Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| karyotypic analysis of two related cervical carcinoma cell lines that contain human papillomavirus type 18 dna and express divergent differentiation. | the cell lines c-4i and c-4ii were established in culture from a nonkeratinizing squamous carcinoma of the uterine cervix. both lines contain human papillomavirus (hpv) type 18 dna (brandt et al., cold spring harbor laboratories, 5:179, 1987) and both are hypodiploid with similar, but not identical, karyotypes. each line expresses multiple characteristics of ectocervical epithelial differentiation, but the characteristics differ between the lines. in the present study, g banding of the lines sho ... | 1989 | 2540900 |
| an atypical case of penile carcinoma in situ associated with human papillomavirus dna type 18. | we report an unusual case of penile carcinoma in situ in a heterosexual man. the histologic study showed a severe dysplasia, and the typing of human papillomavirus dna, performed at the pasteur institute, disclosed a type 18, which is usually associated with penile and cervical carcinomas. the identification of an oncogenic human papillomavirus type in the penile lesions, such as type 18 found in this case, should lead to a search for cervical carcinoma in the sexual partner. | 1989 | 2541182 |
| pills, partners and preventive prospects: in-situ cancer of the cervix. | australian women face a lifetime risk of dying of cervical cancer of 1 in 250. current epidemiological evidence suggests that the age at 1st sexual intercourse and the number of sexual partners are important risk factors for cervical carcinogenesis. other risk factors for invasive cervical cancer include cigarette smoking, deficiencies of dietary and plasma micronutrients, and oral contraceptive (oc) use. the recently documented increased risk of invasive cervical cancer associated with longt ... | 1989 | 2541323 |
| colposcopy of condylomatous men. | the male partners of women diagnosed with human papilloma virus (hpv) or dysplasia were examined colposcopically. anogenital infection was found in 82% of the men, and dysplasia in two of the partners of dysplastic women. men may not be merely asymptomatic carriers of hpv. | 1989 | 2541328 |
| human papillomavirus infection and cervical cancer in latin america. | to evaluate a possible association between infection with human papillomavirus (hpv) and cervical cancer, we performed a multicenter case-control study in latin america of 759 cases of invasive cervical cancer and 1467 randomly selected age-matched controls. demographic, sexual, behavioral, and other clinical data were obtained by interview, and hpv dna was assayed in cervical-swab specimens with use of filter in situ hybridization. cervical infection with hpv 16 or 18 or both was strongly assoc ... | 1989 | 2541336 |
| dna of human papillomavirus type 16 in dysplastic and malignant lesions of the conjunctiva and cornea. | human papillomaviruses are receiving attention for their role in the pathogenesis of cancer, especially cancer of the anogenital tract. although strains of human papillomavirus are associated with benign lesions of the conjunctiva, their association with conjunctival dysplastic lesions and carcinomas has remained unclear. we examined a group of neoplastic lesions of the conjunctiva for the presence of dna sequences for human papillomavirus types 16 and 18, using in vitro gene amplification with ... | 1989 | 2541337 |
| induction of human cervical squamous cell carcinoma by sequential transfection with human papillomavirus 16 dna and viral harvey ras. | clinical and epidemiological data are consistent with the hypothesis that human papillomaviruses (hpvs) are a factor in genital, particularly cervical cancer. although hpv16 and 18 are found primarily in cervical malignancy, the transfection of hpv16 or 18 dna into cervical cells results in immortalization but not tumorigenicity. the addition of activated ha-ras, an oncogene found in some cervical cancers expressing hpv16 or 18, to hpv16-immortalized human cervical cells results in malignancy as ... | 1989 | 2541388 |
| successive steps in the process of immortalization identified by transfer of separate bovine papillomavirus genes into rat fibroblasts. | transfer of neor and bovine papillomavirus type 1 (bpv1) dna into rat embryo fibroblasts led to colony formation in g418-containing medium, with no detectable background in controls with neor dna alone. more than 50% of the drug-resistant clones could be further propagated in culture. the genetic functions of bpv1 involved in colony formation and in long-term immortalization were investigated by both translation termination mutations in the full-length genome, which inactivate individual open re ... | 1989 | 2541438 |
| identification of dna-protein interactions and enhancer activity at the 5' end of the upstream regulatory region in human papillomavirus type 11. | we have examined the 5' end of the noncoding region of the genome of a human papillomavirus, hpv-11, for regulatory elements using permissive host cells. this region of unknown function in the upstream regulatory region (urr) is known to have unusual dna structure and frequently contains rearrangements which are associated with some more virulent isolates. this 5' 269-bp fragment was found to exhibit both specific dna-protein binding using laryngeal papilloma protein extracts and enhancer activi ... | 1989 | 2541536 |
| human papillomavirus type 16 e7 protein expressed in escherichia coli and monkey cos-1 cells: immunofluorescence detection of the nuclear e7 protein. | human papillomavirus type 16 e7 protein, expressed in escherichia coli as fusion protein lac alpha peptide-e7 (lac-e7), was purified by electrophoresis and used to immunize rabbits to raise antisera. the anti-lac-e7 sera recognized another bacterially expressed fusion protein trpe-e7 by the western blot method. the antisera were used to identify e7 protein transiently expressed in monkey cos-1 cells from an sv40-derived expression plasmid containing e7 gene. like the e7 protein from caski cells, ... | 1989 | 2541550 |
| macrophage killing of human papillomavirus type 16-transformed cells. | papillomaviruses (hpv) are involved in proliferation of epithelial cells and are implicated in several human malignancies. we determined the capability of activated macrophages to exert cytostatic and/or cytolytic activities against hpv type 16 dna-transformed cells. both nih-3t3 and a31-3t3 cells transformed with hpv dna were susceptible to killing by activated macrophages; however, transformed and nontransformed cells were not susceptible to killing by soluble mediators. the hpv-transformed ce ... | 1989 | 2541555 |
| detection of the human papillomavirus 6b e2 gene product in genital condyloma and laryngeal papilloma tissues. | we have previously reported that the e2 open reading frame of human papillomavirus type 6b (hpv6b) was expressed in bombyx mori cell line bm-n as a fusion protein with baculovirus polyhedrin. here we show that antibody against this e2-polyhedrin fusion protein recognized a putative gene product as a 49-kda protein in some genital condyloma and laryngeal papilloma tissues, which were associated with hpv6 and hpv11, by immunoblotting analysis. the antibody reacted also with antigens in the nucleus ... | 1989 | 2541557 |
| juvenile and adult laryngeal papillomata: classification by in-situ hybridization for human papillomavirus. | we report the application of an in-situ hybridization technique for the demonstration of human papillomavirus (hpv) employing a biotin-streptavidin-polyalkaline phosphatase complex to paraffin processed tissue from a series of patients with laryngeal papillomata. all cases of juvenile papillomata, whether solitary or multiple, proved positive for hpv types 6 and/or type 11. however, only two cases of adult solitary papillomata and five cases of adult multiple papillomata were positive for hpv ty ... | 1989 | 2541952 |
| detection of human papillomavirus dna in fine-needle aspirations of metastatic squamous-cell carcinoma of the uterine cervix using the polymerase chain reaction. | routinely processed fine-needle aspirations of metastatic squamous-cell carcinoma (scc) were analyzed for human papillomavirus type 16 (hpv-16) using the polymerase chain reaction (pcr), an in vitro dna amplification method. hpv-16 dna was detected in five of seven (71%) metastases from scc of the uterine cervix. in two cases in which the primary tumor was available for comparison, the hpv-16 dna content of the primary tumor and of the metastasis was identical. hpv-16 was not found in a metastat ... | 1989 | 2541988 |
| continued expression of hpv-16 e7 protein is required for maintenance of the transformed phenotype of cells co-transformed by hpv-16 plus ej-ras. | the close association between hpv-16 and cervical cancer implies some role for the virus in development of this cancer. recent studies have shown that the hpv-16 e7 gene encodes the major transforming activity of the virus in baby rat kidney (brk) cell transformation assays. to investigate the requirement for continued e7 expression in brk cells transformed by hpv-16 e7 plus ej-ras, we have developed a system for inducible expression of the e7 gene. the studies reported here show that continued ... | 1989 | 2542020 |
| reproductive and sexual risk factors and human papilloma virus infection in cervical cancer among hong kong chinese. | in this case-control study of cervical neoplasia in hong kong, 48 dysplasia and 68 cancer patients were matched with 116 surgical in-patient controls. the cases had an earlier age at first intercourse and more sex partners than the controls. the cases' husbands also had more sex partners and were less likely to use condoms regularly. in a multiple regression model the odds ratio were 6.0 (95% confidence interval 2.5-14.6) for the husband having more than one sex partner, 4.0 (1.5-10.7) for the w ... | 1989 | 2542172 |
| two colour dna in situ hybridization for the detection of two viral genomes using non-radioactive probes. | methods for the simultaneous detection of two virus types in cytological preparations or tissue sections by non-radioactive in situ hybridization were investigated. as a model system, caski cells, which have human papilloma virus type 16 (hpv16) dna integrated in their cellular genome, were in vitro infected with herpes simplex virus 2 (hsv2). dna probes for both viruses were labeled with biotin, acetylaminofluorene (aaf), and transaminated-sulfonated cytosine (ts-modified). best results were ob ... | 1989 | 2542189 |
| the human papillomavirus: an epidemic. | 1989 | 2542214 | |
| human papillomavirus coinfections of the vulva and uterine cervix. | dna filter in situ hybridisation (fish) was used to determine the presence of human papillomavirus (hpv) genotypes 6/11, 16/18, and 31/33 in cell scrapes of the cervix and vulva of 128 women who had precancerous lesions and/or hpv infection of the cervix diagnosed by cytology, colposcopy, and histology. hpv-dna was found in 87 (68%) vulval and 95 (74%) cervical cell scrapes, and in both the vulval and cervical scrapes of 73 (57%) women, but not in either the vulva or the cervix of 19 women (15%) ... | 1989 | 2542434 |
| detection and typing of human papillomavirus infection of the uterine cervix by dot blot hybridisation: comparison of scrapes and biopsies. | paired scrapes and biopsies from 100 women attending a routine colposcopy clinic were examined by dot blot dna hybridisation for infection with human papillomavirus (hpv) types 6/11, 16, 18, and 31; 51% of the scrapes and 50% of the biopsies were positive for hpv infection. scrapes detected more hpv 18 (10% vs. 2%, p = less than 0.05) and hpv 31 (7% vs. 3%, not significant) than did the biopsies, but biopsies detected more hpv 16 (42% vs. 33%, not significant). comparison of the results for each ... | 1989 | 2542438 |
| increased detection rate of human papillomavirus in cervical scrapes by the polymerase chain reaction as compared to modified fish and southern-blot analysis. | cervical scrapes from 80 women with a positive cytology result were tested for the presence of human papillomavirus (hpv) using the polymerase chain reaction (pcr) and compared to the results obtained with the modified filter in situ hybridisation (fish) and the southern-blot techniques. the sensitivity of the modified fish and the southern-blot was similar, and hpv was detected in 46% of the patients. the sensitivity of the pcr appeared to be higher, and hpv was detected in 70% of the patients. ... | 1989 | 2542439 |
| high prevalence of human papillomavirus infection and possible association with betel quid chewing and smoking in oral epidermoid carcinomas in taiwan. | seventeen oral epidermoid carcinomas, three oral papillomas, and 17 normal gingival tissues were tested for the presence of human papillomavirus (hpv) types 6, 11, 16, and 18 sequences by southern blot hybridization. episomal hpv-16 sequences in various amounts were detected in 76.4% of the oral carcinomas and in all three cases of papilloma. however, only one of the 17 normal tissues was hpv positive with an unknown type. none of the samples contained hpv-6, -11, or -18 sequences. examination o ... | 1989 | 2542446 |
| a point mutational analysis of human papillomavirus type 16 e7 protein. | the e7 open reading frame of human papillomavirus type 16 (hpv16) has been shown to be selectively retained in cervical tumors and to encode both transforming and trans-activating functions in murine cells, supporting the notion that expression of e7 contributes towards the progression of premalignant cervical lesions. a comparison among e7 sequences of different hpv types reveals some homology at the amino acid level. of particular interest are two regions, one which contains significant homolo ... | 1989 | 2542578 |
| cloning and partial dna sequencing of two new human papillomavirus types associated with condylomas and low-grade cervical neoplasia. | using low-stringency southern blot analysis and cloning in lambda bacteriophage, two new human papillomavirus types (hpv-43 and hpv-44) were identified and their dnas were cloned from vulvar tissues. the isolates were characterized by restriction endonuclease mapping and shown to be new hpv types on the basis of their minimal hybridization with all other known hpv types at high stringency. both hpvs are most closely related to types 6, 11, and 13. hpv-43 did not exhibit any cross-reactivity with ... | 1989 | 2542593 |
| identification of a novel constitutive enhancer element and an associated binding protein: implications for human papillomavirus type 11 enhancer regulation. | the human papillomavirus type 11 enhancer, when linked to the minimal simian virus 40 early promoter, has been dissected into two domains in monkey kidney cv-1 cells, one being constitutive (designated cei) and the other inducible by trans-acting e2 proteins encoded by homologous and heterologous papillomaviruses (h. hirochika, t.r. broker, and l.t. chow, j. virol. 61:2599-2606, 1987; h. hirochika, r. hirochika, t.r. broker, and l.t. chow, genes dev. 2:54-67, 1988). we have demonstrated that the ... | 1989 | 2542607 |
| male partners of women with genital human papillomavirus infection. an assessment of colposcopic abnormalities by histological examination and human papillomavirus hybridization. | men whose female sexual partners showed histological evidence of human papillomavirus infection were examined. human papillomavirus dna was identified in 29 of 35 biopsy samples of colposcopically-identified penile lesions. human papillomavirus strains that were related to human papillomavirus genotypes 6/11 were observed most commonly (seven of eight patients) in the partners of patients with warty atypia or condylomata, while human papillomavirus strains that were related to human papillomavir ... | 1989 | 2542738 |
| human papillomavirus dna in glandular dysplasia and microglandular hyperplasia: presumed precursors of adenocarcinoma of the uterine cervix. | presumed precursors of adenocarcinoma of the uterine cervix were investigated with specific techniques to identify human papillomavirus (hpv) dna. the presence of hpv dna in 36 lesions of glandular dysplasia and 16 lesions of microglandular hyperplasia of the uterine cervix was studied by in situ hybridization using 3h-labeled hpv 16 and hpv 18 dna probes. only two of 36 lesions (6%) of glandular dysplasia contained hpv 18 dna, although 64% of coexisting adenocarcinoma in situ, microinvasive ade ... | 1989 | 2542853 |
| hairy leukoplakia: an aids-associated opportunistic infection. | hairy leukoplakia has been defined and confirmed as a specific oral mucosal marker for human immunodeficiency virus infection. light microscopic findings include alteration of spinous layer cells, which contain nuclear chromatin abnormalities. enlargement of spinous cells with ballooning degeneration is also characteristic. ultrastructural morphology has defined the presence of large numbers of herpes-type virus particles within the intranuclear, cytoplasmic, and intercellular regions. morpholog ... | 1989 | 2542858 |
| oral lesions mimicking hairy leukoplakia: a diagnostic dilemma. | oral hairy leukoplakia is seen in immunosuppressed persons infected with the human immunodeficiency virus and is a predictor of the development of acquired immunodeficiency syndrome in that population. over the past 3 years we have seen 16 examples of a lesion that histologically resembles hairy leukoplakia but is found in patients who are not in risk groups for acquired immunodeficiency syndrome. all these specimens tested negative for epstein-barr virus dna and for human papillomavirus antigen ... | 1989 | 2542860 |
| the expression of histocompatibility antigen hla-dr in cervical squamous epithelium infected with human papilloma virus. | uterine cervices with histologic changes suggestive of human papilloma virus (hpv) infection were examined for the presence of papilloma virus capsid antigens and the class ii histocompatibility antigen hla-dr. the purpose of this study was to determine whether papilloma virus infection could induce hla-dr expression by squamous cells. this expression would allow squamous epithelium to function as antigen-presenting cells and perhaps initiate the immune response. in 20 cases in which hpv capsid ... | 1989 | 2542929 |
| detection and localization of human papillomavirus in penile condylomas and squamous cell carcinomas using in situ hybridization with biotinylated dna viral probes. | human papillomavirus (hpv) has been previously demonstrated in male genital neoplasms using southern blot hybridization (sbh) and in situ hybridization with radiolabeled probes (ish-r). in this study we used in situ hybridization with biotinylated dna viral probes (ish-b), a technique that can be applied to routinely collected and processed tissue. thirty cases of exophytic penile condyloma acuminatum and nine cases of invasive squamous cell carcinoma of the penis were examined for the presence ... | 1989 | 2542932 |
| human papilloma virus type 11 in a fatal case of esophageal and bronchial papillomatosis. | we report a case of an apparently healthy 27-year-old man with a fatal course of papillomatosis, originating in the distal part of the esophagus and spreading into the main and intermediate bronchus. human papillomavirus type 11, usually associated with juvenile laryngeal papillomatosis and genital condyloma acuminatum, was detected in the papillomas. in spite of treatment with co2-laser evaporation of the papillomas, and with systemic as well as topical interferon, vp-16 and bleomycin, the papi ... | 1989 | 2543063 |
| cytologic and ultrastructural findings of a peculiar alteration in cervical cells from patients with human papillomavirus infections. | inclusion bodies were previously found in papanicolaou smears of patients infected by the human papillomavirus (hpv). to ascertain their origin, 12 biopsies from patients with colposcopic evidence of papillomatous lesions were studied by cytology and electron microscopy. in several instances, koilocytotic atypias and electron-dense masses included within epithelial cells were observed. some of these epithelial cells appeared to be surrounded by other cells in a sort of concentric arrangement. th ... | 1989 | 2543170 |
| human papillomavirus infection. frequency and association with cervical neoplasia in a young population. | one million, six hundred thirty-two thousand, eight hundred forty-seven women from two independent populations in the united states received cytologic screening during a two-year period. condylomatous lesions (human papillomavirus [hpv] infections) were the most frequent cytologic abnormality in women in both the planned parenthood and private sector groups (prevalence rates of 18.6 to 19.0 in women between ages 15 to 19). the prevalence rates of mild-to-moderate dysplasia were also similar in b ... | 1989 | 2543171 |
| the frequency of human papillomavirus infections, with or without associated cervical intraepithelial neoplasia, in cervicovaginal smears. | 1989 | 2543174 | |
| human papillomavirus (hpv) infection in seborrheic keratosis. | we studied 89 seborrheic keratoses. in four cases, light microscopy and electron microscopy demonstrated that seborrheic keratoses may be associated with human papillomavirus (hpv) infection. | 1989 | 2543229 |
| [study of the clinical background and therapeutic method in patients with vulvar cancer]. | forty-two cases of vulvar cancer were reviewed with regard to clinical condition and therapeutic method, especially the efficacy of local administration of brm. by histological examination, koilocytosis was found in 45%. furthermore, there was 90% positive koilocytosis rate in immunohistological study using anti-human papilloma virus antibody and in situ hybridization method by hpv dna probe (type 6, 11, 16, 18). the hpa was mostly type 16. therefore, vulvar cancer was considered to occur in clo ... | 1989 | 2543316 |
| [human papillomavirus dna in vulvar lesions]. | in order to study the role of human papillomavirus (hpv) in genital cancer, we attempt to determine the presence of hpv in tumors of the genital tract. in the current study, we have examined 35 histologically diagnosed cases of vulvar lesions in attempts to compare the sensitivities of various methods of detecting hpv. all but 7 vulvar lesions contained hpv dna by southern blot dna hybridization. detectability of hpv by immunocytochemistry and koilocytosis significantly declined as compared to d ... | 1989 | 2543317 |
| molecular characteristics and physical state of human papillomavirus dna change with progressing malignancy: studies in a patient with epidermodysplasia verruciformis. | in order to establish the role of human papillomavirus (hpv) in carcinogenesis of epidermodysplasia verruciformis (ev), the presence, the molecular characteristics and the physical state of hpv dna in a benign lesion, a primary carcinoma and a metastatic carcinoma developing in the same ev patient were studied and compared. of the 2 hpv dnas isolated from benign macular lesions, only one (a subtype of hpv 5) was detected in both primary and metastatic tumors. only one normal species of viral dna ... | 1989 | 2543640 |
| human papillomavirus infection and anal cancer. | to study the association of human papillomavirus (hpv) infection with anal cancer, we examined tissue specimens from 126 patients with malignant lesions of the anal skin or mucosa. the patients were enrolled in a population-based, case-control study of ano-rectal cancer which is being conducted in the state of washington and the province of british columbia. histologic sections from formalin-fixed, paraffin-embedded tissues were tested for the presence of hpv dna by in situ hybridization with bi ... | 1989 | 2543642 |
| [in situ hybridization study on the localization of hpv dna in the precancerous lesions of uterine cervix]. | human papillomavirus (hpv) types 16 and 18 have been found closely associated with cervical cancer. in order to investigate the relationship between hpv dna and cervical precancerous lesions, we examined the formalin fixed specimens obtained from 22 cases of mild dysplasia, 33 cases of moderate dysplasia and 31 cases of severe dysplasia of the uterine cervix for the presence of hpv 6/11, 16 and 18 dnas by in situ hybridization using the biotinylated hpv dna probes. we also followed some hpv dna ... | 1989 | 2543717 |
| reactivities of polyclonal and monoclonal antibodies raised to the major capsid protein of human papillomavirus type 16. | polyclonal and monoclonal antibodies have been raised against a fusion protein containing beta-galactosidase and part of the major capsid protein l1 of the human papillomavirus (hpv) type 16. the polyclonal antibodies cross-reacted with the l1 protein of several hpv types including hpv-1, -2, -6 and -11 when reacted with virus-infected tissue sections, and with hpv-6 and -18 l1 fusion proteins on western blotting. monoclonal antibodies against the l1 fusion protein of hpv-16 reacted only with hp ... | 1989 | 2543739 |
| human papillomavirus types 6 and 16 in cooperation with ha-ras transform secondary rat embryo fibroblasts. | using a focus assay we have shown that the entire human papillomavirus type 16 (hpv-16) genome is capable of cooperating with an activated ras oncogene to transform secondary rat embryo fibroblast (ref) cells as indicated by focus formation of unselected cells. however, this assay failed to detect any similar activity with either the whole hpv-6 genome or with subgenomic fragments. in contrast, transformed colonies appeared when g418 (geneticin)-resistant colonies were selected after cotransfect ... | 1989 | 2543741 |
| identification of papillomaviral dna sequences in hairless mouse tumours induced by ultraviolet irradiation. | papillomas, carcinomas in situ and squamous cell carcinomas were induced using ultraviolet irradiation in the hairless mouse strain mus musculus hra/skh. dna extracted from biopsies was examined using mastomys natalensis papillomaviral dna as a hybridization probe at reduced stringency. sequences homologous to the probe were detected in 16 of 24 papillomas, five of five carcinomas in situ and six of 38 squamous cell carcinomas. a number of tumour dnas (16/33) also hybridized with mixed dnas of h ... | 1989 | 2543760 |
| a novel deletion within the upstream regulatory region of episomal human papillomavirus type 16. | the pattern of human papillomavirus type 16 (hpv-16) integration was studied in 23 invasive carcinomas of the cervix using subgenomic probes. seventeen tumours contained integrated hpv-16 and in 13 of these there was evidence of disruption within the e1-e2 open reading frames (orfs). in all cases the upstream regulatory region (urr)-e6-e7 orfs was maintained intact. two independently derived tumours were infected with episomal wild-type hpv-16 and an episomal variant of hpv-16 containing a 325 b ... | 1989 | 2543768 |
| immortalization of primary human epithelial cells by cloned cervical carcinoma dna containing human papillomavirus type 16 e6/e7 open reading frames. | primary human epithelial cells were transfected with a subgenomic fragment of dna cloned from a cervical carcinoma, containing the putative transforming genes e6 and e7 from the human papillomavirus type 16 prototype. several immortalized cell lines were generated, all retaining tumour dna and expressing the viral genes, suggesting that either one or both of these genes is sufficient for the immortalization of primary human genital keratinocytes. | 1989 | 2543780 |
| presence of human papillomavirus type 18 dna in vulvar carcinomas and its integration into the cell genome. | we have screened 78 genital tract tumours from italian female patients for the presence of human papillomavirus type 16 (hpv-16) and hpv-18 dna. dot and southern blot hybridization experiments revealed that dna sequences of hpv-16 and/or hpv-18 are present in 66% of tumour samples. hpv-18 was detected in 80% of vulvar carcinomas. in these tumours the integration of hpv-18 dna seems to occur in the e1/e2 region of the virus genome with long deletions in almost all samples. the invariable retentio ... | 1989 | 2543791 |
| the multicentric nature of disease related to human papillomavirus infection of the female lower genital tract. | 1989 | 2543936 | |
| [detection of papillomaviruses by in situ hybridization with sulfonated probes]. | a technique of detection by in situ hybridization of human papillomavirus in sections of condylomatous lesions is described. the probes are labeled and modified by sulfonation and the hybrids are revealed by immunohistochemistry, using alkaline phosphatase. | 1989 | 2544192 |
| detection of human papillomavirus genes in human oral tissue biopsies and cultures by polymerase chain reaction. | we have used the polymerase chain reaction to detect dna sequences related to human papillomavirus type 16, by simultaneous priming with oligonucleotides from the e6 and l1/l2 open reading frames of the hpv16 genome. the hpv16-related sequence is present at low levels in normal oral tissue, in addition to biopsies and cell cultures from patients with benign and malignant disease. ultimate analysis of the amplified sequences from the e6(120bp) and l1/l2(173bp) regions of hpv16 was achieved by gel ... | 1989 | 2544219 |
| human papillomavirus (hpv)-associated male and female genital carcinomas in a hindu population. the male as vector and victim. | epidemiologic, histologic and immunohistochemical data concerning male (penile) and female (cervical and vulvar/vaginal) genital carcinomas in a hindu population are reported. the data are from bali, an indonesian hindu island in a country with a predominantly muslim population. in contrast to the surrounding muslim population, circumcision is rare in the people of bali, and the rate of phimosis in grown men is very high. the balinese epidemiologic data of 1985 to 1986 were compared with 1986 da ... | 1989 | 2544257 |
| genital human papillomavirus infection. | 1989 | 2544328 | |
| virology of human papillomavirus. | 1989 | 2544331 | |
| genital human papillomavirus infection. management strategies. | 1989 | 2544338 | |
| interphase cytogenetics using biotin and digoxigenin labelled probes i: relative sensitivity of both reporter molecules for detection of hpv16 in caski cells. | this study was undertaken to develop technology for the detection of nucleic acid using two different dna probe reporter molecules, the ultimate aim being to differentially label two nucleic acids within the same nucleus. digoxigenin and biotin were used to label dna probes. the absolute and relative sensitivity of digoxigenin and biotin labelled dna probes for detecting integrated human papilloma virus 16 (hpv16) was investigated in caski cells by non-isotopic in situ hybridisation (nish). seve ... | 1989 | 2544632 |
| interphase cytogenetics using biotin and digoxigenin labelled probes ii: simultaneous differential detection of human and papilloma virus nucleic acids in individual nuclei. | a method was developed for the simultaneous detection of viral and human dna in contrasting colours in routine formalin fixed, paraffin wax embedded biopsy specimens. this was achieved by non-isotopic in situ hybridisation (nish) with a biotinylated y chromosome probe and digoxigenin labelled probe for human papilloma virus type 6 (hpv 6). the tissues studied were peripheral lymphocytes, tonsil, and penile warts. the hybridisation signals produced by biotinylated probes were visualised in red us ... | 1989 | 2544633 |
| structure and expression of an integrated human papillomavirus type 16 genome amplified in a cervical carcinoma cell line. | a cellular sequence containing the integrated human papillomavirus type 16 genome in a cervical carcinoma cell line qg-u was cloned and analysed. the transcriptionally active viral genome disrupted at the e2 and l2 open reading frames was amplified with its flanking sequences. | 1989 | 2544671 |
| loss of leukoregulin up-regulation of natural killer but not lymphokine-activated killer lymphocytotoxicity in human papillomavirus 16 dna-immortalized cervical epithelial cells. | the sensitivity of human cervical epithelial cells immortalized by transfection with human papillomavirus type 16 (hpv16) dna, to lysis by natural killer (nk) and lymphokine-activated killer (lak) lymphocytes was evaluated at progressive stages of transformation. both early- (10-20 wk) and late- (greater than 30 wk) passage hpv16-immortalized cells were resistant to nk lymphocyte cytotoxicity but sensitive to lak lymphocyte cytotoxicity at lymphocyte-to-cervical cell ratios ranging from 1:1 to 5 ... | 1989 | 2544741 |
| ameloblastoma in young persons: a clinicopathologic analysis and etiologic investigation. | ameloblastoma, an odontogenic tumor of ectodermal origin, has been reported to arise, on rare occasions, in a primordial or dentigerous cyst of a young person. numerous authors have suggested differing nomenclatures for these ameloblastomas (e.g., mural, unicystic, monocystic, intracystic, cystogenic, cystic, plexiform unicystic) and have sought to describe and classify the clinical and histopathologic features. these tumors have been characterized as a distinct variant exhibiting less aggressiv ... | 1989 | 2544844 |
| [cervicovaginal and vulvar papillomavirus lesions: epidemiology in hiv seropositive women. a preliminary study on a continuous series]. | in a series of 29 hiv-seropositive women (mean age 27.4 years; 75.9 per cent acquired hiv by intravenous drug abuse) observed over a 2 years' period, we were able to determine the main epidemiological characteristics of cervico-vaginal and vulvar lesions caused by human papillomavirus (hpv). more than 58 per cent of cervico-vaginal smears showed cytological evidence of hpv infection (koilocytosis), and 6 cervical biopsies showed histological abnormalities (from cin i to invasive carcinoma). cond ... | 1989 | 2544871 |
| two newly identified human papillomavirus types (hpv 40 and 57) isolated from mucosal lesions. | two new papillomaviruses, hpv 40 and hpv 57, were isolated from a pin lesion and an inverted papilloma of the maxillary sinus, respectively. hpv 40 showed a 13% homology to hpv 7 by reassociation kinetics and hpv 57 showed a 17% homology to hpv 2 and 25% homology to hpv 27. hybridization of the dna of these papillomaviruses to a wide variety of different tumor biopsies revealed that hpv 40 was present in a few genital condylomata acuminata as well as in bowenoid lesions. hpv 57 dna was present i ... | 1989 | 2545031 |
| characterization of an hpv type 11 isolate propagated in human foreskin implants in nude mice. | human papillomavirus type 11-hershey (hpv-11-h) from an extract of genital warts has been serially passaged by infecting human foreskin chips that are then implanted in athymic mice (j. w. kreider, m. k. howett, a. e. leure-dupree, r. j. zaino, and j. a. weber (1987), j. virol. 61, 590). subsequent attempts to propagate hpvs present in other human lesions have not been successful. in an effort to identify the basis for the seemingly unique ability of hpv-11-h to propagate in the xenografts, we c ... | 1989 | 2545034 |
| nucleotide sequence of human papillomavirus type 31: a cervical neoplasia-associated virus. | the nucleotide sequence of human papillomavirus (hpv) 31 dna (7912 bp) was determined and used to deduce the genomic organization of this cervical cancer-associated virus. based on comparisons of the hpv 31 dna sequence to other sequenced hpvs, hpv 31 is a typical papillomavirus most related to hpv 16 (70% identical nucleotides). the e6 and e7 open reading frames (orf) of hpv 31 contain several potential dna binding motifs (cys-x-x-cys), the locations of which are conserved in all hpvs. the e6 o ... | 1989 | 2545036 |
| condylomatous lesion (hpv infection) of uterine cervix. a clinical, colposcopic, cyto, histomorphological and immunohistochemical study. | 43, patients, subjected to cervical cytology, colposcopy and colposcopic directed biopsy, were found to have lesions suggestive of human papillomavirus (hpv) infection by one or more parameters. immunohistochemical staining for hpv antigen yielded positive results in 23 (53.5 p. cent) cases. amongst 30 cases with hpv lesions both in cytology and histopathology, 19 (63.3 p. cent) were positive for the hpv antigen. this was significantly higher as compared to the groups where hpv lesion was observ ... | 1989 | 2545120 |
| [warts and epidermoid carcinoma after renal transplantation]. | kidney transplant recipients suffer in the long-term from several cutaneous disorders linked to the transplantation. we had the opportunity to observe several patients presenting with pre-epitheliomatous keratoses and cutaneous carcinomas associated with warts. we report herein on five cases that were subjected to a clinical, histological and virological study. material and methods. clinical and histological report. the patients were referred to use by the kidney transplantation department of th ... | 1989 | 2545155 |
| amplification of the integrated viral transforming genes of human papillomavirus 18 and its 5'-flanking cellular sequence located near the myc protooncogene in hela cells. | the human papillomavirus type 18 integrated in the hela cell genome is amplified on chromosome 8. e6, e7, and e1 open reading frames are amplified 5-fold, and the late viral dna region, the viral long control region, and cellular flanking sequences are amplified 15-fold. the common 5'-flanking cellular dna was localized by in situ hybridization of normal and hela cells only on chromosome 8 band q24. this flanking probe is included in the amplification unit of colo320 cells, but in the case of he ... | 1989 | 2545339 |
| human papilloma virus infection and its relationship to skin cancer in a group of renal allograft recipients. | 1989 | 2545416 | |
| infectious papillomavirus in the vapor of warts treated with carbon dioxide laser or electrocoagulation: detection and protection. | papillomavirus dna has been reported recently in the vapor (smoke plume) derived from warts treated with carbon dioxide laser; this raises concerns for operator safety. we therefore have studied a group of human and bovine warts to define further the potential risk of wart therapy and to test whether a surgical mask could reduce exposure. half of each wart was treated with carbon dioxide laser and the other half with electrocoagulation. the vapor produced by each form of therapy was collected wi ... | 1989 | 2545749 |
| human papillomavirus type 31 infection of the uterine cervix in england. | a total of 452 uterine cervical scrapes, from two centres in england, has been examined by dot blot hybridisation for the presence of human papilloma virus (hpv) types 6, 11, 16, 18 and 31. hpv dna was found in 36.5% samples. twenty-nine of the women were infected with more than one type. of those with detectable hpv, 14.5% had hpv type 31 infections. this type appears to cause significant infection of the genital tract in england. | 1989 | 2545788 |
| oral hairy leukoplakia is not a specific sign of hiv-infection but related to immunosuppression in general. | oral hairy leukoplakia (hl) has been regarded as an early sign of hiv infection, and its clinical importance related to the poor outcome of the patients has been emphasized. initially, hl was observed exclusively among male homosexuals, but subsequently demonstrated in all risk groups of hiv infection. the patient described in this article suggests that oral hl is not specific for hiv infection per se, but may be associated with immunosuppression also due to other causes. we describe an hiv-sero ... | 1989 | 2545869 |
| progesterone and glucocorticoid response elements occur in the long control regions of several human papillomaviruses involved in anogenital neoplasia. | we have previously identified in the long control region of the genome of human papillomavirus type 16 (hpv-16) a dna segment which functions as a cell-type-specific enhancer as well as mediating glucocorticoid response. it contains multiple transcription-factor-binding sites, including several for nuclear factor i and one for the glucocorticoid receptor, which binds to the partially palindromic sequence tgtacannntgtcat. we report here that this sequence element, when separated from the surround ... | 1989 | 2545902 |
| [histopathological study on human papilloma virus (hpv) infection of the uterine cervix]. | 1989 | 2545952 | |
| association of dna aneuploidy with human papillomavirus-induced malignant transformation of sinonasal transitional papillomas. | the nuclear dna content of 19 transitional papillomas of the sinonasal region and 9 maxillary squamous cell carcinomas was studied by flow cytometry; the presence of human papillomavirus (hpv) dna types 11 and 16 was determined by the in situ hybridization technique from paraffin-embedded tissue. thirteen (68%) of the papillomas and none of the carcinomas contained hpv genome. six (32%) of the papillomas and 4 (44%) of the carcinomas had an aneuploid dna content. the relative dna content (dna in ... | 1989 | 2546115 |
| human papillomavirus dna determination of anal condylomata, dysplasias, and squamous carcinomas with in situ hybridization. | infection with types 6, 11, 16, and 18 of the human papillomavirus (hpv) is associated with condylomatous, dysplastic, or carcinomatous changes in the genital tract. emerging evidence suggests that a similar series of lesions develops in the anal canal after exposure to the same hpv types. in situ hybridization was performed with the use of biotinylated dna probes to hpv 6, 11, 16, and 18, so as to determine the frequency of hpv dna in 45 perianal and/or anal condylomata, 6 anal intraepithelial ... | 1989 | 2546419 |
| increased frequency of detection of human papillomavirus deoxyribonucleic acid in exfoliated cervical cells during pregnancy. | exfoliated cells of the uterine cervix obtained from women during pregnancy and at the time of their first postpartum examination were used to monitor the prevalence of human papillomavirus infections in this population and to study the natural fluctuations in viral expression. when deoxyribonucleic acid hybridization analysis alone was used to monitor the presence of human papillomavirus infection, 20.9% of our study population had results that were positive for human papillomavirus deoxyribonu ... | 1989 | 2546428 |
| mechanism for human papillomavirus transmission at birth. | we attempted to investigate mechanisms, in addition to sexual contact, by which human papillomaviruses associated with anogenital tract lesions could be transmitted. samples of exfoliated cervical cells were obtained from 45 pregnant women and were assayed by southern blot hybridization analysis for the presence of human papillomavirus nucleic acids. twenty-five of the 45 women had cells positive for human papillomavirus deoxyribonucleic acid. a neonatal nasopharyngeal aspirate was obtained at t ... | 1989 | 2546429 |
| correlation of histology and human papillomavirus dna detection in condyloma acuminatum and condyloma-like vulvar lesions. | the diagnosis of a vulvar condyloma is made when perinuclear halos are seen with nuclear atypia and binucleate forms (koilocytotic atypia). these changes are most prominent in the granular layer and are associated with the presence of human papillomavirus (hpv). however, these changes may be absent or minimal in patients with papillary vulvar lesions; this situation can thus present diagnostic difficulties. we analyzed the histologic features of 53 biopsies from 48 patients who had vulvar lesion ... | 1989 | 2546460 |
| the common occurrence of human papillomavirus infection and intraepithelial neoplasia in women infected by hiv. | eighteen out of 19 women who were clinically well but infected by hiv showed abnormalities of the lower genital tract. seven patients had intraepithelial neoplasia, histologically verified in five, involving the cervix in four, the vulva in three and the perineum in one. lesions (clinical and/or subclinical) compatible with human papillomavirus (hpv) infection were identified in these women, as well as in 10 out of the other 11 women infected with hiv. disease at more than one site was detected ... | 1989 | 2546572 |
| human papillomavirus and cervical cancer. | 1989 | 2546882 | |
| human papillomavirus and cervical cancer. summary report. | 1989 | 2546883 | |
| measurement error in epidemiological studies of human papillomavirus and cancer. | 1989 | 2546886 | |
| human papillomavirus and cervical neoplasia: a critical review of the epidemiological evidence. | 1989 | 2546887 | |
| human papillomavirus-related changes in the genital tract. | 1989 | 2546889 | |
| typing of human papillomaviruses in cervical intraepithelial neoplasia grade 3 biopsies from cape town. | dna from 98 cervical intraepithelial neoplasia grade 3 (cin 3) biopsies was screened, using the southern blotting hybridization technique, for human papillomavirus (hpv) types 6, 11, 16, 18, 31, 33, and 35. hpv 16 was detected in 16 biopsies (16%), hpv 33 in eight biopsies (8%), hpv 31 in two biopsies (2%), and hpv 18 in one biopsy (1%). one of the biopsies contained both hpv 31 and 18. six biopsies (6%) contained an hpv type very similar but not identical to hpv 16, and 35 biopsies were positiv ... | 1989 | 2547018 |
| topography of early hpv 16 transcription in high-grade genital precancers. | the extent to which human papillomavirus (hpv) type 16 is transcribed and the nature of the transcripts produced in genital precancers has not been clearly defined. the authors analyzed 28 cases of cervical (cin) or vulvar (vin) intraepithelial neoplasia by rna-rna in situ hybridization, using probes generated from hpv 16 open reading frames (orfs) either upstream (e6-e7) or downstream (e2-e5-l2) to the e1 orf, where hpv 16 genomic integration most commonly occurs. hybridization signals correspo ... | 1989 | 2547317 |
| the male factor in the etiology of cervical cancer among sexually monogamous women. | to address the hypothesis that male sexual behavior may affect the etiology of invasive cervical cancer, a case-control study was undertaken in panama, costa rica, colombia and mexico. the study enrolled husbands of those women with invasive cervical cancer and of those age-matched controls who reported only one lifetime sexual partner. a total of 204 case and 485 control husbands (78% and 72%, respectively, of identified husbands) were interviewed, clinically examined, and had penile swabs take ... | 1989 | 2547727 |
| human cervical carcinoma cell lines contain an antigen identical to the tumor-specific 75 kda antigen of hela cells: detection by viral acquisition. | purified vesicular stomatitis virus grown in the human cervical carcinoma hela cell line, vsv(hela), contains a 75 kda tumor-specific antigen, detectable by immunoblotting of electrophoretically separated proteins with rabbit antiserum made against whole hela cells. nearly identical results were obtained with vsv grown in the tumorigenic human hybrid esh-5l cells, but not with the matched non-tumorigenic esh-5e cells. growth of vsv in 4 other independently isolated human cervical carcinoma cell ... | 1989 | 2547812 |
| laryngeal papillomatosis: correlation between severity of disease and presence of hpv 6 and 11 detected by in situ dna hybridisation. | a technique using a biotin-streptavidin polyalkaline phosphatase complex was applied to routinely fixed and processed biopsy specimens of laryngeal papillomata from 45 patients taken over the past 20 years to detect human papilloma virus (hpv) types 6 and 11. two thirds of both adult and juvenile onset cases were positive for hpv 6 or hpv 11 or both. five specimens of normal vocal cord epithelium were negative for hpv 6 and 11. the detailed clinical history, endoscopic findings, success of treat ... | 1989 | 2547844 |
| comparison of human papillomavirus type 1 serotyping by monoclonal antibodies with genotyping by in situ hybridization of plantar warts. | thirty plantar warts were analyzed for the presence of hpv-1 type-specific and pv genus-specific capsid antigens by immunofluorescence (if) using monoclonal and polyclonal antibodies and type-specific hpv-1 dna employing in situ hybridization methods. fifteen of 30 plantar warts were positive by if for pv genus-specific structural viral antigens. thirteen of the 15 productively infected plantar warts expressed intranuclear hpv-1 type-specific capsid antigens and viral dna, which were detected in ... | 1989 | 2547854 |
| detection of human papillomavirus (hpv) dna in focal epithelial hyperplasia. | five focal epithelial hyperplasia (feh) specimens from four patients were examined by southern blot hybridization analysis to determine the specific human papillomavirus (hpv) types present. the histomorphologic features of these specimens were also evaluated and a broad variety of changes including koilocytes, mitosoid cells, ballooning cells and cells showing individual cell keratinization were noted. feh lesions from the three patients sharing a familial relationship demonstrated hpv dna sequ ... | 1989 | 2547947 |
| a new and sensitive method of screening for human papillomavirus infection. | human papillomaviruses (hpvs) have been implicated in the development of cervical cancer, and hpv screening may one day supplement cytologic analysis of cervical samples. detection of the small amounts of hpv dna in cervical scrapes has been very difficult. the polymerase chain reaction is a new technique that can specifically amplify target dna to facilitate its detection. we have amplified a region of hpv type 16 dna using this technique and have shown the method to be both specific and sensit ... | 1989 | 2548136 |
| detection of human papillomavirus dna in cervical lesions by in-situ dna hybridization. | authors examined paraffin sections of 50 cervical specimens from 34 cases for the presence of human papillomavirus (hpv) type 6b, 11, 16, 18, 31 and 33 by in-situ hybridization using 35s-labelled hpv dna probes. specimens were classified according to the degree of dysplasia after histological examination. viral nucleic acids were detected in 30 of 50 tissues (60%) in which 15 specimens had single, 10 double, 4 triple and 1 quadruple viral infections. in some cases, different viral nucleic acids ... | 1989 | 2548149 |
| constitutive expression of c-myc oncogene confers hormone independence and enhanced growth-factor responsiveness on cells transformed by human papilloma virus type 16. | the effect of constitutive expression of the c-myc oncogene on the biological properties of cells transformed or immortalized by human papilloma virus type 16 (hpv16) was studied. whereas transfection of hpv16 alone into primary baby mouse kidney (bmk) cells failed to generate any immortalized cell lines unless the tumor promoter phorbol 12-myristate 13-acetate was present, cotransfection of hpv16 with a plasmid that constitutively expresses murine c-myc (psvc-myc-1) generated numerous rapidly g ... | 1989 | 2548186 |
| [ultrastructural demonstration of the human papilloma virus (hpv) in oral proliferative lesions]. | the human papilloma virus (hpv) has been found in a series of benign proliferative lesions of the skin and mucosa. the virus has also been found in verrucous laryngeal carcinoma and carcinomas of the oral cavity and other organs. dna hybridization techniques have made it possible to classify 51 types of hpvs, some appearing to be associated with specific lesions. in order to study the intracellular distribution of hpvs, an ultrastructural morphological analysis was performed with an electron mic ... | 1989 | 2548368 |
| chromatin structure and transcriptional regulation of human papillomavirus type 18 dna in hela cells. | mapping analysis of the nucleosomal organization of integrated human papillomavirus type 18 (hpv18) dna in hela cells reveals a very prominent nuclease-hypersensitive site within the viral noncoding regulatory region that harbors transcriptional control sequences and coincides with most of the 5' ends of the cytoplasmic early mrnas. moreover, it is shown that the conserved coamplified 5' cellular flank, common to all hpv18 copies in hela cells and located close to the virus-cell integration site ... | 1989 | 2548528 |
| human papillomavirus and epithelial ovarian neoplasia. | in contrast to the strong association between human papillomavirus (hpv) and squamous cell carcinoma of the lower female genital tract, no viral dna had been found in epithelial ovarian carcinoma. recently, however, r. h. kaufman, j. bornstein, a. n. gordon, e. adam, a. l. kaplan, and k. adler-storthy [gynecol. oncol. 27, 340-349 (1987)] reported the detection of hpv-6 dna by in situ hybridization in 10 of 12 patients with advanced epithelial ovarian carcinoma. to further investigate the possibl ... | 1989 | 2548934 |
| [the study on in vitro transformation by human papillomavirus type 16 e6/e7 region]. | a nih3t3-derived cell clone (na7) in which human papillomavirus (hpv) 16 early region e6/e7 was inducible by dexamethasone (dxm) under the control of mouse mammary tumor virus long terminal repeat was established. a transforming function for hpv 16 e6/e7 region was analyzed by this established clone. northern blot hybridization demonstrated that the increased expression of pcna/cyclin and c-myc at the confluent state in accordance with the induced expression of e6/e7 region by dxm. although comp ... | 1989 | 2548943 |
| human papillomavirus detection in urine samples from male patients by the polymerase chain reaction. | human papillomavirus (hpv) detection was performed using the polymerase chain reaction technique on urine samples from 17 male patients with condylomata acuminata in the meatus urethrae. urine samples from 14 male laboratory volunteers were analyzed as controls. the dna was extracted and purified from urine sediments, centrifuged at 1,800 and 100,000 x g, and subjected to 40 cycles of amplification with hpv 6 and hpv 11 type-specific anticontamination primers and the heat-stable taq dna polymera ... | 1989 | 2549095 |
| [fundamental and clinical studies on human papillomavirus infection in the uterine cervix especially by vira pap (dot blot) method]. | female genital organs infected with human papillomavirus (hpv) have drawn attention as std and in connection with the mechanism of carcinogenesis. recently, we used a simplified hpv detection kit, vira pap method in clinical tests. to estimate the usefulness of the vira pap method in clinical application, a comparison was made between the results of the vira pap method and those of the conventional method. furthermore, an investigation was made of the relationship between uterine-cervical lesion ... | 1989 | 2549133 |
| oestrogen stimulates differential transcription of human papillomavirus type 16 in siha cervical carcinoma cells. | human papillomavirus (hpv) type 16 is highly associated with cervical cancer, but it seems that cofactors such as hormones affect its potential oncogenicity. we have analysed the hpv-16 gene expression in response to sex hormones and glucocorticoids in siha cells, a human cervical carcinoma cell line. an eightfold induction of hpv-16 transcripts was obtained in oestrogen-treated siha cells. of the five hpv-16 transcripts detected in these cells only the two major ones, the 4.6 kb and the 4.1 kb ... | 1989 | 2549190 |