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endoparasites of stray dogs in mashhad, khorasan razavi province, northeast iran with special reference to zoonotic parasites.to find out different species of helminthes and blood/tissue protozoan parasites of stray dogs and their potential role for transmission of zoonotic species to human in mashhad, khorasan razavi province, northeast iran, during 2008-2009.201324454441
seroprevalence of parasitic zoonoses and their relationship with social factors among the canadian inuit in arctic regions.residents of arctic communities are at increased risk of contracting infectious diseases transmitted by wildlife. data collected from the international polar year inuit health survey were used to determine the seroprevalence of 4 parasitic zoonoses in three inuit jurisdictions of the canadian arctic and to assess risk factors of infection. to date, this is the most comprehensive survey of its kind. immunoenzymatic methods were used for the detection of antibodies against toxocara canis, echinoco ...201424461773
vertical transmission of toxocara canis in successive generations of mice.visceral toxocariasis is a neglected zoonosis caused by toxocara canis larvae in unusual hosts. in dogs, the definitive host, the infection occurs mainly through transplacental and transcolostral transmission. studies on experimental models have shown that vertical transmission may result from acute infections. considering that toxocariasis is characterized as a chronic infection, with possible reactivation of larvae present in the brain, this study evaluated the presence of larvae in the brain ...201424473895
prevalence of canine toxocariasis in bareilly, uttar pradesh, india.toxocara canis is one of the most common parasitic helminth worm of dogs and also a causative agent of zoonotic disease in humans. this pilot study was conducted to determine the presence of t. canis infection in dog population in and around bareilly, uttar pradesh, india. a total of 558 faecal samples both from stray and owned dogs were screened and overall 24.3 % dogs were found positive for t. canis. a comparison between owned and stray dogs suggests that the higher prevalence was observed in ...201424505188
anti-anisakis ige seroprevalence in the healthy croatian coastal population and associated risk factors.the main objective of the study was to determine the degree of sensitization to anisakis spp. antigens in healthy coastal population of dalmatia given the high thermally unprocessed fish intake rate present in this area, suggested as a significant risk factor for anisakiasis. we performed a monocenter, cross-sectional pilot study stratified by geographic area of residence, conducted at the county secondary healthcare provider medicine-biochemical laboratory in split (croatia), from november 2010 ...201424516680
endoparasites of the raccoon dog (nyctereutes procyonoides) and the red fox (vulpes vulpes) in denmark 2009-2012 - a comparative study.invasive species negatively influence the biodiversity of the ecosystems they invade and may introduce pathogens to native species. raccoon dogs have very successfully invaded europe, including, recently, denmark. this study included analyses of gastrointestinal helminths and trichinella spp. from 99 raccoon dogs and 384 native red foxes collected from october 2009 to march 2012. the sedimentation and counting method used revealed that raccoon dogs and foxes harboured 9 and 13 different helminth ...201324533328
environmental conditions predict helminth prevalence in red foxes in western australia.red foxes (vulpes vulpes) are the most common and widely distributed wild carnivore worldwide. these predators harbour a wide range of parasites, many of which may have important conservation, agricultural and zoonotic repercussions. this project investigated the occurrence of helminth parasites from the intestines of 147 red foxes across 14 sampling localities of southwest western australia. helminth parasites were detected in 58% of fox intestines: dipylidium caninum (27.7% of foxes), uncinari ...201324533331
evaluation of a rapid device for serological in-clinic diagnosis of canine angiostrongylosis.angiostrongylus vasorum is a potentially fatal canine nematode. due to the high variability of clinical signs and the often chronic and subtle course of the infections, the diagnosis is particularly challenging. a rapid in-clinic assay (angio detect test, idexx laboratories, westbrook, maine, usa) for the serological detection of circulating antigen and intended for routine in-clinic diagnosis has been evaluated.201424548539
infections with cardiopulmonary and intestinal helminths and sarcoptic mange in red foxes from two different localities in denmark.monitoring parasitic infections in the red fox is essential for obtaining baseline knowledge on the spread of diseases of veterinary and medical importance. in this study, screening for cardiopulmonary and intestinal helminths and sarcoptic mange (sarcoptes scabiei) was done on 118 foxes originating from two distinct localities in denmark, (copenhagen) greater area and southern jutland. fifteen parasite species were recorded in 116 foxes (98.3%), nine parasitic species are of zoonotic potential. ...201424570055
communicating hydrocephalus following eosinophilic meningitis is pathogenic for chronic viliuisk encephalomyelitis in northeastern siberia.viliuisk encephalomyelitis (ve) is an endemic neurological disease in northeast siberia and generally considered to be a chronic encephalomyelitis of unknown origin actually spreading in the sakha (yakutian) republic.201424586232
people, pets, and parasites: one health surveillance in southeastern saskatchewan.residents of remote and indigenous communities might experience higher exposure to some zoonotic parasites than the general north american population. human sero-surveillance conducted in two saulteaux communities found 113 volunteers exposed as follows: trichinella (2.7%), toxocara canis (4.4%), echinococcus (4.4%), and toxoplasma gondii (1.8%). in dogs, 41% of 51 fecal samples were positive for at least one intestinal parasite, 3% of 77 were sero-positive for borrelia burgdorferi, and 21% of 7 ...201424639298
[effects of disinfectants on toxocara canis eggs (results of experimental studies)].the authors investigated the effects of disinfectants, such as desavid (brand name desavid), delegol, dp-2t, sulfochloranthione, and hydrogen peroxide on toxocara canis eggs in the pure culture, desavid bas on them in the household-fecal sludge, and sodium hypochlorite in the soil cover. only desavid (brand name desavid), delegol, and sodium hypochlorite have a marked ovicidal effect.201424640128
[effects of household chemicals on infested toxocara canis eggs].six household chemicals were tested for effects on cultured toxocara canis eggs in the mobile larval stage. the cleansing gel comet (double effect) and its 10%, 25%, and 50% aqueous solutions have the most pronounced ovicidal and larvicidal effects under laboratory conditions (+19-22 degrees c). domestos and belizna (bleach) are also effective. these agents are recommended for the disinvasion and washing of premises for canids: aviaries of dog-breeding centers; farms for silver and polar foxes; ...201424640129
molecular characterization and functional analysis of serine/threonine protein phosphatase of toxocara canis.toxocara canis (t. canis) is a widely prevalent zoonotic parasite that infects a wide range of mammalian hosts, including humans. we generated the full-length complementary dna (cdna) of the serine/threonine phosphatase gene of t. canis (tc stp) using 5' rapid amplification of the cdna ends. the 1192-bp sequence contained a continuous 942-nucleotide open reading frame, encoding a 313-amino-acid polypeptide. the tc stp polypeptide shares a high level of amino-acid sequence identity with the predi ...201424657583
molecular characterization of toxocara spp. from soil of public areas in ahvaz southwestern iran.in the present study, the microscopy and polymerase chain reaction methods were used for detection and identification of soil contamination by toxocara eggs in squares, streets, public parks, and rubbish dumps in ahvaz, southwestern iran. a total of 210 soil samples were collected from different parts of the city and examined by microscopy and polymerase chain reaction (pcr) methods, following sodium nitrate flotation. nucleotide sequencing was performed to confirm the results of the pcr method. ...201424695242
the prevalence and diversity of intestinal parasitic infections in humans and domestic animals in a rural cambodian village.in cambodia, intestinal parasitic infections are prevalent in humans and particularly in children. yet, information on potentially zoonotic parasites in animal reservoir hosts is lacking. in may 2012, faecal samples from 218 humans, 94 dogs and 76 pigs were collected from 67 households in dong village, preah vihear province, cambodia. faecal samples were examined microscopically using sodium nitrate and zinc sulphate flotation methods, the baermann method, koga agar plate culture, formalin-ether ...201424704609
sequence variation in mitochondrial cox1 and nad1 genes of ascaridoid nematodes in cats and dogs from iran.the study was conducted to determine the sequence variation in two mitochondrial genes, namely cytochrome c oxidase 1 (pcox1) and nadh dehydrogenase 1 (pnad1) within and among isolates of toxocara cati, toxocara canis and toxascaris leonina. genomic dna was extracted from 32 isolates of t. cati, 9 isolates of t. canis and 19 isolates of t. leonina collected from cats and dogs in different geographical areas of iran. mitochondrial genes were amplified by polymerase chain reaction (pcr) and sequen ...201524717402
toxocariasis: seroprevalence in abandoned-institutionalized children and infants.toxocariasis is an infection that has worldwide distribution. toxocara canis is the most relevant agent due to its frequent occurrence in humans. soil contamination with embryonated eggs is the primary source of t. canis. this study aimed to determine the seroprevalence of toxocariasis in 10-month to 3 year-old abandoned infants, considered to be at high risk because of their orphanhood status and early age. blood samples were collected from 120 children institutionalized in an orphanage in the ...201424721267
behavioural changes and muscle strength in rattus norvegicus experimentally infected with toxocara cati and t. canis.toxocara canis and toxocara cati are nematode parasites in dogs and cats, respectively, transmitted by ingestion of embryonated eggs, transmammary and transplacental (t. canis) routes and paratenic host predation. many parasites use mechanisms that change the behaviour of their hosts to ensure continued transmission. several researchers have demonstrated behavioural changes in mouse models as paratenic hosts for t. canis. however, there have been no studies on behavioural changes in laboratory r ...201524725503
risk of infection by the consumption of liver of chickens inoculated with low doses of toxocara canis eggs.experimental studies and registries of cases of human toxocariasis have shown that the consumption of raw or undercooked offal of the paratenic host of toxocara canis may pose a risk of infection. thus, we evaluated the risk of infection due to the consumption of liver of chickens inoculated with different doses of embryonated t. canis eggs. doses were 5-100 times smaller than the ones previously employed in this type of study. groups of five chickens were inoculated with 5000 (control), 1000, 5 ...201424746238
a tick-borne segmented rna virus contains genome segments derived from unsegmented viral ancestors.although segmented and unsegmented rna viruses are commonplace, the evolutionary links between these two very different forms of genome organization are unclear. we report the discovery and characterization of a tick-borne virus--jingmen tick virus (jmtv)--that reveals an unexpected connection between segmented and unsegmented rna viruses. the jmtv genome comprises four segments, two of which are related to the nonstructural protein genes of the genus flavivirus (family flaviviridae), whereas th ...201424753611
neurotoxocarosis: marked preference of toxocara canis for the cerebrum and t. cati for the cerebellum in the paratenic model host mouse.infective larvae of the worldwide occurring zoonotic roundworm t. canis exhibit a marked affinity to the nervous tissues of paratenic hosts. in humans, most cases of neurotoxocarosis are considered to be caused by larvae of t. canis as t. cati larvae have rarely been found in the cns in previous studies. however, direct comparison of studies is difficult as larval migration depends on a variety of factors including mouse strains and inoculation doses. therefore, the present study aims to provide ...201424754900
a survey of intestinal helminths in wild carnivores from the tatra national park, southern poland.from january 2011 to july 2012, 144 faecal samples of wild carnivores from the tatra national park were examined to evaluate the prevalence of intestinal helminths--72 of wolves (canis lupus), 45 of red foxes (vulpes vulpes), 15 of pine martens (martes martes) and 12 of brown bears (ursus arctos). in wolves, monospecific infection with trichuris vulpis (13.9%), toxocara canis (6.9%), ancylostoma/uncinaria (5.6%) and taeniids (1.4%) was revealed. in red foxes, the most prevalent infection was 7 v ...201324791342
characterization of excretory-secretory antigens of adult toxocara canis by western blotting.toxocara canis is one of the most common helminth worm of dogs which continues to stimulate both public health concern alongside the higher scientific interest. it may cause visceral and ocular damage in humans especially in children. the identification of specific antigens of t. canis is important so as to develop better diagnostic techniques. excretory-secretory (es) antigens were prepared by culturing the adult t. canis worms in rpmi 1640 medium without serum supplementation followed by ammon ...201424808645
detection of toxocara eggs in contaminated soil from various public places of chennai city and detailed correlation with literature.toxocarosis is one of the most prevalent human helminthosis caused by larvae of toxocara canis and toxocara cati, the most widely distributed nematode parasites of dogs and cats respectively. soil is considered as the principal source of transmission of toxocara infection to human beings. with increasing population of dogs and cats, soil contamination with ova or eggs of toxocara can be detected in public and private locations of city backyards, playgrounds, streets, sand pits and so on, regardl ...201424808647
prevalence of toxoplasma gondii and toxocara canis among patients with uveitis.to investigate the prevalence of toxoplasma gondii and toxocara canis in patients with uveitis.201424833105
transmammary infection in balb/c mice with chronic toxocariasis.human toxocariasis is a neglected public health problem. infection of humans generally results from the accidental ingestion of embryonated toxocara canis eggs, but it is important to broaden knowledge about other forms of transmission. this study aimed to demonstrate the prevalence of transmammary transmission in mice with chronic toxocariasis. balb/c mice in groups 1 (g1) and 3 (g3) were inoculated with 1200 t. canis eggs 60days before mating, whereas those of group 2 (g2) were not infected. a ...201524837181
effects of high hydrostatic pressure processing on embryonation of toxocara canis eggs.toxocara canis is a zoonotic nematode parasite that can be transmitted to humans by food or water contaminated with t. canis eggs from infected dog feces. high-pressure processing (hpp) is a useful alternative to thermal treatments to eliminate pathogens from foods. most of the research on hpp has focused on prokaryotes, but little is known about its effects on eukaryotic organisms. we evaluated the ability of hpp to affect embryonation of t. canis eggs to test the hypothesis that hpp treatment ...201424866420
seroprevalence and modifiable risk factors for toxocara spp. in brazilian schoolchildren.toxocariasis is a worldwide helminthic zoonosis caused by infection with the larvae of the ascarid worms that comprise the toxocara spp. children are particularly prone to infection because they are exposed to the eggs in sandboxes and playgrounds contaminated with dog and cat feces. certain behaviors, such as a geophagy habit, poor personal hygiene, a lack of parental supervision, close contact with young dogs, and ingestion of raw meat, as well as gender, age, and socioeconomic status, affect ...201424874504
exposure to multiple parasites is associated with the prevalence of active convulsive epilepsy in sub-saharan africa.epilepsy is common in developing countries, and it is often associated with parasitic infections. we investigated the relationship between exposure to parasitic infections, particularly multiple infections and active convulsive epilepsy (ace), in five sites across sub-saharan africa.201424875312
effects of sub-zero storage temperatures on endoparasites in canine and equine feces.fecal samples from wild and domestic carnivores are routinely frozen for three days at -80°c to kill eggs of echinococcus spp., following recommendations from the world health organization (who) and world organization for animal health (oie). this is done to decrease the risk of zoonotic infection with these pathogenic cestodes. in addition, it is often necessary to freeze fecal samples collected for research prior to batch processing by a limited number of personnel, especially large numbers of ...201424880644
isolation of igg antibodies to toxocara in ankylosing spondylitis patients with acute anterior uveitis.since few reports had been published on the prevalence of toxocariasis in ankylosing spondylitis (as) patients with acute non-granulomatous anterior uveitis (angau), the aim of this work was to determine the presence of antibodies against toxocara canis in as patients with angau.201424882953
seroepidemiology of toxocara canis infection among primary schoolchildren in the capital area of the republic of the marshall islands.toxocariasis, which is predominantly caused by toxocara canis (t. canis) infection, is a common zoonotic parasitosis worldwide; however, the status of toxocariasis endemicity in the republic of the marshall islands (rmi) remains unknown.201424886153
unusual panuveitis in a child: toxocariasis associated with ocular myiasis.most panuveitis in children are caused by infectious agents. a detailed clinical history and clinical examination are helpful in the diagnosis, but specific techniques are sometimes required to identify the causing specimen. we report the first published case of panuveitis in a child caused by simultaneous ocular infection by toxocara canis and a fly larva and the innovative use of immunodiffusion technique in the vitreous for the diagnosis.201424916981
asthma and toxocariasis.asthma is the most common chronic disease in children and a major public health problem in adults. a relation between asthma and toxocariasis has been hypothesized.201424934109
intestinal helminths of golden jackals and red foxes from tunisia.forty wild canids including 31 golden jackals (canis aureus linné, 1758) and 9 red foxes (vulpes vulpes linné, 1758) collected between 2008 and 2011 in the northeast, northwest and center of tunisia were necropsied and examined for intestinal helminth parasites. all jackals and foxes were found infected with a prevalence rate of 95% for cestodes, 82.5% for nematodes and 7.5% for acanthocephalans. a total of twelve helminth species were recorded in red foxes: cestodes, dipylidium caninum (55.6%), ...201424938826
gastrointestinal parasites of dogs and cats in a refuge in nakhon nayok, thailand.we collected fecal samples from 500 dogs and 300 cats from an animal refuge in nakhon nayok province, thailand to test for gastrointestinal protozoa and helminths using a formalin-ether concentration technique. the overall prevalence of parasites in stool from dogs was 36.2% (181/500), 35.7% (177/500) had helminths and 2.8% (14/500) had protozoa. the helminths were: hookworm (30.6%), trichuris vulpis (16.0%), toxocara canis (6.6%), hymenolepis diminuta (1.2%), spirometra mansoni (0.6%), and dipy ...201424964651
[ocular toxocariasis: report of two cases and review of the literature]. 201425018821
[evaluation of the diagnostic value of the elisa tests developed by using eghf, em2 and emii/3-10 antigens in the serological diagnosis of alveolar echinococcosis].alveolar echinococcosis (ae), caused by larva stage of echinococcus multilocularis, is one of the lethal parasitic diseases of man and a major public health problem in many countries in the northern hemisphere. when the living conditions and habits in turkey were considered in terms of relation with the life cycle of the parasite, it was suggested that ae has been much more common than reported mainly from the eastern anatolia region of turkey. since in vitro serologic diagnosis tests with high ...201425052112
echinococcus granulosus and other intestinal helminths: current status of prevalence and management in rural dogs of eastern australia.ascertain the prevalence of intestinal helminths in rural dogs from eastern australia and tasmania. identify farm management practices contributing to the perpetuation and transmission of echinococcus granulosus.201425066196
behavioral and memory changes in mus musculus coinfected by toxocara canis and toxoplasma gondii.several researchers have stated that parasites can alter the behavior of their hosts, in order to increase the transmission rate, principally when prey-predator relationships are a reliable way of infection transmission. the aim of this study was to verify the occurrence of changes in anxiety and short-term memory patterns in experimentally infected mus musculus by toxocara canis and/or toxoplasma gondii. forty male mus musculus (balb/c) eight-week-old were divided into four groups of 10 mice ea ...201525076438
human toxocariasis: current advances in diagnostics, treatment, and interventions.toxocariasis is a neglected zoonosis caused by the nematodes toxocara canis and toxocara cati. this disease is widespread in many countries, reaching high prevalence independently of the economic conditions. however, the true number of cases of toxocariasis is likely to be underestimated owing to the lack of adequate surveillance programs. although some diagnostic tests are available, their sensitivity and specificity need to be improved. in addition, treatment options for toxocariasis are limit ...201425089038
mini-flotac, a new tool for copromicroscopic diagnosis of common intestinal nematodes in dogs.the most common intestinal nematodes of dogs are toxocara canis, hookworm and trichuris vulpis. the present study was aimed to validate a new copromicroscopic technique, the mini-flotac and to compare its diagnostic efficiency and sensitivity with four other copromicroscopic techniques: direct smear, tube flotation, wisconsin method and the flotac dual technique.201425095701
toxocara seroprevalence among clinically healthy individuals, pregnant women and psychiatric patients and associated risk factors in shandong province, eastern china.toxocarosis is a widespread zoonosis caused by the ascarid nematodes toxocara canis and toxocara cati, which primarily infect dogs and cats, respectively. most human infections with toxocara are asymptomatic; however, some infected individuals may develop a serious illness and even death. nevertheless, epidemiological knowledge regarding the prevalence and risks associated with toxocara infection is limited in china. therefore, we performed a cross-sectional pilot study and estimated the seropre ...201425101756
efficacy of milbemycin oxime in combination with spinosad in the treatment of larval and immature adult stages of ancylostoma caninum and toxocara canis in experimentally infected dogs.ancylostoma caninum and toxocara canis are two important zoonotic parasites of dogs. the primary objective of these studies were to confirm the oral effectiveness of milbemycin oxime (mo) and spinosad in dogs experimentally infected with immature (l4 and immature adult) stages of t. canis or a. caninum. both trials were conducted as randomized, blinded, placebo-controlled dose confirmation studies. treatments using the intended european commercial tablet formulation of trifexis were administered ...201425108851
visceral larva migrans syndrome: analysis of serum cytokine levels in children with hepatic lesions confirmed in radiological findings.human toxocariasis is a helminth zoonosis resulting from accidental infection of humans by the roundworms toxocara canis (t. canis) and cati (t. cati). the infection occurs in five forms: systemic (vlm), ocular, neurological, covert and asymptomatic. the aim of this study was to characterize the radiological and immunological findings in hepatic inflammation during the course of systemic infection by toxocara sp. in children. fifteen children, 2 to 17 years of age, with serological diagnosis of ...201425180780
proteinases in excretory-secretory products of toxocara canis second-stage larvae: zymography and modeling insights.components released in excretory-secretory products of toxocara canis larvae (tes) include phosphatidylethanolamine-binding proteins (tes26), mucins (tes120, muc2-5), and c-type lectins (tes32, tes70) and their biochemical, immunological, and diagnostic properties have been extensively studied albeit proteinase activities towards physiological substrates are almost unknown. proteolytic activities in tes samples were first analyzed by gel electrophoresis with gelatin as substrate. major activitie ...201425197645
in vitro culture of parascaris equorum larvae and initial investigation of parasite excretory-secretory products.currently, diagnosis of parascaris equorum infection in equids is limited to patent infections. the goals of this study were to culture p. equorum larvae in vitro and identify excretory-secretory (es) products for prepatent diagnostic testing. parascaris equorum l2/l3 larvae were hatched and cultured for up to 3 weeks for es product collection. fifth stage (l5) p. equorum were also cultured for es product collection. examination of es fractions by sodium dodecyl sulfate polyacrylamide gel electr ...201425209615
pulmonary toxocariasis mimicking invasive aspergillosis in a patient with ulcerative colitis.a 45-year-old-male who had underlying ulcerative colitis and presented with fever and dry cough. initially, the patient was considered to have invasive aspergillosis due to a positive galactomannan assay. he was treated with amphotericin b followed by voriconazole. nevertheless, the patient deteriorated clinically and radiographically. the lung biopsy revealed eosinophilic pneumonia, and elisa for toxocara antigen was positive, leading to a diagnosis of pulmonary toxocariasis. after a 10-day tre ...201425246722
determination of igg avidity in balb/c mice experimentally infected with toxocara canis.toxocariasis is a zoonotic disease in that igm titers can remain high for long periods making difficult to determine the stage of the disease. the aim of this study is to investigate the applicability of indirect elisa, associated with urea, to discriminate between the acute and chronic toxocariasis. igg avidity was evaluated in 25 balb/c mice experimentally infected with 1000 toxocara canis eggs. blood samples were collected, and sera treated with 6 m urea and assayed by elisa every two weeks. ...201625271464
comparison of the effect of the chosen species of saprotrophic fungi on the development of toxocara canis and ascaris suum eggs.the study aim was to compare the antagonistic interaction between saprotrophic soil fungi and embryonic development of geohelminths toxocara canis and ascaris suum. the experimental cultures were fertilized eggs of t.canis and a. suum incubated together with mycelium of strains: fusarium culmorum, metarhizium anisopliae,paecilomyces fumosoroseus, trichoderma viride and trichothecium roseum. in the control cultures the eggs of both nematode species were incubated without fungi. the experiment was ...201425281819
tissue-specific distribution of serine/threonine protein phosphatase 1 of toxocara canis.serine/threonine protein phosphatase 1 (pp1) is expressed in developing and reproductively active male toxocara canis. to investigate the tissue-specific expression of pp1 in t. canis, the pp1 protein was expressed in escherichia coli, and the recombinant protein was used to generate a rabbit polyclonal antiserum. indirect fluorescence immunohistochemical analysis of adult male t. canis showed that pp1 was expressed in the germ line tissues, primarily in the testis, seminal vesicle, vas deferens ...201425282049
research on toxocara canis antibodies obtained from patients with eosinophilia.eosinophils may suggest the presence of a great variety of anomalies whereupon allergies, malignancies, certain tissue disorders, idiopathic hypereosynophilic syndrome and parasitic infections (with the exception of protozoons) can be cited as a few. although the clinical manifestations may differ, the eosinophils level is quite an important data in cases related to the helminth infections. similarly, in parasitic infections related to larva migrans (visceral, cutaneous, ocular), the eosinophils ...201425297021
toxocara optic neuropathy after ingestion of raw meat products.to report toxocara optic neuropathy in five men with histories of ingestion of raw meat, liver, or blood.201425303840
study on gastrointestinal zoonotic parasites in pet dogs in western iran.dogs are the definitive or reservoirs hosts of more than 60 zoonotic parasites. this study was conducted to investigate the prevalence of gastrointestinal (gi) parasites in pet dogs in hamedan, western iran.201425308454
immunoblot for the detection of ascaris suum-specific antibodies in patients with visceral larva migrans (vlm) syndrome.visceral larva migrans (vlm) syndrome caused by toxocara canis larvae was first described in the 1950s. the role of other nematode larvae, i.e. the pig roundworm ascaris suum as a causative agent of visceral larva migrans-associated symptoms like general malaise, cough, liver dysfunction, hypereosinophilia with hepatomegaly and/or pneumonia, was discussed controversially during the last decades. recent serological screening studies for specific a. suum antibodies carried out in the netherlands a ...201525367210
adult ocular toxocariasis mimicking ciliary body malignancy.purpose. to discuss an unusual presentation of ocular toxocariasis. methods. case report. results. a 40-year-old woman presented with decreased vision in the left eye with a long history of recurrent red eye from uveitis. eosinophilia and positive elisa titers for toxocara canis favored the diagnosis of ocular toxocariasis. over 3 months, an anterior scleral mass had a rapid growth raising the possibility of medulloepithelioma, which rarely can mimic uveitic syndromes. surgical plan changed from ...201425371681
effects of helminth co-infections on atopy, asthma and cytokine production in children living in a poor urban area in latin america.helminths are modulators of the host immune system, and infections with these parasites have been associated with protection against allergies and autoimmune diseases. the human host is often infected with multiple helminth parasites and most studies to date have investigated the effects of helminths in the context of infections with single parasite or types of parasites (e.g. geohelminths). in this study, we investigated how co-infections with three nematodes affect markers of allergic inflamma ...201425410903
toxocara canis-associated visceral larva migrans of the liver. 201525462181
field evaluation of the efficacy and safety of a combination of spinosad and milbemycin oxime in the treatment and prevention of naturally acquired flea infestations and treatment of intestinal nematode infections in dogs in europe.two separate randomised, blinded, multicentre field trials were conducted to evaluate the efficacy and safety of a combination of spinosad and milbemycin oxime (mo) (trifexis(®), elanco animal health) in the treatment and prevention of naturally acquired flea infestations and intestinal nematode infections in european dogs. treatments using trifexis(®) and each control veterinary product (cvp) were administered once on day 0 in both field studies. in the flea field trial, 11 veterinary clinics i ...201525465736
toxocara canis in household dogs: prevalence, risk factors and owners' attitude towards deworming.the prevalence of gastrointestinal parasites and risk factors for shedding of toxocara eggs were determined for 916 dutch household dogs older than 6 months. additionally, the owners answered a questionnaire about their dogs and their attitude towards routine deworming was assessed. faecal samples were examined using the centrifugal sedimentation flotation method. the overall prevalence of dogs shedding toxocara eggs was 4.6 %. multivariable logistic regression analysis revealed that the risk fo ...201525468379
significance of serum antibody test for toxocariasis in healthy healthcare examinees with eosinophilia in seoul and gyeongsangnam-do, korea.there have been numerous reports on the relationship between eosinophilia and toxocariasis. the present study investigated seropositive rates of toxocariasis among healthy people with or without eosinophilia in urban and rural areas, and assessed risk factors for positive antibody test. a total of 610 healthy people, who visited health check-up (medicheck®, korea association of health promotion), 310 from seoul and 300 from gyeongsangnam-do, were subjected for this study. their serum samples wer ...201425469060
canine fecal contamination in a metropolitan area (milan, north-western italy): prevalence of intestinal parasites and evaluation of health risks.intestinal parasites of dogs represent a serious threat to human health due to their zoonotic potential. thus, metropolitan areas presenting high concentrations of pets and urban fecal contamination on public areas are at sanitary risk. major aim of this survey was to determine prevalence of zoonotic parasites in dog fecal samples collected from public soil of milan (north-western italy). differences in parasites prevalence distribution were explored by a geographical information system- (gis-) ...201425478583
the hamster (mesocricetus auratus) as an experimental model of toxocariasis: histopathological, immunohistochemical, and immunoelectron microscopic findings.toxocariasis is a globally distributed parasitic infection caused by the larval stage of toxocara spp. the typical natural hosts of the parasite are dogs and cats, but humans can be infected by the larval stage of the parasite after ingesting embryonated eggs in soil or from contaminated hands or fomites. the migrating larvae are not adapted to complete their life cycle within accidental or paratenic hosts like humans and laboratory animals, respectively, but they are capable of invading viscera ...201525518818
characterization of the ca2+-gated and voltage-dependent k+-channel slo-1 of nematodes and its interaction with emodepside.the cyclooctadepsipeptide emodepside and its parent compound pf1022a are broad-spectrum nematicidal drugs which are able to eliminate nematodes resistant to other anthelmintics. the mode of action of cyclooctadepsipeptides is only partially understood, but involves the latrophilin lat-1 receptor and the voltage- and calcium-activated potassium channel slo-1. genetic evidence suggests that emodepside exerts its anthelmintic activity predominantly through slo-1. indeed, slo-1 deficient caenorhabdi ...201425521608
prevalence of antibodies against selected zoonotic agents in forestry workers from eastern and southern poland.due to the nature of their work, foresters are exposed to many infectious pathogens from the wildlife reservoir. the primary aim of this study was to assess their contact with these pathogens.201425528917
cardiac involvement in hypereosinophilia associated with toxocariasis.cardiac involvement in hypereosinophilia is rare; when present, it manifests as ventricular thickening, usually with fibrous tissue and mural thrombosis. we present a case of a 57-year-old man with an abnormal right ventricular apex with eosinophilia, which was caused by toxocara canis infection.201425580199
differences in the clinical and radiological characteristics of lung-involved toxocariasis between toxocariasis with eosinophilia and those without eosinophilia.previously, most studies did not discuss about the characteristics of lung-involved toxocariasis without eosinophilia. however, the patients are not always accompanied by eosinophilia, so it is necessary to learn about the clinical and radiologic features that may predict pulmonary toxocariasis without eosinophilia. in addition, we also want to check the differences in characteristics between the two groups based on the presence of eosinophilia.201425589970
[potential hazard of zoonotic parasites present in canine feces in puerto escondido, oaxaca].to estimate the zoonotic parasites prevalence in feral dog feces in puerto escondido.201725604414
prevalence of intestinal parasites in breeding kennel dogs in japan.the present study is the first to show overall prevalences of intestinal parasites among breeding kennel dogs in japan. a total of 573 fresh fecal samples were collected from dogs at 12 breeding kennels. giardia-specific coproantigen was examined by elisa kit (snap(®) giardia, idexx laboratories, inc., maine, usa). other intestinal parasites were determined microscopically using the formalin-ethyl acetate sedimentation technique. overall prevalences of two genera of protists, giardia spp. and cy ...201525627028
genetic blueprint of the zoonotic pathogen toxocara canis.toxocara canis is a zoonotic parasite of major socioeconomic importance worldwide. in humans, this nematode causes disease (toxocariasis) mainly in the under-privileged communities in developed and developing countries. although relatively well studied from clinical and epidemiological perspectives, to date, there has been no global investigation of the molecular biology of this parasite. here we use next-generation sequencing to produce a draft genome and transcriptome of t. canis to support fu ...201525649139
seropositivity for ascariosis and toxocariosis and cytokine expression among the indigenous people in the venezuelan delta region.the present study aimed at measuring seropositivities for infection by ascaris suum and toxocara canis using the excretory/secretory (e/s) antigens from ascaris suum (aes) and toxocara canis (tes) within an indigenous population. in addition, quantification of cytokine expressions in peripheral blood cells was determined. a total of 50 warao indigenous were included; of which 43 were adults and seven children. in adults, 44.1% were seropositive for both parasites; whereas children had only serop ...201525651326
elevated trans-mammary transmission of toxocara canis larvae in balb/c mice.toxocariasis is a widespread zoonosis and is considered an important worldwide public health problem. the aim of this study was to investigate the frequency of trans-mammary toxocara canis infection in newborn balb/c mice nursed by females experimentally infected with 1,200 eggs after delivery. after 50 days of age, the presence of larvae in different organs of the offspring was investigated. trans-mammary infection was confirmed in 73.9% of the mice that had been nursed by infected females. the ...201725651332
copromicroscopic and molecular investigations on intestinal parasites in kenneled dogs.intestinal parasites are common in dogs worldwide, and their importance has recently increased for a renewed awareness on the public health relevance that some of them have. in this study, the prevalence of helminths and protozoa was evaluated by microscopy in 318 canine faecal samples collected from eight rescue shelters in the north-eastern italy; 285 of them were also submitted to the molecular characterization of giardia duodenalis and cryptosporidium spp. isolates. an analysis was performed ...201525687526
spatial distribution of canine zoonotic enteroparasites in bahía blanca, argentina.the objectives of this research were: (1) to determine the occurrence of zoonotic enteroparasites in dog feces from bahía blanca, argentina; (2) to characterize the spatial distribution of the parasites found in association with the quality of life index (qli) in neighborhoods of bahía blanca; and (3) to determine if the presence of a particular parasite genus in a stool sample was facilitated or impeded by the presence of other parasite genera. samples of dog stools (n=475) were collected betwe ...201525705047
differential serodiagnostics of toxocara canis and toxocara cati--is it possible?one of the most common zoonotic helminth infections is caused by species in the genus toxocara, particularly toxocara canis and t. cati (syn. t. mystax). however, their relative contribution to toxocarosis in humans remains largely unknown because causative larvae are seldom recovered and uncertainties regarding the validity of existing serological assays. in this study, we used sera from a pig model experimentally infected with t. canis and t. cati to evaluate whether a western blot could discr ...201525711956
[toxocara canis eggs as bait for soil fungus in a subtropical city].the use of different isolation techniques allows the recovery of fungi based on their ability to use selective substrates. the sprinkle method is a technique for the recovery of nematophagous fungi in the soil. these fungi are natural predators of nematodes and are widely distributed in nature.201525728877
toxocariasis diagnosed in international travelers at the institute of tropical medicine, antwerp, belgium, from 2000 to 2013.although infection with toxocara canis or t. catis (commonly referred as toxocariasis) appears to be highly prevalent in (sub)tropical countries, information on its frequency and presentation in returning travelers and migrants is scarce. in this study, we reviewed all cases of asymptomatic and symptomatic toxocariasis diagnosed during post-travel consultations at the reference travel clinic of the institute of tropical medicine, antwerp, belgium. toxocariasis was considered as highly probable i ...201525746418
immunopathological changes in the brain of immunosuppressed mice experimentally infected with toxocara canis.toxocariasis is a soil-transmitted helminthozoonosis due to infection of humans by larvae of toxocara canis. the disease could produce cognitive and behavioral disturbances especially in children. meanwhile, in our modern era, the incidence of immunosuppression has been progressively increasing due to increased incidence of malignancy as well as increased use of immunosuppressive agents. the present study aimed at comparing some of the pathological and immunological alterations in the brain of n ...201525748709
zoonotic parasites in feces and fur of stray and private dogs from italy.the present study evaluated the occurrence of zoonotic parasites in feces and on fur of stray and private dogs living in italy. individual fecal samples collected from 117 and 385 kenneled (i.e., rescue shelter) and privately owned dogs, respectively, were examined by conventional copromicroscopy. samples positive for giardia duodenalis and cryptosporidium spp. were subjected to further genetic characterization. additionally, 174 fur samples of private and kenneled dogs living in the same area, ...201525773179
neurotoxocarosis alters myelin protein gene transcription and expression.neurotoxocarosis is an infection of the central nervous system caused by migrating larvae of the common dog and cat roundworms (toxocara canis and toxocara cati), which are zoonotic agents. as these parasites are prevalent worldwide and neuropathological and molecular investigations on neurotoxocarosis are scare, this study aims to characterise nerve fibre demyelination associated with neurotoxocarosis on a molecular level. transcription of eight myelin-associated genes (cnp, mag, mbp, mog, mrf- ...201525773181
epidemiology of human toxocariasis in poland - a review of cases 1978-2009.toxocariasis is a helminthozoonosis due to the infection of humans with larvae belonging to the toxocara genus. humans become infected as a result of accidental consumption of infected eggs containing third stage larvae (l3) nematodes from toxocara canis or toxocara cati species. toxocariasis was recognized for the first time in the early 1950s, and the first cases of toxocariasis in poland were described a few years later. toxocariasis is clinically classified into several types: classic and in ...201525780823
vasculitis secondary to anti-c1q antibodies induced by toxocariasis.vasculitides occurring during parasitic infection are rare and may imply different mechanisms.201525808264
seroprevalence, disease awareness, and risk factors for toxocara canis infection among primary schoolchildren in makoko, an urban slum community in nigeria.in this study, we investigated the seroprevalence of toxocara canis infection in southern nigeria, which previously was unknown, in addition to evaluating disease awareness and potential risk factors for schoolchildren in an urban slum community. in total, 366 primary schoolchildren were investigated for the presence of anti-toxocara igg antibodies. blood was collected and screened by a western blot analysis based on the excretory-secretory antigens of larval t. canis (tces), targeting low molec ...201525812836
wildlife reservoirs for vector-borne canine, feline and zoonotic infections in austria.austria's mammalian wildlife comprises a large variety of species, acting and interacting in different ways as reservoir and intermediate and definitive hosts for different pathogens that can be transmitted to pets and/or humans. foxes and other wild canids are responsible for maintaining zoonotic agents, e.g. echinococcus multilocularis, as well as pet-relevant pathogens, e.g. hepatozoon canis. together with the canids, and less commonly felids, rodents play a major role as intermediate and par ...201425830102
microarray gene expression analysis reveals major differences between toxocara canis and toxocara cati neurotoxocarosis and involvement of t. canis in lipid biosynthetic processes.toxocara canis and toxocara cati are globally occurring intestinal nematodes of dogs and cats with a high zoonotic potential. migrating larvae in the cns of paratenic hosts, including humans, may cause neurotoxocarosis resulting in a variety of neurological symptoms. toxocara canis exhibits a stronger affinity to the cns than t. cati, causing more severe neurological symptoms in the mouse model. pathomechanisms of neurotoxocarosis as well as host responses towards the respective parasite are mos ...201525843806
toxocariasis: an unusual cause of pleural effusion.toxocara canis, one of the most frequent parasites worldwide, rarely triggers respiratory symptoms. we report the case of a 5-year-old girl hospitalized for a unilateral eosinophilic pleural effusion due to toxocara canis. besides the fact that she was living in a squat, no other medical condition was reported. there was no other site of infection caused by the parasite and she was successfully treated with albendazole. this case report is obviously unique as very few cases of pleural effusion d ...201525847394
enhanced resolution of eosinophilic liver abscess associated with toxocariasis by albendazole treatment.visceral larva migrans, caused by toxocara canis and toxocara cati, has emerged as a significant cause of eosinophilic liver abscess (ela). differentiation of ela associated with toxocariasis (ela-t) from metastasis or primary liver malignancy is sometimes difficult. however, the role of albendazole treatment remains uncertain in this condition. the aim of this study was to evaluate whether albendazole can enhance the radiologic resolution of ela-t.201525896156
field evaluation of a rapid diagnostic test to detect antibodies in human toxocariasis.human toxocariasis which is caused mainly by the larvae of toxocara canis and toxocara cati, is a worldwide zoonotic disease that can be a potentially serious human infection. the enzyme-linked immunosorbent assay (elisa) using t. canis excretory-secretory (tes) antigens harvested from t. canis larvae is currently the serological test for confirming toxocariasis. an alternative to producing large amounts of toxocara tes and improved diagnosis for toxocariasis is through the development of highly ...201525910623
[role of helminth antigens in the abnormal mitosis of bone marrow cells in laboratory animals].the intraabdominal administration of somatic extracts of the cestodes hydatigera taeniaformis batsch 1786, lamarck, 1816 and diphyllobothrium latum linnaeus, 1758 and the nematodes anisakis simplex larva rudolphi 1809, toxocara canis railliet et henry, 1912 in albino mice proved that these helminths had a karyopathic effect on the bone marrow cells of the animals. the antigenic composition of these extracts was investigated using the agar gel immunodiffusion test. the antigenic composition of th ...201525924282
[disinvasive activity of briz 25% e.c. against toxocara canis eggs (experimental studies)].the insectoacaricid briz 25% e.c. was tested for its effects on toxocara canis eggs in pure culture. in the solar-illuminated laboratory at 22-23°c, t. canis eggs of pure culture were destroyed on days 17-18 when in 1.25% aqueous emulsion of the insectoacaricid briz 25% e.c. (0.313% with reference to the active ingredient cypermethrin). within the egg, the parasite failed to die not only in the stage of protoblast (5-6 days), but also in the stages of semiformed (4 days) and mobile larva (7-8 da ...201525924285
cross-reactivity of toxocariasis with crude antigen of toxascaris leonina larvae by elisa.roundworms of toxocara canis and toxascaris leonina are common gastrointestinal helminths of canids over the world. humans are infected with t. canis larvae through ingestion of infective eggs in contaminated environments or larvae by consumption of raw or uncooked meat or livers. recently, patients of clinically diagnosed toxocariasis are increasing and require correct diagnosis in korea. the present study investigated serological cross-reactivity between crude antigens of t. canis (tcla) and t ...201525931784
successful capture of toxocara canis larva antigens from human serum samples.toxocara canis is a nematode that parasitizes dogs, while humans are paratenic hosts. when humans are infected the migrating larvae damage the liver, lungs and even the nervous system. larva migrans diagnosis is based on immunological techniques; however, the commercial immunodiagnostic kits detect anti-t. canis antibodies which may cross-react with other parasites, mainly nematodes with extra-intestinal migration. moreover, antibodies do not necessarily reflect an active infection; so detection ...201525952316
intestinal parasites of children and adults in a remote aboriginal community of the northern territory, australia, 1994-1996.parasitic infections can adversely impact health, nutritional status and educational attainment. this study investigated hookworm and other intestinal parasites in an aboriginal community in australia from 1994 to 1996.201725960921
parasite prevalence in fecal samples from shelter dogs and cats across the canadian provinces.in canada, surveys of enteric parasites in dogs and cats have been reported sporadically over the past 40 years, mostly focusing on a specific region. the present work was performed to determine the current prevalence of various parasites in fecal samples from shelter dogs and cats across the canadian provinces.201526013283
presence of anti-toxocara canis antibodies and risk factors in children from the amecameca and chalco regions of méxico.toxocariasis is a zoonotic disease that poses a threat to public health worldwide. this disease primarily affects children and is caused by the presence in the digestive tract of a common roundworm of dogs, toxocara canis, or cats, toxocara cati. toxocara is responsible for the presentation of various syndromes in humans depending on the affected organs.201526024905
molecular mechanism of serine/threonine protein phosphatase 1 (pp1cα-pp1r7) in spermatogenesis of toxocara canis.toxocariasis is one of the most important, but neglected, zoonoses, which is mainly caused by toxocara canis. to better understand the role of serine/threonine protein phosphatase 1 (pp1) in reproductive processes of male adult t. canis, differential expression analysis was used to reveal the profiles of pp1 catalytic subunit α (pp1cα) gene tc-stp-1 and pp1 regulatory subunit 7 (pp1r7) gene tcm-1309. indirect fluorescence immunocytochemistry was carried out to determine the subcellular distribut ...201526026715
fungi predatory activity on embryonated toxocara canis eggs inoculated in domestic chickens (gallus gallus domesticus) and destruction of second stage larvae.the objective of this study was to evaluate the infectivity of toxocara canis eggs after interacting with isolated nematophagous fungi of the species duddingtonia flagrans (ac001) and pochonia chlamydosporia (vc4), and test the predatory activity of the isolated ac001 on t. canis second stage larvae after 7 days of interaction. in assay a, 5000 embryonated t. canis eggs previously in contact with the ac001 and vc4 isolated for 10 days were inoculated into domestic chickens (gallus gallus domesti ...201526032943
production of toxocara cati tes-120 recombinant antigen and comparison with its t. canis homolog for serodiagnosis of toxocariasis.toxocariasis is a cosmopolitan zoonotic disease caused by the infective larvae of toxocara canis and t. cati. diagnosis in humans is usually based on clinical symptoms and serology. immunoglobulin g (igg)-enzyme-linked immunosorbent assay kits using t. canis excretory-secretory (tes) larval antigens are commonly used for serodiagnosis. differences in the antigens of the two toxocara species may influence the diagnostic sensitivity of the test. in this study, t. cati recombinant tes-120 (rtes-120 ...201526033026
toxocara canis mucins among other excretory-secretory antigens induce in vitro secretion of cytokines by mouse splenocytes.the effect of toxocara larval antigens on cytokine secretion by mouse splenocytes was studied in vitro. recombinant mucins were produced in pichia pastoris yeast, and toxocara excretory-secretory (tes) antigens were collected from in vitro culture of l2 larvae. tc-muc-2, tc-muc-3, tc-muc-4, and tc-muc-5 were expressed as glycoproteins and were specifically recognized by toxocara canis-infected dog serum antibodies. mouse splenocytes stimulated with recombinant mucins produced il-5, il-6, and tgf ...201526044883
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