Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| radiation enhances suicide gene therapy in radioresistant laryngeal squamous cell carcinoma via activation of a tumor-specific promoter. | radioresistant cells have been shown to correlate with poor outcome after radiotherapy. here, we found that human telomerase reverse transcriptase promoter (htertp) had lower activity in laryngeal squamous carcinomas cells than in radioresistant variant cells. combining radiotherapy with plasmid phtertp-hrp, in which expression of enzyme horseradish peroxidase (hrp) controlled by htertp, resulted in increased apoptosis and necrosis of tumor cells after prodrug indole-3-acetic acid (iaa; converte ... | 2009 | 19375219 |
| amperometric biosensor for hydrogen peroxide based on coimmobilized horseradish peroxidase and methylene green in ormosils matrix with multiwalled carbon nanotubes. | a novel amperometric biosensor for the analytical determination of hydrogen peroxide was developed. the fabrication of the biosensor was based on the coimmobilization of horseradish peroxidase (hrp), methylene green (mg) and multiwalled carbon nanotubes within ormosils; 3-aminopropyltrimethoxysilane (aptmos), 2-(3,4-epoxycyclohexyl)ethyltrimethoxysilane (etmos) and phenyltrimethoxysilane (phtmos). aptmos determined the hydrophilicity/hydrophobicity of the ormosils and phtmos and etmos increased ... | 2009 | 19376341 |
| a novel amperometric biosensor for the detection of nitrophenol. | we report on a novel amperometric biosensor for detecting phenolic compounds based on the co-immobilization of horseradish-peroxidase (hrp) and methylene blue (mb) with chitosan on au-modified tio2 nanotube arrays. the titania nanotube arrays were directly grown on a ti substrate using anodic oxidation first; a gold thin film was then coated onto the tio2 nanotubes by an argon plasma technique. the morphology and composition of the fabricated au-modified tio2 nanotube arrays were characterized b ... | 2009 | 19376350 |
| "rainbow" western blotting. | the ''rainbow western'' method permits detection of multiple antigens on a single protein blot. the procedure utilizes horseradish peroxidase (hrpase)-based detection with both a chemiluminescent and colorimetric substrate. in the ''rainbow western'' procedure four different hrpase-colorimetric substrates that produce black, brown, red, and green colors are employed sequentially for detection and simultaneous display of four different antigens on the same blot. the rainbow western methods have t ... | 2009 | 19378037 |
| multiple antigen detection (mad) western blotting. | a variation of ecl immunodetection method permits sequential detection of multiple antigens (mad) on a single protein blot without stripping previously bound antibodies. because antibody stripping is not involved, immobilized proteins are not lost from the membrane, which permits multiple sequential reprobings of the same membrane with different primary antibodies (> or = 12) and retention of strong signal intensities for all antibody probings. this procedure utilizes horseradish peroxidase (hrp ... | 2009 | 19378084 |
| a brief review of other notable protein detection methods on blots. | several methods have been used for detecting proteins on membranes. these include the use of quantum dot luminescent labels, oxyblot immunochemical detection, polymer immunocomplexes, "coupled" probing approach, in situ renaturation of proteins for detecting enzyme activities in crude or purified preparations, immunochromatographic assay, western-phosphatase assay, and the use of congo red dye, a cosmetic color named alta, pro-q emerald 488 dye, or amine-reactive dye in combination with alkaline ... | 2009 | 19378092 |
| sending secret messages on nitrocellulose membrane and the use of a molecular pen for orientation in ecl membrane assays. | invisible ink and writing secret messages have been part of man's fantasy, having proven useful in clandestine and high sensitivity areas. security inks, made up of invisible materials that give printed images that cannot be photocopied, that can be read only under special environments have become important. an ink formulation based on silicon (iv) 2,3-naphthalocyanine bis(trihexylsilyloxide) as colorant, invisible to the naked eye but infrared readable, has been described earlier. biometric dna ... | 2009 | 19378093 |
| enzymatic activity of surface-immobilized horseradish peroxidase confined to micrometer- to nanometer-scale structures in nanocapillary array membranes. | horseradish peroxidase (hrp) was immobilized on the planar surfaces and inside the cylindrical nanopores of nanocapillary array membranes (ncams) to study how the enzyme-catalyzed oxidation of a fluorigenic substrate, amplex red (ar), to fluorescent resorufin by hydrogen peroxide is influenced by confinement. because ar was also found to be converted to resorufin photolytically at high laser fluences, a modified laser-induced fluorescence protocol was developed to characterize the enzyme-catalyz ... | 2009 | 19381375 |
| high performance immunoassay using immobilized enzyme in nanoporous carbon. | a highly stable immunoassay format was constructed using signal-generating enzyme immobilized in nanoporous carbon. a mesocellular carbon foam, called msu-f-c, was loaded with horseradish peroxidase (hrp), followed by cross-linking of the enzyme using glutaraldehyde (ga) and modification of the surface with anti-human igg through edc/sulfo-nhs chemistry. the resulting msu-f-c/hrp/anti-human igg stably retained immobilized enzymes and antibodies, showing higher thermal stability. the msu-f-c/hrp/ ... | 2009 | 19381386 |
| [catalytic activity and the stability of horseradish peroxidase increase as a result of its incorporation into a polyelectrolyte complex with chitosan]. | the incorporation of horseradish peroxidase into polyelectrolyte complexes with chitosans of different molecular weights (mw 5-150 kda) yielded highly active and stable enzyme preparations. as a result of the selection of optimal conditions for the formation of peroxidase-chitosan complexes, it was found that 0.1% chitosan with a mw of 10 kda had the strongest activatory effect on peroxidase (activation degree, > 70%) in the reaction of o-dianisidine oxidation by hydrogen peroxide. the complex f ... | 2013 | 19382699 |
| high-sensitivity amperometric biosensors based on ferrocene-modified linear poly(ethylenimine). | amperometric biosensors for glucose and hydrogen peroxide have been built by immobilizing glucose oxidase (gox) and horseradish peroxidase (hrp) in cross-linked films of ferrocene-modified linear poly(ethylenimine). at ph 7, the glucose sensors generated limiting catalytic current densities of 1.2 ma/cm2. these current densities are approximately 4 times higher than those with other ferrocene-based redox polymers and are comparable to the highest reported values for osmium-based redox polymers w ... | 2009 | 19382795 |
| hydrogen peroxide sensor based on horseradish peroxidase immobilized nanostructured cerium oxide film. | nanostructured cerium oxide (nanoceo(2)) film deposited onto indium-tin-oxide (ito) glass substrate by solution casting has been used for immobilization of horseradish peroxidase (hrp) via physiosorption technique. x-ray diffraction (xrd), scanning electron microscopy (sem), uv-vis and electrochemical techniques have been utilized for characterization of nanoceo(2)/ito electrode and hrp/nanoceo(2)/ito bioelectrode. the hrp/nanoceo(2)/ito electrode exhibits value of the apparent michaelis-menten ... | 2009 | 19393698 |
| a simple strategy for one-step construction of bienzyme biosensor by in-situ formation of biocomposite film through electrodeposition. | one-step construction of horseradish peroxidase (hrp) and glucose oxidase (god) bienzyme biosensor was established based on a simple and controllable electrodeposition approach by in-situ formation of biocomposite film on electrode. with hydrogen peroxide (h(2)o(2)) instantly generated by god-catalyzed oxidation of glucose, quantitative measurement of glucose could be achieved. in the proposed electrodeposition strategy, concanavalin a (con a) was applied as bifunctional cross-linker for in-situ ... | 2009 | 19395252 |
| the role of heparan sulfate on adhesion of 47 and 51 kda outer membrane proteins of helicobacter pylori to gastric cancer cells. | helicobacter pylori is a common gastrointestinal pathogenic bacterium in humans and the usual preference for the stomach's outer membrane proteins (omps) are antigens involved in the adhesion process. through sds-page and blotting analyses, using horseradish peroxidase-labeled heparan sulfate (hrp-hs) as a probe, we identified h. pylori omps with affinity for heparan sulfate (omp-hs). biotin-streptavidin bacterial-adhesion assay was used to evaluate participation of omp-hs in the adhesion of h. ... | 2009 | 19396245 |
| degradation of glyphosate and other pesticides by ligninolytic enzymes. | the ability of pure manganese peroxidase (mnp), laccase, lignin peroxidase (lip) and horseradish peroxidase (hrp) to degrade the widely used herbicide glyphosate and other pesticides was studied in separate in vitro assays with addition of different mediators. complete degradation of glyphosate was obtained with mnp, mnso4 and tween 80, with or without h2o2. in the presence of mnso4, with or without h(2)o(2), mnp also transformed the herbicide, but to a lower rate. laccase degraded glyphosate in ... | 2009 | 19396551 |
| gold nanoparticle-modified ultramicroelectrode arrays for biosensing: a comparative assessment. | gold ultramicroelectrode arrays (umeas) modified with gold nanoparticles (gnp) are shown to be a highly suitable transducer platform for the fabrication of biosensors. comparative studies were carried out with microelectrodes and umeas, the latter being either bare or modified with gnps. gnps could be electrodeposited on to the umea surface, thereby increasing its active area up to one hundred times but without affecting its inherent electrodic properties. horseradish peroxidase enzyme (hrp) was ... | 2009 | 19401273 |
| designing nanomaterial-enhanced electrochemical immunosensors for cancer biomarker proteins. | detection of multiple cancer biomarker proteins in human serum and tissue at point-of-care is a viable approach for early cancer detection, but presents a major challenge to bioanalytical device development. this article reviews recent approaches developed in our laboratories combining nanoparticle decorated electrodes and multilabeled secondary antibody labeled particles to achieve high sensitivity for the detection of cancer biomarker proteins. two nanomaterial-based sensor platforms were used ... | 2009 | 19403342 |
| linseed oil in the maternal diet during gestation and lactation modifies fatty acid composition, mucosal architecture, and mast cell regulation of the ileal barrier in piglets. | in this study, we investigated the effect of supplementation of the maternal diet with linseed oil [rich in 18:3(n-3)] on fatty acid composition, mucosal architecture, and mast cell regulation of barrier function in piglet ileum. sixteen sows were fed a lard (lar)- or a linseed oil (lso)-based diet during gestation and lactation. fatty acid composition of maternal rbc at parturition and of milk at d 14 of lactation were determined. fatty acid composition, villous-crypt structure, and permeabilit ... | 2009 | 19403717 |
| direct electrochemistry and spectroelectrochemistry of osmium substituted horseradish peroxidase. | in this contribution the substitution of the central protoporphyrin ix iron complex of horseradish peroxidase by the respective osmium porphyrin complex is described. the direct electrochemical reduction of the os containing horseradish peroxidase (oshrp) was achieved at ito and modified glassy carbon electrodes and in combination with spectroscopy revealed the three redox couples os(iii)hrp/os(iv)hrp, os(iv)hrp/os(v)hrp and os(v)hrp/os(vi)hrp. the midpoint potentials differ dependent on the ele ... | 2009 | 19411193 |
| a hybrid biofuel cell based on electrooxidation of glucose using ultra-small silicon nanoparticles. | the ultra-small silicon nanoparticle was shown to be an electrocatalyst for the electrooxidation of glucose. the oxidation appeared to be a first order reaction which involves the transfer of 1 electron. the oxidation potential showed a low onset of -0.4v vs. ag/agcl (-0.62 v vs. rhe). the particle was used as the anode catalyst of a prototype hybrid biofuel cell, which operated on glucose and hydrogen peroxide. the output power of the hybrid cell showed a dependence on the enzymes used as the c ... | 2009 | 19423331 |
| detection of circulating antigen in serum of mice infected with schistosoma japonicum by immunomagnetic bead elisa based on igy. | we developed a novel immunomagnetic bead elisa based on igy (egg yolk immunoglobulin) for detection of circulating antigen (ca) in sera of mice infected with schistosoma japonicum. the assay involved the use of chicken polyclonal antibodies igy against soluble egg antigens (sea) of s. japonicum as a capture antibody and anti-sea mouse monoclonal antibody np28-5b labeled horseradish peroxidase (hrp) as a detecting antibody. two groups of balb/c mice infected with s. japonicum cercariae were used: ... | 2009 | 19426661 |
| quantification of e. coli dna on a flow-through chemiluminescence microarray readout system after pcr amplification. | we report on a hybridization assay using dna microarrays for the quantification of amplification products of the uida gene of e. coli. using the stopped-pcr strategy, the amplified target dna was strongly dependent on the applied gene copies. the quantification was carried out by a flow-through chemiluminescence microarray readout system. the dna microarrays were based on a poly(ethylene glycol)-modified glass substrate. the probes on the surface were 18 or 25 nucleotides long and the quantified ... | 2009 | 19430151 |
| interhemisphere connections of eye dominance columns in the cat visual cortex in conditions of impaired binocular vision. | data from studies of interhemisphere connections in fields 17 and 18 of cats reared in conditions of impaired binocular vision (monocular deprivation, uni- and bilateral strabismus) are presented. monosynaptic connections between neurons were studied by microiontophoretic application of horseradish peroxidase into cortical eye dominance columns and the distributions of retrograde labeled callosal cells were analyzed. spatial asymmetry and eye-specific interhemisphere neuron connections persisted ... | 2009 | 19430981 |
| softening of the packing density of horseradish peroxidase by a h-donor bound near the heme pocket. | we use pressure tuning of spectral holes to estimate the compressibility of protein molecules by optical means. we found that the compressibility of mesoporphyrin-substituted horseradish peroxidase increases by a factor of three when it incorporates small aromatic h-donor molecules that bind in the vicinity of its heme pocket. such a dramatic softening of its packing density corresponds to a jump from a compressibility range characteristic for the solid state into that characteristic for liquids ... | 1992 | 19431865 |
| measuring soluble platelet glycoprotein vi in human plasma by elisa. | recent experimental evidence demonstrates that the platelet-specific collagen receptor, glycoprotein (gp)vi is essentially all uncleaved on normal circulating platelets, but is shed from the platelet surface in a metalloproteinase-dependent manner in response to gpvi ligands (including collagen), anti-gpvi antibodies or activation at the platelet fc receptor, fcgammariia. this raises the question of whether shed ectodomain fragment in plasma could be a useful biomarker of thrombotic risk and/or ... | 2009 | 19437330 |
| homogenous thionine-sio2 nanocomposite spheres: sonochemical preparation, characterization, and application in h2o2 biosensor. | the electrochemical properties of new homogenous thionine (th)-doped silica nanoparticles as a mediator were studied. the nanocomposites were synthesized by using sonochemical method. the sonication induced the strong combination between dye molecules and silica in the construction of the composites. by controlling reaction condition, face rougher uniform spheres could be achieved. the as-prepared nanocomposites can be used as the mediator to construct the biosensors with horseradish peroxidase ... | 2009 | 19437985 |
| nanostructured electrode with titania nanotube arrays: fabrication, electrochemical properties, and applications for biosensing. | titania nanotube (tnt) arrays were fabricated by anodic oxidation of titanium foil in various electrolytes and were calcined in dry nitrogen at various temperatures. the morphology, crystallinity, and chemical composition of tnt arrays were studied by means of x-ray diffraction (xrd) and scanning electron microscopy (sem). electrochemical impedance spectroscopy (eis) was employed to investigate the electrical conductivity and capacitance of tnt arrays prior to and after calcination. the results ... | 2009 | 19437986 |
| characterization of multienzyme-antibody-carbon nanotube bioconjugates for immunosensors. | characterization studies of a multi-enzyme-antibody-carbon nanotube bioconjugate designed for the amplification of electrochemical immunosensing are described. secondary antibodies for prostate specific antigen (psa) were covalently linked to highly carboxylated multi-walled carbon nanotube (cnt) along with multiple horseradish peroxidase (hrp) enzyme labels. these bioconjugates provide ultra-sensitive amperometric detection of psa on a single-wall carbon nanotube forest sandwich immunosensor pl ... | 2009 | 19441303 |
| quantification of biogenic amines by microchip electrophoresis with chemiluminescence detection. | a highly sensitive microchip electrophoresis (mce) method with chemiluminescence (cl) detection was developed for the determination of biogenic amines including agmatine (agm), epinephrine (e), dopamine (da), tyramine, and histamine in human urine samples. to achieve a high assay sensitivity, the targeted analytes were pre-column labeled by a cl tagging reagent, n-(4-aminobutyl)-n-ethylisoluminol (abei). abei-tagged biogenic amines after mce separation reacted with hydrogen peroxide in the prese ... | 2009 | 19447398 |
| research on the application of horseradish peroxidase and hydrogen peroxide to the oil removal of oily water. | horseradish peroxidase (hrp) has the advantages of low cost, obtaining convenience, broad substrate specificity, etc, so it may be a suitable kind of enzyme to remove dilute emulsified oil and dissolved oil from water. the effect factors of the oil removal using hrp, such as ph, concentrations of hrp and h2o2, additives, fe2+ ion, temperature and reaction time were researched. when the initial oil concentration was 120 mg/l, the remaining oil concentrations of the synthetic oily water and an act ... | 2009 | 19448310 |
| multi-functional crosslinked au nanoaggregates for the amplified optical dna detection. | we report a novel sandwich type assay for the optical detection of dna using multi-component crosslinked au nanoparticle aggregates. in this design, a dna bridged multi-functional au nanoparticle aggregate, which integrates dna recognition (detection probe), signal amplification (enzyme, horseradish peroxidase) and non-specific blocking (bovine serum albumin, bsa) section, is employed as detection probe. the sandwich type assay includes the capture probe that immobilized on magnetic microparticl ... | 2009 | 19450967 |
| muc1 traverses apical recycling endosomes along the biosynthetic pathway in polarized mdck cells. | muc1 is a heavily glycosylated transmembrane protein localized at the apical surface of polarized epithelial cells. here, we examined the biosynthetic route of newly synthesized muc1 in polarized madin-darby canine kidney (mdck) cells. apically and basolaterally destined cargo are sorted at the trans-golgi network into distinct vesicles, and proteins with lipid raft-dependent apical targeting signals and glycan-dependent apical targeting signals appear to specifically transit apical early endoso ... | 2009 | 19453277 |
| [fluorescence quenching assay of ultratrace horseradish peroxidase using rhodamine dye]. | in acetate buffer solution, the reaction of h2o2 with ki was catalyzed by horseradish peroxidase (hrp) to form i3-. the i3- combined respectively with rhodamine s(rhs), rhodamine 6g(rh6g), rhodamine b(rhb) and butyl-rhodamine b(b-rhb) to form rhs-i3, rh6g-i3, rhb-i3 and b-rhb-i3 association particles, resulting in the fluorescence quenching at 580, 580, 554 and 554 nm, respectively. the effect of ph value, rhodamine dye concentration, ki concentration, h2o2 concentration, reaction temperature an ... | 2009 | 19455817 |
| overlap of nigrothalamic terminals and thalamostriatal neurons in the feline lateralis medialis-suprageniculate nucleus. | the lateralis medialis-suprageniculate nucleus (lm-sg) of the feline posterior thalamus is a relay nucleus with a clear visuomotor function. in this study, we examined the distribution of axon terminals of the nigral afferent to the lm-sg following injection of an anterograde tracer, biocytin, into the substantia nigra pars reticulata, and the distribution of the thalamostriatal projection neurons in the lm-sg following the injection of wheat germ agglutinin conjugated with horseradish peroxidas ... | 2009 | 19457764 |
| extraction of allyl isothiocyanate from horseradish (armoracia rusticana) and its fumigant insecticidal activity on four stored-product pests of paddy. | isothiocyanates (itcs) extracted from armoracia rusticana gaertn., may & scherb. have been shown previously to have insecticidal activity. allyl isothiocyanate (aitc), a major component of itcs with high volatility, was therefore extracted using different methods and tested as a fumigant against four major pest species of stored products, maize weevil sitophilus zeamais (motsch.), lesser grain borer rhizopertha dominica (f.), tribolium ferrugineum (f.) and book louse liposcelis entomophila (ende ... | 2009 | 19459178 |
| enzyme encapsulation in microparticles composed of polymerized ionic liquids for highly active and reusable biocatalysts. | horseradish peroxidase (hrp) is encapsulated in polymerized ionic liquid microparticles (pil-mp), which are prepared by polymerization of 1-vinyl-3-ethylimidazolium bromide in the presence of the crosslinker n,n'-methylenebis(acrylamide) in a concentrated water-in-oil (w/o) emulsion. pil-mp encapsulating hrp chemically-modified with comb-shaped polyethylene glycol (pm(13)-hrp) exhibit excellent activity for guaiacol oxidation in an aqueous solution. the pm(13)-hrp in pil-mp shows more than 2-fol ... | 2009 | 19462045 |
| a rapid and simple method for ultrasensitive electrochemical immunoassay of protein by an electric field-driven strategy. | a sensitive, rapid and simple method for analysis of protein was proposed by introducing an electric field-driven technique to the incubation process of immunoassay and immobilizing horseradish peroxidase (hrp) labeled antibody in a newly designed gel matrix to construct an immunosensor. with an electric field-driven technique, the incubation for immuno-recognition could be completed within 2 min. the immobilized hrp showed excellent direct electrochemistry in this new gel matrix, and the detect ... | 2009 | 19463559 |
| altered functional development of the blood-brain barrier after early life stress in the rat. | this study investigated the effect of early life stress on blood-brain barrier functional development in an altricial mammal, the wistar rat. forced swimming between gestational days 10-20 was used as a stress procedure in pregnant rats. after delivery, half of the control litters underwent 180 min maternal separation from postnatal day 2-20. controls were kept without any stress manipulations. at sacrifice between postnatal days 1-30 subjects were given intracardiac evans blue or the lectin whe ... | 2009 | 19463912 |
| development and validation of an enzyme-linked immunosorbent assay for the quantification of trastuzumab in human serum and plasma. | trastuzumab, a humanized monoclonal antibody, is used for the treatment of breast cancer patients who overexpress the her2 receptor. to optimize therapy, pharmacokinetic studies are necessary. the aim of this study was to develop an enzyme-linked immunosorbent assay (elisa) for trastuzumab to support these pharmacokinetic studies. for this immunoassay, we raised anti-idiotype antibodies in rabbits. after purification of the rabbit material, the anti-idiotype antibodies are used as capturing anti ... | 2009 | 19464994 |
| intestinal physiology and peptidase activity in male pigs are modulated by consumption of corn culture extracts containing fumonisins. | fumonisin b(1) (fb1) alters intestinal epithelial cell cycle and absorptive, secretory, and barrier properties in vitro, but in vivo data are lacking. therefore, we tested the hypothesis that repeated intake of a corn culture extract rich in fumonisins, mainly in fb1, alters indices of intestinal absorptive and secretory physiology and barrier function in vivo. intra-litter pairs of pigs (n = 36) weaned at 28 d, were fed the vehicle (control) or the extract (providing 1.5 mg fb1/kg body weight) ... | 2009 | 19474154 |
| cellular and vascular changes in the retina of neonatal rats after an acute exposure to hypoxia. | this study was undertaken to examine the effects of an acute hypoxic exposure on the retinal cells and production of vascular factors such as vascular endothelial growth factor (vegf) and nitric oxide (no), which may affect vascular permeability in the developing retina. | 2009 | 19474404 |
| light and electron microscopic analysis of the somata and parent axons innervating the rat upper molar and lower incisor pulp. | the morphology of intradental nerve fibers of permanent teeth and of continuously growing rodent incisors has been studied in detail but little information is available on the parent axons that give rise to these fibers. here we examined the axons and somata of trigeminal neurons that innervate the rat upper molar and lower incisor pulp using tracing with horseradish peroxidase and light and electron microscopic analysis. the majority (approximately 80%) of the parent axons in the proximal root ... | 2009 | 19477235 |
| biocatalyzed deposition amplification for detection of aflatoxin b1 based on quartz crystal microbalance. | an ultrasensitive piezoelectric method for the detection of the aflatoxin b1 (afb1) based on the indirect competitive immunoassay and the biocatalyzed deposition amplification has been developed. in this method, the quartz crystal surface was coated with a self-assembled monolayer of 3-mercaptopropionic acid (mpa) for covalently immobilization of the bsa-afb1 conjugate, which could compete with the free afb1 for binding to the anti-afb1 antibody (msigg). after the competitive immunoreaction, the ... | 2009 | 19481636 |
| a quantitative enzyme-linked immunosorbent assay and filtration-based fluorescent antibody test as potential tools to screen broodstock for infection with flavobacterium psychrophilum. | there is strong evidence that flavobacterium psychrophilum, the etiologic agent of coldwater disease, is transmitted vertically; it has been hypothesized that disease management at hatchery facilities can be improved through broodstock screening and implementation of culling programs. this paper describes the development of two assays used to screen broodstock tissues (kidney and ovarian fluid) for the presence of f. psychrophilum. four monoclonal antibodies (mabs) were generated against outer m ... | 2009 | 19485125 |
| poly(methyl metacrylate) conductive fiber optic transducers as dual biosensor platforms. | herein the development of an alternative optic-conductive fiber configuration applied for the construction of biosensing platforms. this new approach is based on applying the chemical polymerization of pyrrole onto the surface of polymethyl metacrylate (pmma) fibers to create a polymer--a conductive surface, onto which an additional photoactive polypyrrole-benzophenone (ppybz) film is electrochemically generated upon the fiber surface. irradiation of the benzophenone groups embedded in the ppy f ... | 2009 | 19487114 |
| serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. | diagnosis of hydatidosis is based on immunodiagnostic methods along with radiological and ultrasound examinations. the objectives of the present study were to develop a specific and simple antigen-based elisa method for diagnosis of hydatidosis and compare it with antibody detection method. the subjects in this study included 89 patients in the following groups: surgically confirmed hydatidosis patients (35 cases), control with other parasitic diseases (29 cases), and healthy controls (25 cases) ... | 2009 | 19488422 |
| electrochemical immunochip sensor for aflatoxin m1 detection. | an investigation into the fabrication, electrochemical characterization, and development of a microelectrode array (mea) immunosensor for aflatoxin m(1) is presented in this paper. gold meas (consisting of 35 microsquare electrodes with 20 microm x 20 microm dimensions and edge-to-edge spacing of 200 microm) together with on-chip reference and counter electrodes were fabricated using standard photolithographic methods. the meas were then characterized by cyclic voltammetry, and the behavior of t ... | 2009 | 19489595 |
| bilateral innervation of syringeal muscles by the hypoglossal nucleus in the jungle crow (corvus macrorhynchos). | bird vocalizations are produced by contractions of syringeal muscles, which are controlled by the hypoglossal nucleus. in oscines, syringeal muscles are controlled by the hypoglossal nucleus ipsilaterally, whereas syringeal innervation is bilateral in non-oscines. we have determined the course of hypoglossal nerves in the jungle crow corvus macrorhynchos. our results indicate a cross-over of the hypoglossal nerve from the left side to the right side on the trachea 7 mm rostral to the musculus st ... | 2009 | 19490396 |
| topographical and quantitative distribution of the projecting neurons to main divisions of the septal area. | septal area is a limbic structure that is involved in the regulation of several autonomic, learning-related and behavioral functions. participation of this area in various physiologic functions is indicative of its extensive connections with different brain areas. it contains two major divisions: lateral septum (ls) and medial septum/diagonal band of broca (ms/dbb). in the present work, we examined topographical distribution of projecting neurons to these divisions and quantitatively verified th ... | 2009 | 19493383 |
| determination of busulfan in human plasma using an elisa format. | high-dose busulfan is an important component of many bone marrow transplantation-preparative regimens. high busulfan plasma levels have been shown to increase the chance of venoocclusive disease and low levels are associated with recurrence of disease or graft rejection. currently, busulfan levels are monitored by physical methods that are expensive and time consuming, resulting in relatively low overall use of busulfan testing for dose adjustment. novel highly selective antibodies for busulfan ... | 2009 | 19494794 |
| dendrobium findleyanum agglutinin: production, localization, anti-fungal activity and gene characterization. | the recently reported dendrobium findleyanum agglutinin (dfa) was identified and determined in different parts of d. findleyanum pseudobulbs by using western blot analysis, lc-ms/ms, sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and histochemical procedure. western blot analysis of crude protein extract with horseradish peroxidase (hrp), a mannose-rich glycoprotein, showed only one band at 14.5 kda, which had the same molecular mass as dfa. this band was a major band when ... | 2009 | 19495769 |
| automated support-resolution strategy for a one-way chemiluminescent multiplex immunoassay. | an automated support-resolution strategy was designed to couple with a flow-through immunosensing system for performing a one-way chemiluminescent (cl) multiplex immunoassay. different from multilabel and multichannel-based detection techniques, this immunoassay method employed a single horseradish peroxidase (hrp) label in one way. with the use of carcinoembryonic antigen (cea) and alpha-fetoprotein (afp) as model analytes, the capture antibodies for cea and afp were immobilized on the inner wa ... | 2009 | 19499927 |
| 3-(10'-phenothiazinyl)propane-1-sulfonate is a potent enhancer of soybean peroxidase-induced chemiluminescence. | 3-(10'-phenothiazinyl)propane-1-sulfonate (sptz) was shown to be a potent enhancer of soybean peroxidase (sbp)-induced chemiluminescence. to the best of our knowledge, sptz is the first enhancer of sbp to be discovered. optimal conditions for sbp-catalyzed oxidation of luminol in the presence of sptz were determined. the sbp-sptz system showed better sensitivity and a lower detection limit (ldl) with respect to the horseradish peroxidase-4-iodophenol system traditionally used in chemiluminescent ... | 2009 | 19500555 |
| west nile virus preferentially transports along motor neuron axons after sciatic nerve injection of hamsters. | prior findings led us to hypothesize that west nile virus (wnv) preferentially transports along motor axons instead of sensory axons. wnv is known to undergo axonal transport in cell culture and in infected hamsters to infect motor neurons in the spinal cord. to investigate this hypothesis, wnv was injected directly into the left sciatic nerve of hamsters. wnv envelope-staining in these hamsters was only observed in motor neurons of the ipsilateral ventral horn of the spinal cord, but not in the ... | 2009 | 19504391 |
| bioactivation of minocycline to reactive intermediates by myeloperoxidase, horseradish peroxidase, and hepatic microsomes: implications for minocycline-induced lupus and hepatitis. | of the tetracyclines, minocycline is unique in causing a significant incidence of a lupus-like syndrome and autoimmune hepatitis. it is also unique among the tetracyclines in having a para-n,n-dimethylaminophenol ring. many drugs that cause autoimmune reactions are oxidized to reactive metabolites by the myeloperoxidase (mpo) system of macrophages. in this study, we showed that minocycline is oxidized to reactive intermediates by mpo/h(2)o(2)/cl(-), hocl, horseradish peroxidase/h(2)o(2), or hepa ... | 2009 | 19505990 |
| substrate specificity of myriococcum thermophilum cellobiose dehydrogenase on mono-, oligo-, and polysaccharides related to in situ production of h2o2. | cellobiose dehydrogenase from the ascomycete fungus myriococcum thermophilum (mtcdh) was tested for the ability to generate bleaching species at a ph suitable for liquid detergents. the catalytic properties of mtcdh were investigated for a large variety of carbohydrate substrates using oxygen as an electron receptor. mtcdh produces h2o2 with all substrates tested (except fructose) but only in the presence of a chelant. insoluble substrates like cellulose and cotton could as well be oxidized by m ... | 2009 | 19506859 |
| logic gates and antisense dna devices operating on a translator nucleic acid scaffold. | a series of logic gates, "and", "or", and "xor", are designed using a dna scaffold that includes four "footholds" on which the logic operations are activated. two of the footholds represent input-recognition strands, and these are blocked by complementary nucleic acids, whereas the other two footholds are blocked by nucleic acids that include the horseradish peroxidase (hrp)-mimicking dnazyme sequence. the logic gates are activated by either nucleic acid inputs that hybridize to the respective " ... | 2009 | 19507821 |
| high throughput heme assay by detection of chemiluminescence of reconstituted horseradish peroxidase. | in living organisms, heme is an essential molecule for various biological functions. recent studies also suggest that heme functions as organelle-derived signal that regulates fundamental cell processes. furthermore, estimation of heme is widely used for studying various blood disorders. in this regard, development of a rapid, sensitive, and high throughput heme assay has been sought. the most frequently used method of measuring heme by pyridine hemochrome is time, labor, and material intensive, ... | 2009 | 19519333 |
| multiple reprobing of western blots after inactivation of peroxidase activity by its substrate, hydrogen peroxide. | sequential detections of different proteins on western blot save time and precious samples. the main problem concerning reprobing is that stripping buffers can unbind both the antibody and the tested antigen. an original reprobing method has been set up based on horseradish peroxidase (hrp) inhibition after enhanced chemiluminescence detection. instead of removing previously fixed antibodies as common stripping buffers do, the hrp activity linked to the secondary antibody is irreversibly inhibit ... | 2009 | 19523435 |
| enzyme logic gates for the digital analysis of physiological level upon injury. | a biocomputing system composed of a combination of and/identity logic gates based on the concerted operation of three enzymes: lactate oxidase, horseradish peroxidase and glucose dehydrogenase was designed to process biochemical information related to pathophysiological conditions originating from various injuries. three biochemical markers: lactate, norepinephrine and glucose were applied as input signals to activate the enzyme logic system. physiologically normal concentrations of the markers ... | 2009 | 19523809 |
| the bioanalysis of trastuzumab in human serum using precipitate-enhanced ellipsometry. | for the bioanalysis of therapeutic monoclonal antibodies in biological matrices, immunoassays--especially enzyme-linked immunosorbent assays (elisas)--are the most widely used techniques. although elisas are very sensitive, the obtained sensitivity is not always sufficient. in this study, we have investigated the possibilities of performing a precipitate-enhanced immunoassay (peia) with ellipsometric detection for the bioanalysis of the therapeutic monoclonal antibody trastuzumab. hydrophobic si ... | 2009 | 19523916 |
| agonistic behavior enhances adult neurogenesis in male acheta domesticus crickets. | we examined the effect of agonistic behavior on cell proliferation and neurogenesis in the central nervous system (cns) of adult male acheta domesticus crickets. we combined 5-bromo,2'deoxyuridine (brdu)-labeling of dividing cells with immunocytochemical detection of the neuronal marker horseradish peroxidase to examine the proliferation of progenitor cells and the survival of newborn neurons. in crickets, the mushroom bodies of the brain contain clusters of proliferative cells that divide and g ... | 2009 | 19525431 |
| chemiluminescent determination of h2o2 using 4-(1,2,4-triazol-1-yl)phenol as an enhancer based on the immobilization of horseradish peroxidase onto magnetic beads. | this article describes the employment of a novel p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (trp), as a highly potent signal enhancer of the luminol-hydrogen peroxide (h(2)o(2))-horseradish peroxidase (hrp) chemiluminescence (cl) system. the cl reaction conditions were optimized, and the enhancement characteristics of trp were compared with those of p-iodophenol (pip). trp produced a strong enhancement of the cl with the effect of prolonging the light emission. the developed system was th ... | 2009 | 19527675 |
| prevention of recurrent urinary tract infection in children. | urinary tract infection (uti) in children is common (5-10%) and recurs in 10-30%. uti causes an unpleasant, usually febrile illness in children. this review focuses on studies evaluating interventions to prevent uti in children and published between january 2007 and june 2008. | 2009 | 19532083 |
| dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy. | this paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (tir-fcs) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. objective-type tir-fcs combines the advantages of fcs with tirf microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging. | 2007 | 19532790 |
| embryonic development of pleuropodia of the cicada, magicicada cassini. | in many insects the first abdominal segment possesses embryonic appendages called pleuropodia. here we show the embryogenesis of pleuropodial cells of the periodical cicada, magicicada cassini (fisher 1851) (insecta, homoptera, cicadidae). an antibody, anti-horseradish perioxidase (hrp), that is usually neuron-specific strongly marked the pleuropodial anlagen and revealed their ectodermal origin shortly after limb bud formation. thereafter the cells sank into the epidermis and their apical parts ... | 2006 | 19537987 |
| enzyme-modified nanoparticles using biomimetically synthesized silica. | the entrapment of enzymes within biomimetic silica nanoparticles offers unique and simple immobilization protocols that merge the stability of proteins confined in solid phases with the high loading and reduced diffusion limitations inherent to nano-sized structures. herein, we report on the biomimetic silica entrapment of chemically derivatized horseradish peroxidase for amperometric sensing applications. scanning electron microscopy shows evidence of the formation of enzyme-modified nanosphere ... | 2009 | 19540173 |
| automated determination of asulam by enhanced chemiluminescence using luminol/peroxidase system. | a flow injection system with chemiluminescence detection for the determination of asulam, enhancer of the system luminol-h(2)o(2)-horseradish peroxidase, is proposed. the method shows a moderate selectivity against other pesticides usually present in formulations of herbicides and in water. the procedure was applied to the determination of asulam in tap water samples and a recovery study was carried out in order to validate the method. the obtained results show acceptable recovery values (betwee ... | 2013 | 19544320 |
| development and technical and clinical validation of a quantitative enzyme-linked immunosorbent assay for the detection of human antibodies to hepatitis b surface antigen in recipients of recombinant hepatitis b virus vaccine. | pending removal from the market of a commercial assay (the ausab [abbott laboratories] enzyme immunoassay [eia]) for the determination of antibodies to hepatitis b surface antigen (hbsag), a new in-house quantitative enzyme-linked immunosorbent assay (elisa) to measure antibodies against hbsag (anti-hbs) was developed (anti-hbs in-house). specific anti-hbs antibodies were sandwiched between the precoated hbsag ad and ay subtypes purified from plasma from hepatitis b virus (hbv) human carriers an ... | 2009 | 19553553 |
| alterations in blood-brain barrier permeability to large and small molecules and leukocyte accumulation after traumatic brain injury: effects of post-traumatic hypothermia. | we investigated the temporal and regional profile of blood-brain barrier (bbb) permeability to both large and small molecules after moderate fluid percussion (fp) brain injury in rats and determined the effects of post-traumatic modest hypothermia (33 degrees c/4 h) on these vascular perturbations. the visible tracers biotin-dextrin-amine 3000 (bda-3k, 3 kda) and horseradish peroxidase (hrp, 44 kda) were injected intravenously at 4 h or 3 or 7 days post-tbi. at 30 min after the tracer infusion, ... | 2009 | 19558276 |
| identification of (l)-fucose-binding proteins from the nile tilapia (oreochromis niloticus l.) serum. | lectins are carbohydrate-binding proteins with many biological functions including cellular recognition and innate immunity. in this study, a major l-fucose-binding lectin from the serum of nile tilapia (oreochromis niloticus l.), designated as tfbp, was isolated by l-fucose-bsa sepharose cl6b affinity chromatography. the sds-page (10%) analysis of tfbp revealed a major band of approximately 23 kda with an n-terminal amino acid sequence of dqtetagqqsxpqdihavlrel which did not give significant si ... | 2009 | 19563899 |
| effects of substrate, protein environment, and proximal ligand mutation on compound i and compound 0 of chloroperoxidase. | this paper investigates the enzyme chloroperoxidase (cpo) by means of hybrid quantum mechanical/molecular mechanical (qm/mm) calculations. the effects of anionic substrate, protein environment, and proximal ligand mutation on the high-valent iron-oxo species, compound i (cpd i), and the ferric hydroperoxide complex, compound 0 (cpd 0), are studied. the results indicate that the presence of an anionic substrate (acetate) and the protonation state of one critical residue (glu104) have a considerab ... | 2009 | 19572690 |
| development of a multichannel flow-through chemiluminescence microarray chip for parallel calibration and detection of pathogenic bacteria. | pathogen detection is important for health and safety reasons. several outbreaks all over the world have shown the need for rapid, qualitative, quantitative, and, particularly, multianalyte detection systems. hence, a multichannel flow-through chemiluminescence microarray chip for parallel detection of pathogenic bacteria was developed. the disposable chip made of acrylonitrile-butadiene-styrene (abs) copolymer was devised as a support for a multiplexed sandwich immunoassay. calibration and meas ... | 2009 | 19575190 |
| screen-printed immunosensor modified with carbon nanotubes in a continuous-flow system for the botrytis cinerea determination in apple tissues. | botrytis cinerea is a plant-pathogenic fungus that produces the disease known as grey mould in a wide variety of agriculturally important hosts in many countries. this paper describes the development of an immunosensor coupled to carbon-based screen-printed electrodes (spce) modified with multi-walled carbon nanotubes (cnts), which show a rapid and sensitive determination of b. cinerea in apple tissues (red-delicious) using a competitive immunoassay method. both the infected plant tissue sample ... | 2009 | 19576430 |
| development of a rapid and sensitive magnetic chemiluminescent enzyme immunoassay for detection of luteinizing hormone in human serum. | a rapid and sensitive magnetic particles (mps)-based chemiluminescence enzyme immunoassay (mps-cleia) for determination of luteinizing hormone (lh) in human serum was developed and validated. | 2009 | 19576875 |
| magnetic particle-based chemiluminescence enzyme immunoassay for free thyroxine in human serum. | a magnetic particles-based chemiluminescence enzyme immunoassay with high sensitivity, specificity, rapidity, and reproducibility was developed for the determination of free thyroxine in human serum. a competitive assay has been proposed with horseradish peroxidase labeled thyroxine analog. the immunomagnetic particles coated with anti-fluorescein isothiocyanate antibody was used as dispersed solid phase and separation means for the immunoassay. experimental conditions, such as temperature, the ... | 2009 | 19581068 |
| analog noise reduction in enzymatic logic gates. | in this work, we demonstrate both experimentally and theoretically that the analog noise generation by a single enzymatic logic gate can be dramatically reduced to yield gate operation with virtually no input noise amplification. we demonstrate that when a cosubstrate with a much smaller affinity than the primary substrate is used, a negligible increase in the noise output from the logic gate is obtained, as compared to the input noise level. our general theoretical conclusions were confirmed by ... | 2009 | 19583193 |
| measurements of heme relaxation and ligand recombination in strong magnetic fields. | heme cooling signals and diatomic ligand recombination kinetics are measured in strong magnetic fields (up to 10 t). we examined diatomic ligand recombination to heme model compounds (no and co), myoglobin (no and o(2)), and horseradish peroxidase (no). no magnetic field induced rate changes in any of the samples were observed within the experimental detection limit. however, in the case of co binding to heme in glycerol and o(2) binding to myoglobin, we observe a small magnetic field dependent ... | 2009 | 19588986 |
| a disposable and cost efficient microfluidic device for the rapid chip-based electrical detection of dna. | requirements for a point-of-care device are an easy and robust read-out and--above all--a simple handling. we integrated an established robust electrical read-out for dna-chips into a microfluidic device, thereby creating an automated analysis system that combines the necessary steps for a chip-based analysis. it is based on the electrical detection of biotin-labeled dna in a gap between two microstructured electrodes on the surface of a dna-chip. the biotin serves as binding molecule for strept ... | 2009 | 19592230 |
| photothermal studies of co photodissociation from peroxidases from horseradish and soybean. | in this work, the results of photoacoustic calorimetry (pac) studies involving co photodissociation from horseradish peroxidase (hrp) and soybean peroxidase (sbp) are discussed. both proteins contain fe-protoporphyrin ix active sites and relatively open distal heme pockets (i.e., direct solvent access). in addition, it has been shown previously that sbp binds a tris molecule in the distal pocket near the heme group potentially regulating ligand binding to the heme iron. results of pac studies in ... | 2009 | 19595798 |
| identification of the neural pathway underlying spontaneous crossed phrenic activity in neonatal rats. | cervical spinal cord hemisection at c2 leads to paralysis of the ipsilateral hemidiaphragm in rats. respiratory function of the paralyzed hemidiaphragm can be restored by activating a latent respiratory motor pathway in adult rats. this pathway is called the crossed phrenic pathway and the restored activity in the paralyzed hemidiaphragm is referred to as crossed phrenic activity. the latent neural pathway is not latent in neonatal rats as shown by the spontaneous expression of crossed phrenic a ... | 2009 | 19596054 |
| transtactin: a universal transmembrane delivery system for strep-tag ii-fused cargos. | the delivery of molecules into cells poses a critical problem that has to be solved for the development of diagnostic tools and therapeutic agents acting on intracellular targets. cargos which by themselves cannot penetrate cellular membranes due to their biophysical properties can achieve cell membrane permeability by fusion to protein transduction domains (ptds). here, we engineered a universal delivery system based on ptd-fused strep-tactin, which we named transtactin. biochemical characteriz ... | 2010 | 19602053 |
| retrograde axonal transport of horseradish peroxidase from spinal cord to brain stem cell groups in the cat. | 1975 | 19604744 | |
| stimulation-induced uptake of horseradish peroxidase by rat cortical synapses. | 1975 | 19604745 | |
| labelling of cells in the medulla oblongata and the spinal cord of the monkey after injections of horseradish peroxidase in the thalamus. | injections of horseradish peroxidase were performed in macaque monkeys in the intralaminar and the ventroposterior thalamic region, respectively. following injection in the intralaminar region labelled cells were present in the marginal zone both in the spinal trigeminal nuclues and in the spinal dorsal horn. the same areas contained labelled cells after injections in the ventroposterior thalamic region, but in such experiments labelled cells were also present in the lamina iv-v region of the sp ... | 1975 | 19604756 |
| the central cervical nucleus - a source of spinocerebellar fibres, demonstrated by retrograde transport of horseradish peroxidase. | injections of horseradish peroxidase in the cerebellum of kittens resulted in labelling of cells in the central cervical nucleus (ccn) in the upper cervical spinal cord. the largest number of labelled ccn neurones was found after injections including the anterior part of the anterior lobe of the cerebellum. in this neuronal system horseradish peroxidase was transported at a maximal velocity of about 150 mm/day. | 1975 | 19604757 |
| morphological correlates of visual field transformation in the corpus callosum. | horseradish peroxidase (hrp) injections at the boundary of areas 17 and 18 in cat visual cortex resulted in retrograde labelling of neurones located in contralateral areas 17 and 18. the hrp-positive neurones were spread over a region located around the representation of the vertical meridian; they were most numerous in the locus for area centralis. layer iii pyramidal cells and stellate cells in upper layer iv were identified as the principal neurones of origin of the visual callosal pathway. | 1976 | 19604766 |
| substantia nigra projections to the reticular formation, superior colliculus and central gray in the rat, cat and monkey. | after horseradish peroxidase injections in different parts of the lower brain stem retrogradely labeled neurons were observed in the substantia nigra, mainly ipsilateral pars reticulata. these findings demonstrate that the substantia nigra projects not only to the striatum and thalamus but also to brain stem areas which give rise to descending spinal projections. these nigral projections to brain stem structures may play an important role in a variety of behaviors and may be involved in clinical ... | 1976 | 19604767 |
| injection of horseradish peroxidase solution in calibrated volumes (nanolitre) into the spinal cord. | a method for the injection of horseradish peroxidase (hrp) into the spinal cord (sc) is described, based on the principle of fluid expansion upon application of heat. hrp solution in the tip of a glass micropipette (tip diameter 10-15 microm) was extruded when the sealed stem of the pipette, containing distilled water, was warmed. the pipette was calibrated (temperature/volume relation) before it was inserted into the sc and the injection of hrp was made according to the calibration curve. using ... | 1976 | 19604813 |
| projections to gyrus sigmoideus from the substantia nigra in the cat, as revealed by the horseradish peroxidase retrograde transport technique. | after injections of horseradish peroxidase in the gyrus sigmoideus of the cat frontal cortex, labelled neurones were found in the substantia nigra and the ventral tegmental area of tsai. neurones distributed bilaterally clearly predominated in the side of the injection in the rostral sections, but differences in number were less at caudal levels. the present findings confirm the existence of a direct mesencephalocortical projection from this ventral tegmental region, and provide a morphological ... | 1976 | 19604816 |
| combined light and electron microscopic tracing of neurons, including axons and synaptic terminals, after intracellular injection of horseradish peroxidase. | by using intracellular deposition of horseradish peroxidase (hrp), a method has been designed for combined light and electron microscopic tracing of single fibers and synaptic terminals, belonging to physiologically characterized neurons. this technique has been successfully tested on the axon collateral systems of cat alpha-motoneurons, but should also be applicable to other types of neurons. | 1976 | 19604850 |
| the absence of horseradish peroxidase uptake by cerebellar afferents to the red nucleus of the cat. | horseradish peroxidase (hrp) administered to the red nucleus (rn) failed to be transported retrogradely in the axons of the cerebellorubral projection system. this negative result was independent of the anaesthesia, fixation method, injection time or survival period. the absence of hrp uptake and transport in our work reflects some uncertainty in the general usefulness of the hrp technique. | 1976 | 19604851 |
| simultaneous demonstration of horseradish peroxidase and acetylcholinesterase. | simultaneous demonstration of acetylcholinesterase (ache) and horseradish peroxidase (hrp) has been achieved by staining frozen sections with a modified koelle-friedenwald thiocholine method for ache followed directly by the graham-karnovsky procedure for hrp. by using sodium sulfite instead of sodium sulfide in the ache procedure, the final reaction product appears as black ache granules that contrast sharply with the yellowish-brown hrp granules. | 1976 | 19604859 |
| simultaneous visualization of chromatolytic neurons and neurons retrogradely labeled with horseradish peroxidase. | a method is described for visualization of neurons retrogradely labeled with horseradish peroxidase (hrp) in celloidin-embedded brain slabs. the preservation of cellular detail obtained with this technique facilitates (1) accurate delimination of nuclei or laminae in which hrp-positive neurons are found and (2) simultaneous identification of cells of origin of two central fiber systems, by combining the retrograde transport of hrp and the retrotrade degeneration technique. the latter approach ha ... | 1976 | 19604881 |
| spinocervical tract cells in cat and dog, labeled by the retrograde transport of horseradish peroxidase. | the location of cells of origin of the spinocervical tract has been determined in cat and dog by injecting horseradish peroxidase into the lateral cervical nucleus (lcn). retrogradely labeled cells were found predominantly in the ipsilateral nucleus proprius of the dorsal horn along the entire spinal cord. cells of clarke's column and contralateral ventral horn cells were not labeled, in agreement with previous electrophysiological indications that the spinocerebellar tracts do not maintain coll ... | 1976 | 19604882 |
| cells of origin of hypothalamo-spinal projections in the rat. | the retrograde transport of horseradish peroxidase (hrp) was used to determine the distribution of hypothalamic neurons which send axonal projections to the spinal cord. labeled cells were observed in the paraventricular nucleus (pv) and in the lateral hypothalamic area following hrp injections into the lower thoracic and upper lumbar spinal cords of rats. in pv, the hrp-labeled cells were located in the parvocellular portion of the nucleus. hypothalamo-spinal projections are predominantly uncro ... | 1976 | 19604883 |
| effects of apomorphine and haloperidol on somatopetal transport of horseradish peroxidase in the nigro-striatal pathway. | labeling of cell bodies in pars compacta of substantia nigra (snc) following injections of horseradish peroxidase (hrp) in the neostriatum was not affected by repeated systemic injections of apomorphine or haloperidol during the survival time. these drugs decelerate and accelerate, respectively, the firing of snc neurons. provided the used standard method was sensitive enough, it could be concluded that at least in these neurons the somatopetal transport and accumulation of the tracer do not dep ... | 1976 | 19604884 |
| retrograde labeling of central nervous pathways with tritiated or evans blue-labeled bovine serum albumin. | in an attempt to develop a technique for the retrograde tracing of neuronal pathways which does not depend on enzyme activity of horseradish peroxidase (hrp), we have examined the retrograde transport of bovine serum albumin (bsa) by neurons of the central nervous system. following injections of either tritiated or evans blue-labeled bsa into the hippocampal formation of the rat, labeled neurons were found in the ipsilateral entorhinal area in a pattern which was highly reminiscent of that obtai ... | 1976 | 19604885 |
| golgi-like staining of neocortical neurons using retrogradely transported horseradish peroxidase. | neocortical pyramidal neurons stained solidly with horseradish peroxidase (hrp) two days after striatal, thalamic or cortical hrp injections are described in the rabbit, rat and marmoset monkey. the dendritic detail seen in these solid hrp-labeled neurons approaches that produced by the golgi method. | 1976 | 19604892 |
| rhombo-mesencephalic connections in the fast conducting electrosensory system of the mormyrid fish, gnathonemus petersii. an hrp study. | using retrograde horseradish peroxidase (hrp) axonal transport direct rhombo-mesencephalic connections were revealed in gnathonemus petersii. by hrp injection in the neuropil of the extrolateral mesencephalic ganglion the neurons of the lateral line lobe nucleus were labeled. the pathway is mainly composed of crossed fibers. | 1976 | 19604893 |