins are formed of a single polypeptide chain with a varying degree of glycosylation and strongly inhibited in vitro protein synthesis in rabbit reticulocyte lysates (ic50=1.15 ng ml(-1)) and a vicia sativa l. ...200515863448
biochemical characterization of silene alba alpha4-fucosyltransferase and lewis a products.alpha1,4-fucosylation has been recently detected in arabidopsis thaliana [leonard et al. (2002), glycobiology 12: 299-306], and corresponding enzymes have also been characterized in beta vulgaris [bakker et al. (2001), febs lett, 507: 307-312], and lycopersicum aesculentum [wilson (2001), glycoconjugate j., 18: 439-447]. here we demonstrated fucosyltransferase activity (fuct) in silene alba cells and tissues. the fuc linkage to glcnac residues of the lactosamine moiety of the type i acceptor was ...200515864437
changes in soil c-isotopic composition in an agroecosystem under free air carbon dioxide enrichment (face) treatment during a crop rotation period.face (free air carbon dioxide enrichment) has been used since 1999 to evaluate the effects of future atmospheric co(2) concentrations on an arable crop agroecosystem. the experiment conducted at the institute of agroecology at the federal research centre in braunschweig consists of a typical local crop rotation of winter barley, a cover crop, sugar beet and winter wheat. the atmospheric co2 concentration of ambient air is about 375 ppm with a delta13c value of -7 to -9 per thousand, and 550 ppm ...200515880658
distribution of pectic epitopes in cell walls of the sugar beet root.immunolabelling techniques with antibodies specific to partially methyl-esterified homogalacturonan (jim5: unesterified residues flanked by methylesterified residues. jim7: methyl-esterified residues flanked by unesterified residues), a blockwise de-esterified homogalacturonan (2f4), 1,4-galactan (lm5) and 1,5-arabinan (lm6) were used to map the distribution of pectin motifs in cell walls of sugar beet root (beta vulgaris). pme and alkali treatments of sections were used in conjunction with jim5 ...200515887026
a regional-scale study on the crop uptake of cadmium from sandy soils: measurement and modeling.plant uptake is one of the major pathways by which cadmium (cd) in soils enters the human food chain. this study was conducted to investigate the uptake of cd by crops from soils within the wastewater irrigation area (wia) of braunschweig (germany) and to develop a simple process-oriented model that is suited to predict cd uptake at the regional scale. the sandy soils within the wia (4300 ha) have received considerable loads of heavy metals by irrigation using municipal wastewater for up to 40 y ...200515888888
association of beet leafhopper (hemiptera: cicadellidae) with a clover proliferation group phytoplasma in columbia basin of washington and oregon.at least 16 taxa of cicadellids and delphacids were tested by polymerase chain reaction (pcr) for the presence of a phytoplasma in the clover proliferation group, designated 16srvi. nucleic acid extracts from individual insects or groups of 5-10 were tested using pcr primers designed from the dna sequence of 16s-23s rrna or ribosomal protein genes of the pathogen. the beet leafhopper, circulifer tenellus (baker), was most often associated with the phytoplasma, with approximately 16% of the insec ...200515889714
effect of aging on pheromone emission from a commercial beet armyworm (lepidoptera: noctuidae) lure and trap efficiency.the effect of aging on a commercial pheromone-based lure for attractiveness to male adult beet armyworms, spodoptera exigua (hübner), was assessed in terms of trapping efficiency and volatile emissions of two key components: (z,e)-9,12-tetradecadienyl acetate and (z)-9-tetradecen-1-ol. in field assays conducted in the lower rio grande valley, texas, for 9 wk (june-august 2000) and for 8 wk (march-may) in tamaulipas, mexico, 0-1-wk-old lures collected greater than four-fold more male beet armywor ...200515889726
uptake of sodium in quince, sugar beet, and wheat protoplasts determined by the fluorescent sodium-binding dye benzofuran isophthalate.the uptake of sodium into protoplasts of quince (cydonia oblonga mill, clone ba29), sugar beet (beta vulgaris l. cv. monohill), and wheat (triticum aestivum l. cv. kadett) was determined by use of the acetoxy methyl ester of the fluorescent sodium-binding benzofuran isopthalate (sbfi-am). in the presence of 1 mm cacl2, little sodium was taken up in the cytosol of quince mesophyll cells compared to cytosols of sugar beet and wheat. upon addition of 40 mm nacl, approximately the same amount of sod ...200515900884
adenosine kinase inhibition and suppression of rna silencing by geminivirus al2 and l2 proteins.most plant viruses are initiators and targets of rna silencing and encode proteins that suppress this adaptive host defense. the dna-containing geminiviruses are no exception, and the al2 protein (also known as ac2, c2, and transcriptional activator protein) encoded by members of the genus begomovirus has been shown to act as a silencing suppressor. here, a three-component, agrobacterium-mediated transient assay is used to further examine the silencing suppression activity of al2 from tomato gol ...200515919897
[enterosorption with pectin-containing medication in the treatment of peritonitis].one hundred and fifty patients aged 19 to 86 years with general peritonitis mainly in a toxic (85.3%) stage were examined. the leading causes of peritonitis were acute appendicitis (24.0%) and perforation of gastroduodenal ulcers (21.3%). three groups of patients were formed with a pair samples method for a comparative analysis of treatment200515940172
molecular mapping of a fertility restorer gene for owen cytoplasmic male sterility in sugar beet.we report here the molecular mapping of a fertility restorer gene (named rf1) for owen cytoplasmic male sterility in sugar beet. eight aflp and two rapd markers, tightly linked to the rf1 locus, were identified using bulked segregant analysis. three aflp markers, mafem972, mafem976 and mafem985, were found to co-segregate with the rf1 allele in our mapping populations. with the help of rflp markers, previously mapped on the sugar beet genome, we showed that rf1 is positioned in the terminal regi ...200515942757
sugar beet ca-pectate gel as a cost-effective immobilized-cell matrix for metal biosorption.whole bacterial (pseudomonas putida) and yeast (saccharomyces cerevisiae) cells were immobilized by entrapment in gel beads of ca-pectate obtained from sugar beet pulp and gel beads of commercial ca-alginate (of algal origin). these immobilized-cell particles were tested for cd2+ removal from dilute aqueous solutions and their mechanical properties were evaluated. only ca-pectate gel beads loaded with yeast cells displayed enhanced cadmium-binding efficiency (13.8 mg cd2+ per g dry matter) as co ...200115954604
the presence of acetobacter sp. in ensiled forage crops and ensiled industrial byproducts.the presence of acetic acid bacteria (aab) in whole crop maize silage, whole crop wheat silage, pressed sugar beet pulp silage, grass silage and brewer's grains silage was investigated. aab could be isolated from whole crop maize silage, whole crop wheat silage and pressed sugar beet pulp silage, but could not be detected in grass silage (> 100 silo's tested) or brewer's grains silage (5 silo's tested). thirty aab isolates were characterized to genus level. all isolates, i.e. 20 from whole crop ...200115954628
functional characterization of the beet necrotic yellow vein virus rna-5-encoded p26 protein: evidence for structural pathogenicity determinants.a beet necrotic yellow vein virus isolate containing a fifth rna is present in the pithiviers area of france. a full-length cdna clone of rna-5 was obtained and placed under the control of a t(7)-rna-pol promoter that allowed the production of infectious transcripts. "pithiviers" isolate-specific necrotic symptoms were obtained on chenopodium quinoa when rna-5-encoded p26 was expressed either from rna-5 or from an rna-3-derived replicon. by using haemagglutinin- and green fluorescent protein-tag ...200515958690
plant gamma-glutamyl hydrolases and folate polyglutamates: characterization, compartmentation, and co-occurrence in vacuoles.gamma-glutamyl hydrolase (ggh, ec 3.4.19.9) catalyzes removal of the polyglutamyl tail from folyl and p-aminobenzoyl polyglutamates. plants typically have one or a few ggh genes; arabidopsis has three, tandemly arranged on chromosome 1, which encode proteins with predicted secretory pathway signal peptides. two representative arabidopsis ggh proteins, atggh1 and atggh2 (the at1g78660 and at1g78680 gene products, respectively) were expressed in truncated form in escherichia coli and purified. bot ...200515961386
[the relationship between activities of chitinase and beta-1,3-glucanase and resistance to rhizomania in sugar beet].the relationship between activities of chitinase and beta-1,3-glucanase and resistance to rhizomania of sugar beet were studied in diseased soil and disease-free soil by using a method of comparative physiology. two resistant varieties and two susceptible ones of sugar beet were used in this experiment. chitinase and beta-1,3-glucanase activities of the resistant varieties were higher than the susceptible ones in either diseased soil or disease-free soil. there is a positive correlation between ...200515961903
urinary pharmacokinetics of betalains following consumption of red beet juice in healthy humans.the aim of the present pilot study was to characterise the renal elimination of betalains after consumption of red beet juice (rbj). six healthy, non-smoking female volunteers were given a single oral dose of either 500 ml of a commercial rbj containing 362.7 mg of betalains and 500 ml of tap water, respectively, in a sequential manner. urine was collected in intervals up to 24 h post-dose. renal excretion of betalains was determined spectrophotometrically and quantified as betanin-equivalents. ...200515964200
isolation, characterization, and cloning of {alpha}-l-arabinofuranosidase expressed during fruit ripening of japanese pear.alpha-l-arabinofuranosidase (alpha-l-arafase) was purified from fruit of japanese pear (pyrus pyrifolia). the enzyme solubilized from the cell wall by nacl and triton x-100 had the homogeneity of a single 62-kd polypeptide on sds-page after purification through the steps of hydroxyapatite, anion-exchange chromatography, and size-exclusion chromatography. a related cdna clone was isolated (pparf2). the transcript and related protein were detected solely in the ripening fruit corresponding to the ...200515965025
sequence divergence of four soilborne sugarbeet-infecting viruses.soilborne viruses are among the most harmful pathogens of sugarbeet (beta vulgaris l.ssp. vulgaris) but most of them lack information on genetic variability due to paucity of sequence data. only one isolate of beet soil borne virus (bsbv; genus pomovirus), beet virus q (bvq; genus pomovirus) and beet soil borne mosaic virus (bsbmv; genus benyvirus) has been characterised for the coat protein (cp) gene. in this study, the cp gene sequences of three isolates each of bsbv and beet necrotic yellow v ...200515965609
[using single primer pcr to amplify the orf25 gene fragment related to plant male-sterility in salicornia europaea].the gene orf25 encodes functional protein that may play an important role in plant fertility control in nature. to clone the orf25 from salicornia europaea xinjiang into a t-vector, a single designed primer was used to amplify 1.7kb cdna fragment with rt-pcr. sequence analysis reveals that the cloned fragment contains entire orf25 coding region with 98%, 95%, 92% and 88% identity to that of orf25 from beta vulgaris, nicotiana, wheat and maize mitochondrion, respectively. this analysis suggests t ...200315969049
dihydrocaffeoyl polyamines (kukoamine and allies) in potato (solanum tuberosum) tubers detected during metabolite profiling.four related phenolic amides previously undescribed from the species were revealed during metabolic profiling of potato (solanum tuberosum) tubers. n(1),n(12)-bis(dihydrocaffeoyl)spermine (kukoamine a) and n(1),n(8)-bis(dihydrocaffeoyl)spermidine were positively identified by comparison with authentic standards, while the structures n(1),n(4),n(12)-tris(dihydrocaffeoyl)spermine and n(1),n(4),n(8)-tris(dihydrocaffeoyl)spermidine are proposed for the other two metabolites. each amide was present a ...200515969534
[dw2, a new mutation of beet beta vulgaris l].twelve dwarf plants were found in the second hybrid generation of beet. the average height of mutant plants was 21.8 cm, their leaf blades and flowers were significantly smaller than normal, and the plants exhibited male and female sterility. this dwarfism was shown to be caused by a mutation differing from that previously described in beet, which is named dwarf2 (dw2). the experimental evidence suggests that this mutation appeared in one of the first-generation plants. based on plant phenotype ...200515977817
bacillus thuringiensis cry1ca-resistant spodoptera exigua lacks expression of one of four aminopeptidase n genes.insecticidal toxins from bacillus thuringiensis bind to receptors on midgut epithelial cells of susceptible insect larvae. aminopeptidases n (apns) from several insect species have been shown to be putative receptors for these toxins. here we report the cloning and expression analysis of four apn cdnas from spodoptera exigua.200515978131
adsorption of phenol from aqueous solution by using carbonised beet pulp.the beet pulp, a major low value by-product in sugar industry was used to prepare carbon for phenol adsorption. it was produced by carbonisation in n2 atmosphere at 600 degrees c for 1.5 h. the surface area of beet pulp carbon was measured as 47.5 m2g(-1) by using bet method. the adsorption studies of phenol from aqueous solution on beet pulp carbon (bpc) have been studied in the range of 25-500 mgdm(-3) initial phenol concentrations and at the temperatures of 25, 40 and 60 degrees c. the maximu ...200515990225
interactions of alpha-amylase and calcium chelator during neutral detergent fiber analysis.amylase and calcium chelators, such as disodium ethylene diaminotetraacetate (edta), are used in analysis of neutral detergent fiber (ndf) to dissolve starch and pectin, respectively. however, these reagents may interfere with each other's activity. six combinations of alpha-amylase and edta were examined for determining ndf values of beet pulp (beta vulgaris), ground corn (zea mays l.), timothy hay (phleum pratense), and soybean meal (glycine max l). for treatment a, 2.5 ml of alpha-amylase was ...200515998138
suppression of damping-off disease in host plants by the rhizoplane bacterium lysobacter sp. strain sb-k88 is linked to plant colonization and antibiosis against soilborne peronosporomycetes.we previously demonstrated that xanthobaccin a from the rhizoplane bacterium lysobacter sp. strain sb-k88 suppresses damping-off disease caused by pythium sp. in sugar beet. in this study we focused on modes of lysobacter sp. strain sb-k88 root colonization and antibiosis of the bacterium against aphanomyces cochlioides, a pathogen of damping-off disease. scanning electron microscopic analysis of 2-week-old sugar beet seedlings from seeds previously inoculated with sb-k88 revealed dense coloniza ...200516000790
genes involved in cyclic lipopeptide production are important for seed and straw colonization by pseudomonas sp. strain dss73.survival in natural bulk soil and colonization of sugar beet seeds and barley straw residues were determined for pseudomonas sp. strain dss73 and tn5 mutants in amsy (encoding a peptide synthetase involved in production of the cyclic lipopeptide amphisin) and gacs (encoding the sensory kinase of the two-component gaca/gacs regulatory system). no differences in survival or growth in response to carbon amendment (citrate) were observed in bulk soil. however, both mutants were impaired in their col ...200516000829
soil amendments reduce trace element solubility in a contaminated soil and allow regrowth of natural vegetation.we tested the effects of three amendments (a biosolid compost, a sugar beet lime, and a combination of leonardite plus sugar beet lime) on trace element stabilisation and spontaneous revegetation of a trace element contaminated soil. soil properties were analysed before and after amendment application. spontaneous vegetation growing on the experimental plot was studied by three surveys in terms of number of taxa colonising, percentage vegetation cover and plant biomass. macronutrients and trace ...200616005126
evidence for a common regulation in the activation of a polyphenol oxidase by trypsin and sodium dodecyl sulfate.polyphenol oxidase (ppo) was extracted from beet root, in both soluble and membrane fractions, and in both cases the enzyme was in a latent state. ppo from the membrane fraction showed no diphenolase activity unless it was activated by trypsin or sodium dodecyl sulfate (sds). the kinetics of the activation process of latent ppo by trypsin was studied and the specific rate constant of active ppo formation, k 3 , showed a value of 0.03 s(-1). the protease-activated form showed a ph optimum (6.5) a ...200516006247
changes in carotenoid content and distribution in living plant tissue can be observed and mapped in situ using nir-ft-raman spectroscopy.near-infrared (nir) excited fourier transform (ft) raman spectroscopy has been applied for in situ analysis of carotenoids in living plant samples. pelargonium x hortorum leaf has been mapped using a raman mapping technique to illustrate heterogeneous distribution of carotenoids. mapping has also been employed for visualization of carotenoid changes induced by abiotic and biotic stress. in a tomato (lycopersicon esculentum mill.) fruit, inhibition of lycopene biosynthesis and accumulation of bet ...200516007452
Displaying items 1701 - 1800 of 4531