Publications

color graphics representations of large sequences in the gem environment.	the analytical and descriptive color graphics capabilities (the gem environment) of the cage/gem(a) software system are described using bacteriophage lambda (48,502 base pairs) and epstein barr virus (172,282 base pairs) as examples. genetics and features, as graphic drawings, and the results of sequence analysis as pseudo-colored representations, are simultaneously displayed at all zooming levels. the present upper limit on sequence size is 5 x 10(5) bases. the overlay editor utility which prov ...	1988	2832825
illegitimate recombination mediated by calf thymus dna topoisomerase ii in vitro.	we have found that purified calf thymus dna topoisomerase ii mediates recombination between two phage lambda dna molecules in an in vitro system. the enzyme mainly produced a linear monomer recombinant dna that can be packaged in vitro. novobiocin and anti-calf thymus dna topoisomerase ii antibody inhibit this atp-dependent recombination. the recombinant molecules contain duplications or deletions, and most crossovers take place between nonhomologous sequences of lambda dna, as judged by the seq ...	1988	2832845
[cloning of the dna bglii fragments of bacteriophage t4 in the vector plasmid pscc31].	an attempt has been made to clone six bglii fragments of t4 dna in the range of 3.3-8.1 kb in the vector plasmid pscc31 containing a single bglii site within the gene for endonuclease ecori and pl promoter of phage lambda. dna fragments were extracted from the corresponding bands of agarose gel. the following bglii fragments were cloned: the 3.3 kb fragment no. 9 containing a portion of gene 20, the gene 21 and a portion of gene 22; the 4.2 kb fragment no. 8.1 with genes 17, 18, 19 and a portion ...	1988	2833423
periodic interactions of heat shock transcriptional elements.	the activity of some genes is known to be a periodic function of the amount of dna between the binding sites of its regulatory proteins. the period observed is close to 10.5 base pairs (bp), that is, one turn of the b form of the dna helix. such periodicity is also seen in the cooperative binding of phage lambda and lac repressors. these periodic phenomena have been attributed to a requirement for a unique rotational alignment in forming essential contacts between the bound proteins. here we rep ...	1988	2833709
a simple and efficient procedure for the isolation of high-quality phage lambda dna using a deae-cellulose column.	a simple and rapid procedure for purifying large quantities of bacteriophage lambda particles and dna is described. the procedure involves deae-cellulose column chromatography of the phage particles and elution of the phage particles from the column with a low-ionic-strength buffer. the resulting phage were well separated from rna, dna, and proteins derived from escherichia coli host cells. the lambda dna was prepared from the purified phage particles by the conventional method of phenol extract ...	1988	2834980
identification and isolation of a putative permease gene in the quinic acid utilization (qut) gene cluster of aspergillus nidulans.	mutations in the qutd gene of aspergillus nidulans cause the loss of ability to grow upon quinic acid as sole carbon source in media at normal ph 6.5 and failure to induce three enzyme activities specifically required for metabolism to protochatechuic acid. all 9 qutd mutants recovered are recessive and have been found to be ph sensitive, growing strongly on quinic acid media at ph 3.5 and producing significant induced enzyme activities. these properties are consistent with the hypothesis that t ...	1987	2835177
neurospora crassa nuclear genome contains analogy of saccharomyces cerevisiae genes for ribosomal rna processing.	neurospora crassa wild type genome shows dna sequences which are homologous to the sequences present in the rrna processing genes of the yeast saccharomyces cerevisiae. five such processing genes from yeast, viz., rna1 through rna5, cloned in plasmid pbr322 were transformed in escherichia coli strain le392. southern blots containing dnas from these clones were restricted with several restriction endonucleases along with dnas from lambda phage, rice (plant) and neuroblastoma (animal), were hybrid ...	1987	2835180
terminase host factor: a histone-like e. coli protein which can bind to the cos region of bacteriophage lambda dna.	terminase host factor (thf), an e. coli protein capable of fulfilling the host factor requirement for in vitro bacteriophage lambda terminase activity, displays properties characteristic of the prokaryotic type ii dna-binding or "histone-like" proteins. it is a 22 k basic, heat- and acid-stable protein which binds non-specifically to various dnas. conditions can be established, however, where thf binds preferentially to the cohesive end site (cos) of lambda dna forming several distinct complexes ...	1988	2835746
chloroplast dna differences in the genus oenothera subsection munzia: a short direct repeat resembling the lambda chromosomal attachment site occurs as a deletion/insertion within an intron of an nadh-dehydrogenase gene.	a small restriction fragment length mutation has been mapped in the large inverted repeats of the chloroplast (cp) dna of munzia-oenothera species (vom stein and hachtel 1986). this mutation could be localized within the intron of a reading frame presumably coding for subunit b of an nadh-dehydrogenase (ndhb). sequence analysis revealed a 24 bp duplication/deletion. the predicted secondary structure of the ndhb-intron is altered by this duplication/deletion. part of the directly repeated segment ...	1988	2836087
fur (ferric uptake regulation) protein and cap (catabolite-activator protein) modulate transcription of fur gene in escherichia coli.	a fusion between the fur (ferric uptake regulation) gene, known to mediate negative regulation of iron absorption in escherichia coli, and lacz was constructed in vitro. beta-galactosidase levels of cells harboring this fusion were under the control of sequences contained in a 185-bp dna fragment located upstream of the fur structural gene. the fusion was prepared in multicopy (pvln102 plasmid) and low-copy-number states, the latter constructed as a lambda phage lysogen carrying a fur'-'lacz ins ...	1988	2836193
the cloning, dna sequence, and overexpression of the gene arae coding for arabinose-proton symport in escherichia coli k12.	a lambda placmu1 insertion was made into arae, the gene for arabinose-proton symport in escherichia coli. a phage containing an arae'-'lacz fusion was recovered from the lysogen and its restriction map compared with that of the 61-min region of the e. coli genome to establish the gene order thya arae orf lysr lysa galr; arae was transcribed toward orf. a 4.8-kilobase sali-ecori dna fragment containing arae was subcloned from the phage lambda d(lysa+ galr+ arae+) into the plasmid vector pbr322. f ...	1988	2836407
a point mutation in the nul gene of bacteriophage lambda facilitates phage growth in escherichia coli with hima and gyrb mutations.	a mutant of lambda was isolated that grows in the escherichia coli hima delta/gyrb-him320(ts) double mutant at 42 degrees c; conditions which are non-permissive for wild-type lambda growth. the responsible mutation, ohm1, alters the 40th codon of the nul reading frame. the nul and a gene products comprise the terminase protein which cleaves concatameric dna into unit-length phage genomes during dna packaging. the nul-ohm1 gene product acts in trans to support lambda growth in the double hima/gyr ...	1988	2836702
the bacteriophage lambda cohesive end site: isolation of spacing/substitution mutations that result in dependence on escherichia coli integration host factor.	substitution, insertion and deletion mutations have been constructed at the xmni restriction site in cos lambda. the xmni site is located between cosb, the site where terminase binds lambda dna; and cosn, the site where terminase introduces staggered nicks to generate cohesive ends. substitution mutations and deletion of a base pair (a -1 change) do not obviously affect lambda growth and dna packaging. changes of -2, +2 and -3 render lambda unable to grow on host cells lacking integration host f ...	1988	2836703
[alleviation of type i restriction in the presence of plasmids of group inc i. general characteristics and molecular cloning of the ard gene].	the capability of a number of plasmids of incn and inci groups to alleviate an action of type i ecok, ecob, ecod, and ecoa restriction endonucleases on the unmodified dna was revealed. the efficiency of ecok action on lambda 0 dna is alleviated about 10 divided by 100 fold in e. coli k12 ab 1157 bacteria containing the plasmid of incn group (pkm101, n3, pja4733) or inci group (r144, r648; r621a; colib-p9). we have cloned ard gene of colib-p9 plasmid (sali-c fragment) in pbr322 multicopying vecto ...	1988	2836721
[identification in vivo of promoter activity in the left site of the att region of bacteriophage lambda dna].	the promoter-probing vector (psk plasmid) was explored for cloning of the fragments from lambda ci857 and lambda b2 dnas containing different regions of the att site. we have constructed all-tet fusions where the fusions are: 1) hindiii/bamhi-491 base pairs (b. p.) fragment of lambda ci857 dna containing pop' site (plasmid psk-pp'); 2) alui-242 b. p. fragment of lambda ci857 dna containing the left arm of the pop' site (plasmid psk-p); 3) alui-242 b. p. fragment of lambda ci857 dna with opposite ...	1988	2836722
inhibition of dna restriction enzyme digestion by anthracyclines.	the inhibition of restriction enzyme digestion of lambda phage dna by anthracyclines (i.e., adriamycin, daunomycin, epirubicin, idarubicin and esorubicin) commonly used in the treatment of human leukemia and cancer has been studied in vitro. the anthracyclines used inhibit dna digestion by smai, avaii, haeiii, hhai and hpaii, which cut dna at guanine-cytosine (g-c) sequence sites, and by ecori, which cut dna at adenine adenine-thymine thymine (aatt) sequence sites. adriamycin completely inhibits ...	1988	2836942
assignment of the functional gene for human adrenodoxin to chromosome 11q13----qter and of adrenodoxin pseudogenes to chromosome 20cen----q13.1.	adrenodoxin is a small iron/sulfur protein serving as an electron-transport intermediate for all mitochondrial forms of cytochrome p450. southern blots of normal genomic dna cleaved with six restriction endonucleases probed with full-length human adrenodoxin cdna revealed complex patterns indicating the presence of multiple adrenodoxin genes. southern blots of dna from a panel of mouse/human somatic cell hybrids identified cross-hybridizing adrenodoxin dna in two loci, chromosome 11q13----qter a ...	1988	2837084
rglb facilitated cloning of highly methylated eukaryotic dna: the human l1 transposon, plant dna, and dna methylated in vitro with human dna methyltransferase.	in vitro methylation of bluescribe plasmid dna (pbs) with human placental dna methyltransferase to 6% 5-methylcytosine (mc) reduced transformation efficiencies in rglb+ host strains c600 and ds410 by almost 2 orders of magnitude. by contrast, the rglb- derivative of ds410 showed no reduction in transformation efficiency with methylation while the rglb- derivative of c600 was partially tolerant to methylation. further, we show that the 1.8 kilobase (kb) and 1.2 kb kpni fragments derived from the ...	1988	2837736
stage and strain specific expression of the tandemly repeated 90 kda surface antigen gene family in trypanosoma cruzi.	a recombinant cdna library constructed in the expression vector lambda gtll using mrna from the trypomastigote stage of trypanosoma cruzi was screened with two monoclonal antibodies that have been shown to react with a 105 kda and a 90 kda surface antigen in trypomastigotes of the peru and y strains of t. cruzi. one recombinant lambda phage, designated tcc-20, was reactive to both monoclonals. the beta-galactosidase/t. cruzi hybrid protein encoded in tcc-20 is recognized by the monoclonal antibo ...	1988	2838752
herpes simplex virus-directed overreplication of chromosomal dna physically linked to the simian virus 40 integration site of a transformed hamster cell line.	in the simian virus 40 (sv40)-transformed hamster cell line elona herpes simplex virus (hsv) induces amplification of sv40 dna sequences to high-molecular-weight head-to-tail concatemers indicating an extrachromosomal rolling circle replication. in order to enable investigations concerning intrachromosomal amplification of sv40 dna sequences and flanking cellular sequences a genomic library of elona dna was constructed in phage lambda. clones harboring cellular dna adjacent to the sv40 integrati ...	1988	2838966
abolition of dna recognition site resistance to the restriction endonuclease ecorii.	the ecorii recognition sites 5'-ccatgg-3' which occur three times in t3 dna are refractory to this restriction endonuclease. however, these sites are specifically cleaved by ecorii when a second, susceptible dna species (pbr 322, phage lambda) is present.	1988	2839163
purification and properties of the nusb protein of escherichia coli.	mutations in the nusb gene of escherichia coli block transcriptional antitermination mediated by the n gene protein of bacteriophage lambda. we describe here two methods of overproducing the nusb protein in e. coli and a method of purifying nusb to apparent homogeneity on a large scale. purified nusb directly stimulates transcriptional antitermination by the lambda n protein in vitro. it behaves as a monomer (mr = 15,689) during gel permeation chromatography and gradient sedimentation. the numbe ...	1988	2839479
the structure of the human skin fibroblast collagenase gene.	genomic clones containing the complete gene encoding human fibroblast interstitial collagenase were isolated from a lambda phage human dna library. the gene is comprised from 10 exons and spans 8.2 kilobase pairs. we have mapped the relative positions and determined the dna sequence of all the exon/intron borders of the gene. the organization of the human interstitial collagenase gene is very similar to that of rabbit collagenase and of two other extracellular matrix (ecm) metalloproteases: rat ...	1988	2839503
sequences in the 5' proximal segment of the paused transcript affect nusa-mediated enhancement of transcriptional pausing.	nusa protein is a transcription elongation and termination factor that acts to enhance pausing of rna chain growth by rna polymerase at specific sites on dna templates. we demonstrate that this enhancement of pausing in tr1, the transcription termination site between genes cro and cii of phage lambda, is inhibited by dna oligonucleotides complementary to a segment of the nascent rna just preceding the sequence that is thought to be a part of the stem of an rna hairpin that is responsible for pau ...	1988	2839506
molecular cloning and restriction enzyme mapping of an african swine fever virus isolate from malawi.	dna prepared from a field isolate of african swine fever virus, which causes high mortality and severe disease in domestic pigs, was cloned in bacteriophage lambda and plasmid vectors. clones containing dna inserts overlapping with each other and together covering the complete genome, apart from short fragments close to the cross-linked termini of the genome, were obtained. a complete restriction enzyme site map of the genome for three enzymes was deduced.	1988	2839602
action of gamma endonuclease on clustered lesions in irradiated dna.	irradiation of dna in situ i.e. in phage particles or in the cell leads to alterations of single dna nucleotides as well as to clustered lesions such as double strand breaks or unpaired dna regions the latter being sensitive to digestion by s1 nuclease. a contribution will be made to the configuration of such s1-nuclease-sensitive sites (s1 sites). dna from irradiated lambda phage containing s1 sites was treated with gamma endonuclease from m. luteus which is known to split the nucleotide strand ...	1988	2839862
cellular factors required for multiple stages of sv40 dna replication in vitro.	plasmids containing the sv40 origin replicate in the presence of sv40 t antigen and a cell free extract derived from human 293 cells. upon fractionation of this extract, two essential replication factors have been identified. one of these is a multi-subunit dna binding protein containing polypeptides of 70,000, 34,000 and 11,000 daltons which may function as a eukaryotic single strand dna binding protein (ssb). the other partially purified fraction is required with t antigen for the first stage ...	1988	2841119
transcription of the target is required for is102 mediated deletions.	we have constructed several plasmids to test the specificity of target selection for is102 associated deletions. we had previously shown that the ciii region of phage lambda is a target for is102 mediated deletions and this region was therefore introduced in psc101, where is102 resides, in such a way that it was silent, transcribed or fully expressed. the deletion selection was provided by the galactokinase system. the results we have obtained show that transcription of the target region is requ ...	1988	2841569
structural diversity of the patatin gene family in potato cv. desiree.	we have used a combined genetical and molecular approach to study the structural diversity of the patatin gene family in tetraploid solanum tuberosum l. cv. desiree (2n = 4x = 48). nine dihaploid derivatives (2n = 2x = 24) of cv. desiree were isolated by gynogenesis through prickle pollination with s. phureja juz et buk. patatin dna sequences in desiree and in the dihaploids were examined by probing southern blots of restriction endonucleases hindiii and xbai digested dna with patatin cdna regio ...	1988	2841572
purification by ammonium sulfate precipitation of bacteriophage lambda gt11 dna for restriction analysis of cloned cdna inserts.	a rapid and efficient method to purify lambda gt11 dna is described. this technique involves precipitation of intact bacteriophage particles with ammonium sulfate, followed by phage lysis with sodium dodecyl sulfate, proteinase k, and alkaline treatment. the quality of dna for subsequent restriction analysis, infectivity, subcloning, and radiolabeling is comparable to that isolated by cesium chloride banding or ion exchange chromatography. the yield of the phage dna is, however, two to eight tim ...	1988	2841887
identification of the yeast dna polymerase i gene with antibody probes.	partially overlapping fragments of the gene encoding yeast dna polymerase i have been cloned by immunological screening of a yeast genomic library constructed in the phage lambda expression vector lambda gt11. the three gene fragments we analyzed in detail encode part of a yeast protein that has been identified as yeast dna polymerase i, because it shares with this enzyme a number of antigenic determinants. in fact, the yeast protein fragments expressed by the recombinant phages react with both ...	1985	2842072
structural and functional analysis of tn4430: identification of an integrase-like protein involved in the co-integrate-resolution process.	the 4149-bp transposon tn4430 from bacillus thuringiensis is delineated by 38-bp inverted repeats and codes for a 113-kd protein that shares homology with the transposases (tnpa) of tn3, tn21 and tn501. through transpositional recombination, this protein generates the formation of co-integrates between both donor and target replicons, with duplication of tn4430 molecules. these features are characteristic of transposons of the tn3 family (class ii elements). the second step of the transposition ...	1988	2842151
activation of recf-dependent recombination in escherichia coli by bacteriophage lambda- and p22-encoded functions.	escherichia coli strains bearing wild-type and mutant alleles of various recombination genes, as well as plasmids that express recombination-related genes of bacteriophages lambda and p22, were tested for their proficiency as recipients in hfr-mediated conjugation. it was found that the homologous recombination systems of both phages could promote recombination in a recb recc mutant host. in addition, the abc function of p22, but not the gam function of lambda, was found to inhibit recombination ...	1988	2842317
restriction patterns of model dna treated with 5,6-dihydroxyindole, a potent cytotoxic intermediate of melanin synthesis: effect of u.v. irradiation.	the interaction of 5,6-dihydroxyindole, a putative cytotoxic intermediate of melanin synthesis, with model lambda phage dna has been investigated by using type ii restriction endonucleases and cscl buoyant density centrifugation. as evidenced by agarose gel electrophoresis and density gradient profiles, the 5,6-dihydroxyindole or u.v. treated dnas, restricted or not, are modified. u.v. irradiation enhances 5,6-dihydroxyindole binding to dna, but no sequence specific binding was observed. the act ...	1987	2842578
tn10 transposition promotes reca-dependent induction of a lambda prophage.	we present evidence that tn10 transposition, or a closely correlated event, induces expression of bacterial sos functions. we have found that lambda prophage induction is increased in escherichia coli lambda lysogens containing increased tn10 transposase function plus single or multiple copies of an appropriate pair of transposon ends. this increase occurs by the normal pathway for prophage induction, which involves reca-mediated cleavage of the phage lambda repressor protein. we also present ev ...	1988	2842758
helical-repeat dependence of integrative recombination of bacteriophage lambda: role of the p1 and h1 protein binding sites.	the efficiency of site-specific recombination of bacteriophage lambda was found to depend on the spacing between distant protein binding sites. insertions and deletions of up to 30 base pairs were made in the nonessential regions between the h1 and h2 protein binding sites. recombination was found to occur in substrates with changes of integral multiples of a dna helical repeat, whereas recombination was defective in substrates with nonintegral changes. the lambda recombinogenic complex is espec ...	1988	2842765
conferring operator specificity on restriction endonucleases.	mapping and manipulation of very large genomes, including the human genome, would be facilitated by the availability of a dna cleavage method with very high site specificity. therefore, a general method was devised that extends the effective recognition sequences well beyond the present 8-base pair limit by combining the specificity of the restriction endonuclease with that of another sequence-specific protein that binds tightly to dna. it was shown that the tightly binding lac or lambda repress ...	1988	2842862
the dna intermediate in yeast ty1 element transposition copurifies with virus-like particles: cell-free ty1 transposition.	yeast ty1 elements are retrotransposons that transpose via an rna intermediate found in a virus-like particle (ty-vlp). a ty-encoded reverse transcriptase activity found inside the particles is capable of giving rise to full-length reverse transcripts. the predominant form of these reverse transcripts is a full-length linear duplex dna. we have developed a cell-free system for transposition of ty1 dna molecules into a bacteriophage lambda target. purified ty-vlps and target dna are the only macr ...	1988	2843295
controllable alteration of cell genotype in bacterial cultures using an excision vector.	we have used recombinant dna techniques to construct a derivative of phage lambda, called an excision vector, which retains only those functions necessary for conditional maintenance of lysogeny and integration/excision. the tyra+ gene was cloned on this excision vector, integrated into the escherichia coli chromosome, and stably maintained and expressed under permissive conditions. upon shift to non-permissive conditions, the excision vector and its passenger gene were very efficiently excised ...	1988	2843443
[plasmids containing 2 new variants of the e.coli lacz gene].	new plasmids containing partially deleted lacz genes were obtained. these genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kd under the control of the lambda phage pr-promoter; inspite of the deletion, e. coli cells carrying new plasmids were found to possess beta-galactosidase activity. use of these plasmids as new expression vectors is suggested.	1988	2844193
cloning and sequencing of cdna encoding human dna topoisomerase ii and localization of the gene to chromosome region 17q21-22.	two overlapping cdna clones encoding human dna topoisomerase ii were identified by two independent methods. in one, a human cdna library in phage lambda was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and drosophila dna topoisomerase ii; in the other, a different human cdna library in a lambda gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to ...	1988	2845399
structure and expression of the guinea-pig alpha-lactalbumin gene.	the entire guinea-pig alpha-lactalbumin gene was isolated from a genomic dna library constructed in the bacteriophage lambda l47. the complete nucleotide sequence of the gene and its immediate 5' and 3' flanking sequences were determined and compared with those of the human and rat alpha-lactalbumin genes. this demonstrates that the size, organization and sequence of the exons is highly conserved between species, and reveals the presence of the highly conserved potential regulatory 'milk box' co ...	1988	2845947
the requirements for a high level of transposition of bacteriophage mu.	to analyse the functional and structural requirements of mu transposition a special mini-mu transposon was constructed. this mini-mu, situated on a derivative of pbr322, has an easily selectable marker gene conferring chloramphenicol resistance (cam) cloned between the ends of the mini-mu. the genes required for transposition are cloned outside the ends and are under control of the pl promoter of bacteriophage lambda. to obtain a high frequency of transposition the a and b products are required. ...	1987	2846595
the role of template superhelicity in the initiation of bacteriophage lambda dna replication.	the prepriming steps in the initiation of bacteriophage lambda dna replication depend on the action of the lambda o and p proteins and on the dnab helicase, single-stranded dna binding protein (ssb), and dnaj and dnak heat shock proteins of the e. coli host. the binding of multiple copies of the lambda o protein to the phage replication origin (ori lambda) initiates the ordered assembly of a series of nucleoprotein structures that form at ori lambda prior to dna unwinding, priming and dna synthe ...	1988	2847118
molecular cloning and expression of the imipenem-hydrolyzing beta-lactamase gene from pseudomonas maltophilia in escherichia coli.	the l-1 penicillinase structural gene, blas, from pseudomonas maltophilia was cloned into the vector pacyc184. the pmon01 recombinant plasmid selected by ampicillin resistance carried a 2.6-kilobase (kb) sau3a fragment of p. maltophilia dna and was confirmed to express l-1 beta-lactamase by comparative isoelectric focusing. a detailed physical map was constructed, and the blas structural gene was localized with a 17-mer oligonucleotide mixed probe encoding the l-1 nh2-terminal amino acid sequenc ...	1988	2847278
human dna topoisomerase i is encoded by a single-copy gene that maps to chromosome region 20q12-13.2.	cdna clones of the human top1 gene encoding dna topoisomerase i (ec 5.99.1.2) have been obtained by immunochemical screening of phage lambda libraries expressing human cdna segments, using rabbit antibodies raised against purified hela dna topoisomerase i. hybridization patterns between the cloned cdna sequences and human cellular dna and cytoplasmic mrnas indicate that human top1 is a single-copy gene. the chromosomal location of the gene has been mapped to the long arm of chromosome 20, in the ...	1988	2848244
intrachromosomal rearrangements mediated by hobo transposons in drosophila melanogaster.	the recurring intrachromosomal rearrangements observed in an unstable x chromosome, designated uc, of drosophila melanogaster are shown to be mediated by hobo transposable elements. each of 29 chromosome rearrangement breakpoints in 16 gross aberrations detected in the uc-derived x chromosomes had a hobo element. in one particular unstable x chromosome line selected for detailed studies, a hobo element was found in each of the five hot spots for rearrangements. furthermore, hobo elements at dele ...	1988	2848256
q-mediated late gene transcription of bacteriophage lambda: rna start point and rnase iii processing sites in vivo.	the location of the rna start point of in vivo q-activated late gene rna of bacteriophage lambda has been determined to be identical to the start point of in vitro 6 s rna. the 6 s rna is made early in infection and is efficiently antiterminated by q. two rnase iii cut sites are located within q-dependent rna sequences, 209 and 270 bp from the beginning of the late transcript, and lie on the stem of an inverted repeat which has features in common with previously described rnase iii processing si ...	1988	2849240
avian nephroblastomas induced by a retrovirus (mav-2) lacking oncogene. i. construction of mav-1 and mav-2 proviral restriction maps and preparation of specific proviral molecular subclones.	a 9.8 kb dna fragment containing the complete mav-1 provirus was recloned from the recombinant bacteriophage lambda 311411 (perbal et al., 1985) into the plasmid pat153. a detailed and precise restriction map of the obtained clone (pat-mav-1) was constructed. from compilation of this map and the known sequence of a variable portion of the mav-2 env gene was a restriction map of mav-2 deduced. knowledge of the detailed pat-mav-1 map facilitated the preparation of five specific proviral subclones: ...	1988	2849567
[the search for strains producing new restriction endonucleases].	the search for restrictases in 154 strains belonging to 104 species of 32 genera of microorganisms has been carried out by the method of rapid toluene assay. in 10 strains the activity of endonucleases specifically fragmenting the dna of phage lambda in the presence of mg2+ ions has been detected. restrictases pae i and pae ii formed by two pseudomonas aeruginosa strains have been identified as the true isoschizomers of restriction endonucleases sph i and sma i respectively. the results of the s ...	1988	2849847
initiation of lambda dna replication reconstituted with purified lambda and escherichia coli replication proteins.	using highly purified bacteriophage lambda and e. coli replication proteins, we were able to reconstitute an in vitro system capable of replication ori lambda-containing plasmid dna. the addition of a new e. coli factor, the grpe gene product, to this replication system reduced the level of dnak protein required for efficient dna synthesis by at least 10-fold, and also allowed the isolation of a stable dna replication intermediate. based on all available information, we propose a molecular mecha ...	1988	2850011
bacteriophage lambda cloning vectors.		1988	2850047
transposition of lambda placmu is mediated by the a protein altered at its carboxy-terminal end.	lambda placmu phages are derivatives of bacteriophage lambda that use the transposition machinery of phage mu to insert into chromosomal and cloned genes. when inserted in the proper fashion, these phages yield stable fusions to the escherichia coli lac operon in a single step. we have determined the amount of dna from the c end of phage mu present in one of these phages, lambda placmu3, and have shown that this phage carries a 3137-bp fragment of mu dna. this dna segment carries the mu c-end at ...	1988	2850974
expression in e. coli of erg: a novel gene in humans related to the ets oncogene.	we have recently cloned a novel human gene named erg related to the ets oncogene. in this report we describe the expression of the erg gene product in e. coli under the control of the tightly-regulated phage lambda pl promoter. two expression vectors were utilized for the synthesis of the cii-erg and erg-fusion proteins. all these products were recognized by peptide antibodies prepared against the predicted amino acid sequences of erg and the hu-ets-2 domain which shares homology with erg.	1987	2851121
analysis of apolipoprotein e5 gene from a patient with hyperlipoproteinemia.	we have isolated and analyzed apolipoprotein e5 gene from a patient with hyperlipoproteinemia. apolipoprotein e5 is a variant of apolipoprotein e with two additional units of positive charge and smaller apparent molecular weight than apolipoprotein e3, which is the major isoform of apolipoprotein e. the heterozygous gene of apolipoprotein e5/3 from the patient was cloned into lambda phage. the cloned apolipoprotein e genes were subcloned into a murine retrovirus shuttle vector and were expressed ...	1988	2851587
[design and synthesis of peptides capable of specific binding to dna].	in the present communication, design, synthesis and dna binding activities of the following two peptides are reported: dns-gly-ala-gln-lys-leu-ala-cly-lys-val-gly-thr-lys-val-lys-val-gl y-thr-lys-thr - val-oh (i) and [(h-ala-lys-leu-ala-thr-lys-ala-gly-val-lys-gln-gln-ser-ile-gln-leu-ile- thr- ala-aca-lys-aca)2lys-aca]2lys-val-oh (ii), where aca = nh(ch2)5co--; dns is a residue of 5-dimethylaminonaphtalene-1-sulfonic acid. peptide i contains a large fraction (ca.30%) of valyl and threonyl residu ...	1988	2851717
segments of bacteriophage lambda (orf 221) and phi 80 are homologous to genes coding for mammalian protein phosphatases.	the amino acid sequences of mammalian protein phosphatase 1 and 2a were compared pairwise with every sequence in the national biomedical research foundation protein sequence database using an exhaustive searching programme [coulson et al., comp. j. 30 (1987) 420-424]. the n-terminal half of the protein encoded by an open reading frame, orf 221, in bacteriophage lambda (nt 43,224-43,886 in the map of daniels et al. [in hendrix et al. (eds.), lambda ii. cold spring harbor laboratory, cold spring h ...	1988	2852144
troponin of asynchronous flight muscle.	troponin has been prepared from the asynchronous flight muscle of lethocerus (water bug) taking special care to prevent proteolysis. the regulatory complex contained tropomyosin and troponin components. the troponin components were tn-c (18,000 mr), tn-t (apparent mr 53,000) and a heavy component, tn-h (apparent mr 80,000). the troponin was tightly bound to tropomyosin and could not be dissociated from it in non-denaturing conditions. a complex of tn-t, tn-h and tropomyosin inhibited actomyosin ...	1988	2852258
or3 operator of bacteriophage lambda in a 23 base-pair dna fragment: sequence-specific 1h nmr assignments for the non-labile protons and comparison with the isolated 17 base-pair operator.	sequence-specific 1h nmr assignments are presented for a non-selfcomplementary 23-base-pair dna duplex of molecular weight 15,000 daltons, containing the or3 repressor binding site of bacteriophage lambda as the central core. the nmr techniques used were mainly phase-sensitive two-dimensional noe and 2q spectroscopy, the latter to overcome overlap problems within the spectral region of the deoxyribose spin-systems. direct sequential noe connectivities are observed between adenine 2 h and deoxyri ...	1988	2853668
jekyll, a family of phage-plasmid shuttle vectors.	a series of shuttle vectors has been constructed, which consist of a plasmid carrying a polylinker sequence and an m13 origin integrated into a lambda vector. a short direct repeat flanking the plasmid allows plasmid excision by homologous recombination. sequences are cloned into unique restriction sites within the plasmid, and can be recovered either in phage or plasmid form, or can be packaged further as single-stranded dna phage. these vectors therefore combine the efficiency of phage lambda ...	1988	2854093
isolation of mutants in a dna methyltransferase through mcrb-mediated restriction.	a procedure has been developed that permits the positive selection of mutants in a dna methyltransferase (mtase) gene. the stringency of this selection can be varied so as to yield null mutants only, or a mixture of null and partially defective mutants. the procedure was developed with the pvuii mtase gene (pvuiim), which was subcloned into a bacteriophage lambda vector. growth of this lambda pvuiim construct on an mcrb+ host selected for non-methylating mutants, and the stringency of selection ...	1988	2854810
[rapid isolation of phage lambda dna].	a modified procedure in two versions (micro, for 10 ml of phage lysate, and macro, for 200-500 ml) is described for preparing lambda phage dna. the main advantage of the modified method is that it gives a possibility to isolate high-quality dna from lambda phage lysates in 2-3 hrs. only standard solutions (te, nacl, sds, mgcl2, edta, rnase a) were used throughout the whole protocol. incubation with dnase i and proteinase k was omitted and in microvariant concentration of the phage by peg 6000 wa ...	1988	2855252
expression of the sos genes of escherichia coli in salmonella typhimurium.	to lysogenize salmonella typhimurium by lambda phage, a region of 10.2 kb of escherichia coli dna carrying the nusa gene was cloned in a s. typhimurium strain containing a f'112 plasmid which codifies for the lamb region of e. coli. the strain of s. typhimurium obtained in this way, was lysogenized by lambda c indo- bacteriophage harboring either a fusion between reca1 or sfia genes of e. coli with lacz gene. likewise, pse143 plasmid with a umu c::lacz fusion was introduced in s. typhimurium. af ...	1985	2855830
cloning and expression in escherichia coli of histidine utilization genes from pseudomonas putida.	a library of the pseudomonas putida chromosome, prepared through the use of the cosmid pjb8 ligated to a partial sau3a digest of bacterial dna, followed by in vitro packaging into bacteriophage lambda particles, was used to construct a strain of escherichia coli which contained the genes for histidine utilization. this isolate produced a repressor product and all five enzymes required in pseudomonas spp. for histidine dissimilation, whereas none of these could be detected in the nontransduced pa ...	1985	2858467
genetic analysis of the phase variation control of expression of type 1 fimbriae in escherichia coli.	expression of type 1 fimbriae in escherichia coli exhibits phase variation, whereby individual cells can alternate between states of organelle expression (fim+) and nonexpression (fim-). strains with a fimd-lac operon fusion, in which lac, rather than fimd, expression is under the control of the fimd promoter, undergo lac+ in equilibrium lac- phase variation, instead. after positioning a lambda prophage adjacent to the operon fusion, we were able to isolate specialized lambda phage carrying both ...	1985	2859269
characterization of the gene encoding glutamine synthetase i (glna) from bradyrhizobium japonicum.	we have isolated the bradyrhizobium japonicum gene encoding glutamine synthetase i (glna) from a phage lambda library by using a fragment of the escherichia coli glna gene as a hybridization probe. the rhizobial glna gene has homology to the e. coli glna gene throughout the entire length of the gene and can complement an e. coli glna mutant when borne on an expression plasmid in the proper orientation to be transcribed from the e. coli lac promoter. high levels of glutamine synthetase activity c ...	1985	2859270
production of oncogene-specific proteins and human t-cell leukemia (lymphotropic) retrovirus (htlv-i) envelope protein in bacteria and its potential for use in human cancers and seroepidemiological surveys.	the oncogenes coding for the harvey murine sarcoma virus p21ras protein as well as those coding for myc, myb, and mht products were fused to the amino-terminal portion of the bacteriophage lambda cii gene on the expression vector pjl6. in addition two regions of the gene for the human t-cell leukemia virus subgroup i (htlv-i) envelope were expressed in our bacterial system. each of 11 human sera tested that had been shown to contain antibodies to htlv-i or -ii by an enzyme-linked immunosorbent a ...	1985	2861893
translational initiation frequency of atp genes from escherichia coli: identification of an intercistronic sequence that enhances translation.	the c, b and delta subunit genes of the escherichia coli atp operon were cloned individually in an expression vector between the tac fusion promoter and the galk gene. the relative rates of subunit synthesis directed by the cloned genes were similar in vitro and in vivo and compared favourably with the subunit stoichiometry of the assembled proton-translocating atp synthase of e. coli in vivo. the rate of synthesis of subunit c was at least six times that of subunit b and 18 times that of subuni ...	1985	2862030
regulation of bacillus subtilis glutamate synthase genes by the nitrogen source.	the wild-type alleles of the glta292 and gltb1 mutations of bacillus subtilis have been identified in banks of b. subtilis dna cloned in phage lambda. these mutations are thought to define the genes for the two subunits of glutamate synthase. sequences having transforming activity for each allele were subcloned in plasmids and used as hybridization probes for measurements of the rates of synthesis and steady-state levels of glt mrnas under different growth conditions. for both glta and gltb, the ...	1985	2863256
proton conduction by subunit a of the membrane-bound atp synthase of escherichia coli revealed after induced overproduction.	transcriptional fusions between the phage lambda promotor pr and atp synthase genes, atp, on plasmid pbr322 were constructed in order to study the effects upon growth and physiology of escherichia coli of induced overproduction of h+-atpase subunits. constitutive overproduction of the complete enzyme had earlier been found to result in decreased growth rate and cytological defects. when a 15-fold overproduction of subunit a alone, or together with subunit c, or with all other atp synthase subuni ...	1985	2866956
the role of adenylyltransferase and uridylyltransferase in the regulation of glutamine synthetase in escherichia coli.	the regulation of gs activity involves two nucleotidylation cycles, the uridylylation cycle of pii and the adenylylation cycle of gs, which are catalyzed by two converter enzymes, uridylyltransferase and adenylyltransferase, respectively. the converter enzymes sense the fluctuation in the availability of nitrogen and accordingly regulate the activity of gs. on the other hand, the posttranslational modification of gs is tightly coupled to the transcriptional regulation of the glna gene by unmodif ...	1985	2868842
cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme a synthase from the hamster. ii. isolation of the gene and characterization of the 5' flanking region.	cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) synthase and microsomal hmg-coa reductase are sequential enzymes in the cholesterol biosynthetic pathway; both are negatively regulated by cholesterol. in this paper, we report the isolation of overlapping bacteriophage lambda clones that encompass the gene for hamster cytoplasmic hmg-coa synthase. the gene spans 20 kilobases and contains 11 exons and 10 introns. under conditions of high-level expression in cultured hamster cells and ha ...	1986	2869036
bordetella pertussis filamentous hemagglutinin gene: molecular cloning of a potential coding sequence.	we have constructed an expression library of bordetella pertussis dna sequences, cloned in escherichia coli. random dna fragments were inserted into the beta-galactosidase gene of bacteriophage lambda gt11 and plaques were screened with antibodies directed against filamentous hemagglutinin. the antigen-positive clone obtained expressed b. pertussis sequences as polypeptides fused to beta-galactosidase. the fusion polypeptides reacted both with antibodies to beta-galactosidase and with antibodies ...	1985	2872112
synthesis of maize chloroplast atp-synthase beta-subunit fusion proteins in escherichia coli and binding to the inner membrane.	the maize chloroplast gene for the beta subunit (atpb) of the chloroplast cf1 component of atpase from maize, when fused to either the lacz or ral genes in the vectors pmc1403 or phub4, is expressed in escherichia coli as a fusion protein with beta-galactosidase or with bacteriophage lambda ral sequences. some of the fusion proteins are converted to a membrane-bound form, as determined by differential and sucrose-gradient centrifugation. the specificity of membrane binding has been examined usin ...	1986	2872138
structure of rat dna polymerase beta revealed by partial amino acid sequencing and cdna cloning.	a cdna library of newborn rat brain poly(a)+ rna in phage lambda gt11 was screened with a polyclonal antibody against chicken dna polymerase beta. one positive phage was isolated and purified after testing 2 x 10(7) recombinants. this phage, designated lambda pol beta-10, contained an 1197-base-pair cdna insert that corresponded to a mrna with a poly(a) sequence at the 3' terminus and a single, long open-reading frame of 957 bases. the open-reading frame, starting 44 residues from the 5' end of ...	1986	2873575
phototoxic potential of mequitazine, a phenothiazine derivative, as determined by the photosensitized action on microbiological systems.	in order to determine phototoxic potential of mequitazine (mqz), a phenothiazine derivative, in vitro tests were attempted using microbiological systems. when escherichia coli was irradiated with ultraviolet-a light in the presence of mqz, the surviving fraction was decreased with increasing fluence. irradiation of bacteriophage lambda in the presence of the drug decreased the surviving fraction as well. possible targets for the photosensitized action in these systems were discussed.	1986	2876782
a straightforward approach to isolate dna sequences with potential linkage to the retinoblastoma locus.	from a human-chinese hamster somatic cell hybrid a clone was derived containing chromosome 13 in duplicate as its only human material. this clone was used to construct a human chromosome 13-specific recombinant dna-library. overlapping sau3ai dna sequences (11.9-17.2 kb) from the cell hybrid were inserted into the lambda phage vector embl4. from eleven recombinants having a human insert thirteen putative unique dna sequences were isolated and cloned into the plasmid vector pbr329. a human-mouse ...	1986	2877932
secondary attachment site for bacteriophage lambda in the guab gene of escherichia coli.	lambda gua transducing bacteriophages were used to identify and sequence the secondary attachment site for lambda in the guab gene of escherichia coli. the sequence matched the primary core sequence at nine positions, and a putative integrase binding-site overlapped the left core-arm junction. recombinational crossover occurred between nucleotides -3 and +2 of the core region.	1986	2877966
morphogenetic expression of bacteroides nodosus fimbriae in pseudomonas aeruginosa.	type 4 fimbriae are found in a range of pathogenic bacteria, including bacteroides nodosus, moraxella bovis, neisseria gonorrhoeae, and pseudomonas aeruginosa. the structural subunits of these fimbriae all contain a highly conserved hydrophobic amino-terminal sequence preceding a variable hydrophilic carboxy-terminal region. we show here that recombinant p. aeruginosa cells containing the b. nodosus fimbrial subunit gene under the control of a strong promoter (pl, from bacteriophage lambda) prod ...	1987	2878919
a plasmid vector for cloning and expression of gene segments: expression of an htlv-i envelope gene segment.	we describe a cloning-expression vector system for selecting dna fragments containing open reading frames (orfs) and expressing them as beta-galactosidase (beta gal) hybrid fusion proteins. the plasmid vector, pws50, utilizes the very strong and easily regulated bacteriophage lambda promoter pl, and the efficient translation initiation signals of the n-terminal segment of the lambda cii gene. fused distally to and out of translational phase with cii is the e. coli lacz gene, lacking its own tran ...	1986	2881844
identification and cell type specificity of the tyrosine hydroxylase gene promoter.	genomic dna encoding the rat tyrosine hydroxylase (th) gene was isolated from a lambda phage library using a nick-translated fragment from a cdna clone for rat th. we have determined the initiation site for th rna synthesis and have sequenced 1100 bases of the primary transcript and 5' flanking region. the 5' end of the transcript is the same in several rat tissues in which th is expressed as well as in rat pheochromocytoma cells (pc). rna prepared from pc cells that had been stimulated with dex ...	1987	2882469
identification of 28 dna fragments that detect rflps in 13 distinct physical regions of the short arm of chromosome 5.	a series of 175 lambda phage carrying human inserts isolated from a library that is specific for the short arm of human chromosome 5 (5p) have been regionally mapped on 5p using a deletion mapping panel of 16 human-hamster cell hybrids, each of which contains a chromosome 5 with a different deletion in the short arm. seventy-five single copy dna fragments were screened with 12 restriction enzymes for their ability to detect restriction fragment length polymorphisms (rflps). twenty-eight of these ...	1987	2884625
dna sequence and regional assignment of the human follicle-stimulating hormone beta-subunit gene to the short arm of human chromosome 11.	a human genomic dna fragment in phage lambda containing fshb, the gene for the beta-subunit of human follicle-stimulating hormone (fsh-beta), was analyzed and the nucleotide sequence of the region of the clone encoding fsh-beta was determined. a subclone of the lambda phage containing 67% of fsh-beta coding sequence was used as hybridization probe to determine the human chromosomal location of fshb. a panel of mouse-human somatic cell hybrids containing reduced numbers of human chromosomes was s ...	1987	2885163
a human papilloma virus type 11 transcript encoding an e1--e4 protein.	the human papilloma virus (hpv) associated with a genital wart (condyloma acuminatum) was determined to be type 11. the majority of the viral dna molecules were monomeric circles present in the cells at high copy number, as demonstrated by one- and two-dimensional agarose gell electrophoretic separation followed by southern blot analysis. a cdna library in phage lambda gt11 was constructed from poly(a)-selected mrna recovered from the tissue. recombinant clones corresponding to the most abundant ...	1987	2887066
replacement of potassium chloride by potassium glutamate dramatically enhances protein-dna interactions in vitro.	although protein-nucleic acid interactions exhibit dramatic dependences on both ion concentration and type in vitro, large variations in intracellular ion concentrations can occur in escherichia coli and other organisms without apparent effects on gene expression in vivo. e. coli accumulates k+ and glutamate as cytoplasmic osmolytes. the cytoplasmic k+ concentration in e. coli varies from less than 0.2 to greater than 0.9 m as a function of external osmolarity; corresponding cytoplasmic glutamat ...	1987	2887198
a rapid and efficient screening method for dna restriction fragment length polymorphisms.	genomic loci displaying dna sequence polymorphisms represent useful landmarks on the genetic linkage map. we describe an integrated experimental approach to facilitate the detection of dna restriction fragment length polymorphisms (rflp) with useful allelic frequencies at loci covered by cloned dna sequences. the essential feature of the screening method presented is the pooling of dna from unrelated individuals for southern blot hybridization analyses using non-repetitive dna sequences identifi ...	1987	2891458
suppression of the escherichia coli ssb-1 mutation by an allele of groel.	a series of spontaneous suppressors to the temperature-sensitive phenotype of the single-stranded dna-binding protein mutation ssb-1 were isolated. a genomic library of ecori fragments from one of these suppressor strains was prepared by using pbr325 as the cloning vector. a 10.0-kilobase class of inserts was identified as carrying the ssb-1 gene itself. a second class of 8.3-kilobase inserts was shown to contain the groe region by (i) restriction analysis, (ii) southern hybridization of the 8.3 ...	1988	2897690
control of cell division by sex factor f in escherichia coli. iii. participation of the groes (mopb) gene of the host bacteria.	cell division of f+ bacteria is coupled to dna replication of the f plasmid. two plasmid coded genes, leta (ccda) and letd (ccdb) are indispensable for this coupling. to investigate bacterial genes that participate in this coupling, we attempted to identify the target of the division inhibitor (the letd gene product) of the f plasmid. two temperature-sensitive growth defective mutants were screened from bacterial mutants that escaped the letd product growth inhibition that occurs in hosts carryi ...	1988	2901493
cloning and expression in escherichia coli of the perfringolysin o (theta-toxin) gene from clostridium perfringens and characterization of the gene product.	the gene encoding perfringolysin o, the thiol-activated hemolysin from clostridium perfringens (atcc 13124), was cloned and expressed in escherichia coli. a gene library of c. perfringens chromosomal dna was constructed in bacteriophage lambda embl3. a recombinant was identified that produced a hemolysin that was inhibited by cholesterol and was tentatively identified as perfringolysin o. subcloning experiments localized the perfringolysin o gene (pfo) to a 1.8-kilobase region on the cloned chro ...	1988	2903127
cloning and nucleotide sequence of human gamma-glutamyl transpeptidase.	we have identified the gene for human gamma-glutamyl transpeptidase [ggt; glutamine:d-glutamyl-peptide 5-glutamyltransferase (also called gamma-glutamyltransferase), ec 2.3.2.2] in a bcr gene-related region located in band q11----qter of chromosome 22. two cdnas complementary to the ggt mrna have been isolated from a human placental library constructed in phage lambda gt11. the largest cdna has a size of 2535 base pairs (bp) and an open reading frame of 1707 nucleotides encoding 569 amino acids. ...	1988	2904146
partial digestion with restriction enzymes of ultraviolet-irradiated human genomic dna: a method for identifying restriction site polymorphisms.	a method for partial digestion of total human dna with restriction enzymes has been developed on the basis of a principle already utilized by p.a. whittaker and e. southern (1986, gene 41: 129-134) for the analysis of phage lambda recombinants. total human dna irradiated with uv light of 254 nm is partially digested by restriction enzymes that recognize sequences containing adjacent thymidines because of tt dimer formation. the products resulting from partial digestion of specific genomic region ...	1988	2906329
mouse thymidine kinase: the promoter sequence and the gene and pseudogene structures in normal cells and in thymidine kinase deficient mutants.	the mouse genome carries one gene and two pseudogenes for cytoplasmic thymidine kinase. the overall structure of these genes was determined with the help of cosmids and lambda phage clones and the upstream sequence containing the promoter was determined. the data allow an allocation of bands seen in the complex patterns of genomic southern blots obtained from the dna of wild type cells and of thymidine kinase deficient mutants to the gene as well as to the two pseudogenes. the much used ltk cell ...	1989	2911464
cdna encoding an immunogenic region of a 22 kilodalton surface protein of eimeria acervulina sporozoites.	cdna encoding an immunogenic region of a 22 kda surface protein of eimeria acervulina sporozoites was cloned and expressed in the bacteriophage lambda gt11 vector. the recombinant beta-galactosidase fusion protein, designated ma1, has an apparent molecular size of 125 kda. immunofluorescence staining of intact e. acervulina sporozoites and merozoites and immunoblotting of 125i-surface labeled protein from both stages revealed exclusive expression of the cloned cdna in the sporozoite stage. the g ...	1989	2927444
anti-liver-kidney microsome antibody type 1 recognizes human cytochrome p450 db1.	anti-liver-kidney microsome antibody type 1 (lkm1), present in the sera of a group of children with autoimmune hepatitis, was recently shown to recognize a 50 kda protein identified as rat liver cytochromes p450 db1 and db2. high homology between these two members of the rat p450 iid subfamily and human p450 db1 suggested that anti-lkm1 antibody is directed against this human protein. to test this hypothesis, a human liver cdna expression library in phage lambda gt-11 was screened using rat p450 ...	1989	2930529
[w-mutagenesis in the bisulfite-treated lambda phage].	survival of phage lambda ci857 inactivated by bisulfite (ph 5.6, 37 degrees c) is higher (the dose modification factor approx. 1.2) and frequency of bisulfite-induced c-mutations 2-4-fold lower on the lawn of the wild-type strain ung+, as compared to ung-1 mutant deficient in uracil-dna glycosylase. irradiation of host cells by a moderate uv dose inducing sos repair system enhances the frequency of bisulfite-induced c-mutations 2-3-fold in the wild-type (ung+) host, but not in the ung-1 mutant. ...	1985	2931321
a cii-responsive promoter within the q gene of bacteriophage lambda.	a site within the phage lambda q gene shares homology with the cii-activated promoters, pe and pi, and is oriented in the direction opposite to that of q gene transcription. a dna fragment containing this site can serve as a template for cii-activated transcription in vitro. to ask if this presumptive cii control site functions as a cii-activated promoter in vivo, a restriction fragment containing this promoter has been cloned on a plasmid so that synthesis of beta-galactosidase will be under it ...	1985	2931322
differential expression of influenza n protein and neuraminidase antigenic determinants in escherichia coli.	two influenza gene products of similar size and codon usage have been expressed in escherichia coli under control of the phage lambda pr promoter. the influenza n protein (np) was expressed in its entirety after fusion to a short (12 amino acid) segment of the lambda cro gene product and constituted about 1-2% of total soluble cell protein after induction. by contrast, constructions using the full length neuraminidase (na) gene failed to give rise to detectable amounts of na antigen after fusion ...	1985	2931323
characterization of a third, cii-dependent, coordinately activated promoter on phage lambda involved in lysogenic development.	lysogenic development by bacteriophage lambda is known to require the coordinate expression of two phage operons. coordinate control is achieved by a positive regulatory mechanism which activates transcription from the promoters of these operons, pre and pi. the positive effector is the phage regulatory protein cii. we now identify and characterize a third cii-dependent transcription unit on phage lambda, which is positioned in the middle of the q regulatory gene and has an anti-q orientation. w ...	1985	2931430
pyrimidine dimers are not the principal pre-mutagenic lesions induced in lambda phage dna by ultraviolet light.	experiments were performed to examine the role of cyclobutyl pyrimidine dimers in the process of mutagenesis by ultraviolet (u.v.) light. lambda phage dna was irradiated with u.v. and then incubated with an escherichia coli photoreactivating enzyme, which monomerizes cyclobutyl pyrimidine dimers upon exposure to visible light. the photoreactivated dna was packaged into lambda phage particles, which were used to infect e. coli uvr- host cells that had been induced for sos functions by ultraviolet ...	1985	2931533
[swine serum increases hybridoma stability].	three hybridoma cell lines producing monoclonal antibodies to lambda phage were cultured for 9 months in dulbecco's modified eagl's medium supplemented with 10% fetal calf serum or with 5% swine serum. under these conditions all the clones had the same proliferation rates and maximum population densities. the cultuvation in the medium with swine serum enhanced the stability of hybridomas: all clones retained the ability to produce antibodies throughout the experiment; one of the clones examined ...	1985	2931872