Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [characteristics and function of enterotoxins of gram negative bacteria (author's transl)]. | several factors are important for the pathogenesis of bacterial intestinal disease: 1. colonisation of the intestine; 2. exo(= entero)toxin production without penetration into the intestinal wall; 3. invasion of the intestinal wall with or without (?) simultaneous formation of enterotoxin. these factors are of different importance for the various infective agents. whereas cholera is exclusively caused by an enterotoxin, this is of minor importance for the pathogenesis of shigella dysentery. the ... | 1980 | 7005079 |
| [comparative features of methos of inducing auxotrophic mutants of cholera and nonagglutinating vibrios]. | the effect of chemical mutagens (ei, neu, nmu, nmg) on six el-tor strains and eight nag-vibryos was studied in order to obtain auxotrophic mutatns. the methods used showed the advantage of a. adelberg and d. mayers's method in the yu. g. suchkov's modification. it enables to get a greater number of mutants, and it differs from other methods in the simplicity in organization of experiments. the mutants obtained of el-tor and nac-vibryos differed from each other and from initial strains in their b ... | 1980 | 7007163 |
| current developments with cholera vaccines: "where do we go from here?". | 1980 | 7010372 | |
| immunological properties of the cell envelope components of vibrio cholerae. | several immunobiological properties of cell envelope components of vibrio cholerae such as mitogenicity, antigenicity, adjuvanticity and toxicity were tested in mice. killed whole bacteria, spheroplasts, lipopolysaccharide and outer membrane proteins possessed mitogenic activity as determined by [3h]thymidine uptake in spleen cell cultures. all these components predominantly stimulated murine bone-marrow derived (b) lymphocytes. the mitogenicity induced by v. cholerae lipopolysaccharide was simi ... | 1980 | 7014769 |
| sequence homologies between a subunits of escherichia coli and vibrio cholerae enterotoxins. | the genes coding for the heat-labile enterotoxin lt produced by escherichia coli have been cloned into the plasmid pbr313. using dna derived from the resulting chimeric plasmid, we determined the nucleotide sequence of two regions of the gene coding for the enzymatically active a subunit of lt. translation of the nucleotide sequence gives the primary structure of the nh2-terminal and cooh-terminal regions of the lt a subunit. this permits direct comparison of the lt a subunit with the a subunit ... | 1981 | 7017718 |
| [features of the 7th cholera pandemic]. | 1981 | 7018134 | |
| simple adult rabbit model for vibrio cholerae and enterotoxigenic escherichia coli diarrhea. | we developed an adult rabbit model for enteric infection by vibrio cholerae and enterotoxigenic escherichia coli. the cecum of each animal was first ligated to prevent it from retaining fluid secreted by the small intestine. a temporary reversible obstruction (a slip knot tie) of the small bowel was introduced at the time of challenge and removed 2 h later. with this modification, we were able to elicit a massive and usually fatal cholera-like diarrhea in adult (3.5- to 6-lb [1.6- to 2.7-kb]) an ... | 1981 | 7019078 |
| the immune response of mice to cholera vaccine. | 1981 | 7019423 | |
| actions of cholera toxin and the prevention and treatment of cholera. | the drastic intestinal secretion of fluid and electrolytes that is characteristic of cholera is the result of reasonably well understood cellular and biochemical actions of the toxin secreted by vibrio cholerae. based on this understanding it is possible to devise new techniques for the treatment and prophylaxis of cholera to complement those based on fluid replacement therapy and sanitation. | 1981 | 7019725 |
| [steroid stimulation in adrenal tumor cells with enterotoxins from vibrio cholerae and escherichia coli]. | 1981 | 7019819 | |
| mechanisms of association of bacteria with mucosal surfaces. | bacterial association with host mucosal surfaces involves a large number of steps. successful negotiation of each of these requires -- or is at least facilitated by -- the development of a distinct set of characteristics (virulence factors) by the bacterium. the major steps include: (a) chemotactic attraction of motile bacteria to the surface of the mucus gel, (b) penetration of and trapping within the mucus gel (which may be passive or can be promoted actively by bacterial motility and chemotax ... | 1981 | 7021088 |
| nonimmunoglobulin fraction of human milk inhibits bacterial adhesion (hemagglutination) and enterotoxin binding of escherichia coli and vibrio cholerae. | human milk and colostrum samples were divided into an immunoglobulin and a nonimmunoglobulin fraction by immunosorbent chromatography. the ability of these fractions to inhibit bacterial cell adhesion and enterotoxin receptor binding of vibrio cholerae and various escherichia coli isolates was then tested by in vitro assays. the strongest effect was generally seen with the nonimmunoglobulin fractions, which were shown to significantly inhibit e. coli cell adhesion (hemagglutination) mediated by ... | 1981 | 7021421 |
| [biochemical properties of escherichia, vibrio cholerae, and shigella toxins]. | 1981 | 7023142 | |
| [adhesiveness, invasiveness and enterotoxicity of the agents of intestinal infections]. | 1981 | 7023151 | |
| transfer of r plasmid from staphylococcus aureus to escherichia coli & vibrio cholerae. | 1981 | 7024113 | |
| the stability of r plasmids in vibrio cholerae. | 1980 | 7024610 | |
| environmental aspects of cholera epidemiology. ii. occurrence and survival of vibrio cholerae in the environment. | 1981 | 7027559 | |
| [isolation and characteristics of e. coli plasmid-determined k88 antigen]. | the advantage of ultracentrifugation over isoelectric focusing in the isolation of antigen k88 from e. coli is shown. the production of antigen k88 is reca-independent. the antigen has been found to be nontoxic for mice, to have the isoionic point at ph 4.1 and the sedimentation coefficient 16.6s, to completely consist of protein subunits of 25,000 daltons. the expression of antigen k88 enhances the adhesive properties of the host cell. no relationship between antigen k88 and the supposed adhesi ... | 1981 | 7034412 |
| berberine inhibits intestinal secretory response of vibrio cholerae and escherichia coli enterotoxins. | berberine, an alkaloid from the plant berberis aristata, which has been known since ancient times as an antidiarrheal medication in india and china, inhibited by approximately 70% the secretory responses of the heat-labile enterotoxins of vibrio cholerae and escherichia coli in the rabbit ligated intestinal loop model. the drug was effective when given either before or after enterotoxin binding and when given either intraluminally or parenterally; it did not inhibit the stimulation of adenylate ... | 1982 | 7035365 |
| environmental aspects of cholera epidemiology. iii. transmission and control. | 1982 | 7036486 | |
| evaluation of a test-kit (oxi/ferm system) for identification of vibrios. | a total of 258 strains belonging to different species of vibrio isolated from various sources in different parts of the world were subjected to identification by oxi/ferm tube and conventional tests sometimes extending to 250 characters when the conventional tests failed to recognise them. nearly 88 per cent of them could be identified accurately in this system at the species level. the strains designated as mannitol negative vibrios and vibrio species were also identified properly as v. cholera ... | 1981 | 7036597 |
| [mobilization of vibrio cholerae chromosomal genes by plasmid rp4::mu cts62]. | the rp4::mu cts62 plasmid was constructed in escherichia coli cells and subsequently transferred by conjugation into the cells of vibrio cholerae. this plasmid had been shown to mobilize chromosomal genes of v. cholerae during intrageneric matings. the frequency of mobilization is higher in matings at 37 degrees c, the temperature which is semipermissive for temperature sensitive mu cts62 phage. this is only true for strains harbouring rp4::mu cts62, but not for strains containing the rp4 plasmi ... | 1982 | 7037543 |
| a rapid visual test to characterize cholera vibrios. | 1981 | 7037723 | |
| characterization of biochemically atypical vibrio cholerae strains and designation of a new pathogenic species, vibrio mimicus. | biochemically atypical strains classified as vibrio cholerae were characterized by biochemical reactions, serology, antibiotic susceptibility testing, and deoxyribonucleic acid relatedness. strains with the following atypical reactions were shown to be v. cholerae: mannose negative, mannitol negative, lysine decarboxylase negative, no growth in the presence of 5% nacl, salicin and cellobiose positive. sucrose-negative strains were shown to constitute a new species, vibrio mimicus, whose type str ... | 1981 | 7037833 |
| [new method of preparing antibody immunosorbents for nonprotein antigens]. | 1981 | 7039098 | |
| survival of vibrio cholerae and escherichia coli in estuarine waters and sediments. | in in vitro estuarine water and sediment chambers, the survival of vibrio cholerae and escherichia coli was determined by plate counting and direct counting techniques. v. cholerae strains included environmental, clinical, and serotype o1 and non-o1 isolates, whereas e. coli strains included atcc 25922 and a freshly cultured human isolate. recovery of v. cholerae varied significantly with incubation temperature. growth and extended periods of survival occurred in sterile sediments, sterile water ... | 1982 | 7041820 |
| recognition of 2-keto-3-deoxyoctonate in bacterial cells and lipopolysaccharides by the sialic acid binding lectin from the horseshoe crab carcinoscorpius rotunda cauda. | 1982 | 7041896 | |
| [nonselective mechanism of antibody-induced stable antigenic variability in microorganisms. ii. patterns of antigenic variability in cholera vibrios]. | 1982 | 7043962 | |
| molecular cloning of vibrio cholerae enterotoxin genes in escherichia coli k-12. | hybridization probes derived from the a and b subunit genes of the heat-labile enterotoxin (lt) of escherichia coli were used to analyze dna from vibrio cholera strain 569b for cholera toxin gene sequences. southern blot analysis indicated that the cholera toxin a and b subunit genes were each duplicated in the strain. one of the two toxin subunit gene pairs was cloned as a 5.1-kilobase dna insert in plasmid pbr322. e. coli cells carrying the recombinant plasmid pjm17 were shown to produce chole ... | 1982 | 7045877 |
| purification and characterization of the soluble hemagglutinin (cholera lectin)( produced by vibrio cholerae. | the soluble hemagglutinin (ha) (cholera lectin) produced by vibrio cholerae strain ca401 was purified to apparent homogeneity by a sequence of ammonium sulfate fractionation, gel filtration, and preparative isoelectric focusing. soluble ha activity was found to focus at three different pis, 6.3, 5.3, and 4.7. each of the factors migrated as a large-molecular-weight protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under normal conditions, and each, upon heating in sodium dodec ... | 1982 | 7047394 |
| [cholera vibrio culture in a fermenter with automatic control of a number of parameters]. | the cultivation of v. cholerae was carried out in casein broth in a fermenter. 2 kinds of cultivation conditions were compared. the results thus obtained indicate that the cultivation of v. cholerae under the conditions of the controlled level of dissolved oxygen (po2--50%) ensures the optimum conditions for proliferation and the high yield of biomass; it also allows obtaining vaccine whose immunogenic properties are not inferior to those of the who reference vaccine. | 1982 | 7048821 |
| cholera-like illness due to aeromonas sobria. | a thai woman from bangkok was admitted to a hospital in paris for a cholera-like illness. a culture of her "rice-water" diarrhea was negative for vibrio cholerae and enterotoxigenic escherichia coli but was positive for aeromonas sobria. this strain produced enterotoxin, cytolysin, proteolysin, hemolysin, and a cell-rounding factor. acute -and convalescent-phase sera showed an increase in neutralizing antibodies to enterotoxin, cytolysin, and hemolysin. the enterotoxin, which was labile at 100 c ... | 1982 | 7054327 |
| vibrio parahaemolyticus: a major cause of travelers' diarrhea in bangkok. | travelers' diarrhea in bangkok, thailand was investigated in a group of 146 hotel guests who had diarrhea during their stay in the city. the identified pathogenic organisms were vibrio parahaemolyticus in 45 (31%), vibrio cholerae in 1, nonagglutinable vibrio in 1, and entamoeba histolytica in 3. identification of enterotoxigenic escherichia coli was not attempted. the study suggests that vibrio parahaemolyticus is a major cause of travelers' diarrhea in bangkok. | 1982 | 7058975 |
| influenza virus: an nmr study of mechanisms involved in infection. | high resolution 1h-nmr spectroscopy has been used to study the infection of chicken embryo fibroblasts by influenza virus. marked changes in the nmr spectrum occur when infectious influenza virus is introduced into the fibroblasts and these changes appear to depend upon the presence of active neuraminidase (ec 3.2.1.18). a crude preparation of neuraminidase from vibrio cholerae is able to effect similar changes. only minor spectral changes are observed in the absence of culture medium or when th ... | 1982 | 7059616 |
| role of sialic acid in the mobility of membrane proteins containing o-linked oligosaccharides on polyacrylamide gel electrophoresis in sodium dodecyl sulfate. | the major sialoglycoprotein of the human red-cell membrane, glycophorin a, contains 15 o-glycosidically linked oligosaccharides and one n-glycosidic oligosaccharide. the protein shows a decreased mobility on polyacrylamide gel electrophoresis in sodium dodecyl sulfate after neuraminidase treatment of the non-denatured protein. the molecular mechanism behind this phenomenon has been elucidated. neuraminidase treatment of glycophorin a in intact cells or after solubilization in buffers containing ... | 1982 | 7060593 |
| the pathogenicity of nonenterotoxigenic vibrio cholerae serogroup o1 biotype el tor isolated from sewage water in brazil. | nonenterotoxigenic strains no. 1196-78 and no. 1074-78 of vibrio cholerae serogroup o1 (biotype el tor, serotype ogawa) were isolated from sewage water in brazil and fed to 20 volunteers. neither strain caused diarrhea. none of the seven volunteers who ingested ogawa strain no. 1074-78 (10(6) organisms) excreted the organism whereas eight of the 13 volunteers who ingested ogawa strain no. 1196-78 (10(6) or 10(8) organisms) did excrete the organism in their stools. none of 114 stool-culture isola ... | 1982 | 7061878 |
| cholera on the texas gulf coast. | cholera is being increasingly recognized in the gulf coast region. this report describes two cholera cases of classic clinical presentation. both cases were caused by toxigenic vibrio cholerae, one of an 01 serotype and one of a non-01 serotype. vibrio cholerae was also isolated from the home environments of both patients. these findings indicate that cholera continues to be detected on the gulf coast, that non-01 v cholerae infections may be clinically indistinguishable from v cholerae 01 infec ... | 1982 | 7062464 |
| failure of in vivo and in vitro resialosylation of vcn-desialylated erythrocytes in mammalian and non-mammalian species: evidence from agglutination studies with peanut-agglutinin. | 1. in distinction to mammalian erythrocytes, splitting of sialic acids of non-mammalian erythrocytes (chicken and newt) by vibrio cholerae neuraminidase (vcn) does not lead to a dramatic decrease in their viability. 2. one of the possibilities to explain this discrepancy is the ability of chicken and newt desialosylated erythrocytes to repair the enzymatic injury. 3. to test this hypothesis we used the property of peanut agglutinin (pna) to agglutinate desialosylated erythrocytes. 4. desialosyla ... | 1982 | 7067412 |
| kinetics of early cholera infection in the removable intestinal tie-adult rabbit diarrhea model. | the colonization of the small intestine of adult rabbits challenged with 5 x 10(7) cells of vibrio cholerae strain ogawa 395 has been examined in the removable intestinal tie-adult rabbit diarrhea (ritard) model. during the first 6 h of infection, numbers of both free and adherent vibrios increased at a rate representing a generation time of about 71 min. detectable fluid output in response to infection began at about 4 to 5 h postchallenge, and overt diarrhea was observed as early as 11 h. by 8 ... | 1982 | 7068225 |
| the mouse vibriocidal antibody test: a method for monitoring the potency of cholera vaccines. | 1982 | 7068688 | |
| magnitude, kinetics, and duration of vibriocidal antibody responses in north americans after ingestion of vibrio cholerae. | sera from 147 volunteers were examined for vibriocidal antibody by the tube-dilution method before and after they ingested 10(3)-10(6) vibrio cholerae organisms. titers increased significantly after challenge in 97% of 110 persons who excreted v. cholerae. in 12% of the bacteriologically confirmed infections, the titer increased significantly only to the homologous (inaba or ogawa) serotype. levels of vibriocidal antibody decreased substantially between one and six months after challenge, but th ... | 1982 | 7069227 |
| purification and characterization of the mucinase of vibrio cholerae. | mucinase from vibrio cholerae strain ca401 was purified by precipitation with ammonium sulfate and chromatography on a column of biogel p-100 (bio-rad laboratories, richmond, california). ovomucinase, intestinal mucinase, and protease appeared as two peaks of slightly different molecular weights, comigrated in nondenaturing polyacrylamide gel electrophoresis, demonstrated similar patterns of inhibition by heavy metals, and were inhibited by antiserum to purified mucinase. both molecular weight f ... | 1982 | 7069228 |
| is cholera a problem for us travelers? | only ten cases of cholera in us travelers have been reported since the current cholera pandemic began in 1961. no important risk factors were apparent from assessing the age, place of acquisition, duration of mode of travel before infection, or history of cholera vaccination of the travelers who acquired cholera. all of the patients received medical treatment and all recovered; there were no secondary cases. nine of the ten were infected by vibrio cholerae 01 el tor, the strain responsible for t ... | 1982 | 7069880 |
| characterization and distribution of the hemagglutinins produced by vibrio cholerae. | examination of the distribution of cell-associated and soluble hemagglutinins (ha) produced by vibrio cholerae revealed the existence of four different has. a cell-associated mannose-sensitive ha (msha) was produced only by the el tor biotype. this was evident with all el tor strains examined. it appears to be responsible for the ha biotyping differentiation of el tor from classical biotype v. cholerae. the msha had no apparent divalent ion requirement; it was inhibited by d-mannose and d-fructo ... | 1982 | 7076294 |
| protection in rabbits induced by the texas star-sr attenuated a-b+ mutant candidate live oral cholera vaccine. | the avirulent, a-b+, streptomycin-resistant mutant designated texas star-sr, isolated from a virulent, hypertoxinogenic, colonizing strain of vibrio cholerae (ogawa serotype, el tor biotype) and administered intragastrically or intraduodenally in adult rabbits, has been found to induce substantial immunity to subsequent challenge (in ligated intestinal loops) with virulent wild-type cholera vibrios (of both homologous and heterologous biotype and serotype). significant resistance to challenge wi ... | 1982 | 7076295 |
| processing of orally absorbed immunogen. | the absorption of immunogen from the adult mouse intestine was demonstrated. this was shown indirectly by the ability of oral doses of a bacterial antigen to prime an animal for an anamnestic, systemic response, and directly by the ability of absorbed material from the plasma in vivo or everted gut sacs in vitro to prime normal mice when injected intravenously. in all cases, the resultant, systemic response in the recipient animals was qualitatively different (presumably due to iga production) f ... | 1982 | 7076329 |
| repression of the alkaline phosphatase of vibrio cholerae. | the synthesis of alkaline phosphatase by two strains of vibrio cholerae belonging to the inaba and ogawa serotypes has been examined in relation to the phosphate concentration of the culture medium. the synthesis of the enzyme in both strains was repressed in cells grown in the presence of a high concentration of inorganic phosphate. lowering the phosphate content of the growth medium led to a derepression of enzyme activity. the presence of glucose in low phosphate medium stimulated the degree ... | 1982 | 7077294 |
| echinocyte formation induced by potential changes of human red blood cells. | in isotonic 30 mm nacl-saccharose solution, human red blood cells with intact membrane and normal inside ionic content (c-state) indicate a transmembrane potential between +30 mv (at ph 7.4) and +46 mv (at ph 5.1). after treatment with amphotericin b or nystatin as ionophores, a donnan equilibrium (d-state) will be reached with the same potential at ph 5.1 but a sharp drop down to -20 mv will occur at ph 7.4. concerning the erythrocyte shape at these states, a stomatocyte-echinocyte transformati ... | 1982 | 7077650 |
| [characteristics of the cholera vibrios circulating in the 7th pandemic in different territories]. | the study of 491 v. cholerae strains isolated from humans in the endemic foci and the areas of the spread infection during the period of 1969-1979 revealed no essential differences in the properties of the vibrios. the majority of the strains proved to be typical in their main biological properties and belonged to biotype e1 tor, seroval ogawa. among e1 tor strains a comparatively small number of cultures with changed properties were detected. in some states of india classical cholera vibrios an ... | 1982 | 7080752 |
| the uptake of r-type cobalamin-binding protein by isolated rat liver cells. | the uptake of r-type cobalamin-binding protein from human granulocytes and plasma by isolated parenchymal rat liver cells has been studied. when [57co] cyanocobalamin-saturated granulocyte-binding protein or transcobalamin iii was incubated with the liver cells in a concentration of 500 pm, more than 80% of the vitamin was taken up in 1 h. vitamin b-12 bound to plasma transcobalamin i, however, was not taken up unless the protein was desialylated by neuraminidase from vibrio cholerae. the uptake ... | 1982 | 7082685 |
| structural studies of the vibrio cholerae o-antigen. | the dominant part of the o-antigen of vibrio cholerae is a homopolysaccharide composed of (1 leads to 2)-linked 4-amino-4,6-dideoxy-alpha-d-mannopyranosyl (perosaminyl) residues, in amino groups of which are acylated by 3-deoxy-l-glycero-tetronic acid. most of the amino sugar is decomposed during acid hydrolysis. treatment of the polymer with anhydrous hydrogen fluoride, which cleaves the glycosidic linkages but does not cause n-deacylation, followed by acid hydrolysis under mild conditions, pro ... | 1982 | 7083255 |
| effect of certain chemical insecticides on the bacteria associated with the common house-fly musca domestica. | 1982 | 7086231 | |
| [conversion of cholera vibrios to the r form]. | 1982 | 7087808 | |
| [quantitative evaluation of vibrio cholerae colonization and lymphocyte count in small intestine villi of rabbits immunized with vibrio and cholera toxin]. | the influence of parenteral immunization with live virulent vibrios and lipopolysaccharide-containing choleragen toxoid and oral immunization with liver avirulent vibrios on the interaction of v. eltor and the mucous membrane was studied on the ligated loops of the small intestine in 164 rabbits. protection from enterotoxigenicity (the accumulation of fluid) was compared with the suppressive effect on the adhesion and colonization of vibrios. the coefficient of immunization efficacy was determin ... | 1982 | 7090657 |
| [ultrastructure of the small intestine mucosa of gnotobiotic rats with a vibrio cholerae carrier state]. | ultrastructure of the small intestinal mucosa of gnotobiotic rats, carriers of vibrio eltor or v. cholerae was studied. the cytoplasm of enterocytes manifested an increased number of lysosome-like structures and hypertrophy of the golgi complex. unlike v. eltor, v. cholerae induced more intensive extrusion of enterocytes and appreciable infiltration of the epithelial layer with lymphocytes. it is concluded that chronic vibrio-carrier state in gnotobiotic rats is not associated with pathological ... | 1982 | 7093518 |
| protective properties of anticholera antibodies in human colostrum. | a comparative immunological study between two colostrum pools of indian and swedish mothers was carried out to evaluate their protective properties against vibrio cholerae. antibacterial and antitoxin titers were significantly higher in the indian colostrum pool (icp) than in the swedish colostrum pool (scp). antilipopolysaccharide as well as antitoxin antibodies belonged to secretory immunoglobulin a (iga) and igm classes as determined by the enzyme-linked immunosorbent assay. icp could signifi ... | 1982 | 7095856 |
| incidence of vibrio species associated with blue crabs (callinectes sapidus) collected from galveston bay, texas. | bacteria were readily isolated from the hemolymph of a majority (88%) of the blue crabs collected from galveston bay, texas. the hemolymph of most crabs contained moderate (greater than 10(3) bacteria/ml) to heavy (greater than (10(5) bacteria/ml) infections. large variances were observed in the bacterial number associated with individual crabs, but no significant difference was observed between the mean bacterial levels in the hemolymph of crabs collected during different seasons of the samplin ... | 1982 | 7103475 |
| antibodies to vibrio cholerae somatic and enterotoxin antigens in human colostrum. | 1982 | 7106887 | |
| the incidence of vibrio cholerae in water, animals and birds in kent, england. | 1982 | 7107541 | |
| adsorption kinetics of laterally and polarly flagellated vibrio. | the adsorption of laterally and polarly flagellated bacteria to chitin was measured, and from the data obtained, a modified langmuir adsorption isotherm was derived. results indicated that the adsorption of laterally flagellated vibrio parahaemolyticus follows the langmuir adsorption isotherm, a type of adsorption referred to as surface saturation kinetics, when conditions are favorable for the production of lateral flagella. when conditions were not favorable for the production of lateral flage ... | 1982 | 7107559 |
| molecular epidemiology of vibrio cholerae in the u.s. gulf coast. | enterotoxigenic strains of vibrio cholerae o-1, biotype el tor, isolated from a case of cholera in texas in 1973, an outbreak of cholera in louisiana in 1978, and louisiana sewage samples in 1980 and 1981 were analyzed for their genetic similarities. chromosomal dna was isolated from each strain, digested with restriction endonuclease, and analyzed by the southern blot technique. a radioactive probe consisting of escherichia coli heat-labile enterotoxin dna detected cholera toxin gene sequences ... | 1982 | 7107852 |
| development of an enzyme-linked immunosorbent assay for studying vibrio cholerae cell surface antigens. | an enzyme-linked immunosorbent assay for the measurement of antibodies directed against cell surface antigens of vibrio cholerae (csa elisa) was developed. nan3-killed whole cells of v. cholerae, adsorbed to polystyrene tubes, were used as immobilized antigens. the assay was capable of detecting antibodies directed against lipopolysaccharide and non-lipopolysaccharide surface antigens. in addition, the csa elisa was capable of detecting non-vibriocidal antibody. an antiserum raised in rabbits by ... | 1982 | 7107860 |
| salmonellosis at rural and urban clinics in bangladesh: epidemiologic and clinical characteristics. | the authors studied the frequency of diarrheal illness associated with non-typhi salmonella at two clinics in bangladesh for the years 1977-1979. non-typhi salmonellae were isolated from 0.29% of fecal specimens or rectal swabs in an urban area and 0.26% of similar specimens in a rural area; the frequency of isolations peaked in the summer months. isolations of shigella and vibrio cholerae were much more common than salmonella. only two of 50 salmonella isolates were resistant to more than one a ... | 1982 | 7114037 |
| kinetics of vibrio cholerae sialidase action on gangliosidic substrates at different supramolecular-organizational levels. | g(d1a), g(d1b) and g(t1b) gangliosides were dispersed in the following membrane-mimicking systems: (a) homogeneous micelles; (b) mixed micelles with g(m1) ganglioside (which is resistant to the enzyme action), triton x-100 or bovine serum albumin; (c) small unilamellar vesicles of egg phosphatidylcholine. the effect of dispersion on sialic acid release by vibrio cholerae sialidase was studied. as reference substrates freely interacting with the enzyme the lipid-free carbohydrates of g(d1a) and 3 ... | 1982 | 7115311 |
| biosynthesis and localization of gangliosides in cultured cells. | mouse neuroblastoma n18 cells contain a homologous series of gangliosides (gm3, gm2, gm1, and gd1a) which constitute a biosynthetic pathway. when added to the culture medium, tritium-labeled palmitate, galactose, and n-acetylmannosamine were incorporated into these gangliosides. incorporation of [3h]galactose into all four gangliosides was detected by 5 min and continued at essentially linear rates for several hours. when the cells were treated with vibrio cholerae neuraminidase, the amounts of ... | 1982 | 7115666 |
| fatal non-01 vibrio cholerae septicemia in chronic lymphocytic leukemia. | 1982 | 7117796 | |
| ecology of vibrio species, including vibrio cholerae, in natural waters in kent, england. | 1982 | 7118752 | |
| density-dependent changes in gangliosides and sialidase activity of murine neuroblastoma cells. | density-dependent changes in ganglioside composition, vibrio cholerae neuraminidase (vcn)-susceptible sialyl residues, and membrane-associated sialidase activity were determined for the cholinergic murine neuroblastoma cell line s20y. a decrease in total ganglioside sialic acid and vcn-releasable sialic acid was observed with increasing cell density. gm3 was the major ganglioside component of preconfluent s20y cells, whereas gd1a was predominant in postconfluent cells. sialidase activity increas ... | 1982 | 7119793 |
| not one but four possible oral vaccines for cholera under study. | 1982 | 7120612 | |
| cholera in sardinia: imported infection or endemic focus? | 1982 | 7121299 | |
| role of temperate phage in determining lytic phage sensitivity and serotype of vibrio cholerae. | the effect of lysogenization with five temperate phages from various sources on serotype and lytic phage sensitivity was investigated in six cultures of vibrio cholerae of both classical and el tor biotypes. no changes in serotype or in classical phage sensitivity in the classical biotype were observed. four of the temperate phages were homoimmune and induced resistance to one of the el tor typing phages, e3, thereby causing a type change in el tor strains. the sensitivity to the other phages wa ... | 1982 | 7129638 |
| biotype-specific restriction and modification of dna in vibrio cholerae. | by using vibrio cholerae typing phages it was possible to demonstrate that within v. cholerae of the o-1 serotype there are at least two biotype-specific dna restriction and modification systems. | 1982 | 7130366 |
| studies on the growth of vibrio cholerae biotype eltor and biotype classical in foods. | the growth of vibrio cholerae biotype eltor and biotype classical was studied in a range of cooked foods, shellfish and raw vegetables, incubated at 22 degrees, 30 degrees and 37 degrees c. both biotypes grew in all cooked foods but growth was not demonstrated on raw shellfish. the organism multiplied on some vegetables to levels of the order of 10(6)/g. the classical biotypes of v. cholerae showed a longer lag period than the eltor biotype in some foods particularly when incubated at 22 degrees ... | 1982 | 7130700 |
| [polylysogeny in vibrio eltor]. | 1982 | 7135928 | |
| structural investigations on the lipopolysaccharide isolated from vibrio cholera ogawa g-2102. | 1982 | 7139661 | |
| effect of chemical and heat inactivation on the antigenicity and immunogenicity of vibrio cholerae. | the effects of heat and chemical inactivation on the antigenicity and immunogenicity of vibrio cholerae 1418 in rabbits were studied. v. cholerae 1418 was inactivated with heat and chemical inactivants (phenol or formalin) alone or in combination. enzyme-linked immunoassay systems employing whole cells of v. cholerae 1418, lipopolysaccharide, or flagella as immobilized antigens were used to measure the antibody response (immunoglobulins g and m) after parenteral immunization of rabbits with vari ... | 1982 | 7141690 |
| ecology and importance of bacterial' species of the family vibrionaceae. | the bacterial species of the family vibrionaceae, particularly the species of the genus aeromonas (a. hydrophila and a. punctata and their subspecies were found to be relatively common in the running surface water in czechoslovakia (the occurrence of a. salmonicida species was not studied). the strains of the so-called nag-vibrios (vibrio cholerae unrelated to the cholera vibrio 0 group 1) were also relatively frequent, but the positive isolates of plesiomonas shigelloides were sporadic. the abo ... | 1982 | 7142689 |
| role of bacterial adhesion in the pathogenesis of cholera. | in studies with the adult-rabbit ileal-loop model, antibodies to the lipopolysaccharide somatic-antigen component of vibrio cholerae gave passive protection against challenge with live v. cholerae. the antisomatic antibodies had no effect on bacterial proliferation and toxin production either in vivo or in vitro; after challenge, antibody-protected and non-protected rabbit ileal loops developed almost identical amounts of cholera toxin and numbers of v. cholerae. the protection could be correlat ... | 1982 | 7143425 |
| role of somatic antigen of vibrio cholerae in adhesion to intestinal mucosa. | the in-vitro adhesion of vibrio cholerae to intestinal mucous membrane was studied in isolated adult-rabbit ileal loops. antisomatic antiserum against v. cholerae inaba could inhibit adhesion of three different strains of v. cholerae inaba but had no effect on the adhesion of two different strains of enterotoxigenic nag vibrios. the antiserum's bacterial agglutinin titre was 320, its anti-inaba lipopolysaccharide (lps) titre was 16 000 and its anti-flagellar antibody titre was 3200. conversely, ... | 1982 | 7143426 |
| vibrio cholerae infections. | 1982 | 7143667 | |
| fecal steroids in diarrhea: iv. cholera. | fecal bile acid and neutral sterol patterns were studied in eight healthy adult volunteers who were challenged with vibrio cholerae classical ogawa 395 strain in the course of vaccine development studies. bacterial 7 alpha-dehydroxylation of cholic and chenodeoxycholic acids was not altered during experimentally induced cholera diarrhea, despite the fact that fecal weight in g/day (wet wt) was increased greatly during diarrhea (1913 +/- 390 vs 161 +/- 11 in controls, p less than 0.005). consiste ... | 1982 | 7144450 |
| [effect of certain factors on vibrio cholerae multiplication in fermenter]. | the influence of the level of dissolved oxygen, as well as the phase of growth and the amount of inoculum introduced, on the growth of bacterial population in the process of the cultivation of cholera vibrios in the fermenter "novopalias" has been studied. the results of this study indicate that the cultivation of cholera vibrios with the level of dissolved oxygen constituting 50% of the maximum air saturation of the broth ensures a better yield of the biomass than cultivation with the level of ... | 1982 | 7148231 |
| endemic cholera in rural bangladesh, 1966-1980. | since 1963, the international centre for diarrhoeal disease research, bangladesh (icddr,b), formerly the cholera research laboratory, has maintained a field station in matlab to treat patients from a surveillance population of 240,000 who have cholera and other diarrheal diseases. since 1966, the authors have analyzed hospital records of 7141 surveillance-area patients culture-positive for v. cholerae 01 to relate the seasonality, age and sex distribution, and geographic trends with hypotheses c ... | 1982 | 7148820 |
| role of gangliosides in reception of influenza virus. | the ganglioside composition of ehrlich ascites carcinoma (eac) cells and the role of the individual gangliosides in binding and penetration into the cell of influenza virus were determined. eac gangliosides identical with or close to gm3, gm2, gm1, gt1a and gt1b were characterized by thin-layer chromarography, compositional analyses, methylation analysis and mass-spectrometry. the ganglioside uptake capacity of native and neuraminidase-treated eac cells was studied with tritium-labeled gangliosi ... | 1982 | 7151789 |
| characterization of the lipopolysaccharide from vibrio cholerae 395 (ogawa). | the chemical structure and biological properties of the lipopolysaccharide (lps) from vibrio cholerae 395 (ogawa), isolated by the phenol-water procedure, were studied. upon acid hydrolysis, the lps was split into its polysaccharide and lipid a moieties. the polysaccharide contained both neutral (glucose, heptose, fructose) and amino (glucosamine, quinovosamine) sugars. the lps contained the acid-labile amino sugar, 4-amino-arabinose, which was absent in the inaba serotype of v. cholerae. the lp ... | 1982 | 7152669 |
| vibrio cholerae o1 isolated in kenya. | biological and serological analyses of 272 isolates of vibrio cholerae o1 from six epidemics and from a few sporadic cases in kenya were carried out. all of the isolates were identified as v. cholerae biotype e1 tor, and 210 out of 272 isolates were hemolytic as examined by feeley's method. | 1982 | 7153323 |
| [characteristics of the formation, persistence and reversion of cholera vibrio l forms in the body of experimental animals]. | 1982 | 7154997 | |
| muscle glycolipids in inherited muscular dystrophy of chickens. | gangliosides and neutral glycolipids of muscles from normal and dystrophic chickens were studied. total glycolipid content of the degenerating muscles was higher than the normal muscles. in addition, the myopathic muscles contained a ganglioside which was absent in the unaffected muscles from normal and dystrophic chickens. based on the thin-layer chromatographic mobility, treatment with neuraminidases from vibrio cholerae and arthrobacter ureafaciens, and reactivity of the asialo-derivative tow ... | 1982 | 7160480 |
| a coagglutination test to detect vibrio cholerae in feces alkaline peptone water cultures. | 1982 | 7163843 | |
| [determination of the sensitivity of nag-vibrio to antibiotics by the methods of serial dilution in agar and agar diffusion]. | the methods of serial dilutions and agar diffusion in medium agv were used in the study on sensitivity of 200 nag-vibrio strains to 10 antibiotics. the regression equations showing the relation between the mic log2 and the diameter of the zones of the vibrio growth inhibition around the standard discs with the antibiotics were estimated. an evaluation table was developed for semiquantitative interpretation of the diameters of the inhibition zones of the vibrio growth when the agar diffusion meth ... | 1982 | 7165291 |
| [behavior of el tor cholera vibrio in various environments, especially in urban water system and coastal sea water]. | 1982 | 7166587 | |
| intra-familial transmission of vibrio cholerae biotype e1 tor in calcutta slums. | 1982 | 7169236 | |
| soluble haemagglutinin of classical vibrios: isolation and protection against cholera by its antibodies. | soluble haemagglutinin was isolated from a late culture supernatant of classical vibrio cholerae grown in casein-peptone broth with aeration by saturated ammonium sulphate precipitation in the cold, followed by gel filtration, electrophoresis, ether extraction and electrophoresis respectively. the antigen was found to consist of three protein fractions with different electrophoretic mobilities. antibodies to these fractions afforded low but significant protection against oral challenges with hom ... | 1982 | 7170647 |
| neuraminidase- and benzalkonium chloride-dependent inhibition of basic peptide-induced rat mast cell secretion. | two types of inhibition of basic peptide-induced rat mast cell secretion are reported. pretreatment of rat peritoneal mast cells with vibrio comma neuraminidase, an enzyme which cleaves sialic acid from oligosaccharides, led to inhibition of 5-hydroxytryptamine release induced by the basic peptides polylysine, corticotropin 1-24 and a decapeptide sequence of human ige. inhibition was similarly observed when mast cells were challenged in the presence of the cationic cell membrane-active substance ... | 1982 | 7173980 |
| the effects of nutrient limitation and growth rate in the chemostat on the immunogenicity of vibrio cholerae. | 1982 | 7174561 | |
| enterotoxin production, dna repair and alkaline phosphatase of vibrio cholerae before and after animal passage. | 1982 | 7175493 | |
| endotoxaemia in vibrio el tor cholera. | endotoxaemia and endotoxin-induced changes were sought in nigerian patients presenting with cholera/diarrhoea. the organism was vibrio cholerae, bio-type el tor, serotype hikojima. the limulus amoebocyte lysate gelation test was used qualitatively by the clot method, whilst a spectrophotometric method was used quantitatively to measure endotoxin levels. 25 acutely ill patients tested had detectable endotoxaemia by the escherichia coli endotoxin standard. the highest endotoxin level was found in ... | 1982 | 7179411 |
| characteristics of the indigenous vibrio cholerae: classical versus eltor biotypes. | 1982 | 7184950 | |
| [isolation and significance of vibrio cholera nag]. | after the isolation of two vibrio cholerae nag from the stools of two tourists, the authors researched vibrio in people coming home from particular countries and in resident people. the research was extended to the water of varese lake after another isolation from a fisher who had fished, cooked and eaten the lake fish. problems concerning the classification of vibrio and their presence in the environment are examined. | 1982 | 7187344 |
| [microbiological and chemical studies of the water from lake varese: isolation of vibrio cholerae non 0-1]. | the authors examined varese lake water for the research of vibrios. the research was extended to the determination of fecal coliforms, salmonellae and chemical parameters. three strains of vibrio cholerae non 0-1 were isolated in three moore tampons among 18 tampons examined . any association between chemical parameters, determination of fecal coliforms and the presence of salmonellae and vibrio cholerae non 0-1 was impossible. | 1982 | 7188267 |