Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| microtiter enzyme-linked immunosorbent assay for immunoglobulin g cholera antitoxin in humans: method and correlation with rabbit skin vascular permeability factor technique. | a microtiter enzyme-linked immunosorbent assay (elisa) to measure immunoglobulin g cholera antitoxin in human serum has been developed. the elisa employs commercially available reagents, including cholera enterotoxin and goat anti-human immunoglobulin g. it is specific, sensitive, and reproducible and requires as little as 5 microliter of serum. elisa, moreover, permits quantitative determination of cholera antitoxin at a single serum dilution of 1:200. a total of 162 pre- and post-challenge ser ... | 1980 | 7189746 |
| correlation between plasminogen activator activity of immunizing tumor cells and complement-mediated cytotoxic antibodies secreted by cloned hybrid cells. | the present studies examined the biochemical characteristics which were carried on from parent cells during fusion of human skin fibroblasts (hsf) with spleen cells of balb/c mice preimmunized with hormone-responsive and nonresponsive human malignant melanoma cells (hmmc-sha and hmmc-sr). the melanoma cells used as immunogens were either unmodified or preincubated with vibrio cholera neuraminidase (vcn), with estradiol (e), or with progesterone (p). responsiveness was monitored by (3h) thymidine ... | 1980 | 7194136 |
| serologic studies of naturally acquired infection with vibrio cholerae serogroup o1 in the united states. | the discovery of 11 persons infected with vibrio cholerae serogroup o1 (biotype el tor, serotype inaba) in southwestern louisiana in 1978 provided an opportunity to evaluate the serologic response to this agent in north americans with naturally acquired infection. one antibacterial assay (vibriocidal assay) and two antitoxin assays (enzyme linked immunosorbent assays [elisa] and rabbit skin permeability factor assay) were used. antitoxin levels were elevated longer than vibriocidal antibody leve ... | 1981 | 7194358 |
| structural polypeptides of cholera bacteriophage phi 2. | the structural polypeptides of the cholera bacteriophage phi 2 have been analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. eight different polypeptides were identified. the apparent molecular weights of the polypeptides were 143 000, 96 500, 68 000, 53 000, 37 500, 29 500, 21 000, and 13 500, respectively. the percentage of total protein corresponding to each polypeptide was estimated. | 1981 | 7214233 |
| general transduction in vibrio cholerae. | evidence was obtained for general transduction in vibrio cholerae. transduction of three amino acid markers and three antibiotic resistance characters was demonstrated using strains of biotype eltor and biotype cholerae. some of the genetic characters were transduced from a biotype eltor donor (and its mutant derivatives) to biotype cholerae and eltor recipients. for the genetic traits examined, the frequencies of transduction ranged between 10(-5) and 10(-8). maximal frequencies were obtained w ... | 1981 | 7216473 |
| antibacterial and antitoxin responses in the serum and milk of cholera patients. | antibacterial and antitoxin responses in the acute and convalescent (7 to 10 days) sera of 14 cholera patients were determined by various serological techniques. similar studies were also carried out with corresponding milk samples of six of these patients who were lactating women. a significant rise in antibacterial titers was observed in all convalescent serum and milk samples. a similar rise in antitoxin titers was observable in all serum and four milk samples. specificity of the antibacteria ... | 1981 | 7216479 |
| evaluation of the biological properties of different classes of human antibodies in relation to cholera. | different classes of immunoglobulins (immunoglobulin m [igm], igg, and secretory iga) were purified from pooled serum and milk samples of convalescent cholera patients by gel filtration and immunoadsorbent techniques. the purity of these preparations was established by immunodiffusion and the enzyme-linked immunosorbent assay, using class-specific antisera. the biological properties of antibodies present in these crude and purified immunoglobulin preparations were evaluated by tests related to c ... | 1981 | 7216497 |
| enterotoxicity of group f vibrios. | recently a group of organisms resembling aeromonas hydrophila has been isolated from human diarrheal cases from marine and estuarine environment. these organisms are tentatively designated as group "f" vibrios. live cells of 23 strains isolated from different sources were tested for their enterotoxicity in the adult rabbit ileal loop model. all but two caused fluid accumulation comparable to that obtained with vibrio cholerae 569b. an inoculum of 10(4) viable bacteria could give rise to gut loop ... | 1980 | 7218576 |
| immune responses of rats to live vibrio cholerae: antibodies in serum and intestinal secretions. | following injection of live vibrio cholerae into the small intestine of rats, antibodies appear in the serum and mucus secretions associated with the intestinal surfaces. in contrast to oral immunization, single pulses given by this route cause primary and secondary agglutinating and vibriocidal antibody responses; they are slower to develop but similar to those induced by intravenous injection of 10-fold lower doses of the organisms. however, the intestinal route of injection appears to favour ... | 1981 | 7220079 |
| [electron microscopic study of the structural organization of cholera phage c]. | electron microscopy methods were used to determine the main parameters of the structural organization of cholera "c" phage. the particles of the phage were found to consist of a capsid of icosahedral shape and a thin noncontractile process. the capsid with the triangulation number 7 consists of 72 morphological subunits and contains dna with the molecular weight of (68.16 +/- 1.63) x 10(6) daltons. the process of the phage consists of 216 morphological subunits and ends with a peculiar adsorptio ... | 1980 | 7222634 |
| a study on vibrio cholerae strains isolated in tamil nadu during 1976-79. | 1980 | 7228170 | |
| improved method for cholera diagnosis. | the use of the oxidase test is proposed to discover sparse colonies of cholera vibrios on a plate of pril nutrient agar seeded directly with the suspected stool sample or after enrichment. this method also enables the detection of other oxidase-positive potential pathogens, such as non-agglutinating vibrios, aeromonas hydrophila, pseudomonas spp., and other nonfermentative organisms. | 1981 | 7229013 |
| a non-pathogenic vibrio for the routine quality control of tcbs cholera medium. | a non-pathogenic "indicator" organism to relapse vibrio cholerae in the routine quality control of tcbs medium was sought among a large collection of freeze-dried vibrios isolated mostly from environmental sources. one strain, which was consistently more sensitive to inhibition of growth on tcbs medium that strains of v. cholerae and v. parahaemolyticus, is recommended for this purpose. it has been deposited with, and is available from, the national collection of type cultures as nctc 11218. | 1981 | 7229102 |
| isolation of salmonellae from wall lizards and non agglutinating vibrios from frogs. | 1980 | 7229312 | |
| vibrio cholerae meningitis in a neonate. | 1981 | 7229800 | |
| bcg versus vcn: the antigenicity and the adjuvant effect of both compounds. | enzymatically active vibrio cholerae neuraminidase (vcn) acts as an adjuvant when it is combined with an antigen. this has been demonstrated for bacterial, viral, protein, and cellular antigens and was found for the humoral, but more pronounced for the cellular immune response. in this study, the adjuvant activity of vcn for live bcg is discussed. live bcg itself is regarded as a rather potent immunostimulator. a total of 6.5 mu of vcn, being most effective as derived from previous adjuvant expe ... | 1980 | 7232840 |
| salmonella and other pathogenic bacteria. | the bacterial origin of waterborne diseases was discovered at the turn of this century. introduction of slow sandfiltration, chlorination, and bacteriological control dates back to the same period. although greater concern is given to-day to chemical pollutants or to viruses, bacteria are still a menace to countries with advanced water treatment. within the last decade outbreaks were reported in europe and the us due to salmonella types, shigella, e. coli, and to vibrio cholerae, generally due t ... | 1981 | 7233156 |
| non-o group 1 vibrio cholerae gastroenteritis in the united states: clinical, epidemiologic, and laboratory characteristics of sporadic cases. | fourteen sporadic cases of non-o group 1 vibrio cholerae gastroenteritis were identified through isolates submitted to the centers for disease control in 1979. all the ill persons had diarrhea, 13 had abdominal cramps, 10 had fever, and three had vomiting; in four cases the patients had bloody diarrhea. five patients had traveled outside the united states before they became ill. all nine domestically acquired cases were in patients who had eaten raw oysters within 72 hours of onset of illness; i ... | 1981 | 7235397 |
| occurrence of vibrio cholerae serotype o1 in maryland and louisiana estuaries. | vibrio cholerae serotype o1 has been isolated from chesapeake bay in maryland and estuaries and sewers in louisiana. the occurrence of v. cholerae o1 in the aquatic environment in the absence of human disease suggests that this organism survives and multiples in the natural environment. | 1981 | 7235699 |
| isolation of vibrio cholerae serotype o1 from the eastern oyster, crassostrea virginica. | two strains of vibrio cholerae serotype o1 inaba were isolated from eastern oysters, crassostrea virginica, collected from estuarine waters in florida during april 1980. the oyster meats and waters from which the oysters were collected had low fecal coliform counts, and the area had no prior evidence of sewage contamination. | 1981 | 7235700 |
| a correlation between cell surface sialyltransferase, sialic acid, and glycosidase activities and the implantability of b16 murine melanoma. | a murine melanoma variant (b16-f10ir6), resistant to lymphocytic cytolysis, has been shown previously to produce lower numbers of tumor nodules in the lung of c57bl/6j mice following i.v. inoculations. these differences found in tumor implantation and lymphocyte recognition may be due to changes in surface properties of this cell line. therefore, membrane-bound sialic acid (released by vibrio cholerae neuraminidase treatment), ectosialyltransferase activity, and total cellular glycosidase levels ... | 1981 | 7237426 |
| the chemical structure of the lipid a component of lipopolysaccharides from vibrio cholerae. | the chemical structure of the lipid a component of the lipopolysaccharide from vibrio cholerae 95r was studied. after sequential degradation a reduced d-glucosamine disaccharide was isolated from lipid a and, after permethylation, shown by combined gas-liquid chromatography/mass spectrometry to be beta 1,6-linked. the disaccharide is substituted with a phosphate group, ester-bound to the non-reducing glucosamine (glcn) residue and a pyrophosphorylethanolamine group (pp-etn) linked to c-1 of the ... | 1981 | 7238513 |
| isolation and characterization of a precursor form of the 'a' subunit of cholera toxin. | 1981 | 7238869 | |
| effect of temperature on gene transfer in vibrio cholerae: crosses between p+ & p- classical strains of v. cholerae. | 1981 | 7239566 | |
| immune responses of rats to live vibrio cholerae: secretion of antibodies in bile. | agglutinating and vibriocidal antibodies appear in the bile of rats within a few days of intravenous or intraintestinal injection of live vibrio cholerae. whereas the presence of igm and igg antibodies area accounted for by passive exudation from serum., those of the iga class are selectively (actively) transferred from serum to bile. bile duct ligation causes a 10-fold increase in serum iga levels over a 48-h period; a similar increase in agglutinating antibody titre occurs only in rats injecte ... | 1981 | 7243338 |
| [current data on diarrhea of vibrio etiology. i. current studies on diarrhea caused by vibrio cholerae 0:1]. | cholera, a problem of public health, as well as the other diarrheas included in the notion of diarrheic disease of infectious aetiology, has been the subject during the last two decades of extensive investigations, whose results tend to modify classical knowledge and therefore the mode of understanding of the diarrheic disease in general and of cholera in particular. the authors review recent progress in the domain of taxonomy, pathogeny (enterotoxins-immunodeterminants), sensitivity to antibiot ... | 1980 | 7244509 |
| characterization of vibrio cholerae isolated from oysters. | of 790 samples of oyster shellstock freshly harvested during a 12-month survey, 111 (most of which were harvested from june through august) contained vibrio cholerae non-o1 (611 strains), and seven contained o1 inaba (11 strains) organisms. none of the v. cholerae strains isolated were enterotoxigenic by immunological and biological tests. | 1981 | 7247399 |
| survival of vibrio cholerae in water. | 1980 | 7248589 | |
| characterization and use of neuraminidase-modified l1210 plasma membranes for protection against tumor growth. | purified plasma membranes were prepared from l1210 ascites tumor cells and analyzed for their protein and carbohydrate composition. conditions were developed for treating the isolated plasma membranes with vibrio cholerae neuraminidase (vcn) so that 88% of the n-acetylneuraminic acid was removed without changing membrane proteins or other membrane carbohydrate constituents. the vcn-induced modifications were characterized by labeling vcn-treated and untreated l1210 cells by the galactose oxidase ... | 1981 | 7248964 |
| immunotherapy of l1210 leukemia using neuraminidase-modified plasma membranes combined with chemotherapy. | purified l1210 plasma membranes treated with vibrio cholerae neuraminidase (vcn) were used for active immunotherapy of l1210 tumors in dba/2j mice. immunotherapy with vcn-treated membranes was effective only when combined with 1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea (meccnu). successful therapy was a function of the dose of meccnu, the dose of vcn-treated membranes, and the time after meccnu treatment when vcn-treated membranes were administered. optimum conditions for treating an ... | 1981 | 7248965 |
| biochemical studies on lipopolysaccharide isolated from agglutinable & non-agglutinable strains of v. cholerae. | 1980 | 7251036 | |
| ability of an avirulent mutant of vibrio cholerae to colonize in the infant mouse upper bowel. | vibrio cholerae strain 3083 (biotype el tor, serotype ogawa) and texas star-sr (sr), a mutant derived from 3083 that produces the b (binding) but not the a (toxic) subunit of choleragen, were compared in their abilities to: (i) associate with the infant mouse upper bowel; (ii) survive and multiple there; and (iii) induce diarrhea. vibrios labeled with 35so4 were used to determine association with the upper bowel and ability to multiply. the parental strain associated significantly better than sr ... | 1981 | 7251134 |
| molecular characterization of environmental and nontoxigenic strains of vibrio cholerae. | environmental and nontoxigenic strains of vibrio cholerae 0-1 were examined for genes homologous to genes encoding escherichia coli heat-labile enterotoxin (lt). restriction fragments encoding lt a and b subunits were isolated from the recombinant plasmid ewd299 and labeled in vitro with 32p. these probes were then hybridized to deoxyribonucleic acid extracted from strains of v. cholerae and visualized by autoradiography. none of the nontoxigenic strains of v. cholerae 0-1 from louisiana, alabam ... | 1981 | 7251142 |
| duration of infection-derived immunity to cholera. | four volunteers were rechallenged with vibrio cholerae (10(6) classical ogawa 395 organisms) 33-36 months after their initial induced cholera infection; none of the four veterans and four of five control volunteers developed diarrhea (p = 0.04). all control subjects, but only one veteran, had positive coprocultures. three of the four veterans had significant levels of serum igg antitoxin before challenge, but none had measurable intestinal levels of secretory iga antitoxin. significant rises in ... | 1981 | 7252264 |
| the isolation of vibrio alginolyticus from a patient with acute entero-colitis. | vibrio alginolyticus was isolated from the "rice water" diarrheal stool of a female patient with acute entero-colitis, and from the trout roe which she ate. subsequently, it was clearly demonstrated that, besides of vibrio cholerae and vibrio parahaemolyticus, vibrio alginolyticus was also enteropathogenic for humans. additionally, we described the difference in the colony formation on some commercial thiosulfate citrate bile salts sucrose (tcbs) agars when vibrio alginolyticus and vibrio choler ... | 1980 | 7256728 |
| abo blood group distributions in diarrhoea cases including cholera in calcutta. | a double blind study on determination of abo blood groups of 210 cholera patients, 44 diarrhoea cases due to vibrio parahaemolyticus, and 148 diarrhoea cases from whom no vibrios could be isolated (control group), was conducted in calcutta. a statistically significant difference was found in blood group 'o' when comparison was made between control and cholera groups. no significant association was, however, notices between v. parahaemolyticus-induced diarrhoea and abo blood groups. | 1981 | 7259106 |
| the role of trace elements and phosphates in the synthesis of vascular-permeability factor by vibrio cholerae. | trace element and phosphate requirements for the synthesis of vascular-permeability factor (pf) by vibrio cholerae strains b1307 and vc12 were investigated. while magnesium appeared to be indispensable for strain vc12, small amounts of pf were synthesised by strain b1307 in the presence of iron, zinc and manganese. however, even in the latter strain, maximum synthesis was recorded only in magnesium-containing media. phosphates in the range 0.75-6.00mm controlled the synthesis of pf by both strai ... | 1981 | 7265164 |
| [2 cases of septicemia caused by vibrio non cholerae or non-o1 vibrio cholerae]. | 1981 | 7267357 | |
| [examination of the antibacterial action of metronidazole against vibrios and campylobacter (author's transl)]. | the in vitro antibacterial activity of metronidazole was tested against 70 strains of aerobic vibrios (v. cholerae biotype cholerae, v. cholerae biotype eltor, nag-vibrios, v. parahaemolyticus, v. alginolyticus) and 30 strains of microaerophilic campylobacter (c. fetus subsp. fetus, c. fetus subsp. intestinalis and c. fetus subsp. jejuni). all strains of aerobic vibrios proved to be resistant (mic 100 micrograms/ml) in contrast to campylobacter strains which were sensitive (mic 1-4 micrograms/ml ... | 1981 | 7269853 |
| [immunochemical heterogeneity of vibrio cholerae eltor lipopolysaccharides and methods of fractionating them]. | 1981 | 7269926 | |
| [activity of fourteen antibiotics on vibrio cholerae el tor]. | two hundred and one strains of vibrio cholerae el tor were tested by agar diffusion technic against fourteen antimicrobial agents. all the strains tested are inhibited by tetracyclines, gentamycin, tobramycin, kanamycin, chloramphénicol, carbenicillin and the association of trimétomycin but mainly the colistin are the less active. a great majority of strains are inhibited by the ampicillin. the streptomycin but mainly the colistin are the less active. | 1980 | 7271371 |
| [morphofunctional changes in the intestines of rabbits as affected by cholera vibrio toxins]. | forty adult rabbits were inoculated with cholera vibrio toxins (endotoxin and enterotoxin) intravenously or into the operatively pre-isolated segments of the jejunum some of which remained intact. the examinations were carried out 3--5 hours after intravenous and 18--24 hours after intraintestinal inoculation of the toxins. irrespective of the route of endotoxin inoculation, circulation disorders developed predominantly in the jejunum mucosa. its content did not change in volume but the potassiu ... | 1981 | 7271508 |
| a non-o1 vibrio cholerae isolated from a goose. | 1981 | 7271657 | |
| inhibition of mouse natural killer activity by cholera toxin. | the treatment of mouse spleen cells with cholera toxin (ct) extracted from vibrio cholerae greatly reduced natural killer (nk) activity. the inhibition of nk activity was rapidly induced by ct treatment and persisted in the absence of ct. even a very small amount of ct, such as 0.1 ng/ml, was effective in inhibiting nk activity. the inhibitory effect of ct on nk activity was lost upon preabsorption of ct with brain tissues, but not upon preabsorption with liver or kidney tissues. | 1981 | 7274638 |
| virulence of three clinical isolates of vibrio cholerae non-o-1 serogroup in experimental enteric infections in rabbits. | three vibrio cholerae, [ill] isolates, pathogenic in both adult rabbit ligated ileal loop and infant rabbit assays, produced no heat-labile or heat-stable toxins. their pathogenicity did not correlate with the presence of plasmids. | 1981 | 7275319 |
| fk phage for differentiating the classical and el t or groups of vibrio cholerae. | a new vibrio-infecting phage (fk phage) isolated from sewage lysed all strains of vibrio cholerae biovar cholerae, whereas all strains of v. cholerae biovar el tor were resistant to it. fk phage was entirely different from mukerjee group iv phage in morphology and antigenicity. in addition to group iv phage, the use of fk phage will be useful in the examination and typing of v. cholerae. | 1981 | 7276149 |
| lysogenicity of the strains of vibrio cholerae, biotype eltor recently isolated in various countries. | 1981 | 7278707 | |
| [genetic control of toxin formation by vibrio cholerae. i. mobilization of the tox gene of vibrio cholerae by plasmid rp::mu ctss62]. | the possibility of the mobilization of v. cholerae genes responsible for toxin formation (tox gene) and serological specificity (oag-i gene, serotype inaba) by the rp4::mu cts62 plasmid was established. the cotransfer of tox and oag-i genes with his and ilv genes respectively by means of the rp4::mu cts62 plasmid was revealed. the frequency of the cotransfer of tox his and oag-i ilv observed in our experiments corresponded to the known frequency of the cotransfer of these genes observed in cross ... | 1981 | 7282188 |
| vibrio infections in singapore. | both symptomatic and asymptomatic vibrio infections, caused by v cholerae, v parahaemolyticus and nag vibrios, occurred in singapore. v cholerae are introduced into the country from time to time, but the risk of transmission of infection in the community is negligible in the presence of a high standard of environmental sanitation and a well-established system of epidemiological surveillance. as cholera is probably transmitted through contaminated food, shellfish imported from cholera-endemic cou ... | 1981 | 7283382 |
| [morphological study of the effect of cholera toxin on germ-free animals]. | the effect of purified cholera toxin and the o-antigen of vibrio cholerae inoculated separately into the intestines of germ-free suckling rabbits on the structure of the viscera and enzymatic changes in them was studied. the purified cholera toxin exerted mostly a local effect on the intestinal tract but caused lesions in the lymphoid tissue. the o-antigen was toxic causing primarily lesions in the liver and kidneys. crude cholera toxin exerted a complex effect on gnotobiotic rabbits typical of ... | 1981 | 7283785 |
| repair of ultraviolet-light-induced dna damage in vibrio cholerae. | repair of ultraviolet-light-induced dna damage in a highly pathogenic gram-negative bacterium, vibrio cholerae, has been examined. all three strains of v. cholerae belonging to two serotypes, inaba and ogawa, are very sensitive to ultraviolet irradiation, having inactivation cross-sections ranging from 0.18 to 0.24 m2/j. although these cells are proficient in repairing the dna damage by a photoreactivation mechanism, they do not possess efficient dark repair systems. the mild toxinogenic strain ... | 1981 | 7284396 |
| sequential combination chemoimmunotherapy for various malignant tumors: clinical and laboratory results. | a chemoimmunotherapy program designed on the bases of the results of animal experiments was designed for 139 patients with various advanced malignant tumors. the treatment regimen consisted of cyclophosphamide (cy) 200 mg intravenously on day 1, vibrio cholerae neuraminidase (vcn) treated autologous tumor cells admixed with bcg 5 to 10 mg intradermally on day 4 and mitomycin c (mmc) 10 to 16 mg and 5-fluorouracil (fu) 500 mg intravenously on day 7, of each course. thereafter, maintenance treatme ... | 1981 | 7289236 |
| [8-year survival of a vibrio with altered morphology on a medium with added petroleum growth substance]. | 1981 | 7293576 | |
| uptake of vibrio cholerae biotype eltor from contaminated water by water hyacinth (eichornia crassipes). | vibrio cholerae biotype eltor appears to concentrate on the surface of the water hyacinth (eichornia crassipes), thereby enhancing its survival and its potential for transmission through waterways of cholera-endemic regions such as bangladesh. | 1981 | 7294788 |
| partial characterization of sialoglycopeptides produced by cultured human melanoma cells and melanocytes. | the sialoglycopeptides produced by hm7 human melanoma and fetal uveal melanocyte cultures grown in the presence of [3h]glucosamine and [35s]sulfate were isolated from the pronase digests of cells, spent media, and intracellular material. from the melanoma culture, six sialoglycopeptides, accounting for 43% of the total 3h radioactivity in the nondiffusible cell-associated glycopeptides, were purified. a major glycopeptide (gpib) having an apparent molecular weight in the range 12 000-15 000 show ... | 1981 | 7295695 |
| radiomimetic property of furazolidone and the caffeine enhancement of its lethal action on the vibrios. | sensitivities of the strains belonging to four vibrio biotypes to the action of furazolidone were investigated. vibrio cholerae (classical) was most and vibrio parahaemolyticus least sensitive to this drug. statistical analyses revealed significant differences between any two of the four types of vibrio in respect of their sensitivity to furazolidone. the drug was radiomimetic in action, the doses of uv light (duv) and furazolidone (df) required for 10% survival of the vibrios being correlated b ... | 1981 | 7296700 |
| role of chemotaxis in the association of motile bacteria with intestinal mucosa: chemotactic responses of vibrio cholerae and description of motile nonchemotactic mutants. | a motile, chemotactic, ogawa strain of vibrio cholerae was attracted by all 20 l-amino acids tested, in contrast to escherichia coli aw 405, which did not react to several of these. the maximum number of vibrios entering a capillary was much lower when the capillary contained carbohydrates rather than amino acids, but the minimum effective concentrations of the carbohydrates and amino acids tested were of the same order of magnitude. l-fucose, a sugar known to inhibit the adhesion of this vibrio ... | 1981 | 7298183 |
| role of chemotaxis in the association of motile bacteria with intestinal mucosa: fitness and virulence of nonchemotactic vibrio cholerae mutants in infant mice. | contrary to earlier findings with all other in vivo and in vitro models of cholera studied, nonchemotactic vibrio mutants showed a relatively greater fitness in 5-day-old infant mice as compared with chemotactic parent or chemotactic revertant strains. this trend was manifest in the relatively greater number of nonchemotactic mutants recovered from the upper small intestine at 4 and 18 h after intragastric infection. the same trend was also revealed in the significantly greater virulence (in ter ... | 1981 | 7298184 |
| role of chemotaxis in the association of motile bacteria with intestinal mucosa: in vivo studies. | in vivo loops were prepared in the small intestine of rabbits and injected with mixtures of vibrio cholerae and polystyrene spheres (1.1-micrometers diameter). the loops were removed and frozen after 15 min and then sectioned in a cryostat. the locations of particles and vibrios were determined microscopically. the vibrio/particle ratio was unity in the lumen of the loops, but increased 10-fold in the deep intervillous spaces, indicating active invasion of the mucus gel by the chemotactic parent ... | 1981 | 7298185 |
| role of chemotaxis in the association of motile bacteria with intestinal mucosa: in vitro studies. | various sephadex g-15 fractions of pepsin-digested mucosal extract inhibited the in vitro association of cholera vibrios with mucosal slices. inhibitory activity paralleled the taxin activity of the fractions for these bacteria. this supports the theory that inhibition of mucosal association by pepsin-digested mucosal scrapings was due to the blocking of taxin receptors on the bacterial surface. nonchemotactic mutants were significantly less efficient than the chemotactic parent or revertant str ... | 1981 | 7298186 |
| experimental cholera in germfree suckling mice. | vibrio cholerae v86 el tor inaba caused experimental cholera in germfree icr suckling mice. sucklings born of gnotobiotic mothers monoassociated with the same strain were completely protected against live oral homologous challenge. | 1981 | 7298190 |
| production of cholera-like enterotoxin by a vibrio cholerae non-o1 strain isolated from the environment. | vibrio cholerae non-o1 strain e8498, isolated in 1978 from fresh water in louisiana, produced a vascular permeability factor when cultured in shallow resting cultures of casamino acids-yeast extract-glucose medium for 24 h at 30 degrees c. undiluted resting culture filtrates contained heat-labile permeability factor activity which was only partially neutralized by cholera antitoxin and gm1 ganglioside. supernatants concentrated with pm-10 membranes caused hemorrhage and necrosis in rabbits withi ... | 1981 | 7298194 |
| a case of shellfish associated cholera in south florida. | 1981 | 7299364 | |
| proliferation of enteropathogens in oral rehydration solutions prepared with river water from honduras and surinam. | oral rehydration of infants with diarrhoea is an effective therapy that is becoming increasingly available in developing countries. to formulate judicious recommendations for preparation and storage of such solutions, we assessed the capability of recognized bacterial enteropathogens to survive and proliferate in solutions made either with sterile distilled or river water collected in two developing countries. shigella flexneri, an enteropathogen typically transmitted by faecal/oral contact rath ... | 1981 | 7299876 |
| [mutations of antibiotic resistance in vibrios under gamma irradiation]. | 1981 | 7302156 | |
| molecular properties of cholera enteroxin. | the molecular properties of vibrio cholerae enterotoxin obtained from two different insitutions were studied. the preparations, which were purified by different methods, showed the same physicochemical properties. ultracentrifugal analysis showed that the toxin had a molecular weight of 79,000, a sedimentation coefficient of 5.25 s and a diffusion coefficient of 6.02 d. when the toxin was subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, the a subunit wit ... | 1981 | 7303003 |
| the antigenic variation of vibrio cholerae in a newly-infected developing country. | 1981 | 7303139 | |
| non-o group 1 vibrio cholerae gastroenteritis associated with eating raw oysters. | a cluster of five cases of non-o group 1 (non-o1) v. cholerae gastroenteritis occurred in one florida locality during november 1979. clinical findings included nausea, vomiting, and abdominal cramping in all affected persons; two had bloody diarrhea. all five persons gave a history of eating raw oysters within four days of onset of illness. a case-control study statistically associated the eating of raw oysters with development of illness (p = 0.0008); this finding was confirmed by a retrospecti ... | 1981 | 7304565 |
| duration of serum antitoxin response following vibrio cholerae infection in north americans: relevance for seroepidemiology. | 1981 | 7304570 | |
| study to determine duration of vibrio cholerae excretion in malawians and to test efficacy of treatment. | 1981 | 7307069 | |
| phagocytosis of sialidase-treated rat erythrocytes: evidence for a two-step mechanism. | binding and phagocytosis of rat erythrocytes by liver and peritoneal macrophages were studied with a radioactive in vitro assay which yields quantitative data. partial removal of sialic acids from the erythrocytes by vibrio cholerae sialidase resulted in a marked increase of binding of the red cells by both liver and unstimulated peritoneal macrophages. peritoneal macrophages stimulated by thioglycolate or starch, however, did not differentiate between control and desialylated erythrocytes. by i ... | 1981 | 7309007 |
| [epidemiological characteristics of cholera e1/or in cases of predominantly water and household modes of disease transmission]. | 1981 | 7315018 | |
| sialidase treatment of rat-erythrocytes: a model for cellular aging? | an in vitro assay system was developed to test the propensity of "old" and sialidase-treated rat erythrocytes to be bound and phagocytosed by rat peritoneal macrophages. cells considered to be old were phagocytosed to a greater degree than those considered to be young. when erythrocytes were treated with immobilized vibrio cholerae sialidase, higher amounts of sialic acids had to be removed (25--30%) to induce binding and phagocytosis of the cells, than if the cells had been treated with soluble ... | 1981 | 7315090 |
| polyacrylamide gel electrophoresis and infrared spectroscopy of vibrio biotypes. | polyacrylamide gel electrophoresis and infrared spectroscopy were used to study the relationship between vibrio cholerae (classical), vibrio cholerae (el tor), and nonagglutinable (nag) vibrios. acid extracts of the different strains produced type-specific electrophoretic patterns, and the infrared spectra revealed broad absorption maxima which largely correspond to those found in other organisms. with the exception of the nag vibrios, the infrared spectra of cholera el tor vibrios were identica ... | 1981 | 7317857 |
| [taxonomic position of representatives of the species vibrio cholerae]. | the data contained in the works of some foreign authors who have reconsidered the taxonomic position of v. metschnikovii are analyzed. in contrast to the views expressed by gamaleya whi believed this microorganism to be the modified cholera vibrio, the foreign authors identify it with the "kommabacillus der cholera nostras" described by finkler and prior (1884) and classify it with saprophytes widely spread in the environment. these points of view were refuted by the data resulting from the stud ... | 1981 | 7324652 |
| [o-antigen of vibrio cholerae, serovar ogawa, recommended for the production of oral cholera bivalent vaccine]. | 1981 | 7324656 | |
| a vibrio cholerae infection in a transient teamster. | an illness indistinguishable clinically from classical cholera but caused by a non-cholera vibrio occurred in an over-the-road truck driver. the infecting organism was not finally identified as vibrio cholera, smith serotype 113 toxin positive, until 4 weeks after his hospital discharge. hospital laboratories in most parts of the united states are unlikely to identify vibrio cholerae in stool cultures unless specifically requested to do so. if one recognizes the chronic cholera carriers have bee ... | 1981 | 7325285 |
| the release of n-acetyl- and n-glycolloyl-neuraminic acid from soluble complex carbohydrates and erythrocytes by bacterial, viral and mammalian sialidases. | a series of substrates, sialyl(2 leads to 6)galnac and ganglioside gm3, containing either n-acetylneuraminic acid (acneu) or n-glycolloylneuraminic acid (gcneu), has been prepared. the trisaccharide gcneu(2 leads to 3)lactose was preapred by ozonolysis of gcneu-gm3, and the disaccharides acneu(2 leads to 6)galnac and gcneu(2 leads to 6)galnac were isolated from bovine submandibular-gland mucin by alkali elimination. sialidases from newcastle-disease virus, fowl-plague virus, influenza virus a2, ... | 1981 | 7325957 |
| [transferable r plasmids isolated from vibrio cholerae (author's transl)]. | 1981 | 7328757 | |
| [current status of diarrhea caused by or associated with cholera vibrions of the non 0:1 group]. | known until not long age under the generic name of non-agglutinable vibrios (nag), cholera vibrios group non 0:1 represent a group as yet insufficiently known in spite of the marked progress made during the last 10 - 15 years in this field. wide spread throughout the world vibrios group non 0:1 have been isolated from surface waters, from the intestines of fish, birds and mammals (from humans in case of enteritis, systemic infections, healthy subjects). the medical interest stands in the partici ... | 1981 | 7330562 |
| [use of the human lymphocyte transformation test to detect a biologically active cholera toxin in vibrio cholerae culture filtrates]. | 1981 | 7331494 | |
| [production of an erythrocytic ganglioside diagnostic reagent for detecting cholera enterotoxin and toxoid in indirect hemagglutination tests]. | the studies described in this work have indicated that cholera enterotoxin and its components (cholerogenoid and subunit b) can be detected in amounts of 0.25, 0.28 and 0.6 microgram of protein per 1 ml, respectively, by means of erythrocytes sensitized with gangliozide-containing complex. the conditions for erythrocyte sensitization have been established. the methods of cholerogen titration in the passive hemagglutination test by means of the erythrocytic gangliozide diagnostic reagent and in c ... | 1981 | 7331599 |
| [preservation of certain biological properties of vibrio cholerae el tor in river and sewage water following aeration]. | 1981 | 7331603 | |
| [evaluation of the adhesion, colonization and enterotoxigenicity of vibrio cholerae in the enteral infection of intact and passively immunized newborn animals]. | v. eltor were introduced into 323 suckling rabbits through a gastro-duodenal tube and into 629 suckling mice either by the same method, or per annum by a new specially developed technique. passive immunization was achieved in suckling mice by the preliminary introduction of antibacterial sera into the small intestine or by feeding them with the milk of female mice immunized at the end of pregnancy, and in suckling rabbits by feeding them with the milk of goats immunized by introducing vibrios th ... | 1981 | 7331624 |
| factors affecting phaeomelanin production by a melanin-producing (mel) mutant of vibrio cholerae. | in a previous study we isolated melanin-producing (mel) mutants of vibrio cholerae and demonstrated that production of melanin during growth on solid media was stimulated by l-tyrosine and l-cysteine. in the studies reported here we analyzed factors that affected melanin production in liquid media and determined the abilities of radioactively labeled amino acids to serve as precursors for the formation of melanin by v. cholerae. radioactivity from l-cysteine and from l-tyrosine was preferentiall ... | 1981 | 7333674 |
| an electrophoretic analysis of variation in the glucose-6-phosphate dehydrogenase and malate dehydrogenase of vibrio cholerae, vibrio parahaemolyticus and vibrio fluvialis. | 1981 | 7333985 | |
| adhesion of vibrios and aeromonads to isolated rabbit brush borders. | isolated rabbit brush borders were used to investigate the adhesive properties of clinical and environmental isolates of non-o1 vibrio cholerae and clinical isolates of vibrio parahaemolyticus and aeromonas hydrophila. a minority of the isolates were found to adhere to brush borders. the adhesion of these isolates was affected by a number of factors, including the bacteria:brush border ratio, incubation time, culture medium and growth temperature. adhesion was inhibited by l-fucose but was indep ... | 1981 | 7334329 |
| gauze filtration and enrichment procedures for recovery of vibrio cholerae from contaminated waters. | gauze filtration followed by 18-h enrichment in alkaline bile-peptone water is a simple, inexpensive, and efficient method for isolation vibrio cholerae biotype eltor from contaminated surface waters. | 1981 | 7337468 |
| vibrio cholerae non group o:1 associated with middle ear infection. | 1981 | 7340725 | |
| protection against vibrio cholerae infection afforded by fragments of anti-haemagglutinin. | the finding that fab fragments of the anti-e1 tor haemagglutinin were able to afford protection in vivo (low but significant), as well as a significant reduction in vibrio cholerae adherence to isolated intestinal epithelial cells in-vitro, implicate that masking of these cell-bound haemagglutinin sites per se, would be sufficient to confer protection in e1 tor cholera infection. subsequently, the related working hypothesis that the e1 tor cell-bound haemagglutinin is playing an adhesive role is ... | 1981 | 7344098 |
| time of maximum colonisation by vibrio cholerae el tor in 5-6 day old mice as an experimental cholera model. | 1981 | 7344115 | |
| [glycomacropeptide from whey: a substrate for determining the activity of bacterial neuraminidases]. | 1981 | 7345910 | |
| association of vibrio cholerae mutants with the intestinal mucosa of infant mice. | scanning electron microscopy (sem) was used to observe the nature of association of mutant strains of vibrio cholerae to infant mouse intestinal segments. parental strain inaba ca 401 was compared to the following mutant phenotypes: nonchemotactic, nonchemotactic reduced motility, flagellated nonmotile, nonflagellated nonmotile, and mucin nonutilizing. the virulence of the different strains was determined by oral-intra-gastric inoculation of seven-day-old mice. localization of the parental strai ... | 1981 | 7347415 |
| cholera--a possible endemic focus in the united states. | in september and october 1978, after a case of cholera had been discovered in southwestern louisiana, 10 more vibrio cholerae o-group 1 infections were detected in four additional clusters. all 11 infected persons had recently eaten cooked crabs from five widely separated sites in the coastal marsh, and a matched-triplet case-control study showed a significant relation between cholera and eating such crabs (p = 0.007). v. cholerae o1 was isolated from estuarine water, from fresh shrimp, from a l ... | 1980 | 7350497 |
| differences between the o-acetylated sialic acids of the epithelial mucins of human colonic tumors and normal controls: a correlative chemical and histochemical study. | chemical analyses, together with histochemical assessments, were carried out on specimens of adenocarcinoma of the colon and histologically normal colonic epithelium (from resection margins from cases of carcinoma of the colon). in the epithelial glycoproteins of the normal tissue, both chemical and histochemical investigations indicated that the great majority of the sialic acids contained a side-chain o-acyl substituent located at position c8, whereas the side-chain substitution of the sialic ... | 1980 | 7354217 |
| immunochemical studies of the lipopolysaccharides of vibrio cholerae: constitution of o specific side chain and core polysaccharide. | lipopolysaccharides from various strains of vibrio cholerae have been found to consist of distinct o-specific side chain and core polysaccharide regions in their degraded polysaccharides. the major identifiable components in the core polysaccharide were phosphorus, glucose, heptose, fructose, and ethanolamine phosphate, with small amounts or traces of mannose, rhamnose, and d-perosamine. on the other hand, glucose, fructose, mannose, rhamnose, glucosamine, d-quinovosamine, and d-perosamine were ... | 1980 | 7358427 |
| events in the pathogenesis of experimental cholera: role of bacterial adherence and multiplication. | pathogenic, and laboratory-derived non-adherent, non-motile, streptomycin-dependent and attenuated strains of vibrio cholerae, were injected into the ileal loops of adult rabbits. the pattern of bacterial adherence and multiplication was studied. it was shown that all the strains multiplied to the same extent in the intestine; multiplication per se had no role in pathogenesis except when the infecting dose was low. vibrio strains differed in their capacity to adhere to the intestine. a good corr ... | 1980 | 7359569 |
| "neuraminidase-resistant" sialic acid residues of gangliosides. | 1980 | 7361617 | |
| enzymic degradation of gangliosides. | 1980 | 7361629 | |
| [determination of the taxonomic position of vibrio parahaemolyticus using the technic of molecular hybridization of dna]. | 1980 | 7362850 |