Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| effects of melatonin and epiphyseal proteins on fluoride-induced adverse changes in antioxidant status of heart, liver, and kidney of rats. | several experimental and clinical reports indicated the oxidative stress-mediated adverse changes in vital organs of human and animal in fluoride (f) toxicity. therefore, the present study was undertaken to evaluate the therapeutic effect of buffalo (bubalus bubalis) epiphyseal (pineal) proteins (bep) and melatonin (mel) against f-induced oxidative stress in heart, liver, and kidney of experimental adult female rats. to accomplish this experimental objective, twenty-four adult female wistar rats ... | 2014 | 24790596 |
| neospora caninum serostatus is affected by age and species variables in cohabiting water buffaloes and beef cattle. | the aim of this study was to investigate how neospora caninum serostatus may be affected by variables such as host species (water buffaloes or cattle) and age in animals cohabiting in the same ranch. a convenience cross-sectional study was performed on four ranches in the northeast of argentina, where water buffalo are cohabitating with beef cattle. blood samples were collected from 1350 female water buffaloes (bubalus bubalis) and 880 female beef cattle (bos taurus and bos indicus crossbreeds) ... | 2014 | 24792747 |
| first cloned swamp buffalo produced from adult ear fibroblast cell. | the world's first cloned swamp buffalo (bubalus bubalis) derived from adult ear skin fibroblast has been reported. donor fibroblast cells were produced from biopsies taken from adult male ear skin and in vitro matured oocytes obtained from a slaughterhouse were used as cytoplasts. a total of 39 blastocysts and 19 morulae fresh embryos were transferred into 12 recipient buffaloes. progesterone assays indicated establishment of pregnancy in 10 of the 12 buffaloes (83.3%) after 45 days, with six an ... | 2014 | 24804579 |
| histology and ultrastructure of the lymph nodes of the buffalo (bos bubalus). | pre-scapular, femoral and mesenteric lymph nodes from five buffalo calves and five buffalo bulls were studied using light and transmission electron microscopy. the nodes were surrounded with a thin capsule of dense connective tissue and smooth muscles. subcapsular and trabecular lymphatic sinuses were lined with endothelial cells resting on a basement membrane. the cortex was formed by lymphoid follicles and inter-follicular lymphocytes. primary and secondary follicles were observed. the medulla ... | 2015 | 24810142 |
| serum biochemical and haematological reference intervals for water buffalo bubalus bubalis heifers. | based on a review of the literature, reference intervals for water buffalo (bubalus bubalis) serum biochemistry and haematology have not previously been published. the current study was done to establish reference intervals for water buffalo heifers. the international federation of clinical chemistry stated that at least 120 values are necessary to obtain reliable estimates for reference intervals. a total number of 127 clinically healthy buffalo heifers (1-2 years old) were included in the stud ... | 2014 | 24831856 |
| energy metabolites, lipid variables and lactation performance of periparturient murrah buffaloes (bubalus bubalis) fed on diet supplemented with inorganic chromium. | the aim of this study was to elucidate the effects of chromium (cr) supplementation as inorganic cr (crcl3·6h2o) on energy balance, lipid peroxidation, and lactation performance in periparturient murrah buffaloes. twenty-four multiparous murrah buffaloes according to lactation, parity, body mass, and expected calving date were divided equally. experimental buffaloes were randomly assigned to four treatment diets: a control diet and three diets with an inorganic cr supplementation at 0.5, 1.0, an ... | 2014 | 24833279 |
| transmission of foot-and-mouth disease virus from experimentally infected indian buffalo (bubalus bubalis) to in-contact naïve and vaccinated indian buffalo and cattle. | this study investigated the transmission of foot-and-mouth disease virus (fmdv) from experimentally infected indian buffalo to in-contact naïve and vaccinated cattle and buffalo. in each of six rooms, two donor buffalo that had been inoculated with fmdv were housed for five days with four recipient animals, comprising one vaccinated buffalo, one vaccinated calf, one unvaccinated buffalo and one unvaccinated calf. vaccination was carried out with current indian vaccine strain (o/ind/r2/75) and ch ... | 2014 | 24837776 |
| molecular cloning and characterization of a novel heat shock protein 20 of babesia orientalis. | the heat shock protein 20 (hsp20) gene of babesia orientalis (bohsp20) was identified from both genomic dna and cdna. the full-length bohsp20 gene was 690bp with one intron from position 88-243bp. the amplicon obtained from cdna corresponded to a full-length open reading frame (orf) with a length of 534bp, encoding a polypeptide of 178 amino acid residues with a predicted size of 20kda. the orf was cloned into a pet-28a plasmid and subsequently expressed as a his-fusion protein. the recombinant ... | 2014 | 24857770 |
| morphogenesis of mammary glands in buffalo (bubalus bubalis). | the present research was elucidated on the morphogenesis of mammary gland of buffalo during prenatal development. total of 16 foetuses ranging from 1.2 cm (34 days) to 108 cm cvrl (curved crown rump length) (317 days) were used for study. the study revealed that mammary line was first observed at 1.2 cm cvrl (34 days), mammary hillock at 1.7 cm (37 days), and mammary bud at 2.6 cm cvrl (41 days) foetuses. epidermal cone was found at 6.7 cm cvrl (58 days) whereas primary and secondary ducts were ... | 2014 | 24876967 |
| leuconostoc mesenteroides sjrp55: a bacteriocinogenic strain isolated from brazilian water buffalo mozzarella cheese. | the production of bacteriocins by leuconostoc mesenteroides represents an important opportunity for exploration of their potential use for industrial purpose. the antimicrobial compounds produced by l. mesenteroides subsp. mesenteroides sjrp55 strain were characterized and purified. cell-free supernatant of leuc. mesenteroides subsp. mesenteroides sjrp55 produced antibacterial compounds against listeria spp. strains and not inhibiting against lactobacillus spp. the antimicrobial substances were ... | 2014 | 24907159 |
| effects of trichostatin a on in vitro development and dna methylation level of the satellite i region of swamp buffalo (bubalus bubalis) cloned embryos. | trichostatin a (tsa), a histone deacetylase inhibitor, has been widely used to improve the cloning efficiency in several species. this brings our attention to investigation of the effects of tsa on developmental potential of swamp buffalo cloned embryos. swamp buffalo cloned embryos were produced by electrical pulse fusion of male swamp buffalo fibroblasts with swamp buffalo enucleated oocytes. after fusion, reconstructed oocytes were treated with 0, 25 or 50 nm tsa for 10 h. the results showed ... | 2014 | 24909601 |
| evaluation of antifreeze protein iii for cryopreservation of nili-ravi (bubalus bubalis) buffalo bull sperm. | lower fertility in buffaloes with frozen-thawed semen is attributed to sperm damage that is believed to be due to formation of ice crystals during freeze/thaw process. it was hypothesized that antifreeze proteins in the extender may improve the post thaw quality of buffalo bull sperm. for this purpose, two separate experiments were conducted to evaluate antifreeze proteins iii (afp iii) at 0 (control), 0.1, 1 and 10 μg/ml (experiment i) and 0 (control), 0.01, 0.1 and 1 μg/ml (experiment ii) for ... | 2014 | 24925471 |
| identification of fluoroquinolone-resistant extended-spectrum β-lactamase (ctx-m-8)-producing escherichia coli st224, st2179 and st2308 in buffalo (bubalus bubalis). | 2014 | 24928853 | |
| detection of copy number variations and their effects in chinese bulls. | copy number variations (cnvs) are a main source of genomic structural variations underlying animal evolution and production traits. here, with one pure-blooded angus bull as reference, we describe a genome-wide analysis of cnvs based on comparative genomic hybridization arrays in 29 chinese domesticated bulls and examined their effects on gene expression and cattle growth traits. | 2014 | 24935859 |
| developmental competence and expression pattern of bubaline (bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro. | to determine the direct effect of physiologically relevant high temperatures (40.5 and 41.5 °c) for two time periods (12 and 24 h) on bubaline oocytes during in vitro maturation. | 2014 | 24938361 |
| first report of isolation and molecular characterization of bubaline herpesvirus 1 (buhv1) from argentinean water buffaloes. | herpesviruses have mainly co-evolved with their hosts for millions of years. however, bovine herpesvirus 1 (bohv1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. bubaline herpesvirus 1 (buhv1) is an alphaherpesvirus closely related to bohv1 and bohv5. according to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of bohv1-related virus infection in wild and domestic ruminant species. rece ... | 2014 | 24938487 |
| detection of sex chromosome aneuploidy in dog spermatozoa by triple color fluorescence in situ hybridization. | with the development of a direct visualization of sex chromosome in a single sperm by fluorescence in situ hybridization (fish) technique, the frequency of aberration (aneuploidy) in spermatozoa in several mammals has been investigated. however, there is no report in the incidence of x-y aneuploidy in the sperm population of dogs. therefore, in this study, the aneuploidy in dog spermatozoa was examined by multicolor fish using specific molecular probes for canine sex chromosomes and autosome. se ... | 2014 | 24962971 |
| construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (bubalus bubalis) mammary epithelial cell line. | the aim of the present study was construction of mammary gland specific expression vector for high level of human insulin (hins) expression in transgenic buffalo for therapeutic use. we have constructed mammary gland specific vector containing human insulin gene and there expression efficiency was checked into in vitro cultured buffalo mammary epithelial cells (bumecs). human pro-insulin coding region was isolated from human genomic dna by intron skipping pcr primer and furin cleavage site was i ... | 2014 | 24969480 |
| bovine herpesvirus type 1 marker vaccine induces cross-protection against bubaline herpesvirus type 1 in water buffalo. | water buffalo (bubalus bubalis) are susceptible to bovine herpesvirus type 1 (bohv-1) and a species-specific herpesvirus, bubaline herpesvirus type 1 (buhv-1). in this study, an attenuated marker bohv-1 based vaccine against buhv-1 challenge was evaluated to determine whether it induces protection from viral replication. one group of water buffalo calves was immunized with an attenuated bohv-1 marker vaccine. a second group was not vaccinated and used as the control. during the post-vaccination ... | 2014 | 24985155 |
| insights into resistome and stress responses genes in bubalus bubalis rumen through metagenomic analysis. | buffalo rumen microbiota experience variety of diets and represents a huge reservoir of mobilome, resistome and stress responses. however, knowledge of metagenomic responses to such conditions is still rudimentary. we analyzed the metagenomes of buffalo rumen in the liquid and solid phase of the rumen biomaterial from river buffalo adapted to varying proportion of concentrate to green or dry roughages, using high-throughput sequencing to know the occurrence of antibiotics resistance genes, genet ... | 2014 | 24985977 |
| expression and antigenic characterization of bubaline herpesvirus 1 (buhv1) glycoprotein e and its potential application in the epidemiology and control of alphaherpesvirus infections in mediterranean water buffalo. | bubaline herpesvirus 1 (buhv1) is a member of ruminant alphaherpesviruses antigenically related to bovine herpesvirus 1 (bohv1). the impact of buhv1 infection in infectious bovine rhinotracheitis control program is difficult to establish, due to the lack of specific diagnostic test. the ectodomain of glycoprotein e of buhv1 was expressed as recombinant secreted protein and used in indirect elisa as well as in a discriminatory test using the bohv1 counterpart. a panel of monoclonal antibodies was ... | 2014 | 24992670 |
| genome-wide profiling of sperm dna methylation in relation to buffalo (bubalus bubalis) bull fertility. | the dna methylation pattern in spermatozoa of buffalo bulls of different fertility status was investigated. spermatozoa isolated dna from two groups of buffalo bulls (n = 5), selected based on their artificial insemination-generated conception rate data followed by ivf efficiency, were studied for global methylation changes using a custom-designed 180 k buffalo (bubalus bubalis) cpg island/promoter microarray. a total of 96 individual genes with another 55 genes covered under cpg islands were fo ... | 2014 | 25023295 |
| identity of sarcocystis species of the water buffalo (bubalus bubalis) and cattle (bos taurus) and the suppression of sarcocystis sinensis as a nomen nudum. | there are uncertainties concerning the identity and host species specificity of sarcocystis species of the water buffalo (bubalus bubalis) and cattle (bos taurus). currently, in cattle three species are recognized with known endogenous stages, viz.: s. cruzi (with canine definitive host), s. hirsuta (feline definitive host), and s. hominis (primate definitive host). recently, a fourth sarcocystis species with an unknown life cycle has been reported from cattle. in the water buffalo, four species ... | 2014 | 25034134 |
| water buffalo genome characterization by the illumina bovinehd beadchip. | to define the best strategies for genomic association studies and genomic selection, it is necessary to determine the extent of linkage disequilibrium (ld) and the genetic structure of the study population. the current study evaluated the transference of genomic information contained in the illumina bovinehd beadchip from cattle to buffaloes, and assessed the extent of the ld in buffaloes. of the 688,593 bovine single nucleotide polymorphism (snp) that were successfully genotyped from the 384 bu ... | 2014 | 25036164 |
| body condition, energy balance and immune status of periparturient murrah buffaloes (bubalus bubalis) supplemented with inorganic chromium. | periparturient murrah buffaloes were used to determine whether body condition, energy balance and immune status are affected by inorganic cr supplementation. twenty-four murrah buffaloes were blocked into four groups having six animals in each group and fed for 60 days pre-partum to 150 days post-partum. feeding regimen was same in all the groups except that these were supplemented with 0.0, 0.5, 1.0 and 1.5 of cr per kilogram of dry matter (dm) in the four respective groups. buffaloes were weig ... | 2014 | 25037066 |
| evaluation of milk trace elements, lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase activity of subclinical mastitis as and indicator of subclinical mastitis in riverine buffalo (bubalus bubalis). | mastitis is a highly morbid disease that requires detection at the subclinical stage. tropical countries like india mainly depend on milch buffaloes for milk. the present study was conducted to investigate whether the trace minerals viz. copper (cu), iron (fe), zinc (zn), cobalt (co) and manganese (mn) and enzyme activity of lactate dehydrogenase (ldh), alkaline phosphatase (alp) and aspartate aminotransferase (ast) in riverine buffalo milk can be used as an indicator of subclinical mastitis (sc ... | 2012 | 25049573 |
| differential expression of th1- and th2- type cytokines in peripheral blood mononuclear cells of murrah buffalo (bubalus bubalis) on tlr2 induction by b. subtilis peptidoglycan. | peripheral blood mononuclear cells (pbmcs) discriminate microbial pathogens and induce t-cell responses of appropriate effector phenotype accordingly. toll-like receptors (tlrs), in part, mediate this microbial recognition and differentiation while the development of t-cell effector functions critically depends on the release of th1- or th2- type cytokines. in the present study, buffalo pbmcs were stimulated under in vitro culture conditions by bacillus subtilis cell wall petidoglycan, a tlr2 li ... | 2012 | 25049659 |
| effect of ground corn cob replacement for cassava chip on feed intake, rumen fermentation and urinary derivatives in swamp buffaloes. | four thai - rumen fistulated male swamp buffaloes (bubalus bubalis), about four years old with 400±20 kg liveweight, were randomly assigned according to a 4×4 latin square design to receive dietary treatments. the treatments were: ground corn cob (gcc) replacement for cassava chip (cc) in concentrate at 0% (t1); gcc replacement at 33% (t2); gcc replacement at 67% (t3); and gcc replacement at 100% (t4), respectively. during the experiment, concentrate was offered at 0.5% bw while 5% urea-treated ... | 2012 | 25049671 |
| comparison of α1-antitrypsin, α1-acid glycoprotein, fibrinogen and nox as indicator of subclinical mastitis in riverine buffalo (bubalus bubalis). | mastitis set apart as clinical and sub clinical is a disease complex of dairy cattle, with sub clinical being the most important economically. of late, laboratories showed interest in developing biochemical markers to diagnose sub clinical mastitis (scm) in herds. many workers reported noteworthy alternation of acute phase proteins (apps) and nitric oxide, (measured as nitrate+nitrite = nox) in milk due to intra-mammary inflammation. but, the literature on validation of these parameters as indic ... | 2013 | 25049851 |
| effect of cattle breeds on milk composition and technological characteristics in china. | cattle breeds have a striking effect on milk, including milk composition and technological characteristics. this study aims to compare milk composition, acidification activity, viscosity, milk dispersion system stability and casein molecular weight among three buffalo breeds in china. the technological characteristics of milk produced by three cattle breeds of river buffalo (murrah), crossbreed 1st generation (f1), crossbreed multiple generation (fh, h≥3) buffaloes were investigated. cattle bree ... | 2013 | 25049865 |
| carcass characteristics and meat quality of swamp buffaloes (bubalus bubalis) fattened at different feeding intensities. | twenty-four male 1-year old swamp buffaloes (bubalus bubalis) were randomly allocated to 4 groups. one group grazed on guinea grass (gg) and another on guinea grass and the legume stylosanthes guianensis (gl). the other two groups were kept in pens and fed freshly cut guinea grass and concentrate at an amount of 1.5% (gc1.5) and 2.0% (gc2.0) of body weight, respectively. the effect of the different feeding intensities on carcass characteristics and meat quality were assessed. the mean body weigh ... | 2014 | 25049987 |
| cloning and sequence analysis of wild argali isg15 cdna. | the complete coding sequence of wild argali isg15 cdna was generated by rapid amplification of cdna ends. the isg15 cdna was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kda protein composed of 157 amino acids. its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of isg15cdna from ovis aries (accession no. nm001009735.1), capra hircus (accession no. hq329186.1), bos taurus (accession no. bc102318.1), bubalus bubalis (accession no. hm543269.1) ... | 0 | 25049988 |
| prevalence and antimicrobial susceptibility of arcobacter species in cow milk, water buffalo milk and fresh village cheese. | in this study, the presence of arcobacter spp. was examined in cow milk (n=50), water buffalo (wb) milk (n=50) and fresh village cheese (n=50) samples. the 16s rdna-rflp method was used for the identification of arcobacter spp. the disc diffusion method was used to investigate the susceptibility of all strains identified to 18 different antimicrobial substances. the most commonly isolated arcobacter species were found to be arcobacter butzleri (38.89%), arcobacter cryaerophilus (22.23%) and arco ... | 2014 | 25064812 |
| karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed. | domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. in order to have a better characterization of these different cytotypes of buffaloes, and considering that nor-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (ag-nor) and perfo ... | 2014 | 25071402 |
| mapping whole genome shotgun sequence and variant calling in mammalian species without their reference genomes. | genomics research in mammals has produced reference genome sequences that are essential for identifying variation associated with disease. high quality reference genome sequences are now available for humans, model species, and economically important agricultural animals. comparisons between these species have provided unique insights into mammalian gene function. however, the number of species with reference genomes is small compared to those needed for studying molecular evolutionary relati ... | 2013 | 25075278 |
| efficiency to reach age of puberty and behaviour of buffalo heifers (bubalus bubalis) kept on pasture or in confinement. | in order to evaluate the influence of rearing system (free-ranging (fr) v. confinement (c)) on buffalo heifer efficiency to reach age of puberty and on behavioural and immune functions, two experiments were conducted from september 2010 to october 2011. in experiment i, 32 subjects aged 8 to 9 months at the start of experiment were used. a total of 16 animals (group c) were group housed in an indoor slatted floor pen (4 m2/animal) with an outdoor paddock (4 m2/animal); 16 others grazed on a medi ... | 2014 | 25076110 |
| quantitative expression of pluripotency-related genes in parthenogenetically produced buffalo (bubalus bubalis) embryos and in putative embryonic stem cells derived from them. | parthenogenetically produced embryos and embryonic stem (es) cells derived from them offer a unique model for investigating the role of transcription factors in embryonic genome activation (ega), pluripotent lineage specification and in pluripotency and self-renewal of es cells because of the unique nature of these embryos. there is little information on the quantitative expression of important genes in parthenogenetically produced embryos and in es cells derived from them. the present study exa ... | 2014 | 25077841 |
| differential expression of homeobox c11 protein in water buffalo bubalus bubalis and its putative 3d structure. | the homeobox (hox) family complex contains 39 genes, clustered into four groups (a-d) all expressing in sequential manner. the hox proteins are transcriptional factors involved in regulation of pattern formation of the anterio-posterior body axis across the species. most of the hox family genes have been studied with respect to their organization and expression during the embryonic stages. however, expression pattern of homeobox c11 (hoxc11) gene in the 5' region, particularly in higher mammals ... | 2014 | 25080327 |
| draft genome sequence of pasteurella multocida subsp. multocida b:2 strain vtccbaa264 isolated from bubalus bubalis in north india. | the pasteurella multocida subsp. multocida b:2 serotype causes hemorrhagic septicemia in bubalines with high morbidity and mortality in the indian subcontinent. we report the draft genome sequence of pasteurella multocida strain vtccbaa264 isolated from the small-intestine of a buffalo calf that died of high fever. | 2014 | 25081265 |
| redescription of sarcocystis fusiformis sarcocysts from the water buffalo (bubalus bubalis). | four valid species of sarcocystis have been reported from the water buffalo (bubalus bubalis): sarcocystis fusiformis, sarcocystis buffalonis, sarcocystis levinei and sarcocystis dubeyi. here, we redescribe structure of s. fusiformis sarcocysts by scanning and transmission electron microscopy (sem, tem). twenty-one macroscopic sarcocysts from oesophagus of the water buffalo in egypt were examined by light microscopy, sem and tem. the sarcocyst wall was up to 9 μm thick, depending on the section ... | 2015 | 25111676 |
| in-vitro indicators of natural resistance and milk-producing ability in dairy buffaloes (bubalus bubalis). | the aim of this study was to explore the possibility of detecting novel phenotypes of natural resistance at the molecular level through the in-vitro stimulation of monocyte-derived macrophages (mdms). this study was conducted with 16 healthy buffaloes who were reared for milk production and for whom data on milk-producing ability were available for several lactations. mdms from circulating monocytes were activated with interferon-gamma and lipopolysaccharide. the response was evaluated using wes ... | 2014 | 25111879 |
| 'soya milk tris-based phytoextender reduces apoptosis in cryopreserved buffalo (bubalus bubalis) spermatozoa'. | the objective of this study was to investigate the effect of newly developed soya milk tris (smt)-based phytoextender as an alternative to egg yolk tris (eyt) extender used for cryopreservation of buffalo (bubalus bubalis) spermatozoa on apoptosis. fresh buffalo semen (control without dilution) was cryopreserved in conventional eyt (20% egg yolk v/v in tris) and smt (25% soya milk v/v in tris) extender and used for the assessment of expression of apoptotic proteins. proteins extracted from a tot ... | 2014 | 25112668 |
| a taxonomic review of the genus megalostomis chevrolat (coleoptera, cryptocephalinae, chrysomelidae). | a taxonomic revision of the genus megalostomis chevrolat, 1836 (coleoptera: chrysomelidae: cryptocephalinae: clytrini: megalostomina) is provided, including new data on geographic ranges. two new species and 34 new synonymies are proposed within the genus megalostomis, leaving 42 valid species in the genus. a checklist of the species of megalostomis is provided, with information on host plants, ant associations, and natural enemies. the study includes a key to all species, diagnoses, description ... | 2013 | 25113596 |
| pheno- and genotyping of brucella abortus biovar 5 isolated from a water buffalo (bubalus bubalis) fetus: first case reported in the americas. | an isolate of brucella spp. from an aborted water buffalo (bubalus bubalis) fetus was characterized based on its pheno- and genotype. the phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with brucella a and m monospecific antisera. the genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence is711 in the brucell ... | 2014 | 25113673 |
| a comparison of flotac and cff techniques in detecting gastrointestinal parasites in water buffaloes (bubalus bubalis). | the objective of the study was to compare the usefulness of flotac and centrifugal fecal flotation (cff) techniques. more specifically, the taxonomic classes (nematoda and cestoda) of endoparasites present in fecal samples of buffaloes are identified, the sensitivity and specificity of flotac relative to cff are calculated, and the agreement of both techniques is evaluated using kappa statistics. fresh fecal samples from 220 buffaloes in 10 municipalities were collected. sheather's sugar was use ... | 2014 | 25115063 |
| probiotic properties of lactic acid bacteria isolated from water-buffalo mozzarella cheese. | this study evaluated the probiotic properties (stability at different ph values and bile salt concentration, auto-aggregation and co-aggregation, survival in the presence of antibiotics and commercial drugs, study of β-galactosidase production, evaluation of the presence of genes encoding mapa and mub adhesion proteins and ef-tu elongation factor, and the presence of genes encoding virulence factor) of four lab strains (lactobacillus casei sjrp35, leuconostoc citreum sjrp44, lactobacillus delbru ... | 2014 | 25117002 |
| molecular characterization of oxytocin receptor gene in water buffalo (bubalus bubalis). | buffaloes are known for their productivity as compared to average yielding cows due to higher fat percentage, better feed conversion ability and disease resistance. on the other hand, the reproductive performances of buffaloes are often considered as poor owing to late sexual maturity, weak/silent oestrus, repeat breeder and prolonged intercalving interval. the study of cascade of events during oestrus and oestrous cycle can be useful for the improvement of reproductive efficiency of buffaloes. ... | 2014 | 25132183 |
| role of linker histone h1c during the reprogramming of chinese swamp buffalo (bubalus bubalis) embryos produced by somatic cell nuclear transfer. | during reprogramming, there is exchange of histone h1c and the oocyte-specific linker histone, and h1c may play a critically important role in the reprogramming process of somatic cell nuclear transfer (scnt). the aim of the present study was to investigate the role of the h1c gene in scnt reprogramming in chinese swamp buffalo (bubalus bubalis) using rna interference (rnai). chinese swamp buffalo h1c gene sequences were obtained and h1c-rnai vectors were designed, synthesised and then transfect ... | 2016 | 25145348 |
| comparison of endocrine changes, timing of ovulations, ovarian follicular growth, and efficacy associated with estradoublesynch and heatsynch protocols in murrah buffalo cows (bubalus bubalis). | poor estrus expression and the difficulty encountered in predicting the time of ovulation compromise the reproductive efficiency of murrah buffalo cows. synchronization of ovulation and timed artificial insemination are able to precisely control the time of ovulation and thus avoid the need for estrus detection. recently, the estradoublesynch protocol (administration of a pgf2α injection 2 days before heatsynch protocol; gnrh 0, pgf2α 7, estradiol benzoate [eb] 8) was developed that precisely sy ... | 2014 | 25145622 |
| a comparative protein profile of mammalian erythrocyte membranes identified by mass spectrometry. | a comparative analysis of erythrocyte membrane proteins of economically important animals, goat (capra aegagrus hircus), buffalo (bubalus bubalis), pig (sus scrofa), cow (bos tauras), and human (homo sapiens) was performed. solubilized erythrocyte membrane proteins were separated by sodium dodecyl sulfate-polyacryamide gel electrophoresis (sds-page), visualized by staining the gels with commassie brilliant blue (cbb), and identified by matrix assisted laser desorption ionization time-of-flight m ... | 2014 | 25150706 |
| seasonal variations in developmental competence and relative abundance of gene transcripts in buffalo (bubalus bubalis) oocytes. | hot season is a major constraint to production and reproduction in buffaloes. the present work aimed to investigate the effect of season on ovarian function, developmental competence, and the relative abundance of gene expression in buffalo oocytes. three experiments were conducted. in experiment 1, pairs of buffalo ovaries were collected during cold season (cs, autumn and winter) and hot season (hs, spring and summer), and the number of antral follicles was recorded. cumulus oocyte complexes (c ... | 2014 | 25156970 |
| molecular investigation of foot-and-mouth disease virus in domestic bovids from gharbia, egypt. | an outbreak of foot-and-mouth disease (fmd) affecting cattle and water buffalo (bubalus bubalis) occurred in egypt during 2012/2013. the present study was undertaken to determine the current strains of the fmd virus (fmdv) and the prevalence of fmd among cattle and buffalo in gharbia, egypt. the diagnostic sensitivity of two rt-pcr assays for the detection of fmdv was evaluated. the results revealed that sat2 was the causative agent. the percentage of infected of animals varied with the detectio ... | 2014 | 25187028 |
| molecular cloning and characterization of babesia orientalis rhoptry-associated protein 1. | the rhoptry-associated protein 1 (rap-1) gene of babesia orientalis was obtained from a cdna expression library by immunoscreening with b. orientalis-infected water buffalo sera. the nucleotide sequence of the cdna was 1732 bp with an open reading frame (orf) of 1434 bp, encoding a polypeptide of 478 amino acid residues with a predicted size of 52.5 kda. the orf was cloned into a pgex-kg plasmid and subsequently expressed as a gst-fusion protein. the recombinant rap-1 of b. orientalis (rborap-1) ... | 2014 | 25199690 |
| infection of water buffalo in rio de janeiro brazil with anaplasma marginale strains also reported in cattle. | anaplasma marginale is the most prevalent pathogen of cattle in tropical and subtropical regions of the world and causes the disease bovine anaplasmosis. the importance of water buffalo in the world economy is increasing. in addition, while water buffalo may serve as a reservoir host for a. marginale, the susceptibility of this host for a. marginale cattle strains in brazil has not been reported. the major surface protein 1 alpha (msp1α) gene has been shown to be a stable genetic marker for iden ... | 2014 | 25260335 |
| seroprevalence of toxoplasma gondii infection in water buffaloes (bubalus bubalis) in veracruz state, mexico and its association with climatic factors. | infection with toxoplasma gondii in water buffaloes (bubalus bubalis) is of epidemiological importance because of the risk for transmission to humans. we sought to determine the seroprevalence of t. gondii infection in 339 water buffaloes in veracruz state, mexico using the modified agglutination test (mat, cut off 1:25). seroprevalence association with general characteristics of buffaloes and their environment was also investigated. | 2014 | 25267265 |
| morphology of the lingual papillae of egyptian buffalo (bubalus bubalis). | the mammalian tongue has different functions for feeding. we investigated the morphological characteristics of the surface of the tongue of egyptian buffalo (bubalus bubalis) using scanning electron microscopy. the lingual surface exhibited different degrees of specialization. the tongue can be divided into the apex, body, and root. the lingual prominence was observed on the posterior area of the lingual body. the lenticular, conical, and vallate papillae were observed on the caudal part of the ... | 2014 | 25274404 |
| anti-mullerian hormone concentration and antral ovarian follicle population in murrah heifers compared to holstein and gyr kept under the same management. | this study was performed to evaluate plasma concentrations of anti-mullerian hormone (amh) and the ovarian antral follicle population (afp) in different genetic groups. cyclic heifers (13 bubalus bubalis [murrah]; 15 bos taurus [holstein] and 10 bos indicus [gyr]) were maintained under the same management and were synchronized with two doses of 150 μg im d-cloprostenol administered 14 days apart. after the second d-cloprostenol treatment, heifers had their ovaries scanned daily by ultrasound to ... | 2014 | 25291989 |
| identification of pregnancy-associated glycoproteins by peptide mass fingerprinting in water buffalo (bubalus bubalis). | ruminant placentas synthesize pregnancy-associated glycoproteins (pags) during pregnancy, which serve as biomarkers of pregnancy. the present study was conducted to verify, whether pags are expressed in buffalo placenta by using lectin-based affinity chromatography and peptide mass finger printing (pmf). fetal cotyledonary tissues were collected from gravid uteri procured from slaughtered house. proteins were extracted and subjected to wheat germ agglutinin (wga) lectin affinity chromatography t ... | 2014 | 25296505 |
| expression and localization of insulin-like growth factor system in corpus luteum during different stages of estrous cycle in water buffaloes (bubalus bubalis) and the effect of insulin-like growth factor i on production of vascular endothelial growth factor and progesterone in luteal cells cultured in vitro. | this study investigated the expression and localization of insulin-like growth factor (igf) system at different stages of buffalo cl and the role of igf-i in stimulating vascular endothelial growth factor (vegf) and progesterone (p4) production in cultured luteal cells. the mrna expression of igf system, vegf, steroidogenic acute regulatory protein, p450scc, and hydroxysteroid dehydrogenase (hsd) was investigated by quantitative real-time polymerase chain reaction (pcr). protein expression of ig ... | 2015 | 25304995 |
| microscopical and serological studies on sarcocystis infection with first report of s. cruzi in buffaloes (bubalus bubalis) in assiut, egypt. | this study was performed for the purpose of investigating the prevalence and the species composition of sarcocystis spp. in buffaloes in assiut province, egypt. macroscopically we reported the infection of buffaloes with sarcocystis fusiformis, while microscopically three sarcocystis species (sarcocystis cruzi, sarcocystis levinei and sarcocystis hominis) cysts were recognized, and were differentiated by their morphological features using both histopathological sections and electron microscope s ... | 2014 | 25320487 |
| abortion and foetal lesions induced by neospora caninum in experimentally infected water buffalos (bubalus bubalis). | the water buffalo (bubalus bubalis) is an important species in several countries for its milk and meat production, as well as for transport and other agricultural activities. it is, in general, considered more resistant than cattle to different parasitic diseases, also less demanding for forage quality. it has been postulated that buffalo may be resistant to abortion caused by neosporosis, because of high serological prevalences found in buffalo herds from different localities, with no descripti ... | 2015 | 25324135 |
| sequential cross-species chromosome painting among river buffalo, cattle, sheep and goat: a useful tool for chromosome abnormalities diagnosis within the family bovidae. | the main goal of this study was to develop a comparative multi-colour zoo-fish on domestic ruminants metaphases using a combination of whole chromosome and sub-chromosomal painting probes obtained from the river buffalo species (bubalus bubalis, 2n = 50,xy). a total of 13 dna probes were obtained through chromosome microdissection and dop-pcr amplification, labelled with two fluorochromes and sequentially hybridized on river buffalo, cattle (bos taurus, 2n = 60,xy), sheep (ovis aries, 2n = 54,xy ... | 2014 | 25330006 |
| molecular cloning and expression analysis of the stat1 gene in the water buffalo (bubalus bubalis). | signal transducer and activator of transcription 1 (stat1) is a critical component of the transcription factor complex in the interferon (ifn) signaling pathways. of the seven stat isoforms, stat1 is a key mediator of type i and type iii ifn signaling, but limited information is available for the stat genes in the water buffalo. here, we amplified and identified the complete coding sequence (cds) of the buffalo stat1 gene by using reverse transcription polymerase chain reaction (rt-pcr). sequenc ... | 2015 | 25336386 |
| expression and localization of aquaporin-1 along the intestine of colostrum suckling buffalo calves. | aquaporin-1 (aqp1), a six-transmembrane domain protein, belongs to a highly conserved group of proteins called aquaporins known to regulate permeability across cell membranes. although the role of aqp1 has been extensively studied, its specific activity along the gastrointestinal tract in animals during early postnatal development is poorly known. this study investigates the expression of aqp1 mrna and protein in the small and large intestine of water buffalo calves after colostrum ingestion usi ... | 2015 | 25348329 |
| molecular characterization of various trichomonad species isolated from humans and related mammals in indonesia. | trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in indonesia and molecularly characterized. the dna sequences of the partial 18s small subunit ribosoma ... | 2014 | 25352694 |
| the effect of buffalo cd14 shrna on the gene expression of tlr4 signal pathway in buffalo monocyte/macrophages. | cd14 plays a crucial role in the inflammatory response to lipopolysaccharide (lps), which interacts with tlr4 and md-2 to enable cell activation, resulting in inflammation. upstream inhibition of the inflammation pathway mediated by bacterial lps, toll-like receptor 4 (tlr4) and cluster of differentiation antigen 14 (cd14) was proven to be an effective therapeutic approach for attenuating harmful immune activation. to explore the effect of cd14 downregulation on the expression of tlr4 signaling ... | 2014 | 25355240 |
| toll-like receptor responses to peste des petits ruminants virus in goats and water buffalo. | ovine rinderpest or goat plague is an economically important and contagious viral disease of sheep and goats, caused by the peste des petits ruminants virus (pprv). differences in susceptibility to goat plague among different breeds and water buffalo exist. the host innate immune system discriminates between pathogen associated molecular patterns and self antigens through surveillance receptors known as toll like receptors (tlr). we investigated the role of tlr and cytokines in differential susc ... | 2014 | 25369126 |
| assessment of sperm damages during different stages of cryopreservation in water buffalo by fluorescent probes. | the present study was designed to investigate the sperm damages occurring in acrosome, plasma membrane, mitochondrial activity, and dna of fresh, equilibrated and frozen-thawed buffalo semen by fluorescent probes. the stability of sperm acrosome and plasma membrane stability, mitochondrial activity and dna status were assessed by fluorescein conjugated lectin pisum sativum agglutinin, annexin-v/propidium iodide, jc-1 and tunel assay, respectively, under the fluorescent microscope. the damages pe ... | 2016 | 25373338 |
| effects of in vitro zinc sulphate additive to the semen extender on water buffalo (bubalusbubalis) spermatozoa before and after freezing. | the objective of the study was to investigate the effects of in vitro zinc sulphate additive to semen extender on sperm parameters (progressive motility, viability, membrane integrity and dna stability) after cryopreservation. | 2014 | 25379162 |
| expression of mrna encoding igf-i, igf-ii, type-i,and ii igf-receptors and igf-binding proteins-1-4 during ovarian follicular development in buffalo (bubalus bubalis). | the present study was designed to investigate the expression pattern of igf-i, igf-ii, type-i and ii igf-receptors, and igfbp-1-4 in different stages of buffalo ovarian preantral follicles (pfs), antral follicles (afs), ovulatory follicles (ofs), and immature (im) and in vitro matured (mo) oocytes. buffalo ovaries were collected from local abattoir, pfs (200-250 µm), afs (1-3 mm), and ofs (5-8 mm) were isolated by mechanical method. pfs, afs, ofs, and oocytes were lysed to release mrna, reverse ... | 2015 | 25380459 |
| assessment of genetic variability and structuring of riverine buffalo population (bubalus bubalis) of indo-gangetic basin. | the buffalo population of uttar pradesh (up) constitutes 26.1% of the total buffalo population of india, yet this population has not been classified into distinct breeds or subpopulations due to lack of systematic study. genetic variation at 30 microsatellite loci was examined and statistical analysis was carried out to reveal genetic diversity, demographic parameters of these buffaloes and to investigate the existence of population substructures underlying geographical distribution. the mean nu ... | 2015 | 25380468 |
| effect of additional chromium supplementation on health status, metabolic responses, and performance traits in periparturient murrah buffaloes (bubalus bubalis). | this study was conducted to determine the effects of inorganic chromium (cr) on body condition, metabolic responses, lactation performance, and reproductive parameters in periparturient murrah buffaloes. twenty-four multiparous advanced pregnant murrah buffaloes were randomly assigned to four treatment diets. feeding regimen was the same in all the groups, except that the animals in the four respective groups were additionally supplemented with 0.0, 0.5, 1.0, and 1.5 mg of cr/kg dry matter (dm) ... | 2015 | 25388756 |
| effect of dietary protein on intake, nutrients utilization, nitrogen balance, blood metabolites, growth and puberty in growing bhadawari buffalo (bubalus bubalis) heifers. | fifteen bhadawari buffalo heifers of 207 ± 9.78 kg mean body weight were randomly distributed into three dietary groups to evaluate the effect of protein level on nutrient utilization, nitrogen (n) balance, growth rate, blood metabolites, and puberty. all animals were offered wheat straw-berseem diets supplemented with concentrate mixtures of similar energy (2.7 mcal/kg) and different protein levels (14.3-22%). animals of standard-protein group (spg) were offered protein and energy as per requir ... | 2015 | 25407740 |
| treatment of buffalo (bubalus bubalis) donor cells with trichostatin a and 5-aza-2'-deoxycytidine alters their growth characteristics, gene expression and epigenetic status and improves the in vitro developmental competence, quality and epigenetic status of cloned embryos. | we examined the effects of treating buffalo skin fibroblast donor cells with trichostatin a (tsa), a histone deacetylase (hdac) inhibitor, and 5-aza-2'-deoxycytidine (5azadc), a dna methyltransferase (dnmt) inhibitor, on the cells and embryos produced by hand-made cloning. treatment of donor cells with tsa or 5azadc resulted in altered expression levels of the hdac1, dnmt1, dnmt3a, p53, caspase3 and caspase9 genes and global levels of acetylation of lysine at position 9 or 14 in histone 3 (h3k9/ ... | 2016 | 25409339 |
| comparison of pharmacokinetics of marbofloxacin after subcutaneous administration of various multiple-dose regimens to water buffalo calves (bubalus bubalis). | to determine pharmacokinetics of marbofloxacin in water buffalo calves (bubalus bubalis) after multiple sc administrations and to assess differences in regimen efficacy. | 2014 | 25419804 |
| detection of fraudulent addition of bovine whey in water buffalo ricotta cheese by isoelectric focusing. | prevention of food fraud in the dairy field is a difficult issue for researchers, industries and policy makers, both for commercial and health reasons. currently, no analytical method allows detection of the addition of bovine whey to water buffalo ricotta, so this fraudulent practice cannot be prevented. the authors' aim was to develop such a method. | 2015 | 25425399 |
| characterization of leptin receptor gene in bubalus bubalis and association analysis with body measurement traits. | leptin has a pleiotropic effect on regulating appetite, energy metabolism, growth, reproduction, body composition and immunity. this property supports leptin and its receptor as candidate genes for evaluating genetic polymorphisms to associate with growth, milk yield and other economic traits. the aim of this study is to characterize the leptin receptor gene in bubalus bubalis, to identify single-nucleotide polymorphism (snp) sites in different coding and non-coding regions and to analyse potent ... | 2015 | 25431006 |
| igf-1 attenuates lps induced pro-inflammatory cytokines expression in buffalo (bubalus bubalis) granulosa cells. | interaction between immune and endocrine system is a diverse process influencing cellular function and homeostasis in animals. negative energy balance (neb) during postpartum period in dairy animals usually suppresses these systems resulting in reproductive tract infection and infertility. these negative effects could be due to competition among endocrine and immune signaling pathways for common signaling molecules. the present work studied the effect of igf-1 (50 ng/ml) on lps (1 μg/ml) mediate ... | 2015 | 25433435 |
| the interval between the emergence of pharmacologically synchronized ovarian follicular waves and ovum pickup does not significantly affect in vitro embryo production in bos indicus, bos taurus, and bubalus bubalis. | the aim of the present study was to determine the optimal phase of the follicular wave to perform ovum pickup (opu) for in vitro embryo production (ivep) in various genetic groups. for this purpose, 27 heifers-nine bos taurus (holstein), nine bos indicus (nelore), and nine bubalus bubalis (mediterranean)-were maintained under the same nutritional, management, and environmental conditions. heifers within each genetic group were submitted to six consecutive opu trials with 14-day intersession inte ... | 2015 | 25447149 |
| temporal leakage of cu,zn superoxide dismutase and loss of two low-molecular-weight forms of glutathione peroxidase-1 from buffalo (bubalus bubalis) sperm after freezing and thawing. | the postthaw motility and fertility of frozen-thawed buffalo spermatozoa are substantially low as compared with those of cattle sperm. the sperm motility and fertility have been positively correlated with the antioxidant enzyme activities of human and canine sperm. however, the extent of antioxidant enzyme loss during cryopreservation, although reported for human and cattle sperm, is still not clear for buffalo sperm. thus, in the present study, an attempt was made to determine the activities of ... | 2015 | 25459023 |
| clinical outcomes and molecular genotyping of staphylococcus aureus isolated from milk samples of dairy primiparous mediterranean buffaloes (bubalus bubalis). | staphylococcus aureus is one of the most important pathogens causing mastitis in dairy cows and in mediterranean buffaloes. genotype b (gtb) is contagious in dairy cows and may occur in up to 87% of cows of a dairy herd. it was the aim of this study to evaluate genotypes present, clinical outcomes, and prevalence of staph. aureus in milk samples of primiparous mediterranean dairy buffaloes. two hundred composite milk samples originating from 40 primiparous buffaloes were collected from may to ju ... | 2014 | 25459906 |
| comparative efficacies of six different media for cryopreservation of immature buffalo (bubalus bubalis) calf testis. | buffalo calves have a high mortality rate (~80%) in commercial dairies and testis cryopreservation can provide a feasible option for the preservation of germplasm from immature males that die before attaining sexual maturity. the aim of the present study was to evaluate combinations of 10 or 20% dimethylsulfoxide (dmso) with 0, 20 or 80% fetal bovine serum (fbs) for cryopreservation of immature buffalo testicular tissues, subjected to uncontrolled slow freezing. tissues cryopreserved in 20% dmso ... | 2014 | 25482277 |
| comparative expression profiling of insulin-like growth factor binding protein-5 in milk of bos indicus and bubalus bubalis during lactation. | insulin-like growth factor binding protein-5 (igfbp-5) is a key molecule in mammary gland development, which facilitates the removal of mammary epithelial cells (mecs) by apoptosis that takes place during remodeling of the mammary gland during involution. igfbp-5 binds with igfs for their bioavailability. igfbp-5 has been reported to perform pleiotropic roles such as cellular apoptosis, proliferation and differentiation. to understand the role of igfbp-5 during lactation and clinical mastitis, e ... | 2015 | 25491373 |
| identification of single nucleotide polymorphisms in toll-like receptor candidate genes associated with tuberculosis infection in water buffalo (bubalus bubalis). | toll-like receptors play a key role in innate immunity by recognizing pathogens and activating appropriate responses. pathogens express several signal molecules (pathogen-associated molecular patterns, pamps) essential for survival and pathogenicity. recognition of pamps triggers an array of anti-microbial immune responses through the induction of various inflammatory cytokines. the objective of this work was to perform a case-control study to characterize the distribution of polymorphisms in th ... | 2014 | 25496717 |
| seroprevalence and risk factors associated with exposure of water buffalo (bubalus bubalis) to neospora caninum in northeast thailand. | water buffalo are important draft animals for agriculture in resource-restricted areas worldwide. water buffalo were shown to be experimentally susceptible to infection with neospora caninum, potentially affected by neosporosis, and naturally exposed to the parasite in asia. although enzootic to thailand, the distribution of n. caninum among thai water buffalo is unclear. the objectives of this study were to determine the seroprevalence of n. caninum among water buffalo of northeast thailand and ... | 2015 | 25499824 |
| comparative analysis of the river buffalo somatostatin gene. | the somatostatin protein plays a crucial role in the regulation of multiple biological functions, such as growth, fat deposition, and nutrient absorption in vertebrates. polymorphisms in the somatostatin gene have been associated with growth traits in livestock species, including cattle and goat. in this study, we conducted complete molecular characterization of the somatostatin gene in bubalus bubalis (murrah breed) by sequencing a murrah bac clone spanning 72,489 base pairs (bp) in length. the ... | 2014 | 25501213 |
| a comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (bubalus bubalis) embryos. | the efficiency of two cell types, namely adult fibroblasts, and amniotic fluid stem (afs) cells as nuclear donor cells for somatic cell nuclear transfer by hand-made cloning in buffalo (bubalus bubalis) was compared. the in vitro expanded buffalo adult fibroblast cells showed a typical "s" shape growth curve with a doubling time of 40.8 h and stained positive for vimentin. the in vitro cultured undifferentiated afs cells showed a doubling time of 33.2 h and stained positive for alkaline phosphat ... | 2016 | 25501536 |
| differential histone modification status of spermatozoa in relation to fertility of buffalo bulls. | in this study genome-wide di-methylated h3k4 (h3k4me2) and tri-methylated h3k27 (h3k27me3) modification profiles were analyzed in spermatozoa of buffalo bulls having wide fertility differences. the custom designed 4 × 180 k buffalo (bubalus bubalis) chip-on-chip array was fabricated by employing array-based sequential hybridization using bovine and buffalo genomic dna for comparative hybridization. the buffalo specific array developed had 177,440 features assembled from coding sequences, promote ... | 2015 | 25501625 |
| sequence characterization and comparative analysis of the gastrotropin gene in buffalo (bubalus bubalis). | in this study, we compared the complete sequence of the fabp6 gene from an animal representing the murrah breed of the river buffalo (bubalus bubalis) with the gene sequence from different mammals. the buffalo fabp6 gene is 6105 bp in length and is organized into four exons (67, 176, 90, and 54 bp), three introns (1167, 1737, and 2649 bp), a 5ꞌutr (93 bp), and a 3ꞌutr (72 bp). a total of 22 repetitive elements were identified at the intronic level, and four of these (l1mc, l1m5, mirb, and charli ... | 2014 | 25526214 |
| production technology and characterization of fior di latte cheeses made from sheep and goat milks. | innovation in the small ruminant dairy sector faces structural challenges because dairies are often involved in breeding and produce cheeses that appeal essentially to local markets using traditional technologies and facilities. an investigation was carried out to produce fior di latte, a traditional, soft pasta filata cheese, from sheep and goat milks at the farm level. fior di latte is an italian high-moisture, round mozzarella currently produced from cow and water buffalo milks; it is very po ... | 2015 | 25547300 |
| genetic diversity in trypanosoma theileri from sri lankan cattle and water buffaloes. | trypanosoma theileri is a hemoprotozoan parasite that infects various ruminant species. we investigated the epidemiology of this parasite among cattle and water buffalo populations bred in sri lanka, using a diagnostic pcr assay based on the cathepsin l-like protein (catl) gene. blood dna samples sourced from cattle (n=316) and water buffaloes (n=320) bred in different geographical areas of sri lanka were pcr screened for t. theileri. parasite dna was detected in cattle and water buffaloes alike ... | 2015 | 25554063 |
| reproductive characteristics and thyroidal function in relation with season in khuzestan buffalo (bubalus bubalis) bulls. | high ambient temperature is the major constraint on buffalo productivity. the aim of this study was to evaluate the reproductive performance and thyroid gland function in winter and summer seasons in khuzestan buffalo bulls. six male indigenous buffaloes of khuzestan with nearly the same age (2-3 years old) and weight were used. semen and blood samples through jugular vein were collected, every two weeks throughout the summer and winter seasons. the thyroid hormones and thyrotropin stimulating h ... | 2014 | 25568719 |
| acui identifies water buffalo csn3 genotypes by rflp analysis. | 2014 | 25572073 | |
| genetic variations in merozoite surface antigen genes of babesia bovis detected in vietnamese cattle and water buffaloes. | the genes that encode merozoite surface antigens (msas) in babesia bovis are genetically diverse. in this study, we analyzed the genetic diversity of b. bovis msa-1, msa-2b, and msa-2c genes in vietnamese cattle and water buffaloes. blood dna samples from 258 cattle and 49 water buffaloes reared in the thua thien hue province of vietnam were screened with a b. bovis-specific diagnostic pcr assay. the b. bovis-positive dna samples (23 cattle and 16 water buffaloes) were then subjected to pcr assa ... | 2015 | 25575442 |
| the in vitro effect of leptin on semen quality of water buffalo (bubalus bubalis) bulls. | the purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and dna damage). semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (contro ... | 2013 | 25593679 |
| effects of in vitro copper sulphate supplementation on the ejaculated sperm characteristics in water buffaloes (bubalus bubalis). | this study was carried out to investigate effects of copper sulphate (cuso4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and dna damage, after semen dilution and cryopreservation. semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. a total number of 25 samples were used in each examination. sperm progressive motility and viability were measured at 0 (t0), 60 (t1) and 120 (t2) mi ... | 2013 | 25593683 |
| large-scale serological survey of bovine ephemeral fever in china. | bovine ephemeral fever (bef) is caused by the arthropod-borne bovine ephemeral fever virus (befv), which is classified in the family rhabdoviridae and the genus ephemerovirus. a debilitating and sometimes fatal viral disease, bef affects cattle and water buffalo. the epizootiology of bef among cattle in china has not been fully determined. we examined the seroprevalence of the befv among cattle in china between january 2012 and june 2014. among the 2822 serum samples collected from various cattl ... | 2015 | 25595266 |
| diversity of lactic acid bacteria isolated from brazilian water buffalo mozzarella cheese. | the water buffalo mozzarella cheese is a typical italian cheese which has been introduced in the thriving brazilian market in the last 10 y, with good acceptance by its consumers. lactic acid bacteria (lab) play an important role in the technological and sensory quality of mozzarella cheese. in this study, the aim was to evaluate the diversity of the autochthones viable lab isolated from water buffalo mozzarella cheese under storage. samples were collected in 3 independent trials in a dairy indu ... | 2015 | 25597646 |
| early cleavage of handmade cloned buffalo (bubalus bubalis) embryos is an indicator of their developmental competence and quality. | following ivf, embryos which cleave early have been shown to have higher developmental competence and quality than those that cleave relatively later across many species. we investigated the effect of time of cleavage on the developmental competence, quality, epigenetic status and gene expression in buffalo embryos produced by handmade cloning (hmc). following classification of embryos as early cleaving (ec) or late cleaving (lc) based on whether they had cleaved or not at 24 h post in vitro cul ... | 2015 | 25604613 |
| study of rumen metagenome community using qpcr under different diets. | the aim of this study was to detect the major bacteria present in rumen microbiota. here, we performed qpcr based absolute quantitation of selected rumen microbes in rumen fluid of river buffalo adapted to varying proportion of concentrate to roughage diets. animals were adapted to roughage-to-concentrate ratio in the proportion of 100:00 (t1), 75:25 (t2), 50:50 (t3) and 25:75 (t4) respectively for 30 days. at the end of each treatment, rumen fluid was collected at 0 h and 2 h after feeding. it ... | 2014 | 25606402 |