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microbial composition of supra- and subgingival plaque in subjects with adult periodontitis.the purpose of the present study was to compare and relate the microbial composition of supra and subgingival plaque in 23 adult periodontitis subjects (mean age 51 +/- 14 years).200011034118
automated immunofluorescence for enumeration of selected taxa in supragingival dental plaque.the present study investigated a recently developed automated image analysis technique for its applicability to the enumeration of selected bacteria in supragingival dental plaque. following initial calibration, the system is capable to count fluorescence-labeled target cells in up to 48 samples without user interference. test samples contained a characteristic mixture of planktonic bacteria, small almost planar bacterial aggregates, and large, virtually indisruptable clumps with cells from mult ...200011037755
competition for glucose between candida albicans and oral bacteria grown in mixed culture in a chemostat.the competition for glucose as a growth-limiting substrate between candida albicans and a mixed community of oral bacteria was investigated. a chemostat was operated under glucose-limiting and glucose excess conditions at a dilution rate of 0.05/h. a mixed population of oral bacteria was established and after a steady state had been reached the chemostat was inoculated with c. albicans. seven bacterial species streptococcus sanguis, s. sobrinus, s. mitis, lactobacillus casei, veillonella dispar, ...200011073150
fever and pain over the right hypochondrium. liver abscess as the presenting form of stromal intestinal tumor. 200011076187
sensitization of actinomyces naeslundii and streptococcus sanguis in biofilms and suspensions to acid damage by fluoride and other weak acids.fluoride and other weak acids, such as benzoate, indomethacin, salicylate and sorbate, were found to be sensitizers for acid killing of cells of actinomyces naeslundii atcc 19246 and streptococcus sanguis nctc 10904 in suspensions or in mono-organism biofilms on glass slides. these bacteria are among the more acid-sensitive organisms from dental plaque and were killed when acidified to ph values between 3.5 and 4.0. biofilm cells were more resistant than cells in suspensions, especially in terms ...200011081793
fractional inhibitory concentration index of combinations of antibacterial agents against cariogenic organisms.to examine the inhibitory effects between paired combinations of four antibacterial agents that have been investigated in dentistry against six bacteria associated with dentinal caries.200011082524
images in cardiology: silent patent ductus arteriosus endarteritis. 200011083739
[general syndrome and fever]. 200011083959
similarity of bacteriocin activity profiles of mutans streptococci within the family when the children acquire the strains after the age of 5.it has been shown that there is a window of infectivity for mutans streptococci between the ages of 19 and 31 months, when many children acquire mutans streptococci transmitted from their mothers. part of the children that escape this window acquire mutans streptococci at a later age. in this group, maternal transmission is expected to be less prevalent. the present study compared the bacteriocin activity profiles of mutans streptococci isolated from mothers, fathers and children when the childr ...200011093022
strain-related acid production by oral streptococci.acid production, in particular at low ph, is thought to be an important ecological determinant in dental caries. the aim of the present study was to determine the acid producing capability at different ph levels of 47 streptococcal strains, representing 9 species, isolated from human dental plaque. the bacteria were grown until mid log-phase under anaerobic conditions and acid production was measured in a ph-stat system at ph 7.0, 6.0, 5.5 and 5.0. at all ph values, the mean velocity of acid pro ...200011093023
aneurysm of the anterior leaflet of the mitral valve secondary to aortic valve endocarditis.aneurysm of the anterior mitral leaflet is a rare complication of infective aortic valve endocarditis, the natural evolution of which is generally its rupture, with subsequent acute and severe mitral regurgitation. its presence cannot be recognized with transthoracic echocardiography and even in surgery. we describe a 78-year-old man with aortic valve endocarditis, in whom transesophageal echocardiography was essential for the diagnosis of this complication, its therapeutic management, and the p ...200011093109
dental plaque formation.dental plaque is a complex biofilm that accumulates on the hard tissues (teeth) in the oral cavity. although over 500 bacterial species comprise plaque, colonization follows a regimented pattern with adhesion of initial colonizers to the enamel salivary pellicle followed by secondary colonization through interbacterial adhesion. a variety of adhesins and molecular interactions underlie these adhesive interactions and contribute to plaque development and ultimately to diseases such as caries and ...200011113379
properties of streptococcus sanguinis glucans formed under various conditions.the aim of our study was to determine whether the structure of glucans formed by glucosyltransferase from streptococcus sanguinis (gtfss) on a surface differ from those formed in solution and to explore the effects of antiserum to gtfs, control normal rabbit serum, starch hydrolysates (sth) and dextran on s. sanguinis (gtfss) glucan. linkage analyses showed that solution-formed glucans are predominantly alpha-1,6-linked and have a small amount of alpha-1,3-linked glucose. surface-formed glucans ...200111125200
diversity and contribution of the intestinal bacterial community to the development of musca domestica (diptera: muscidae) larvae.the bacterial diversity in the intestinal tract of musca domestica l. was examined in larvae collected from turkey bedding and corn silage. aerobic culturing yielded 25 bacterial species, including 11 from larvae collected from turkey bedding and 14 from larvae collected from corn silage. providencia rettgeri (hadley, elkins & caldwell) was the only species common to both environments. two mammalian pathogens, yersinia pseudotuberculosis (pfeiffer) and ochrobactrum anthropi (holmes), were isolat ...200011126551
postantibiotic effects and postantibiotic sub-mic effects of amoxicillin on streptococcus gordonii and streptococcus sanguis.amoxicillin is one of the most frequently recommended antibiotics for prophylaxis of infective endocarditis in dental/oral procedures. in this study, the postantibiotic effect (pae), postantibiotic sub-mic (pasme) and sub-mic effect (sme) of amoxicillin on oral streptococci, streptococcus gordonii and streptococcus sanguis, which are two of the major etiological agents in infective endocarditis, were investigated. the pae was induced by 10 x mic of amoxicillin for 2 h and the antibiotic was elim ...200011128556
the galu gene of streptococcus pneumoniae that codes for a udp-glucose pyrophosphorylase is highly polymorphic and suitable for molecular typing and phylogenetic studies.the enzyme utp-glucose-1-phosphate uridylyltransferase (udp-glucose pyrophosphorylase, udpg:pp) is synthesized by practically all organisms, although prokaryotic udpg:pps are evolutionarily unrelated to the eukaryotic counterparts. the primary structure of prokaryotic udpg:pps is well conserved, although little information exists on the polymorphism of the genes coding for these enzymes. it has been reported that the galu gene encoding the streptococcus pneumoniae udpg:pp is absolutely required ...200011137293
[bact-alert system for hemocultures: evaluation of terminal subcultures].few laboratory microbiological procedures are as important as the isolation of microorganisms from blood. to evaluate the usefulness of the terminal subcultures, 5669 blood cultures giving negative results after 7 days of incubation in the bact/alert system (organon teknika) were studied. bottles were distributed as follows: 1562 adult aerobic bottles, 119 adult anaerobic bottles, 3960 pediatric bottles and 28 fan bottles. from 5669 blood cultures, 10 subcultures that yielded growth had not been ...200011149151
the oral microbiota of children undergoing liver transplantation.this study investigated the oral microbiota of children undergoing liver transplantation. oral swabs were taken using a standardized procedure from 27 children before liver transplantation and at 3 and 100 days post-transplantation and from 27 healthy controls at baseline and 90 days. viridans streptococci, yeasts, staphylococci, enterococci and enterobacteriaceae were enumerated and identified using conventional techniques. the oral microbiota of the patients changed significantly immediately p ...200011154404
porphyromonas gingivalis platelet aggregation activity: outer membrane vesicles are potent activators of murine platelets.recent evidence has established an association between chronic periodontitis and cardiovascular disease. periodontitis is a chronic inflammatory disease caused by a small group of gram-negative bacteria, of which porphyromonas gingivalis is considered an important causative agent. it has been proposed that dental plaque bacteria and their products can disseminate into the bloodstream from the site of infection and promote thromboembolic events associated with atherosclerosis and myocardial infar ...200011154438
profile of subgingival microbiota in children with mixed dentition.a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ...200011155173
the clinical and microbiological effects of systemic ornidazole in sites with and without subgingival debridement in early-onset periodontitis patients.the purpose of this study was to evaluate the clinical and microbiological effects of systemic ornidazole (orn) in sites with or without subgingival debridement in early-onset periodontitis (eop) patients.200011156043
commercial preparations of lipoteichoic acid contain endotoxin that contributes to activation of mouse macrophages in vitro.lipoteichoic acids (lta), cell wall components of gram-positive bacteria, have been reported to induce various inflammatory mediators and to play a key role in gram-positive-microbe-mediated septic shock. in a large number of these studies, investigators used commercially available lta purified from a variety of gram-positive bacteria, including staphylococcus aureus, bacillus subtilis, and streptococcus sanguis. we report here that, although these commercially available lta could be readily sho ...200111159964
selection of oral microbial adhesion antagonists using biotinylated streptococcus sanguis and a human mixed oral microflora.a microtitre assay has been developed using hydroxyapatite-coated wells and streptococcus sanguis nctc 10904 at 10(7) cells per ml. a number of models representing toothpaste and mouthwash usage were adopted to detect the anti-adherent efficacy of a polyvinylmethylether maleic acid copolymer (pvm/ma), polyoxypropylene/polyoxyethylene block copolymer (po/eo), two casein-derived peptides and selected silicones. the results not only confirmed the anti-adherence property of the selected components b ...200111163320
post-ph effect in oral streptococci.to study the post-ph effect (pphe) in 11 strains of oral streptococci belonging to streptococcus sanguis, s. mitis, s. gordonii, s. mutans and s. sobrinus by using the bact/alert microbial detection system.200011168090
utilization and acid production of beta-galactosyllactose by oral streptococci and human dental plaque.beta-galactosyllactose is a trisaccharide containing the beta-galactosidic linkage at the nonreducing end. the purpose of this study was to determine whether certain oral streptococci could utilize four kinds of beta-galactosyllactoses. three of four beta-galactosyllactoses were unable to support growth of the oral streptococci and to be a substrate for producing acid from the cell suspensions and dental plaque. 4'-beta-galactosyllactose supported growth of streptococcus sanguis atcc 35105, atcc ...200111169141
[the effect of chlorhexidine varnish system on streptococcus mutans in fissure plaques].a chlorhexidine varnish system and a controlled releasing varnish were prepared by the authors. the release rate of chlorhexidine in vitro and the effects on s. mutans and s. sanguis in vivo were determined. the results showed that the system could selectively inhibit the growth of s. mutans in fissure plaque and had no effect on s. sanguis. the period of interaction between the layer of varnish and plaque was prolonged by the control-releasing reagent. the study suggests that the chlorhexidine ...199711189283
[effects of sodium doecyl sulfate on the artificial dental plaque in chemostat].sodium doecyl sulfate (sds) is widely used as a detergent in dentifrices. it has been shown to interfere with the protein adsorption to hydroxyapatite (ha), and inhibit acquired pellicle formation. the aim of the present study was to examine the effects of sds on the artificial dental plaque in chemostat. the amount of the 3h-labelled bacteria adhered on the enamel fragment surface was determined with scintillometer. the artificial dental plaque was observed under the scanning electron microscop ...199711189307
in vitro study of the penetration of streptococcus sanguis and prevotella intermedia strains into human dentinal tubules.the persistent presence of bacteria in the root canal system often leads to the failure of treatment. the purpose of this study was to determine in vitro penetration of streptococcus sanguis and prevotella intermedia into dentinal tubules. samples obtained from human teeth were inoculated with a strain of s. sanguis (nctc 7853) and p. intermedia (nctc 93336) for 20 days. bacterial penetration into tubules was investigated at scanning electron microscopy and light microscopic level. the results s ...200011199726
comparative evaluation of antibacterial effects of nd:yag laser irradiation in root canals and dentinal tubules.the antibacterial effects of the nd:yag laser on contaminated root canals and dentinal tubules were observed as the aim of this study. the samples were inoculated with streptococcus sanguis (nctc 7853) and prevotella intermedia (nctc 93336), and the effects of nd:yag laser were tested on these teeth. the specimens were lased with 1.8 w and 2.4 w nd:yag laser for 30 s, and the presence of bacteria in tubules was observed under light microscopy. the 1.8 w laser sterilized the tubules in 86.3% of s ...200011199732
addition of antibacterial agents to mma-tbb dentin bonding systems--influence on tensile bond strength and antibacterial effect.to produce a bonding system which has both high bond strength and antibacterial properties, an antibacterial agent (vancomycin: vcm or metronidazol: mn) was added to the pmma powder of 4-meta/mma-tbb resin (cb). the influence of the addition of an antibacterial agent on tensile bond strength to dentin and the antibacterial effect were investigated in this study. forty-seven freshly extracted bovine first or second incisors were used to measure the tensile bond strength to dentin. the bond streng ...200011219091
mannosidase production by viridans group streptococci.the production of mannosidase activity by all currently recognized species of human viridans group streptococci was determined using an assay in which bacterial growth was dependent on the degradation of the high-mannose-type glycans of rnase b and subsequent utilization of released mannose. rnase b is an excellent substrate for the demonstration of mannosidase activity since it is a glycoprotein with a single glycosylation site which is occupied by high-mannose-type glycoforms containing five t ...200111230417
binding of oral streptococci to human fibrinogen.the interaction of oral streptococci with human fibrinogen was investigated. streptococcus gordonii was chosen as a representative species to study the binding to fibrinogen. s. gordonii dl1 adhered to immobilized fibrinogen and bovine serum albumin. binding to immobilized fibrinogen was saturable, concentration and temperature dependent. the binding of s. gordonii dl1 to fibrinogen was inhibited by anti-fibrinogen antibody. heating of the bacteria for 1 h at 95 degrees c resulted in 90% inhibit ...200111240861
ph-regulated secretion of a glyceraldehyde-3-phosphate dehydrogenase from streptococcus gordonii fss2: purification, characterization, and cloning of the gene encoding this enzyme.streptococcus gordonii and other viridans streptococci (vs) are primary etiologic agents of infective endocarditis, despite being part of the normal oral microflora. recently, a surface-bound glyceraldehyde-3-phosphate dehydrogenase (gapdh) has been found on the cells of all tested streptococcal species, where it has been implicated as a virulence factor. in contrast, we observed that a soluble extracellular gapdh was the major secreted protein from s. gordonii fss2, an endocarditis strain. the ...200111269731
oral colonization and cariogenicity of streptococcus gordonii in specific pathogen-free tan:spfom(om)br rats consuming starch or sucrose diets.the significance of streptococcus gordonii in dental caries is undefined, as is that of other alpha-amylase-binding bacteria (abb) commonly found in the mouth. to clarify the ecological and cariological roles of s. gordonii our specific pathogen-free osborne-mendel rats, tan:spfom(om)br, were fed either diet 2000 (containing 56% confectioner's sugar, most of which is sucrose) or diet 2000cs (containing 56% cornstarch, in lieu of confectioner's sugar) and inoculated with s. gordonii strains. unin ...200111269866
monocytes augment bacterial species- and strain-dependent induction of tissue factor activity in bacterium-infected human vascular endothelial cells.in bacterial endocarditis (be), intravascular infection with staphylococcus aureus, streptococcus sanguis, or staphylococcus epidermidis can lead to formation of a fibrin clot on the inner surface of the heart and cause heart dysfunction. the events that start the coagulation in the early stage of the disease are largely unknown. we have recently shown that human endothelial cells (ec) upon binding and internalization of s. aureus, but not s. sanguis or s. epidermidis, express tissue factor (tf) ...200111292691
interleukin-10 regulates the tissue factor activity of monocytes in an in vitro model of bacterial endocarditis.monocytes are important effector cells in the pathogenesis of bacterial endocarditis since they provide the tissue factor that activates the coagulation system and maintains established vegetations. monocytes secrete cytokines that can modulate monocyte tissue factor activity (tfa), thereby affecting the formation and maintenance of vegetations. in this study, we show that monocytes cultured for 4 h on a streptococcus sanguis-infected fibrin matrix mimicking the in vivo vegetational surface expr ...200111292741
investigation on interaction between streptococcus sanguis and porphyromonas gingivalis in specific pathogen-free rats.to examine whether endogenous streptococcus can prevent or reduce the colonization of the virulent porphyromonas gingivalis strain.200011314089
effects of three different infant dentifrices on biofilms and oral microorganisms.the purpose of this work was to evaluate the effects of infant dentifrices: a--with lactoperoxidase, glucose oxidase and lactoferrin; b--with 1100 ppm of naf and sodium lauryl sulfate; c--with extract of calendula. the dentifrices were test on biofilms formed in vitro from saliva and dental plaque of infants, using reference strains a. viscosus (atcc 43146); c. albicans (atcc 51501); l. casei (atcc 4646); s. mitis (atcc 49456); s. mutans (atcc 25175); s. oralis (atcc 35037); s. sanguis (atcc 105 ...200011314149
recommended conservation of the names streptococcus sanguis, streptococcus rattus, streptococcus cricetus, and seven other names included in the approved lists of bacterial names. request for an opinion.with reference to the first principle of the international code of nomenclature of bacteria, which emphasizes stability of names, it is proposed that the original names streptococcus sanguis, streptococcus rattus, streptococcus cricetus, erwinia ananas, eubacterium tarantellus, lactobacillus sake, nitrosococcus oceanus, pseudomonas betle, rickettsia canada and streptomyces rangoon, all included in the approved lists of bacterial names, be conserved. request for an opinion.200111321121
quinolone-resistance mechanisms and in vitro susceptibility patterns among european isolates of streptococcus mitis, streptococcus sanguis, and streptococcus pneumoniae. 200111347679
a case-control study of respiratory disease in thoroughbred racehorses in sydney, australia.in order to investigate the role of infectious agents in the aetiology of lower respiratory tract disease in thoroughbred racehorses, a matched case-control study was conducted. cases were identified by the presence of coughing, and were compared to a control population matched on time of sample collection and location within the same training establishment. tracheal wash samples were collected from 100 cases and 148 controls. case horses were more likely than controls to have endoscopic and cyt ...200111352347
are dental infections a cause of brain abscess? case report and review of the literature.dental pathology and/or treatment have been linked to a small number of brain abscesses as possible sources of infection. a further case is presented, in which a dental site is implicated. a review of the evidence was undertaken. a wide range of dental procedures had been implicated. in some cases the brain isolate was not of dental origin. in many, the diagnosis was one of exclusion. in order to confirm the role of odontogenic infection in the pathogenesis of brain abscess, modern sampling tech ...200111354924
amine and tin fluoride inhibition of streptococcus sanguis adhesion under continuous flow.this study evaluated the ability of topically applied amine fluoride (amf) and amf-tin fluoride to inhibit the adhesion of streptococcus sanguis within a parallel plate flow cell system. one of three amf compounds and two tin fluoride preparations significantly reduced the net bacterial adhesion to conditioned glass over a 1-h period. tin(iv) fluoride inhibited s. sanguis adhesion to the greatest extent, and this was shown to be dependent on the formation of the conditioning film prior to agent ...200111358541
antibacterial activity of particulate bioglass against supra- and subgingival bacteria.particulate bioglass is a bioactive material used in the repair of periodontal defects. this material undergoes a series of surface reactions in an aqueous environment which lead to osseointegration. the aim of this study was to determine whether these reactions exerted an antibacterial effect on a range of oral bacteria. streptococcus sanguis, streptococcus mutans and actinomyces viscosus were suspended in nutrient broth (nb), artificial saliva (as) or dulbecco's modified eagle medium plus 10% ...200111374470
predominant cultivable subgingival microbiota of healthy and hiv-infected ethnic chinese.although clinically distinctive types of periodontal disease are known to be associated with hiv infection, the pathogenesis remains unclear. in the present study, the subgingival microflora of 21 hiv-infected and 11 hiv-free ethnic chinese were studied using the direct microscopic and anaerobic culture methods. motile curved rods were found to be three times higher in the hiv-infected group under direct microscopy. otherwise, there were no significant differences between the diseased and health ...200111398993
the effect of oral bacteria on candida albicans germ-tube formation.a total of eight bacterial isolates belonging to six species, and a select group of 12 oral candida albicans isolates, were used to study the effect of bacteria on germ-tube formation. briefly, each bacterial suspension (10(5-6) cells/ml) was mixed with a c. albicans suspension (10(7) cells/ml) and incubated at 37 degrees c for 90 min with bovine serum, and the percentage germ-tube-positive candida cells was quantified using a haemocytometer, under light microscopy. in general, out of eight bact ...200111398996
investigation of bacteremia after orthodontic banding and debanding following chlorhexidine mouth wash application.this study investigates the prevalence of bacteremia after orthodontic banding and debanding, following the application of a 0.2% chlorhexidine gluconate mouthwash. the banding and debanding groups were each composed of 40 young adult patients. in the banding group, patients were asked to rinse their mouth with chlorhexidine gluconate for 60 seconds just prior to fitting of the bands. in the debanding group, they were asked to use the mouthwash immediately before removal of bands and brackets. i ...200111407771
inhibitory effects of moabs against a surface protein antigen in real-time adherence in vitro and recolonization in vivo of streptococcus mutans.a surface protein antigen (pac) of streptococcus mutans, particularly the a-region of the molecule, has been reported to interact with salivary components on the tooth surface. it might be a candidate antigen inducing the production of antibodies against the adherence of s. mutans to the tooth surface. we investigated the effects of monoclonal antibodies (moabs) obtained by immunization of synthetic pac peptides that completely correspond to the amino acid sequence of part of the a-region. these ...200111439156
phenotypic and genotypic diversity of streptococcus sanguis in infants.streptococcus sanguis comprises a heterogeneous group of oral streptococci indigenous to the oral cavity of humans. a total of 289 isolates from an infant population (n=37) were tentatively identified as s. sanguis on the basis of the distinctive colony morphology as shown on mm10-sucrose non-selective medium. these isolates were divided into four biovars of s. sanguis as determined by an extended panel of biochemical attributes. chromosomal dna was extracted from each isolate, and an ap-pcr fin ...200111442849
endocarditis caused by penicillin-resistant viridans streptococci: 2 cases and controversies in therapy.although penicillin-resistant viridans streptococci have been isolated from samples from the mouth, blood, and wounds in increasing numbers, viridans streptococci isolated from patients with endocarditis have remained sensitive to penicillin for the past 5 decades. we report the cases of 2 patients with penicillin-resistant viridans streptococcal endocarditis, review 6 other cases from the literature, and summarize 2 studies that used an animal model of penicillin-resistant viridans streptococca ...200111462199
do viridans streptococci cause pneumonia in children? 200111465856
enterococcus faecalis--a mechanism for its role in endodontic failure.the aim of this study was to identify a possible mechanism that would explain how e. faecalis could survive and grow within dentinal tubules and reinfect an obturated root canal.200111482724
bacterial detachment from salivary conditioning films by dentifrice supernates.this study compared the detachment by supernates of nine different dentifrices of four oral bacterial strains adhering to a salivary pellicle in a parallel plate flow chamber. ultra-thin bovine enamel slabs were coated for 1.5 h with human whole saliva. following buffer rinsing, a bacterial suspension of streptococcus oralis, streptococcus sanguis, streptococcus mutans or actinomyces naeslundii was perfused through the flow chamber at a shear rate of 30 s-1 for four hours, and the number of adhe ...200211507932
antibacterial activity of hydrastis canadensis extract and its major isolated alkaloids.the antibacterial activity of extract and isolated major alkaloids (berberine, beta-hydrastine, canadine and canadaline) of hydrastis canadensis l. (ranunculaceae) was evaluated against 6 strains of microorganism: staphylococcus aureus (atcc 25 993 and atcc 6538p), streptococcus sanguis (atcc 10 556), escherichia coli (atcc 25 922), pseudomonas aeruginosa (atcc 27 853). bactericidal activity was evaluated by contact test by measuring the "killing time" on a low density bacterial inoculum, and ba ...200111509983
adherence of streptococcus sanguis.the presence of adhesins on the cell surface of s. sanguis enables the organism to grow and survive in the oral cavity. many workers are now actively involved in attempting to characterise these adhesins and the molecular basis for adherence of various streptococci. the complexity of the adhesion processes is underlined by the many varied opinions as to the nature of the molecular components and biochemical interactions that are involved. understanding the molecular mechanisms involved in bacter ...198611547805
the ability of direct fluorescence-based, two-colour assays to detect different physiological states of oral streptococci.to investigate the ability of six fluorescent-based, two-colour viability assays to detect different physiological growth stages of two oral streptococci species.200111555201
alteration of the coadherence of candida albicans with oral bacteria by dietary sugars.interactions between bacterial oral flora and candida albicans are important in denture plaque formation. this study therefore first aimed to quantify the coadherence of c. albicans and bacteria by the use of a bioluminescent adenosine triphosphate (atp) assay based on the firefly luciferase-luciferin system. the second aim was to examine the effect of i) dietary sugars (used for preculture) and ii) enzymatic digestion of fungi on the coadherence. when yeast was preincubated in yeast nitrogen ba ...200111555304
actinomyces naeslundii fimbrial protein orf977 shows similarity to a streptococcal adhesin.the nucleotide sequence of the chromosomal dna, upstream of actinomyces naeslundii t14v fimbrial gene fima, was determined. one open reading frame (orf977) encoding 977 amino acids was found, preceded by a gene homologous to elongation factor tu. database searches revealed that orf977 was homologous to csha, a streptococcus gordonii protein involved in cell adhesion. previous studies had already determined two genes in the type 2 fimbrial gene cluster of a. naeslundii t14v: the structural subuni ...200111555311
phosphorylcholine-dependent cross-reactivity between dental plaque bacteria and oxidized low-density lipoproteins.antibodies reactive with phosphorylcholine (pc) are ubiquitous in human sera, but the antigens stimulating their production and their function are not clear. previous studies have shown that a significant proportion of dental plaque bacteria contain pc as determined by reactivity with pc-specific mouse myeloma proteins and monoclonal antibodies. additionally, serum antibody concentrations of immunoglobulin (igg) g anti-pc are higher in sera of individuals who have experienced periodontal attachm ...200111598029
in vitro antimicrobial activities of bakuchiol against oral microorganisms.bakuchiol was isolated from the seeds of psoralea corylifolia, a tree native to china with various uses in traditional medicine, followed by extraction with ether and column chromatography combined with silica gel and octyldecyl silane. in this study, the antimicrobial activities of bakuchiol against some oral microorganisms were evaluated in vitro. the cell growth of streptococcus mutans was inhibited in a bakuchiol concentration-dependent manner, and growth of s. mutans was completely prevente ...200111600349
susceptibilities of oral bacteria and yeast to mammalian cathelicidins.the effects of cathelicidins against oral bacteria and clinically important oral yeasts are not known. we tested the susceptibilities of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, streptococcus sanguis, candida krusei, candida tropicalis and candida albicans to the following cathelicidins: fall39, smap29, and cap18. smap29 and cap18 were antimicrobial, whereas fall39 did not exhibit antimicrobial activity. future studies are needed to determine the p ...200111600383
cariogenic potential of lactosylfructoside as determined by acidogenicity of oral streptococci in vitro and human dental plaque in situ.the cariogenic potential of lactosylfructoside [o-beta-d-galactopyranosyl-(1-->4)-o-alpha-d-glucopyranosyl-(1<-->2)-beta-d-fructofuranoside] was estimated by experiments on oral streptococci in vitro and human dental plaque in situ. lactosylfructoside was unable to support growth of the strains of streptococcus mutans and s. sobrinus used in this study. however, it was able to support growth of strains of s. sanguis, s. mitis and s. oralis. acid was produced rapidly by cell suspensions of s. ora ...200111641569
plaque removal characteristics of electric toothbrushes using an in vitro plaque model.the purpose of the study was to investigate and quantify the efficacy of plaque removal by commercially available electric toothbrushes using an in vitro system based on artificial plaque grown on glass slides.200111686826
proteolytic degradation of oral biofilms in vitro and in vivo: potential of proteases originating from euphausia superba for plaque control.this paper deals with enzymatic removal of dental plaque, in vitro as well as in vivo, using proteases from the antarctic krill shrimp (euphausia superba), referred to as krillase. krillase exhibits both endo- and exopeptidase activity but has no microbicidal effect. in model systems with pure cultures of oral microorganisms. krillase demonstrated inhibition of microbial adhesion to saliva-coated hydroxyapatite. furthermore, a protocol for the growth of reproducible in vitro plaque films has bee ...200111695752
co-adhesion and removal of adhering bacteria from salivary pellicles by three different modes of brushing.this study compares removal of pairs of co-adhering and non-co-adhering oral actinomyces and streptococci by hand, electric and sonic brushing from salivary pellicles. in addition, re-deposition of a co-adhering and non-co-adhering streptococcal strain to brushed pellicles was studied. first, actinomyces were allowed to adhere to a pellicle in a flow chamber, after which streptococci suspended in saliva were perfused through the chamber at 33 c. pellicles with adhering bacterial pairs were brush ...200111695753
plaque formation on surface modified dental implants. an in vitro study.bacterial adhesion on titanium implant surfaces has a strong influence on healing and long-term outcome of dental implants. parameters like surface roughness and chemical composition of the implant surface were found to have a significant impact on plaque formation. the purpose of this study was to evaluate the influence of two physical hard coatings on bacterial adhesion in comparison with control surfaces of equivalent roughness. two members of the oral microflora, streptococcus mutans and str ...200111737097
dysregulation of monocyte oxidative burst in streptococcal endocarditis.streptococcal subacute endocarditis is characterized by low-grade systemic inflammation. although structural cardiac defects are pivotal, phagocytic cells, i.e. monocytes and neutrophils, are involved in the induction and the course of bacterial endocarditis. decreased production of reactive oxygen metabolites was described in long-lasting infections. we hypothesized that the oxidative burst of phagocytes induced by the infecting organism is defective in patients with streptococcal endocarditis.200111737229
association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid.the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions.200111737506
anti-fibrin antibody binding in valvular vegetations and kidney lesions during experimental endocarditis.in streptococcus sanguinis (sanguis) induced experimental endocarditis, we sought evidence that the development of aortic valvular vegetation depends on the availability of fibrin. endocarditis was induced in new zealand white rabbits by catheter placement into the left ventricle and inoculation of the bacteria. fibrin was localized in the developing vegetation with 99mtechnetium (tc)-labeled anti-fibrin antibody one or three days later. when rabbit anti-fibrin antibody was given intravenously o ...200111762752
bactericidal effect of delmopinol on attached and planktonic streptococcus sanguinis cells.the aim of this investigation was to determine the antibacterial effect of varying concentrations of delmopinol-hcl on attached as well as on planktonic streptooccus sanguinis cells in vitro. in addition, a possible antiadhesive effect on attached micro-organisms was to be investigated. s. sanguinis cells were allowed to attach to glass surfaces. these as well as planktonic cells were exposed to delmopinol-hci in concentrations ranging from 0.2% to 0.00005% for 2 min. the percentage of vital bac ...200111767281
[effects of streptococcus sanguis on blood composition, blood pressure and cardiac dysfunction in rabbis].to investigate effect of streptococcus sanguis on blood cell and cardiopulmonary changes.200111769649
[cloning and analyzing of the conserved protein gene in streptococcus sanguinis atcc10556].to clone and analyze the conserved protein gene in streptococcus sanguinis(s.s) atcc10556.200111774210
[implication and mode of action of infectious agents in the formation of atheromatous plaques. infection and atherosclerosis].different kinds of infectious agents seem to be implied in the atherosclerotic process. indeed, some bacteria and viruses have been identified in atherosclerotic lesions: chlamydia pneumoniae, helicobacter pylori, streptococcus sanguis, porphyromonas gingivalis, herpes simplex viruses type 1 and 2, coxsakievirus b and hepatitis virus a. by their ability to induce antigenic and functional changes in the cells of the vascular wall (endothelial cells, smooth muscle cells, monocytes/macrophages, and ...200111776694
[regulation of adherence to serum-coated hydroxyapatite by streptococcus sanguis].to investigate the effects of ph and calcium iron on adhesion of streptococcus sanguis to serum-coated hydroxyapatite.199911776933
[clubbed fingers]. 200111794900
the predominant microflora of nursing caries lesions.the predominant microflora recovered from infected dentine of 52 carious teeth from 14 children with nursing caries was determined using both selective and non-selective media for the isolation of specific genera and acidified media (ph 5.2) to isolate the predominant aciduric microorganisms, and compared with the microflora of sound enamel surfaces in caries-free children. streptococcus mutans formed a significantly greater proportion of the lesion flora while streptococcus oralis, streptococcu ...200111799279
er:yag and alexandrite laser radiation propagation in root canal and its effect on bacteria.the objective of this study was to compare the bactericidal effect of the er:yag (wavelength 2.94 microm) and the alexandrite (wavelength 0.75 microm) laser radiation. the spreading laser energy in the surrounding hard dental tissues round the root canal was evaluated and the bactericidal effect of both these different laser wavelengths was analyzed.199911800099
antimicrobial activity of four root canal sealers against endodontic pathogens.the antibacterial effects of various types of widely used endodontic sealers have not been compared systematically on facultative or obligate anaerobic endodontic pathogens. the aim of this study was to evaluate the antimicrobial properties of four commonly used endodontic sealers: two epoxy-resin-based sealers (ah26, ah plus), one zinc-oxide eugenol-based sealer (n2), and one calcium hydroxide-based sealer (sealapex). the testing microbes were four facultative anaerobic species (streptococcus m ...200111800436
adhesion in vitro of oral streptococci to porcelain, composite resin cement and human enamel.we investigated the initial adhesion in vitro of oral streptococci to porcelain inlays. four strains of streptococci, streptococcus mitis nctc12261, streptococcus oralis atcc9811, streptococcus sanguis atcc10556 and streptococcus sobrinus omz176, were used in this study. the disc specimens were made of porcelain, composite resin cement and human enamel. these specimens, with or without a saliva-coat, were immersed in a suspension of each streptococci strain at 37 degrees c for 1 hr, and the numb ...200011816366
vitality status of microorganisms in infected human root dentine.this experimental study was initiated to establish a method for characterizing the vitality status of bacteria in infected human root dentine by differentiating between viable and dead microorganisms.200211843972
a protocol for polymerase chain reaction detection of enterococcus faecalis and enterococcus faecium from the root canal.the present study was set up to develop a protocol for detection of enterococcus faecalis and enterococcus faecium from the root canal.200211853234
streptococcus sanguis secretes cd14-binding proteins that stimulate cytokine synthesis: a clue to the pathogenesis of infective (bacterial) endocarditis?streptococcus sanguis is the major causative organism of infective (bacterial) endocarditis but, surprisingly, almost nothing is known about how it induces endocardial inflammation. in earlier studies we have shown that many bacteria secrete potent cytokine-inducing or -inhibiting proteins. we have therefore isolated the material secreted by s. sanguis grown on blood agar or in broth culture and have tested its ability to induce human peripheral blood monocytes to synthesize pro-inflammatory cyt ...200211855941
current status of taxonomic groups of oral streptococci in endocarditis. can virulence factors discriminate between endocarditis and non-endocarditis strains?objective: infective endocarditis is frequently caused by oral streptococci, especially streptococcus sanguis. in this group, many strains have recently been reclassified on the basis of new taxonomic schemes. the purpose of this study was to classify oral streptococci from patients with infective endocarditis and, further, to assess the importance of specific virulence factors for the development of streptococcal endocarditis. methods: twenty-eight previously identified and 10 new streptococcal ...199911856221
in vitro activity of quinupristin/dalfopristin against selected bacterial pathogens isolated in italy.objective: to evaluate the activity of quinupristin/dalfopristin, a new injectable streptogramin, against 732 clinical strains recently isolated in italy. methods: susceptibility tests were performed according to nccls-guided mic methodology. pathogens included in the evaluation included 108 staphylococcus aureus isolates, 124 coagulase-negative staphylococcal isolates, 158 streptococcus pyogenes isolates, 30 streptococcus agalactiae isolates, 30 b-hemolytic streptococcal isolates, 18 streptococ ...199911856292
initial characterization of the streptococcus gordonii htpx gene.examination of the streptococcus gordonii chromosomal region, which lies immediately upstream of the glucosyltransferase positive regulatory determinant rgg, revealed two open reading frames. based on nucleotide sequences, these genes were similar to the listeria monocytogenes lema gene, which is involved in antigen presentation, and the escherichia coli htpx heat shock gene, which has an unknown function. northern hybridization analysis indicated that s. gordonii lema and htpx genes were associ ...200211860552
the effect of tissue molecules on bacterial invasion of dentine.bacterial invasion of dentinal tubules is a critical step in the pathogenesis of dental caries and pulp and periapical disease. the purpose of this study was to determine the effect some molecules commonly found in saliva and dentinal tubule fluid may have on the bacterial invasion of dentine. the results showed that invasion of streptococcus gordonii or enterococcus faecalis cells was inhibited when the bacterial cells were in solution with mucin, immunoglobulin g (igg) and serum, and this was ...200211860553
streptococcus sinensis sp. nov., a novel species isolated from a patient with infective endocarditis.a bacterium was isolated from the blood culture of a patient with infective endocarditis. the cells were facultative anaerobic, nonsporulating, gram-positive cocci arranged in chains. the bacterium grows on sheep blood agar as alpha-hemolytic, gray colonies of 0.5 to 1 mm in diameter after 24 h of incubation at 37 degrees c in ambient air. growth also occurs in 10 or 40% bile and on bile esculin agar but not in 6% nacl. no enhancement of growth is observed in 5% co(2). it is nongroupable with la ...200211880397
subgingival microbiota of indigenous indians of central america.to define the subgingival microbial profiles of adult subjects from a previously identified rural community of indigenous indians in guatemala, central america.200211895544
fluoride and organic weak acids as respiration inhibitors for oral streptococci in acidified environments.oxygen metabolism (respiration) of streptococcus mutans gs-5 involving nadh oxidases, mainly of the h(2)o-producing type, was found to be acid sensitive, as was nadh oxidase activity of cell extracts. respiration of intact cells in acidified media was also highly sensitive to fluoride, with a 50% inhibitory concentration of about 0.02 mm at ph 4. in contrast, nadh oxidases in cell extracts were fluoride insensitive. fluoride inhibition of respiration of intact cells was related to weak-acid effe ...200211929560
[therapeutic impact of streptococcal and enterococcal bacteremia in hematology patients].from january 1999 to may 2000 (17 months), 21 strains of streptococci and four strains of enterococci have been isolated from 74 blood cultures in 25 infectious episodes in hematologic patients. they concerned 21 patients, of 21 to 77 years old. these patients suffered from acute leukaemia (14 cases), chronic lymphoid leukaemia (two cases), non-hodgkin's lymphoma (two cases) or myeloma (three cases). seventeen patients displayed a single streptococcal or enterococcal episode, two had two episode ...200211980330
asymmetric primary ringless repair of mitral valve endocarditis: a case report.the case is presented of a patient with a large mitral valve vegetation. a technique of mitral valve repair involving ringless annuloplasty repair following bileaflet resection of the infected regions of the valve is described. no pledgets or prosthetic material were used in the repair. the patient recovered uneventfully, with trivial mitral regurgitation documented on post-repair echocardiography. this technique may suggest a method for early intervention in mitral endocarditis.200212000175
particulate bioglass reduces the viability of bacterial biofilms formed on its surface in an in vitro model.45s5 bioglass is a bioactive implant material which, in its particulate form, is used in the repair of periodontal defects. the surface reactions undergone by this material in an aqueous environment may exert an antibacterial effect that would be beneficial to periodontal surgical treatment. biofilms of streptococcus sanguis, an early plaque former, and mixed species biofilms from a salivary inoculum grown under conditions similar to those associated with periodontal implants, were grown on part ...200212005145
simultaneous measurement of the viability, aggregation, and live and dead adherence of streptococcus crista, streptococcus mutans and actinobacillus actinomycetemcomitans in human saliva in relation to indices of caries, dental plaque and periodontal disease.salivary proteins have multiple functions and many share similar functions, which may be why it has been difficult to relate variations in their concentrations to oral health and ecology. an alternative is to focus on variations in the major functions of saliva. an hydroxyapatite-coated microplate model has been developed that simultaneously measures saliva-promoted bacterial viability, bacterial aggregation, and live and dead bacterial adherence, while simulating oral temperature and shearing f ...200212015215
the antimicrobial properties of a urea-based handwash lotion with triclosan.the purpose of this study was to determine and compare the antimicrobial action of a urea-based handwash lotion with triclosan with a lipid-depleting detergent-based handwash product with 4% chlorhexidine gluconate. the zones of inhibition produced by the two handwash lotions and the one produced by the positive control were similar against all strains of bacteria tested, indicating that a urea-based handwash lotion with triclosan is as effective as a lipid-depleting handwash lotion in inhibitin ...200112024756
effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms.antimicrobial effects of fluoride in vivo remain contentious. previous studies suggested that 1 mm naf reduced acid production from glucose, and prevented the enrichment of bacteria associated with caries in a chemostat model. the present study examines the effects of a lower fluoride concentration (0.53 mm, 10 ppm naf) in both biofilm and planktonic microbial communities. nine oral species were grown at ph 7.0 and pulsed on 10 successive days with glucose; bacterial metabolism was allowed to re ...200212037363
[clinical and laboratory studies of bacterial adhesion to validate the choice of material for making provisional dentures for patients with periodontal diseases].adhesion of bacteria favoring the development of oral inflammations, including cariesogenic and periodontopathogenic (actinobacillus actinomycetemcommitans, streptococcus sanguis, fusobacterium nucleatum, staphylococcus warneri) and yeast fungi (candida albicans), to 13 materials used for making provisional dentures was studied. adhesion of all the studied bacteria and fungi to russian material esterfil foto was the minimum. clinical use of this material in patients with chronic generalized peri ...200212056141
[the study of precedent subgingival flora colonization on four kinds of crown and bridge restorative materials].the purpose of this in vitro study was to investigate the effects of microbial colonization on different crown or fixed bridge-used restorative materials and discuss the standards for evaluating those materials microscopically. four kinds of restorative materials such as sda-ii type medium alloy, ceramic-fused-to-metal material, heat cure composite resin for crown restoration, plat castable ceramics and four species of precedent subgingival flora (a. viscosus, f. nucleterm, c. ochracea, s. sangu ...199812078179
henoch-schonlein purpura secondary to subacute bacterial endocarditis.henoch-schönlein purpura (hsp), a systemic, small-vessel vasculitic syndrome, is characterized by a nonthrombocytopenic purpuric rash, arthralgia, abdominal pain, and nephritis. these signs and symptoms may occur in any order, and not all are necessary for the diagnosis. although most common in 4- to 7-year-olds, hsp is well documented in adults and is often preceded by a history of mucosal-based infections, especially of the upper respiratory tract. we report a case of hsp that occurred coincid ...200212080945
a role for glycoprotein ib in streptococcus sanguis-induced platelet aggregation.numerous studies have implicated bacteria in cardiovascular disease, but there is a paucity of information on the mechanism involved. in this study we show how the common oral bacterium streptococcus sanguis can directly interact with platelets, resulting in activation and aggregate formation. platelet aggregation was dependent on glycoprotein iib/iiia (gpiib/iiia) and thromboxane. platelets could also directly bind to s sanguis, but this interaction was not inhibited by gpiib/iiia antagonists. ...200212091342
roles of salivary proteins in the adherence of oral streptococci to various orthodontic brackets.knowledge of salivary pellicles on orthodontic brackets provides a better understanding of microbial adherence. the aim of this study was to analyze the effects of bracket pellicles on the adherence of streptococcus gordonii and streptococcus mutans. bracket pellicles were formed by the incubation of 4 kinds of orthodontic brackets with unstimulated whole saliva for 2 hrs, and analyzed by electrophoresis, immunodetection, and amino acid analysis. binding assays were then performed by the incubat ...200212097434
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