Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| structure, kinetic characterization and subcellular localization of the two ribulose 5-phosphate epimerase isoenzymes from trypanosoma cruzi. | the enzyme of the pentose phosphate pathway (ppp) ribulose-5-phosphate-epimerase (rpe) is encoded by two genes present in the genome of trypanosoma cruzi cl brener clone: tcrpe1 and tcrpe2. despite high sequence similarity at the amino acid residue level, the recombinant isoenzymes show a strikingly different kinetics. whereas tcrpe2 follows a typical michaelian behavior, tcrpe1 shows a complex kinetic pattern, displaying a biphasic curve, suggesting the coexistence of -at least- two kinetically ... | 2017 | 28207833 |
| polymorphisms of blood forms and in vitro metacyclogenesis of trypanosoma cruzi i, ii, and iv. | trypanosoma cruzi is the etiologic agent of american trypanosomiasis has broad biological and genetic diversity. remaining to be studied are polymorphisms of the blood forms and metacyclogenesis of different t. cruzi discrete typing units (dtus). our goal was to evaluate the relationship between t. cruzi dtus, the morphology of blood trypomastigotes, and in vitro metacyclogenesis. t. cruzi strains that pertained to dtus tci, tcii, and tciv from different brazilian states were used. parameters th ... | 2017 | 28212811 |
| dissecting biochemical peculiarities of the atpase activity of tcsub2, a component of the mrna export pathway in trypanosoma cruzi. | the rna helicase dead-box protein sub2 (yeast)/uap56 (mammals) is conserved across eukaryotes and is essential for mrna export in trypanosomes. despite the high conservation of sub2 in lower eukaryotes such as trypanosoma cruzi, the low conservation of other mrna export factors raises questions regarding whether the mode of action of tcsub2 is similar to that of orthologs from other eukaryotes. mutation of the conserved k87 residue of tcsub2 abolishes atpase activity, showing that its atpase dom ... | 2017 | 28212935 |
| evidence of the presence of a calmodulin-sensitive plasma membrane ca(2+)-atpase in trypanosoma equiperdum. | trypanosoma equiperdum belongs to the subgenus trypanozoon, which has a significant socio-economic impact by limiting animal protein productivity worldwide. proteins involved in the intracellular ca(2+) regulation are prospective chemotherapeutic targets since several drugs used in experimental treatment against trypanosomatids exert their action through the disruption of the parasite intracellular ca(2+) homeostasis. therefore, the plasma membrane ca(2+)-atpase (pmca) is considered as a potenti ... | 2017 | 28213174 |
| synthesis and biological evaluation of potential inhibitors of the cysteine proteases cruzain and rhodesain designed by molecular simplification. | analogues of 8-chloro-n-(3-morpholinopropyl)-5h-pyrimido[5,4-b]indol-4-amine 1, a known cruzain inhibitor, were synthesized using a molecular simplification strategy. five series of analogues were obtained: indole, pyrimidine, quinoline, aniline and pyrrole derivatives. the activity of the compounds was evaluated against the enzymes cruzain and rhodesain as well as against trypanosoma cruzi amastigote and trypomastigote forms. the 4-aminoquinoline derivatives showed promising activity against bo ... | 2017 | 28215783 |
| likely autochthonous transmission of trypanosoma cruzi to humans, south central texas, usa. | chagas disease, caused by trypanosoma cruzi, is a major neglected tropical disease affecting the americas. the epidemiology of this disease in the united states is incomplete. we report evidence of likely autochthonous vectorborne transmission of t. cruzi and health outcomes in t. cruzi-seropositive blood donors in south central texas, usa. | 2017 | 28221110 |
| virulence of trypanosoma cruzi in açai ( euterpe oleraceae martius) pulp following mild heat treatment. | outbreaks of acute chagas disease (acd) in northern brazil can be caused by the ingestion of unprocessed açai pulp contaminated with trypanosoma cruzi . the aim of this study was to determine the minimum thermal process required to inactivate t. cruzi in açai pulp. trypomastigotes (100,000) of t. cruzi y strain were added to 0.15 m nacl or açai pulp and continuously mixed while being heat treated at 37 to 49°c for up to 1 h. when necessary, parasites were separated from açai pulp by forced sievi ... | 2016 | 28221851 |
| anti-trypanosomal activity of non-peptidic nitrile-based cysteine protease inhibitors. | the cysteine protease cruzipain is considered to be a validated target for therapeutic intervention in the treatment of chagas disease. anti-trypanosomal activity against the cl brener strain of t. cruzi was observed in the 0.1 μm to 1 μm range for three nitrile-based cysteine protease inhibitors based on two scaffolds known to be associated with cathepsin k inhibition. the two compounds showing the greatest potency against the trypanosome were characterized by ec50 values (0.12 μm and 0.25 μm) ... | 2017 | 28222138 |
| parasite control and skeletal myositis in trypanosoma cruzi-infected and exercised rats. | non-pharmacological strategies have been rarely described in the treatment of infectious diseases. although exercise training has been recently incorporated in the clinical management of chagas disease, the rationale basis that supports this indication is poorly understood. thus, we investigated the effect of an aerobic exercise on the parasitism, inflammation and oxidative tissue damage in a murine model of trypanosoma cruzi-induced skeletal myositis. wistar rats were randomized into four group ... | 2017 | 28223068 |
| development and evaluation of a nanoemulsion containing ursolic acid: a promising trypanocidal agent : nanoemulsion with ursolic acid against t. cruzi. | over a hundred years after the discovery of chagas disease, this ailment continues to affect thousands of people. for more than 40 years, only two drugs have been available to treat it. ursolic acid is a naturally occurring terpene that has shown a good trypanocidal action. however, the hydrophobicity of this compound presents a challenge for the development of proper delivery systems. nanostructured systems are a prominent in delivering lipophilic drugs. thus, a nanoemulsion containing ursolic ... | 2017 | 28224391 |
| cynomolgus macaques naturally infected with trypanosoma cruzi-i exhibit an overall mixed pro-inflammatory/modulated cytokine signature characteristic of human chagas disease. | non-human primates have been shown to be useful models for chagas disease. we previously reported that natural t. cruzi infection of cynomolgus macaques triggers clinical features and immunophenotypic changes of peripheral blood leukocytes resembling those observed in human chagas disease. in the present study, we further characterize the cytokine-mediated microenvironment to provide supportive evidence of the utility of cynomolgus macaques as a model for drug development for human chagas diseas ... | 2017 | 28225764 |
| decompensated chagasic heart failure versus non-chagasic heart failure at a tertiary care hospital: clinical characteristics and outcomes. | to evaluate clinical and epidemiological characteristics and clinical outcomes in patients hospitalized with decompensated heart failure (dhf), with a comparison between chagas and non-chagas disease. | 2017 | 28225875 |
| chronic chagas cardiomyopathy: a review of the main pathogenic mechanisms and the efficacy of aetiological treatment following the benznidazole evaluation for interrupting trypanosomiasis (benefit) trial. | chagas cardiomyopathy is the most frequent and most severe manifestation of chronic chagas disease, and is one of the leading causes of morbidity and death in latin america. although the pathogenesis of chagas cardiomyopathy is incompletely understood, it may involve several mechanisms, including parasite-dependent myocardial damage, immune-mediated myocardial injury (induced by the parasite itself and by self-antigens), and microvascular and neurogenic disturbances. in the past three decades, a ... | 2017 | 28225894 |
| chronic chagas cardiomyopathy: a review of the main pathogenic mechanisms and the efficacy of aetiological treatment following the benznidazole evaluation for interrupting trypanosomiasis (benefit) trial. | chagas cardiomyopathy is the most frequent and most severe manifestation of chronic chagas disease, and is one of the leading causes of morbidity and death in latin america. although the pathogenesis of chagas cardiomyopathy is incompletely understood, it may involve several mechanisms, including parasite-dependent myocardial damage, immune-mediated myocardial injury (induced by the parasite itself and by self-antigens), and microvascular and neurogenic disturbances. in the past three decades, a ... | 2017 | 28225900 |
| benznidazole and posaconazole in eliminating parasites in asymptomatic t. cruzi carriers: the stop-chagas trial. | benznidazole is recommended for treatment of chagas infection. effects of combination therapy with benznidazole and posaconazole have not been tested in trypanosoma cruzi carriers. | 2017 | 28231946 |
| a new epoch in antitrypanosomal treatment for chagas disease. | 2017 | 28231947 | |
| inhibition of autolysosome formation in host autophagy by trypanosoma cruzi infection. | autophagy has emerged as an essential component of the defense system against intracellular pathogens. we demonstrated that trypanosoma cruzi, an intracellular protozoan parasite, was not eliminated by the host's autophagic machinery despite exposure to the host cell cytoplasm. puncta of microtubule-associated protein 1 light chain 3 (lc3), an autophagy marker, and lc3-ii, a lipidated form of lc3, were significantly increased after infection with t. cruzi, indicating that the parasite activated ... | 2017 | 28232068 |
| induction of cellular proliferation in a human astrocytoma cell line by a trypanosoma cruzi-derived antigen: a mechanism of pathogenesis? | trypanosoma cruzi can compromise the human central nervous system (cns) during acute infection or reactivation in immune-suppressed hosts. astrocytes have been identified as targets of t. cruzi's cns infection in humans. despite a high degree of parasitism and cellular lysis by t. cruzi in vitro the number of astrocytoma cells did not change when compared to uninfected cultures. this work evaluated cellular proliferation, changes in major histocompatibility complex (mhc) expression as a reflecti ... | 2017 | 28234621 |
| immunization with tc52 or its amino terminal domain adjuvanted with c-di-amp induces th17+th1 specific immune responses and confers protection against trypanosoma cruzi. | the development of new adjuvants enables fine modulation of the elicited immune responses. ideally, the use of one or more adjuvants should result in the induction of a protective immune response against the specific pathogen. we have evaluated the immune response and protection against trypanosoma cruzi infection in mice vaccinated with recombinant tc52 or its n- and c-terminal domains (ntc52 and ctc52) adjuvanted either with the sting (stimulator of interferon genes) agonist cyclic di-amp (c-d ... | 2017 | 28234897 |
| rhenium(i) tricarbonyl compounds of bioactive thiosemicarbazones: synthesis, characterization and activity against trypanosoma cruzi. | american trypanosomiasis is a chronic infection discovered and described in 1909 by the brazilian scientist carlos chagas. it is caused by the protozoan parasite trypanosoma cruzi. although it affects about 10million people in latin america, the current chemotherapy is still inadequate. the discovery of new drugs is urgently needed. our group is focused on the development of prospective metal-based drugs mainly based on bioactive ligands and pharmacologically interesting metal ions. in this work ... | 2017 | 28237731 |
| anti-trypanosomatid drug discovery: an ongoing challenge and a continuing need. | the who recognizes human african trypanosomiasis, chagas disease and the leishmaniases as neglected tropical diseases. these diseases are caused by parasitic trypanosomatids and range in severity from mild and self-curing to near invariably fatal. public health advances have substantially decreased the effect of these diseases in recent decades but alone will not eliminate them. in this review, we discuss why new drugs against trypanosomatids are required, approaches that are under investigation ... | 2017 | 28239154 |
| recently differentiated epimastigotes from trypanosoma cruzi are infective to the mammalian host. | trypanosoma cruzi, the etiologic agent of chagas disease, has a complex life cycle in which four distinct developmental forms alternate between the insect vector and the mammalian host. it is assumed that replicating epimastigotes present in the insect gut are not infective to mammalian host, a paradigm corroborated by the widely acknowledged fact that only this stage is susceptible to the complement system. in the present work, we establish a t. cruzi in vitro and in vivo epimastigogenesis mode ... | 2017 | 28240790 |
| desing and synthesis of potent anti-trypanosoma cruzi agents new thiazoles derivatives which induce apoptotic parasite death. | chagas disease, caused by the kinetoplastid protozoan parasite trypanosoma cruzi, remains a relevant cause of illness and premature death and it is estimated that 6 million to 7 million people are infected worldwide. although chemotherapy options are limited presenting serious problems, such as low efficacy and high toxicity. t. cruzi is susceptible to thiazoles, making this class of compounds appealing for drug development. previously, thiazoles resulted in an increase in anti-t. cruzi activity ... | 2017 | 28242550 |
| nitrotriazole-based compounds as antichagasic agents in a long-treatment in vivo assay. | 3-nitrotriazole-based compounds belonging to various chemical subclasses were found to be very effective against chagas disease both in vitro and in vivo after a short administration schedule. in this study, five compounds with specific characteristics were selected to be administered for longer periods of time to mice infected with the virulent trypanosoma cruzi y strain to further evaluate their effectiveness as antichagasic agents and whether or not potential adverse effects occur. benznidazo ... | 2017 | 28242662 |
| nuclear compartmentalization contributes to stage-specific gene expression control in trypanosoma cruzi. | in the protozoan parasite trypanosoma cruzi, as in other trypanosomatids, transcription of protein coding genes occurs in a constitutive fashion, producing large polycistronic transcription units. these units are composed of non-functionally related genes which are pervasively processed to yield each mrna. therefore, post-transcriptional processes are crucial to regulate gene expression. considering that nuclear compartmentalization could contribute to gene expression regulation, we comparativel ... | 2017 | 28243589 |
| endocrine immunology of chagas disease. | the concept of immunoendocrine interactions, existing in normal and pathological conditions, is relatively recent. accordingly, cells from the immune system and from endocrine glands share common receptors for cytokines and hormones, allowing systemic and local regulatory mechanisms. in this context, lymphoid organs are under physiological hormonal control. disturbances in these systems, as those caused by pathogens changes the physiological profile of these interactions, with the release of pro ... | 2017 | 28245460 |
| evaluation of the antichagasic activity of batroxicidin, a cathelicidin-related antimicrobial peptide found in bothrops atrox venom gland. | antimicrobial peptides (amps) are potential alternatives to conventional antibiotics, as they have a fast mode of action, a low likelihood of resistance development and can act in conjunction with existing drug regimens. we report in this study the effects of batroxicidin (batxc), a cathelicidin-related amp from bothrops atrox venom gland, over trypanosoma cruzi, a protozoan that causes chagas' disease. batxc inhibited all t. cruzi (y strain: benznidazole-resistant) developmental forms, with sel ... | 2017 | 28246023 |
| a novel stage-specific glycosomal nucleoside diphosphate kinase from trypanosoma cruzi. | nucleoside diphosphate kinases (ndpk) are key enzymes involved in the intracellular nucleotide maintenance in all living organisms, especially in trypanosomatids which are unable to synthesise purines de novo. four putative ndpk isoforms were identified in the trypanosoma cruzi chagas, 1909 genome but only two of them were characterised so far. in this work, we studied a novel isoform from t. cruzi called tcndpk3. this enzyme presents an atypical n-terminal extension similar to the dm10 domains. ... | 2017 | 28246372 |
| cost-effectiveness of chagas disease screening in latin american migrants at primary health-care centres in europe: a markov model analysis. | chagas disease is currently prevalent in european countries hosting large communities from latin america. whether asymptomatic individuals at risk of chagas disease living in europe should be screened and treated accordingly is unclear. we performed an economic evaluation of systematic chagas disease screening of the latin american population attending primary care centres in europe. | 2017 | 28256340 |
| chagas disease in non-endemic countries. | 2017 | 28256341 | |
| correction to in vitro and in vivo anti-trypanosoma cruzi activity of new arylamine mannich base-type derivatives. | 2017 | 28257207 | |
| antiparasitic treatment induces an improved cd8(+) t cell response in chronic chagasic patients. | chagas disease is a chronic infection caused by trypanosoma cruzi, an intracellular protozoan parasite. chronic chagasic patients (ccps) have dysfunctional cd8(+) t cells that are characterized by impaired cytokine production, high coexpression of inhibitory receptors, and advanced cellular differentiation. most patients diagnosed in the chronic phase of chagas disease already exhibit heart involvement, and there is no vaccination that protects against the disease. antiparasitic treatment is con ... | 2017 | 28258194 |
| differences in the detection of brdu/edu incorporation assays alter the calculation for g1, s, and g2 phases of the cell cycle in trypanosomatids. | trypanosomatids are the etiologic agents of various infectious diseases in humans. they diverged early during eukaryotic evolution and have attracted attention as peculiar models for evolutionary and comparative studies. here, we show a meticulous study comparing the incorporation and detection of the thymidine analogs brdu and edu in leishmania amazonensis, trypanosoma brucei, and trypanosoma cruzi to monitor their dna replication. we used brdu- and edu-incorporated parasites with the respectiv ... | 2017 | 28258618 |
| seroprevalence of five neglected parasitic diseases among immigrants accessing five infectious and tropical diseases units in italy: a cross-sectional study. | this multicentre cross-sectional study aims to estimate the prevalence of five neglected tropical diseases (chagas disease, filariasis, schistosomiasis, strongyloidiasis and toxocariasis) among immigrants accessing health care facilities in five italian cities (bologna, brescia, florence, rome, verona). | 2017 | 28259548 |
| implementing a vector surveillance-response system for chagas disease control: a 4-year field trial in nicaragua. | chagas disease is one of the neglected tropical diseases (ntds). international goals for its control involve elimination of vector-borne transmission. central american countries face challenges in establishing sustainable vector control programmes, since the main vector, triatoma dimidiata, cannot be eliminated. in 2012, the ministry of health in nicaragua started a field test of a vector surveillance-response system to control domestic vector infestation. this paper reports the main findings fr ... | 2017 | 28260529 |
| abnormal 18f-fdg and 82rb pet findings in chagas heart disease. | uptake of the radiopharmaceutical f-fdg visualized by pet imaging can reflect abnormal myocardial inflammation. when utilized in conjunction with other imaging modalities, such as echocardiography, pet f-fdg imaging can help distinguish between active cardiac sarcoidosis and other etiologies of nonischemic cardiomyopathy. we present a case of a 46-year-old man with nonischemic cardiomyopathy and ventricular tachycardia who underwent an echocardiogram suggestive of cardiac chagas disease. a subse ... | 2017 | 28263213 |
| antiprotozoal activity of triazole derivatives of dehydroabietic acid and oleanolic acid. | tropical parasitic diseases such as chagas disease and leishmaniasis are considered a major public health problem affecting hundreds of millions of people worldwide. as the drugs currently used to treat these diseases have several disadvantages and side effects, there is an urgent need for new drugs with better selectivity and less toxicity. structural modifications of naturally occurring and synthetic compounds using click chemistry have enabled access to derivatives with promising antiparasiti ... | 2017 | 28264505 |
| sudden death circadian rhythm in chagasic patients compared to non-chagasic patients. | chagas disease (ch) affects 8-10 million people in latin america, most of them are poor. sudden death (sd) is the major cause of death in patients with ch. to the best of our knowledge, the present report covers the largest reported series comparing the sd of ch versus non-ch patients objective: to compare the circadian rhythm of sd in ch versus non-ch patients. | 2017 | 28267364 |
| acute chagas disease in the brazilian amazon: epidemiological and clinical features. | 2017 | 28268086 | |
| s. mansoni-t. cruzi co-infection modulates arginase-1/inos expression, liver and heart disease in mice. | although schistosoma species and trypanosoma cruzi share common endemic areas, co-infections by these parasites remains overlooked. by using a murine model of s. mansoni and t. cruzi co-infection, we investigated if and to what extent these infections might interact to change the pathological outcomes typically observed when the host is infected by a single parasite species. swiss mice were randomized into four groups: uninfected (ni) and those infected by s. mansoni (sm), t. cruzi (tc) or co-in ... | 2017 | 28268114 |
| different genotypes of trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection. | congenital infection of trypanosoma cruzi allows transmission of this parasite through generations. despite the problematic that this entails, little is known about the placenta environment genetic response produced against infection. we performed functional genomics by microarray analysis in c57bl/6j mice comparing placentas from uninfected animals and from animals infected with two different t. cruzi strains: k98, a clone of the non-lethal myotropic ca-i strain (tci), and vd (tcvi), isolated f ... | 2017 | 28273076 |
| accuracy of chimeric proteins in the serological diagnosis of chronic chagas disease - a phase ii study. | the performance of current serologic tests for diagnosing chronic chagas disease (cd) is highly variable. the search for new diagnostic markers has been a constant challenge for improving accuracy and reducing the number of inconclusive results. | 2017 | 28273127 |
| molecular identification of wild triatomines of the genus rhodnius in the bolivian amazon: strategy and current difficulties. | the amazon region has recently been considered as endemic in latin america. in bolivia, the vast amazon region is undergoing considerable human migrations and substantial anthropization of the environment, potentially renewing the danger of establishing the transmission of chagas disease. the cases of human oral contamination occurring in 2010 in the town of guayaramerín provided reasons to intensify research. as a result, the goal of this study was to characterize the species of sylvatic triato ... | 2017 | 28274885 |
| transcriptome and functional genomics reveal the participation of adenine phosphoribosyltransferase in trypanosoma cruzi resistance to benznidazole. | currently, the only available treatments for trypanosoma cruzi are benznidazole (bz) and nifurtimox (nfx). the mechanisms of action and resistance to these drugs in this parasite are not complete known. in order to identify differentially expressed transcripts between sensitive and resistant parasites, a massive pyrosequencing of the t. cruzi transcriptome was carried out. additionally, the 2d gel electrophoresis profile of sensitive and resistant parasites was analyzed and the data were support ... | 2017 | 28276600 |
| the role of interleukin 17-mediated immune response in chagas disease: high level is correlated with better left ventricular function. | interleukin 17a (il-17a) has been associated with protective rather than pathogenic response in chagas disease (chd). however, it is not established whether or not il-17a-mediated immune response is correlated with patient's left ventricular (lv) function in chd. to address this question we have gathered cardiac functional parameters from chd patients and analysed the possible relationship between their plasma il-17a levels and lv function. plasma il-17a levels were measured by bd cytometric bea ... | 2017 | 28278264 |
| comparison and validation of two computational models of chagas disease: a thirty year perspective from venezuela. | mathematical models can help aid public health responses to chagas disease. models are typically developed to fulfill a particular need, and comparing outputs from different models addressing the same question can help identify the strengths and weaknesses of the models in answering particular questions, such as those for achieving the 2020 goals for chagas disease. | 2017 | 28279459 |
| [history of the department of neurology at the university of buenos aires (1887-2007)]. | in 1887, only five years after jean-martin charcot was awarded the head of neurology at "la salpetrière" in paris, josé maría ramos mejía became the first professor of neurology in south america, at the school of medicine of the university of buenos aires. ramos mejía convoked three assistants, the neuropathologist christofredo jakob, the clinician josé a. esteves and josé ingenieros. hence it followed that neurology in argentina took a stand based on a clinical neurology-neuropathology approach ... | 2016 | 28282088 |
| right ventricular systolic dysfunction in chagas disease defined by speckle-tracking echocardiography: a comparative study with cardiac magnetic resonance imaging. | chagas disease leads to biventricular heart failure, usually with prominent systemic congestion. although echocardiography is widely used in clinical routine, the utility of echocardiographic parameters to detect right ventricular (rv) systolic dysfunction in patients with chagas disease is unknown. we sought to study the diagnostic value of echocardiography, including speckle-tracking parameters, to distinguish individuals with rv systolic dysfunction from those with normal rv systolic function ... | 2017 | 28284461 |
| heart transplantation for chagas cardiomyopathy. | chagas cardiomyopathy (cc) is one of the chronic manifestations of trypanosoma cruzi (t. cruzi) infection and is a major public health disease in latin america. since it is a chronic systemic infection, chagas disease was long considered a potential contraindication for transplantation because of the risk of recurrence with immunosuppression. however, early south american experience in the 1980's established the feasibility of heart transplantation (ht) in patients with chagas disease. indeed, t ... | 2017 | 28284779 |
| synthesis and activity of nucleoside-based antiprotozoan compounds. | parasitic protozoa employ a salvage pathway to synthesize purines and generate essential active nucleotides, whereas mammals are capable of their de novo biosynthesis. this difference provides opportunity for the design of potential new antiprotozoan compounds. a series of 47 adenosine analogues was prepared with modifications at the 2-, 6- and 5'-positions, based on the hypothesis that such compounds would serve as substrates for protozoan nucleoside salvage enzymes, while remaining refractory ... | 2017 | 28284860 |
| cardiac manifestations of parasitic diseases. | the heart may be affected directly or indirectly by a variety of protozoa and helminths. this involvement may manifest in different ways, but the syndromes resulting from impairment of the myocardium and pericardium are the most frequent. the myocardium may be invaded by parasites that trigger local inflammatory response with subsequent myocarditis or cardiomyopathy, as occurs in chagas disease, african trypanosomiasis, toxoplasmosis, trichinellosis and infection with free-living amoebae. in amo ... | 2017 | 28285268 |
| purinergic ecto-enzymes participate in the thromboregulation in acute in mice infection by trypanosoma cruzi. | coagulation disorders have been described in chagas disease with thrombocytopenia as an important event. several mechanisms may be related to this pathogenesis, such as enzymes of the purinergic system, purine, and receptors involved in the regulation and modulation of physiological events related to hemostasis. therefore, the aim of this study was to evaluate the activities of e-ntpdase, e-5'nucleotidase, and ecto-adenosine deaminase (e-ada) in platelets of mice experimentally infected by trypa ... | 2017 | 28285362 |
| differential effects of two widely used solvents, dmso and ethanol, on the growth and recovery of trypanosoma cruzi epimastigotes in culture. | trypanosoma cruzi is the etiological agent of chagas disease. epimastigote forms of t. cruzi can be readily cultured in axenic conditions. ethanol and dimethyl sulfoxide (dmso) are commonly used solvents employed as vehicles for hydrophobic compounds. in order to produce a reference plot of solvent dependent growth inhibition for t. cruzi research, the growth of epimastigotes was analyzed in the presence of different concentrations of ethanol (0.1-4.0%) and dmso (0.5-7.5%). the ability of the pa ... | 2017 | 28285511 |
| chagas disease: an important cause of megaesophagus in latin america. | 2017 | 28285728 | |
| transcriptomic analysis reveals metabolic switches and surface remodeling as key processes for stage transition in trypanosoma cruzi. | american trypanosomiasis is a chronic and endemic disease which affects millions of people. trypanosoma cruzi, its causative agent, has a life cycle that involves complex morphological and functional transitions, as well as a variety of environmental conditions. this requires a tight regulation of gene expression, which is achieved mainly by post-transcriptional regulation. in this work we conducted an rnaseq analysis of the three major life cycle stages of t. cruzi: amastigotes, epimastigotes a ... | 2017 | 28286708 |
| cord blood sample screening for evidence of maternal chagas disease. | 2017 | 28287373 | |
| evaluation of right ventricular systolic function in chagas disease using cardiac magnetic resonance imaging. | right ventricular (rv) impairment is postulated to be responsible for prominent systemic congestion in chagas disease. however, occurrence of primary rv dysfunction in chagas disease remains controversial. we aimed to study rv systolic function in patients with chagas disease using cardiac magnetic resonance. | 2017 | 28289020 |
| prevalence of right ventricular dysfunction in chagas disease: does this depend on the method used? usefulness of cardiac magnetic resonance. | 2017 | 28289021 | |
| challenges in the chemotherapy of chagas disease: looking for possibilities related to the differences and similarities between the parasite and host. | almost 110 years after the first studies by dr. carlos chagas describing an infectious disease that was named for him, chagas disease remains a neglected illness and a death sentence for infected people in poor countries. this short review highlights the enormous need for new studies aimed at the development of novel and more specific drugs to treat chagasic patients. the primary tool for facing this challenge is deep knowledge about the similarities and differences between the parasite and its ... | 2017 | 28289519 |
| [role of autoantibodies against 1-adrenergic receptor in cardiovascular disease]. | according to current knowledge, autoantibodies against 1-adrenergic receptors may be involved in pathogenesis of different cardiovascular diseases and are mostly studied in patients with chagas disease, dilated cardiomyopathy and heart rhythm disorders. they may play an important role in cardiomyocyte apoptosis, alteration of their chrono- and inotropic effects and electrophysiological characteristics. their effects are transduced via 1-adrenergic receptors and depend on multiple factors as liga ... | 2016 | 28290809 |
| immune complexes in chronic chagas disease patients are formed by exovesicles from trypanosoma cruzi carrying the conserved masp n-terminal region. | the exovesicles (evs) are involved in pathologic host-parasite immune associations and have been recently used as biomarkers for diagnosis of infectious diseases. the release of evs by trypanosoma cruzi, the causative agent of chagas disease, has recently been described, with different protein cargoes including the masp multigene family of proteins masps are specific to this parasite and characterized by a conserved c-terminal (c-term) region and an n-terminal codifying for a signal peptide (sp) ... | 2017 | 28294160 |
| frequency of trypanosoma cruzi parasitemia among infected blood donors with a potential association between parasite lineage and transfusion transmission. | trypanosoma cruzi is endemic to the americas where it demonstrates multiple lineages over a vast geographic range (i.e., united states to argentina). these lineages possess divergent geographic and biologic characteristics, including variations in disease manifestations. herein, we report the frequency of parasitemia among seropositive us blood donors and the potential association between parasite lineage and transfusion transmission. | 2017 | 28295355 |
| computer-aided quantification of microvascular networks: application to alterations due to pathological angiogenesis in the hamster. | angiogenesis is both a physiological and a pathological process of great complexity, which is difficult to measure objectively and automatically. the hamster cheek pouch (hcp) prepared for intravital-microscopy (ivm) has been used to characterize microvascular functions in many studies and was chosen to investigate microvascular characteristics observed in normal non-infected hamsters as compared to those hcps parasitized by trypanosoma cruzi. images of hcps captured at ivm were subjected to com ... | 2017 | 28300547 |
| trypanothione reductase: a target for the development of anti-trypanosoma cruzi drugs. | chagas disease or american trypanosomiasis is a major parasitic disease in latin america with treatment available via two drugs: nifurtimox and benznidazole. these two treatments are ineffective in the chronic phase of the disease. therefore, there is a need for the development of new, efficient and safe drugs for the treatment of these diseases. with this goal, one of the promising targets proposed is the trypanothione reductase (tr), a key enzyme important in the metabolism of trypanosoma cruz ... | 2017 | 28302040 |
| clinical trypanosoma cruzi disease after cardiac transplantation in a cynomolgus macaque (macaca fascicularis). | a cynomolgus macaque received a heterotopic cardiac allograft as part of a transplant study, with monoclonal antibodies targeted to specific immune costimulation molecules (cd154, cd28) but no traditional immunosuppressive therapy after surgery. clinical anemia was detected on postoperative day (pod) 35 and had worsened (hgb, 2.3 g/dl; hct = 7.3%) by pod 47, despite type-matched whole-blood transfusions. after a total of 4 blood transfusions, hematologic parameters were improved (hgb, 5.9 g/dl; ... | 2016 | 28304254 |
| trypanocidal effect of isotretinoin through the inhibition of polyamine and amino acid transporters in trypanosoma cruzi. | polyamines are essential compounds to all living organisms and in the specific case of trypanosoma cruzi, the causative agent of chagas disease, they are exclusively obtained through transport processes since this parasite is auxotrophic for polyamines. previous works reported that retinol acetate inhibits leishmania growth and decreases its intracellular polyamine concentration. the present work describes a combined strategy of drug repositioning by virtual screening followed by in vitro assays ... | 2017 | 28306713 |
| mutagenic and cytotoxicity lqb 123 profile: safety and tripanocidal effect of a phenyl-t-butylnitrone derivative. | the therapeutic options for chagas disease are limited and its treatment presents a number of drawbacks including toxicity, drug resistance, and insufficient effectiveness against the chronic stage of the disease. therefore, new therapeutical options are mandatory. in the present work, we evaluated the effect of a phenyl-tert-butylnitrone (pbn) derivate, lqb 123, against trypanosoma cruzi forms. lqb 123 presented a trypanocidal effect against bloodstream trypomastigotes (ic50 = 259.4 ± 6.1 μm) a ... | 2017 | 28316976 |
| pediatric enteric neuropathies: diagnosis and current management. | neurointestinal diseases are increasingly recognized as causes of significant gastrointestinal morbidity in children. this review highlights the most common pediatric enteric neuropathies and their diagnosis and management, emphasizing insights and discoveries from the most recent literature available. | 2017 | 28319561 |
| autoantibodies with beta-adrenergic activity from chronic chagasic patients induce cardiac arrhythmias and early afterdepolarization in a drug-induced lqt2 rabbit hearts. | cardiac arrhythmias are one of the main causes of death in chcp and other dilated cardiomyopathies. previous studies demonstrated that ventricular arrhythmias are associated with the presence of autoantibodies with beta-adrenergic activity, ab-β. | 2017 | 28320606 |
| early polymerase chain reaction detection of chagas disease reactivation in heart transplant patients. | heart transplantation is a valuable therapeutic option for chagas disease patients with severe cardiomyopathy. during patient follow-up, the differential diagnosis between cardiac transplant rejection and chagas disease infection reactivation remains a challenging task, which hinders rapid implementation of the appropriate treatment. herein we investigate whether polymerase chain reaction (pcr) strategies could facilitate early detection of trypanosoma cruzi (t cruzi) in transplanted endomyocard ... | 2017 | 28320630 |
| association of cardiac galectin-3 expression, myocarditis, and fibrosis in chronic chagas disease cardiomyopathy. | chronic chagas disease cardiomyopathy, caused by trypanosoma cruzi infection, is a major cause of heart failure in latin america. galectin-3 (gal-3) has been linked to cardiac remodeling and poor prognosis in heart failure of different etiologies. herein, we investigated the involvement of gal-3 in the disease pathogenesis and its role as a target for disease intervention. gal-3 expression in mouse hearts was evaluated during t. cruzi infection by confocal microscopy and flow cytometry analysis, ... | 2017 | 28322201 |
| mechanistic insights into the anti-angiogenic activity of trypanosoma cruzi protein 21 and its potential impact on the onset of chagasic cardiomyopathy. | chronic chagasic cardiomyopathy (ccc) is arguably the most important form of the chagas disease, caused by the intracellular protozoan trypanosoma cruzi; it is estimated that 10-30% of chronic patients develop this clinical manifestation. the most common and severe form of ccc can be related to ventricular abnormalities, such as heart failure, arrhythmias, heart blocks, thromboembolic events and sudden death. therefore, in this study, we proposed to evaluate the anti-angiogenic activity of a rec ... | 2017 | 28322302 |
| minicircle classes heterogeneity within the tciii and tciv discrete typing units of trypanosoma cruzi. | the taxon trypanosoma cruzi, causative agent of chagas disease, is composed of several discrete typing units (dtus) named tci-tcvi, and tcbat. the history of the taxon t. cruzi is known, even though several controversial aspects remain as the relationships between tciii and tciv. we analyzed cloned t. cruzi stocks pertaining to the seven dtus by filter hybridization tests of pcr amplicons from minicircle variable regions and kinetoplast dna probes. minicircle dna blots from the cloned stocks and ... | 2017 | 28323069 |
| chagas disease diagnostic applications: present knowledge and future steps. | chagas disease, caused by the protozoan trypanosoma cruzi, is a lifelong and debilitating illness of major significance throughout latin america and an emergent threat to global public health. being a neglected disease, the vast majority of chagasic patients have limited access to proper diagnosis and treatment, and there is only a marginal investment into r&d for drug and vaccine development. in this context, identification of novel biomarkers able to transcend the current limits of diagnostic ... | 2017 | 28325368 |
| host-parasite relationships and life histories of trypanosomes in australia. | trypanosomes constitute a group of flagellate protozoan parasites responsible for a number of important, yet neglected, diseases in both humans and livestock. the most significantly studied include the causative agents of african sleeping sickness (trypanosoma brucei) and chagas disease (trypanosoma cruzi) in humans. much of our knowledge about trypanosome host-parasite relationships and life histories has come from these two human pathogens. recent investigations into the diversity and life his ... | 2017 | 28325373 |
| differential cytokine profiling in chagasic patients according to their arrhythmogenic-status. | chagas disease is caused by the protozoan trypanosoma cruzi and is characterized by heart failure and sudden death. identifying which factors are involved in evolution and treatment response is actually challenging. thus, the aim of this work was to determine the th1/th17 (il-6, il-2, tnf, il-17 and ifn-γ) and th2 (il-4 and il-10) serum profile in venezuelan chagasic patients stratified according amiodarone treatment, hypertension and arrhythmias. | 2017 | 28327099 |
| trypanosoma cruzi i genotype among isolates from patients with chronic chagas disease followed at the evandro chagas national institute of infectious diseases (fiocruz, brazil). | trypanosoma cruzi is the etiologic agent of chagas disease in humans, mainly in latin america. trypanosome stocks were isolated by hemoculture from patients followed at evandro chagas national institute of infectious diseases (fiocruz) and studied using different approaches. | 2017 | 28327800 |
| analysis of the seroprevalence of and factors associated with chagas disease in an endemic area in northeastern brazil. | chagas disease (cd) is currently considered a neglected disease; hence, identifying the factors associated with its high prevalence is essential. this study aimed to identify the seroprevalence of and the possible factors associated with cd in inhabitants of the city of limoeiro do norte, northeastern brazil. | 2017 | 28327801 |
| the development of panstrongylus herreri under fluctuating environmental conditions. | panstrongylus herreri is a main chagas disease vector, and its success as a vector stems from its ability to establish domiciliated colonies; we aimed to explore its biology and reproduction. | 2017 | 28327814 |
| adverse systemic reaction to benznidazole. | benznidazole, drug of choice for chagas disease (cd), has been associated with a high incidence of adverse reactions that can become serious, necessitating discontinuation of the drug. we describe the case of a bolivian patient living in spain for 9 years, who, following treatment with benznidazole for cd in indeterminate chronic phase, presented with fever, skin lesions, digestive symptoms, general malaise, and laboratory abnormalities. after the discontinuation of benznidazole and, the intake ... | 2017 | 28327820 |
| ex vivo infection of human placental chorionic villi explants with trypanosoma cruzi and toxoplasma gondii induces different toll-like receptor expression and cytokine/chemokine profiles. | trypanosoma cruzi and toxoplasma gondii present, respectively, low and high congenital transmission rates. the placenta as an immune regulatory organ expresses tlrs, leading to the secretion of cytokines. both parasites are recognized by tlr-2, tlr-4, and tlr-9. here, we studied if the parasites induce differences in tlr protein expression, cytokine profiles, and whether receptor inhibition is related to parasite infection. | 2017 | 28328108 |
| prevalence of chagas disease in the latin american-born population of los angeles. | according to an estimate from the centers for disease control and prevention (cdc), chagas disease (cd) may affect 1.31% of latin american immigrants in the united states, with >300 000 cases. however, there is a lack of real-world data to support this estimate. little is known about the actual prevalence of this neglected tropical disease in the united states, and the bulk of those infected are undiagnosed. | 2017 | 28329123 |
| chagas disease in the united states: out of the shadows. | 2017 | 28329373 | |
| synthesis of novel quinoline-based 4,5-dihydro-1h-pyrazoles as potential anticancer, antifungal, antibacterial and antiprotozoal agents. | a new series of n-substituted 2-pyrazolines 9a-f, 10a-f, 11a-f, 12a-f and 13a-f were obtained from the cyclocondensation reaction of [(7-chloroquinolin-4-yl)amino]chalcones 8a-f with hydrazine hydrate and its derivatives. fourteen of the synthesized compounds including the starting chalcones were selected by us national cancer institute (nci) for testing their anticancer activity against 60 different human cancer cell lines, with the most important gi50 values ranging from 0.28 to 11.7 μm (0.13- ... | 2017 | 28329730 |
| interdisciplinary approach at the primary healthcare level for bolivian immigrants with chagas disease in the city of são paulo. | in a pioneering cross-sectional study among bolivian immigrants in the city of são paulo, brazil, the epidemiological profile, clinical manifestations and morbidity of chagas disease were described. the feasibility of the management of chagas disease at primary healthcare clinics using a biomedical and psychosocial interdisciplinary approach was also tested. previously, a trypanosoma cruzi (t. cruzi) infection rate of 4.4% among 633 immigrants was reported. the samples were screened using two co ... | 2017 | 28333923 |
| performance of tci/tcvi/tcii chagas-flow ate-igg2a for universal and genotype-specific serodiagnosis of trypanosoma cruzi infection. | distinct trypanosoma cruzi genotypes have been considered relevant for patient management and therapeutic response of chagas disease. however, typing strategies for genotype-specific serodiagnosis of chagas disease are still unavailable and requires standardization for practical application. in this study, an innovative tci/tcvi/tcii chagas flow ate-igg2a technique was developed with applicability for universal and genotype-specific diagnosis of t. cruzi infection. for this purpose, the reactivi ... | 2017 | 28333926 |
| antiparasitic lead discovery: toward optimization of a chemotype with activity against multiple protozoan parasites. | human african trypanosomiasis (hat), chagas disease, and leishmaniasis present a significant burden across the developing world. existing therapeutics for these protozoal neglected tropical diseases suffer from severe side effects and toxicity. previously, neu-1045 (3) was identified as a promising lead with cross-pathogen activity, though it possessed poor physicochemical properties. we have designed a library of analogues with improved calculated physicochemical properties built on the quinoli ... | 2017 | 28337329 |
| biodegradable polymeric nanocapsules prevent cardiotoxicity of anti-trypanosomal lychnopholide. | chagas disease is a neglected parasitic disease caused by the protozoan trypanosoma cruzi. new antitrypanosomal options are desirable to prevent complications, including a high rate of cardiomyopathy. recently, a natural substance, lychnopholide, has shown therapeutic potential, especially when encapsulated in biodegradable polymeric nanocapsules. however, little is known regarding possible adverse effects of lychnopholide. here we show that repeated-dose intravenous administration of free lychn ... | 2017 | 28349937 |
| metabolomics profiling reveals a finely tuned, starvation-induced metabolic switch in trypanosoma cruzi epimastigotes. | trypanosoma cruzi, the etiological agent of chagas disease, is a protozoan parasite with a complex lifecycle involving a triatomine insect and mammals. throughout its lifecycle, the t. cruzi parasite faces several alternating events of cell division and cell differentiation in which exponential and stationary growth phases play key biological roles. it is well accepted that arrest of the cell division in the epimastigote stage, both in the midgut of the triatomine insect and in vitro, is require ... | 2017 | 28356355 |
| batf2 inhibits immunopathological th17 responses by suppressing il23a expression during trypanosoma cruzi infection. | inappropriate il-17 responses are implicated in chronic tissue inflammation. il-23 contributes to trypanosoma cruzi-specific il-17 production, but the molecular mechanisms underlying regulation of the il-23-il-17 axis during t. cruzi infection are poorly understood. here, we demonstrate a novel function of batf2 as a negative regulator of il23a in innate immune cells. il-17, but not ifn-γ, was more highly produced by cd4(+) t cells from spleens and livers of t. cruzi-infected batf2(-/-) mice tha ... | 2017 | 28356392 |
| epithelial cell types and their proposed roles in maintaining the mucosal barrier in human chagasic-megacolonic mucosa. | patients suffering from chagasic megacolon must have an intact mucosal barrier as they survive this chronic disease for decades. a key structure of the mucosal barrier are epithelial cells. vasoactive-intestinal-peptide (vip)-positive nerve fibres are involved in influencing, e.g., epithelial cell proliferation, mucus secretion (e.g., mucin 2 and trefoil factor 3 of goblet cells) and inflammation or autoimmunity, all putative and/or known factors altered in chagasic megacolon. we analyzed qualit ... | 2017 | 28357579 |
| antioxidant, antimicrobial, antiparasitic, and cytotoxic properties of various brazilian propolis extracts. | propolis is known for its biological properties and its preparations have been continuously investigated in an attempt to solve the problem of their standardization, an issue that limits the use of propolis in food and pharmaceutical industries. the aim of this study was to evaluate in vitro antioxidant, antimicrobial, antiparasitic, and cytotoxic effects of extracts of red, green, and brown propolis from different regions of brazil, obtained by ethanolic and supercritical extraction methods. we ... | 2017 | 28358806 |
| characterisation of the fumarate hydratase repertoire in trypanosoma cruzi. | nifurtimox and benznidazole represent the only treatments options available targeting chagas disease, the most important parasitic infection in the americas. however, use of these is problematic as they are toxic and ineffective against the more severe stages of the disease. in this work, we used a multidisciplinary approach to characterise the fumarases from trypanosoma cruzi, the causative agent of chagas disease. we showed this trypanosome expresses cytosolic and mitochondrial fumarases that ... | 2017 | 28359888 |
| aryl- or heteroaryl-based hydrazinylphthalazine derivatives as new potential antitrypanosomal agents. | a series of twenty phthalazinyl-hydrazones were synthesized and tested as potential anti-trypanosoma cruzi agents. the phthalazines containing 5-nitroheteroaryl moiety 3l and 3m displayed an excellent in vitro antitrypanosomal profile, exhibiting low micromolar ec50 values against proliferative epimastigote of t. cruzi and minimal toxicity toward vero cells. these derivatives were more potent than the reference drug benznidazole against the epimastigote stage of the parasite. structure-property ... | 2017 | 28359970 |
| inhibitors of pex14 disrupt protein import into glycosomes and kill trypanosoma parasites. | the parasitic protists of the trypanosoma genus infect humans and domestic mammals, causing severe mortality and huge economic losses. the most threatening trypanosomiasis is chagas disease, affecting up to 12 million people in the americas. we report a way to selectively kill trypanosoma by blocking glycosomal/peroxisomal import that depends on the pex14-pex5 protein-protein interaction. we developed small molecules that efficiently disrupt the pex14-pex5 interaction. this results in mislocaliz ... | 2017 | 28360328 |
| heme modulates trypanosoma cruzi bioenergetics inducing mitochondrial ros production. | trypanosoma cruzi is the causative agent of chagas disease and has a single mitochondrion, an organelle responsible for atp production and the main site for the formation of reactive oxygen species (ros). t. cruzi is an obligate intracellular parasite with a complex life cycle that alternates between vertebrate and invertebrate hosts, therefore the development of survival strategies and morphogenetic adaptations to deal with the various environments is mandatory. over the years our group has bee ... | 2017 | 28363600 |
| an in vitro and in vivo evaluation of new potential trans-sialidase inhibitors of trypanosoma cruzi predicted by a computational drug repositioning method. | chagas disease is one of the most important neglected parasitic diseases afflicting developed and undeveloped countries. there are currently limited options for inexpensive and secure pharmacological treatment. in this study, we employed a structure-based virtual screening protocol for 3180 fda-approved drugs for repositioning of them as potential trans-sialidase inhibitors. in vitro and in vivo evaluations were performed for the selected drugs against trypomastigotes from the inc-5 and ninoa st ... | 2017 | 28364659 |
| ictv virus taxonomy profile: dicistroviridae. | dicistroviridae is a family of small non-enveloped viruses with monopartite, linear, positive-sense rna genomes of approximately 8-10 kb. viruses of all classified species infect arthropod hosts, with some having devastating economic consequences, such as acute bee paralysis virus in domesticated honeybees and taura syndrome virus in shrimp farming. conversely, the host specificity and other desirable traits exhibited by several members of this group make them potential natural enemies for inten ... | 2017 | 28366189 |
| simple dialkyl pyrazole-3,5-dicarboxylates show in vitro and in vivo activity against disease-causing trypanosomatids. | the synthesis and antiprotozoal activity of some simple dialkyl pyrazole-3,5-dicarboxylates (compounds 2-6) and their sodium salts (pyrazolates) (compounds 7-9) against trypanosoma cruzi, leishmania infantum and leishmania braziliensis are reported. in most cases the studied compounds showed, especially against the clinically significant amastigote forms, in vitro activities higher than those of the reference drugs (benznidazole for t. cruzi and glucantime for leishmania spp.); furthermore, the ... | 2017 | 28367781 |
| prevalence of trypanosoma cruzi antibodies in blood donors from the sao paulo state, brazil, between 2012 and 2014. | american tripanosomiasis (chagas disease), the second most neglected disease in the world, is caused by the protozoan parasite trypanosoma cruzi. though natural transmission by insect vectors has been controlled, there is significant risk of t. cruzi transmission by blood transfusion in non-endemic regions, generally due to immigration processes from endemic areas. | 2017 | 28368863 |