Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| bienzyme functionalized three-layer composite magnetic nanoparticles for electrochemical immunosensors. | the preparation, characterization and application of a three-layer magnetic nanoparticle composed of an fe(3)o(4) magnetic core, a prussian blue (pb) interlayer and a gold shell (it can be abbreviated as au-pb-fe(3)o(4)) for an ultrasensitive and reproducible electrochemical immunosensing fabrication were described for the first time in this work. with the employment of the au-pb-fe(3)o(4) nanoparticle, a new signal amplification strategy was developed based on bienzyme (horseradish peroxidase a ... | 2009 | 19162316 |
| development of an electrochemical immunosensor for aflatoxin m1 in milk with focus on matrix interference. | a simple sensor method was developed for aflatoxin m(1) analysis to be applied directly with milk by using antibody modified screen-printed carbon working electrode with carbon counter and silver-silver chloride pseudo-reference electrode. a competitive elisa assay format was constructed on the surface of the working electrode using 3,3,5',5'-tetramethylbenzidine dihyrochloride (tmb)/h(2)o(2) electrochemical detection scheme with horseradish peroxidase (hrp) as the enzyme label. the performance ... | 2009 | 19167207 |
| high-throughput quantitative analysis of plant n-glycan using a dna sequencer. | high-throughput quantitative analytical method for plant n-glycan has been developed. all steps, including peptide n-glycosidase (pngase) a treatment, glycan preparation, and exoglycosidase digestion, were optimized for high-throughput applications using 96-well format procedures and automatic analysis on a dna sequencer. the glycans of horseradish peroxidase with plant-specific core alpha(1,3)-fucose can be distinguished by the comparison of the glycan profiles obtained via pngase a and f treat ... | 2009 | 19167352 |
| single-crystal ceo2 nanocubes used for the direct electron transfer and electrocatalysis of horseradish peroxidase. | a new horseradish peroxidase (hrp) third-generation electrochemical biosensor based on ceria nanocubes (ceo(2)-ncs) and chitosan (chit) was developed. the single-crystalline, uniform and size-controlled ceo(2)-ncs have been synthesized by hydrothermal method. hrp was immobilized in ceo(2)-ncs and chit film on the glass carbon electrode (hrp/ceo(2)/chit/gce). compared with hrp-chitosan modified electrode (hrp/chit/gce), hrp/ceo(2)/chit/gce exhibited a pair of more obvious redox peaks at -0.348 v ... | 2009 | 19168345 |
| fingerprinting antioxidative activities in plants. | a plethora of concurrent cellular activities is mobilised in the adaptation of plants to adverse environmental conditions. this response can be quantified by physiological experiments or metabolic profiling. the intention of this work is to reduce the number of metabolic processes studied to a minimum of relevant parameters with a maximum yield of information. therefore, we inspected 'summary parameters' characteristic for whole classes of antioxidative metabolites and key enzymes. | 2009 | 19171044 |
| enzyme enhanced quantitative determination of multiple dna targets based on capillary electrophoresis. | in the current paper, enzyme enhanced simultaneous quantitative determination of multiple dna targets based on capillary electrophoresis (ce) was described. we used three biotin-modified dna probes, which reacted with avidin-conjugated horseradish peroxidase (avidin-hrp) conjugate to obtain the hrp labeled probes, to hybridize with three corresponding targets. the resulting mixture containing double-strand dna (dsdna)-hrp, excess single-strand dna (ssdna)-hrp and remaining avidin-hrp was separat ... | 2009 | 19171352 |
| deodorization of swine manure slurry using horseradish peroxidase and peroxides. | considering the development of highly confined piggery and increasing complaints about livestock manure odors, it is pressing to develop a practical way to reduce the odors. peroxidase, which has been proved to be capable of removing toxic phenolic compounds from wastewater, may also be effective in deodorizing the swine manures. horseradish peroxidase (hrp) (0.1-3.0 u/ml) with hydrogen peroxide (h(2)o(2), 0.5-6%) or calcium peroxide (cao(2), 0.1-3.0g) was examined for the efficiency of controll ... | 2009 | 19171425 |
| immunosensing system for alpha-fetoprotein through boronate immunoaffinity column in combination with flow injection chemiluminescence. | a novel immunosensing system for determination of human alpha-fetoprotein (afp) was proposed by using a boronate immunoaffinity column as the glycated antigen collector in combination with flow injection chemiluminescence. the column was fabricated by filling boronic acid-modified sepharose gel into a glass tube. with a sugar-boronic acid interaction, the afp antigen could be effectively immobilized on the sepharose gel matrix. after an off-line incubation, the mixture of the analyte afp and hor ... | 2009 | 19173042 |
| [htert promoter enhances the radiosensitivity to gene-radiation therapy of human laryngeal carcinoma transplanted in nude mice]. | to explore the therapeutic efficiency of human telomerase reverse transcriptase promoter (htertp) mediated horseradish peroxidase (hrp) catalyzed effects of indole-3-acetic (iaa) on laryngeal squamous cell carcinoma with different radiosensitivity in vivo. | 2008 | 19173799 |
| direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in hybrid organic-inorganic film of chitosan/sol-gel/carbon nanotubes. | a hybrid organic-inorganic nanocomposite film of chitosan/sol-gel/multi-walled carbon nanotubes was constructed for the immobilization of horseradish peroxidase (hrp). this film was characterized by scanning electron microscopy. direct electron transfer (det) and bioelectrocatalysis of hrp incorporated into the composite film were investigated. the results indicate that the film can provide a favorable microenvironment for hrp to perform det on the surface of glassy carbon electrodes with a pair ... | 2009 | 19174213 |
| injectable chitosan-based hydrogels for cartilage tissue engineering. | water-soluble chitosan derivatives, chitosan-graft-glycolic acid (ga) and phloretic acid (pa) (ch-ga/pa), were designed to obtain biodegradable injectable chitosan hydrogels through enzymatic crosslinking with horseradish peroxidase (hrp) and h2o2. ch-ga/pa polymers were synthesized by first conjugating glycolic acid (ga) to native chitosan to render the polymer soluble at ph 7.4, and subsequent modification with phloretic acid (pa). the ch-ga43/pa10 with a degree of substitution (ds, defined as ... | 2009 | 19176242 |
| the role of arginine 38 in horseradish peroxidase enzyme revisited: a computational investigation. | molecular dynamics simulations on hydrogen peroxide complex with wild-type (wt) and arg38leu mutated (r38l) horseradish peroxidase (hrp) were carried out over nanoseconds timescale in water solution at 300 k. comparison of the results provides interesting insights about the role of highly conserved arg38 and his42 residues in the chemical features of hrp, underlying its biological activity which initiates with compound0 (cpd0). in the wt-hrp enzyme current molecular dynamics simulations show, fo ... | 2009 | 19178992 |
| dorsal cochlear nucleus projections to the inferior colliculus in the cat: a light and electron microscopic study. | in the present report retrograde and anterograde labeling techniques are used to study the projections of the dorsal cochlear nucleus (dcn) to the inferior colliculus in the cat. horseradish peroxidase (hrp) or wheat germ agglutinin (wga-hrp) injections into the inferior colliculus produce large numbers of labeled neurons in the dcn on the opposite side. labeled cells with projections to the colliculus are identified as fusiform and giant cells and are organized into rostrocaudal bands. the axon ... | 1984 | 19180810 |
| connections between the thalamus and the somatosensory areas of the anterior ectosylvian gyrus in the cat. | the thalamocortical and corticothalamic connections of the second somatic sensory area (sii) and adjacent cortical areas in the cat were studied with anterograde and retrograde tracers. injections consisted of horseradish peroxidase conjugated to wheat germ agglutinin (hrp-wga) or a mixture of equal parts of tritiated leucine and proline. the cortical regions to be injected were electrophysiologically studied with microelectrodes to determine the localization of the selected components of the bo ... | 1984 | 19180811 |
| gut health immunomodulatory and anti-inflammatory functions of gut enzyme digested high protein micro-nutrient dietary supplement-enprocal. | enprocal is a high-protein micro-nutrient rich formulated supplementary food designed to meet the nutritional needs of the frail elderly and be delivered to them in every day foods. we studied the potential of enprocal to improve gut and immune health using simple and robust bioassays for gut cell proliferation, intestinal integrity/permeability, immunomodulatory, anti-inflammatory and anti-oxidative activities. effects of enprocal were compared with whey protein concentrate 80 (wpc), heat treat ... | 2009 | 19183498 |
| paramagnetic 13c and 15n nmr analyses of cyanide- (13c15n-) ligated ferric peroxidases: the push effect, not pull effect, modulates the compound i formation rate. | paramagnetic (13)c and (15)n nmr spectroscopy of heme-bound cyanide ((13)c(15)n) was utilized to quantitatively distinguish the electron donor effect (the push effect) from the proximal histidine and hydrogen-bonding effect (the pull effect) from the distal amino acid residues in cytochrome c peroxidase (ccp), ascorbate peroxidase (apx), lignin peroxidase (lip), and manganese peroxidase (mnp). paramagnetic (13)c nmr signals of heme-bound (13)c(15)n of these peroxidases were observed in a wide ra ... | 2009 | 19187033 |
| synthesis of hepcidin derivatives in order to develop standards for immune adsorption method. | mecn, acetonitrile; ecl, enhanced chemiluminescence; edt, 1,2-ethanedithiole; hepc12-a, rabbit anti-human hepcidin igg, affinity purified; hepc13-a, rabbit anti-mouse/human hepcidin igg, affinity purified; hepc61-p, human hepcidin-25 control/blocking synthetic peptide; hrp, horseradish peroxidase; il-6, interleukin-6; klh, keyhole limpet hemocyanin; leap, liver-expressed antimicrobial peptide; nem, n-ethylmaleimide; nmp, n-methyl-pirrolidone; pbs, phosphate buffered saline; pvdf, polyvinylidene ... | 2009 | 19189268 |
| brainstem projection of the vestibular nerve in the guinea pig: an hrp (horseradish peroxidase) and wga-hrp (wheat germ agglutinin-hrp) study. | we explored the course and termination of primary vestibular afferent fibers within the brainstem of the guinea pig by means of anterograde axonal transport of horseradish peroxidase (hrp) and wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp). primary vestibular afferent fibers distribute within the entire vestibular nuclear complex, with the exception of the dorsal part of the lateral vestibular nucleus. the superior vestibular nucleus is characterized by the concentration of te ... | 1990 | 19189709 |
| development of olivocerebellar fibers in the clawed toad, xenopus laevis: a light and electron microscopical hrp study. | an anterograde tracer study was undertaken to provide a light- and electron microscopical description of climbing fiber development in the clawed toad, xenopus laevis, ranging from premetamorphic stages to the adult state. the inferior olive was unilaterally labeled with horseradish peroxidase and the contralateral climbing fiber morphology investigated. at early stages of development, only undifferentiated fibers were observed in the rostral alar plate. at later stages, these fibers form large ... | 1990 | 19189714 |
| supplementary eye field as defined by intracortical microstimulation: connections in macaques. | in macaques, the frontal eye field and the recently defined supplementary eye field play a role in the production of eye movements. whereas the structure and function of the frontal eye field are well understood, little is known about the supplementary eye field. the goal of this study was to determine the connections of the physiologically defined supplementary eye field. in each case, the location of the supplementary eye field was determined by using intracortical microstimulation, the border ... | 1990 | 19189718 |
| changes of cervical dorsal root ganglia induced by compression injury and decompression procedure: a novel rat model of cervical radiculoneuropathy. | our study aimed to establish a model of compression injury of cervical dorsal root ganglia (drg) in the rat and to investigate the pathological changes following compression injury and decompression procedures. thirty rats were divided into three groups: control group receiving sham surgery, compression group undergoing surgery to place a micro-silica gel on c6 drg, and decompression group with subsequent decompression procedure. the samples harvested from the different groups were examined with ... | 2009 | 19191544 |
| systemic tetracycline delays degradation of three different collagen membranes in rat calvaria. | the aim of this study was to quantitatively evaluate the effect of systemic tetracycline (ttc) on the degradation of three different collagen membranes. | 2009 | 19191795 |
| horseradish peroxidase immobilized on poly(thiophene-2-aminophenol-3-thiopheneacetic acid) film electrode with au nanoparticle-fabrication and evaluation as hydrogen peroxide sensor. | enzyme immobilized electrode was fabricated by two methods. in one of the methods, gold-nanoparticles (au-nps) prepared by gamma-irradiation were loaded into the copolymer film and horseradish peroxidase (hrp) was immobilized into the au-nps loaded copolymer film through physical entrapment. in the other method, the au-nps was prepared by electrochemical reduction of au ions on the surface of poly(th-ap-taa) and hrp was immobilized into the au-nps. the enzyme immobilized electrodes were tested f ... | 2008 | 19198371 |
| cooperative multicomponent self-assembly of nucleic acid structures for the activation of dnazyme cascades: a paradigm for dna sensors and aptasensors. | the activation of a dnazyme cascade by the cooperative self-assembly of multicomponent nucleic acid structures is suggested as a method for the amplified sensing of dna, or the specific substrates of aptamers. according to one configuration, the dna analyte 1 is detected by two tailored nucleic acids 2 and 3 that form a multicomponent supramolecular structure with a ribonucleobase-containing quasi-circular dna 4, but only upon the concomitant hybridization with 1. the resulting supramolecular nu ... | 2009 | 19206117 |
| computer-aided mapping of vasopressin neurons in the hypothalamus of the male golden hamster: evidence of magnocellular neurons that do not project to the neurohypophysis. | abstract vasopressin-sensitive neurons in the region of the anterior hypothalamus are necessary for the mediation of flank marking behavior in the golden hamster. the precise nature of the vasopressinergic innervation to the anterior hypothalamus is unknown. in this study we seek to examine the potential sources of this innervation by mapping and counting the vasopressin-immunoreactive neurons that contribute to the hypothalamo-neurohypophysial system, and those that do not. vasopressin-immunore ... | 1990 | 19210373 |
| detection of known allergen-specific ige antibodies by immunological methods. | an increasing number of patients are suffering from allergic diseases such as rhinoconjunctivitis, atopic eczema, uticaria, anaphylaxis, and food and drug allergies. although it is possible to measure a multitude of allergen-specific ige antibodies by radio or enzyme immunoassays in the patients' blood, these tests are expensive, time-consuming, and usually need a rather high volume of reagent solutions (allergens and blood). protein microarrays offer the possibility to circumvent these limitati ... | 2009 | 19212717 |
| efficient hybridoma screening technique using capture antibody based microarrays. | the hybridoma screening is a key step for the successful generation of high-affinity analyte-specific monoclonal antibodies (mabs), particularly if the target of the antibody is a low-molecular weight analyte. this work presents an advanced screening method that makes use of antibody microarrays generated by contact printing of the hybridoma cell supernatant samples on glass chips initially coated with capture antibodies. the noncompetitive immunoassay is based on the specific binding of an anal ... | 2009 | 19215064 |
| the role of n-methyl-d-aspartate-type glutamatergic neurotransmission in the photic induction of immediate-early gene expression in the suprachiasmatic nuclei of the syrian hamster. | abstract this study investigated the role of n-methyl-d-aspartate (nmda)-type glutamatergic neurotransmission in mediating the photic induction of immediate-early gene expression in the suprachiasmatic nucleus (scn) of the syrian hamster. activation of c-fos, c-jun and egr-1 was assessed by immunocytochemical detection of their protein products. to characterize the circadian basis to the inductive effects of light, hamsters were allowed to free-run in constant dim red light and received a 1 h li ... | 1991 | 19215534 |
| ultrasensitive immunosensor for cancer biomarker proteins using gold nanoparticle film electrodes and multienzyme-particle amplification. | a densely packed gold nanoparticle platform combined with a multiple-enzyme labeled detection antibody-magnetic bead bioconjugate was used as the basis for an ultrasensitive electrochemical immunosensor to detect cancer biomarkers in serum. sensitivity was greatly amplified by synthesizing magnetic bioconjugates particles containing 7500 horseradish peroxidase (hrp) labels along with detection antibodies (ab2) attached to activated carboxyl groups on 1 microm diameter magnetic beads. these senso ... | 2009 | 19216571 |
| fluorescence in situ hybridization for intracellular localization of nifh mrna. | few reports on in situ mrna detection in bacteria have been published, even though a major aim in environmental microbiology is to link function/activity to the identity of the organisms. this study reports a reliable approach for the in situ detection of nifh mrna using fluorescence hybridization based on a previously described protocol for pmoa. nifh codes for a dinitrogenase reductase, a key enzyme in dinitrogen fixation. nifh mrna was hybridized with a digoxigenin-labelled polynucleotide pro ... | 2009 | 19217232 |
| amperometric phenol biosensor based on horseradish peroxidase entrapped pvf and ppy composite film coated gc electrode. | polyvinylferrocene (pvf) was used as a mediator for the fabrication of a horseradish peroxidase (hrp)-modified electrode to detect phenol derivatives via a composite polymeric matrix of conducting polypyrrole (ppy). through an electropolymerization process, enzyme hrp was entrapped with ppy in a three-electrode system onto a glassy carbon electrode previously covered with pvf, resulting in a composite polymeric matrix. steady-state amperometric measurements were performed at -200 mv vs. ag/agcl ... | 2010 | 19224403 |
| low-density macroarray for rapid detection and identification of crimean-congo hemorrhagic fever virus. | crimean-congo hemorrhagic fever (cchf) is a tick-borne viral zoonosis which occurs throughout africa, eastern europe, and asia and results in an approximately 30% fatality rate. a reverse transcription-pcr assay including a competitive internal control was developed on the basis of the most up-to-date genome information. biotinylated amplification products were hybridized to dna macroarrays on the surfaces of polymer supports, and hybridization events were visualized by incubation with a strepta ... | 2009 | 19225100 |
| piezoelectric inkjet printing of a cross-hatch immunoassay on a disposable nylon membrane. | the development of a cost-effective method for manufacturing immunoassays is a key step towards their commercial use. in this study, a piezoelectric inkjet printer and a nylon membrane were used to fabricate a disposable immunoassay. using a piezoelectric inkjet printer, a cross-hatch pattern of goat anti-mouse antibody (galpham) and rabbit anti-horseradish peroxidase (ralphahrp) antibody were deposited on the nylon membrane. these patterns were subsequently treated with a solution containing ra ... | 2009 | 19226553 |
| development of a whole-cell biosensor by cell surface display of a gold-binding polypeptide on the gold surface. | cell surface display was used as a strategy to display the gold-binding polypeptide (gbp) fusion protein on the surface of escherichia coli, and consequently to immobilize the cells on the gold surface. the dna encoding the gbp was fused to the truncated fadl gene and was expressed by the tac promoter. for the display of the core streptavidin (csa) of streptomyces avidinii, the csa gene was fused to the truncated oprf gene. after the dual display of fadl-gbp and oprf-csa on the surface of e. col ... | 2009 | 19228193 |
| immunoperoxidase staining of alveolar hydatid cyst from an experimentally infected gerbil. | echinococcus multilocularis, the small fox tapeworm, has an extensive geographical range in the northern hemisphere where foxes and small rodents represent natural hosts. the larval stage of this parasite, alveolar echinococcosis (ae), is an emerging zoonosis of increasing importance. it is a serious human illness which is often misdiagnosed as hepatic cancer. if not identified at an early stage of parasite development it can lead to the death of patients. histological examination of biopsies is ... | 2003 | 19230579 |
| detection of salmonella typhimurium using an electrochemical immunosensor. | an electrochemical immunosensor based on screen-printed gold working electrode with onboard carbon counter and silver-silver chloride pseudo-reference electrode for salmonella typhimurium detection is described in this paper. monoclonal anti-s. typhimurium antibody was immobilized using physical and covalent immobilization via amine coupling of carboxymethyldextran on the surface of the gold working electrode. a direct sandwich enzyme-linked immunosorbent assays (elisa) format was then developed ... | 2009 | 19233634 |
| [the organization of diencephalic zona incerta projections to the structures of the pallidum in dog brain]. | the detailed organization of the projections of the individual sectors of the zona incerta of the diencephalon to the functionally diverse pallidal structures in dog brain was investigated by the method of antero- and retrograde axonal transport of horseradish peroxidase. it was found that the neurons of the caudal incertal sector innervated the globus pallidus and the nucleus entopeduncularis which predominantly received the innervation from the motor structures. the projections from single neu ... | 2008 | 19241863 |
| [localization of the neurons of the amygdala central nucleus projecting to the hypothalamic paraventricular nucleus area]. | the aim of this study was to identify, using retrograde axonal transport of horseradish peroxidase in rats, the cells, within distinct divisions of the amygdala central nucleus, innervating hypothalamic paraventricular nucleus area. the labeled cells were found in the ipsilateral amygdala central nucleus in the whole of its rostro-caudal extension. the maximal number of labeled neurons was detected in the middle third of the nucleus, corresponding to the intermediate subnucleus of the central nu ... | 2008 | 19241875 |
| superior activity of structurally deprived enzyme-carbon nanotube hybrids in cationic reverse micelles. | in the present work, we report the superior activity of hydrophobically adsorbed enzymes onto single-walled carbon nanotubes (swnts) in the reverse micelles of cationic surfactants. horseradish peroxidase and soybean peroxidase adsorbed onto swnts endure a notable loss in secondary structure and catalytic activity. this structurally and functionally deformed enzyme-swnt when confined in ctab reverse micelles showed approximately 7-9-fold enhancement in activity compared to that was in water and ... | 2009 | 19245221 |
| microsurgical excision of multiple clear cell meningiomas of the cauda equina: a case report. | we report the case of a 21-year-old woman who presented with a 2-year history of worsening radicular pain on the right leg. the valsalva manoeuvre provoked radicular pain and radiography showed right-convex 36 degrees scoliosis. examination showed slight hypoesthesia on the right l3-s1 dermatomes but abnormal muscle power and reflexes. magnetic resonance imaging identified cauda equina tumours at the l2-3 and l4 levels. the tumours showed heterogeneously isointense signals on t(1)-weighted image ... | 2009 | 19247902 |
| in situ quantitative immunoprofiling of regulatory t cells using laser scanning cytometry. | laser scanning cytometry (icys; compucyte, cambridge, mass) has recently been developed to use fluorescence-based quantitative measurements on tissue sections or other cellular preparations at a single-cell level. the purpose of this study was to develop objective, quantitative immunoprofiling of regulatory t cells (t regs) on formalin-fixed/paraffin-embedded (ffpe) biopsy samples from transplanted allografts using icys. we sought to evaluate the usefulness of icys to analyzes the population of ... | 2009 | 19249524 |
| electrochemical immunosensor for the diagnosis of celiac disease. | a novel electrochemical immunosensing strategy for the detection of antibodies to tissue transglutaminase (ttg) in human serum is presented. the proposed immunosensor consists of the immobilization by physical adsorption of ttg from guinea pig liver on graphite-epoxy composite (gec) electrodes. after the reaction with the human serum (containing the specific antibodies in the case of celiac disease), the electrode was incubated with different kinds of secondary labeled antibodies, namely, horser ... | 2009 | 19250919 |
| enzyme-containing hydrogel micropatterns serving a dual purpose of cell sequestration and metabolite detection. | the integration of sensing elements with small groups of cells is a critical step towards miniaturization of cell cultivation and analysis. this paper describes the development of an optical, enzyme-based biosensor for local detection of hydrogen peroxide (h(2)o(2)) secreted by stimulated macrophages. photolithographic patterning of horseradish peroxidase (hrp)-containing poly (ethylene glycol) (peg) hydrogel microstructures was used to create sensing structures on the glass surface. importantly ... | 2009 | 19251408 |
| rapid mycobacterial liquid culture-screening method for mycobacterium avium complex based on secreted antigen-capture enzyme-linked immunosorbent assay. | sensors in automated liquid culture systems for mycobacteria, such as mgit, bact/alert 3d, and trek esp ii, flag growth of any type of bacteria; a positive signal does not mean that the target mycobacteria are present. all signal-positive cultures thus require additional and often laborious testing. an immunoassay was developed to screen liquid mycobacterial cultures for evidence of mycobacterium avium complex (mac). the method, called the mac-enzyme-linked immunosorbent assay (elisa), relies on ... | 2009 | 19261776 |
| multiplex chemiluminescence microscope imaging of p16(ink4a) and hpv dna as biomarker of cervical neoplasia. | classification of cervical intraepithelial neoplasia (cin) lesions in low-grade (cin1) or high-grade (cin2-3) ones is crucial for optimal patient management, but current histological diagnosis on bioptic samples is often hampered by inter-observer variability. to allow objective classification, we have exploited the peculiar characteristics of chemiluminescence detection, such as high sensitivity and easy quantification of the luminescence signal, to perform sequentially in the same tissue secti ... | 2009 | 19263042 |
| detection of proteinase k resistant proteins in the urine of patients with creutzfeldt-jakob and other neurodegenerative diseases. | recent concern about the possible secondary spread of vcjd through blood transfusion and blood products has highlighted the need for a sensitive test for the identification of prp(tse/res) in clinical specimens collected in a non-invasive way. in addition, a more accurate estimate of the prevalence of pre-clinical vcjd in the population may be possible if there were a test that could be applied to easily available material such as urine. as a step towards this goal,the detection of putative prp( ... | 2008 | 19263593 |
| reconstructed bladder innervation below the level of spinal cord injury: the knee-tendon to bladder artificial reflex arc. | to study the effectiveness of knee-tendon to bladder artificial reflex arc in dogs. | 2009 | 19264053 |
| in situ real-time chemiluminescence imaging of reactive oxygen species formation from cardiomyocytes. | we have applied the highly sensitive chemiluminescence (cl) imaging technique to investigate the in situ ros formation in cultured monolayers of rat h9c2 cardiomyocytes. photon emission was detected via an innovative imaging system after incubation of h9c2 cells in culture with luminol and horseradish peroxidase (hrp), suggesting constitutive formation of ros by the cardiomyocytes. addition of benzo(a)pyrene-1,6-quinone (bpq) to cultured h9c2 cells resulted in a 4-5-fold increase in the formatio ... | 2009 | 19266051 |
| nanostructured fes as a mimic peroxidase for biocatalysis and biosensing. | artificial enzyme mimics have attracted considerable interest due to easy denaturation and leakage of enzymes during their storage and immobilization procedure. herein we describe the design of a novel mimic peroxidase, a nanostructure of sheet-like fes prepared by a simple micelle-assisted synthetic method. such a nanostructure has a large specific surface area and high peroxidase-like activity, and was thus further used as a mimic enzyme for the development of biocatalysts and amperometric bio ... | 2009 | 19267381 |
| adsorption chromatography of proteins. | a method for adsorption chromatography of proteins is proposed. a protein solution is passed through a cellulose column at a ph value corresponding to an isoelectric point of the protein. depending on the charge of unwanted proteins, they either remain at the origin (if charges of protein and ion-exchanger are opposite) or are released from the column (if charges of protein and ion-exchanger coincide). elution volume of the purified protein is higher than for the second group of unwanted protein ... | 2009 | 19267671 |
| one-step construction of reagentless biosensor based on chitosan-carbon nanotubes-nile blue-horseradish peroxidase biocomposite formed by electrodeposition. | a simple and controllable electrodeposition approach was established for one-step construction of novel reagentless biosensors by in situ formation of chitosan-carbon nanotubes-nile blue-horseradish peroxidase (cs-cnts-nb-hrp) biocomposite film on electrode surface. the mediator effect of nb, conducting performance of cnts and the biocompatible microenvironment of cs were combined by such one-step non-manual process. nb could interact with cnts and resulted in good dispersion of cnts-nb nanocomp ... | 2009 | 19269475 |
| an electrochemical biosensor for alpha-fetoprotein based on carbon paste electrode constructed of room temperature ionic liquid and gold nanoparticles. | a novel and effective electrochemical immunosensor for the rapid determination of alpha-fetoprotein (afp) based on carbon paste electrode (cpe) consisting of room temperature ionic liquid (rtil) n-butylpyridinium hexafluorophosphate (bppf(6)) and graphite. the surface of the cpe was modified with gold nanoparticles for the immobilization of the alpha-fetoprotein antibody (anti-afp). by sandwiching the antigen between anti-afp on the cpe modified with gold nanoparticles and the secondary antibody ... | 2009 | 19269485 |
| characterization of the glutathione conjugate of the semisynthetic flavonoid monoher. | flavonoids protect against oxidative stress by scavenging free radicals. during this protection flavonoids are oxidized. the oxidized flavonoids formed are often reactive. consequently, protection by flavonoids can result in the formation of toxic products. in this study the oxidation of 7-mono-o-(beta-hydroxyethyl)rutoside (monoher), which is a constituent of the registered drug venoruton, was studied in the absence and presence of glutathione (gsh). monoher was oxidized by horseradish peroxida ... | 2009 | 19272444 |
| critical roles of decompression in functional recovery of ex vivo spinal cord white matter. | the correlations between functional deficits, the magnitude of compression, and the role of sustained compression during traumatic spinal cord injury remain largely unknown. thus, the functional outcome of this type of injury with or without surgical intervention is rather unpredictable. to elucidate how severity and duration of compression affect cord function, the authors have developed a method to study electrophysiological characteristics and axonal membrane damage in white matter from guine ... | 2009 | 19278332 |
| the precerebellar linear nucleus in the mouse defined by connections, immunohistochemistry, and gene expression. | the linear nucleus (li) is a prominent cell group in the caudal hindbrain, which was first described in a study of cerebellar afferents in the rat by [watson, c.r.r., switzer, r.c. iii, 1978. trigeminal projections to cerebellar tactile areas in the rat origin mainly from n. interpolaris and n. principalis. neurosci. lett. 10, 77-82.]. it was named for its elongated appearance in transverse sections. since this original description in the rat, reference to the nucleus seems to have been largely ... | 2009 | 19281800 |
| labeling of biotin antibodies with horseradish peroxidase using cyanuric chloride. | in this report, we describe a two-step protocol for labeling of an affinity-purified antibody to biotin with horseradish peroxidase (hrp) using cyanuric chloride (cc) as a bridge. the enzyme was first modified with cc, and following chromatography on a pd-10 column, the activated hrp was incubated with the antibody to effect coupling of the two proteins. assessment of the conjugate product was carried out using elisa and sds-page electrophoresis where evidence for high antibody activity, high sp ... | 2009 | 19282850 |
| recombinant ciliary neurotrophic factor promotes nerve regeneration and induces gene expression in silicon tube-bridged transected sciatic nerves in adult rats. | sciatic nerves in adult male rats were transected and reunited via a silicone chamber. this was followed by a focal injection of recombinant ciliary neurotrophic factor (cntf). to evaluate the effect of this therapeutic approach and to explore its possible mechanisms, nerve regeneration was traced by horseradish peroxidase retrograde labeling. functional recovery was evaluated by functional assessment of the hind feet and the expression of a number of proteins was detected using immunohistochemi ... | 2009 | 19289286 |
| hybrid gravitational field-flow fractionation using immunofunctionalized walls for integrated bioanalytical devices. | in this work, the biospecific recognition antigen-antibody reaction was implemented in gravitational field-flow fractionation (grfff), a flow-assisted separation technique for micron-sized particles, in order to realize a hybrid immunomodulated grfff system in which two different principles are combined to achieve highly versatile fractionation. micron-sized polystyrene beads coated with horseradish peroxidase (hrp) were used as a model sample, and anti-hrp antibodies were immobilized on the acc ... | 2009 | 19290513 |
| catalytic resonance scattering spectral determination of ultratrace horseradish peroxidase using rhodamine s. | a highly sensitive and selective resonance scattering spectral assay was proposed for the determination of horseradish peroxidase (hrp), based on its catalytic effect on the h2o2 oxidation of ki to form i3(-). the i3(-) combined respectively with rhodamine (rh) dye such as rhodamine s (rhs), rhodamine 6g (rh6g), rhodamine b (rhb) and butyl-rhodamine b (b-rhb), to form association particles (rh-i3(n). the four rh systems all exhibit a stronger resonance scattering (rs) peak at 424 nm. for the rhs ... | 2013 | 19291809 |
| expression of trpm8 in the distal cerebrospinal fluid-contacting neurons in the brain mesencephalon of rats. | abstract: | 2009 | 19292918 |
| adrenergic and noradrenergic innervation of the midbrain ventral tegmental area and retrorubral field: prominent inputs from medullary homeostatic centers. | adrenergic agents modulate the activity of midbrain ventral tegmental area (vta) neurons. however, the sources of noradrenergic and adrenergic inputs are not well characterized. immunostaining for dopamine beta-hydroxylase revealed fibers within dopamine (da) neuron areas, with the highest density in the retrorubral field (a8 cell group), followed by the vta (a10 cell group), and very few fibers within substantia nigra compacta. a less dense, but a similar pattern of fibers was also found for th ... | 2009 | 19295165 |
| tumor apoptosis by indole-3-acetic acid/light in b16f10 melanoma-implanted nude mice. | recently, we reported that uvb-activated indole-3-acetic acid (iaa) induces the apoptosis of g361 human melanoma cells. in the present study, we used iaa and visible light combinations to treat b16f10 melanoma-implanted nude mice using an experimental intense pulsed light (ipl) therapy model. we first investigated whether activated iaa by horseradish peroxidase (hrp) or uvb causes apoptosis of b16f10 melanoma cells. iaa/hrp or iaa/uvb combination lead to apoptosis of b16f10 cells, as reported in ... | 2009 | 19301020 |
| quadruple-allele chemiluminometric assay for simultaneous genotyping of two single-nucleotide polymorphisms. | we developed a rapid, simple, cost-effective and high sample-throughput method for the simultaneous detection of four alleles in single-nucleotide polymorphisms (snps). the method was applied to the simultaneous genotyping of two common snps within the tlr4 gene, the a896g and c1196t polymorphisms. the method consists of a single pcr of the region spanning the a896g and c1196t polymorphic sites, followed by a quadruple primer extension (pext) reaction in a single tube. a biotinylated nucleotide ... | 2009 | 19305922 |
| magnetic bead-based dna hybridization assay with chemiluminescence and chemiluminescent imaging detection. | simple and sensitive chemiluminescence (cl) and cl imaging methods have been developed for the magnetic bead-based dna hybridization assay. the assay relies on the high sensitivity and long stable light signal of the cl system in which horseradish peroxidase (hrp) catalyzes the luminol-h(2)o(2) reaction with para-iodophenol (pip) as the enhancer. in this protocol, a sandwich dna hybridization is performed by mixing the target dna with the magnetic bead-captured dna and the biotinylated reporter ... | 2009 | 19305933 |
| enzyme-enhanced fluorescence detection of dna on etched optical fibers. | a novel dna biosensor based on enzyme-enhanced fluorescence detection on etched optical fibers was developed. the hybridization complex of dna probe and biotinylated target was formed on the etched optical fiber, and was then bound with streptavidin labeled horseradish peroxidase (streptavidin-hrp). the target dna was quantified through the fluorescent detection of bi-p,p'-4-hydroxyphenylacetic acid (dbda) generated from the substrate 4-hydroxyphenylacetic acid (p-hpa) under the catalysis of hrp ... | 2009 | 19307112 |
| supramolecular cocaine-aptamer complexes activate biocatalytic cascades. | the anti-cocaine aptamer was fragmented into two nucleic acids, (1) and (2). the nucleic acid (1) was tethered at its 5'-end to aminoethyl nicotinamide adenine dinucleotide, amino-nad(+), or to horseradish peroxidase, hrp. the nucleic acid (2) was functionalized at its 3'-end with alcohol dehydrogenase, alcdh, or with glucose oxidase, gox. in the presence of cocaine, the supramolecular nad(+)/alcdh/cocaine-aptamer complex is formed, and the biocatalytic oxidation of ethanol is activated. similar ... | 2009 | 19309141 |
| an extraction-free method by which a single slot blot can be used to quantify intracellular dna damage (crosslinks or strand breaks) and changes in dna damage response proteins or replication. | we report an extraction-free assay in which the same slot blot membrane can be used to assess total genomic dna damage (i.e., crosslinks or strand breaks) and dna replication (i.e., bromodeoxyuridine incorporation) or protein levels (i.e., gamma-h2ax). 14c-thymidine radiolabeling of hct116 cells loaded directly on a hybond n+ membrane slot blot enables the quantitation of dna interstrand crosslinks and dna breaks, while bromodeoxyuridine incorporation or levels of gamma-h2ax can be assessed by i ... | 2009 | 19317657 |
| columnar organization of estrogen receptor-alpha immunoreactive neurons in the periaqueductal gray projecting to the nucleus para-retroambiguus in the caudal brainstem of the female golden hamster. | in the hamster brainstem estrogen receptor-alpha-immunoreactive neurons (er-alpha-ir) are present in the nucleus para-retroambiguus (npra), located in the caudal ventrolateral medulla (cvlm) ventrolaterally to the nucleus retroambiguus (nra). npra neurons project mainly to the thoracic and upper lumbar cord and are probably involved in the autonomic adaptations during the estrous cycle. the periaqueductal gray (pag), projecting to the cvlm, also contains er-alpha-ir neurons. this raises the ques ... | 2009 | 19321152 |
| stabilization of enzymes in silk films. | material systems are needed that promote stabilization of entrained molecules, such as enzymes or therapeutic proteins, without destroying their activity. we demonstrate that the unique structure of silk fibroin protein, when assembled into the solid state, establishes an environment that is conducive to the stabilization of entrained proteins. enzymes (glucose oxidase, lipase, and horseradish peroxidase) entrapped in these films over 10 months retained significant activity, even when stored at ... | 2009 | 19323497 |
| three-minute-long chemiluminescent immunoassay using dually accelerated immunoreaction by infrared heating and passive mixing. | a rapid chemiluminescent (cl) immunoassay method was developed by integrating a newly designed infrared-radiation technique with a pressure-driven fluidic system. the fluidic system combined a three-dimensional helical glass tube for rapid mixing of immunoreagents with two spiral glass tubes for magnetic separation and cl detection, respectively. the mixture passively formed in the helical glass tube could be quickly heated and kept at about 37 degrees c by the infrared radiation. the immunoreac ... | 2009 | 19323531 |
| self-assembly of enzymes on dna scaffolds: en route to biocatalytic cascades and the synthesis of metallic nanowires. | dna strands consisting of programmed sequence-specific domains were synthesized by the rolling circle amplification (rca) process. the spatial positioning of glucose oxidase (gox) and of horseradish peroxidase (hrp) on the rca-synthesized dna template via hybridization enabled the activation of the bienzyme cascade. the gox-catalyzed oxidation of glucose yielded gluconic acid and h(2)o(2), and the resulting h(2)o(2) oxidized 2,2'-azino-bis[3-ethylbenzthiazoline-6-sulfonic-acid] (abts(2-)) in the ... | 2009 | 19323557 |
| injectable and in situ gelling hydrogels for modified protein release. | a novel injectable polysaccharide system based on calcium alginate (ca-alg) hydrogel and two dextran methacrylate derivatives (dexma) was recently developed. the resulting interpenetrating polymer network showed a synergistic mechanical behavior that can be exploited to target the hydrogel properties towards specific biomedical needs. in the present paper, hydrogels composed of 3% (w/v) ca-alg and dextran (mw 40 x 10(4) and 500 x 10(4)), derivatized with methacrylic groups (derivatization degree ... | 2010 | 19326113 |
| addressable microfluidic polymer chip for dna-directed immobilization of oligonucleotide-tagged compounds. | a microfluidic polymer chip for the self-assembly of dna conjugates through dna-directed immobilization is developed. the chip is fabricated from two parts, one of which contains a microfluidic channel produced from poly(dimethylsiloxane) (pdms) by replica-casting technique using a mold prepared by photolithographic techniques. the microfluidic part is sealed by covalent bonding with a chemically activated glass slide containing a dna oligonucleotide microarray. the dimension of the pdms-glass m ... | 2009 | 19326353 |
| the enzyme-linked immunosorbent assay (elisa) method for nicotine metabolites determination in biological fluids. | the aim of this study was to present the usefulness of enzyme-linked immunosorbent assay (elisa) in nicotine metabolites determination in urine and serum samples from active smokers and compare it with the reference to thin layer chromatography (tlc) with densitometry. the specific anti-cotinine antibodies were obtained from rabbit sera after sequential immunization with 4'-carboxycotinine-hemocyanine conjugate. the immunoaffinity chromatography technique with the use of self-prepared cotinine-a ... | 2009 | 19327926 |
| effect of low and very low doses of simple phenolics on plant peroxidase activity. | changes in the activity of horseradish peroxidase resulting from an addition of ethanol water dilutions of 19 phenolic compounds were observed. for each compound, the enzyme activity was plotted against the degree of dilution expressed as n = -log(100) (mol/l) in the range 0 </= n >/= 20. all the curves showed sinusoidal activity, more or less regular, with two to four peaks on average. each analyzed compound had a characteristic sinusoidal shape, which was constant for samples of peroxidase fro ... | 2004 | 19330128 |
| development of an indirect enzyme immunoassay using monoclonal antibodies for the measurement of 17alpha-hydroxyprogesterone in human serum. | an indirect enzyme immunoassay for the measurement of total 17alpha-hydroxyprogesterone (17ohp) in serum using monoclonal antibodies generated in our laboratory was developed. here, (a) instead of extraction with solvents, serum was heated to free protein-bound 17ohp and assay was performed at ph 9.6, (b) to ensure uniform assay conditions for both standards and samples, buffer for standards contained charcoal-stripped pre-heated pooled cord serum. assays were done in 96-well eia microplates pre ... | 2009 | 19330642 |
| development of elisa for measurement of progesterone employing 17-alpha-oh-p-hrp as enzyme label. | the present study was aimed to develop a highly specific and sensitive enzyme linked immunosorbent assay (elisa) to measure progesterone in human serum using a heterologous combination of immunogen and enzyme conjugate. the antiserum was raised against progesterone-3-o-carboxymethyloxime bovine serum albumin (p-3-o-cmo-bsa) in new zealand white rabbits. the enzyme conjugate was prepared by labeling 17-alpha-hydroxy-progesterone-3-o-carboxymethyloxime (17-alpha-oh-p-3-o-cmo) with horseradish pero ... | 2009 | 19330644 |
| development of an immunoassay to measure progesterone using printed biosensors, and its application to the assessment of ovarian function in the numbat (myrmecobius fasciatus). | a biosensor system was developed to measure progesterone levels in the urine of female numbats (myrmecobius fasciata) as an index of ovarian function. screen printed sensors were coated with a monoclonal progesterone antibody, and incubated in a mixture of sample/standard and progesterone-3-cmo-horseradish peroxidase (hrp). the difference in potential between the working and reference electrode was measured, after exposure to an hrp substrate. eia and biosensor standard curves showed parallelism ... | 2009 | 19330648 |
| surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay. | the activation of a lipopolysaccharide (lps) with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (cdap) in the presence of a surfactant allows an efficient conjugation with dansyl hydrazine or horseradish peroxidase (hrp) in an aqueous medium maintaining its biological activity. in order to promote the reaction a series of amphiphilic compounds were tested, sodium deoxycholate being the most suitable. the method presents several advantages: it is carried out in a mild environment, good conj ... | 2009 | 19334704 |
| electrochemical detection of glyphosate herbicide using horseradish peroxidase immobilized on sulfonated polymer matrix. | this paper describes the use of horseradish peroxidase (hrp) based biosensor for novel detection of glyphosate herbicide. the biosensor was prepared by electrochemically depositing poly(2,5-dimethoxyaniline) (pdma) doped with poly(4-styrenesulfonic acid) (pss) onto the surface of a gold electrode followed by electrostatic attachment of the enzyme hrp onto the pdma-pss composite film. fourier transform infrared (ftir) and uv-vis spectrometry inferred that hrp was not denatured during its immobili ... | 2009 | 19336272 |
| mechanistic aspects of horseradish peroxidase elucidated through single-molecule studies. | many individual horseradish peroxidase (hrp) molecules were isolated and observed simultaneously by fluorescence microscopy in an array of 50 000 femtoliter chambers chemically etched into the surface of a glass optical fiber bundle. the substrate turnovers of hundreds of individual hrp molecules were readily analyzed, and the large number of molecules observed provided excellent statistics. in contrast to other enzymes used for single-molecule studies, the rates of product formation in the femt ... | 2009 | 19338338 |
| nano-elisa for highly sensitive protein detection. | highly sensitive protein detection method based on nanoparticles and enzyme-linked immunosorbent assays (elisas), named nano-elisa, was introduced. in this method, the micro-magnetic beads were modified with monoclonal antibody of the target protein p53. gold nanoparticles (aunps) were modified with another monoclonal detector antibody and horseradish peroxidase (hrp, for signal amplification). the presence of target protein p53 causes the formation of the sandwich structures (magnetic beads-tar ... | 2009 | 19339168 |
| morphological characterization of neurons projecting to the ring gland in the larval blow fly, protophormia terraenovae. | the cephalic neuroendocrine system of the larval blow fly, protophormia terraenovae was studied by backfills using either horseradish peroxidase or nicl2, and peptide immunocytochemistry. backfills through a proximal part of the ring gland mainly revealed three groups of neurons: (1) neurons with somata in the pars intercerebralis (pi) of the protocerebrum, (2) neurons with somata in the pars lateralis (pl) of the protocerebrum, and (3) neurons with somata in the subesophageal ganglion (seg). de ... | 2009 | 19341345 |
| oxidation of melatonin by aaph-derived peroxyl radicals: evidence of a pro-oxidant effect of melatonin. | melatonin is well-established as a powerful reducing agent of oxidant generated in the cell medium. we aimed to investigate how readily melatonin is oxidized by peroxyl radicals roo generated by the thermolysis of 2,2'-azobis(2-amidinopropane) hydrochloride (aaph) and the role of glutathione (gsh) during the reaction course. | 2009 | 19344745 |
| multilayers enzyme-coated carbon nanotubes as biolabel for ultrasensitive chemiluminescence immunoassay of cancer biomarker. | a novel and ultrasensitive chemiluminescence immunoassay (clia) method based on multiple enzyme layers assembled multiwall carbon nanotubes (mwcnts) as signal amplification labels was developed by employing luminol-h(2)o(2)-hrp-bromophenol blue (bpb) enhanced chemiluminescence (cl) system for the detection of a cancer biomarker in human serum samples, as exemplified by the measurement of alpha-fetoprotein (afp) as a model protein. in this study, horseradish peroxidase (hrp) was assembled onto mw ... | 2009 | 19345084 |
| aqp4 gene deletion in mice does not alter blood-brain barrier integrity or brain morphology. | the glial cell water channel aquaporin-4 (aqp4) plays an important role in brain edema, astrocyte migration, and neuronal excitability. zhou et al. [zhou j, kong h, hua x, xiao m, ding j, hu g (2008) altered blood-brain barrier integrity in adult aquaporin-4 knockout mice. neuroreport 19:1-5] recently reported that aqp4 deletion significantly altered blood-brain barrier integrity and glial fibrillary acidic protein (gfap) immunoreactivity in their aqp4 null mice. here we describe a detailed char ... | 2009 | 19345723 |
| is the positive c-kit immunostaining associated with the presence of cells analogous to the intersticial cells of cajal in the ciliary muscle? | interstitial cells of cajal were identified in the gastrointestinal tract of several species, with close relation to the enteric nervous system. since it was recognized that interstitial cells of cajal express the gene product of c-kit, we performed immunohistochemistry for c-kit protein in ciliary muscle specimens of monkeys' eyes. | 2009 | 19347121 |
| chapter 26 measurement of superoxide formation by mitochondrial complex i of yarrowia lipolytica. | complex i (nadh: ubiquinone oxidoreductase) is generally regarded as one of the major sources of mitochondrial reactive oxygen species (ros). mitochondrial membranes from the obligate aerobic yeast yarrowia lipolytica, as well as the purified and reconstituted enzyme, can be used to measure complex i-dependent generation of superoxide (o(2)(*-)). the use of isolated complex i excludes interference with other respiratory chain complexes and matrix enzymes during superoxide dismutase-sensitive red ... | 2009 | 19348905 |
| the chemoenzymatic synthesis of usnic acid. | usnic acid, a highly functionalized dibenzofuran, is a polyketide secondary metabolite produced by several species of lichens. synthesis of usnic acid from commercially available starting material was accomplished in two steps. the synthesis involves the methylation of phloracetophenone followed by oxidation with horseradish peroxidase. this work will lay the foundation for further biosynthetic studies on usnic acid. | 2009 | 19349177 |
| enhanced detection of h2o2 in cells expressing horseradish peroxidase. | a new procedure for fluorescent detection of intracellular h2o2 in cells transiently expressing the catalyst horseradish peroxidase (hrp) is setup and validated. more specific reaction with hrp largely amplifies oxidation of the redox probes used (2',7'-dichlorodihydrofluorescein and dihydrorhodamine). expression of hrp does not affect cell viability. the procedure reveals mao activity, a primary intracellular h2o2 source, in monolayers of intact transfected cells. the probes oxidation rate resp ... | 2009 | 19353363 |
| cytotoxic effects of different concentrations of a carbamide peroxide bleaching gel on odontoblast-like cells mdpc-23. | this study evaluated the cytotoxic effects of a carbamide peroxide (cp) bleaching gel at different concentrations on odontoblast-like cells. immortalized cells of the mdpc-23 cell line (30,000 cells/cm(2)) were incubated for 48 h. the bleaching gel was diluted in dmem culture medium originating extracts with different cp concentrations. the amount (microg/ml) of hydrogen peroxide (h(2)o(2)) released from each extract was measured by the leukocrystal violet/horseradish peroxidase enzyme assay. fi ... | 2009 | 19353567 |
| simple approach for efficient encapsulation of enzyme in silica matrix with retained bioactivity. | we developed an alcohol-free sol-gel approach to encapsulate biomolecules such as horseradish peroxidase (hrp) in an electrochemically induced three-dimensional porous silica matrix by a one-step process. in this sol-gel process, the electrochemically generated hydroxyl ions at the electrode surface by applying cathodic current promote the hydrolysis of ammonium fluorosilicate to produce silica, and simultaneously the generated hydrogen bubbles play an important role in forming porous silica mat ... | 2009 | 19354263 |
| competitive elisa using a rabies glycoprotein-transformed cell line to semi-quantify rabies neutralizing-related antibodies in dogs. | dogs are the principal vectors for rabies virus transmission to humans in many parts of the world. the current "gold standard" world health organization- and office international des epizooties-recommended tests for measuring anti-rabies virus antibodies in dogs, such as the fluorescent antibody virus neutralization (favn) test, requires high containment facilities and several days for results. here, we describe a new competitive elisa (c-elisa) that utilizes a cell line stably expressing the ra ... | 2009 | 19356613 |
| layer by layer immobilized horseradish peroxidase on zinc oxide nanorods for biosensing. | using zinc powders as source material, zno nanorods (znonr) were fabricated on gold wire by a hydrothermal reaction without any other surfactant. the gold wire end was coated by a thin layer of zn-au alloy to improve the nucleation for growth of zno nanostructures and to further improve the performance of the biosensor, which was constructed by alternatively immobilizing poly(sodium 4-styrenesulfonate) (pss) and horseradish peroxidase (hrp) on the znonr. electrochemical measurement, ultraviolet- ... | 2009 | 19358542 |
| efficiency of a bienzyme sequential reaction system immobilized on polyelectrolyte multilayer-coated colloids. | we assembled multilayer films of glucose oxidase (gox) and horseradish peroxidase (hrp) coimmobilized together with polyelectrolyte layers on the surface of silica microparticles. the influence of different polyelectrolyte combinations on the immobilization and functionality of the enzymes was examined for several multilayer configurations. precomplexation of the enzymes with a polyvinylpyridine-based polyamine allowed the stable adsorption of enzyme layers without affecting their catalytic acti ... | 2008 | 19360959 |
| new insights into the detection of sulfur trioxide anion radical by spin trapping: radical trapping versus nucleophilic addition. | it has recently been demonstrated that (bi)sulfite (hydrated sulfur dioxide) reacts with 5,5-dimethyl-1-pyrroline n-oxide (dmpo) via a nonradical, nucleophilic reaction, and further proposed that the radical adduct (dmpo/()so(3)(-)) formation in biological systems is an artifact and not the result of spin trapping of sulfur trioxide anion radical (()so(3)(-)). here, the one-electron oxidation of (bi)sulfite catalyzed by horseradish peroxidase/h(2)o(2) has been reinvestigated by esr spin trapping ... | 2009 | 19362142 |
| investigation of voltammetric enzyme-linked immunoassay system based on n-heterocyclic substrate of 2,3-diaminopyridine. | a new voltammetric enzyme-linked immunoassay system using the electrochemical substrate 2,3-diaminopyridine (dap) and horseradish peroxidase (hrp) system has been developed. dap is oxidized with h2o2 catalyzed by hrp, and the resulting electroactive product produces a sensitive voltammetric peak at potential of -0.72v (vs. saturated calomel electrode (sce)) in britton-robinson (br) buffer solutions. the enzyme-catalyzed reaction conditions and voltammetric detection conditions have been investig ... | 2009 | 19362207 |
| design and synthesis of a photocleavable biotin-linker for the photoisolation of ligand-receptor complexes based on the photolysis of 8-quinolinyl sulfonates in aqueous solution. | the ability of avidin (avn) to form strong complex with biotin (btn) is frequently used in the detection and isolation of biomolecules in biochemical, analytical, and medicinal research. the fact that the binding is nealy irreversible, however, constitutes a drawback in term of the isolation and purification of intact biomolecules. we recently found that 8-quinolinyl esters of aromatic or aliphatic sulfonic acids undergo photolysis when irradiated at 300-330 nm in aqueous solution at neutral ph. ... | 2009 | 19362845 |
| ambivalent effects of diazoxide on mitochondrial ros production at respiratory chain complexes i and iii. | reactive oxygen species (ros) are among the main determinants of cellular damage during ischemia and reperfusion. there is also ample evidence that mitochondrial ros production is involved in signaling during ischemic and pharmacological preconditioning. in a previous study we analyzed the mitochondrial effects of the efficient preconditioning drug diazoxide and found that it increased the mitochondrial oxidation of the ros-sensitive fluorescent dye 2',7'-dichlorodihydrofluorescein (h(2)dcf) but ... | 2009 | 19364480 |
| prostaglandin h synthase-1-catalyzed bioactivation of neurotransmitters, their precursors, and metabolites: oxidative dna damage and electron spin resonance spectroscopy studies. | the role of prostaglandin h synthase-1 (phs-1) and a related model enzyme, horseradish peroxidase (hrp), in catalyzing the bioactivation of dopamine (da) and epinephrine and their precursors and metabolites to potential neurodegenerative free radical intermediates was examined. to determine the potential contribution of phs-dependent reactive oxygen species (ros) formation, the neurotransmitter da or its precursor and metabolites were incubated in vitro with purified ovine phs-1 and calf thymus ... | 2009 | 19374330 |