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complement-related proteins control the flavivirus infection of aedes aegypti by inducing antimicrobial peptides.the complement system functions during the early phase of infection and directly mediates pathogen elimination. the recent identification of complement-like factors in arthropods indicates that this system shares common ancestry in vertebrates and invertebrates as an immune defense mechanism. thioester (te)-containing proteins (teps), which show high similarity to mammalian complement c3, are thought to play a key role in innate immunity in arthropods. herein, we report that a viral recognition ...201424722701
evaluation of the effectiveness of mass trapping with bg-sentinel traps for dengue vector control: a cluster randomized controlled trial in manaus, brazil.the objective of this study was to assess the effectiveness of bg-sentinel (bgs) traps for mass trapping at the household level to control the dengue vector, aedes aegypti (l.), in manaus (brazil) by performing a cluster randomized controlled trial. after an initial questionnaire and baseline monitoring, 6 out of 12 clusters were randomly allocated to the intervention arm, where participating premises received one bgs trap for mass trapping. the other six clusters did not receive traps and were ...201424724291
sublethal effect of pyriproxyfen released from a fumigant formulation on fecundity, fertility, and ovicidal action in aedes aegypti (diptera: culicidae).dengue and dengue hemorrhagic fever are mosquito-borne viral diseases that coincide with the distribution of aedes aegypti (l.), the primary vector in the tropical and semitropical world. with no available vaccine, controlling the dengue vector is essential to prevent epidemics. the effects of the insect growth regulator pyriproxyfen on ae. aegypti adults that survived a treatment with a sublethal dose were investigated in the laboratory, including effects on their reproductive potential. pyripr ...201424724294
genome-wide snps lead to strong signals of geographic structure and relatedness patterns in the major arbovirus vector, aedes aegypti.genetic markers are widely used to understand the biology and population dynamics of disease vectors, but often markers are limited in the resolution they provide. in particular, the delineation of population structure, fine scale movement and patterns of relatedness are often obscured unless numerous markers are available. to address this issue in the major arbovirus vector, the yellow fever mosquito (aedes aegypti), we used double digest restriction-site associated dna (ddrad) sequencing for t ...201424726019
larval competition extends developmental time and decreases adult size of wmelpop wolbachia-infected aedes aegypti.the intracellular endosymbiont wolbachia has been artificially transinfected into the dengue vector aedes aegypti, where it is being investigated as a potential dengue biological control agent. invasion of wolbachia in natural populations depends upon the fitness of wolbachia-infected ae. aegypti relative to uninfected competitors. although wolbachia infections impose fitness costs on the adult host, effects at the immature stages are less clear, particularly in competitive situations. we look f ...201424732463
air travel is associated with intracontinental spread of dengue virus serotypes 1-3 in brazil.dengue virus and its four serotypes (denv-1 to denv-4) infect 390 million people and are implicated in at least 25,000 deaths annually, with the largest disease burden in tropical and subtropical regions. we investigated the spatial dynamics of denv-1, denv-2 and denv-3 in brazil by applying a statistical framework to complete genome sequences. for all three serotypes, we estimated that the introduction of new lineages occurred within 7 to 10-year intervals. new lineages were most likely to be i ...201424743730
winter refuge for aedes aegypti and ae. albopictus mosquitoes in hanoi during winter.dengue occurs throughout the year in hanoi, vietnam, despite winter low temperatures <10°c. during july 2010 to march 2012, we surveyed monthly for aedes larvae and pupae in 120 houses in 8 hanoi districts. aedes albopictus preferred discarded containers in summer and pupal density drastically decreased in winter. aedes aegypti preferred concrete tanks and this preference increased in winter. even in winter, the lowest water temperature found in concrete tanks was >14°c, exceeding the developmen ...201424752230
survival and swimming behavior of insecticide-exposed larvae and pupae of the yellow fever mosquito aedes aegypti.the yellow fever mosquito aedes aegypti is essentially a container-inhabiting species that is closely associated with urban areas. this species is a vector of human pathogens, including dengue and yellow fever viruses, and its control is of paramount importance for disease prevention. insecticide use against mosquito juvenile stages (i.e. larvae and pupae) is growing in importance, particularly due to the ever-growing problems of resistance to adult-targeted insecticides and human safety concern ...201424761789
development and evaluation of a novel contamination device that targets multiple life-stages of aedes aegypti.the increasing global threat of dengue demands new and easily applicable vector control methods. ovitraps provide a low-tech and inexpensive means to combat dengue vectors. here we describe the development and optimization process of a novel contamination device that targets multiple life-stages of the aedes aegypti mosquito. special focus is directed to the diverse array of control agents deployed in this trap, covering adulticidal, larvicidal and autodissemination impacts.201424766772
dengue-associated kidney disease.a mosquito-borne viral illness highly prevalent in the tropics and subtropics, dengue is considered a major global health threat by the world health organization.201324772398
collagen-binding protein, aegyptin, regulates probing time and blood feeding success in the dengue vector mosquito, aedes aegypti.mosquito salivary glands have important roles in blood feeding and pathogen transmission. however, the biological relevance of many salivary components has yet to be determined. aegyptin, a secreted salivary protein from aedes aegypti, binds collagen and inhibits platelet aggregation and adhesion. we used a transgenic approach to study the relevance of aegyptin in mosquito blood feeding. aedes aegypti manipulated genetically to express gene-specific inverted-repeat rna sequences exhibited signif ...201424778255
apropos: 'preliminary evaluation on the efficiency of the kit platelia dengue ns1 ag-elisa to detect dengue virus in dried aedes aegypti: a potential tool to improve dengue surveillance'.only simple, point-of-care, assay formats of the platellia dengue ns1 ag-elisa would be suitable to identify dengue virus in aedes aegypti mosquitoes in dengue-endemic areas lacking sophisticated laboratory infrastructure and trained laboratory personnel.201424779373
[the incorporation of activities to control dengue by community health agents].to evaluate the performance of community health agents when dengue control activities were added to their tasks.024789644
[dengue-related knowledge, attitudes, and practices in primary schools in tapachula, chiapas, mexico].to identify dengue-related knowledge, attitudes, and practices among primary school students in tapachula, chiapas, mexico, before and after an educational intervention.201424793869
genetic shifting: a novel approach for controlling vector-borne diseases.rendering populations of vectors of diseases incapable of transmitting pathogens through genetic methods has long been a goal of vector geneticists. we outline a method to achieve this goal that does not involve the introduction of any new genetic variants to the target population. rather we propose that shifting the frequencies of naturally occurring alleles that confer refractoriness to transmission can reduce transmission below a sustainable level. the program employs methods successfully use ...201424794113
spatio-temporal dynamics of dengue 2009 outbreak in córdoba city, argentina.during 2009 the biggest dengue epidemic to date occurred in argentina, affecting almost half the country. we studied the spatio-temporal dynamics of the outbreak in the second most populated city of the country, córdoba city. confirmed cases and the results of an aedes aegypti monitoring during the outbreak were geolocated. the imported cases began in january, and the autochthonous in march. thirty-three percent of the 130 confirmed cases were imported, and occurred mainly at the center of the c ...201424795212
multi-scale analysis of the associations among egg, larval and pupal surveys and the presence and abundance of adult female aedes aegypti (stegomyia aegypti) in the city of merida, mexico.despite decades of research, there is still no agreement on which indices of aedes aegypti (stegomyia aegypti) (diptera: culicidae) presence and abundance better quantify entomological risk for dengue. this study reports the results of a multi-scale, cross-sectional entomological survey carried out in 1160 households in the city of merida, mexico to establish: (a) the correlation between levels of ae. aegypti presence and abundance detected with aspirators and ovitraps; (b) which immature and eg ...201424797405
wolbachia infection does not alter attraction of the mosquito aedes (stegomyia) aegypti to human odours.the insect endosymbiont wolbachia pipientis (rickettsiales: rickettsiaceae) is undergoing field trials around the world to determine if it can reduce transmission of dengue virus from the mosquito stegomyia aegypti to humans. two different wolbachia strains have been released to date. the primary effect of the wmel strain is pathogen protection whereby infection with the symbiont limits replication of dengue virus inside the mosquito. a second strain, wmelpop, induces pathogen protection, reduce ...201424797695
influence of mosquito genotype on transcriptional response to dengue virus infection.the mosquito aedes aegypti is the principal vector that transmits dengue virus (denv) to humans. the primary factors that trigger a susceptible or refractory interaction of a. aegypti with denv are not well understood. in this study, our aim is to characterize the influence of vector genotype on differential gene expression of susceptible vs. refractory a. aegypti strains to denv infection. to accomplish that, we identified differential expression of a set of complementary dnas (cdnas; n = 9,504 ...201424798794
geometric morphometrics of nine field isolates of aedes aegypti with different resistance levels to lambda-cyhalothrin and relative fitness of one artificially selected for resistance.aedes aegypti, a mosquito closely associated with humans, is the principal vector of dengue virus which currently infects about 400 million people worldwide. because there is no way to prevent infection, public health policies focus on vector control; but insecticide-resistance threatens them. however, most insecticide-resistant mosquito populations exhibit fitness costs in absence of insecticides, although these costs vary. research on components of fitness that vary with insecticide-resistance ...201424801598
comparing dengue and chikungunya emergence and endemic transmission in a. aegypti and a. albopictus.chikungunya and dengue are re-emerging mosquito-borne infectious diseases that are of increasing concern as human travel and expanding mosquito ranges increase the risk of spread. we seek to understand the differences in transient and endemic behavior of chikungunya and dengue; risk of emergence for different virus-vector assemblages; and the role that virus evolution plays in disease dynamics and risk. to address these questions, we adapt a mathematical mosquito-borne disease model to chikungun ...201424801860
chemical composition and larvicidal activity of plant extracts from clausena dentata (willd) (rutaceae) against dengue, malaria, and filariasis vectors.mosquitoes in the larval stage are attractive targets for pesticides because mosquitoes breed in water, and thus, it is easy to deal with them in this habitat. the use of conventional pesticides in the water sources, however, introduces many risks to people and/or the environment. natural pesticides, especially those derived from plants, are more promising in this aspect. aromatic plants and their essential oils are very important sources of many compounds that are used in different respects. in ...201424802866
field evaluation of the response of aedes albopictus (stegomyia albopicta) to three oviposition attractants and different ovitrap placements using black and clear autocidal ovitraps in a rural area of same, timor-leste.known oviposition attractants or stimulants were compared, singly and in combination, using inexpensive autocidal ovitraps designed to trap emerging adults, in a rural area of timor-leste during the dry season. in this area, the dengue vector aedes albopictus (stegomyia albopicta) skuse (diptera: culicidae) was abundant, but aedes aegypti (stegomyia aegypti) l. was not detected. the attractants were: (a) a compound found in aedes eggs (dodecanoic acid); (b) components of nitrogen, phosphorous an ...201424805793
fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse aedes aegypti strain.in 2006, we reported a mariner (mos1)-transformed aedes aegypti line, carb77, which was highly resistant to dengue-2 virus (denv2). carb77 mosquitoes expressed a denv2-specific inverted-repeat (ir) rna in midgut epithelial cells after ingesting an infectious bloodmeal. the ir-rna formed double-stranded denv2-derived rna, initiating an intracellular antiviral rna interference (rnai) response. however, carb77 mosquitoes stopped expressing the ir-rna after 17 generations in culture and lost their d ...201424810399
determining the spatial autocorrelation of dengue vector populations: influences of mosquito sampling method, covariables, and vector control.we investigated spatial autocorrelation of female aedes aegypti l. mosquito abundance from bg-sentinel trap and sticky ovitrap collections in cairns, north queensland, australia. bg-sentinel trap collections in 2010 show a significant spatial autocorrelation across the study site and over a smaller spatial extent, while sticky ovitrap collections only indicate a non-significant, weak spatial autocorrelation. the bg-sentinel trap collections were suitable for spatial interpolation using ordinary ...201424820568
the 2014 fifa world cup: communicable disease risks and advice for visitors to brazil--a review from the latin american society for travel medicine (slamvi).the next fifa world cup will be held in brazil in june-july 2014. around 600,000 international visitors and participants (as well over 3 million domestic travelers) are expected. this event will take place in twelve cities. this event poses specific challenges, given its size and the diversity of attendees, including the potential for the transmission of imported or endemic communicable diseases, especially those that have an increased transmission rate as a result of close human proximity, eg, ...201424821081
reduced survival and reproductive success generates selection pressure for the dengue mosquito aedes aegypti to evolve resistance against infection by the microsporidian parasite vavraia culicis.the success and sustainability of control measures aimed at reducing the transmission of mosquito-borne diseases will depend on how they influence the fitness of mosquitoes in targeted populations. we investigated the effects of the microsporidian parasite vavraia culicis on the survival, blood-feeding behaviour and reproductive success of female aedes aegypti mosquitoes, the main vector of dengue. infection reduced survival to adulthood and increased adult female mosquito age-dependent mortalit ...201424822081
spatial distribution of the risk of dengue and the entomological indicators in sumaré, state of são paulo, brazil.dengue fever is a major public health problem worldwide, caused by any of four virus (denv-1, denv-2, denv-3 and denv-4; flaviviridae: flavivirus), transmitted by aedes aegypti mosquito. reducing the levels of infestation by a. aegypti is one of the few current strategies to control dengue fever. entomological indicators are used by dengue national control program to measure the infestation of a. aegypti, but little is known about predictive power of these indicators to measure dengue risk. in t ...201424831806
characterization of the spatial and temporal dynamics of the dengue vector population established in urban areas of fernando de noronha, a brazilian oceanic island.aedes aegypti has played a major role in the dramatic expansion of dengue worldwide. the failure of control programs in reducing the rhythm of global dengue expansion through vector control suggests the need for studies to support more appropriated control strategies. we report here the results of a longitudinal study on ae. aegypti population dynamics through continuous egg sampling aiming to characterize the infestation of urban areas of a brazilian oceanic island, fernando de noronha. the spa ...201424832009
bicluster pattern of codon context usages between flavivirus and vector mosquito aedes aegypti: relevance to infection and transcriptional response of mosquito genes.the mosquito aedes aegypti is the primary vector of dengue virus (denv) infection in most of the subtropical and tropical countries. besides denv, yellow fever virus (yfv) is also transmitted by a. aegypti. susceptibility of a. aegypti to west nile virus (wnv) has also been confirmed. although studies have indicated correlation of codon bias between flaviviridae and their animal/insect hosts, it is not clear if codon sequences have any relation to susceptibility of a. aegypti to denv, yfv and wn ...201424838953
vertical transmission of key west dengue-1 virus by aedes aegypti and aedes albopictus (diptera: culicidae) mosquitoes from florida.following the 2009 and 2010 dengue-1 (denv-1) outbreaks in key west, fl, we used florida aedes aegypti (l.) mosquitoes and denv-1 isolated from key west in 2010 to test the hypothesis that if the 2009 and 2010 denv-1 genome sequences are similar, then vertical transmission of denv-1 from infected ae. aegypti female mosquitoes to their eggs could have served as an interepidemic reservoir between outbreaks. we also investigated the ability of florida aedes albopictus (skuse) mosquitoes to vertical ...024843934
designing a community engagement framework for a new dengue control method: a case study from central vietnam.the wolbachia strategy aims to manipulate mosquito populations to make them incapable of transmitting dengue viruses between people. to test its efficacy, this strategy requires field trials. public consultation and engagement are recognized as critical to the future success of these programs, but questions remain regarding how to proceed. this paper reports on a case study where social research was used to design a community engagement framework for a new dengue control method, at a potential r ...201424853391
myco-synthesis of silver nanoparticles using beauveria bassiana against dengue vector, aedes aegypti (diptera: culicidae).the efficacy of silver synthesized biolarvicide with the help of entomopathogenic fungus, beauveria bassiana, was assessed against the different larval instars of dengue vector, aedes aegypti. the silver nanoparticles were observed and characterized by a scanning electron microscope (sem) and energy-dispersive x-ray (edx). a surface plasmon resonance band was observed at 420 nm in uv-vis spectrophotometer. the characterization was confirmed by shape (spherical), size 36.88-60.93 nm, and edx spec ...201424861012
bacteria as a source of oviposition attractant for aedes aegypti mosquitoes.since a safe and effective mass vaccination program against dengue fever is not presently available, a good way to prevent and control dengue outbreaks depends mainly on controlling the mosquito vectors. aedes aegypti mosquito populations can be monitored and reduced by using ovitraps baited with organic infusions. a series of laboratory experiments were conducted which demonstrated that the bacteria in bamboo leaf infusion produce volatile attractants and contact chemical stimulants attractive ...201424862053
large indoor cage study of the suppression of stable aedes aegypti populations by the release of thiotepa-sterilised males.the sterile insect technique (sit) is a promising pest control method in terms of efficacy and environmental compatibility. in this study, we determined the efficacy of thiotepa-sterilised males in reducing the target aedes aegypti populations. treated male pupae were released weekly into large laboratory cages at a constant ratio of either 5:1 or 2:1 sterile-to-fertile males. a two-to-one release ratio reduced the hatch rate of eggs laid in the cage by approximately a third and reduced the adul ...201424863972
risk assessment of flavivirus transmission in namibia.the role of arboviruses causing acute febrile illness in sub-saharan africa is receiving more attention. reports of dengue in tourists were published nearly 10 years ago in namibia, but the current epidemiology of arboviruses is unknown and surveys of mosquito vectors have not been carried out since the 1950s. to begin addressing this knowledge gap, a prospective cross-sectional study was conducted using samples from volunteer blood donors linked to questionnaire. serum samples were tested using ...201424865792
infection with dengue-2 virus alters proteins in naturally expectorated saliva of aedes aegypti mosquitoes.dengue virus (denv) is responsible for up to approximately 300 million infections and an increasing number of deaths related to severe manifestations each year in affected countries throughout the tropics. it is critical to understand the drivers of this emergence, including the role of vector-virus interactions. when a denv-infected aedes aegypti mosquito bites a vertebrate, the virus is deposited along with a complex mixture of salivary proteins. however, the influence of a denv infection upon ...201424886023
survival of larvivorous fish used for biological control of aedes aegypti (diptera: culicidae) combined with different larvicides.to evaluate combinations of larvicides and fish which are predators of larvae of aedes aegypti, namely betta splendens, trichogaster trichopterus and poecilia reticulata.201424890120
[dengue vector control using ether fractions from two plants (asteraceae) as larvicide].evaluating the larvicidal activity of two ether factions from asteraceae (the aster, daisy or sunflower family, i.e. heli opsisoppositifolia (l.) druce (oxeye, sunflower-like) and jaegeria hirta (lag.) less (weed-like)) on aedes (stegomyia) aegypti (l.) final third instar or initial fourth instar larvae near the town of armenia in the quindío department in colombia.201424892666
climate change and the potential global distribution of aedes aegypti: spatial modelling using gis and climex.we examined the potential added risk posed by global climate change on the dengue vector aedes aegypti abundance using climex, a powerful tool for exploring the relationship between the fundamental and realised niche of any species. after calibrating the model using data from several knowledge domains, including geographical distribution records, we estimated potential distributions of the mosquito under current and future potential scenarios. the impact of climate change on its potential distri ...201424893017
developing exon-primed intron-crossing (epic) markers for population genetic studies in three aedes disease vectors.aedes aegypti, aedes notoscriptus, and aedes albopictus are important vectors of many arboviruses implicated in human disease such as dengue fever. genetic markers applied across vector species can provide important information on population structure, gene flow, insecticide resistance, and taxonomy, however, robust microsatellite markers have proven difficult to develop in these species and mosquitoes generally. here we consider the utility and transferability of 15 ribosome protein (rp) exon-p ...201524895297
the impact of temperature on the bionomics of aedes (stegomyia) aegypti, with special reference to the cool geographic range margins.the mosquito aedes (stegomyia) aegypti (l.), which occurs widely in the subtropics and tropics, is the primary urban vector of dengue and yellow fever viruses, and an important vector of chikungunya virus. there is substantial interest in how climate change may impact the bionomics and pathogen transmission potential of this mosquito. this forum article focuses specifically on the effects of temperature on the bionomics of ae. aegypti, with special emphasis on the cool geographic range margins w ...201424897844
a spatial model with pulsed releases to compare strategies for the sterile insect technique applied to the mosquito aedes aegypti.we present a simple mathematical model to replicate the key features of the sterile insect technique (sit) for controlling pest species, with particular reference to the mosquito aedes aegypti, the main vector of dengue fever. the model differs from the majority of those studied previously in that it is simultaneously spatially explicit and involves pulsed, rather than continuous, sterile insect releases. the spatially uniform equilibria of the model are identified and analysed. simulations are ...201424929226
mechanisms of pyrethroid resistance in the dengue mosquito vector, aedes aegypti: target site insensitivity, penetration, and metabolism.aedes aegypti is the major vector of yellow and dengue fevers. after 10 generations of adult selection, an a. aegypti strain (sp) developed 1650-fold resistance to permethrin, which is one of the most widely used pyrethroid insecticides for mosquito control. sp larvae also developed 8790-fold resistance following selection of the adults. prior to the selections, the frequencies of v1016g and f1534c mutations in domains ii and iii, respectively, of voltage-sensitive sodium channel (vssc, the targ ...201424945250
insecticidal compounds from rhizophoraceae mangrove plants for the management of dengue vector aedes aegypti.mosquito control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of botanical origin could serve as potential alternatives in future. larvicidal efficacies of different parts of mangrove plants belonging to rhizophoraceae family were tested against the late iv instar larvae of dengue vector, aedes aegypti.201424947217
properties for sourcing nigerian larvicidal plants.aedes aegypti is the primary vector of chikungunya, yellow and dengue fevers. dengue fever is the major cause of child morbidity and hospitalisation in some asian and african countries, while yellow fever is prevalent in nigeria. the development of resistance to the available insecticides has necessitated the continued search for safer ones from plants. eighteen plant extracts with ethnomedical claims of or demonstrated febrifuge, antimalarial, insecticidal and insect repellent biological activi ...201424950440
pharmacological validation of an inward-rectifier potassium (kir) channel as an insecticide target in the yellow fever mosquito aedes aegypti.mosquitoes are important disease vectors that transmit a wide variety of pathogens to humans, including those that cause malaria and dengue fever. insecticides have traditionally been deployed to control populations of disease-causing mosquitoes, but the emergence of insecticide resistance has severely limited the number of active compounds that are used against mosquitoes. thus, to improve the control of resistant mosquitoes there is a need to identify new insecticide targets and active compoun ...201424959745
ecobiosocial community intervention for improved aedes aegypti control using water container covers to prevent dengue: lessons learned from girardot colombia. 201424962850
application of quantitative pcr for quantization of densovirus genome.densovirus is classified as invertebrate virus belonging to the subfamily densovirinae of parvoviridae family. this group of viruses infects only insects and several densoviruses have been isolated from indigenous mosquitoes and mosquito cell lines. a number of mosquitoes, especially aedes aegypti and ae. albopictus are important vectors of viruses, which are the major causes of dengue hemorrhagic fever and yellow fever in humans. as densoviruses do not cause any pathology in humans, these virus ...201424964653
using garp to predict the range of aedes aegypti in china.dengue fever and dengue hemorrhagic fever are common mosquito-borne diseases in tropical and subtropical regions, and are mainly transmitted by the mosquito aedes aegypti (diptera: culicidae). the international trade of used tires, coupled with its anthropophilic habit, has enabled ae. aegypti to colonise new areas in china. we used genetic algorithum rule-set production (garp) to predict the putative current distribution of ae. aegypti based on data on its distribution 20 years ago and compared ...201424968668
clinical and virological descriptive study in the 2011 outbreak of dengue in the amazonas, brazil.dengue is a vector-borne disease in the tropical and subtropical region of the world and is transmitted by the mosquito aedes aegypti. in the state of amazonas, brazil during the 2011 outbreak of dengue all the four dengue virus (denv) serotypes circulating simultaneously were observed. the aim of the study was to describe the clinical epidemiology of dengue in manaus, the capital city of the state of the amazonas, where all the four denv serotypes were co-circulating simultaneously.201424978469
insecticide resistance in disease vectors from mayotte: an opportunity for integrated vector management.mayotte, a small island in the indian ocean, has been affected for many years by vector-borne diseases. malaria, bancroftian filariasis, dengue, chikungunya and rift valley fever have circulated or still circulate on the island. they are all transmitted by culicidae mosquitoes. to limit the impact of these diseases on human health, vector control has been implemented for more than 60 years on mayotte. in this study, we assessed the resistance levels of four major vector species (anopheles gambia ...201424984704
human to mosquito transmission of dengue viruses.the successful transmission of dengue virus from a human host to a mosquito vector requires a complex set of factors to align. it is becoming increasingly important to improve our understanding of the parameters that shape the human to mosquito component of the transmission cycle so that vaccines and therapeutic antivirals can be fully evaluated and epidemiological models refined. here we describe these factors, and discuss the biological and environmental impacts and demographic changes that ar ...201424987394
effects of insemination and blood-feeding on locomotor activity of aedes albopictus and aedes aegypti (diptera: culicidae) females under laboratory conditions.dengue is an arbovirus disease transmitted by two aedes mosquitoes: ae. aegypti and ae. albopictus. virgin females of these two species generally show a bimodal and diurnal pattern of activity, with early morning and late afternoon peaks. although some studies on the flight activity of virgin, inseminated and blood-fed ae. aegypti females have been carried out under laboratory conditions, little is known about the effects of such physiological states on the locomotor activity of ae. albopictus a ...201424990394
lineage ii of southeast asian/american denv-2 is associated with a severe dengue outbreak in the peruvian amazon.during 2010 and 2011, the loreto region of peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. this outbreak coincided with the reappearance of dengue virus-2 (denv-2) in loreto after almost 8 years. whole-genome sequence indicated that denv-2 from the outbreak belonged to lineage ii of the southeast asian/american genotype and was most closely related to viruses circulating in brazil during 2007 and 2008, whereas denv-2 previously circulating in loreto gro ...201425002298
assessing dengue infection risk in the southern region of taiwan: implications for control.dengue, one of the most important mosquito-borne diseases, is a major international public health concern. this study aimed to assess potential dengue infection risk from aedes aegypti in kaohsiung and the implications for vector control. here we investigated the impact of dengue transmission on human infection risk using a well-established dengue-mosquito-human transmission dynamics model. a basic reproduction number (r 0)-based probabilistic risk model was also developed to estimate dengue inf ...201525007831
insecticide resistance status of united states populations of aedes albopictus and mechanisms involved.aedes albopictus (skuse) is an invasive mosquito that has become an important vector of chikungunya and dengue viruses. immature ae. albopictus thrive in backyard household containers that require treatment with larvicides and when adult populations reach pest levels or disease transmission is ongoing, adulticiding is often required. to assess the feasibility of control of usa populations, we tested the susceptibility of ae. albopictus to chemicals representing the main insecticide classes with ...201425013910
distinct variation in vector competence among nine field populations of aedes aegypti from a brazilian dengue-endemic risk city.in brazil, dengue epidemics erupt sporadically throughout the country and it is unclear if outbreaks may initiate a sustainable transmission cycle. there are few studies evaluating the ability of brazilian aedes aegypti populations to transmit dengue virus (denv). the aim of this study was to compare denv susceptibility of field-captured ae. aegypti populations from nine distinct geographic areas of the city of belo horizonte in 2009 and 2011. infection rate (ir), vector competence (vc) and diss ...201425015526
effect of niloticin, a protolimonoid isolated from limonia acidissima l. (rutaceae) on the immature stages of dengue vector aedes aegypti l. (diptera: culicidae).the aim of the present study was to evaluate the mosquitocidal activity of fractions and a compound niloticin from the hexane extract of limonia acidissima l. leaves on eggs, larvae and pupae of aedes aegypti l. (diptera: culicidae). in these bioassays, the eggs, larvae and pupae were exposed to concentrations of 2.5, 5.0, 7.5 and 10.0ppm for fractions and 0.5, 1.0, 1.5 and 2.0ppm for compound. after 24h, the mortality was assessed and the lc50 and lc90 values were calculated for larvae and pupa ...201425019220
differential replication of dengue virus serotypes 2 and 3 in coinfections of c6/36 cells and aedes aegypti mosquitoes.different dengue virus (denv) serotypes have been associated with greater epidemic potential. in turn, the increased frequency in cases of severe forms of dengue has been associated with the cocirculation of several serotypes. because colombia is a country with an endemic presence of all four denv serotypes, the aim of this study was to evaluate the in vivo and in vitro replication of the denv-2 and denv-3 strains under individual infection and coinfection conditions.201425022298
role of semaphorin-1a in the developing visual system of the disease vector mosquito aedes aegypti.despite the devastating impact of mosquito-borne illnesses on human health, very little is known about mosquito developmental biology, including development of the mosquito visual system. mosquitoes possess functional adult compound eyes as larvae, a trait that makes them an interesting model in which to study comparative developmental genetics. here, we functionally characterize visual system development in the dengue and yellow fever vector mosquito aedes aegypti, in which we use chitosan/sirn ...201425045063
global temperature constraints on aedes aegypti and ae. albopictus persistence and competence for dengue virus transmission.dengue is a disease that has undergone significant expansion over the past hundred years. understanding what factors limit the distribution of transmission can be used to predict current and future limits to further dengue expansion. while not the only factor, temperature plays an important role in defining these limits. previous attempts to analyse the effect of temperature on the geographic distribution of dengue have not considered its dynamic intra-annual and diurnal change and its cumulativ ...201425052008
evaluation of seed extracts from plants found in the caatinga biome for the control of aedes aegypti.dengue fever, currently the most important arbovirus, is transmitted by the bite of the aedes aegypti mosquito. given the absence of a prophylactic vaccine, the disease can only be controlled by combating the vector insect. however, increasing reports of resistance and environmental damage caused by insecticides have led to the urgent search for new safer alternatives. in this regard, plants stand out as a source of easy-to-obtain biodegradable insecticide molecules. twenty (20) plant seed extra ...201425056942
co-circulation of dengue and chikungunya viruses, al hudaydah, yemen, 2012.we investigated 400 cases of dengue-like illness in persons hospitalized during an outbreak in al hudaydah, yemen, in 2012. overall, 116 dengue and 49 chikungunya cases were diagnosed. dengue virus type 2 was the predominant serotype. the co-circulation of these viruses indicates that mosquitoborne infections represent a public health threat in yemen.025061762
origin of the dengue fever mosquito, aedes aegypti, in california.dengue fever is among the most widespread vector-borne infectious diseases. the primary vector of dengue is the aedes aegypti mosquito. ae. aegypti is prevalent in the tropics and sub-tropics and is closely associated with human habitats outside its native range of africa. while long established in the southeastern united states of america where dengue is re-emerging, breeding populations have never been reported from california until the summer of 2013. using 12 highly variable microsatellite l ...201425077804
mating competitiveness and life-table comparisons between transgenic and indian wild-type aedes aegypti l.ox513a is a genetically engineered strain of aedes aegypti carrying a repressible, dominantly inherited transgene that confers lethality in immature heterozygous progeny. released male ox513a adults have proven to be effective for the localised suppression of wild ae. aegypti, highlighting its potential in vector control. mating and life-table assessments were used to compare ox513a with reared ae. aegypti strains collected from new delhi and aurangabad regions in india.201425078081
spatio-temporal distribution of dengue and lymphatic filariasis vectors along an altitudinal transect in central nepal.rapidly increasing temperatures in the mountain region of nepal and recent reports of dengue fever and lymphatic filariasis cases from mountainous areas of central nepal prompted us to study the spatio-temporal distribution of the vectors of these two diseases along an altitudinal transect in central nepal.201425078276
[dengue fever in mainland france].dengue fever is the most widespread distributed vector borne viral disease. it is transmitted through the bites of aedes aegypti and aedes albopictus mosquitoes. with the expansion of aedes albopictus and increasing travel exchange, it is no longer limited to the tropical zone and transmission has been documented in temperate areas. in mainland france, where aedes albopictus has been present and disseminating since 2004, 2 episodes of autochthonous transmission occurred in 2010 and in 2013. cont ...201425080833
patterns of geographic expansion of aedes aegypti in the peruvian amazon.in the peruvian amazon, the dengue vector aedes aegypti is abundant in large urban centers such as iquitos. in recent years, it has also been found in a number of neighboring rural communities with similar climatic and socioeconomic conditions. to better understand ae. aegypti spread, we compared characteristics of communities, houses, and containers in infested and uninfested communities.201425101786
suppressing dengue-2 infection by chemical inhibition of aedes aegypti host factors.dengue virus host factors (denv hfs) that are essential for the completion of the infection cycle in the mosquito vector and vertebrate host represent potent targets for transmission blocking. here we investigated whether known mammalian denv hf inhibitors could influence virus infection in the arthropod vector a. aegypti. we evaluated the potency of bafilomycin (baf; inhibitor of vacuolar h+-atpase (vatpase)), mycophenolic acid (mpa; inhibitor of inosine-5'-monophosphate dehydrogenase (impdh)), ...201425101828
shifting patterns of aedes aegypti fine scale spatial clustering in iquitos, peru.empiric evidence shows that aedes aegypti abundance is spatially heterogeneous and that some areas and larval habitats produce more mosquitoes than others. there is a knowledge gap, however, with regards to the temporal persistence of such ae. aegypti abundance hotspots. in this study, we used a longitudinal entomologic dataset from the city of iquitos, peru, to (1) quantify the spatial clustering patterns of adult ae. aegypti and pupae counts per house, (2) determine overlap between clusters, ( ...201425102062
heterogeneous feeding patterns of the dengue vector, aedes aegypti, on individual human hosts in rural thailand.mosquito biting frequency and how bites are distributed among different people can have significant epidemiologic effects. an improved understanding of mosquito vector-human interactions would refine knowledge of the entomological processes supporting pathogen transmission and could reveal targets for minimizing risk and breaking pathogen transmission cycles.201425102306
biological control of mosquitoes in scrap tires in brownsville, texas, usa and matamoros, tamaulipas, mexico.dengue periodically circulates in southern texas and neighboring tamaulipas, mexico; thus, a closer examination of human and vector ecology at the northern limits of north american transmission may improve prevention activities. scrap tires produce large mosquito populations and increase the risk of dengue transmission. some households choose not to pay tire disposal fees, and many tires are illegally dumped in residential areas. biological control may provide low-cost and environmentally friend ...201425102598
natural vertical transmission of dengue-1 virus in aedes aegypti populations in acapulco, mexico.we carried out dengue virus surveillance in aedes aegypti populations from 47 neighborhoods of acapulco during the rainy season of 2011 following a standard national protocol and as an improvement of the entomological surveillance of the mexican dengue control program. a total of 4,146 ae. aegypti adults collected indoors and/or emerged from eggs, larvae, or pupae from households with dengue reports (probable or confirmed cases), were grouped into pools and processed using a standardized serotyp ...201425102601
synthesis, depletion and cell-type expression of a protein from the male accessory glands of the dengue vector mosquito aedes aegypti.aedes aegypti males transfer sperm and seminal fluid proteins (sfps), primarily produced by male accessory glands (ags), to females during mating. when collectively injected or transplanted into females, ag tissues and/or seminal fluid homogenates have profound effects on aedes female physiology and behavior. to identify targets and design new strategies for vector control, it is important to understand the biology of the ags. thus, we examined characteristics of ag secretion and development in ...201425107876
laboratory surveillance of dengue in rio grande do sul, brazil, from 2007 to 2013.according to official records, dengue was introduced in brazil in the 80's; since then several epidemics have occurred. meanwhile, in rio grande do sul (rs, southern brazil) the first autochthonous case occurred only in 2007.201425116186
exploring new thermal fog and ultra-low volume technologies to improve indoor control of the dengue vector, aedes aegypti (diptera: culicidae).control of the mosquito vector, aedes aegypti (l.), inside human habitations must be performed quickly and efficiently to reduce the risk of transmission during dengue outbreaks. as part of abroad study to assess the efficacy of dengue vector control tools for the u.s. military, two pesticide delivery systems (ultra-low volume [ulv] and thermal fog) were evaluated for their ability to provide immediate control of ae. aegypti mosquitoes with a contact insecticide inside simulated urban structures ...201425118418
feeding host range of aedes albopictus (diptera: culicidae) demonstrates its opportunistic host-seeking behavior in rural singapore.aedes albopictus (skuse) is a competent vector of arboviruses of public health importance, including dengue virus (denv) and chikungunya virus viruses. ae. albopictus is the primary vector of chikungunya virus in singapore. however, despite being ubiquitous, it plays a secondary role in denv transmission. the vectorial capacity of ae. albopictus for denv in field settings appears to be weak because dengue primarily occurs in aedes aegypti (l.)-dominated, urban settings of the country. as host-se ...201425118424
temporal correlations between mosquito-based dengue virus surveillance measures or indoor mosquito abundance and dengue case numbers in mérida city, méxico.surveillance of dengue virus (denv) in aedes (stegomyia) aegypti (l.) females is of potential interest because human denv infections are commonly asymptomatic, which decreases the effectiveness of dengue case surveillance to provide early warning of building outbreaks. our primary aim was to examine if mosquito-based virological measures--monthly percentages of examined ae. aegypti females infected with denv or examined homes from which at least one denv-infected ae. aegypti female was collected ...025118425
aedes aegypti larvicide from the ethanolic extract of piper nigrum black peppercorns.due to unavailability of a vaccine and a specific cure to dengue, the focus nowadays is to develop an effective vector control method against the female aedes aegypti mosquito. this study aims to determine the larvicidal fractions from piper nigrum ethanolic extracts (pnpcme) and to elucidate the identity of the bioactive compounds that comprise these larvicidal fractions. larvicidal assay was performed by subjecting 3rd to 4th a. aegypti instar larvae to pnpcme of p. nigrum. the pnpcme exhibite ...201525118563
oral susceptibility of aedes aegypti (diptera: culicidae) from senegal for dengue serotypes 1 and 3 viruses.to investigate the potential for domestic and wild populations of aedes aegypti from dakar and kedougou to develop a disseminated infection after exposure to denv-3 and denv-1.201425134541
coexistence of philodina roseola (rotifera: bdelloidea) with larvae of aedes aegypti in india.the vector mosquitoes, aedes aegypti and aedes albopictus of dengue and chikungunya fever are closely associated with human habitations and adapted to feed on human blood. they undergo larval and pupal development in natural and artificial freshwater collections in the urban and peri-urban environment. although reports are available about the feeding behaviour of the thriving mosquito larvae, much information is still required to understand the successful survival of aedes mosquitoes in small an ...201425134889
larvicidal efficacy screening of anacardaciae crude extracts on the dengue hemorrhagic vector, aedes aegypti.vector-borne diseases are still rife because of the re-emergence of diseases transmitted by mosquitoes. the objective of this paper is to evaluate the larvicidal efficacy of crude leaf extract of mangifera indica, gluta renghas, and melanochyla fasciculiflora against vector of dengue hemorrhagic fever, aedes aegypti. these plant species are endemic species and widely distributed in malaysian forests. leaves of ma. indica, g. renghas and m. fascculiflora were collected from teluk bahang national ...201425134898
epidemiology of dengue in a high-income country: a case study in queensland, australia.australia is one of the few high-income countries where dengue transmission regularly occurs. dengue is a major health threat in north queensland (nq), where the vector aedes aegypti is present. whether nq should be considered as a dengue endemic or epidemic region is an ongoing debate. to help address this issue, we analysed the characteristics of locally-acquired (la) and imported dengue cases in nq through time and space. we describe the epidemiology of dengue in nq from 1995 to 2011, to iden ...201425138897
adulticidal properties of synthesized silver nanoparticles using leaf extracts of feronia elephantum (rutaceae) against filariasis, malaria, and dengue vector mosquitoes.mosquito-borne diseases with an economic impact create loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates. mosquito control is facing a threat because of the emergence of resistance to synthetic insecticides. extracts from plants may be alternative sources of mosquito control agents because they constitute a rich source of bioactive compounds that are biodegradable into nontoxic products and potentially suitable for use to control mosquitoes. i ...201425146645
insecticide resistance and, efficacy of space spraying and larviciding in the control of dengue vectors aedes aegypti and aedes albopictus in sri lanka.unprecedented incidence of dengue has been recorded in sri lanka in recent times. source reduction and use of insecticides in space spraying/fogging and larviciding, are the primary means of controlling the vector mosquitoes aedes aegypti and ae. albopictus in the island nation. a study was carried out to understand insecticide cross-resistance spectra and mechanisms of insecticide resistance of both these vectors from six administrative districts, i.e. kandy, kurunegala, puttalam, gampaha, ratn ...201325149242
using wolbachia-based release for suppression of aedes mosquitoes: insights from genetic data and population simulations.a novel strategy for suppressing disease transmission by aedes aegypti, the main vector of dengue, uses releases of mosquitoes infected with the bacterium wolbachia pipientis. wolbachia are currently released to interfere with viral transmission, but there is also potential to use strains in mosquito suppression and elimination programs via the deleterious effects of the bacterium on the host. mosquito suppression depends on target areas being relatively isolated to prevent reinvasion and on loc ...201425154109
regulation of arginine methyltransferase 3 by a wolbachia-induced microrna in aedes aegypti and its effect on wolbachia and dengue virus replication.the gram-negative endosymbiotic bacteria, wolbachia, have been found to colonize a wide range of invertebrates, including over 40% of insect species. best known for host reproductive manipulations, some strains of wolbachia have been shown to reduce the host life span by about 50% and inhibit replication and transmission of dengue virus (denv) in the mosquito vector, aedes aegypti. the molecular mechanisms underlying these effects still are not well understood. our previous studies showed that w ...201425158106
aedesin: structure and antimicrobial activity against multidrug resistant bacterial strains.multidrug resistance, which is acquired by both gram-positive and gram-negative bacteria, causes infections that are associated with significant morbidity and mortality in many clinical settings around the world. because of the rapidly increasing incidence of pathogens that have become resistant to all or nearly all available antibiotics, there is a need for a new generation of antimicrobials with a broad therapeutic range for specific applications against infections. aedesin is a cecropin-like ...201425162372
insecticidal activity of leptodactylus knudseni and phyllomedusa vaillantii crude skin secretions against the mosquitoes anopheles darlingi and aedes aegypti.mosquitoes are important vectors of several diseases, including malaria and dengue, and control measures are mostly performed using chemical insecticides. unfortunately, mosquito resistance to commonly applied insecticides is widespread. therefore, a prospection for new molecules with insecticidal activity based on amazon biodiversity using the anurans leptodactylus knudseni and phyllomedusa vaillantii was performed against the mosquito species anopheles darlingi and aedes aegypti.201425165469
estimation of aedes aegypti (diptera: culicidae) population size and adult male survival in an urban area in panama.traditional mosquito control strategies rely heavily on the use of chemical insecticides. however, concerns about the efficiency of traditional control methods, environmental impact and emerging pesticide resistance have highlighted the necessity for developing innovative tools for mosquito control. some novel strategies, including release of insects carrying a dominant lethal gene (ridl®), rely on the sustained release of modified male mosquitoes and therefore benefit from a thorough understand ...201425165979
a single crossing-over event in voltage-sensitive na+ channel genes may cause critical failure of dengue mosquito control by insecticides.the voltage-sensitive sodium (na+) channel (vssc) is the target site of pyrethroid insecticides. pest insects develop resistance to this class of insecticide by acquisition of one or multiple amino acid substitution(s) in this channel. in southeast asia, two major vssc types confer pyrethroid resistance in the dengue mosquito vector aedes aegypti, namely, s989p+v1016g and f1534c. we expressed several types of vssc in xenopus oocytes and examined the effect of amino acid substitutions in vssc on ...201425166902
invasion of wolbachia at the residential block level is associated with local abundance of stegomyia aegypti, yellow fever mosquito, populations and property attributes.wolbachia can suppress dengue and control mosquito populations and this depends on the successful invasion of wolbachia-infected mosquitoes into local populations. ovitrap data collected during the recent invasion of wmel-infected stegomyia aegypti (diptera: culicidae) (linnaeus) into gordonvale near cairns, australia, were used to identify variables that help predict the success of localized invasion. based on the variance in wolbachia frequencies across gordonvale as well as at another release ...201425171611
dengue and dengue vectors in the who european region: past, present, and scenarios for the future.after 55 years of absence, dengue has re-emerged in the who european region both as locally transmitted sporadic cases and as an outbreak in madeira, driven by the introduction of people infected with the virus and the invasion of the vector mosquito species aedes aegypti and aedes albopictus. models predict a further spread of a albopictus, particularly under climate change conditions. dengue transmission models suggest a low risk in europe, but these models too rarely include transmission by a ...201425172160
aedes aegypti salivary protein "aegyptin" co-inoculation modulates dengue virus infection in the vertebrate host.dengue virus (denv) is transmitted in the saliva of the mosquito vector aedes aegypti during blood meal acquisition. this saliva is composed of numerous proteins with the capacity to disrupt hemostasis or modulate the vertebrate immune response. one such protein, termed "aegyptin," is an allergen and inhibitor of clot formation, and has been found in decreased abundance in the saliva of denv-infected mosquitoes. to examine the influence of aegyptin on denv infection of the vertebrate, we inocula ...201425173089
increased replicative fitness of a dengue virus 2 clade in native mosquitoes: potential contribution to a clade replacement event in nicaragua.the four dengue virus (denv) serotypes (denv serotype 1 [denv-1] to denv-4) are transmitted by aedes aegypti and a. albopictus mosquitoes, causing up to 390 million denv infections worldwide each year. we previously reported a clade replacement of the denv-2 asian-american genotype ni-1 clade by the ni-2b clade in managua, nicaragua. here, we describe our studies of the replicative ability of ni-1 and ni-2b viruses in an a. aegypti cell line (aag2) and a. aegypti mosquitoes reared from eggs coll ...201425187539
dynamics of midgut microflora and dengue virus impact on life history traits in aedes aegypti.significant morbidity and potential mortality following dengue virus infection is a re-emerging global health problem. due to the limited effectiveness of current disease control methods, mosquito biologists have been searching for new methods of controlling dengue transmission. while much effort has concentrated on determining genetic aspects to vector competence, paratransgenetic approaches could also uncover novel vector control strategies. the interactions of mosquito midgut microflora and p ...201425193134
[dengue fever in portuguese speaking countries: which epidemiological links may we set?].the recent occurrence of a number of outbreaks of dengue in portuguese speaking countries with no previous disease, aroused curiosity about the competing factors for the phenomenon and a need for better knowledge of the pathology. we review the dengue-related situation in portuguese speaking countries, linking the various outbreaks and trying to contribute to a better understanding of the phenomenon.201425203960
stability of the wmel wolbachia infection following invasion into aedes aegypti populations.the wmel infection of drosophila melanogaster was successfully transferred into aedes aegypti mosquitoes where it has the potential to suppress dengue and other arboviruses. the infection was subsequently spread into two natural populations at yorkeys knob and gordonvale near cairns, queensland in 2011. here we report on the stability of the infection following introduction and we characterize factors influencing the ongoing dynamics of the infection in these two populations. while the wolbachia ...201425211492
angola's 2013 dengue outbreak: clinical, laboratory and molecular analyses of cases from four portuguese institutions.dengue virus (denv) is the arbovirus with the widest impact on human health. in africa in general, and in angola in particular, the epidemiology and public health impact of denv is far from clear. however, rapid population growth, unplanned urbanization, increased international travel, and the presence of virus major vector (aedes aegypti) in the country suggest that denv transmission may occur.201425212088
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