Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| acetylcholine and substance p: action via distinct receptors on carp retinal ganglion cells. | substance p (sp), a neuropeptide, has been found in amacrine cells in a variety of vertebrate retinas. sp excited many of the ganglion cells we sampled in carp retina; many of these ganglion cells are directly excited by cholinergic agonists. it has been reported that sp modulates cholinergic synaptic transmission in some other neuronal systems by inhibiting or desensitizing cholinergic receptors. sp does not act in this way on the ganglion cell cholinergic receptors in isolated carp retina. pul ... | 1982 | 6176567 |
| the effect of primary and secondary immunization on the lymphoid tissues of the carp, cyprinus carpio l. | mirror carp immunized with human gamma globulin (hgg) in freund's complete adjuvant (fca) show a proliferative response involving cells whose cytoplasm stains deep red with methyl green-pyronin (pyroninophilic cells). this response occurs particularly in the haemopoietic parenchyma of the pronephros and mesonephros. it peaks at week 3, with the formation of clusters of pyroninophilic cells in the pronephros. immunization with aeromonas salmonicida elicited a less intense pyroninophilic response ... | 1982 | 6177828 |
| calcium alters the sensitivity of intact horizontal cells to dopamine antagonists. | horizontal cells of the carp retina possess dopamine receptors linked to adenylate cyclase. isolated, intact horizontal cells respond to micromolar concentrations of dopamine, whereas nanomolar concentrations of haloperidol, (+)-butaclamol, and flupenthixol block the dopamine response. preincubation in ringer's solution containing increased levels of ca2+ (5-110 mm) decreases the sensitivity of the cells to these antagonists by 1,000-10,000 times. dopamine sensitivity of the cells is not affecte ... | 1982 | 6179080 |
| behavior of rna and protein synthesis during the acclimatization of the carp. studies with isolated hepatocytes. | 1. carp hepatocytes were isolated by dissociating the liver tissue with collagenase. the procedure yields viable cells with highly preserved ultrastructural and metabolic features. the isolated cells were able to self-aggregated and form tissue. 2. rna and protein synthesis activity was significantly higher in the carp hepatocytes from summer acclimatized fish compared to the activity present in the cold adapted animals. 3. rna synthesis assayed in carp hepatocytes suspensions obtained from summ ... | 1982 | 6179711 |
| localization of immune complexes and heat-aggregated immunoglobulin in the carp cyprinus carpio l. | in carp cyprinus carpio l. preimmunized with a protein antigen, human gamma globulin (hgg), rapid localization of antigen occurred in the splenic ellipsoids within 1 day of a secondary immunization given 8 weeks later. furthermore, after injection of immune complexes or heat-aggregated hgg, antigen was trapped more quickly in the pronephros than when antigen was injected alone. these results support the conclusion that in fish, as in homoiotherms, this form of antigen trapping is antibody-depend ... | 1982 | 6180972 |
| [tolerance induction and production of anti-idiotypic antisera in carp]. | tolerance in carps can be induced by intracardial injection of 10-30 mg of ultracentrifuged human gamma globulin (hggu), with strong individual variation being observable. out of 21 carps pretreated with 10 mg hggu, 10 animals showed 14 days after i.p. test immunization with 1 mg hggu a log2 anti-hgg titre of 1.55 +/- 0.60 (control group - log2 7.33 +/- 1.47), while 11 animals exhibited a log2 titre of 7.45 +/- 2.75. low doses of hggu (0.01-1.0 mg) led to stimulation of immune response. if 1-2 m ... | 1982 | 6183853 |
| the cells involved in the immune response of fish: ii. pha-induced clonal proliferation of carp lymphocytes in soft agar culture. | lymphocytes from peripheral blood of carp proliferate in a clonal culture in soft agar, in the presence of phytohemagglutinin, generating several morphologically distinct types of colonies. cells from colonies developing on the surface of the agar (surface colonies) and cells from colonies developing within the agar (agar colonies) were studied. several differences were found between cells from the two types of colonies with respect to morphology, ultrastructure and the distribution of cytoplasm ... | 1982 | 6186537 |
| development and validation of a carp growth hormone radioimmunoassay. | purified growth hormone (gh), isolated from pituitary glands of the common carp (cyprinus carpio), was shown to have biochemical and immunological properties in common with other teleost gh preparations. intraperitoneal injections of the carp gh, at a dose of 1 microgram/g body wt, resulted in significant increases in body weight in goldfish. in addition, the carp gh was used to prepare an antiserum for the development of a radioimmunoassay (ria). the displacement curves for serum from goldfish ... | 1983 | 6193032 |
| effects of vasoactive intestinal peptide and other peptides on cyclic amp accumulation in intact pieces and isolated horizontal cells of the teleost retina. | the effects of vasoactive intestinal peptide (vip) and several other peptides have been examined on cyclic amp accumulation in intact pieces and isolated horizontal cells of the teleost (carp) retina. vip was the most effective peptide examined, inducing a dose-related response, and an approximately fivefold increase in cyclic amp production when used at a concentration of 10 microm. porcine histidine isoleucine-containing peptide and secretin, peptides structurally related to vip, also stimulat ... | 1983 | 6194261 |
| ontogeny of the immune system in carp (cyprinus carpio l.). the appearance of antigenic determinants on lymphoid cells detected by mouse anti-carp thymocyte monoclonal antibodies. | mouse anti-carp thymocyte monoclonal antibodies were used to investigate the appearance of lymphoid cell determinants in young carp. determinants detected by clone wct 4 were present on thymocytes as soon as the thymus was visible histologically, day 4 after fertilization (a.f.) at 21 degrees c, but wct 4-positive lymphocytes were not present in the pronephros until day 7 a.f.. in contrast, determinants detected by monoclonal antibodies from clones wct 8 and wct 10 appeared on thymocytes from da ... | 1983 | 6196239 |
| sulfomucins and sialomucins in the mucus cells of the alimentary canal in an indian freshwater major carp, labeo rohita (hamilton): a fluorescence microscopic study. | the localization of sulfomucins and sialomucins present in the various types of mucus cells--secretory (smc) and mature non-secretory mucus cells (mmc) of the different regions of the alimentary canal in labeo rohita (ham.) has been studied by fluorescence microscopical techniques (viz., ferric alum coriphosphine--fac, coriphosphine thiazol yellow--cty and dische reaction--dr). high degree of fluorescence activity confirms the presence of relatively high amount of sulfomucins in the mucus cells ... | 1983 | 6199266 |
| monoclonal antibodies against spermatozoa of the common carp (cyprinus carpio l.). i. a study germ cell antigens in adult males and females. | eleven monoclonal antibodies that recognize membrane determinants on spermatozoa of the carp cyprinus carpio l. have been produced. indirect immunofluorescence revealed that these determinants are uniformly distributed on the surface of head and midpiece. most of them are also present on the outer membrane of precursor sperm cells. although none of the monoclonal antibodies reacted with carp somatic tissue, five monoclonal antibodies were positive for surface membrane determinants of oogonia and ... | 1984 | 6201282 |
| radioimmunochemical studies on 7.8s and 5.7s duck immunoglobulins in comparison with fab and fc fragments of chicken igy. | in ducks two forms of low molecular weight ig's +), 7.8s and 5.7s, have been described. to compare their antigenicity with fab and fc fragments of chicken igy a double-antibody ria technique was used in which the binding of 125i-chicken igy or 125i-chicken igy (fc) to rabbit and carp anti-chicken igy (fab) and anti-chicken igy (fc) antibodies, respectively, was inhibited by duck 7.8s and 5.7s ig's. chicken igy and duck 7.8s ig ( igy -like protein) are highly cross-reactive with respect to their ... | 1984 | 6202568 |
| pharmacological properties and localization of dopamine-sensitive adenylate cyclase in the carp retina. | there is a specific dopamine (da)-sensitive adenylate cyclase (ec 4.6.1.1) in the carp retina that can be stimulated by da, da agonists, and potassium. experiments with the presynaptic blocker cobalt and the neurotoxin 6-hydroxydopamine strongly indicate that this adenylate cyclase is located postsynaptically. by separating retinal neurons with velocity sedimentation at unit gravity it was determined that horizontal cells possessed a da-sensitive adenylate cyclase. the sensitivity of these cells ... | 1984 | 6205901 |
| an extensive projection of fish dorsolateral tegmental cells to the optic tectum revealed by intra-axonal dye marking. | the cells of the dorsolateral tegmental (dlt) nucleus in the crucian carp (carassius carassius) were physiologically identified and marked with lucifer dye. all the identified dlt cells receive both visual and rhombencephalic inputs. these cells project their axons into the contralateral tectum via the tectal commissure as well as into the ipsilateral tectum. the most striking characteristic of the dlt cells was the wide distribution of their axonal branching in the ipsilateral tectum. | 1984 | 6207471 |
| specific activity of carp antisera against beta 2-microglobulin (beta 2m) and evidence for a beta 2m homologue in carp (cyprinus carpio). | antisera against rabbit and human beta 2-microglobulin were produced in carp (cyprinus carpio). these antisera were found to be specific for beta 2m, and detected beta 2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. in addition, these antisera were also inhibited by an extract from oyster. the differences between the reactivity of these two antisera and the ability of the anti-rabbit beta 2m to distinguish between ... | 1984 | 6209173 |
| immunoreactive opsin in the pineal organ of reptiles and birds. | the presence of opsin was investigated with light microscopic immunocytochemistry in pinealocytes of reptiles and birds (emys orbicularis, pseudemys scripta elegans, lacerta agilis et viridis, gallus domesticus, columba livia, melopsittacus undulatus, serinus canaria, taeniopyga punctate). the outer segments of pinealocytes selectively bound antiopsin antibody as revealed by indirect immunocytochemical techniques, indicating the occurrence of a rhodopsin-like photopigment in these structures. th ... | 1982 | 6213109 |
| ouabain-insensitive mechanism of cell volume maintenance in the carp kidney. | 1) an ouabain-insensitive mechanism of cell volume maintenance, similar to that operating in the kidney tissue of mammals and in epithelial cells of the frog urinary bladder, has been demonstrated in the kidney tissue of carp (cyprinus carpio)--there is no significant swelling even after 4-hour incubation with 10(-4) mol/l-1 ouabain. 2) the mechanism is not affected by 2-hour incubation with sodium in the saline replaced with tris or choline and, unlike in rabbit kidney cortex, cannot be inhibit ... | 1982 | 6214809 |
| biochemical polymorphisms in south african freshwater fish. isoenzyme patterns in fish of the families cyprinidae and salmonidae. | isoenzyme patterns were studied in local populations of the carp (cyprinus carpio) and the bream (sarotherodon mossambicus) of the cyprinid family, and in the trout (salmo giardneri) of the family salmonidae. homogenates of heart muscle extracts were used in the identification of pgi, nadp-idh, pgm and 6-pgd. polymorphisms found and gene frequencies obtained are discussed separately for each enzyme and species or population. in the bream, variation was found only at the pgi locus. in the carp on ... | 1982 | 6214975 |
| [effects of a gonadotropic hormone of teleost fish on the adenylate cyclase of rat ovary and on its stimulation by ovine gonadotropins]. | carp gonadotropin (cgth) stimulated adenylate cyclase from 20-24 day old (but not 16-18 day old) rat ovaries; its subunits were inactive. carp gth (but not its subunits) increased the apparent kd for ofsh but not for olh. these results suggest that cgth mainly interacts with fsh sensitive adenylate cyclase-receptor systems, with an efficiency about 6 x 10(3) times lower than ofsh itself. | 1982 | 6220787 |
| effect of synthetic lys-trp on adenosine triphosphatase activity of carp and rabbit myosin bs. | 1. with carp myosin b atpase, the acceleration effect was found at peptide concentration ranging between 1 nm and 1 mm with a maximum at 10 nm (30% acceleration) at the low myosin b concentration (0.2 mg/ml). at the high myosin b concentration (0.8 mg/ml) a maximum was observed at 1-10 mm (10% acceleration). 2. with rabbit myosin b atpase, the acceleration (10%) was found only at a microm of lys-trp in the case of low myosin b concentration (0.2 mg/ml). under the high concentration of rabbit myo ... | 1983 | 6221861 |
| muscle fibre composition of the eye muscles of the carp (cyprinus carpio l.) defined on the base of histochemical observations. | a histochemical study has been carried out on the eye muscles of the carp. on the base of the atp-ase and sdh activity and with regard to the localization and diameter of muscle fibres six types of muscle fibres situated in the defined zones can be distinguished. the type 1 and 2 fibres display a moderately high atp-ase activity at ph 9.4 and rather low activity after alkaline and acid preincubation. type 1 shows a high sdh activity in contrast to type 2 with a low sdh activity. the other four t ... | 1983 | 6224725 |
| morphological and biochemical studies on liver, kidney and gill of fishes affected by pesticides. | effect of a herbicide, paraquat (1,1'-dimethyl-4,4'-bipyridilium-dichloride), the fungicide copper sulphate, and zinc chloride was studied on the histological structure of liver, kidney and gill of three fish species with different feeding habits, viz.: a herbivorous, silver carp (hypophthalmichthys molitrix); an omnivorous, common carp (cyprinus carpio l.) and a carnivorous, sheatfish (silurus glanis l.). the organs were studied electron microscopically after fixation according to karnovsky. th ... | 1983 | 6230560 |
| aporphine enantiomers. interactions with d-1 and d-2 dopamine receptors. | the r(-) and the s(+) enantiomers of apomorphine (apo) and n-n-propyl norapomorphine (npa) interact with both the d-1 and the d-2 dopamine receptors. r(-)-apo, as well as r(-)- and s(+)-npa, stimulates the d-1 dopamine receptor in carp retina; s(+)-apo blocks this dopamine receptor. similarly, r(-)-apo, as well as r(-)- and s(+)-npa, stimulates the d-2 dopamine receptor in the intermediate lobe of the rat pituitary gland; s(+)-apo blocks the intermediate lobe d-2 receptor. the interactions betwe ... | 1984 | 6231468 |
| studies on the biology and pathology of the common carp parasite myxobolus basilamellaris lom et molnár, 1983 (myxozoa: myxosporea). | 1983 | 6234773 | |
| phylogeny of interleukins: growth factors produced by leucocytes of the cyprinid fish, cyprinus carpio l. | supernatants of phytohaemagglutinin (pha)-activated pronephric leucocytes from carp (cyprinus carpio l.) contain a lymphocyte growth factor which can induce a proliferative response of purified lymphoblasts but not freshly isolated leucocytes. the growth-promoting activity can be reduced by absorbing the supernatant with mitogen-activated blasts. in addition, increased incorporation of tritiated thymidine into pha-activated blast cells is also induced by supernatants from two-way mixed leucocyte ... | 1984 | 6235177 |
| the mixed leukocyte reaction (mlr) in carp: bidirectional and undirectional mlr responses. | two-way and one-way mixed leukocyte reaction (mlr) was demonstrated in peripheral blood leukocytes (pbl) of carp. primary two-way mlr in randomly selected donor pairs were highly variable. weak primary responses could be strongly augmented by mutual in vivo priming of the reacting donors. one-way mlr was performed using irradiated (16,000 r) allogeneic pbl as stimulators. reciprocal responses of randomly paired donors were usually unequal, suggesting the usefulness of this method for genetic ana ... | 1984 | 6238857 |
| effects of a herbicide on the peroxide metabolism enzymes and lipid peroxidation in carp fish (hypophthalmichthys molitrix). | a study was made of the peroxide metabolism enzymes in carp fish, and of the effects on these of three different concentrations of the herbicide paraquat. mainly the changes in the superoxide dismutase, catalase and lipid peroxidation resembled the changes observed in previous intoxication studies on mammals. | 1984 | 6239479 |
| [a further confirmation of the high idiotypic specificity of carp anti-human myeloma protein sera]. | previous investigations about the specificity of carp anti-idiotypic sera could be confirmed by comparison with corresponding antisera of guinea pigs. 3 carp antihuman myeloma-protein sera rendered as idiotypic specific after an appropriate absorption were tested in the passive hemagglutination inhibition test. they showed no difference to the parallel tested guinea pig antisera. it can be assumed that between fish antibodies and antibodies of mammals no functional difference exists in respect o ... | 1984 | 6240937 |
| [non-phosphorylative transglycosylation in extracts from white and red muscles of carp (cyprinus carpio l.)]. | the enzymatic, nonphosphorylative reaction of transglycosylation in extracts from white and red muscles of the carp was studied. amylomaltase (ec 2.4.1.25) activity was assayed using p-nitrophenyl-n-maltoside as substrate and the amount of p-nitrophenyl-n-maltotrioside was assumed as a measure of the enzyme activity. the ph and temperature optimum of the reaction was examined. in both types of muscles significant differences in transglycosylative activity and its decrease immediately after prolo ... | 1984 | 6242767 |
| [oxidative processes in carp liver mitochondria during adaptation to changes of co2 concentration in water]. | two-years-old carps (cyprinus carpio l.) were kept for 1,3 and 7 days in the medium with different concentrations of co2. in the fish liver mitochondria the rate of co2 uptake (state 4p) and phosphorylation (state 3) as well as the value of the respiratory control and the activity of cytochrome oxidase and succinate dehydrogenase undergo significant changes. in fishes which were in the medium with 0.4 mm co2 for a day the cytochrome oxidase activity in the mitochondria increases sharply, but tha ... | 2015 | 6247794 |
| [peculiarities of phosphoric compound metabolism in liver mitochondria of carp adapted to higher concentrations of co2 in water]. | the content of adenine nucleotides, atpase activity, the amount of total and inorganic phosphorus in the carp liver mitochondria were studied as affected by co2 high concentrations. it is shown that during adaptation to the co2 higher level in the medium the amount of atp in fishes undergoes the most significant changes. the organism response to the effect of carbon dioxide depends on its concentration in the medium and time of its action. when fishes were for 24h under conditions of the 0.4mm c ... | 2015 | 6247795 |
| [glycogen content and activities of phosphorylase and glucose-6-phosphatase in the fast and slow muscles of representatives of different classes of vertebrates]. | studies have been made on glycogen content as well as on the activity of phosphorylase and glucose-6-phosphatase in fast and slow muscles from representatives of 6 classes of vertebrates (lampetra fluviatilis, cyprinus carpio, rana temporaria, rana ridibunda, emys orbicularis, hen, rat). glycogen level and glucose-6-phosphatase activity are either higher in slow muscles, or practically identical in both types of muscles (glucose-6-phosphatase is absent from the fast muscles of hens and rats). on ... | 1980 | 6247864 |
| effect of alkyl chain on the uptake, distribution, and excretion of 35s-labeled alkyl sulfates in carp. | 1980 | 6248315 | |
| demonstration of specific receptors of the rough endoplasmic membrane for the signal sequence of carp preproinsulin. | 1. evidence is given for the cotranslational processing in a wheat germ cell-free system of carp preproinsulin to proinsulin by dog pancreatic rough endoplasmic membranes stripped of ribosomes by edta. concomitantly with processing there is a translocation of the protein across the membrane as shown by the accessibility to proteases. conversion of preproinsulin to proinsulin occurs post-translationally only in the presence of detergent. 2. stripped rough endoplasmic membranes bind a significant ... | 1980 | 6249584 |
| circular dichroism and spin-label studies of carp hemoglobin. | 1980 | 6253650 | |
| temperature adaptation of biological membranes. compensation of the molar activity of cytochrome c oxidase in the mitochondrial energy-transducing membrane during thermal acclimation of the carp (cyprinus carpio l.). | the acclimation temperature of carp does not affect the amount of cytochrome c oxidase per mg mitochondrial protein as revealed from the reduced-minus-oxidized difference spectra of red muscle mitochondria from cold- and warm-acclimated carp. there are no differences between cold- and warm-acclimated fish in the substrate binding properties of the enzyme as judged from the km values for cytochrome c at 30 degrees c (3.34 +/- 0.ee microm, acclimation temperature 10 degrees c and 3.55 +/- 0.31 mic ... | 1981 | 6260175 |
| [dendritic potentials in granular cells of carp olfactory bulbs]. | fast prepotentials in the granular interneurons of carp olfactory bulb were recorded by means of intracellular microelectrodes. data are presented indicating that fast prepotentials are generated in the electrogenic membrane of granular cell dendrites. it is supposed that dendritic spikes play an important role in the inhibitory synaptic influences of granular cells upon secondary olfactory neurons. | 1981 | 6261168 |
| [subunits of lutropins: an intrinsic gonadotropic activity of alpha and the role of beta in the determination of the zoological specificity of action]. | pieces of eel ovary were incubated in presence of various gonadotropic hormones or their subunits (alpha, beta) and cyclic adenosine monophosphate tissue concentration was measured. carp gonadotropin (c-gth) and a hybrid (bovine lutropin alpha-c-gth beta) exhibited a very similar, intense, dose-dependent activity in the range 0.002-1 microgram/ml. mammalian lutropins (lh) and their subunits became active in the range 1-100 microgram/ml: a strong stimulation (#30x) was attained with bovine lh alp ... | 1981 | 6263510 |
| indoleamine-accumulating cells and dopaminergic cells are distributed similarly in carp retina. | carp (cyprinus carpio) retinas were processed for simultaneous visualization of dopaminergic (da) and indoleamine-accumulating (id) cells in flat-mounts under fluorescence microscope. a mixture of 5 micrograms noradrenaline (na) and 5 micrograms 5,6-dihydroxytryptamine (5,6-dht) was injected intravitreally into the eyes 2 h before enucleation. the spatial distribution and density were compared between na-loaded da-cells (greenish fluorescence) and 5,6-dht-loaded id-cells (yellowish). the two cla ... | 1981 | 6269027 |
| interaction of the putative dopamine autoreceptor agonists, 3-ppp and tl-99, with the dopamine-sensitive adenylate cyclase of carp retina. | the putative dopamine autoreceptor agonists, 3-ppp and tl-99 were examined for their ability to stimulate postsynaptic dopamine receptors associated with adenylate cyclase (d1-receptors) in the carp retina. in intact pieces of retina, 3-ppp had no significant effect on cyclic amp production at concentrations up to 100 microm, whereas the aminotetralin, tl-99, caused a concentration-dependent increase in cyclic amp levels with an approximate ec50 of 3.6 microm. dopamine and adtn had ec50 values o ... | 1982 | 6277674 |
| analyses of bipolar cell responses elicited by polarization of horizontal cells. | simultaneous intracellular recordings were made from a bipolar cell and a horizontal cell in the carp retina. the properties of the bipolar cell were studied while injecting current into the horizontal cell. hyperpolarization of horizontal cells, irrespective of their type, elicited a hyperpolarizing response in on-center bipolar cells and a depolarizing response in off-center bipolar cells. analyses of the ionic mechanisms of bipolar cell responses revealed that depolarization of horizontal cel ... | 1982 | 6278053 |
| isolated horizontal cells from carp retina demonstrate dopamine-dependent accumulation of cyclic amp. | horizontal cells of the carp retina were separated from other retinal cell types by using enzymatic dissociation and velocity sedimentation at unit gravity. fractions containing horizontal cells were tested for their ability to accumulate cyclic amp in the presence of various putative neurotransmitters. micromolar concentrations of dopamine, when added in the presence of 3-isobutyl-1-methylxanthine, stimulated cyclic amp accumulation in these isolated cells. the dopamine-dependent accumulation o ... | 1981 | 6278491 |
| carp horizontal cells in culture respond selectively to l-glutamate and its agonists. | horizontal cells were enzymatically isolated from the carp retina and maintained in culture for 2-7 days. cultured horizontal cells typically had resting membrane potentials of -50 to -70 mv and input resistances of 100-150 m omega. the cells were treated with a number of neurotransmitter agents and their analogues. significant responses were evoked only by 3,4-dihydroxyphenylalanine (dopamine), l-glutamate, and certain glutamate analogues. the responses to dopamine were inconsistent; most often ... | 1982 | 6278502 |
| histological and enzyme histochemical studies on the nephrons of the freshwater fishes, cyprinus carpio and carassius auratus. | the nephrons of carp (cyprinus carpio) and goldfish (carassius auratus) were examined histologically and also histochemically for enzymes. in both species the distal and collecting tubules have much wider lumens than do the other renal tubules; thus urine probably flows more slowly in these larger tubules. enzyme histochemistry shows that epithelium of the neck and proximal and intermediate tubules respires anaerobically, whereas that of the distal and collecting tubules respires aerobically. th ... | 1982 | 6286982 |
| inhibition of postsynaptic dopamine-sensitive adenylate cyclase in carp retina by the putative dopamine autoreceptor agonist, 3-ppp. | 1982 | 6290231 | |
| [metabolic aspects of temperature adaptation by fish]. | during 8-hour acclimation to changes in the water temperature by 10 degrees within the range of 20-30 degrees c the metabolic compensation of the temperature effects in the carp liver mitochondria is manifested at the level of thermogenesis, activity of succinate dehydrogenase and rate of oxidative phosphorylation. no temperature compensation is found for the activity of cytochromoxidase, atpase, rate of nonphosphorylating oxidation, content of atp, phosphorus and calcium in the mentioned organe ... | 2015 | 6291212 |
| electron microscopy of aminergic retinal neurons. | the synaptic connections of the dopaminergic retinal neurons of cynomolgus monkeys and rabbits were studied in the electron microscope. in the cynomolgus monkeys, a labelling method with 5,6-dihydroxytryptamine was used, whereas an autoradiographic technique with a monoamine oxidase inhibitor and (3h)-dopamine was used in the rabbits. the labelling method with 5,6-dihydroxytryptamine altered the normal ultrastructure of the dopaminergic neurons, but their synaptic structures were still easily re ... | 1982 | 6291309 |
| fine structure and function of isolated gonadotropic cells as revealed from pituitaries of immature rainbow trout, salmo gairdneri, by means of a new enzymatic dispersion technique. | a procedure has been developed for dissociating pituitary glands of juvenile rainbow trout, salmo gairdneri, producing a preparation of single dispersed pituitary cells in which morphological and functional integrity is preserved. the pituitaries are dispersed by sequential treatment with 0.1% collagenase, 0.04% ethylene-diamine-tetra-acetic acid (edta) and 0.125% dispase. the cell yield is 0.3--0.35 x 10(6) cells per pituitary with a cell viability percentage of 95 +/- 1% and single cell percen ... | 1982 | 6291764 |
| the mode of action of carp gonadotropin on the stimulation of androgen production by carp testis in vitro. | 1982 | 6292039 | |
| effects of intraocular injections of 6-hydroxydopamine on dopamine-dependent cyclic amp accumulation in intact pieces of carp retina. | dopamine-stimulated cyclic amp accumulation was measured in intact pieces of carp retina following intraocular injections of the dopaminergic neurotoxin 6-hydroxydopamine (6-ohda). this treatment is known to induce a selective destruction of dopaminergic nerve terminals, and in these experiments caused a 94% reduction in retinal dopamine content. however, dopamine-dependent cyclic amp accumulation was essentially unaltered in retinas exposed to 6-ohda, indicating that the dopamine receptors link ... | 1982 | 6295556 |
| [interaction of spin-labelled phosphatidyl choline with ca-dependent atpase from sarcoplasmic reticulum]. | the interaction between 3-acyl-2-(6-doxylpalmitoyl) phosphatidyl choline used as a hydrophobic spin probe and ca2+-dependent atpase from sarcoplasmic reticulum membranes of rabbit and carp white skeletal muscles was studied. the spin label incorporation into atpase preparations was performed at initial steps of atpase isolation by incubating reticulum membranes with the spin probe in the presence of cholic acid. a comparison of the molecular mobility of the probe incorporated into atpase prepara ... | 1982 | 6297625 |
| induction of melanization in primary cultures of dermal cells from xanthic goldfish scale by adrenocorticotropic hormone and goldfish or red carp serum. | 1983 | 6301695 | |
| development of a heterologous radioimmunoassay for eel (anguilla anguilla) gonadotropin. | a heterologous radioimmunoassay (ria) for eel gonadotropin (gth) was developed, using an antiserum to the beta subunit of carp gth (cgthbeta). partially purified eel gth preparations or crude eel pituitary extracts produced partial cross-reactions: the inhibition curves reached a plateau before complete inhibition of the binding of the tracer and the slopes were lower than those for cgth. however, the parallelism between the inhibition curves produced by all the eel samples allowed estimation of ... | 1983 | 6301935 |
| carp preproinsulin cdna sequence and evolution of insulin genes. | the nucleic acid sequence of the preproinsulin cdna of carp (cyprinus carpio), cloned in the psti-site of pbr322 (1), has been determined. the sequenced insert of 439 bp includes the complete coding information for carp preproinsulin (108 amino acids), 10 nucleotides of the 5'-and 105 nucleotides of the 3'-nontranslated regions. the nucleotide sequence confirms the previously established amino acid sequence of carp insulin (2) and determines those of the signal (21 aa 1) and c-peptide (35 aa 1). ... | 1983 | 6306593 |
| vasoactive intestinal peptide alters membrane potential and cyclic nucleotide levels in retinal horizontal cells. | vasoactive intestinal peptide stimulated the synthesis of adenosine 3',5'-monophosphate in fractions of isolated carp horizontal cells. when applied extracellularly to isolated and cultured horizontal cells, the peptide also induced a slow depolarization (30 to 40 millivolts) accompanied by a decrease in membrane resistance. however, analogs of adenosine 3',5'-monophosphate applied extracellularly or intracellularly, and forscolin applied extracellularly, had no effect on the membrane potential ... | 1983 | 6308770 |
| a gaba antagonist, bicuculline, exerts its uncoupling action on external horizontal cells through dopamine cells in carp retina. | external horizontal cells in isolated retinas of the carp (cyprinus carpio) were intracellularly recorded and marked with the fluorescent dye lucifer yellow (ly). these cells are electrically coupled via gap junctions, so that the injected dye normally diffused to neighboring cells. a gaba antagonist, bicuculline (bcc, 20 microm), applied to the vitreous fluid beneath the isolated retina, altered the spatial properties of light-induced responses, by increasing the amplitude of responses to centr ... | 1983 | 6310453 |
| an intracellular study of the optic tectum of the carp in vitro. | intracellular recordings were performed on the optic tectum of the carp in vitro. thick brain sections were prepared and the optic tract was electrically stimulated. epsps of 72 cells in stratum fibrosum et griseum superficiale, stratum griseum centrale and stratum album centrale were examine. monosynaptic epsps were recorded from 19 cells in stratum fibrosum et griseum centrale and stratum griseum centrale, which was consistent with anatomical findings. | 1983 | 6310457 |
| isolation, analysis of structure, synthesis, and biological actions of urotensin i neuropeptides. | the 41-residue neuropeptide urotensin i (ui), from the urophyses of two teleost fish species (cyprinus carpio and catostomus commersoni), was isolated and purified, and its amino acid sequence was determined and confirmed by synthesis of a fully active peptide. the ui peptide was found to be a close structural and biological homologue of the ovine hypothalamic corticotropin-releasing factor (crf) and the frog skin peptide sauvagine; ui is, therefore, a phylogenetic prototype of this group of pep ... | 1983 | 6313156 |
| poly(adp-ribose) synthetase associated to cytoplasmic structures of meiotic cells. | an active poly(adp-ribose) synthetase has been found to be associated to mitochondria of frog oocytes and of testis from various species. the enzyme was also found in association with the microsomal-ribosomes fraction of the testis. both enzymes are strongly inhibited by nicotinamide, thymidine, and theophylline, and they synthesize oligomers of adp-ribose of an average chain length of 2-6 residues. the apparent km for nad+ of the mitochondrial enzyme is 22 micro m which contrasts with the corre ... | 1983 | 6317634 |
| stimulation in vitro of estradiol secretion by the ovary of a cichlid fish, sarotherodon aureus. | the objective of the present study was to investigate the nature of the gonadotrophic stimulation of estradiol (e2) secretion by the ovary of a teleost fish in vitro. spontaneous output of e2 decreased to a low baseline after 3 hr of superfusion and increased to a maximal level 2-3 hr after the introduction of homologous pituitary extract (t-pe) or human chorionic gonadotrophin into the superfusion system. addition of the phosphodiesterase inhibitor, isobutyl methylxanthine (ibmx, 0.5 mm), into ... | 1984 | 6321293 |
| comparison of the primary sequence of spring viremia of carp virus m protein with that of vesicular stomatitis virus. | the nucleotide sequence of spring viremia of carp virus mrna coding for the matrix protein (m) has been determined from a complete dna copy cloned in the escherichia coli plasmid pbr322. the clone is 710 nucleotides long and, excluding the 3' homopolymeric end (representing the mrna polyadenylic acid sequence), encodes a protein of some 223 amino acids (25,600 da). the protein has more basic than acidic amino acids and no significant amino- or carboxy-terminal hydrophobic domains. the predicted ... | 1984 | 6324471 |
| synthetic competitive antagonists of corticotropin-releasing factor: effect on acth secretion in the rat. | polypeptide analogs of the known members of the corticotropin-releasing factor (crf) family were synthesized and tested in vitro and in vivo for enhanced potency or competitive antagonism. predictive methods and physicochemical measurements had suggested an internal secondary alpha-helical conformation spanning about 25 residues for at least three members of the crf family. maximization of alpha-helix-forming potential by amino acid substitutions from the native known sequences (rat/human and ov ... | 1984 | 6326264 |
| light-dependent change of cone-horizontal cell interactions in carp retina. | carp h2 horizontal cells were investigated at various stages of dark and light adaptation. their state of adaptation was verified by evaluating in computer reconstructed serial sections the number of small, fingerlike protrusions originating from h1 processes. these so-called spinules are absent in the dark-adapted state and are reformed during light adaptation. intracellular recordings showed that long-wave-induced depolarization thought to be mediated by sign-inverted feedback from h1 horizont ... | 1984 | 6326947 |
| [test systems for biomonitoring based on membrane-bound enzyme complexes. iv. assessment of genotoxic effects in an ames test system with metabolic activation by the microsomal mono-oxygenases from fish livers]. | the study of mutagenic effect of 2-aminoantracene and benz(alpha)pyrene on salmonella triphimurium ta 100 in the ames test-system in the presence of postmitochondrial fractions s-9 from carp liver with 3-methylcholantrene induced by microsomal oxidation system has been carried out. the metabolic activity and cytochrome p450 contence in carp liver microsomes have been shown to concede considerably those in rats liver. but these characteristics are sufficient for the use of fraction s-9 from carp ... | 1984 | 6329328 |
| a high-resolution proton nuclear-magnetic-resonance investigation of carp hemoglobin. conformational differences between carp and human normal adult hemoglobins in solution. | the high-resolution proton nuclear magnetic resonance spectra of carp hemoglobin have been compared to those of human normal adult hemoglobin. carp deoxy and carbonmonoxy hemoglobins in the deoxy-type quaternary state exhibit two downfield exchangeable proton resonances as compared to four seen in human normal adult deoxyhemoglobin. this suggests that two of the hydrogen bonds present in human normal adult deoxyhemoglobin are absent or occur in very different environments in carp hemoglobin. one ... | 1984 | 6329750 |
| the significance of cortisol for osmoregulation in carp (cyprinus carpio) and tilapia (sarotherodon mossambicus). | changes in plasma cortisol and glucose concentration were studied in carp during acclimation from fresh water (fw) to 1.5% salt water and vice versa. there was an increase in cortisol and glucose concentration during acclimation from fw to salt water which lasted for several days. reacclimation to fw did not cause clear changes in cortisol and glucose levels. one single injection of cortisol (0.2 mg/100 g or 1 mg/100 g) and additional transfer to salt water (1.5% for carp and 2.7% for tilapia) a ... | 1984 | 6329900 |
| dielectric studies on the reaction of the intracellular matter of fish red blood cells to detergent toxicity. | results from dielectric studies on the freshwater fish cyprinus carpio exposed to a sublethal concentration of sodium alkyl benzene sulphonate were compared with those from untreated control fish; recovery from the test solution was also checked. the influence of the anionic detergent on the intracellular matter of fish red blood cells was investigated by measurement of electrical conductivity and permittivity using a method that did not harm the cell membrane. the results were compared with a c ... | 1984 | 6330770 |
| intracellular distribution of fumarase in various animals. | the subcellular distribution of fumarase was investigated in the liver of various animals and in several tissues of the rat. in the rat liver, fumarase was predominantly located in the cytosolic and mitochondrial fractions, but not in the peroxisomal fraction. the amount of fumarase associated with the microsomes was less than 5% of the total enzyme activity. the investigation of the intracellular distribution of hepatic fumarase of the rat, mouse, rabbit, dog, chicken, snake, frog, and carp rev ... | 1984 | 6333419 |
| free extracellular [ca2+] at photoreceptor level equals that in vitreous in frog and carp eyes. | the extracellular ca2+ concentration has a strong influence on the function of retinal photoreceptors. in the present study both single- and double-barrelled ca2+-sensitive microelectrodes were used to measure the free calcium ion concentration in the extracellular fluid bathing the photoreceptors in opened excised eyes of frogs and carps. the electrode tip was localized electrophysiologically by voltage drops related to current pulses passed radially through the preparation, using the high-resi ... | 1984 | 6334664 |
| separation of lymphocyte subpopulations in carp cyprinus carpio l. by monoclonal antibodies: immunohistochemical studies. | lymphoid cell populations in various organs of the carp cyprinus carpio l. were investigated using a series of mouse monoclonal antibodies raised against carp thymocytes and carp serum ig. clones have been designated as ig+t+, ig+t- or ig-t+ on the basis of the reactivity with thymocytes and serum ig in the enzyme-linked immunosorbent assay (elisa) screening. their reaction to the lymphoid organs of carp was investigated on cryostat sections and cytocentrifuge slides using immunoperoxidase techn ... | 1983 | 6336715 |
| primary culture of normal pituitary cells of carp (cyprinus carpio) for the study of gonadotropin release. | a method for preparing enzymatically dispersed pituitary cell cultures of carp (cyprinus carpio) is described. the cultures have been used to assay a synthetic analog of gonadotropin releasing hormone (gnrh) and to determine the specificity of steroids able to affect gonadotropin (gth) release in vitro. time course secretion studies indicated that by 48 h incubation, in the presence of 500 pm gnrh, cumulative secretion of gonadotropin (719 ng +/- 90/2.5 x 10(5) cells) had exceeded that of contro ... | 1983 | 6337086 |
| identification of a heparin activated amidolytic enzyme in carp (cyprinus carpio) plasma. | a heparin activated amidolytic enzyme capable of cleaving synthetic thrombin sensitive chromogenic substrates was identified from the plasma of the carp (cyprinus carpio). activation was inhibited by kcl, protamine sulphate and human plasma. heparin was not required for the continued action of the enzyme. active enzyme was irreversibly inhibited by dfp. the ph and temperature optima was studied and the enzyme semi purified by sephadex g100 superfine and sephadex a50 chromatography. an approximat ... | 1983 | 6342205 |
| immunohistochemical demonstration of serotonin-containing csf-contacting neurons in the submammalian paraventricular organ. | the distribution and morphological aspects of the serotonin-containing neurons in the paraventricular organ of the carp, frog, turtle and chicken were studied by means of an immunoperoxidase technique using serotonin antiserum. in all species the serotonin-containing neurons were seen to have the appearance of the csf-contacting neurons and to be distributed in the pars ependymalis and the pars hypendymalis of the organ. particularly, in the frog, the serotonin-containing csf-contacting neurons, ... | 1983 | 6345483 |
| transport of proteins into mitochondria: a high conservation of precursor uptake and processing system. | ornithine transcarbamylase (ec 2.1.3.3) of rat (rattus norvegicus var. albus) liver, a urea cycle enzyme, is synthesized extramitochondrially as a larger precursor which is transported posttranslationally into mitochondria and processed to the mature enzyme. the precursor synthesized in vitro was taken up and processed to the mature enzyme by isolated pigeon (columba livia var. domestica) liver and frog (rana catesbeiana) liver mitochondria. carp (cyprinus carpio) liver mitochondria could also p ... | 1983 | 6347507 |
| high mutagenic potency of several polycyclic aromatic hydrocarbons induced by liver postmitochondrial fractions from control and xenobiotic-treated immature carp. | the metabolism of carcinogens in fish was examined by measuring the activation of different polycyclic aromatic hydrocarbons (pah) by carp (cyprinus carpio l.) liver post-mitochondrial fractions (s9) using the salmonella typhimurium ta100 reverse mutation assay. for this study, 1 non-carcinogen, anthracene (an), and 4 carcinogens, chrysene (chr), benzo[a]pyrene (bap), 3-methylcholanthrene (3mc) and 7,12-dimethylbenzanthracene (dmba), were chosen. the bioactivating potency of the metabolic system ... | 1983 | 6348528 |
| effect of 17 alpha-methyltestosterone, estradiol-17 beta and synthetic lhrh on production of gonadotropic hormone in pituitaries of rainbow trout (organ culture). | pituitary glands from 6-month-old sexually immature female rainbow trout, salmo gairdneri, were kept in organ culture for 48 or 72 h. certain groups of pituitaries were cultivated for 48 h on either control medium or medium with 17 alpha-methyltestosterone (mt), or with estradiol-17 beta (e2) in concentrations of 8.5 x 10(-7) m. other groups of pituitaries were cultivated for 72 h on control medium, or for 48 h on either control medium or mt-medium or e2-medium, and subsequently for 24 h on medi ... | 1983 | 6349817 |
| separation of cathepsin d-like proteinase and acid thiol proteinase of squid mantle muscle. | when the proteinases of the squid mantle muscle were extracted in the presence of dithiothreitol (dtt), the acid proteinase activity increased, indicating that the squid mantle muscle contains a considerable amount of the acid thiol proteinase. the crude extract hydrolyzed neither alpha-n-benzoyl-d,l-arginine-p-nitroanilide (bapa) nor azocasein, thus refuting the presence of cathepsins b and l in the mantle muscle. the cathepsin d-like proteinase and the acid thiol proteinase were separated by s ... | 1983 | 6349920 |
| ultrastructural immunocytochemistry of growth hormone cells in the goldfish pituitary gland. | the immunocytological activity of an antiserum to carp growth hormone was studied on the goldfish pituitary gland, using a peroxidase-antiperoxidase ultrastructural technique. the reaction was specific for secretory granules in cells in the proximal pars distalis categorized as growth hormone cells on the basis of ultrastructural characteristics. the results support the specificity of the antiserum for growth hormone and provide firm identification of the growth hormone cells in goldfish. | 1983 | 6350103 |
| purification and properties of enolase from carp (cyprinus carpio). comparison with enolases from mammals' muscles and yeast. | the enolase (2-phospho-d-glycerate hydrolyase e.c. 4.2.1.11) from carp muscle was obtained, the specific activity--88 u/mg of protein. km for 2-phosphoglycerate was 0.313 mm and for phosphoenolpyruvate--0.76 mm. the enzyme is active only in the presence of divalent metal ions, mg2+ being the best activator. the phosphate and fluoride decreased the activity of enzyme. the molecular weight of the dimeric form of enzyme was found to be 93,000. the enzyme is immunologically different from pig muscle ... | 1983 | 6352164 |
| [quantitative determination of spring viremia carp virus (svcv) antigens using immunofluorescence and immunoperoxidase plaque technic]. | 1983 | 6353812 | |
| immunochemical analysis of cod fish allergen m: locations of the immunoglobulin binding sites as demonstrated by the native and synthetic peptides. | the major allergen of codfish (allergen m) is a muscle protein belonging to the family of calcium binding parvalbumins. the primary structure of the molecule was established and the molecular weight was estimated from the sequence data to be 12,328. allergen m consists of 113 amino acid residues and one residue of glucose. a molecular arrangement of three domains (ab, cd and ef--the latter two bind one ca2+ ion each) was described for allergen m, analogous to carp parvalbumin pi 4.25. the sugges ... | 1983 | 6356964 |
| an immunofluorescence study on the occurrence of endogenous lectins in the differentiating oocytes of silver carp (hypophthalmichthys molitrix valenc.) and tench (tinca tinca l.). | 1984 | 6370924 | |
| carp preproinsulin cdna sequence and evolution of insulin genes. | the nucleic acid sequence of the preproinsulin cdna of carp (cyprinus carpio), cloned in the psti site of pbr322 ( liebscher et al. 1980), has been determined. the sequenced insert of 439 bp includes the complete coding information for carp preproinsulin (108 amino acids), 10 nucleotides of the 5'-and 105 nucleotides of the 3'-nontranslated regions. the nucleotide sequence confirms the previously established amino acid sequence of carp insulin ( makower et al. 1982) and determines those of the s ... | 1984 | 6373397 |
| effects of insulin on the fine structure of hepatocytes from winter-acclimatized carps: studies on protein synthesis. | male and female winter-acclimatized carps were injected with insulin. this treatment resulted in a sharp decrease in the liver glycogen content. although an increase in the ribosomal rna level was also observed, a cell-free system obtained from the hormone-treated fish exhibited less amino acid incorporation activity as compared to the control fish. however, polysomes from insulin-treated fish exhibited a higher amino acid incorporating activity when a soluble fraction of untreated winter carps ... | 1984 | 6376692 |
| monoclonal antibodies distinguish subtypes of retinal horizontal cells. | sixteen hybridomas have been identified that secrete antibodies specific to horizontal cells in the carp retina. the hybridomas have been classified into three groups based on their antibody staining patterns: group i, staining associated with all horizontal cells; group ii, staining associated with the most abundant subtype of horizontal cell (ch1); and group iii, staining associated with other subtypes of horizontal cells. most of the hybridomas fall in group ii; some of these antibodies stain ... | 1984 | 6385010 |
| clonorchis sinensis: life cycle, intermediate hosts, transmission to man and geographical distribution in korea. | the snail host of clonorchis sinensis in korea is referred to as parafossarulus manchouricus, but it has been recorded also as bulimus striatulus japonicus in literature published up to late 1950. the specific name of the snail was corrected such that bulimus striatulus of china, japan, and korea should be incorporated in the species of p. manchouricus. in general, the snail exists in limited areas of the rivers han, kum, mankyung, naktong, and yeongsan, and the infestation of the snail with the ... | 1984 | 6391501 |
| comparison of muscle proteinase activity among fish species. | the effects of temperature during storage, portion of muscle and growth stage of fish on the activity level of carp muscle acid (cathepsin d, ec 3.4.23.5), neutral and alkaline proteinases were examined. icing storage, freezer storage and portion of muscle did not affect each proteinase activity (p less than 0.05), but acid proteinase activity was affected by growth stage (p less than 0.05) and the level decreased from small to large fish. the activity levels of three kinds of proteinases were c ... | 1984 | 6391798 |
| [spore-forming bacteria in carp and rainbow trout, raised in waste waters from a steam power plant]. | 1984 | 6400797 | |
| rds-127 (2-di-n-propylamino-4,7-dimethoxyindane): central effects of a new dopamine receptor agonist. | apomorphine (apo), 2-di-n-propylamino-4,7-dimethoxyindane (rds-127) and 2-di-n-propylamino-5,8-dimethoxytetralin (jmc-181) were examined on a variety of biochemical and pharmacological assays to determine their possible interaction with dopamine (da) receptors. nanomolar concentrations of all three compounds displaced [3h]apo from specific high-affinity binding sites in rat striatal membrane preparations, while higher concentrations were required to displace [3h]spiperone or [3h]rauwolscine. apo ... | 1983 | 6401334 |
| dopamine modulates s-potential amplitude and dye-coupling between external horizontal cells in carp retina. | horizontal cells in the fish retina are electrically coupled and possess gap junctions so that intracellularly injected dye normally diffuses freely to neighbouring cells. applied dopamine (da) alters the spatial properties of the horizontal cell responses to light, increasing the amplitude of photopic l-type s-potentials but decreasing their lateral spread. these effects have been attributed to the action of da on horizontal cell membrane resistance, particularly at the gap junctions, and our p ... | 1983 | 6401844 |
| [free and peptide amino acid composition in the seminal fluid of the carp (cyprinus carpio)]. | free and peptidic amino acids were measured in the seminal plasma of carp. some acids such as glutamic acid and glutamine, taurine, glycine and proline were exceptionally abundant, while others like lysine, arginine and histidine were found in small amounts. generally, the amino acid level was higher in carp seminal plasma than in that of other vertebrate species. as this level was systematically higher than in the blood plasma, it is supposed that there was neoformation of an original fluid in ... | 1983 | 6404512 |
| the role of the retinal interplexiform cell: effects of 6-hydroxydopamine on the spatial properties of carp horizontal cells. | 1983 | 6405977 | |
| [potentiation by pimozide of the effects of lhrh-a on hypophyseal gonadotropin secretion, ovulation and spermiation in the common carp (cyprinus carpio)]. | during the reproductive season in august, we administered to adult male and female carp an lhrh analog (lhrh-a = d-trp6ea-lhrh) (50 micrograms/kg) alone or in association with pimozide, a dopamine inhibitor (10 mg/kg). lhrh-a caused the circulating portal level of gonadotropin to rise significantly in males and less in females. spermiation was stimulated in the males and oocyte maturation in females but there was no ovulation. an infection of pimozide and then of lhrh-a 6 h later significantly i ... | 1983 | 6405982 |
| [electrophysiological study of olfactory sensitivity in the carp]. | responses of single olfactory tract fibers cut off from prosencephalon to adequate stimulation of the carp olfactory epithelium revealed that the threshold sensitivity to aminoacids fell in the range between 10(-12) and 10(-10) m, that the magnitude and the form of the response depended on the concentration and type of stimulus as well as on the level of background activity. the results are compared with the biochemical data obtained earlier. | 1983 | 6406277 |
| comparative study of the glial fibrillary acidic protein in vertebrates by pap immunohistochemistry. | glial fibrillary acidic protein (gfa) has been visualized by direct peroxidase antiperoxidase (apa) immunohistochemistry in various vertebrates (cyclostomes, teleosts, amphibians, reptiles, birds, and several placental mammals). in this study gfa-immunoreactivity (gfa-i) was observed in all species examined except in cyclostomes and amphibians. two types of immunoreactive elements were observed: astrocytes and long processes without visible somata. astrocytic cells with gfa-i were first found in ... | 1983 | 6408144 |
| feeding great lakes fish to mink: effects on mink and accumulation and elimination of pcbs by mink. | the effects of feeding great lakes fish or fish products (carp, sucker, perch scraps, whitefish racks, and alewife fishmeal) to mink were studied. growth and furring of mink were normal for all species of fish tested. however, mink fed carp failed to reproduce, and the reproductive performance and/or kit survival in groups fed the perch, whitefish, and sucker were inferior to the control. only the alewife fishmeal diet supported reproduction and kit survival comparable to the control. pcb residu ... | 2013 | 6413698 |
| [gill structure in healthy carp]. | when the gills of carp are studied, this organ should be evaluated from there planes of projection: sagittal, transversal and horizontal. lamellae, the functional base of the gills, provide the exchange of oxygen between blood and water. lamellae consist of a network of sinuses with a skeleton formed by columnar cells and with walls constituted by the cytoplasmic processes of these cells. on the outer side the sinuses are covered with one or two layers of flat epithelial cells. the gill filament ... | 1983 | 6417877 |
| [phylogenesis of steroid-transport proteins in the blood: detection and characteristics of the sex-steroid-binding protein in the blood plasma of the carp]. | the interaction of 3h-5 alpha-dihydrotestosterone, 3h-progesterone, 3h-cortisol and 3h-testosterone with the cyprinus carpio blood proteins was studied by means of adsorption on the activated charcoal and equilibrium dialysis. protein which specifically binds androgen-, estradiol-, progesterone- and, to a lesser degree, corticosteroids was present in the blood plasma. the equilibrium constants of 3h-steroid association with this protein, the concentration off steroid-binding sites and its hormon ... | 2016 | 6419223 |
| a simple method for collecting 14co2 in fish. | a simple apparatus was designed for continuous collection of excreted 14co2 in fish. carp (cyprinus carpio; body wt. about 400 g) were intraarterially injected with [14c]acetate (44 mmol) and transferred to a test chamber (21 +/- 1 degrees c). within 48 h, 65.4 +/- 2.7% of the dose was recovered as 14co2. a small amount of 14co2 was retained in the water of the test chamber. control experiments using [14c]acetate-injected mice as '14co2-generators' showed that this 14c residue in the water could ... | 1983 | 6419401 |