Publications

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studies on some helminths of the domestic pig (sus scrofa domestica) in western uttar pradesh. 196214011347
observations on a close association between glossina tachinoides and domestic pigs near nsukka, eastern nigeria. i.-trypanosoma congolense and t. brucei infections in the pigs. 196314042653
a strain of trichinella spiralis from kenya of low infectivity to rats and domestic pigs. 196314053279
observations on a close association between glossina tachinoides and domestic pigs near nsukka, eastern nigeria. ii. ecology and trypanosome infection rates in g. tachinoides. 196414147662
the skin of the domestic pig. 196414209446
studies on the comparative susceptibility of wild european and domestic swine to hog cholera. 196414266857
[contribution to the phylogenesis of the course of the coronary arteries to the myocardium in domestic swine (sus scrofa domesticus)]. 196414266953
[on the developmental degree of the coronary vessel pattern in domestic swine (sus scrofa domesticus)]. 196414266954
studies on the urinary excretion of 17-ketosteroids by the domestic pig. 196514268459
[7 cases of hyperdactylia of the thoracic extremity in sus scrofa domestica l]. 196414279656
a hyper-parasite of iodamoeba buetschlii from the rhesus monkey, macaca mulatta, and the domestic pig. 196514292556
surface tension of the lung: the effect of bronchopulmonary denervation in the domestic pig. 196514292941
[fibrillar architecture of the epidermis and sinus hair of the snout of the domestic pig (sus scrofa domesticus), detected by polarization]. 195514387122
[leptospiroses of domestic swine and their significance for the epidemiology of human leptospiroses]. 195914423129
syphacia srivastav n.sp. from domestic pig in india. 196014447123
chest wall disruption with and without acute lung injury: effects of continuous positive airway pressure therapy on ventilation and perfusion relationships.we investigated the evolution of lung injury in an animal model with multiple rib fractures, both with and without acute lung injury, and the influence of spontaneous breathing with continuous positive airway pressure (cpap) therapy on the relative distributions of alveolar ventilation ([ov0312]a) and perfusion ([ov0422]).200314501968
intake of nutrients from pasture by pigs.the domestic pig evolved from the omnivorous wild boar and has retained many natural foraging behaviour patterns. although most modem commercial pigs are housed and fed concentrate diets, interest in the potential nutrient contribution from pasture has increased due to growth in outdoor and organic pig production systems. the energy requirements of outdoor pigs are increased by approximately 15% under northern european conditions as a result of greater climatic energy demand and locomotory activ ...200314506873
basic fibroblast and epidermal growth factors stimulate survival in adult porcine photoreceptor cell cultures.to investigate the effects of basic fibroblast and epidermal growth factor (fgf2 and egf, respectively) on the survival and phenotypic expression of photoreceptors isolated from adult mammalian retinas.200314507904
mitochondrial dna sequence variation and phylogenetic relationships among iberian pigs and other domestic and wild pig populations.nucleotide sequences of mitochondrial dna (mtdna) cytochrome b gene (1140 bp) and control region (707 bp) were used to determine the phylogenetic relationships among 51 pig samples representing ancient and current varieties of iberian pigs (26), spanish wild boars (seven) and other domestic pigs (18) of cosmopolitan (duroc, large white, landrace, pietrain and meishan) and local (spotted black jabugo, basque and mangalitza) breeds. a neighbour-joining tree constructed from pairwise distances prov ...200314510666
experimental model of continent colostomy using rectus abdominis neosphincteroplasty.use of dynamic myoplasty to create a continent stoma has produced promising results, but long-term stoma continence has not been achieved. the aim of the study was to establish and test a new model.200314515299
genetic typing of recent classical swine fever virus isolates from croatia.during a period of 5 years (1997-2001) several outbreaks of classical swine fever (csf) were recorded in croatia. for genetic typing, fragments of 150 nucleotides within the 5'-non-translated region (5'-ntr) and 190 nucleotides within the e2 glycoprotein coding gene of nine field isolates that were derived from domestic pigs and wild boars were used. for better epizootiological understanding, isolates from other european countries were included in the study. the results show that the isolates be ...200314516705
increasing esophageal length: a comparison of laparoscopic versus transthoracic esophageal mobilization with and without vagal trunk division in pigs.new laparoscopic techniques allow both mediastinal mobilization and performance of a collis gastroplasty when necessary, and the utility of a transthoracic approach is questioned. the aim of this study was to compare the increase in esophageal length achievable with laparoscopic and transthoracic esophageal mobilization in pigs, and to assess the impact of vagal trunk division on esophageal length.200314522324
role of birds in transmission of classical swine fever virus.active transmission of classical swine fever virus (csfv) was studied in six birds (five ravens, one hooded crow) and two laying hens. cloacal swabs, blood and organs of birds and hens as well as blood and organ samples of pigs which had been fed with faeces derived from csfv infected birds or which had come in contact with faeces of infected hens were negative for csfv. none of the animals seroconverted during the study. this result demonstrates that active virus transmission by these animals i ...200314535936
genotyping of clostridium perfringens isolated from domestic and exotic ruminants and swine.clostridium perfringens types a, b, c, d and e are known to cause severe enteritis/enterotoxaemia and diseases (especially caused by type a) belonging to the gas oedema complex in many species. samples from the small intestine as well as faeces of domestic and exotic animals suffering from enterotoxaemic signs or having died within days after first occurance of toxaemia were submitted for typing c. perfringens toxovars by multiplex pcr. the following species have been investigated: domestic shee ...200314535937
influence of radiographic contrast media on myocardial tissue oxygen tension: nacl-controlled, randomised, comparative study of iohexol versus iopromide in an animal model.it was tested whether a bolus injection of 10 ml radiographic contrast medium (iopromide vs. iohexol), compared to a 10 ml nacl bolus and administered into the left anterior descending artery (lad) of farm pigs, influenced the tissue po2 in the territory of this artery. the radiographic contrast media and the nacl bolus were given in randomised order. the mean po2 lad fell from initially 40.3+/-10.9 mmhg to a minimal value of 22.5+/-8.9 mmhg 241+/-44 sec after injecting the iopromide bolus, with ...200314561904
spinal blood flow in 24-hour megadose glucocorticoid treatment in awake pigs.because of the controversy regarding the benefits of 24-hour administration of methylprednisolone in patients with spinal cord injury (sci), it is important to investigate its mechanism of action and side effects. this study was conducted to determine if high-dose methylprednisolone modulates neural and vertebral blood flow in an awake large-sized animal model without sci.200314563146
potential sites of virus latency associated with indigenous pseudorabies viruses in feral swine.free-ranging feral swine (sus scrofa) are known to be present in at least 32 states of the usa and are continuously expanding their range. infection with pseudorabies virus (prv) occurs in feral swine and the primary route of transmission in free-living conditions seems to be venereal. between 1995 and 1999, naturally infected feral swine and experimentally infected hybrid progeny of feral and domestic swine, were kept in isolation and evaluated for occurrence of latent prv indigenous to feral s ...200314567217
111in oxine labelled mesenchymal stem cell spect after intravenous administration in myocardial infarction.mesenchymal stem cells (mscs) have shown therapeutic potential if successfully delivered to the intended site of myocardial infarction. the purpose of this pilot study was to test the feasibility of 111in oxine labelling of mscs and single photon emission computed tomography (spect) imaging after intravenous administration in a porcine model of myocardial infarction. adult farm pigs (n=2) were subjected to closed chest experimental myocardial infarction. 111in oxine labelled mscs (1 x 10(7) to 2 ...200314569169
effects of the endothelin-1 receptor antagonist tezosentan on renal blood flow and diuresis during prolonged increased intra-abdominal pressure.it has earlier been shown that increased intra-abdominal pressure (iap) reduces renal blood circulation and urine output both clinically and experimentally. the aim of this study was to investigate the effect of endothelin-1 inhibition by the endothelin-1 receptor antagonist tezosentan on renal blood circulation and diuresis in pigs subjected to prolonged increased intra-abdominal pressure.200314572770
periocular injection of microspheres containing pkc412 inhibits choroidal neovascularization in a porcine model.oral administration of pkc412, a kinase inhibitor that blocks several isoforms of protein kinase c (pkc) and receptors for vascular endothelial growth factor (vegf), platelet-derived growth factor, and stem cell factor, inhibits ocular neovascularization in a murine model. the purpose of this study was to determine whether sustained local delivery of pkc412 in a human-sized eye inhibits choroidal neovascularization (cnv).200314578426
should we ventilate or cool the pulmonary graft inside the non-heart-beating donor?the ideal preservation method during the warm ischemic period in the non-heart-beating donor (nhbd) remains unclear. in this study we compare the protective effect of ventilation vs cooling of the non-perfused pulmonary graft.200314585384
interactions between tsetse and trypanosomes with implications for the control of trypanosomiasis.tsetse flies (diptera: glossinidae) are vectors of several species of pathogenic trypanosomes in tropical africa. human african trypanosomiasis (hat) is a zoonosis caused by trypanosoma brucei rhodesiense in east africa and t. b. gambiense in west and central africa. about 100000 new cases are reported per year, with many more probably remaining undetected. sixty million people living in 36 countries are at risk of infection. recently, t. b. gambiense trypanosomiasis has emerged as a major publi ...200314587696
the postnatal growth of the beta-cell mass in pigs.studies of the postnatal growth of the beta-cell mass in rats have revealed some unexpected and apparently paradoxical results, the most prominent being a beta-cell mass plateau in the early phase of life. we have studied the postnatal growth of the beta-cell mass in the domestic pig to investigate its development in a larger mammal. the pancreases from a total of 86 male pigs from 5 to 100 days of age were studied. the beta-cell mass increased linearly from day 5 to day 40, reached a plateau fr ...200314596676
vaccinology of classical swine fever: from lab to field.there are two types of classical swine fever vaccines available: the classical live and the recently developed e2 subunit vaccines. the live chinese strain vaccine is the most widely used. after a single vaccination, it confers solid immunity within a few days that appears to persist lifelong. the e2 subunit vaccine induces immunity from approximately 10-14 days after a single vaccination. the immunity may persist for more than a year, but is then not complete. the chinese strain vaccine may est ...200314599784
an investigation into the wound-healing properties of essential oil of ocimum gratissimum linn.to investigate the effects of ocimum oil and two antibacterial preparations, cicatrin (glaxowellcome) and cetavlex (astrazeneca), on the healing of full-thickness excisional and incisional wounds, created under anaesthesia, on the back of test and control groups of adult albino rabbits.200314601223
assessment of subacute inflammatory and proliferative response to coronary stenting in a porcine model by local gene expression studies and histomorphometry.the aim of the study was to analyse inflammatory and proliferative response early after coronary stenting by angiography, histomorphometry and local gene expression analysis using quantitative rt-pcr. therefore, eight german domestic pigs underwent stenting of the left coronary artery. selective coronary angiography was performed after 14 days. explanted coronary arteries were examined histomorphometrically after methacrylate-embedding. snap-frozen samples were examined for local gene expression ...200414615159
experimental infection of pregnant gilts with swine hepatitis e virus.to determine the effect of swine hepatitis e virus (hev) infection on pregnant gilts, their fetuses, and offspring, 12 gilts were intravenously inoculated with swine hev. six gilts, who were not inoculated, served as controls. all inoculated gilts became actively infected and shed hev in feces, but vertical transmission was not detected in the fetuses. there was no evidence of clinical disease in the gilts or their offspring. mild multifocal lymphohistiocytic hepatitis was observed in 4 of 12 in ...200314620868
evidence favoring the use of an alpha2-selective vasopressor agent for cardiopulmonary resuscitation.both alpha1- and beta-adrenergic agonists increase the severity of global myocardial ischemic injury. we hypothesized that combined beta- and alpha1-adrenergic blockade would improve initial resuscitation and postresuscitation myocardial and neurological functions. we further hypothesized that the resulting alpha2-actions of relatively brief duration would favor improved functions compared with the more prolonged effect of nonadrenergic vasopressin.200314623815
brain metabolism during cardiopulmonary resuscitation assessed with microdialysis.microdialysis is an established tool to analyse tissue biochemistry, but the value of this technique to monitor cardiopulmonary resuscitation (cpr) effects on cerebral metabolism is unknown. the purpose of this study was to assess the effects of active-compression-decompression (acd) cpr in combination with an inspiratory threshold valve (itv) (=experimental cpr) vs. standard cpr on cerebral metabolism measured with microdialysis.200314625117
immediate defibrillation versus interventions first in a swine model of prolonged ventricular fibrillation.we compared time-dependent rescue shock success when delivered immediately, to defibrillation preceded by 3 min of cpr, with and without high dose epinephrine (hde) in a swine model of prolonged ventricular fibrillation (vf). our hypotheses were that pretreatment with cpr and hde would produce higher rates of successful first-shock defibrillation and would prevent decay of the vf waveform, as measured by the scaling exponent (sce), when compared to immediate defibrillation. we also sought to det ...200314625118
epidemiology of mycobacterium bovis infections of pigs and wild boars using a molecular approach.a molecular epidemiological approach was applied to establishing a possible role for the wild boar as a natural reservoir of mycobacterium bovis in sierra de villuercas, western spain; an area free of farmed cattle and wild deer populations. spoligo and vntr typing were used over a three year period to study the epidemiological relationship between the occurrence of bovine tuberculosis (tb) in extensively bred iberian pigs and indigenous wild boar. the 37 sampled wild boar showed different degre ...200314637044
gene structure of pig sterol 12alpha-hydroxylase (cyp8b1) and expression in fetal liver: comparison with expression of taurochenodeoxycholic acid 6alpha-hydroxylase (cyp4a21).cholic acid is the major trihydroxy bile acid formed in most mammals. the domestic pig (sus scrofa) is an exception. the bile of adult pig is devoid of cholic acid whereas hyocholic acid is found in amounts equal to that of cholic acid in humans. the pathway leading to formation of hyocholic acid is believed to be species-specific and to have evolved in the pig to compensate for a nonexistent or deficient cholic acid biosynthesis. however, a high level of cholic acid has recently been found in t ...200314643796
[case report: porcine circovirus type 2 infection in an european wild boar (sus scrofa) in the state of brandenburg, germany].this case represents the first case of porcine circovirus type 2 (pcv-2)--infection in a free living european wild boar associated with morphological lesions, which are regarded as characteristic for postweaning multisystemic wasting syndrome (pmws) in domestic pigs. the animal, an approximately 10 month old male, was found dead in a rural area within the state of brandenburg, germany. the closest commercial pig farm is located in 3 km distance from the spot where the carcass was found. at necro ...200314650740
mosquito host-feeding patterns and implications for japanese encephalitis virus transmission in northern australia and papua new guinea.japanese encephalitis (je) virus spread to northern australia during the 1990s, transmitted by culex annulirostris skuse and other mosquitoes (diptera: culicidae). to determine the relative importance of various hosts for potential vectors of je virus, we investigated the host-feeding patterns of mosquitoes in northern australia and western province of papua new guinea, with particular attention to pigs, sus scrofa l. - the main amplifying host of je virus in south-east asia. mosquitoes were col ...200314651654
ropivacaine combined with various anti-arrhythmic drugs results in mild alterations in myocardial contractility in pigs.the present study was undertaken following the observation of a marked decrease in myocardial contractility after ropivacaine in a patient on amiodarone, in order to investigate the cardiovascular effects of combining ropivacaine with anti-arrhythmic drugs (aard).200314656782
atorvastatin stent coating does not reduce neointimal proliferation after coronary stenting.statins seem to be suitable for restenosis prevention via reduction of smooth muscle cell proliferation, anti-inflammatory effects, and improvement of endothelial function. the aim of the present study was to test the efficacy of an atorvastatin stent coating for restenosis inhibition in the porcine coronary stent model.200314663613
laparoscopic heminephrectomy using a new fibrin sealant powder.to determine the ability of a hemostatic fibrin sealant powder (hfsp), delivered laparoscopically, to achieve hemostasis and seal the collecting system during laparoscopic heminephrectomy.200314665378
wild boars (sus scrofa) as reservoirs of brucella suis biovar 2 in croatia.this work presents the results of findings for brucellosis in wild boars and domestic swine in two regions of croatia. in the region of djakovo the blood samples of 211 wild boars were analysed and in 29.4% of the samples serologically positive reactions were established. in the same region the blood samples of 1080 domestic swine on pastures were also analysed and positive serological reactions were established in 12.3%. in the regions around lonjsko polje the blood samples of 53 wild boars wer ...200314680058
description and evaluation of a ventriculo-coronary artery bypass device that provides bi-directional coronary flow.the objective of this study was to assess acute patency of a new myocardial revascularization device that connects the left ventricular cavity to a coronary artery (termed ventriculo-coronary artery bypass, vcab) thereby providing proximal and distal blood flow from the site of the anastomosis.200414690731
porcine wound models for skin substitution and burn treatment.skin regeneration is an important field of tissue engineering. especially in larger burns and chronic wounds, present treatments are insufficient in preventing scar formation and promoting healing. initial screening of potentially interesting products for skin substitution is usually done by in vitro tests. before entering the clinic, however, in vivo studies in immunocompetent animals are necessary to prove efficacy and provide information on safety aspects. we have obtained extensive experienc ...200414697858
spontaneous gasping generates cardiac output during cardiac arrest.to measure stroke volumes coincident with spontaneous gasping during untreated ventricular fibrillation and to evaluate the effects of gasping.200414707585
comprehensive evaluation of ureteral healing after electrosurgical endopyelotomy in a porcine model: original report and review of the literature.endopyelotomy (ep) has yet to equal the success achieved with open dismembered pyeloplasty. to understand better the ureteral response to ep we performed a timed histopathological evaluation of the porcine ureter after acucise (applied medical, rancho santa margarita, california) ep.200414713843
spatial comparison between wall shear stress measures and porcine arterial endothelial permeability.a better understanding of how hemodynamic factors affect the integrity and function of the vascular endothelium is necessary to appreciate more fully how atherosclerosis is initiated and promoted. a novel technique is presented to assess the relation between fluid dynamic variables and the permeability of the endothelium to macromolecules. fully anesthetized, domestic swine were intravenously injected with the albumin marker evans blue dye, which was allowed to circulate for 90 min. after the an ...200414715506
pcr identification of beef, sheep, goat, and pork in raw and heat-treated meat mixtures.a pcr assay has been developed for the specific and qualitative detection of pork (sus scrofa domesticus), beef (bos taurus), sheep (ovis aries), and goat (capra hircus) in raw and heat-treated meat mixtures. a forward common primer was designed on a conserved dna sequence in the mitochondrial 12s ribosomal rna gene (rrna), and reverse primers were designed to hybridize on species-specific dna sequences of each species considered. the different sizes of the species-specific amplicons, separated ...200414717369
efficacy of ultrasonic tissue dissector and tissue glue for laparoscopic partial nephrectomy in a porcine model.the purpose of this study was to evaluate the efficacy and long-term tissue effects of ultrasonic dissector and cyanoacrylate glue during laparoscopic partial nephrectomy in a porcine model. nine domestic pigs underwent laparoscopic left lower pole partial nephrectomy without vascular control. an ultrasonic tissue dissector was used to assist in the parenchymal incision, and the raw surfaces of the kidneys, including the exposed urinary system, were sealed with cyanoacrylate glue. no internal st ...200314717525
effects of statins on myocardial and coronary artery response to ischemia-reperfusion.this study tested the hypotheses that (i) lipophilic statins (atorvastatin and simvastatin) impair ventricular recovery from myocardial ischemia-reperfusion, owing to their greater myocyte permeability, compared with a hydrophilic statin (pravastatin), and (ii) statins enhance endothelium-dependent vasodilation of isolated coronary arteries from the ischemic region. farm pigs consumed chow supplemented with atorvastatin (2.5 mg.kg(-1).d(-1); n=6), pravastatin (10 (n=3) or 20 (n=2) mg.kg(-1).d(-1 ...200314719042
betaine improves growth, but does not induce whole body or hepatic palmitate oxidation in swine (sus scrofa domestica).dietary betaine may reduce carcass fat in growing pigs. we explored the effects of betaine on short-term growth and in vivo and in vitro fatty acid oxidation. pigs were housed in metabolism crates and fed diets containing either 0% (control), 0.125% or 0.5% betaine at 80% of ad libitum energy intake. fatty acid oxidation was measured during intravenous infusions of 1-(13)c-palmitate and in hepatocytes incubated in the presence or absence of betaine and carnitine. co2 and palmitate isotopic enric ...200414720598
functional and phylogenetic analyses of a melanocortin-4 receptor mutation in domestic pigs.the melanocortin-4 receptor (mc4r), a g protein-coupled seven-transmembrane receptor, which is expressed in the brain, plays an important role in the control of mammalian energy homeostasis. a missense mutation (asp298asn) was identified in the porcine mc4r gene, which is associated with growth and food intake traits. the asn298 mutation occurs within a highly conserved motif, npliy, of all members of g protein-coupled receptors; whereas, asp298 is conserved in all five melanocortin receptor sub ...200414732454
pressure-related hemodynamic effects of co2 pneumoperitoneum in a model of acute cardiac failure.the adverse effects related to co2 pneumoperitoneum (pp) have been well documented. although these effects in the state of cardiac failure have not been investigated, it appears to be common current clinical practice to use low-pressure pp in this clinical setting, assuming it to be safer.200314733695
a comparison of laparoscopic bipolar vessel sealing devices in the hemostasis of small-, medium-, and large-sized arteries.the development of new energy sources for hemostasis has facilitated advanced laparoscopic procedures. few studies, however, have documented the strength of the vessels sealed or the extent of surrounding lateral thermal injury, two important factors in maintaining hemostasis while preventing injury to surrounding structures. this study compared the burst pressure and extent of thermal injury of vessels sealed with the 5-mm laparoscopic plasmakinetics trade mark sealer (pk) (gyrus medical, maple ...200314733701
experimental disc degeneration due to endplate injury.the aim of this study was to create an experimental model of disc degeneration that closely mimicked human disc degeneration. in six domestic pigs, an l4 cranial endplate perforation into the nucleus pulposus was made. three months postoperatively, compressive testing was performed on the l2-l4 motion segments, and intradiscal pressure was measured in the intervening discs. histochemical and morphologic examinations were made on the excised degenerated and adjacent discs. a significant reduction ...200414734978
bipolar radiofrequency ablation of the kidney: comparison with monopolar radiofrequency ablation.we report initial ex vivo and in vivo studies using bipolar radiofrequency (rf) ablation of porcine kidneys. an internal ground electrode is positioned in the kidney opposite the rf electrode, resulting in ablation of all the intervening renal tissue.200314744366
long-term retention of endoscopically placed hydrogel prostheses at the lower esophageal sphincter in pigs.using flexible endoscopic methods, hydrogel prostheses can be safely placed, retained for long periods of time, and removed from the esophageal submucosa of pigs. this new technique may have future applications in the treatment of gastroesophageal reflux disease in selected situations.200414752644
[trichinellosis outbreaks in spain (1990-2001)].trichinellosis is a helminth infection having a wide geographical distribution. it is acquired by ingestion of raw or undercooked meat infected with trichinella spp. the present work reviews the human trichinellosis outbreaks recorded in spain during 1990-2001, taking into account both the geographical distribution and source of the infection.200414756987
how long can we preserve the pulmonary graft inside the nonheart-beating donor?the use of lungs from nonheart-beating donors (nhbd) might significantly alleviate the organ shortage. extending the preharvest interval in nhbd would facilitate distant organ retrieval. we hypothesized that prolonged topical cooling inside nhbd after 60 minutes of initial warm ischemia would not affect the pulmonary graft.200414759412
uptake of 111in-z2d3 on spect imaging in a swine model of coronary stent restenosis correlated with cell proliferation.small targets such as cell proliferation in the coronary arteries may potentially be detected with single-photon imaging using high-radiotracer-specific activity. we hypothesized that an antibody linked to polymers to increase specific radioactivity can be visualized on spect images and that counts in the target will correlate with the strength of the biologic signal.200414960651
do domestic pigs in controlled environments contrafreeload?many experiments report that animals will work (lever press) for food in the presence of freely available identical food. this phenomenon has attracted the attention of applied ethologists because it seems to prove that animals have a need to express appetitive behavior. if this is the case, then it has implications for the provision of environmental enrichment for animals in captivity. in this experiment, we maintained 6 pigs in closed economy environments for three 120-hr choice periods in whi ...200314965785
adenoviral catheter-mediated intramyocardial gene transfer using the mature form of vascular endothelial growth factor-d induces transmural angiogenesis in porcine heart.it is unclear what is the most efficient vector and growth factor for induction of therapeutic vascular growth in the heart. furthermore, the histological nature of angiogenesis and potential side effects caused by different vascular endothelial growth factors (vegfs) in myocardium have not been documented.200414967735
laparoscopic partial nephrectomy using holmium laser in a porcine model.to evaluate the utility of the holmium laser for partial nephrectomy in a porcine model.200414974664
isolation and morphology of single purkinje cells from the porcine heart.purkinje cells were isolated from both ventricles of young adult domestic pigs and examined by transmitted light or laser scanning confocal microscopy. purkinje cells in free running purkinje fibres were organised in multicellular strands where individual cells were tightly connected end-to-end and closely side-to-side. after isolation, single cells gradually lost the elongated appearance and became more rounded, but the cell membrane remained smooth and undamaged. the contractile material was n ...200314986884
biological transmission of vesicular stomatitis virus (new jersey serotype) by simulium vittatum (diptera: simuliidae) to domestic swine (sus scrofa).the role of hematophagous arthropods in vesicular stomatitis virus (new jersey serotype; vsv-nj) transmission during epizootics has remained unclear for decades in part because it has never been shown that clinical or subclinical disease in a livestock host results from the bite of an infected insect. in this study, we investigated the ability of vsv-nj-infected black flies (simulium vittatum zetterstedt) to transmit the virus to domestic swine, sus scrofa l. experimental evidence presented here ...200414989350
3d pulmonary perfusion mri and mr angiography of pulmonary embolism in pigs after a single injection of a blood pool mr contrast agent.the purpose of this study was to assess the feasibility of contrast-enhanced 3d perfusion mri and mr angiography (mra) of pulmonary embolism (pe) in pigs using a single injection of the blood pool contrast gadomer. pe was induced in five domestic pigs by injection of autologous blood thrombi. contrast-enhanced first-pass 3d perfusion mri (te/tr/fa: 1.0 ms/2.2 ms/40 degrees; voxel size: 1.3 x 2.5 x 4.0 mm3; ta: 1.8 s per data set) and high-resolution 3d mra (te/tr/fa: 1.4 ms/3.4 ms/40 degrees; vo ...200414997336
changes of lymphatic flow in case of pancreatic duct obstruction in the pig--as a model of pancreatic cancer.to investigate the lymph network change in pancreatic duct obstruction in pigs as a model of pancreatic cancer invading pancreatic duct, six domestic pigs weighing 17-40 kg underwent surgery as protocol. two of them were controls, and the others underwent ligation of the pancreatic duct as a model of ductal obstruction. a ch 40 and lipiodol mixture was injected in their pancreas at 7 or 21 days after first operation. radiographic examination had been also performed. five or 14 days later, they w ...200415000259
routine vascular control using the hem-o-lok clip in laparoscopic nephrectomy: animal study and clinical application.during laparoscopic nephrectomy (lpn), a stapling device is often used for vascular control, especially of the renal vein. herein, we report our experience using a polymer clip (hem-o-lok) for routine control of the vessels during lpn in the animal and clinical setting.200415006060
needle-based ablation of renal parenchyma using microwave, cryoablation, impedance- and temperature-based monopolar and bipolar radiofrequency, and liquid and gel chemoablation: laboratory studies and review of the literature.small renal tumors are often serendipitously detected during the screening of patients for renal or other disease entities. rather than perform a radical or partial nephrectomy for these diminutive lesions, several centers have begun to explore a variety of ablative energy sources that could be applied directly via a percutaneously placed needle-like probe. to evaluate the utility of such treatment for small renal tumors/masses, we compared the feasibility, regularity (consistency in size and sh ...200415006061
effect of food consistency on temporomandibular joint morphology: an experimental study in pigs.to investigate whether there are any correlations between increased masticatory loading, degree of tooth wear, and the size, form, and macroscopic surface of the temporomandibular joints (tmjs).200415022535
downregulation of n-cadherin in the neointima stimulates migration of smooth muscle cells by rhoa deactivation.the aim of the study was to analyze whether cadherin- and rho-family gtpases-mediated dynamic rearrangement of cell-cell adhesion play an important role during human arterial smooth muscle cell (hasmc) migration.200415023568
respiratory changes during prolonged increased intra-abdominal pressure in pigs.increased intra-abdominal pressure (iap) elevates thoracic pressure and airway pressures and reduces lung compliance in humans and laboratory animals. we studied respiratory alterations and arterial blood gas changes in pigs with iap maintained at 20 mmhg or 30 mmhg for 3 h.200415025609
prevalence of porcine endogenous retrovirus in domestic pigs in japan and its potential infection in dogs xenotransplanted with porcine pancreatic islet cells.the prevalence of porcine endogenous retrovirus (perv) proviral dna among various pig breeds raised in japan was investigated by polymerase chain reaction (pcr). moreover, potential infection of perv was investigated by pcr and reverse transcriptase-polymerase chain reaction (rt-pcr) in experimentally induced diabetic dogs (n=5) implanted with the diffusion chamber type bio-artificial endocrine pancreas (bio-aep) containing porcine pancreatic endocrine (pe) cells. no immunosuppressant was used a ...200415031539
[histological investigation of the micromorphological effects of the application of a laser needle--results of an animal experiment].in an experimental animal study (sus scrofa domesticus) we investigated the effects of the new technique of laser needle stimulation (wavelength: 685 nm; energy density: 4.6 kj/cm2 per point; application duration: 20 min). the results revealed changes in microcirculatory parameters of the skin resulting in an increase in blood flow. however, the quality and intensity of the laser light did not induce micromorphological alterations in the skin.200415032490
novel herpesviruses of suidae: indicators for a second genogroup of artiodactyl gammaherpesviruses.five novel herpesviruses were identified in suid species from africa (common warthog, phacochoerus africanus) and south-east asia (bearded pig, sus barbatus; babirusa, babyrousa babyrussa) by detection and analysis of their dna polymerase genes. three of the novel species, p. africanus cytomegalovirus 1, p. africanus lymphotropic herpesvirus 1 (pafrlhv-1) and s. barbatus lymphotropic herpesvirus 1 (sbarlhv-1), were closely related to known beta- (porcine cytomegalovirus) and gammaherpesviruses [ ...200415039528
prevalence of antibodies to hepatitis e virus among apparently healthy humans and pigs in bali, indonesia: identification of a pig infected with a genotype 4 hepatitis e virus.in indonesia where hepatitis e virus (hev) is believed to be highly endemic, only three outbreaks of hev transmission have been documented to date in restricted areas (west kalimantan and east java). a total of 1,115 serum samples collected from apparently healthy individuals in bali, lombok, and surabaya in indonesia in 1996 where epidemic hev transmissions have never been reported, were tested for igg class antibodies to hev (anti-hev). in bali, anti-hev was detected in 20% (54/276) of the tes ...200415042646
linkage disequilibrium in the domesticated pig.this study investigated the extent of linkage disequilibrium (ld) in two genomic regions (on chromosomes 4 and 7) in five populations of domesticated pigs. ld was measured with d' and tested for significance with the fisher exact test. effects of genetic (linkage) distance, chromosome, population, and their interactions on d' were tested both through a linear model analysis of covariance and by a theoretical nonlinear model. the overall result was that (1) the distance explained most of the vari ...200415082558
new metallic mr stents for artifact-free coronary mr angiography: feasibility study in a swine model.the objective of this study was to investigate the potential for artifact-free coronary magnetic resonance angiography (cmra) in the presence of dedicated metallic mr stents in vitro and in a swine model.200415087718
epidemiological study on porcine circovirus type 2 (pcv2) infection in the european wild boar (sus scrofa).porcine circovirus type 2 (pcv2) is considered as the causative agent of postweaning multisystemic wasting syndrome (pmws) in domestic pigs, where the virus is ubiquitous as evidenced by serological surveys. we present the results of the first nationwide sero-survey on the presence of pcv2 antibodies in european wild boars, and report the first pmws case in a wild boar from spain. sera from 656 hunter harvested wild boars from 45 different geographical sites and 22 additional imported animals we ...200415099500
immunogenicity and t cell recognition in swine of foot-and-mouth disease virus polymerase 3d.immunization of domestic pigs with a vaccinia virus (vv) recombinant expressing foot-and-mouth disease virus (fmdv) 3d protein conferred partial protection against challenge with infectious virus. the severity reduction of the clinical symptoms developed by the challenged animals occurred in the absence of significant levels of anti-3d circulating antibodies. this observation suggested that the partial protection observed was mediated by the induction of a 3d-specific cellular immune response. t ...200415110524
surgical construction of an in vivo carotid siphon model to test neurovascular devices.we developed an animal model to evaluate vascular trauma induced by endovascular devices that are proposed for the treatment of cerebrovascular diseases.200415113480
acute myocardial infarction: contrast-enhanced multi-detector row ct in a porcine model.to assess the role of contrast material-enhanced retrospectively electrocardiographically (ecg) gated multi-detector row computed tomography (ct) in the detection of acute myocardial infarction in a porcine model of total coronary occlusion.200415118118
emerging encephalitogenic viruses: lyssaviruses and henipaviruses transmitted by frugivorous bats.three newly recognized encephalitogenic zoonotic viruses spread from fruit bats of the genus pteropus (order chiroptera, suborder megachiroptera) have been recognised over the past decade. these are: hendra virus, formerly named equine morbillivirus, which was responsible for an outbreak of disease in horses and humans in brisbane, australia, in 1994; australian bat lyssavirus, the cause of a severe acute encephalitis, in 1996; and nipah virus, the cause of a major outbreak of encephalitis and p ...200415119765
the effects of birth weight and postnatal growth patterns on fat depth and plasma leptin concentrations in juvenile and adult pigs.low birth weight is associated with altered adipose tissue deposition and regulation of leptin production. this study determined the effects of naturally occurring variations in birth weight in pigs on postnatal growth patterns, body fat depth and plasma leptin and other hormone concentrations. low (< 1.47 kg) and high (> 1.53 kg) birth weight piglets were studied at 3 months (juvenile; n= 47) and 12 months of age (young adult; n= 17). at each age, arterial and venous catheters were inserted und ...200415121800
delayed portal vein thrombosis after experimental radiofrequency ablation near the main portal vein.portal venous blood flow may protect adjacent tumour cells from thermal destruction with radiofrequency ablation (rfa). this study aimed to investigate the local effect of rfa on the main portal vein branch, and the completeness of cellular ablation in its vicinity, with or without a pringle manoeuvre using a porcine model.200415122617
a novel population of calretinin-positive neurons comprises reelin-positive cajal-retzius cells in the hippocampal formation of the adult domestic pig.calretinin-containing neurons in the hippocampal formation, including the subiculum, presubiculum, parasubiculum, and entorhinal cortex, were visualized with immunocytochemistry. calretinin immunoreactivity was present exclusively in non-principal cells. the largest immunoreactive cell population was found in the outer half of the molecular layer of the dentate gyrus and in the stratum lacunosum-moleculare of ammon's horn. a proportion of these cells were also immunoreactive for reelin, a cajal- ...200415132437
interaction between a perfluorocarbon emulsion and radiographic contrast media.this study evaluated specially designed perfluorocarbon (pfc) emulsions as blood substitutes in case of induced ischemia of the left heart ventricle in healthy farm pigs. two hundred ml of perfluorocarbon emulsion were infused while 200 ml of blood were simultaneously drawn. radiographic contrast media were given to aid placement of balloon catheters in the left coronary artery. histopathological analysis showed that right heart failure caused the deaths of both pigs. particles (up to>3 micro) o ...200415152157
splanchnic metabolism during gut ischemia and short-term endotoxin and hemorrhagic shock as evaluated by intravasal microdialysis.the splanchnic area is of considerable interest in different types of shock. to characterize the metabolic changes in the splanchnic region in response to different types of shock we used a model where shock-induced metabolic changes in the splanchnic region were studied by the use of intravasal microdialysis. 23 anesthetized domestic pigs were randomized into four groups: group i, serving as controls (n = 5); group ii, mesenteric ischemia for 180 followed by 120 min of reperfusion (n = 5); grou ...200415167688
ultrasound strain imaging of altered myocardial stiffness: stunned versus infarcted reperfused myocardium.in this study we evaluate the diastolic deformation of ischemic/reperfused myocardium and relate this deformation to tissue elastic properties.200415173032
load dependence of cardiac output in biventricular pacing: right ventricular pressure overload in pigs.the effect of biventricular pacing on stroke volume is believed to be dependent on right ventricular/left ventricular delay, but effects in individual patients are unpredictable. this variability may reflect relative right and left ventricular volume and/or pressure overloads. accordingly, we tested the hypothesis that the relation of cardiac output to right ventricular/left ventricular delay is load dependent in a pig model of pulmonary stenosis.200415173728
initial study of arthropod succession on pig carrion in a central european urban habitat.we conducted a carrion succession study within a restricted urban backyard in the city of vienna, austria (16 degrees 22'e, 48 degrees 12'n) from may to november 2001 to analyze sequence and composition of the local carrion visiting fauna. two medium sized clothed domestic pig carcasses (sus scrofa linnaeus), were used as surrogate human models. in total, 42 arthropod species from the families calliphoridae, sarcophagidae, sepsidae, piophilidae, muscidae, fanniidae, sphaeroceridae, phoridae, dro ...200415185958
in vitro coronary artery imaging with sixteen-slice helical computed tomography.to determine the benefits of submillimeter coronary computed tomography (ct) angiography in vitro.200415191099
detection and genetic characterization of porcine circovirus type 2 (pcv2) in pigs from croatia.porcine circovirus type 2 (pcv2) from the circoviridae family has recently been associated with two serious diseases of swine, post-weaning multisystemic wasting syndrome (pmws) and porcine dermatitis and nephropathy syndrome (pdns). during 2002, several outbreaks of clinical disease in pigs with weights ranging from 10 to 70 kg occurred on four farms in different locations in croatia. the signs were consistent with pmws and pdns. apart from progressive weight loss, pneumonia and/or diarrhoea, m ...200415196907
an isoenzyme survey of trypanosoma brucei s.l. from the central african subregion: population structure, taxonomic and epidemiological considerations.in order to improve our knowledge about the taxonomic status and the population structure of the causative agent of human african trypanosomiasis in the central african subregion, 169 newly isolated stocks, of which 16 came from pigs, and 5 reference stocks, were characterized by multilocus enzyme electrophoresis, for 17 genetic loci. we identified 22 different isoenzyme profiles or zymodemes, many of which showed limited differences between them. these zymodemes were equated to multilocus genot ...200415206467
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