Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| evaluation of the immunohistochemical expression of gal-1, gal-3 and gal-9 in the colon of chronic chagasic patients. | the aim of the present study was to evaluate the immunohistochemical expression of gal-1, gal-3 and gal-9 in the colon of chronic chagasic patients compared to biopsied non-chagasic patients. | 2017 | 28554765 |
| corrigendum: the contemporary distribution of trypanosoma cruzi infection in humans, alternative hosts and vectors. | this corrects the article doi: 10.1038/sdata.2017.50. | 2017 | 28556826 |
| the anti-protozoan drug nifurtimox preferentially inhibits clonogenic tumor cells under hypoxic conditions. | tumor hypoxia is an independent prognostic indicator of tumor malignant progression and poor patient survival. therefore, eradication of hypoxic tumor cells is of paramount importance for successful disease control. in this study, we have made a new discovery that nifurtimox, a clinically approved drug to treat chagas disease caused by the parasitic protozoan trypanosomes, can function as a hypoxia-activated cytotoxin. we have found that nifurtimox preferentially kill clonogenic tumor cells espe ... | 2017 | 28560059 |
| effects of astaxanthin in mice acutely infected with trypanosoma cruzi. | during trypanosoma cruzi infection, oxidative stress is considered a contributing factor for dilated cardiomyopathy development. in this study, the effects of astaxanthin (astx) were evaluated as an alternative drug treatment for chagas disease in a mouse model during the acute infection phase, given its anti-inflammatory, immunomodulating, and anti-oxidative properties. astx was tested in vitro in parasites grown axenically and in co-culture with vero cells. in vivo tests were performed in balb ... | 2017 | 28560955 |
| evaluation of antioxidant therapy in experimental chagas disease. | stimulation of inflammatory mediators such as cytokines and chemokines may cause oxidative stress in chagas disease. in this study, we evaluated the merit of vitamins c and e as antioxidant therapy to minimize the oxidative stress-induced damage in an experimental model of chagas disease. | 2017 | 28562754 |
| evaluating respiratory musculature, quality of life, anxiety, and depression among patients with indeterminate chronic chagas disease and symptoms of pulmonary hypertension. | chagas disease (cd) is progressive and incapacitating, especially when cardiopulmonary function is affected. for example, respiratory muscle weakness can cause dyspnea upon exertion and fatigue, which may be exacerbated when it is associated with pulmonary hypertension (ph). the present study aimed to evaluate respiratory musculature, quality of life, anxiety, and depression among patients with indeterminate chronic cd and symptoms of ph. | 2017 | 28562755 |
| chagas disease knowledge and risk behaviors of the homeless population in houston, tx. | chagas disease is a parasitic infection, caused by trypanosoma cruzi, endemic in latin america. sylvatic t. cruzi-infected triatomine vectors are present in rural and urban areas in the southern usa and may transmit t. cruzi infection to at-risk populations, such as homeless individuals. our study aimed to evaluate chagas disease knowledge and behaviors potentially associated with transmission risk of chagas disease among houston, texas' homeless population by performing interviews with 212 home ... | 2017 | 28567616 |
| isozyme variability in trypanosoma cruzi, the agent of chagas' disease: genetical, taxonomical, and epidemiological significance. | a genetic interpretation of the zymograms of 524 trypanosoma cruzi stocks from various hosts and representing a broad geographical range (united states to southern brazil) reveals high genetic variability (only one monomorphic locus out of 15) and suggests that this parasite has a diploid structure. the data do not give any indication of mendelian sexuality, although many opportunities are present for genetic exchange between extremely different genotypes. the population structure of t. cruzi ap ... | 1988 | 28567853 |
| moderate treadmill exercise training improves cardiovascular and nitrergic response and resistance to trypanosoma cruzi infection in mice. | there is evidence suggesting that exercise training (et) acts as a factor toward resistance to trypanosoma cruzi infection. however, the effects of mean arterial pressure (map), heart rate (hr), and nitric oxide (no) during the acute phase of infection has not been elucidated yet. swiss mice were randomly assigned into four groups: sedentary control (sc, n = 30), trained control (tc, n = 30), sedentary infected (si, n = 30), and trained infected (ti, n = 30). et was performed on the treadmill fo ... | 2017 | 28572772 |
| ergosterol isolated from the basidiomycete pleurotus salmoneostramineus affects trypanosoma cruzi plasma membrane and mitochondria. | major drawbacks of the available treatment against chagas disease (american trypanosomiasis) include its toxicity and therapeutic inefficiency in the chronic phase of the infection, which makes it a concern among neglected diseases. therefore, the discovery of alternative drugs for treating chronic chagas disease requires immediate action. in this work, we evaluated the mushroom pleurotus salmoneostramineus in the search for potential antiparasitic compounds. | 2017 | 28572816 |
| whole genome cardiac dna methylation fingerprint and gene expression analysis provide new insights in the pathogenesis of chronic chagas disease cardiomyopathy. | chagas disease, caused by the protozoan trypanosoma cruzi, is endemic in latin america and affects 10 million people worldwide. approximately 12,000 deaths attributable to chagas disease occur annually due to chagas disease cardiomyopathy (ccc), an inflammatory cardiomyopathy presenting with heart failure and arrythmia; 30% of infected subjects develop ccc years after infection. genetic mechanisms play a role in differential progression to ccc, but little is known about the role of epigenetic mo ... | 2017 | 28575239 |
| seroprevalence, cost per donation and reduction in blood supply due to positive and indeterminate results for infectious markers in a blood bank in lima, peru. | safety in transfusion medicine is subject to regulations and government legislation within a total quality framework. the aim of this study was to evaluate the impact of seroprevalence and indeterminate results on lost units and cost per donation. | 2017 | 28577645 |
| applicability of plant-based products in the treatment of trypanosoma cruzi and trypanosoma brucei infections: a systematic review of preclinical in vivo evidence. | chagas disease and sleeping sickness are neglected tropical diseases closely related to poverty, for which the development of plant-derived treatments has not been a promising prospect. thus, we systematicaly review the preclinical in vivo evidence on the applicability of plant-based products in the treatment of trypanosoma cruzi and trypanosoma brucei infections. characteristics such as disease models, treatments, toxicological safety and methodological bias were analysed. we recovered 66 full ... | 2017 | 28578742 |
| targeting cysteine proteases in trypanosomatid disease drug discovery. | chagas disease and human african trypanosomiasis are endemic conditions in latin america and africa, respectively, for which no effective and safe therapy is available. efforts in drug discovery have focused on several enzymes from these protozoans, among which cysteine proteases have been validated as molecular targets for pharmacological intervention. these enzymes are expressed during the entire life cycle of trypanosomatid parasites and are essential to many biological processes, including i ... | 2017 | 28579388 |
| mutations to cysteine residues in the trypanosoma cruzi b-cell superantigen tc24 diminish susceptibility to igm-mediated hydrolysis. | b-cell superantigens (bc-sags) are immunoevasins that have evolved in response to innate catalytic igm antibodies; germ-line encoded immunoglobulins present in the preimmune repertoire independent of prior antigen exposure. catalysis is the result of a 2-step process that involves the formation of a non-covalent bond between the bc-sag and the immunoglobulin first followed by covalent bond formation at the catalytic site resulting in target hydrolysis. tc24 is a recently described trypanosoma cr ... | 2017 | 28581897 |
| molecular diagnostics for chagas disease: up to date and novel methodologies. | chagas disease is caused by the parasite trypanosoma cruzi. it affects 7 million people, mainly in latin america. diagnosis is usually made serologically, but at some clinical scenarios serology cannot be used. then, molecular detection is required for early detection of congenital transmission, treatment response follow up, and diagnosis of immune-suppression reactivation. however, present tests are technically demanding and require well-equipped laboratories which make them unfeasible in low-r ... | 2017 | 28582629 |
| lamp-2 absence interferes with plasma membrane repair and decreases t. cruzi host cell invasion. | trypanosoma cruzi enters host cells by subverting the mechanism of cell membrane repair. in this process, the parasite induces small injuries in the host cell membrane leading to calcium entry and lysosomal exocytosis, which are followed by compensatory endocytosis events that drive parasites into host cells. we have previously shown that absence of both lamp-1 and 2, major components of lysosomal membranes, decreases invasion of t. cruzi into host cells, but the mechanism by which they interfer ... | 2017 | 28586379 |
| pheophorbide a, a compound isolated from the leaves of arrabidaea chica, induces photodynamic inactivation of trypanosoma cruzi. | approximately 6-7 million people are infected with trypanosoma cruzi, the etiological agent of chagas' disease. only two therapeutic compounds have been found to be useful against this disease: nifurtimox and benznidazole. these drugs have been effective in the acute phase of the disease but less effective in the chronic phase; they also have many side effects. thus, the search for new compounds with trypanocidal action is necessary. natural products can be the source of many important substance ... | 2017 | 28587855 |
| heme a synthesis and cco activity are essential for trypanosoma cruzi infectivity and replication. | trypanosoma cruzi, the causative agent of chagas disease, presents a complex life cycle and adapts its metabolism to nutrients' availability. although t. cruzi is an aerobic organism, it does not produce heme. this cofactor is acquired from the host and is distributed and inserted into different heme-proteins such as respiratory complexes in the parasite's mitochondrion. it has been proposed that t. cruzi's energy metabolism relies on a branched respiratory chain with a cytochrome c oxidase-type ... | 2017 | 28588043 |
| repurposing of human kinase inhibitors in neglected protozoan diseases. | human african trypanosomiasis (hat), chagas disease, and leishmaniasis belong to a group of infectious diseases known as neglected tropical diseases and are induced by infection with protozoan parasites named trypanosomatids. drugs in current use have several limitations, and therefore new candidate drugs are required. the majority of current therapeutic trypanosomatid targets are enzymes or cell-surface receptors. among these, eukaryotic protein kinases are a major group of protein targets whos ... | 2017 | 28590590 |
| entomological survey in the state of piauí, northeastern brazil, reveals intradomiciliary colonization of triatoma brasiliensis macromelasoma. | this survey aimed to assess the presence of triatomine vectors of chagas disease within the rural communities of são joão do piauí, northeast brazil. intradomiciliary and peridomiciliary collection strategies were implemented wherein 279 specimens of triatoma brasiliensis macromelasoma, both nymph and adult were found in 15 (50%) of the studied homes. of the intradomiciliary insects, 73 (67.6%) were identified as nymph instars (1st instar [n1]= 6, n2= 14, n3= 28, n4= 7 and n5= 18). in the studie ... | 2017 | 28591255 |
| prediction models for decision-making on chagas disease. | 2017 | 28591323 | |
| [strategy to improve access to etiological treatment of chagas disease at the first level of care in argentina]. | improve distribution of etiological treatment of chagas disease by identifying barriers to the decentralization of treatment to the first level of care in argentina. | 2017 | 28591327 |
| sexual transmission of american trypanosomiasis in humans: a new potential pandemic route for chagas parasites. | the trypanosoma cruzi infection endemic in latin america has now spread to several countries across four continents; this endemic involves triatomine vector-free protists. we hypothesised that the sexual transmission of t. cruzi contributes to the ongoing spread of chagas disease. | 2017 | 28591404 |
| insights from tissue-specific transcriptome sequencing analysis of triatoma infestans. | triatoma infestans is an insect of subfamily triatominae (hemiptera: reduviidae) and an important vector of trypanosoma cruzi, the etiologic agent of human chagas disease. in this work we reported a transcriptome assembly and annotation of t. infestans heads obtained by next generation sequencing (ngs) technologies. | 2017 | 28591407 |
| trans-sialidase overcomes many antigens to be used as a vaccine candidate against trypanosoma cruzi. | the development of vaccines against trypanosoma cruzi remains in an exploratory stage. despite several antigen candidates have been evaluated, a comparison among the performance of the immunogens cannot be carried out because the available reports differ in formulations and infection model. in this work, we compared the protective capacity of seven t. cruzi antigens in the same model of five new antigens and two well-established candidates. materials & methods: we evaluated highly immunogenic pr ... | 2017 | 28595515 |
| omega-3 supplementation on inflammatory markers in patients with chronic chagas cardiomyopathy: a randomized clinical study. | several studies have been focusing on the effect of omega-3 polyunsaturated fatty acids on modulation of inflammatory markers in several cardiopathies. although immunoregulatory dysfunction has been associated to the chronic cardiac involvement in chagas disease, there is no study examining the effects of omega-3 supplementation in these patients. we investigated the effects of omega-3 pufas on markers of inflammation and lipid profile in chronic chagas cardiomyopathy patients. | 2017 | 28599665 |
| an elisa immunoassay employing a conserved leishmania hypothetical protein for the serodiagnosis of visceral and tegumentary leishmaniasis in dogs and humans. | in the present study, a conserved leishmania hypothetical protein, namely lihypa, was evaluated for the serodiagnosis of visceral and tegumentary leishmaniasis in dogs and humans. this protein showed a high amino acid sequence homology between viscerotropic and cutaneotropic leishmania species. an enzyme-linked immunosorbent assay (elisa) was developed using the recombinant antigen (rlihypa), in addition to the a2 protein and two parasite antigenic preparations, which were used as controls. rega ... | 2017 | 28602279 |
| qpcr for the detection of foodborne trypanosoma cruzi. | here we presented a potential real-time pcr (qpcr) method with public health importance and relevance for detection of trypanosoma cruzi in açai pulp. there is not a current process to identify t. cruzi in açai, that ensures innocuity of this food concerning oral transmission. first, six new primers were designed using the dna sequences of t. cruzi y152 and emerald strains obtained from genbank. for primers evaluation and titration they were validated regarding the amplification and not with the ... | 2017 | 28602861 |
| jaburetox-induced toxic effects on the hemocytes of rhodnius prolixus (hemiptera: reduviidae). | jaburetox is a recombinant peptide derived from a canavalia ensiformis urease that presents toxic effects upon several species of insects, phytopathogenic fungi and yeasts of medical importance. so far, no toxicity of jaburetox to mammals has been shown. previous reports have identified biochemical targets of this toxic peptide in insect models, although its mechanism of action is not completely understood. in this work, we aimed to characterize the effects of jaburetox in hemolymphatic insect c ... | 2017 | 28602911 |
| seasonality and temperature-dependent flight dispersal of triatoma infestans (hemiptera: reduviidae) and other vectors of chagas disease in western argentina. | flight dispersal of triatominae is affected by climatic conditions and determines the spatiotemporal patterns of house invasion and transmission of trypanosoma cruzi chagas (kinetoplastida: trypanosomatidae). we investigated the detailed time structure and temperature dependencies of flight occurrence of triatoma infestans klug (hemiptera: reduviidae) and other triatomine species in a rural village of western argentina by taking advantage of the attraction of adult triatomines to artificial ligh ... | 2017 | 28605522 |
| triatokey: a web and mobile tool for biodiversity identification of brazilian triatomine species. | triatomines are blood-sucking insects that transmit the causative agent of chagas disease, trypanosoma cruzi. despite being recognized as a difficult task, the correct taxonomic identification of triatomine species is crucial for vector control in latin america, where the disease is endemic. in this context, we have developed a web and mobile tool based on postgresql database to help healthcare technicians to overcome the difficulties to identify triatomine vectors when the technical expertise i ... | 2017 | 28605769 |
| the mechanisms of ventricular arrhythmia in chagas disease. | 2017 | 28606682 | |
| proteomic analysis of the kissing bug rhodnius prolixus antenna. | reception of odorants is essential in insects' life since the chemical signals in the environment (=semiochemicals) convey information about availability of hosts for a blood meal, mates for reproduction, sites for oviposition and other relevant information for fitness in the environment. once they reach the antennae, these semiochemicals bind to odorant-binding proteins and are transported through the sensillar lymph until reach the odorant receptors. such perireceptor events, particularly the ... | 2017 | 28606853 |
| inhibition of host cell lysosome spreading by trypanosoma cruzi metacyclic stage-specific surface molecule gp90 downregulates parasite invasion. | successful infection by trypanosoma cruzi, the agent of chagas' disease, is critically dependent on host cell invasion by metacyclic trypomastigote (mt) forms. two main metacyclic stage-specific surface molecules, gp82 and gp90, play determinant roles in target cell invasion in vitro and in oral t. cruzi infection in mice. the structure and properties of gp82, which is highly conserved among t. cruzi strains, are well known. information on gp90 is still rather sparse. here, we attempted to fill ... | 2017 | 28607099 |
| functional characterization of aquaporin-like genes in the human bed bug cimex lectularius. | the bed bug cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit trypanosoma cruzi under experimental laboratory conditions. aquaporins (aqps) are water channel proteins that are essential in biological organisms. c. lectularius are constantly exposed to water-related stress, suggesting that aqps may offer novel control avenues. we identified and cloned four aqps from c. lectularius, assessed tissue and lifestage-specific expression, and charact ... | 2017 | 28607409 |
| use of zymography in trypanosomiasis studies. | zymography assay is a semiquantitative technique, very sensitive, and commonly used to determine metalloproteinase levels in different types of biological samples, including tissues, cells, and extracts of protein. samples containing metalloproteinases are loaded onto a polyacrylamide gel containing sodium dodecyl sulphate (sds) and a specific substrate (gelatin, casein, collagen, etc.). then proteins are allowed to migrate under an electric current and the distance of migration is inversely cor ... | 2017 | 28608214 |
| [estimating demand for anti-chagas drugs: a contribution for access in latin america]. | describe a tool to estimate demand for benznidazole and nifurtimox to treat chagas disease, and report on its implementation in a group of latin american countries. | 2017 | 28614468 |
| the non-canonical substrates of trypanosoma cruzi tyrosine and aspartate aminotransferases: branched-chain amino acids. | trypanosoma cruzi, the etiological agent of chagas disease, lacks genes that encode canonical branched-chain aminotransferases. however, early studies showed that when epimastigotes were grown in the presence of (14) c1 -dl-leucine, the label was incorporated into various intermediates. more recently, our studies provided evidence that t. cruzi epimastigotes display a single atp-dependent and saturable transport system that enables epimastigotes to uptake branched-chain amino acids (bcaas) from ... | 2017 | 28618210 |
| canine macrophage dh82 cell line as a model to study susceptibility to trypanosoma cruzi infection. | trypanosoma cruzi is an obligatory intracellular protozoan parasite, and it is the etiological agent of chagas' disease that is endemic in the americas. in addition to humans, a wide spectrum of mammals can be infected by t. cruzi, including dogs. dogs develop acute and chronic disease, similar to human infection. t. cruzi can infect almost all cell types and after cell invasion, the metacyclics trypomastigotes localize in the cytoplasm, where they transform into amastigotes, the replicative for ... | 2017 | 28620374 |
| effects of (1e,4e)-2-methyl-1,5-bis(4-nitrophenyl)penta-1,4-dien-3-one on trypanosoma cruzi and its combinational effect with benznidazole, ketoconazole, or fluconazole. | this study reports the activity induced by (1e,4e)-2-methyl-1,5-bis(4-nitrophenyl)penta-1,4-dien-3-one (a3k2a3) against trypanosoma cruzi. this compound showed trypanocidal activity against the multiplicative epimastigote and amastigote forms of this protozoan, with ic50 values of 1.99 ± 0.17 and 1.20 ± 0.16 μm, respectively, and ec50 value of 15.57 ± 0.34 μm against trypomastigotes. the combination of a3k2a3 with benznidazole or ketoconazole demonstrated strong synergism, increasing effectivene ... | 2017 | 28620619 |
| repositioning fda drugs as potential cruzain inhibitors from trypanosoma cruzi: virtual screening, in vitro and in vivo studies. | chagas disease (cd) is a neglected disease caused by the parasite trypanosoma cruzi, which affects underdeveloped countries. the current drugs of choice are nifurtimox and benznidazole, but both have severe adverse effects and less effectivity in chronic infections; therefore, the need to discover new drugs is essential. a computer-guided drug repositioning method was applied to identify potential fda drugs (approved and withdrawn) as cruzain (cz) inhibitors and trypanocidal effects were confirm ... | 2017 | 28629155 |
| [what do the numbers tell us about the temporal evolution of chagas' disease?] | chagas disease remains highly prevalent in chile, especially between the regions of arica and parinacota, and coquimbo. since 1999 it is considered that in chile the vector transmission was interrupted. under this premise, the epidemiological dynamics should be changing. we analyzed the evolution of the prevalence of chagas' disease analyzing 64,995 xenodiagnosis performed in the laboratory of parasitology of the faculty of medicine of the university of chile between 1949 and 2014. the evolution ... | 2017 | 28632825 |
| developing a casper survey to assess the prevalence of risk factors for neglected tropical diseases in texas. | while more than a billion people live at risk of neglected tropical diseases in areas of asia, sub-saharan africa, and latin america, the degree to which such diseases burden countries like the united states is currently unclear. even though many neglected tropical diseases such as dengue, leishmaniasis, and chagas disease are not endemic to the united states, the possibility of their emergence is noteworthy, especially in states like texas, which has high levels of poverty, a large immigrant po ... | 2017 | 28636445 |
| bioinformatics insights on targets receptors of amiodarone in human and acanthamoeba castellanii. | amiodarone is prescribed for certain cardiac arrhythmias in medical practice. the drug targets and inhibits voltage dependent sodium (na+v), calcium (ca+2v), potassium (k+v) channels, and enzymes like cytochrome p450 and oxidosqualene cyclase. past studies have shown that amiodarone exerts anti-amoebic effects against trypanosoma cruzi and acanthamoeba castellanii. | 2017 | 28637420 |
| [on site-screening for chagas disease supported by a community intervention: can it improve accessibility for diagnosis and treatment?] | 2017 | 28641864 | |
| benznidazole treatment safety: the médecins sans frontières experience in a large cohort of bolivian patients with chagas' disease. | up to half of patients with chagas' disease under benznidazole treatment present adverse drug reactions (adrs) and up to one-third do not complete standard treatment. | 2017 | 28645201 |
| subcellular localization of glycolytic enzymes and characterization of intermediary metabolism of trypanosoma rangeli. | trypanosoma rangeli is a hemoflagellate protist that infects wild and domestic mammals as well as humans in central and south america. although this parasite is not pathogenic for human, it is being studied because it shares with trypanosoma cruzi, the etiological agent of chagas' disease, biological characteristics, geographic distribution, vectors and vertebrate hosts. several metabolic studies have been performed with t. cruzi epimastigotes, however little is known about the metabolism of t. ... | 2017 | 28645481 |
| synthesis, in vitro and in vivo giardicidal activity of nitrothiazole-nsaid chimeras displaying broad antiprotozoal spectrum. | we designed and synthesized five new 5-nitrothiazole-nsaid chimeras as analogues of nitazoxanide, using a dcc-activated amidation. compounds 1-5 were tested in vitro against a panel of five protozoa: 2 amitochondriates (giardia intestinalis, trichomonas vaginalis) and 3 kinetoplastids (leishmania mexicana, leishmania amazonensis and trypanosoma cruzi). all chimeras showed broad spectrum and potent antiprotozoal activities, with ic50 values ranging from the low micromolar to nanomolar order. comp ... | 2017 | 28645659 |
| increased of the hepatocytes and splenocytes apoptosis accompanies clinical improvement and higher survival in mice infected with trypanosoma cruzi and treated with highly diluted lycopodium clavatum. | recent evidence includes apoptosis as a defense against trypanosoma cruzi infection, which promotes an immune response in the host induced by t cells, type 1, 2 and 17. currently, there is no medicine completely preventing the progression of this disease. we investigated the immunological and apoptotic effects, morbidity and survival of mice infected with t. cruzi and treated with dynamized homeopathic compounds 13c: kalium causticum (gcaus), conium maculatum, (gcon), lycopodium clavatum (gly) a ... | 2017 | 28645772 |
| synthesis and biological evaluation of 2-methyl-1h-benzimidazole-5-carbohydrazides derivatives as modifiers of redox homeostasis of trypanosoma cruzi. | twelve novel benzimidazole derivatives were synthesized and their in vitro activities against epimastigotes of trypanosoma cruzi were evaluated. two derivatives (6 and 7), which have 4-hydroxy-3-methoxyphenyl moiety in their structures, proved to be the most active in inhibiting the parasite growth. compound 6 showed a trypanocidal activity higher than benznidazole (ic50=5µm and 7.5µm, respectively) and less than nifurtimox (ic50=3.6µm). in addition, the ability of 6 and 7 to modify the redox ho ... | 2017 | 28648464 |
| catalase expression impairs oxidative stress-mediated signalling in trypanosoma cruzi. | trypanosoma cruzi is exposed to oxidative stresses during its life cycle, and amongst the strategies employed by this parasite to deal with these situations sits a peculiar trypanothione-dependent antioxidant system. remarkably, t. cruzi's antioxidant repertoire does not include catalase. in an attempt to shed light on what are the reasons by which this parasite lacks this enzyme, a t. cruzi cell line stably expressing catalase showed an increased resistance to hydrogen peroxide (h2o2) when comp ... | 2017 | 28653592 |
| a brief view of the surface membrane proteins from trypanosoma cruzi. | trypanosoma cruzi is the causal agent of chagas' disease which affects millions of people around the world mostly in central and south america. t. cruzi expresses a wide variety of proteins on its surface membrane which has an important role in the biology of these parasites. surface molecules of the parasites are the result of the environment to which the parasites are exposed during their life cycle. hence, t. cruzi displays several modifications when they move from one host to another. due to ... | 2017 | 28656101 |
| identification of potential trypanothione reductase inhibitors among commercially available [formula: see text]-carboline derivatives using chemical space, lead-like and drug-like filters, pharmacophore models and molecular docking. | american trypanosomiasis or chagas disease caused by the protozoan trypanosoma cruzi (t. cruzi) is an important endemic trypanosomiasis in central and south america. this disease was considered to be a priority in the global plan to combat neglected tropical diseases, 2008-2015, which indicates that there is an urgent need to develop more effective drugs. the development of new chemotherapeutic agents against chagas disease can be related to an important biochemical feature of t. cruzi: its redo ... | 2017 | 28656524 |
| development and assessment of a new cage-like particle adjuvant. | to obtain and assess stable cage-like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. | 2017 | 28664569 |
| death of adrenocortical cells during murine acute t. cruzi infection is not associated with tnf-r1 signaling but mostly with the type ii pathway of fas-mediated apoptosis. | earlier studies from our laboratory demonstrated that acute experimental trypanosoma cruzi infection promotes an intense inflammation along with a sepsis-like dysregulated adrenal response characterized by normal levels of acth with raised glucocorticoid secretion. inflammation was also known to result in adrenal cell apoptosis, which in turn may influence hpa axis uncoupling. to explore factors and pathways which may be involved in the apoptosis of adrenal cells, together with its impact on the ... | 2017 | 28666938 |
| targeting the active sites of malarial proteases for antimalarial drug discovery: approaches, progress and challenges. | malaria is an infectious disease causing vast mortality and morbidity worldwide. although antimalarial drugs are effective in several parts of the world, there is a serious threat to malaria control as malaria parasites are continuously developing widespread resistance against currently available antimalarial drugs, including artemisinin. such widespread antimalarial drug resistance confirms the need to improve the efficacy of existing or new drugs as well as to develop alternative treatments th ... | 2017 | 28668681 |
| chagas disease. | chagas disease is an anthropozoonosis from the american continent that has spread from its original boundaries through migration. it is caused by the protozoan trypanosoma cruzi, which was identified in the first decade of the 20th century. once acute infection resolves, patients can develop chronic disease, which in up to 30-40% of cases is characterised by cardiomyopathy, arrhythmias, megaviscera, and, more rarely, polyneuropathy and stroke. even after more than a century, many challenges rema ... | 2017 | 28673423 |
| genetic adjuvantation of a cell-based therapeutic vaccine for amelioration of chagasic cardiomyopathy. | chagas disease, caused by infection with the protozoan parasite trypanosoma cruzi, is a leading cause of heart disease ("chagasic cardiomyopathy") in latin america, disproportionately affecting people in resource-poor areas. the efficacy of currently approved pharmaceutical treatments is limited mainly to acute infection, and there are no effective treatments for the chronic phase of the disease. preclinical models of chagas disease have demonstrated that antigen-specific cd8(+) gamma interferon ... | 2017 | 28674032 |
| screening the medicines for malaria venture pathogen box across multiple pathogens reclassifies starting points for open-source drug discovery. | open-access drug discovery provides a substantial resource for diseases primarily affecting the poor and disadvantaged. the open-access pathogen box collection is comprised of compounds with demonstrated biological activity against specific pathogenic organisms. the supply of this resource by the medicines for malaria venture has the potential to provide new chemical starting points for a number of tropical and neglected diseases, through repurposing of these compounds for use in drug discovery ... | 2017 | 28674055 |
| early clinics of the cardiac forms of chagas' disease: discovery and study of original medical files (1909-1915). | we have uncovered 80 medical files corresponding to original cases of chagas' disease used for the classical description of the acute and cardiac forms of the disease. sixty of them were diagnosed cardiac forms of the disease. the detailed clinical description of these 60 files is in excellent agreement with the nosography of progressive heart disease given by chagas in his original 1922 paper. the reports we had access to, characterize a novel form of cardiac disease, dominated by progressive a ... | 2017 | 28676242 |
| pharmacodynamics and follow-up period in the treatment of human trypanosoma cruzi infections with posaconazole. | 2017 | 28683978 | |
| rapid detection of trypanosoma cruzi by colorimetric loop-mediated isothermal amplification (lamp): a potential novel tool for the detection of congenital chagas infection. | early diagnosis of congenital trypanosomacruzi transmission in newborns is essential because babies show high indices of cure. conventional diagnosis is based on microscopic examination and serology. molecular diagnosis is a promising alternative to replace conventional diagnosis, although it is not well suited for adoption in laboratories with limited resources. isothermal dna amplification methods have the advantage of not requiring expensive equipment. the aim of this work was to apply loop-m ... | 2017 | 28684052 |
| complete mitochondrial genome of triatoma infestans (hemiptera, reduviidae, triatominae), main vector of chagas disease. | the complete mitogenome of triatoma infestans, the main vector of chagas disease in south america, was obtained by combining illumina and sanger sequencing sequence data. the 17,301bp long genome contains 13 protein-coding genes, 22 transfer rnas, 2 ribosomal rnas, and a control region. the number, order and orientation of mitochondrial genes are the same as in t. dimidiata, the only triatominae mt genome published so far. the main differences between both mitogenomes are found in the control re ... | 2017 | 28684375 |
| in vitro and in vivo assessment of newer quinoxaline-oxadiazole hybrids as antimicrobial and antiprotozoal agents. | a new series of n-(substituted-phenyl)-2-[5-(quinoxalin-2-yloxymethyl)-[1,3,4] oxadiazol-2-ylsulfanyl]-acetamides (5a-o) was designed and synthesised from the parent compound 2-hydroxy quinoxaline (1) through a multistep reaction sequence and was characterised by spectral and elemental analyses. all of the compounds synthesised were evaluated for their antimicrobial and antiprotozoal activities. the results revealed that quinoxaline-based 1,3,4-oxadiazoles displayed promising antibacterial, anti ... | 2017 | 28687457 |
| a survey of zoonotic pathogens carried by house mouse and black rat populations in yucatan, mexico. | the house mouse (mus musculus) and the black rat (rattus rattus) are reservoir hosts for zoonotic pathogens, several of which cause neglected tropical diseases (ntds). studies of the prevalence of these ntd-causing zoonotic pathogens, in house mice and black rats from tropical residential areas are scarce. three hundred and two house mice and 161 black rats were trapped in 2013 from two urban neighbourhoods and a rural village in yucatan, mexico, and subsequently tested for trypanosoma cruzi, hy ... | 2017 | 28689507 |
| extended screening of trypanosoma cruzi among the offspring of infected women. barcelona north metropolitan area, catalonia (spain), 2005-2016. | to date, very little data is available on the extensive, familiar, serological screening of trypanosoma cruzi from infected-index cases. as it is a parasite with possibility of mother-to-child fetal transmission, the study of the offspring of chronically infected women has a special relevance. | 2017 | 28689672 |
| salivary gland transcripts of the kissing bug, panstrongylus chinai, a vector of chagas disease. | the saliva of hematophagous arthropods injected during blood feeding contains potent pharmacologically active components to counteract the host hemostatic and inflammatory systems. in the present study, dominant salivary gland transcripts of panstrongylus chinai, a vector of chagas disease, were analyzed by sequencing randomly selected clones of the salivary gland cdna library. this analysis showed that 56.5% of the isolated transcripts coded for putative secreted proteins, of which 73.7% coded ... | 2017 | 28690145 |
| betulinic acid induces cell death by necrosis in trypanosoma cruzi. | chagas' disease is a neglected disease caused by the protozoan parasite trypanosoma cruzi and constitutes a serious health problem worldwide. the treatment is limited, with variable efficacy of benznidazole and nifurtimox. betulinic acid (ba), a triterpene, can be found in medicinal herbs and has a wide variety of biological and pharmacological activities. the objective was to evaluate betulinic acid effects on the cell death mechanism in trypanosoma cruzi strain y. ba inhibited the growth of ep ... | 2017 | 28690148 |
| the involvement of rhopr-crf/dh in feeding and reproduction in the blood-gorging insect rhodnius prolixus. | rhodnius prolixus is a blood-gorging insect and a vector for human chagas disease. the insect transmits the disease following feeding, when it excretes urine and feces contaminated with the trypanosoma cruzi parasite. a corticotropin-releasing factor-like peptide acts as a diuretic hormone in r. prolixus (rhopr-crf/dh); however, its distribution throughout the insect's central nervous system (cns) and the expression of its receptor in feeding-related tissue as well as the female reproductive sys ... | 2017 | 28694056 |
| avian antibodies (igy) against trypanosoma cruzi: purification and characterization studies. | trypanosoma cruzi is a flagellated protozoan belonging to the trypanosomatidae family, the etiologic agent of chagas disease. currently, there is neither a licensed vaccine nor effective treatment, characterizing an unmet clinical need. the igy refers to the egg yolk immunoglobulin (y=yolk) and its production and use are subjects of many studies due to the diversity of its diagnostic and therapeutic applications. several researchers have shown that the use of specific igy may prevent and/or cont ... | 2017 | 28697990 |
| pacemaker implants in children and adolescents with chagas disease in brazil: 18-year incidence. | chagas disease continues to be a serious public health problem, and accounts for 25-30% of the indications for cardiac stimulation in brazil. | 2017 | 28699977 |
| facing chagas disease. | 2017 | 28700043 | |
| chagas disease: review of needs, neglect, and obstacles to treatment access in latin america. | after more than one century since its discovery, chagas disease is still extremely prevalent in 21 latin american countries. chagas disease is one of the most concerning public health problems in latin america; the overall cost of cd treatment is approximately 7 billion united states dollars per year and it has a strong social impact on populations. little progress has been made regarding the access to diagnosis and treatment at the primary health care level, calling into question the current po ... | 2017 | 28700045 |
| pharmacotherapeutic follow-up of patients with chagas disease using benznidazole: drug-related problems and pharmaceutical interventions. | benznidazole (bnz) is a drug available for the etiological treatment of chagas disease. however, this drug is toxic and has a limited effectiveness on the chronic phase of this disease, often leading to poor treatment adherence. | 2017 | 28700051 |
| influence of environmental enrichment on the behavior and physiology of mice infected by trypanosoma cruzi. | enriched environments normally increase behavioral repertoires and diminish the expression of abnormal behaviors and stress-related physiological problems in animals. although it has been shown that experimental animals infected with microorganisms can modify their behaviors and physiology, few studies have evaluated how environmental enrichment affects these parameters. this study aimed to evaluate the effects of environmental enrichment on the behavior and physiology of confined mice infected ... | 2017 | 28700052 |
| taxonomic status of panstrongylus herreri wygodzinsky, 1948 and the number of chagas disease vectors. | 2017 | 28700072 | |
| modelling the influence of host community composition in a sylvatic trypanosoma cruzi system. | species composition of wild reservoir hosts can influence the transmission and maintenance of multi-host vector borne pathogens. the 'pace of life' hypothesis proposes that the life history strategy of reservoir hosts can influence pathogen transmission of vector borne generalist pathogens. we use empirical data to parameterize a mathematical model that investigates the impacts of host life history traits on vector transmission dynamics of the vector-borne multi-host parasite trypanosoma cruzi i ... | 2017 | 28701240 |
| prevalence of trypanosoma cruzi infection in dogs and small mammals in nuevo león, mexico. | chagas disease, caused by the protozoan trypanosoma cruzi, is an important public health concern in areas extending from south america northward into the southern united states of america. although this hemoflagellate has many wild and domestic mammalians reported as reservoir hosts, studies on this subject are scarce in nuevo león state, a region located in northeastern mexico. this cross-sectional study showed that the general prevalence of t. cruzi infection in nuevo león state was 14.5% (35/ ... | 2017 | 28705488 |
| an infrequent cause of apical ventricular aneurysm in the united states. | chronic chagas cardiomyopathy (ccc) is a late complication of chagas disease with various manifestations including arrhythmia, heart failure, thromboembolism, and stroke. in a patient with symptoms of heart failure and left ventricular apical aneurysm unexplained by structural heart or coronary vascular abnormalities, ccc should be strongly considered and inquiry made about exposure status. typical electrocardiographic findings of bundle branch block, complete heart blocks, and ventricular arrhy ... | 2017 | 28706597 |
| inhibitors of glycosomal protein import provide new leads against trypanosomiasis. | vector-borne trypanosomatid parasite infections in tropical and sub-tropical countries constitute a major threat to humans and livestock. trypanosoma brucei parasites are transmitted by tsetse fly and lead to african sleeping sickness in humans and nagana in cattle. in latin american countries, trypanosoma cruzi infections spread by triatomine kissing bugs lead to chagas disease. various species of leishmania transmitted to humans by phlebotomine sandflies manifest in a spectrum of diseases term ... | 2017 | 28706938 |
| correction for ekanayake et al., "epigenetic regulation of transcription and virulence in trypanosoma cruzi by o-linked thymine glucosylation of dna". | 2017 | 28710121 | |
| health care importance of treponema pallidum, chagas' disease and human immunodeficiency virus 1 among amerindians of argentina: an observational study. | the objective of this study was to estimate the prevalence of treponema pallidum, trypanosoma cruzi and human immunodeficiency virus 1 (hiv-1) in five amerindian populations of argentina. a retrospective study was conducted among 857 amerindian populations (112 kollas, 298 mbyá-guaraníes, 79 sagua huarpes, 368 wichis) from 2007 to 2010. screening and confirmation of t. pallidum, t. cruzi and hiv-1 were performed. t. pallidum and t. cruzi infections were detected in all communities with an overal ... | 2017 | 28712508 |
| cholinesterase inhibition reduces arrhythmias in asymptomatic chagas disease. | parasympathetic dysfunction may play a role in the genesis of arrhythmias in chagas disease. | 2017 | 28715142 |
| homology modeling of leishmania donovani enolase and its molecular interaction with novel inhibitors. | the treatment of indian tropical disease such as kala-azar is likely to be troublesome to the clinicians as ampb- and miltefosine-resistant leishmania donovani has been reported. the rationale behind designed a novel inhibitors of model of l. donovani enolase and performing a binding study with its inhibitors to gain details of the interaction between protein residues and ligand molecules. | 2017 | 28717332 |
| high triatoma brasiliensis densities and trypanosoma cruzi prevalence in domestic and peridomestic habitats in the state of rio grande do norte, brazil: the source for chagas disease outbreaks? | abstracta total of 2,431 triatoma brasiliensis were collected from 39 populations of paraíba (pb) and rio grande do norte (rn) states, brazil. in pb, trypanosoma cruzi infection was not detected in either peridomestic or domestic vector populations. in contrast, in rn, t. brasiliensis was detected with high parasite prevalence in these ecotopes (30.7-40.0%). moreover, peridomicile insect population densities were more than double the average densities of all other settings evaluated (19.17 versu ... | 2017 | 28719275 |
| simplified membrane feeding of bloodsucking triatomines (hemiptera: reduviidae). | laboratory-reared hematophagous triatomines that are the main vectors of chagas disease are generally fed on small vertebrates or on blood-filled membrane devices. such devices allow a large-scale rearing of these vectors without sacrificing host animals but are almost always expensive, fragile, not easily purchased, and usually difficult to use because they need to be sterilized before and carefully cleaned after each feeding. we present here a simple device to feed triatomines that is composed ... | 2017 | 28720489 |
| the immunomodulatory effects of the enalapril in combination with benznidazole during acute and chronic phases of the experimental infection with trypanosoma cruzi. | trypanosoma cruzi infection triggers a chronic inflammatory process responsible for the alterations in the extracellular matrix and functionality of the heart. the angiotensin converting enzyme (ace) inhibitors affects t. cruzi in vitro surveillance and modulates in vivo some inflammatory mediators. in this study, we investigated the treatment with an ace inhibitor (enalapril) and the benznidazole (bz) in a single and combination therapies (ct) in c57bl/6 mice infected with vl-10 strain of the t ... | 2017 | 28720491 |
| prevention of congenital chagas disease by benznidazole treatment in reproductive-age women. an observational study. | since the decline in new cases of infection by insect/vector, congenital chagas disease has become more relevant in the transmission of chagas disease. treatment with benznidazole significantly reduces the parasitemia, which constitutes an important factor linked to vertical transmission. the objective of this study was to evaluate whether treatment with benznidazole previously administered to women of childbearing age can prevent or reduce the incidence of new cases of congenital chagas disease ... | 2017 | 28720492 |
| study of the salivary glands in triatominae (hemiptera, reduviidae, triatominae): their color and application to the chagas disease vector evolution. | chagas disease is caused by trypanosoma cruzi and transmitted by feces of a triatomine that has the habit of defecating during blood feeding. the salivary glands of triatomines are important to hematophagy because their saliva is rich in anticoagulant and hemolytic proteins. the salivary glands of some rhodnius species analyzed are reddish due to the presence of nitrophorins (antihemostatic activity). the present study aimed to analyze the color pattern of the salivary glands of 67 triatomine sp ... | 2017 | 28722578 |
| silencing of two vitellogenin genes inhibits oviposition in the chagas disease vector triatoma infestans (hemiptera: reduviidae). | rna interference caused by exogenous double-stranded rna (dsrna) is used to downregulate crucial genes to control insects. the reproductive success of all oviparous species depends on vitellogenin (vg) biosynthesis and its accumulation in the developing oocytes. adult females of triatoma infestans were independently injected with two vg dsrnas (vg1 dsrna or vg2 dsrna) or nuclease-free water (control) 24 hours before feeding, and a group of adult females not injected was also analyzed (control). ... | 2017 | 28722589 |
| 2b-rad genotyping for population genomic studies of chagas disease vectors: rhodnius ecuadoriensis in ecuador. | rhodnius ecuadoriensis is the main triatomine vector of chagas disease, american trypanosomiasis, in southern ecuador and northern peru. genomic approaches and next generation sequencing technologies have become powerful tools for investigating population diversity and structure which is a key consideration for vector control. here we assess the effectiveness of three different 2b restriction site-associated dna (2b-rad) genotyping strategies in r. ecuadoriensis to provide sufficient genomic res ... | 2017 | 28723901 |
| evolution of anti-trypanosoma cruzi antibody production in patients with chronic chagas disease: correlation between antibody titers and development of cardiac disease severity. | chagas disease is one of the most important endemic infections in latin america affecting around 6-7 million people. about 30-50% of patients develop the cardiac form of the disease, which can lead to severe cardiac dysfunction and death. in this scenario, the identification of immunological markers of disease progression would be a valuable tool for early treatment and reduction of death rates. in this observational study, the production of anti-trypanosoma cruzi antibodies through a retrospect ... | 2017 | 28723905 |
| comparative repeatome analysis on triatoma infestans andean and non-andean lineages, main vector of chagas disease. | triatoma infestans is the most important chagas disease vector in south america. two main evolutionary lineages, named andean and non-andean, have been recognized by geographical distribution, phenetic and genetic characteristics. one of the main differences is the genomic size, varying over 30% in their haploid dna content. here we realize a genome wide analysis to compare the repetitive genome fraction (repeatome) between both lineages in order to identify the main repetitive dna changes occur ... | 2017 | 28723933 |
| systemic insecticide treatment of the canine reservoir of trypanosoma cruzi induces high levels of lethality in triatoma infestans, a principal vector of chagas disease. | despite large-scale reductions in chagas disease prevalence across central and south america, trypanosoma cruzi infection remains a considerable public health problem in the gran chaco region where vector-borne transmission persists. in these communities, peridomestic animals are major blood-meal sources for triatomines, and household presence of infected dogs increases t. cruzi transmission risk for humans. to address the pressing need for field-friendly, complementary methods to reduce triatom ... | 2017 | 28724448 |
| performance assessment of a trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays. | diagnosing chronic chagas disease (cd) requires antibody--antigen detection methods, traditionally based on enzymatic assay techniques whose performance depend on the type and quality of antigen used. previously, 4 recombinant chimeric proteins from instituto de biologia molecular do paraná (ibmp-8.1 to -8.4) comprising immuno-dominant regions of diverse trypanosoma cruzi antigens showed excellent diagnostic performance in enzyme-linked immunosorbent assays. considering that next-generation plat ... | 2017 | 28724556 |
| study of infectious diseases in archaeological bone material - a dataset. | bones of human and ground sloth remains were analyzed for presence of trypanosoma cruzi by conventional pcr using primers tc, tc1 and tc2. sequence results amplified a fragment with the same product size as the primers (300 and 350pb). amplified pcr product was sequenced and analyzed on genbank, using blast. although these sequences did not match with these parasites they showed high amplification with species of bacteria. this article presents the methodology used and the alignment of the seque ... | 2017 | 28725674 |
| analytical sensitivity and specificity of a loop-mediated isothermal amplification (lamp) kit prototype for detection of trypanosoma cruzi dna in human blood samples. | this study aimed to assess analytical parameters of a prototype lamp kit that was designed for detection of trypanosoma cruzi dna in human blood. the prototype is based on the amplification of the highly repetitive satellite sequence of t.cruzi in microtubes containing dried reagents on the inside of the caps. the reaction is carried out at 65°c during 40 minutes. calcein allows direct detection of amplified products with the naked eye. inclusivity and selectivity were tested in purified dna fro ... | 2017 | 28727723 |
| small subunit ribosomal metabarcoding reveals extraordinary trypanosomatid diversity in brazilian bats. | bats are a highly successful, globally dispersed order of mammals that occupy a wide array of ecological niches. they are also intensely parasitized and implicated in multiple viral, bacterial and parasitic zoonoses. trypanosomes are thought to be especially abundant and diverse in bats. in this study, we used 18s ribosomal rna metabarcoding to probe bat trypanosome diversity in unprecedented detail. | 2017 | 28727769 |
| comparative study and analytical verification of pcr methods for the diagnosis of congenital chagas disease. | congenital infection is currently the first cause of new cases of chagas disease in argentina and nonendemic areas worldwide. its diagnosis is of utmost importance to guarantee curative treatment. to improve such diagnosis, a transfer process of pcr tests to the national laboratory network has been initiated. we performed a comparative study of four pcr assays [two end-point pcr and two duplex real-time quantitative pcr (qpcr) procedures] to detect trypanosoma cruzi dna in blood samples. because ... | 2017 | 28727985 |