Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| antibodies to tyvelose exhibit multiple modes of interference with the epithelial niche of trichinella spiralis. | infection with the parasitic nematode trichinella spiralis is initiated when the l1 larva invades host intestinal epithelial cells. monoclonal antibodies specific for glycans on the larval surface and secreted glycoproteins protect the intestine against infection. protective antibodies recognize tyvelose which caps the target glycan. in this study, we used an in vitro model of invasion to further examine the mechanism(s) by which tyvelose-specific antibodies protect epithelial cells against t. s ... | 2000 | 10722582 |
| the genetic analysis of f1 hybrid larvae between female trichinella spiralis and male trichinella britovi. | hybrids between female trichinella spiralis and male trichinella britovi were constructed. then, hybrid genotype was characterized by dna markers including mitochondrial cytochrome c oxidase subunit i (co i) gene, the gene encoding the 43-kda excretory-secretory (es) protein, and genomic dna fragments specific for t. spiralis and t. britovi identified from random amplified polymorphism dna (rapd). pcr-restriction fragment length polymorphism (pcr-rflp) analysis of the mitochondrial co i gene rev ... | 2000 | 10725692 |
| immune response to trichinella spiralis larvae after treatment with the anti-allergic compound ketotifen. | ketotifen was used as an anti-allergic agent to study the relationship between eosinophil-related responses and igg1 and igg2a antibody responses in balb/c mice infected with trichinella spiralis. the results showed that leukocyte and eosinophil numbers and interleukin-5 (il-5) concentrations in the peritoneal fluid increased after exposure to nematodes and the increases were slightly greater in animals treated with ketotifen. a decreased concentration of eosinophil peroxidase and an elevation i ... | 2000 | 10726994 |
| nitric oxide mediates intestinal pathology but not immune expulsion during trichinella spiralis infection in mice. | the relationship between intestinal pathology and immune expulsion of gastrointestinal (gi) nematodes remains controversial. although immune expulsion of gi helminth parasites is usually associated with th2 responses, the effector mechanisms directly responsible for parasite loss have not been identified. we have previously shown that while the intestinal pathology accompanying the expulsion of the gi parasite trichinella spiralis may be dependent on il-4 and mediated by tnf, parasite loss is in ... | 2000 | 10754319 |
| schistosoma japonicum in the black rat, rattus rattus mindanensis, from leyte, philippines in relation to oncomelania snail colonies with reference to other endoparasites. | this study examined the prevalence of schistosoma japonicum infections in field rats, rattus rattus mindanensis, according to different trapping locations. between october 1995 and january 1996, traps were set in the municipality of palo, leyte, philippines to determine the correlation of rats infected with schistosomiasis to the proximity of the intermediate snail host, oncomelania hupensis quadrasi, colonies. of the 22 rats that were caught within a snail colony, 21 (95.5%) were positive for s ... | 1999 | 10774707 |
| larvae-induced plasma membrane wounds and glycoprotein deposition are insufficient for trichinella spiralis invasion of epithelial cells. | trichinella spiralis l1 larvae infect susceptible hosts by invading epithelial cells that line the small intestine. invasion also occurs in vitro when larvae are inoculated into cultures of epithelial cells from several different animal species. to further investigate the mechanism of invasion, we studied the interaction of larvae with the rat epithelial cell line iec-6. larvae did not invade iec-6 cells, but did cause the cells to take up parasite glycoproteins. glycoprotein bearing cells remai ... | 2000 | 10779597 |
| modulation of the pathologic and apoptotic changes of experimental toxoplasmosis by concomitant infection with trichinella spiralis. | this study shows that toxoplasma gondii infection is capable of inducing a process of programmed cell death not only in the infected hepatocytes of experimental murine model, but also the neighbouring non-infected cells. however, some cells showed a possible mixed apoptotic and necrotic pattern. surprisingly, lymphocytes and kupffer cells in portal sinusoids nearby the parasite vacuoles showed no apparent changes. toxoplasma in concomitant infection with a different parasite, trichinella spirali ... | 2000 | 10786020 |
| the effect of antioxidant preparation (antox) on the course and efficacy of treatment of trichinosis. | this study evaluated the effect of administration of exogenous antioxidant (antox) on the course and treatment of trichinella spiralis in the intestinal phase (2 days post infection, p.i.) and the muscle phase (35 days p.i.) using albino rats as an experimental model. the results indicated that the administration of antox had led to delayed expulsion of adult worms from the small intestine, decreased inflammatory reactions in the intestines and muscles and causing an increase in the muscle larva ... | 2000 | 10786041 |
| murine model study of the practical implication of trypanosome-induced immunosuppression in vaccine-based disease control programmes. | the relevance of trypanosome-induced immunosuppression in relation to the efficacy of vaccine-induced immunity was studied in mice. mice were immunised with crude trichinella spiralis muscle larvae homogenate vaccine and infected with t. spiralis and/or trypanosoma brucei. vaccination significantly decreased adult worm burden (p<0. 05) and accelerated worm expulsion in mice infected with t. spiralis only. t. brucei superinfection resulted in monocytosis, suppressed eosinophilia, significant decr ... | 2000 | 10802294 |
| efficient synthesis of 3, 6-dideoxy-beta-d-arabino-hexopyranosyl-terminated lacdinac glycan chains of the trichinella spiralis parasite. | the synthesis of a linear trisaccharide epitope of the trichinella spiralis n-linked glycan, in a form amenable to glycoconjugate formation, is reported. the trisaccharide contains the synthetically challenging lacdinac [beta-galpnac(1-->4)-beta-glcpnac] element, as well as a terminal 3,6-dideoxy-beta-d-arabino-hexopyranose (tyvelose) residue. an orthogonal protection strategy is described, which permits the protection and manipulation of three amino groups present in the disaccharide beta-galna ... | 2000 | 10814198 |
| a study of proteases throughout the life cycle of trichinella spiralis. | in the present report we study the proteolytic activity of the excretion-secretion and crude extracts of different stages of trichinella spiralis (owen, 1835) railliet, 1895, (muscle-stage larvae, adult worms before and after mating, and newborn larvae) using natural substrates (structural and hematic mammalian proteins). the analysis of the results allow us to set up a certain stage-specificity, as well as an important relationship between the protease patterns throughout the parasite life cycl ... | 2000 | 10833016 |
| analysis of intestinal intraepithelial lymphocyte populations in experimental trichinella spiralis infection of mice. | the potential role of intestinal intraepithelial lymphocytes (i-iels) in the generation of host protective immunity after helminth infection was investigated using the trichinella spiralis (owen, 1835)/mouse model. in this study we found a significant rise of tcrgamma(delta)+ i-iels (p < 0.001) concurrent with the jejunal goblet cells (gc) hyperplasia in t. spiralis-infected c57bl mice on day 4 p.i. however, no direct relationship between the kinetics of the increase in tcrgamma(delta)+ i-iels a ... | 2000 | 10833017 |
| single-stranded endonuclease activity in the excretory--secretory products of trichinella spiralis and trichinella pseudospiralis. | a novel acidic extracellular single-stranded endonuclease was demonstrated for the first time in the excretory-secretory (e-s) products of 2 species of trichinella. unlike the double-stranded endonuclease reported earlier, the single-stranded molecule is divalent cation independent and is detected in both t. spiralis and t. pseudospiralis e-s products. it hydrolysed single-stranded dna and rna at comparable rates. the single-stranded endonuclease was sensitive to inhibition by zn2+ and to high c ... | 2000 | 10840982 |
| inhibitory effects of human neutrophil functions by the 45-kd glycoprotein derived from the parasitic nematode trichinella spiralis. | we evaluated the effect of the 45-kd protein of trichinella spiralis (gp45), purified by affinity chromatography, on random migration and chemotaxis, the oxidative metabolism of human neutrophils and on the cd11b upregulation induced by formyl-methionyl-leucyl-phenylalanine (f-mlp). | 2000 | 10859470 |
| senescent jejunal mast cells and eosinophils in the mouse preferentially translocate to the spleen and draining lymph node, respectively, during the recovery phase of helminth infection. | because mice infected with trichinella spiralis experience a pronounced, but transient, mastocytosis and eosinophilia in their intestine, this disease model was used to follow the fate of senescent t cell-dependent mast cells (mcs) and eosinophils. very few mcs or eosinophils undergoing apoptosis were found in the jejunum during the resolution phase of the infection, even though apoptotic mcs were common in the large intestine. although the mesenteric draining lymph nodes contained large numbers ... | 2000 | 10861071 |
| influence of temperature on the survival and infectivity of trichinella spiralis larvae in sarcophaga argyrostoma (diptera, sarcophagidae) maggots. | to investigate the role of fleshfly maggots as a paratenic host for trichinella spiralis larvae, maggots of sarcophaga argyrostoma (muscidae, sarcophagidae) kept at different temperatures (26, 22, 20, 16, 12, 8, and 4 c) were allowed to feed on t. spiralis-infected mouse meat. trichinella larvae found in maggots kept at 8-26 c were able to cause infection when inoculated in mice. infective larvae survived in maggots up to 5 days postinfection at 8 c and for shorter periods of time at higher temp ... | 2000 | 10864271 |
| detection of heat shock protein-70 from trichinella spiralis larvae using a modification of the routine western blotting procedure. | this is the first study that establishes a standardized western blotting method for the detection of heat shock protein (hsp) 70 from trichinella spiralis using (selected) monoclonal antibodies (mabs). enhancement of hsp transfer onto the supportive membrane and increased retention of protein by the membrane are prominent features of the procedure. the reactivity of t. spiralis hsp70 on western blots was substantially increased by the use of a 10% acrylamide gel, the optimization of conditions d ... | 2000 | 10864273 |
| trichinella spiralis and trichinella pseudospiralis: developmental patterns of enzymes involved in thymidylate biosynthesis and pyrimidine salvage. | thymidylate synthase, dihydrofolate reductase and dutpase specific activities were found to remain at a high and constant level in crude extracts from adult worms of trichinella spiralis, as well as from muscle larvae of both trichinella spiralis (isolated 1-24 months after infection) and trichinella pseudospiralis (isolated 5.5-13 months after infection). the results obtained with trichinella pseudospiralis muscle larvae isolated with the use of pepsin did not differ from those obtained when pe ... | 2000 | 10874722 |
| blinded, placebo-controlled trial of antiparasitic drugs for trichinosis myositis. | there is no consensus on the benefits of treatment with any specific anthelminthic compound on muscle-stage trichinosis. a double-blind, placebo-controlled comparison was done of 3 antiparasitic drugs during an outbreak of trichinosis in chiangrai province, northern thailand. forty-six adults were randomized to receive 10 days of oral treatment with mebendazole (200 mg twice a day), thiabendazole (25 mg/kg twice a day), fluconazole (400 mg initially, then 200 mg daily), or placebo. all patients ... | 2000 | 10882628 |
| [standardization of elisa igm and iga for immunodiagnosis of human trichinosis]. | an elisa test for trichinosis using as antigen a larvae soluble fraction from trichinella spiralis was carried out for the detection of igm and iga specific antibodies in 45 serum samples from patients confirmed or suspected to have trichinosis by strong clinical and epidemiological evidences. all the patients had positive serology detected by precipitin test, bentonite floculation test, indirect hemagglutination test and elisa igg test. the cut-off value was determined using two criteria. crite ... | 1999 | 10883499 |
| clinical features of experimental trichinellosis in the raccoon dog (nyctereutes procyonoides). | three groups of six raccoon dogs (nyctereutes procyonoides) were provided for the experiment: the first group was infected with pig-origin trichinella spiralis, the second with raccoon dog-origin trichinella nativa, and the third served as controls. infection dose for both parasite species was 1000 larvae/kg of body weight, which led to intense final infection. clinical signs, haematology and serum biochemistry with repeated blood samples were monitored up to 12 weeks post-infection. the most si ... | 2000 | 10889361 |
| inhibition of the protective iga response by ketotifen is related to the inflammatory reaction in the peritoneal cavity and intestinal mucosa of balb/c mice infected with trichinella spiralis. | interleukin-5 (il-5) production, eosinophilia, and iga responses of balb/c mice infected with trichinella spiralis were measured in the peritoneal cavity and intestinal mucosa. ketotifen, an anti-allergic compound, was used to control the inflammatory response. iga responses differed against adult and muscle stages of the parasite and between the intestine and the peritoneal cavity. il-5 and eosinophil production also differed between the intestine and the peritoneal cavity. the occurrence of pa ... | 2000 | 10894474 |
| beta7 integrin-deficient mice: delayed leukocyte recruitment and attenuated protective immunity in the small intestine during enteric helminth infection. | the ontogeny and function of gut-associated-lymphoid tissue is known to be critically dependent on the beta7 integrin subfamily. we have investigated the development of intestinal inflammation and pathogen-specific protective immunity to enteric helminth infection in beta7 integrin knockout (ko) mice. during trichinella spiralis infection of the small intestine there was a significant delay and reduction in the magnitude of intestinal eosinophilia and mastocytosis in the absence of p7 integrin, ... | 2000 | 10898502 |
| the first human case of trichinella spiralis infection in korea. | three cases of human infection by trichinella spiralis were first confirmed by detecting encysted larvae in the biopsied muscle in december 1997, in korea. the patients were one 35- and two 39-year-old males residing in kochang-gun, kyongsangnam-do. they had a common past history of eating raw liver, spleen, blood and muscle of a badger, meles meles melanogenys, and complained of high fever, facial and periorbital edema, and myalgia. hematologic and biochemical examinations revealed leukocytosis ... | 2000 | 10905075 |
| sylvatic trichinellosis in southwestern spain. | the epidemiology of trichinella spp. in their main sylvatic hosts, wild boar (sus scrofa ferus and red fox (vulpes vulpes), in extremadura (southwestern spain) was studied. we examined 88 trichinella spp.-positive wild boar muscle-tissue samples from a total of 29,333 killed animals, referred to the veterinary parasitology department (university of extremadura, spain) by the extremadura veterinary service. additionally, 227 red foxes killed during the hunting season and thus not subject to veter ... | 2000 | 10941739 |
| proteolytic enzymes secreted by larval stage of the parasitic nematode trichinella spiralis. | excretory/secretory products (es), collected from in vitro cultures of muscle larvae (l1) of trichinella spiralis (owen, 1835) were examined for the presence of proteolytic enzymes. several discrete proteinases in the size range of 25-55 kda were identified by substrate gel electrophoresis and were characterised according to ph optima, substrate specificity and inhibitor sensitivity using azocasein assay. serine, cysteine and metalloproteinases active at ph 5-7 were identified. the serine protei ... | 2000 | 10945739 |
| partial characterization of serine proteinases secreted by adult trichinella spiralis. | serine proteinases secreted by adult trichinella spiralis were isolated from excretory/secretory products (es) of in-vitro-cultured parasites by affinity chromatography with p-benzamidine-celite. the purified enzymes had molecular weights of approximately 18, 40, and 50 kda and displayed enzyme activity against a range of low-molecular-weight substrates, gelatin, and azocasein. the antigenicity of these parasite proteinases was demonstrated by the inhibition of enzymatic activity with igg purifi ... | 2000 | 10952270 |
| comparison of igg3 responses to carbohydrates following mouse infection or immunization with six species of trichinella. | the igg3 antibody responses to carbohydrate epitopes were compared in balb/c mice infected or immunized with six species of trichinella: t. spiralis (t1), t. nativa (t2), t. britovi (t3), t6, t. nelsoni (t7), and t8. the dynamics of igg3 responses and antigen recognition following infection or immunization were measured by elisa and western blot respectively, using glycosylated and deglycosylated larval crude extracts (lce) prepared from homologous isolates. a high degree of protein glycosylatio ... | 2000 | 10953221 |
| genetic diversity among isolates of trichinella spiralis from the province of buenos aires, argentina. | random amplified polymorphic dnas, (rapds) are used to study the occurrence of trichinella britovi and t5 among domestic animals in the province of buenos aires, argentina and to assess the genetic diversity among isolates of t. spiralisfrom this area in a number of infected hosts. all the local isolates proved to be t. spiralis. six of the eight primers used indicate that the buenos aires isolates are distinct from each other as they produce a considerable number of polymorphic bands. our overa ... | 2000 | 10953229 |
| experimental trichinellosis in reindeer. | six female reindeer calves were inoculated intraruminally with various doses of trichinella muscle larvae. four calves were inoculated with t. nativa, receiving 15,000 (n = 1), 5,000 (1), and 2,500 (2) larvae each. two calves were inoculated with 5,000 t. spiralis larvae each. blood samples were collected twice per week for total white blood cell (wbc) and differential counts and for serology using enzyme-linked immunosorbent assay (elisa) based on t. spiralis excretory-secretory antigen. on day ... | 2000 | 10958453 |
| characterization of the n-linked glycans of adult trichinella spiralis. | 2000 | 10960176 | |
| in vivo changes in the intestinal reflexes and the response to cck in the inflamed small intestine of the rat. | functional motor changes and morphological alterations have been associated with intestinal inflammation. the aim of our study was to evaluate functional alterations of intestinal reflexes and of the responses to cck in the trichinella spiralis model of intestinal inflammation. rats were prepared with strain gauges and electrodes in the small intestine to evaluate spontaneous motor activity, the ascending contraction of the peristaltic reflex, and the motor responses to cck-8 infusion. infected ... | 2000 | 10960353 |
| serological evaluation of thin-layer immunoassay-enzyme-linked immunosorbent assay for antibody detection in human trichinellosis. | a new immunoenzymatic test, named the thin-layer immunoassay-enzyme-linked immunosorbent assay (tia-elisa), was evaluated for antibody detection in human trichinellosis using excretion and secretion products prepared from trichinella spiralis muscle larvae. serum samples from people with positive muscle biopsies or symptoms compatible with the disease (n = 8 or 26, respectively), all reactive in enzyme-linked immunoelectrotransfer blot assay (eitb), as well as 67 serum samples from healthy, eitb ... | 2000 | 10973459 |
| [the organ pathology in the acute stage of tissue helminthiases: the role of eosinophilia of the blood and tissues, blood immunoglobulins e and g4 and immune response-inducing factors]. | the pathogenesis of organic lesions in the early phase of tissue-dwelling helminthic infections has been analyzed and discussed by taking into account the previous findings [8, 9, 12], the results of current clinical and immunological studies [10, 11, 60] in the light of a fundamental literature data review. the products secreted by invasive helminthic larvae and the substances expressed onto their cuticula tegument surface mimic nonspecific [34, 43] and specific [21, 28, 33, 42] modulators of h ... | 2000 | 10981403 |
| [the synthesis and study of the trichinellacidal activity of bromine and chlorine derivatives of 8-quinolyloxysalicylanilides]. | some new bromine (chlorine) derivatives of 8-quinolyloxysalicylanilides were synthesized and tested for trichinellacidal activity. among them there was the substance n-[3-bromophenyl-4-(5-chloroquinolinoxy)]-3,5-dibromosalicylami de which exhibited its high trichinellocidal activity (in albino mice infected with decapsulated trichinella spiralis) that was close to that of mebendazole. | 2000 | 10981410 |
| phosphorylcholine-containing n-glycans of trichinella spiralis: identification of multiantennary lacdinac structures. | although the presence of phosphorylcholine (pc) in trichinella spiralis is well established, the precise structure of the pc-bearing molecules is not known. in this paper, we report structural studies of n-glycans released from t.spiralis affinity-purified antigens by peptide n-glycosidase f. three classes of n-glycan structures were observed: high mannose type structures; those which had been fully trimmed to the trimannosyl core and were sub-stoichiometrically fucosylated; and those with a tri ... | 2000 | 10988255 |
| a parasitological survey of wild red foxes (vulpes vulpes) from the province of guadalajara, spain. | an epizootiological survey of leishmaniosis, coccidiosis and parasitic helminths in 67 foxes (vulpes vulpes) was conducted in guadalajara (central spain). examination for parasitic protozoa revealed prevalences of 74% leishmania (determined by molecular methods) and 2.9% coccidia oocysts (fecal flotation). survey of parasitic helminths (fecal flotation/necropsy) demonstrated the presence of nine species, including six nematodes, two cestodes and one trematode. nematodes were the most common para ... | 2000 | 10996735 |
| cytotoxicity-blocking antibodies in human chronic trichinellosis. | antisurface newborn larva (nbl) antibodies (abs) were found in sera from individuals chronically infected with trichinella spiralis. these abs were incapable of inducing nbl death by activation of normal human leukocytes of peripheral blood as determined by in vitro assays of antibody-dependent cell cytotoxicity (adcc). besides, such sera blocked the cytotoxic reaction mediated by abs produced a few weeks after infection. the blocking activity could not be attributed to any particular isotype by ... | 2000 | 11002986 |
| outbreak of suspected trichinosis among travelers returning from a neighboring island. | an outbreak of trichinosis involving a cohort of 33 returning travelers from a resort island in a neighboring country was suspected, beginning with 2 initial cases who were hospitalized with a syndrome of fever, myalgia and eosinophilia. | 2000 | 11003731 |
| interleukin-5 deficient mice exhibit impaired host defence against challenge trichinella spiralis infections. | enteric nematode infections are characterized by both peripheral and tissue eosinophilia. the cytokine interleukin (il)-5 is considered a critical factor in the proliferation and recruitment of eosinophils, however, studies suggest it plays little role in host defence, at least during primary trichinella spiralis infections. less is known concerning its role in host defence or in the inflammatory response that develops against challenge infections with the same parasite. we examined these questi ... | 2000 | 11012974 |
| pork program seeks to quiet public fear, increase foreign competitiveness. | 2000 | 11019694 | |
| [cloning and characteristics of rapd-markers from the genome of the parasitic nematodes trichinella spiralis and trichinella pseudospiralis]. | 2000 | 11033809 | |
| evidence of opiates and opioid neuropeptides and their immune effects in parasitic invertebrates representing three different phyla: schistosoma mansoni, theromyzon tessulatum, trichinella spiralis. | studies done in our laboratories have demonstrated that the parasitic trematode, schistosoma mansoni is capable of producing several proopiomelanocortin (pomc) peptides including beta-endorphin, adrenocorticotropin (acth), melanocyte stimulating hormone (alphamsh) and enkephalin as well as morphine. some of these opioids have been demonstrated to be immunosuppressive and may play an important part in immune evasion by these parasites. the parasitic nematode trichinella spiralis also produces imm ... | 2000 | 11034157 |
| dna fingerprints of trichinella as revealed by restriction fragment length polymorphism and single-strand conformational polymorphism (rflp-sscp). | a method was developed for gene fingerprinting, combining the principles of restriction fragment length polymorphism and single-strand conformational polymorphism (pcr-rflp-sscp). taking advantage of this method, we analysed the genotypes of 20 isolates from five species of trichinella (trichinella spiralis, trichinella britovi, trichinella nativa, trichinella murrelli and trichinella pseudospiralis) and two uncertain genotypes (trichinella t6 and trichinella t8). target genes for the analysis i ... | 2000 | 11040092 |
| a reversible protein phosphorylation system is present at the surface of infective larvae of the parasitic nematode trichinella spiralis. | trichinella spiralis infective larvae have externally oriented enzymes catalysing reversible protein phosphorylation on their surface. incubation of larvae with exogenous atp resulted in phosphorylation of surface bound and released proteins. exposure of the parasites to bile, a treatment which renders them infective for intestinal epithelia, resulted in increased release of protein and an altered profile of phosphorylation. both serine/threonine and tyrosine phosphorylation and dephosphorylatio ... | 2000 | 11042262 |
| [trichinelliasis in 1998]. | the decreasing problem in human trichnellosis still can be observed during last years in poland. the presence of trichinella endemic areas in the north-east part of the country still determines the necessity of the vary careful the meat survey against trichinella spiralis. | 2000 | 11076160 |
| aging and resistance to trichinella spiralis infection following xenobiotic exposure. | aging is accompanied by well-documented physiological changes, including alterations in the immune system that can lead to reduced resistance to a variety of infectious agents. we tested the hypothesis that immunosenescence exacerbates the immunosuppressive effect of xenobiotics. if proven true, a given dose of an immunosuppressive xenobiotic would cause greater suppression of host resistance in an aged population. | 2000 | 11083112 |
| five types of nuclei present in the capsule of trichinella spiralis. | this study describes morphological changes during capsule formation of trichinella spiralis with emphasis on nuclei within the capsule. the nuclei of the pre-existing muscle cell may become hypertrophied or degrade soon after the entrance of newborn larvae. cytoplasm associated with these hypertrophied nuclei was basophilic in h&e staining and composed of mitochondria and rough endoplasmic reticulum. satellite cells underwent cell division and joined to the nurse cell. these nuclei may also beco ... | 2000 | 11085240 |
| historical perspectives and current global challenges of trichinella and trichinellosis. | trichinella spiralis and related species of trichinella have had a long history of causing human disease, and as a foodborne pathogen have had a major impact on international commerce of pork and other meat animal species which are known to transmit the parasite. our knowledge of trichinella has increased substantially over the past few years particularly in the areas of phylogeny, host diversity, epidemiology and control. in this paper, we provide a brief overview of our understanding of trichi ... | 2000 | 11099836 |
| epidemiology of trichinellosis in mexico, central and south america. | trichinella species are widely distributed throughout the world and are found in a large number of carnivorous animals, humans and incidental hosts. the data presented in this review show that trichinella infection has been reported in both humans and animals in mexico, argentina and chile since the end of the 19th century, and more recently in bolivia. this parasitic infection is still a public health problem in countries such as argentina and chile. although efforts have focused on the control ... | 2000 | 11099838 |
| epidemiology of trichinellosis in asia and the pacific rim. | the epidemiology of trichinellosis, species of trichinella present and the food and eating habits of people affected in asia and the pacific rim are reviewed with emphasis on japan, china and thailand. trichinella seems to be prevalent throughout this region although outbreaks of trichinellosis have not been reported in some areas. major outbreaks of the disease have been reported primarily in china and thailand. this is the result of three factors: (1) china and thailand are highly endemic area ... | 2000 | 11099839 |
| factors affecting the flow among domestic, synanthropic and sylvatic cycles of trichinella. | nematodes of the genus trichinella are maintained in nature by sylvatic or domestic cycles. the sylvatic cycle is widespread on all continents, from frigid to torrid zones, and it is maintained by cannibalism and scavenging behavior of carnivores. trichinella is primarily a parasite of carnivorous mammals, although one non-encapsulated species, trichinella pseudospiralis, has also been detected in birds. the anaerobic metabolism of larvae in nurse cells allows their survival in extremely decayed ... | 2000 | 11099840 |
| host diversity and biological characteristics of the trichinella genotypes and their effect on transmission. | the host spectra and biological diversity of the trichinella genotypes are reviewed. while all genotypes appear to reproduce equally well in carnivore hosts, their infectivity and persistence in omnivores and herbivores show remarkable differences. most of the genotypes found in wildlife have low infectivity for pigs and some persist only for a few weeks; in herbivores this tendency is even more profound, but malnourished, environmentally stressed, or otherwise immuno-suppressed hosts are likely ... | 2000 | 11099841 |
| dynamics of the igg3 responses following immunisation of balb/c mice with somatic and excretory/secretory antigens from various trichinella species. | comparison of the dynamics and antigen recognition profiles of igg3 following immunisation with larval crude extracts (lce) and excretory-secretory (es) products from muscle larvae of different species of trichinella (t. spiralis, t. nativa, t. britovi, t. nelsoni and genotype t6) was made in balb/c mice. high levels of gg3 response were obtained in elisa following immunisation with lce from all species with maximum levels achieved between days 59 and 64 post-immunisation (p.i.) and maintained u ... | 2000 | 11104144 |
| sero-surveillance of wild boar in the netherlands, 1996-1999. | from 1996 to 1999, blood samples were collected from wild boar shot during the hunting season in crown properties, national parks and the free wildlife belt in the netherlands. sera were screened for the presence of antibodies against classical swine fever virus (csfv), swine vesicular disease virus (svdv), aujeszky's disease virus (adv) and trichinella spiralis. the results of the sero-surveillance system indicate that csfv, svdv and adv are uncommon within the wild boar population. hence, the ... | 2000 | 11107628 |
| interstitial cells of cajal and inflammation-induced motor dysfunction in the mouse small intestine. | interstitial cells of cajal (icc) play an important role in the control of gastrointestinal motility. we aimed to determine a potential role for icc in the pathophysiology of inflammation-induced motor disorders. | 2000 | 11113080 |
| interleukin-10 is an unequivocal th2 parameter in the rat, whereas interleukin-4 is not. | exposure of wistar rats to the immunotoxic compounds hexachlorobenzene (hcb), bis(tri-n-butyltin)oxide, and benzo(a)pyrene was previously found to affect mrna expression of interleukin (il)-2, il-2r alpha-chain, and interferon (ifn)-gamma, the prototypic th1 cytokine. in contrast, the mrna expression of il-4, the prototypic th2 cytokine, was unaffected. this latter finding suggested that the il-4 mrna expression may not be an unequivocal parameter for th2 responses in the rat. in order to obtain ... | 2000 | 11119253 |
| delayed expulsion of the nematode trichinella spiralis in mice lacking the mucosal mast cell-specific granule chymase, mouse mast cell protease-1. | expulsion of gastrointestinal nematodes is associated with pronounced mucosal mast cell (mmc) hyperplasia, differentiation, and activation, accompanied by the systemic release of mmc granule chymases (chymotrypsin-like serine proteases). the beta-chymase mouse mast cell protease-1 (mmcp-1) is expressed predominantly by intraepithelial mmcs, and levels in the bloodstream and intestinal lumen are maximal at the time of worm expulsion in parasitized mice. to address the in vivo functions of mmc-spe ... | 2000 | 11120781 |
| immunopathology of intestinal helminth infection. | the relationship between intestinal pathology and immune expulsion of gastrointestinal nematodes remains controversial. parasite expulsion is associated with intestinal pathology in several model systems and both of these phenomena are t cell dependent. however, while immune expulsion of gastrointestinal helminth parasites is usually associated with th2 responses, the effector mechanisms directly responsible for parasite loss have not been elucidated. in contrast, the intestinal pathology observ ... | 2000 | 11123752 |
| immunoelectrotransfer blot assay in acute and chronic human trichinellosis. | an immunoelectrotransfer blot assay (ietb) using excretory secretory products of muscle larvae of trichinella spiralis (ml-esp) and the avidin biotin system was developed in order to characterize reactivity against ml-esp in sera from patients with acute and chronic trichinellosis. a complete pattern of up to 13 bands was developed by sera from individuals with trichinellosis where doublets, triplets, or single bands were shown to have molecular weights of roughly 66, 55, 45, 36, 29, 24, and 14 ... | 2000 | 11128491 |
| prior immunity to trichinella spiralis prevents (re)occurrence of an explicit stress response in intestines but not in mesenteric lymph nodes, heart and lungs from reinfected rats. | we recently showed that, in our trichinella spiralis rat model, first exposure, but not re-exposure to infective-stage larvae evoked heat shock responses in 4 test organs. our work, however, failed to implicate either early complete clearance of challenge muscle larvae (ml), or rapid elimination of newborn larvae (nbl) in the phenomenon noted in reinfected rats. this study clarifies that issue using 2 established facts in t. spiralis biology and anti-t. spiralis immunology. that is, adult worms ... | 2000 | 11128808 |
| neutrophil migration stimulates rat intestinal epithelial cell cytokine expression during helminth infection. | we are interested in understanding the role of epithelial cells during inflammation, and we previously reported that rat small intestinal epithelial cells express interleukin-1beta (il-1beta) during infection by trichinella spiralis. we now report that the epithelium also produces the potent neutrophil chemotactic factor, macrophage inflammatory protein-2 (mip-2), and an il-1 antagonist: the type ii il-1 receptor. consequently we investigated the pattern of neutrophil infiltration into the infec ... | 2000 | 11129649 |
| synergistic action of a cyclic depsipeptide and piperazine on nematodes. | the present study describes the synergistic effects of the cyclic depsipeptide bay 44-4400 and piperazine in the treatment against the nematodes trichinella spiralis, heligmosomoides polygyrus, and heterakis spumosa. the in vitro anthelmintic activity of a combination of the two compounds (1.7 motility units) against t. spiralis larvae was significantly higher than the sum of the individual drug effects (1.3 motility units). with regard to the rate of expulsion of h. polygyrus worms from the int ... | 2000 | 11133114 |
| stat6 dependent goblet cell hyperplasia during intestinal nematode infection. | to identify the role of signal transducer and activator of transcription factor 6 (stat6) in the development of intestinal goblet cell hyperplasia during nematode infection, we compared the number of goblet cells in stat6 deficient (stat6 -/-) mice with that generated in wild-type (stat6 +/+) mice in trichinella spiralis infection. the number of goblet cells significantly increased with infection in wild-type mice. however, stat6 -/- failed to generate infection-induced goblet cell hyperplasia a ... | 2001 | 11136476 |
| mucosal immunity against parasitic gastrointestinal nematodes. | the last two decades witnessed significant advances in the efforts of immunoparasitologists to elucidate the nature and role of the host mucosal defence mechanisms against intestinal nematode parasites. aided by recent advances in basic immunology and biotechnology with the concomitant development of well defined laboratory models of infection, immunoparasitologists have more precisely analyzed and defined the different immune effector mechanisms during the infection; resulting in great improvem ... | 2000 | 11138315 |
| trichinella spiralis mtdna: a nematode mitochondrial genome that encodes a putative atp8 and normally structured trnas and has a gene arrangement relatable to those of coelomate metazoans. | the complete mitochondrial dna (mtdna) of the nematode trichinella spiralis has been amplified in four overlapping fragments and 16,656 bp of its sequence has been determined. this sequence contains the 37 genes typical of metazoan mtdnas, including a putative atp8, which is absent from all other nematode mtdnas examined. the genes are transcribed from both mtdna strands and have an arrangement relatable to those of coelomate metazoans, but not to those of secernentean nematodes. all protein gen ... | 2001 | 11156984 |
| detection and identification of eight trichinella genotypes by reverse line blot hybridization. | a reverse line blot (rlb) assay was developed to identify different trichinella genotypes. the rlb assay accomplishes detection and specific identification of the different trichinella genotypes and relies on hybridization of the amplified 5s ribosomal dna intergenic spacer regions to specific, membrane-bound oligonucleotide probes. after one single amplification, we were able to detect and genetically identify six sibling species, i.e., t. spiralis, t. britovi, t. nativa, t. murrelli, t. nelson ... | 2001 | 11158122 |
| critical role for signal transducer and activator of transcription factor 6 in mediating intestinal muscle hypercontractility and worm expulsion in trichinella spiralis-infected mice. | intestinal nematode infections in rats or mice are accompanied by intestinal muscle hyper contractility that may contribute to parasite expulsion from the gut. previous studies demonstrated that both the expulsion of nematode parasites and the associated muscle hyper contractility are dependent on cd4(+) t helper cells. nevertheless, the precise immunological mechanism underlying changes in intestinal muscle function remains to be determined. in this study, we investigated the role of interleuki ... | 2001 | 11159976 |
| failure of mebendazole in the treatment of humans with trichinella spiralis infection at the stage of encapsulating larvae. | trichinella spiralis larvae infective for laboratory mice were collected from muscle biopsies performed at different times (from 1 day to 16 months) following the end of treatment, indicating the failure of mebendazole to kill trichinella parasites when they are encapsulating in muscles. | 2001 | 11181129 |
| the detection and occurrence of circulating antigens of trichinella spiralis during worm development. | four enzyme-based immunoassays were compared in detecting circulating antigens (ca) of trichinella spiralis, i.e. microfluorescence, dissociated enhanced lanthanide fluoroimmunoassay (delfia), enhanced chemiluminescence and enzyme-linked immunosorbent assay (elisa). parameters which could affect the sensitivity and specificity of the assays were evaluated. different combinations of polyclonal antibody (pa) and five monoclonal antibodies (mabs) against the excretory/secretory antigens of the nema ... | 2001 | 11206114 |
| synthesis and antiparasitic activity of 2-(trifluoromethyl)-benzimidazole derivatives. | 2-(trifluoromethyl)benzimidazole derivatives substituted at the 1-, 5-, and 6-positions have been synthesized and in vitro tested against the protozoa giardia lamblia, entamnoeha histolytica. and the helminth trichinella spiralis. results indicate that all the compounds tested are more active as antiprotozoal agents than albendazole and metronidazole. one compound (20) was as active as albendazole against t. spiralis. these compounds were also tested for their effect on tubulin polymerization an ... | 2001 | 11206455 |
| outbreak of trichinellosis associated with arctic walruses in northern canada, 1999. | 2001 | 11236393 | |
| an outbreak of trichinellosis in farmed wild boar in finland. | nine farmed wild boar out of 25 slaughtered from a single farm were condemned at meat inspection because of trichinellosis. with rapd-pcr, trichinella spiralis was identified in all positive wild boar. out of the available serum samples (n=7), all wild boar which had failed the meat inspection showed seroconversion in elisa and western blotting, as did one additional animal which had passed the inspection. the animals became infected during an invasion of rats from an improperly closed dump near ... | 2000 | 11252814 |
| mast cell-independent impairment of host defense and muscle contraction in t. spiralis-infected w/w(v) mice. | in response to nematode infection, the host presumably attempts to create an unfavorable environment to prevent larval penetration of the host and to expedite parasite expulsion from the gut. in this study, we have used w/w(v) mice with or without mast cells after bone marrow reconstitution (bmr-w/w(v)) to examine the role of mast cells in the host response. w/w(v), bmr-w/w(v), and wild-type (+/+) mice were infected with trichinella spiralis. infected w/w(v) mice exhibited less tissue damage and ... | 2001 | 11254490 |
| down-regulation of inducible nitric-oxide synthase (nos-2) during parasite-induced gut inflammation: a path to identify a selective nos-2 inhibitor. | nitric oxide (no) possesses potent anti-inflammatory properties; however, an over-production of no will promote inflammation and induce cell and tissue dysfunction. thus, the ability to precisely regulate no production could prove beneficial in controlling damage. in this study, advantage was taken of the well characterized inflammatory response caused by an intestinal parasite, trichinella spiralis, to study the relationship between intestinal inflammation and the regulation of nitric oxide syn ... | 2001 | 11259640 |
| impaired protective immunity and t helper 2 responses in alymphoplasia (aly) mutant mice infected with trichinella spiralis. | the alymphoplasia (aly) mutation of mice prevents the development of systemic lymph nodes and peyer's patches. the mutant homozygotes (aly/aly) are partially deficient in both humoral and cell-mediated immune functions. in the present study, we show that adult worm expulsion was slightly delayed and that t helper 2 (th2)-type responses were partially defective in aly/aly mice after infection with trichinella spiralis. male aly/aly and aly/+ mice (8-weeks old) were infected with 400 muscle larvae ... | 2001 | 11260327 |
| the effects of trichinella spiralis infection on renal function in rats. | trichinella spiralis infection was induced in rats by oral feeding of infective larvae. four weeks later, renal function, including renal plasma flow (rpf), glomerular filtration rate (gfr), excretion rate of protein, sodium and potassium were determined using clearance technics. there were no significant changes in these parameters. however, plasma urea nitrogen was significantly higher in the infected group, suggesting that either an impaired regulation of renal tubular urea transport or an in ... | 2000 | 11289002 |
| oral inoculation with gymnorhynchus gigas induces anti-parasite anapyhylactic antibody production in both mice and rats and adverse reactions in challenge mice. | this study was performed to mimic human consumption of fish flesh infected with larvae of the fish cestode gymnorhynchis gigas and examine possible side effects thereof. both a rat and a mouse g. gigas oral inoculation model were used. the rat model was evaluated according to propensity to induce stress responses in three tissues and anaphylactic antibody production. the mouse model measured anti-g. gigas igg, m and a (h + l) levels in intestinal fluids, fecal suspensions and serum and specific ... | 2001 | 11294352 |
| a macrophage protein, ym1, transiently expressed during inflammation is a novel mammalian lectin. | oral infections of mice with trichinella spiralis induce activation of peritoneal exudate cells to transiently express and secrete a crystallizable protein ym1. purification of ym1 to homogeneity was achieved. it is a single chain polypeptide (45 kda) with a strong tendency to crystallize at its isoelectric point (pi 5.7). co-expression of ym1 with mac-1 and scavenger receptor pinpoints macrophages as its main producer. protein microsequencing data provide information required for full-length cd ... | 2001 | 11297523 |
| pcr-rflp patterns of four isolates of trichinella for rdna its1 region. | we have studied the genetic differences among four isolates of trichinella including a new strain of trichinella spiralis (iss 623) recently found from a human case who took a badger in korea. because they have a different host origin and came from geographically separated regions, we supposed the genetic pattern of the isolates might be different as had been previously reported. it was analysed by pcr-rflp analysis of the rdna repeat that can readily distinguish a species or strain from others. ... | 2001 | 11301589 |
| sylvatic and domestic trichinella spp. in wild boars; infectivity, muscle larvae distribution, and antibody response. | thirty-six wild boars were inoculated with trichinella spiralis, trichinella nativa, trichinella britovi, trichinella pseudospiralis (ussr), t. pseudospiralis (usa), t. pseudospiralis (aust), trichinella murrelli, trichinella t6, and trichinella nelsoni. the wild boars were killed at 5 and 10 wk postinoculation (pi), and the number of muscle larvae per g (lpg) of tissue was determined for 18 muscles or muscle groups. five weeks pi, all trichinella genotypes had established as muscle larvae, but ... | 2001 | 11318560 |
| [search for antibodies against trichinella spiralis in free roaming rodents caught in a zoological park from mexico city]. | a serological survey to search for antibodies against t. spiralis was performed in free roaming rats (n = 64) and mice (n = 35) caught in a zoological park from mexico city. serum samples were analyzed by elisa and immunoelectrotransfer blot assay (eibt). none serum show positive absorbance values in elisa nor recognized t. spiralis specific antigenic fractions in eibt. however, two rat samples recognized three antigens of 31, 37 y 55 kda, while one of them reacted with two additional antigens o ... | 2000 | 11338971 |
| secreted variant of nucleoside diphosphate kinase from the intracellular parasitic nematode trichinella spiralis. | the molecular components involved in the survival of the parasitic nematode trichinella spiralis in an intracellular environment are poorly characterized. here we demonstrate that infective larvae secrete a nucleoside diphosphate kinase when maintained in vitro. the secreted enzyme forms a phosphohistidine intermediate and shows broad specificity in that it readily accepts gamma-phosphate from both atp and gtp and donates it to all nucleoside and deoxynucleoside diphosphate acceptors tested. the ... | 2001 | 11349027 |
| trichinella spiralis-infected muscle cells: abundant rna polymerase ii in nuclear speckle domains colocalizes with nuclear antigens. | infection of mammalian skeletal muscle cells by trichinella spiralis causes host nuclei to become polyploid (ca. 4n) and abnormally enlarged. it has been postulated that this enlargement reflects an infection-induced elevation of host transcription. anthelmintic treatment of t. spiralis-infected rodents with mebendazole (mbz) causes a reduction in the size of infected cell nuclei and a significant reduction in the total rna content of individual infected muscle cells. a monoclonal antibody to th ... | 2001 | 11349077 |
| isolation of a new antibiotic oligomycin g produced by streptomyces sp. wk-6150. | 2001 | 11372788 | |
| role of immunologic factors and cyclooxygenase 2 in persistent postinfective enteric muscle dysfunction in mice. | chronic abdominal symptoms develop in some patients after acute enteric infection. this study examined mechanisms underlying smooth muscle hypercontractility that persists after acute infection in mice. | 2001 | 11375954 |
| il-12 gene transfer alters gut physiology and host immunity in nematode-infected mice. | immune responses elicited by nematode parasite infections are characterized by t helper 2 (th2) cell induction. the immunologic basis for changes in intestinal physiology accompanying nematode infection is poorly understood. this study examined whether worm expulsion and associated goblet cell hyperplasia and muscle contractility share a similar immune basis by shifting the response from th2 to th1 using interleukin-12 (il-12) overexpression. we used a single administration of recombinant adenov ... | 2001 | 11408260 |
| neural change in trichinella-infected mice is mhc ii independent and involves m-csf-derived macrophages. | intestinal inflammation due to nematode infection impairs enteric cholinergic nerve function and induces hypercontractility of intestinal muscle. macrophages have been implicated in the neural changes, but the subpopulation and mechanism involved are unknown. we examined whether macrophages alter nerves by virtue of their ability to activate lymphocytes via major histocompatibility complex (mhc) ii-restricted antigen presentation. we also attempted to evaluate the role of macrophage subsets usin ... | 2001 | 11408267 |
| hsp60, hsp70 and hsp90 from trichinella spiralis as targets of humoral immune response in rats. | this study identifies three heat shock proteins (hsps) using purified preparations from trichinella spiralis larvae. the proteins: hsp60, hsp70 and hsp90 were found to be targets of the humoral immune response in rats. three approaches were adopted to obtain t. spiralis hsp-enriched material and/or to purify hsps to homogeneity. the former product was prepared using affinity chromatography on gelatin sepharose and elution with atp. pure 90 kda-protein was isolated from parasite extract by sequen ... | 2001 | 11411944 |
| characterization of two monoclonal antibodies raised in btk(xid) mice that recognize phosphorylcholine-bearing antigens from trichinella and other helminths. | this study investigated the binding properties of two monoclonal antibodies (mabs us1 and us2) raised in (cba/n x balb/c)f1 (nbf1) btk(xid) male mice. both mabs show unusual specificity for phosphorylcholine (pc)-containing tsl4 antigens of trichinella. specifically, and in contrast to mabs raised in normal mice, us1 and us2 mabs do not bind to artificial pc-protein conjugates and are not inhibited by either free pc or nppc, although us2 was partially inhibited by nppc at high concentration (10( ... | 2001 | 11412384 |
| diallyl sulfide--a flavour component from garlic (allium sativum) attenuates lipid peroxidation in mice infected with trichinella spiralis. | we investigated the antioxidant capacity of diallyl sulfide (das) in the course of experimental trichinellosis in c3h mice. for this purpose, the mice were orally infected with either trichinella spiralis larvae (300 larvae/mouse) or treated per os with normal saline (control), and 12 hrs later, they were treated per os with das (200 mg/kg b.w) daily for 5 or 20 days. on day 5 and 35 of the post-infection period (5 and 35 dpi), thiobarbituric-acid reactive substances (tbars) in the small intesti ... | 2001 | 11417909 |
| secretion of the novel trichinella protein tsj5 by t. spiralis and t. pseudospiralis muscle larvae. | the trichinella tsj5 gene is preferentially expressed in muscle larvae of trichinella spiralis and encodes a novel protein. previous observations have shown tsj5 to be expressed at higher levels in encapsulating species than in non-encapsulating species and down-regulation of gene expression in t. pseudospiralis to be correlated with a lower protein abundance in the muscle larva of this species. in the present study we have determined the full-length cdna sequence of the tsj5 homologue in t. pse ... | 2001 | 11420106 |
| mcp-1 and rantes are mediators of acute and chronic inflammation. | regulation of leukocyte migration and activation by chemokines are recognized as potentially important functions in the induction of acute and chronic inflammatory reactions. regulated upon activation normal t cell expressed and presumably secreted (rantes), monocyte chemotactic protein-1 (mcp-1), and related molecules constitute the c-c class of the beta chemokine supergene family with inflammatory properties. here we report that in experimental studies rantes and mcp-1 provoke mast cell activa ... | 2001 | 11424873 |
| differential diagnosis of schistosomiasis mekongi and trichinellosis in human. | an indirect (plate) elisa and, a more convenient version, a dot-blot (membrane) elisa have been developed using haemocyanin of a mollusk, megathura crenulata, i.e. keyhole limpet haemocyanin (klh) and purified, specific antigen of trichinella spiralis (aptsag) obtained from a monoclonal antibody-affinity column chromatography, for differential diagnosis of schistosomiasis mekongi and trichinellosis. serum samples of patients with parasitologically confirmed trichinellosis were reactive to both a ... | 2000 | 11426576 |
| enhanced antigen-specific delayed-type hypersensitivity and immunoglobulin g2b responses after oral administration of viable lactobacillus casei yit9029 in wistar and brown norway rats. | in this study, the effects of orally administered viable lactobacillus casei shirota strain yit9029 on the immunity parameters of wistar and brown norway rats were examined. for this purpose, we used the trichinella spiralis host resistance model. two weeks before and during t. spiralis infection, rats were fed 10(9) viable l. casei bacteria 5 days per week. the t. spiralis-specific delayed-type hypersensitivity (dth) response was significantly enhanced in both wistar and brown norway rats given ... | 2001 | 11427423 |
| macrophage migration inhibitory factor of the parasitic nematode trichinella spiralis. | cdnas were obtained for macrophage migration-inhibitory factor (mif)/l-dopachrome methyl ester tautomerase homologues from the parasitic nematodes trichinella spiralis (tsmif) and trichuris trichiura (ttmif). the translated sequences, which were partly confirmed by sequencing of proteolytic fragments, show 42 and 44% identity respectively with human or mouse mif, and are shorter by one c-terminal residue. unlike vertebrate mif and mif homologues of filarial nematodes, neither tsmif nor ttmif con ... | 2001 | 11439086 |
| molecular cloning and characterization of a serine proteinase inhibitor from trichinella spiralis. | we produced a recombinant protein from a cdna library from muscle larvae of trichinella spiralis which had proteinase inhibitory activity. the predicted amino acid sequence of the clone had an identity of only 30% to the serine proteinase inhibitors (serpins) from caenorhabditis elegans or brugia malayi. at the putative reactive region, however, the identity was about 50%. the recombinant protein expressed in escherichia coli inhibited 82% of the activity of the serine proteinase (trypsin). stag ... | 2001 | 11467786 |
| different response of satellite cells in the kinetics of myogenic regulatory factors and ultrastructural pathology after trichinella spiralis and t. pseudospiralis infection. | infection of an intracellular parasitic nematode, trichinella spiralis, resulted in severe damage in muscle cells which was followed by activation and proliferation of satellite cells. the repairing process, shortly after the damage, histopathologically resembled those seen after mechanical injury. resemblance was also true for kinetics of expression of myogenic regulatory factors (myod, myogenin and mrf4). the difference resided in the next step where the muscle cell infected with t. spiralis t ... | 2001 | 11467787 |
| mucosal responses to infection with trichinella spiralis in mice. | infections with t. spiralis in mice elicit strong inflammatory responses. the nature and control of these responses, and their relationship to the process of worm expulsion, have been debated for many years. many components of inflammation are, like worm expulsion, t cell-dependent, but some are not. the paper describes novel observations on paneth cell responses to infection in immunologically normal mice and in a variety of t cell-deficient mice. responses occurred normally in nu/nu and scid/s ... | 2001 | 11484330 |
| interleukin mrna changes in mast cells stimulated by tsl-1 antigens. | in this work we analyzed by rt-pcr, the mrna changes for il-4, il-10, tnf and ifn (induced by tsl-1 antigens in a rat mast cell line (hrmc) with mucosal characteristics. the data obtained showed an increase of 65 and 52% in mrna expression for il-4 and tnf respectively and a decrease of 59 and 55% in mrnas for ifn gamma and il-10. our results suggest that tsl-1 antigens induce the release from mc of regulatory molecules, such as il-4 by an ige independent mechanism. our data also provides import ... | 2001 | 11484331 |