Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a simple and rapid immunochromatographic strip test for detecting antibody to porcine reproductive and respiratory syndrome virus. | porcine reproductive and respiratory syndrome is rapidly gaining worldwide importance as one of the most economically significant diseases of swine. the antibody of porcine reproductive and respiratory syndrome virus (prrsv) is detected currently by the combined use of an enzyme-linked immunosorbent assay, serum neutralization test, immunoperoxidase monolayer assay, indirect immunofluorescent antibody test. these methods are time-consuming and require specialized equipment operated by trained te ... | 2008 | 18619681 |
| norepinephrine to increase blood pressure in endotoxaemic pigs is associated with improved hepatic mitochondrial respiration. | low blood pressure, inadequate tissue oxygen delivery and mitochondrial dysfunction have all been implicated in the development of sepsis-induced organ failure. this study evaluated the effect on liver mitochondrial function of using norepinephrine to increase blood pressure in experimental sepsis. | 2008 | 18625036 |
| levamisole synergizes proliferation of intestinal iga+ cells in weaned pigs immunized with vaccine candidate f4ac+ nonenterotoxigenic escherichia coli strain. | levamisole (2, 3, 5, 6-tetrahydro-6-phenylimidazole 2,1-b thiazole) is a well-known nonspecific stimulator of host defence mechanisms. in previous investigations, we have found that levamisole acts on cell-mediated immunity in challenge-induced porcine postweaning colibacillosis (pwc). we assume that levamisole could also act synergistically on humoural immune response when applied as an adjuvant with vaccine candidate strains for oral immunization of weaned pigs against pwc. the influence of le ... | 2008 | 18638293 |
| selection and persistence of ctx-m-producing escherichia coli in the intestinal flora of pigs treated with amoxicillin, ceftiofur, or cefquinome. | extended-spectrum beta-lactamases (esbls), mainly of the ctx-m family, have been associated with escherichia coli strains of animal origin in europe. an in vivo experiment was performed to study the effects of veterinary beta-lactam drugs on the selection and persistence of esbl-producing e. coli in the intestinal flora of pigs. twenty pigs were randomly allocated into three treatment groups and one control group. all pigs were inoculated intragastrically with 10(10) cfu of a nalidixic acid (nal ... | 2008 | 18644956 |
| risk factors for salmonella and hygiene indicators in the 10 largest belgian pig slaughterhouses. | a survey was conducted to collect data on salmonella prevalence, escherichia coli counts (eccs), and aerobic bacteria colony counts (accs) on pig carcasses after chilling at the 10 largest belgian pig slaughterhouses during 2000 through 2004. potential risk factors of contamination associated with production parameters, technical descriptions of the installations, and cleaning and disinfection methods were assessed during investigations in the slaughterhouses. these variables were used first in ... | 2008 | 18680928 |
| in vivo and in vitro antibacterial efficacy of pds plus (polidioxanone with triclosan) suture. | this study evaluated the efficacy of polydioxanone suture with and without triclosan against gram-positive and gram-negative bacteria in vitro and in vivo. | 2008 | 18687027 |
| activated protein c protection from lung inflammation in endotoxin-induced injury. | we studied the protection of recombinant human activated protein c (rhapc) in endotoxin-induced lung inflammation and injury and whether this effect is correlated with modulation of lung matrix metalloproteinase (mmp) activity. we randomly assigned 12 large white pigs to receive intravenous escher-ichia coli lipopolysaccharide (lps; 40 mu g/kg/hr), rhapc (24 mu g/ kg/hr), or both. we monitored respiratory mechanics and function, cell counts, and cytokine concentrations in bron-choalveolar lavage ... | 2008 | 18703750 |
| farm-level impact of therapeutic antimicrobial use on antimicrobial-resistant populations of escherichia coli isolates from pigs. | we investigated the farm-level impact of the use of several different antimicrobial agents on the population of antimicrobial-resistant commensal bacteria of animal origin to appropriately assess the release risk of resistance. this study was carried out based on the results of a survey on the history of antimicrobial drug use in 297 pig farms and antimicrobial susceptibility testing for the 545 escherichia coli isolates (one or two isolates/pig/farm). a comparative analysis with the nonexposed ... | 2008 | 18707531 |
| persistence and spread of gastro-intestinal infections: the case of enterotoxigenic escherichia coli in piglets. | several gastro-intestinal infections in animal husbandry not only greatly reduce the well-being of animals, but also have the potential to cause large economical damage. understanding of the dynamics of such diseases is thus of great importance. in this paper, we focus on within-host dynamics and present a model describing the dynamics of pathogens in the intestine of a single host. our motivation to study the problem stems from the case of enterotoxigenic escherichia coli in newly weaned piglet ... | 2008 | 18709417 |
| penicillin resistance in the intestinal spirochaete brachyspira pilosicoli associated with oxa-136 and oxa-137, two new variants of the class d beta-lactamase oxa-63. | penicillin resistance mediated by beta-lactamase activity has been reported previously in the anaerobic intestinal spirochaete brachyspira pilosicoli, and a novel class d beta-lactamase (oxa-63) hydrolysing oxacillin was described recently in a resistant human strain from france. in the current study, 18 b. pilosicoli strains from australia and papua new guinea were tested for ampicillin and oxacillin susceptibility, and investigated for the presence of the class d beta-lactamase gene blaoxa-63 ... | 2008 | 18719182 |
| the polymeric stability of the escherichia coli f4 (k88) fimbriae enhances its mucosal immunogenicity following oral immunization. | only a few vaccines are commercially available against intestinal infections since the induction of a protective intestinal immune response is difficult to achieve. for instance, oral administration of most proteins results in oral tolerance instead of an antigen-specific immune response. we have shown before that as a result of oral immunization of piglets with f4 fimbriae purified from pathogenic enterotoxigenic escherichia coli (etec), the fimbriae bind to the f4 receptor (f4r) in the intesti ... | 2008 | 18762221 |
| molecular characterization of shiga like toxin-producing escherichia coli (stec) isolates from pigs oedema. | an oedema outbreak occurred in a guwahati pig farm. escherichia coli isolates from different necropsy samples collected from the dead piglets with oedema were characterized to confirm the virulence. | 2008 | 18765880 |
| a survey of fluoroquinolone resistance in escherichia coli and thermophilic campylobacter spp. on poultry and pig farms in great britain. | to estimate the proportions of farms on which broilers, turkeys and pigs were shedding fluoroquinolone (fq)-resistant escherichia coli or campylobacter spp. near to slaughter. | 2008 | 18778293 |
| a tryptophan-enriched diet improves feed intake and growth performance of susceptible weanling pigs orally challenged with escherichia coli k88. | we tested the effect of trp addition to a standard weaning diet and oral challenge with enterotoxigenic escherichia coli k88 (etec) on growth and health of piglets susceptible or nonsusceptible to the intestinal adhesion of etec. sixty-four pigs weaned at 21 d of age were divided into 3 groups based on their ancestry and bw: a control group of 8 pigs fed a basal diet (b), the first challenged group of 28 pigs fed b diet (bch), and the second challenged group of 28 pigs fed a diet with trp (trpch ... | 2009 | 18791156 |
| a rapid serological assay for prediction of salmonella infection status in slaughter pigs using surface plasmon resonance. | we present a rapid surface plasmon resonance-based serological assay for the detection of salmonella typhimurium infection in pigs using the plasmonic((r)) spr device. lipopolysaccharide (lps, 10 microg ml(-1)) from salmonella typhimurium was immobilised by self-assembly on a hydrophobic spr chip. using this lps-coated chip, it was possible to bind and detect the anti-salmonella typhimurium antibodies in serum of pigs infected with the bacteria. the developed spr assay is able to differentiate b ... | 2008 | 18804126 |
| protein isoaspartate methyltransferase prevents apoptosis induced by oxidative stress in endothelial cells: role of bcl-xl deamidation and methylation. | natural proteins undergo in vivo spontaneous post-biosynthetic deamidation of specific asparagine residues with isoaspartyl formation. deamidated-isomerized molecules are both structurally and functionally altered. the enzyme isoaspartyl protein carboxyl-o-methyltransferase (pcmt; ec 2.1.1.77) has peculiar substrate specificity towards these deamidated proteins. it catalyzes methyl esterification of the free alpha-carboxyl group at the isoaspartyl site, thus initiating the repair of these abnorm ... | 2008 | 18806875 |
| evaluation of the risks of shedding salmonellae and other potential pathogens by therapy dogs fed raw diets in ontario and alberta. | dogs that participate in animal-assisted interventions (aais), often called 'therapy dogs', commonly interact with humans whose immune systems are not functioning optimally. the advisability of feeding raw meat (including poultry) to these animals remains a highly contentious issue, in spite of increasing evidence that raw meat is frequently contaminated with salmonella. we set out to determine if consuming raw meat influences the risk of therapy dogs shedding salmonella and other pathogens. two ... | 2008 | 18811908 |
| expression of biologically active recombinant porcine interleukin-12 from escherichia coli. | the control of viral infections is of critical importance to livestock industries worldwide and is highlighted by costly infection outbreaks, such as that seen with foot and mouth disease virus. to ameliorate the impact of increasing problems with viral infections, new vaccine and anti-viral strategies are required and a greater understanding of the anti-viral response is essential. furthermore, in pigs, evidence is still being gathered on the components of a defined anti-viral immune response. ... | 2008 | 18823664 |
| improvement of monocyte secretion function in a porcine pancreatitis model by continuous dose dependent veno-venous hemofiltration. | monocyte and its secreted cytokines play a crucial role in the process of severe acute pancreatitis (sap). it has been known that continuous veno-venous hemofiltration (cvvh) can partially attenuate the inflammatory process by removal of cytokines, but the effect of cvvh on monocyte secretion function remains unknown. this study investigated the effect of different doses of cvvh on monocyte secretion function as well as the plasma cytokine profile in a sap porcine model. | 2008 | 18825644 |
| genotypic and phenotypic characterisation of escherichia coli strains associated with porcine pyelonephritis. | urinary tract infection (uti) is a severe problem in humans as well as in many domestic animals like pigs. the most frequent infectious agent in uti is uropathogenic escherichia coli. such strains have been extensively characterised with respect to virulence and fitness factors as well as clonal type when it comes to human isolates. however, relatively little has been done to characterise the corresponding porcine strains. on this background we have analysed 20 porcine pyelonephritis e. coli str ... | 2009 | 18835113 |
| prevalence of tir gene subtypes in escherichia coli isolates from pigs. | 2008 | 18836158 | |
| [cloning and expression of the pig skeletal muscle musclin gene]. | we found seven tag sequence with high homology in dbest by using human musclin gene, and got its cdna sequence, which consists of 651bp and the open reading frame was 54-452 bp detected by rt-pcr, encoding 132 amino acid residue protein. the new gene has high homology with that of human, mouse and rat, the rate is 87.2%, 77.6% and 77.9%, respectively; the gene fragment was cloned into expression vector pgex-4t-1, and the recombinant was transformed into e. coli bl21. induced by iptg, the fusion ... | 2008 | 18837403 |
| determination of the mimic epitope of the m-like protein adhesin in swine streptococcus equi subsp. zooepidemicus. | the m-like protein, also known as szp, is expressed on the surface of streptococcus equi subsp. zooepidemicus (s. zooepidemicus). previous studies demonstrated that szp is similar to m protein of group a streptococcus in the structure and characteristics of antiphagocytosis. the m protein is an adhesin that can bind to the host cells, however it is not known whether the szp of s. zooepidemicus also functions as an adhesin. we conducted an investigation to determine szp as an adhesin, and one szp ... | 2008 | 18840263 |
| examination of salmonella and escherichia coli translocation from hog manure to forage, soil, and cattle grazed on the hog manure-treated pasture. | use of hog (sus scrofa) manure as a fertilizer is a practical solution for waste re-utilization, however, it may serve as a vehicle for environmental and domestic animal contamination. work was conducted to determine whether pathogens, naturally present in hog manure could be detected in cattle (bos taurus) grazed on the manure-treated pasture, and whether forage contamination occurred. during two 3 mo summer trials manure was applied to yield < or = 124 kg available n per hectare in a single sp ... | 2008 | 18948461 |
| purification, molecular cloning, heterologous expression and characterization of pig cyp1a2. | porcine cytochrome p450 (cyp) 1a2 was purified to electrophoretic homogeneity from the hepatic microsomes of beta-naphthoflavone-treated male pigs. in a reconstituted system, this enzyme showed a good catalytic activity towards caffeine, acetanilide, and methoxyresorufin, all known markers of mammalian cyp1a2. using 3'- and 5'-rapid amplification of coding dna (cdna) ends (race), we amplified from the liver rna of control pigs a full-length 1827 bp cdna containing an open reading frame of 1548 b ... | 2008 | 18949657 |
| evaluation of baits for oral vaccination of european wild boar piglets. | the objective of this study was to develop and evaluate new baits for the oral delivery of vaccine preparations to 2-4 month-old wild boar piglets. baits were prepared using a matrix composed of wild boar feed, wheat flour, paraffin, sacarose and cinnamon-truffle powder attractant with polyethylene capsules dipped into the matrix to introduce vaccine formulation. physical stability studies demonstrated that baits were stable for at least three days at temperatures as high as 42 degrees c. recomb ... | 2009 | 18950820 |
| [construction and immunogenicity of a genetic engineered strain expressing nontoxic st1-lt(b)-alpha-beta fusion protein against diarrhea of piglet]. | we constructed a recombinant strain bl21 (de3) (petst3ltbalphabeta) including st1-lt(b)-alpha-beta fusion gene via molecular technology. the sds-page and western blotting indicated that the st1-lt(b)-alpha-beta fusion protein was highly expressed in escherichia coli and the molecular weight of the fusion protein was about 110 kd. the recombinant strain was induced in different concentrations of lactose and different aeration rate. the optimal culture conditions in 20 l fermentor were 1% inoculat ... | 2008 | 18998533 |
| conserved stx2 phages from escherichia coli o103:h25 isolated from patients suffering from hemolytic uremic syndrome. | one of the main virulence factors produced by shiga toxin-producing escherichia coli is the shiga toxin (stx), which is encoded on lambdoid phages (stx phage). in norway, an outbreak of hemorrhagic colitis and hemolytic uremic syndrome (hus) caused by e. coli o103:h25 was reported during the winter of 2006, but stx(2)-positive isolates were only retrieved from two human samples. | 2008 | 19014273 |
| associations between reported on-farm antimicrobial use practices and observed antimicrobial resistance in generic fecal escherichia coli isolated from alberta finishing swine farms. | multilevel random intercept logistic and poisson regression were used to model associations between various antimicrobial use practices and resistance to individual and multiple antimicrobials among generic fecal escherichia coli isolated from alberta finishing swine. in-feed antimicrobial use in finishers was significantly associated with increased risk of resistance to ampicillin, chloramphenicol, streptomycin, and sulfisoxazole in generic e. coli isolates. chlortetracycline use in grower rati ... | 2009 | 19041147 |
| inhibition of complement and cd14 attenuates the escherichia coli-induced inflammatory response in porcine whole blood. | the innate immune response is a double-edged sword in systemic inflammation and sepsis. uncontrolled or inappropriate activation can damage and be lethal to the host. several studies have investigated inhibition of downstream mediators, including tumor necrosis factor alpha (tnf-alpha) and interleukin-1beta (il-1beta). emerging evidence indicates that upstream inhibition is a better therapeutic approach for attenuating damaging immune activation. therefore, we investigated inhibition of two cent ... | 2009 | 19047409 |
| isolation and characterization of intestinal escherichia coli clones from wild boars in germany. | our understanding of the composition of escherichia coli populations in wild boars is very limited. in order to obtain insight into the e. coli microflora of wild boars, we studied e. coli isolates from the jejunums, ileums, and colons of 21 wild boars hunted in five geographic locations in germany. ten isolates per section were subjected to clonal determination using pulsed-field gel electrophoresis. one representative isolate per clone was further investigated for virulence traits, phylogeneti ... | 2009 | 19060173 |
| supplementation of diets for gestating sows with zinc amino acid complex and gastric intubation of suckling pigs with zinc-methionine on mineral status, intestinal morphology and bacterial translocation in lipopolysaccharide-challenged early-weaned pigs. | eighty male pigs from 20 litters were used to evaluate dietary addition of 250mg/kg of zn from zinc amino acid complex (znaa) to sows during the last trimester of gestation and gastric intubation of 40mg zn from soluble zinc methionine (znmet) to suckling pigs at birth and on day 7 and 14 (weaning) on small intestinal morphology, zn status and bacterial translocation in early-weaned pigs. at weaning, pigs were challenged with an intramuscular injection of saline without or with 120microg/kg bw o ... | 2009 | 19062056 |
| characterization of monoclonal antibody against replication-associated protein of porcine circovirus. | the replication-associated (rep) protein of porcine circovirus (pcv) was suggested to play an essential role in the replication and translation of viral dna. in this study, one monoclonal antibody (mab) specific for rep protein of porcine circovirus type 1 (pcv1), two mabs against rep protein of porcine circovirus type 2 (pcv2), and five mabs to both rep protein of pcv1 and pcv2 were generated using, respectively, rep protein of pcv1 and pcv2 expressed in escherichia coli as an immunogen. wester ... | 2009 | 19072659 |
| biorecognition of escherichia coli k88 adhesin for glycated porcine albumin. | escherichia coli (e. coli) that expresses galactose-reactive lectins, like k88 adhesin, causes high mortality among piglets. carbohydrates that compete for adhesion could serve as an alternative for disease prevention. porcine serum albumin (psa) was modified by non-enzymatic glycation with lactose to produce psa-lac or psa-glc beta (1-4) gal, as confirmed by reduction of available free amino groups, increased molecular mass and by ricinus communis lectin recognition. e. coli k88 binds to psa-la ... | 2009 | 19101589 |
| expression, purification, and characterization of recombinant ns-1, the porcine parvovirus non-structural protein. | the non-structural protein ns-1 of porcine parvovirus (ppv) could be a useful antigen for differentiating pigs infected with ppv from those vaccinated with inactivated whole virus. ns-1 was expressed in escherichia coli using the pet-32a (+) vector. the fusion protein, which was expressed at a high level, was similar antigenically to the native ns-1 protein as determined by western blot assay using polyclonal antibodies from pigs vaccinated with alive ppv vaccine. a simple procedure was used to ... | 2009 | 19101593 |
| characterization of a novel macrolide efflux gene, mef(b), found linked to sul3 in porcine escherichia coli. | the aim of this study was to characterize a putative novel macrolide efflux gene located in the vicinity of sul3 in porcine escherichia coli. | 2009 | 19131424 |
| genetic variation at the alpha-1-fucosyltransferase (fut1) gene in asian wild boar and chinese and western commercial pig breeds. | escherichia coli f18 bacteria producing enterotoxins and/or shigatoxin (etec/stec) are main pathogens that cause oedema disease and postweaning diarrhoea in piglets, and alpha-1-fucosyltransferase (fut1) gene has been identified as a candidate gene for controlling the expression of etec f18 receptor. the genetic variations at nucleotide position 307 in open reading frame of fut1 gene in one wild boar breed and 20 western commercial and chinese native pig breeds were investigated by polymerase ch ... | 2008 | 19134079 |
| farm-level risk factors for fluoroquinolone resistance in e. coli and thermophilic campylobacter spp. on finisher pig farms. | logistic regression, supported by other statistical analyses was used to explore the possible association of risk factors with the fluoroquinolone (fq)-resistance status of 108 pig finisher farms in great britain. the farms were classified as 'affected' or 'not affected' by fq-resistant e. coli or campylobacter spp. on the basis of isolation of organisms from faecal samples on media containing 1 mg/l fq. the use of fq was the most important factor associated with finding resistant e. coli and/or ... | 2009 | 19134238 |
| defatted bovine colostrum-supplemented diet around weaning improves exocrine pancreatic secretion by means of volume, digestive enzymes, and antibacterial activity. | exocrine pancreatic secretion contributes to limit pathogenic bacteria-associated diarrhea. bovine colostrum, used in the treatment of diarrhea, reduces symptoms originating from gut pathogenic bacteria overgrowth. we hypothesized that bovine colostrum may stimulate the exocrine pancreatic secretion. | 2009 | 19136910 |
| associations between antimicrobial resistance phenotypes, antimicrobial resistance genes, and virulence genes of fecal escherichia coli isolates from healthy grow-finish pigs. | escherichia coli often carries linked antimicrobial resistance genes on transmissible genetic elements. through coselection, antimicrobial use may select for unrelated but linked resistance or virulence genes. this study used unconditional statistical associations to investigate the relationships between antimicrobial resistance phenotypes and antimicrobial resistance genes in 151 e. coli isolates from healthy pigs. phenotypic resistance to each drug was significantly associated with phenotypic ... | 2009 | 19139228 |
| chitosan-alginate microcapsules for oral delivery of egg yolk immunoglobulin (igy): in vivo evaluation in a pig model of enteric colibacillosis. | in our previous study, the applicability of chitosan-alginate microcapsules for oral delivery of egg yolk immunoglobulin (igy) was established in a simulated gastrointestinal tract environment. the objective of the present study was to evaluate the protective efficacy of microencapsulated igy against k88+ etec (enterotoxigenic escherichia coli)-induced diarrhea in 40-day-old pigs. groups of pigs orally challenged with 10(11) cfu/ml of k88+ etec were fed with non-encapsulated igy, microencapsulat ... | 2009 | 19150135 |
| genetic loci involved in rubrifacine production in the walnut pathogen brenneria rubrifaciens. | brenneria rubrifaciens produces a unique red pigment known as rubrifacine that has been hypothesized to play a role in pathogenesis on walnut. analysis of dna flanking the tn5 insertion site in 20 rubrifacine minus (pig(-)) mutants identified three regions required for rubrifacine production. the first region was homologous to nonribosomal peptide synthetases (nrps), the second was homologous to autoinducer synthase genes (expi homologs), and the third region was homologous to the slya gene of c ... | 2009 | 19159306 |
| transmission of incn plasmids carrying blactx-m-1 between commensal escherichia coli in pigs and farm workers. | ctx-m-1-producing escherichia coli were isolated from 56 pigs, three farm personnel, two manure samples, and two air samples from two danish pig farms where an association between prophylactic ceftiofur use and the occurrence of cephalosporin resistance was previously demonstrated. human, animal, and environmental strains displayed high genetic diversity but harbored indistinguishable or closely related incn plasmids carrying bla(ctx-m-1), indicating that incn plasmids mediating cephalosporin re ... | 2009 | 19188380 |
| investigation of bioaerosols released from swine farms using conventional and alternative waste treatment and management technologies. | microbial air pollution from concentrated animal feeding operations (cafos) has raised concerns about potential public health and environmental impacts. we investigated the levels of bioaerosols released from two swine farms using conventional lagoon-sprayfield technology and ten farms using alternative waste treatment and management technologies in the united states. in total, 424 microbial air samples taken at the 12 cafos were analyzed for several indicator and pathogenic microorganisms, incl ... | 2008 | 19192808 |
| factors related to the carriage of verocytotoxigenic e. coli, salmonella, thermophilic campylobacter and yersinia enterocolitica in cattle, sheep and pigs at slaughter. | a 12-month abattoir study was undertaken from january 2003. we collected 7492 intestinal samples from cattle, sheep and pigs at slaughter. rectal samples were taken from cattle and sheep and caecal samples from pigs. they were examined for verocytotoxigenic e. coli (vtec) o157, salmonella, thermophilic campylobacter and yersinia enterocolitica. data were collected on the animal from which the sample came and this information was analysed to look at potential risk factors for carriage of these or ... | 2009 | 19203406 |
| a pig-human comparative rh map comprising 20 genes on pig chromosome 13q41 that harbours the etec f4ac receptor locus. | the enterotoxigenic escherichia coli (etec) f4ac is a major cause of diarrhoea in newborn and young pigs. the locus for the intestinal etec f4ac receptor (f4acr) has been mapped to pig chromosome (ssc) 13q41 with known homology to human chromosome (hsa) 3q21 and q29. however, the causative gene and mutation(s) remain unknown. the aim of this study was to characterize gene-derived markers on ssc13q41 for fine mapping of the f4acr locus, and construct a high-resolution pig-human comparative map to ... | 2009 | 19207927 |
| concentration and bioavailability of ciprofloxacin and teicoplanin in the cornea. | to investigate the concentration and bioavailability of ciprofloxacin and teicoplanin in the cornea. | 2009 | 19218614 |
| comparison of swabbing and destructive methods for microbiological pig carcass sampling. | to compare the belgian swabbing sampling method for pig carcasses with the reference destructive method with regard to escherichia coli and aerobic plate counts, salmonella and campylobacter prevalence and their relationship. | 2008 | 19241527 |
| phenotypic and genotypic traits of shiga toxin-negative e. coli o157:h7/h(-) bovine and porcine strains. | enterohemorrhagic e. coli (ehec) o157:h7/h(-) (nonmotile) exist as shiga toxin gene (stx)-positive and stx-negative variants in patients and the environment. we analyzed molecular characteristics, phenotypes, and the phylogenetic background of three stx-negative e. coli o157:h7/h(-) strains isolated from cattle and a pig and compared them with those of human ehec and stx-negative e. coli o157:h7/h(-). all three animal strains contained flich7 and two contained eae. one eae-positive strain (o157: ... | 2009 | 19245340 |
| characterization of sulphonamide-resistant escherichia coli using comparison of sul2 gene sequences and multilocus sequence typing. | the sul2 gene encodes sulphonamide resistance (sul(r)) and is commonly found in escherichia coli from different hosts. we typed e. coli isolates by multilocus sequence typing (mlst) and compared the results to sequence variation of sul2, in order to investigate the relation to host origin of pathogenic and commensal e. coli strains and to investigate whether transfer of sul2 into different genomic lineages has happened multiple times. sixty-eight e. coli isolated in denmark and norway from diffe ... | 2009 | 19246754 |
| selection of non-aggregating vh binders from synthetic vh phage-display libraries. | the particular interest in vh antibody fragments stems from the fact that they can rival their "naturally occurring" single-domain antibody (sdab) counterparts (camelid vhhs and shark vnars) with regard to such desirable characteristics as stability, solubility, expression, and ability to penetrate cryptic epitopes and outperform them in terms of less immunogenicity, a much valued property in human immunotherapy applications. however, human vhs are typically prone to aggregation. various approac ... | 2009 | 19252860 |
| antimicrobial resistance in escherichia coli strains isolated from swiss weaned pigs and sows. | based on directive (ec) no 99/2003, monitoring programs on the development of antimicrobial resistance in bacteria from livestock are implemented in many european countries. the aim of the present study was (i) to establish comprehensive baseline data on the antimicrobial resistance situation in escherichia coli isolates obtained from healthy pigs (pooled fecal samples) originating from 60 swiss pig-breeding farms, and (ii) to analyze differences in the resistance frequency between escherichia c ... | 2009 | 19263381 |
| relatedness of escherichia coli strains with different susceptibility phenotypes isolated from swine feces during ampicillin treatment. | the aim of this study was to examine the dynamics of the development of resistance in fecal escherichia coli populations during treatment with ampicillin for 7 days in pigs. before treatment, only 6% of the isolates were ampicillin resistant, whereas more than 90% of the isolates were resistant after days 4 and 7 of treatment. ampicillin-resistant e. coli isolates were mainly multiresistant, and 53% of the isolates from the treated pigs had one phenotype that included resistance to six antibioti ... | 2009 | 19270132 |
| addition of an alginate to a modified zeolite improves hemostatic performance in a swine model of lethal groin injury. | quikclot is a zeolite-based hemostatic agent that can control severe hemorrhage through adsorption of water in an exothermic reaction. ion exchanging the calcium ions in zeolite type 5a with cations of a reduced hydration enthalpy can reduce heat generation, but its effect on the hemostatic efficacy is not clear. we developed a new compound zeolite hemostat and tested it against controls in a modified swine model of battlefield injury. | 2009 | 19276728 |
| a novel endo-glucanase from the thermophilic bacterium geobacillus sp. 70pc53 with high activity and stability over a broad range of temperatures. | a thermophilic geobacillus bacterium secreting high activity of endo-glucanase (ec 3.2.1.4) was isolated from rice straw compost supplemented with pig manure. a full-length gene of 1,104 bp, cela, encoding this glycosyl hydrolase family 5 endo-glucanase of 368 amino acids was isolated. no related gene from geobacillus has been reported previously. the recombinant cela expressed in escherichia coli had an optimal activity at 65 degrees c and ph 5.0, and it exhibited tenfold greater specific activ ... | 2009 | 19296197 |
| prevalence of enterotoxigenic escherichia coli virulence genes from scouring piglets in zimbabwe. | world-wide, enterotoxigenic escherichia coli (etec) and verotoxigenic e. coli (vtec)-induced diarrhea are economically important for porcine producers. our aim was to investigate the prevalence of toxin and fimbrial genes among e. coli isolated from diarrheic piglets from randomly selected piggeries in zimbabwe. we used multiplex pcr for screening sta, stb, lt, and stx-2e toxins. subsequently f4, f5, f6, f18 and f41 fimbriae genes were screened in toxin positive isolates. toxin positive strains ... | 2009 | 19347597 |
| influence of lactobacillus fermentum i5007 on the intestinal and systemic immune responses of healthy and e. coli challenged piglets. | the effect of feeding lactobacillus fermentum i5007 on the immune system of weaned pigs with or without e. coli challenge was determined. twenty-four weaned barrows (6.07 +/- 0.63 kg bw) were randomly assigned to one of four treatments (n = 6) in a factorial design experiment. the first two treatments consisted of healthy piglets with half of the pigs receiving no treatment while the other half was orally administered with l. fermentum i5007 (10(8) cfu/ml) at a daily dose of 20 ml. pigs in the s ... | 2009 | 19347600 |
| lactobacillus isolates from weaned piglets' mucosa with inhibitory activity against common porcine pathogens. | twelve lactobacilli isolates from mucosa of 3-5-week-old weaned pigs were found to exert good antimicrobial activity against common porcine pathogens (s. aureus, b. cereus, e. coli, c. perfringens). two of them produced in addition to lactic acid also considerable amounts of acetic acid, and 6 of them produced hydrogen peroxide and metabolites other than organic acids. isolates 4/26 and 2/25 (identified as l. crispatus or l. amylovorus) were inhibitory against most strains of s. aureus, b. cereu ... | 2009 | 19381487 |
| determination of phosphorylation sites for nadp-specific isocitrate dehydrogenase from mycobacterium tuberculosis. | isocitrate dehydrogenase (icd) catalyzes the oxidative decarboxylation reaction of 2r,3s-isocitrate to yield 2-oxoglutarate in the tricarboxylic acid (tca) cycle. two isoforms of nadp-specific icds with the e.c number 1.1.1.42 have been annotated in the organism mycobacterium tuberculosis, monomeric icd2 and dimeric icd1. blast search against the protein data bank (pdb) database shows a marked similarity between dimeric mycobacterium tuberculosis icd1 sequence and that of sus scrofa, a cytosolic ... | 2009 | 19385702 |
| the food contaminant fumonisin b(1) reduces the maturation of porcine cd11r1(+) intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal etec infection. | consumption of food or feed contaminated with fumonisin b(1) (fb(1)), a mycotoxin produced by fusarium verticillioides, can lead to disease in humans and animals. the present study was conducted to examine the effect of fb1 intake on the intestinal immune system. piglets were used as a target and as a model species for humans since their gastro-intestinal tract is very similar. the animals were orally exposed to a low dose of fb(1) (1 mg/kg body weight fb(1)) for 10 days which did not result in ... | 2009 | 19389343 |
| refined localization of the escherichia coli f4ab/f4ac receptor locus on pig chromosome 13. | diarrhoea in newborn and weaned pigs caused by enterotoxigenic escherichia coli (etec) expressing f4 fimbriae leads to considerable losses in pig production. in this study, we refined the mapping of the receptor locus for etec f4ab/f4ac adhesion (f4bcr) by joint analysis of nordic and swiss data. a total of 236 pigs from a nordic experimental herd, 331 pigs from a swiss experimental herd and 143 pigs from the swiss performing station were used for linkage analysis. genotyping data of six known m ... | 2009 | 19392815 |
| quantification of heparan sulfate disaccharides using ion-pairing reversed-phase microflow high-performance liquid chromatography with electrospray ionization trap mass spectrometry. | the glycosaminoglycan (gag) family of biomacromolecules is composed acidic and linear chains of repeating disaccharide units. quantitative disaccharide composition analysis is essential for the study and characterization of gags. heparan sulfate and heparin consist of multiple disaccharide units and can be well-separated by ion-pairing reversed-phase microflow high-performance liquid chromatography (iprp-mf-hplc). each disaccharide can be detected and its mass confirmed by electrospray ionizatio ... | 2009 | 19402671 |
| comparison of genotypes of escherichia coli strains carrying f18ab and f18ac fimbriae from pigs. | escherichia coli carrying the f18 fimbriae colonize the small intestine of pigs and cause postweaning diarrhea and edema disease. there are 2 closely related antigenic variants of f18, f18ab, and f18ac. while f18ab-positive strains are known to be associated with edema disease, e. coli-carrying f18ac are known to cause diarrhea. one hundred ninety-eight e. coli isolates obtained from cases of diarrhea and edema disease in pigs isolated from feces or intestine were screened for the presence of th ... | 2009 | 19407090 |
| escherichia coli expressing single-chain fv on the cell surface as a potential prophylactic of porcine epidemic diarrhea virus. | porcine epidemic diarrhea virus (pedv) is a causative agent of severe diarrhea which leads to death in piglets. because of the high mortality which is up to 100% in suckling piglets, ped is an important porcine disease in korea. in this study, we developed a prophylactic candidate using single-chain fvs to prevent the pedv infection. scfvs of mouse monoclonal antibody which was verified to neutralize pedv was expressed in escherichia coli expression system. after the confirmation of pedv neutral ... | 2009 | 19428826 |
| escherichia coli nissle 1917 for probiotic use in piglets: evidence for intestinal colonization. | this study was prompted to investigate the intestinal localization and colonization of orally administered escherichia coli nissle 1917 (ecn) in piglets. | 2009 | 19457029 |
| incidence of lameness and abrasions in piglets in identical farrowing pens with four different types of floor. | lameness in piglets is a major animal welfare issue. floor abrasiveness is a common cause of superficial injury in piglets in farrowing pens. the abrasion achieved may act as a gate for infections, which in turn may induce development of infectious arthritis. in this study, the influence of improvements of the floor quality and of increased ratios of straw in identical farrowing pens was measured. | 2009 | 19463187 |
| african swine fever virus ap endonuclease is a redox-sensitive enzyme that repairs alkylating and oxidative damage to dna. | african swine fever virus (asfv) encodes an ap endonuclease (pe296r) which is essential for virus growth in swine macrophages. we show here that the dna repair functions of pe296r (ap endonucleolytic, 3'-->5' exonuclease, 3'-diesterase and nucleotide incision repair (nir) activities) and dna binding are inhibited by reducing agents. protein pe296r contains one intramolecular disulfide bond, whose disruption by reducing agents might perturb the interaction of the viral ap endonuclease with the dn ... | 2009 | 19464038 |
| activation of peroxisome proliferator-activated receptor-gamma potentiates pro-inflammatory cytokine production, and adrenal and somatotropic changes of weaned pigs after escherichia coli lipopolysaccharide challenge. | our previous study demonstrated mrna and protein expression of peroxisome proliferator-activated receptor-g (ppar-g) in the immune system of weaned pigs. in this report, to test the hypothesis that activation of ppar-g in immune system modulates inflammatory response, and adrenal and somatotropic responses associated with immune challenge, we administered intraperitoneally ppar-g agonist and/or antagonist in weaned pigs subjected to escherichia coli lipopolysaccharide (lps) challenge. unexpected ... | 2009 | 19474210 |
| antimicrobial use in the australian pig industry: results of a national survey. | to describe how various antimicrobials are used in commercial pig herds in australia and for what disease conditions. | 2009 | 19489779 |
| ctx-m-type beta-lactamase in escherichia coli isolated from sick animals in korea. | about 408 escherichia coli were isolated from sick farm animals and pets during 2003-2006. of these isolates, four strains showed resistance to third-generation cephalosporins. the bla(ctx-m-14) gene was encountered in three e. coli strains, each of which were isolated from two cows and a dog, respectively, and bla(ctx-m-15) was identified in an e. coli isolated from a pig. all bla(ctx-m) genes were found to be transferred. the pulsed-field gel electrophoresis pattern indicated that the ctx-m-ty ... | 2009 | 19496675 |
| resistance patterns and detection of aac(3)-iv gene in apramycin-resistant escherichia coli isolated from farm animals and farm workers in northeastern of china. | the aminoglycoside apramycin has been used widely in animal production in china since 1999. this study was aimed to investigate the resistance pattern of apramycin-resistant escherichia coli isolated from farm animals and farm workers in northeastern of china during 2004-2007 and to determine whether resistance to apramycin was mediated by plasmid containing the aac(3)-iv gene and the mode for the transfer of genetic information between bacteria of farm animals and farm workers. thirty six e. co ... | 2009 | 19501867 |
| molecular analysis of escherichia coli from retail meats (2002-2004) from the united states national antimicrobial resistance monitoring system. | the origins and virulence potential of meat product-associated escherichia coli are undefined. | 2009 | 19508167 |
| global spread of mobile antimicrobial drug resistance determinants in human and animal escherichia coli and salmonella strains causing community-acquired infections. | antimicrobial drugs used in human infection treatment and animal husbandry may select for drug-resistant bacterial pathogens, which are increasingly observed worldwide. we sought to examine the extent to which identical mobile drug resistance elements are shared across common pathogens isolated from human and animal sources. | 2009 | 19538087 |
| effects of phenyllactic acid on growth performance, nutrient digestibility, microbial shedding, and blood profile in pigs. | ninety weanling pigs in exp. 1 (6.27 +/- 0.73 kg; 21 d of age) and 96 growing pigs in exp. 2 (21.73 kg +/- 1.29 kg; 56 d of age) were used in two 42-d experiments to evaluate the effect of phenyllactic acid (pla) on growth performance, apparent total tract digestibility (attd) of dm and n, fecal ph value, microbial shedding, and blood profiles. in exp. 1, the 3 dietary treatments were 1) negative control (nc), 2) positive control (pc), nc + antibiotics, and 3) pla, nc + 0.5% pla. in exp. 2, diet ... | 2009 | 19542505 |
| expression of porcine parvovirus vp2 gene requires codon optimized e. coli cells. | porcine parvovirus (ppv) is a widespread infectious virus that causes serious reproductive diseases of swine and death of piglets. the gene coding for the capsid protein vp2 of ppv was amplified and inserted into the plasmid pet-32a (+), which was then used to transform escherichia coli rosetta, the capsid protein of ppv was fused to a polyhistidine tag, and the position of the affinity tag is in n-terminus. vp2 was expressed using different expression host bacteria, including e. coli bl21, and ... | 2009 | 19543964 |
| resistance mechanisms and farm-level distribution of fecal escherichia coli isolates resistant to extended-spectrum cephalosporins in pigs in spain. | fecal escherichia coli isolates showing a phenotype of reduced susceptibility or resistance to extended-spectrum cephalosporins are common among pigs in spain. the aim of this study was to describe the main beta-lactam resistance mechanisms carried by these strains and their distribution at farm-level. | 2010 | 19577265 |
| transgenic lettuce producing a candidate protein for vaccine against edema disease. | pig edema disease is a bacterial disease caused by shiga toxin 2e-producing escherichia coli belonging mainly to serotypes o138, o139, and o141. the b subunit of shiga toxin 2e (stx2eb) is a candidate protein for use in a vaccine against edema disease. we produced this protein in transgenic lettuce (lactuca sativa), an edible plant that can be cultivated in a factory setting. in a transient expression system, we found that ntadh 5'-untranslated region (5'-utr) functions as a translational enhanc ... | 2009 | 19584542 |
| evidence of class 1 integron transfer between escherichia coli and salmonella spp. on livestock farms. | a study was conducted to determine if homologous integrons occurred in escherichia coli and salmonella spp. within livestock production sites in the united states and thailand suggesting transfer of genetic resistance elements between those organisms. fecal samples were collected via rectal swabs from live swine in the united states and thailand, and cloacal swabs from live chickens in thailand, and killed chickens at a u.s. abattoir. isolates were derived only from farms harboring both salmonel ... | 2009 | 19630513 |
| distribution of sulfonamide resistance genes in escherichia coli and salmonella isolates from swine and chickens at abattoirs in ontario and québec, canada. | sulfonamide-resistant escherichia coli and salmonella isolates from pigs and chickens in ontario and québec were screened for sul1, sul2, and sul3 by pcr. each sul gene was distributed differently across populations, with a significant difference between distribution in commensal e. coli and salmonella isolates and sul3 restricted mainly to porcine e. coli isolates. | 2009 | 19633109 |
| contributions of o island 48 to adherence of enterohemorrhagic escherichia coli o157:h7 to epithelial cells in vitro and in ligated pig ileal loops. | o island 48 (oi-48) of escherichia coli consists of three functional gene clusters that encode urease, tellurite resistance (te(r)), and putative adhesins iha and aida-1. the functions of these clusters in enterohemorrhagic e. coli (ehec) o157:h7 infection are unknown. deletion mutants for these three regions were constructed and evaluated for their ability to adhere to epithelial cells in vitro and in ligated pig ileal loops. deletion of the te(r) gene cluster reduced the ability of the organis ... | 2009 | 19633120 |
| [the apoptosis of vero cells induced by shiga toxin 2e]. | shiga-like toxin-producing escherichia coli (stec) causes edema disease in piglets and hemolytic uremic syndrome in human. shiga-like toxins (stxs) produced by stec induce mammalian cells death via either necrosis or apoptosis. however, the ability of stx2e, separated from edema disease (stx2e), to trigger apoptosis and the sequence of intracellular signaling events have not yet been completely defined. in this study we investigated the apoptotic effects of stx2e on vero cells. | 2009 | 19637576 |
| effect of dietary level of protein and fiber on the productive performance and health status of piglets. | to study the interaction between the levels of protein and fiber on the productive performance and health status of piglets, ninety-six 35-d-old piglets (9.11 +/- 0.60 kg of bw) were placed in 32 pens of 3 animals each and allotted to 4 dietary treatments for 21 d. the 4 diets were based on rice, dairy products, and soybean meal in a 2 x 2 factorial arrangement of treatments, with 2 levels of cp (15.4 vs. 19.4%, as-fed basis) and 2 levels of dietary fiber [df; low fiber (lf) 5.3% ndf and high fi ... | 2009 | 19648494 |
| effect of immunostimulation by detoxified e. coli lipopolysaccharide combined with inactivated propionibacterium granulosum cells on porcine immunity. | the aim of this experiment was to evaluate the immunomodulating activities of inactivated propionibacterium granulosum cell walls and e. coli lipopolysaccharide (pg/lps) on porcine immunity. piglets were intramuscularly administered pg/lps (1 ml/10 kg body weight) once or twice. the function of natural killer cells, lymphocytes and neutrophils and the adjuvant effect on antibody induction by attenuated classical swine fever virus (csfv) and inactivated mycoplasma hyopneumoniae vaccination were e ... | 2009 | 19652476 |
| effects of adding liquid dl-methionine hydroxy analogue-free acid to drinking water on growth performance and small intestinal morphology of nursery pigs. | this study was conducted to evaluate the effect of adding liquid dl-methionine hydroxy analogue free acid (lma) to drinking water on growth performance, small intestinal morphology and volatile fatty acids in the caecum of nursery pigs. twenty-four crossbred pigs (large white x landrace, bw approximately 18 kg) were divided into three groups with four replications of two piglets each. the piglets received drinking water without (control), with 0.05 or 0.10% lma. the results indicated that adding ... | 2010 | 19663980 |
| epr spectroscopy and electrospray ionization mass spectrometry reveal distinctive features of the iron site in leukocyte 12-lipoxygenase. | the procedure for the expression and purification of recombinant porcine leukocyte 12-lipoxygenase using escherichia coli [k.m. richards, l.j. marnett, biochemistry 36 (1997) 6692-6699] was updated to make it possible to produce enough protein for physical measurements. electrospray ionization tandem mass spectrometry confirmed the amino acid sequence. the redox properties of the cofactor iron site were examined by epr spectroscopy at 25k following treatment with a variety of fatty acid hydroper ... | 2009 | 19683507 |
| assessment of shiga toxin-producing escherichia coli isolates from wildlife meat as potential pathogens for humans. | a total of 140 shiga toxin-producing escherichia coli (stec) strains from wildlife meat (deer, wild boar, and hare) isolated in germany between 1998 and 2006 were characterized with respect to their serotypes and virulence markers associated with human pathogenicity. the strains grouped into 38 serotypes, but eight o groups (21, 146, 128, 113, 22, 88, 6, and 91) and four h types (21, 28, 2, and 8) accounted for 71.4% and 75.7% of all stec strains from game, respectively. eighteen of the serotype ... | 2009 | 19700552 |
| anti-inflammatory effects of c1-inhibitor in porcine and human whole blood are independent of its protease inhibition activity. | c1-inhibitor (c1-inh) is an important biological inhibitor, regulating several protein cascade systems. recent research has shown that the molecule exhibits properties not dependent on its protease inhibition activity. serum and whole blood from pigs and humans were pre-incubated with c1-inh, ic1-inh or the complement inhibitors spice or compstatin. whole, live escherichia coli were then added for further incubation. complement activation, a range of cytokines, chemokines and growth factors, as ... | 2010 | 19710096 |
| a comparison of aflp and eric-pcr analyses for discriminating escherichia coli from cattle, pig and human sources. | amplified fragment length polymorphism (aflp) and enterobacterial repetitive intergenic consensus polymerase chain reaction (eric-pcr) genomic fingerprinting assays were compared for their ability to differentiate escherichia coli isolates obtained from various host sources, and with respect to their pathogenicity. one hundred and ten verotoxigenic, enterotoxigenic and non-pathogenic e. coli isolates obtained from cattle, humans and pigs were used in this study. the aflp assay was shown to be hi ... | 2004 | 19712352 |
| transcriptomics of enterotoxigenic escherichia coli infection. individual variation in intestinal gene expression correlates with intestinal function. | acute secretory diarrhea is a major cause of morbidity and mortality in young animals and humans. deaths result from excessive fluid and electrolyte losses. the disease is caused by non-invasive bacteria such as vibrio cholerae and escherichia coli which produce enterotoxins, however, much less is known about the role of individual host responses. here we report the response of intact porcine small intestinal mucosa to infection with enterotoxigenic e. coli (etec). jejunal segments in four pigle ... | 2010 | 19716242 |
| strain-dependent variability in growth and survival of escherichia coli in agricultural soil. | abstract this study investigated strain-dependent variability in escherichia coli survival in soil, and strain-dependent responses to variations in some soil conditions. collections of e. coli were isolated from swine manure slurry, and from manured soil following 6 days of incubation in the laboratory. the bacteria were fingerprinted by enterobacterial repetitive intergenic consensus-polymerase chain reaction (eric-pcr). during the course of the incubation the composition of the e. coli communi ... | 2003 | 19719611 |
| arginine-vasopressin attenuates beneficial norepinephrine effect on jejunal mucosal tissue oxygenation during endotoxinaemia. | the objective of the present study was to investigate the effects of increasing doses of norepinephrine (ne) with or without arginine-vasopressin (avp) on intestinal oxygen supply and jejunal mucosal tissue oxygen tension in an acute endotoxic pig model. | 2009 | 19720610 |
| occurrence and characteristics of class 1 and class 2 integrons in resistant escherichia coli isolates from animals and farm workers in northeastern china. | the purpose of this study was to investigate the incidence of class 1 and class 2 integrons and the presence of gene cassettes in resistant escherichia coli isolates from farm animals and farm workers in northeastern china during 2004-2007. most of the e. coli isolates from the farm workers and animals were mutiresistant and showed high resistance to aminoglycosides, chloramphenicol, amoxicillin, sulfanilamide, and quinolones. integrons were found in 56.8% of the resistant e. coli isolates from ... | 2009 | 19728782 |
| distant effects of nitric oxide inhalation in endotoxemic pigs. | inhalation of nitric oxide (ino) has distant effects. by a blood- borne factor, ino down-regulates endogenous nitric oxide production in healthy pig lungs, resulting in vasoconstriction in lung regions not directly reached by ino. the aim of this study was to investigate whether ino has distant effects in endotoxemic pig lungs. the hypothesis was that ino down-regulates endogenous no production in lung regions not reached by ino. | 2010 | 19730256 |
| cloning, in vitro expression, and bioactivity of interleukin-18 isolated from a domestic porcine breed found in henan. | to evaluate the effects of recombinant porcine interleukin-18 (rpil-18) on the replication of viruses in host cells and proliferation of lymphocytes, porcine il-18 (pil-18) isolated from a domestic big-white porcine breed found in the henan province (hn) was cloned using a reverse transcriptase-pcr. the cloned hn pil-18 contained an orf of 579 base pairs encoding a 192-amino-acid precursor protein. the amino acid sequence of hn pil-18 was compared with all the other pil-18 amino acid sequences a ... | 2009 | 19732142 |
| characterization of antimicrobial resistance and integrons among escherichia coli isolated from animal farms in eastern china. | a total of 182 escherichia coli isolates from animals, environment and workers of dairy cattle, swine and chicken farms in shandong which locates in eastern china, were investigated for antimicrobial resistance as well as prevalence and the transfer mechanisms of integrons. the results revealed isolates from swine and chicken farm exhibited high levels of resistance to antimicrobial agents. the positive rate of gene cassette of class 1 integron in dairy cattle, swine and chicken farms was 5%, 20 ... | 2010 | 19744463 |
| parallel isotope-based quantification of modified trna nucleosides. | 2009 | 19746495 | |
| evaluation of lactobacillus rhamnosus gg using an escherichia coli k88 model of piglet diarrhoea: effects on diarrhoea incidence, faecal microflora and immune responses. | probiotic bacterium lactobacillus rhamnosus gg (lgg) has been demonstrated to adhere to pig intestinal mucus, and is able to displace and inhibit pathogens, including escherichia coli (e. coli), in vitro. however, currently there are few data concerning the effects of lgg on piglet health. the objectives of this study were to assess the effectiveness of lgg in reducing the incidence and severity of post-weaning diarrhoea in piglets, and to investigate its mechanisms of action. eighteen weaned ba ... | 2010 | 19782483 |
| refined candidate region specified by haplotype sharing for escherichia coli f4ab/f4ac susceptibility alleles in pigs. | infection of the small intestine by enterotoxigenic escherichia coli f4ab/ac is a major welfare problem and financial burden for the pig industry. natural resistance to this infection is inherited as a mendelian recessive trait, and a polymorphism in the muc4 gene segregating for susceptibility/resistance is presently used in a selection programme by the danish pig breeding industry. to elucidate the genetic background involved in e. coli f4ab/ac susceptibility in pigs, a detailed haplotype map ... | 2010 | 19799599 |
| wild boars as reservoirs of extended-spectrum beta-lactamase (esbl) producing escherichia coli of different phylogenetic groups. | esbl-producing e. coli isolates have been isolated from eight of seventy seven faecal samples (10.4%) of wild boars in portugal. the esbl types identified by pcr and sequencing were bla(ctx-m-1) (6 isolates) and bla(ctx-m-1) + bla(tem1-b) (2 isolates). further resistance genes detected included tet (a) or tet (b) (in three tetracycline-resistant isolates), aad a (in three streptomycin-resistant isolates), cml a (in one chloramphenicol-resistant isolate), sul 1 and/or sul 2 and/or sul 3 (in all s ... | 2009 | 19810044 |
| cd14 inhibition efficiently attenuates early inflammatory and hemostatic responses in escherichia coli sepsis in pigs. | sepsis is a severe infection-induced systemic inflammatory syndrome. inhibition of downstream inflammatory mediators of sepsis, e.g., tnf-alpha, has failed in clinical trials. the aim of this study was to investigate the effects of inhibiting cd14, a key upstream innate immunity molecule, on the early inflammatory and hemostatic responses in a pig model of gram-negative sepsis. the study comprised two arms, whole live escherichia coli bacteria and e. coli lipopolysaccharide (lps) (n=25 and n=9 a ... | 2010 | 19841036 |